CN214032446U - Cell filtering device - Google Patents

Cell filtering device Download PDF

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Publication number
CN214032446U
CN214032446U CN202022562724.9U CN202022562724U CN214032446U CN 214032446 U CN214032446 U CN 214032446U CN 202022562724 U CN202022562724 U CN 202022562724U CN 214032446 U CN214032446 U CN 214032446U
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China
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liquid storage
storage cylinder
connecting pipe
filter
drainage tube
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CN202022562724.9U
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Chinese (zh)
Inventor
林俊堂
付康
王现伟
任新华
杨远方
亢星亮
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Henan Intercell Biology Technology Co ltd
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Henan Intercell Biology Technology Co ltd
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Abstract

The utility model relates to a cell filtering device, including liquid storage cylinder and filter flask, sliding connection has the piston in the liquid storage cylinder, the one end of piston is fixed with the installation pole, the installation pole extends to outside the liquid storage cylinder, the upper portion intercommunication of liquid storage cylinder one side has the drainage tube, the drainage tube sets up in the liquid storage cylinder both sides with the installation pole relatively, the intercommunication has the connecting pipe between the lower part of liquid storage cylinder one side and the filter flask top, connecting pipe and drainage tube are located the homonymy of liquid storage cylinder, the connecting pipe, all be equipped with the flow stopping clamp on the drainage tube, be equipped with first check valve on the drainage tube, be equipped with the second check valve on the connecting pipe, fixedly connected with filter screen can be dismantled to the top of filter flask inside, the bottom intercommunication of filter flask has the fluid-discharge tube, be equipped with the flow stopping valve on the fluid-discharge tube, the fluid-discharge tube bottom is equipped with the rubber cap. Simple structure, convenient operation, the filter effect is good, and whole filtering operation is gone on under sealed condition, has reduced the contaminated probability of cell.

Description

Cell filtering device
Technical Field
The utility model belongs to the technical field of it is biological, especially relate to a cell filter equipment.
Background
With the continuous development of scientific technology, research on stem cells and regenerative medicine has become the most attractive field in natural science. The perfect improvement of stem cell theory and the rapid development of technology also provide a new research direction for the fields of disease treatment, biomedicine and the like. Currently, stem cells widely used in clinical applications include: cord blood stem cells, mesenchymal stem cells, neural stem cells, and the like. Stem cell transplantation has been gradually applied to the treatment of malignant solid tumors, tissue and organ repair, nervous system injury and other diseases. In the clinical application process of the cord blood stem cells, collected whole cord blood needs to be centrifuged, plasma at the upper layer and red blood cells at the lower layer are respectively removed after centrifugation, and stem cells at the middle layer are purified and collected. Before carrying out centrifugal separation, in order to guarantee centrifugal effect, the layering of being convenient for improves the purity of stem cell, often needs to carry out prefilter to stem cell, gets rid of the cell group that contains in the blood, reduces the viscosity of blood, guarantees going on smoothly of follow-up centrifugal process.
However, cell filter equipment among the prior art often installs the cell filter sieve in the inside top of cell sap collection pipe, then carry blood to the cell sap collection pipe through the opening part at cell sap collection pipe top in, after the filtration, need take out the cell filter sieve from cell sap collection pipe inside, then empty out the cell sap, this kind of filtration mode, not only the filter effect is poor, and the cell sap contacts with external environment, whole process all needs to go on in sterile environment, extremely strict to the operating environment requirement, the operation is inconvenient, the contaminated problem of cell appears very easily.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a cell filtering device for solve the cell filtering device among the prior art, the filter effect is poor, inconvenient operation and the easy contaminated technical problem of cell.
In order to solve the technical problem, the utility model discloses the technical scheme who adopts is: a cell filtering device is provided, which comprises a liquid storage cylinder and a filter bottle which are arranged at intervals, a piston is connected inside the liquid storage cylinder in a sliding manner, one end of the piston is fixedly connected with a horizontally arranged installation rod, one end of the installation rod, which is far away from the piston, penetrates through the liquid storage cylinder and extends to the outside of the liquid storage cylinder, the upper part of one side of the liquid storage cylinder is communicated with a drainage tube, the drainage tube and the installation rod are arranged at two sides of the liquid storage cylinder oppositely, a connecting pipe is communicated between the lower part of one side of the liquid storage cylinder and the top of the filter bottle, the connecting pipe and the drainage tube are positioned at the same side of the liquid storage cylinder, a flow stopping clamp is respectively arranged on the connecting pipe and the drainage tube, a first one-way valve for preventing liquid from flowing back from the liquid storage cylinder to the drainage tube is fixed on the connecting pipe, a second one-way valve for preventing liquid from flowing back from the connecting pipe to the liquid storage cylinder is fixed on the connecting pipe, a filter screen is detachably and fixedly connected above the inside of the filter bottle, a liquid discharge pipe which extends downwards is communicated with the bottom of the filter bottle, the liquid discharge pipe is provided with a stop valve, and the bottom of the liquid discharge pipe is detachably and fixedly connected with a rubber cap.
The bottom intercommunication of connecting pipe has the branch pipe that two symmetries set up, and the branch pipe constitutes the type of falling Y with the connecting pipe, and the bottom of two branch pipes all runs through the filter flask and extends to the inside top of filter flask, and the liquid outlet has been seted up to the bottom of branch pipe.
The cross section of the filter screen is M-shaped, the filter screen is positioned below the branch pipe, and the two peaks of the filter screen correspond to the two liquid outlets one by one.
The periphery of filter screen bottom is fixed with downwardly extending's fixture block, is fixed with the draw-in groove with fixture block matched with on the inner wall of filter flask, and the filter screen passes through the fixture block and clamps inside the filter flask.
An elastic ball is fixed on the connecting pipe, the elastic ball is of an internal hollow structure, the interior of the elastic ball is communicated with the connecting pipe, and the inner diameter of the elastic ball is larger than that of the connecting pipe.
The fixed cover in periphery of piston is equipped with rubber seal, rubber seal and the inner wall sliding connection of liquid storage cylinder.
One end of the mounting rod, which is far away from the piston, is fixedly connected with a vertically arranged handle.
The bottom of the filter flask is fixed with a V-shaped groove, and the liquid discharge pipe is fixedly connected to the bottom of the V-shaped groove.
The filter flask is made of transparent material.
The utility model has the advantages that: (1) the structure is simple, the liquid storage cylinder, the piston and the filter bottle are arranged, when the device is used, the piston is pulled outwards, the whole umbilical cord blood in the blood bag is extracted into the liquid storage cylinder through the drainage tube, then the piston is pushed, the whole umbilical cord blood in the liquid storage cylinder is conveyed into the filter bottle through the connecting tube for filtering operation, the device is convenient to use, the whole filtering process is carried out under a sealed condition, cells are prevented from contacting with the external environment, and the probability of cell pollution is reduced; (2) the cross section of the filter screen is M-shaped, two peaks of the filter screen correspond to the two liquid outlets one by one, the filtering area and the filtering speed are increased, the filter screen is prevented from being blocked, and the filtering efficiency is improved; (3) set up the elastic ball, when the connecting pipe blockked up, can dredge the connecting pipe through kneading the elastic ball, avoid influencing the normal clear of filtering operation because of the connecting pipe blocks up.
Drawings
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is an enlarged view of a point a in fig. 1.
Reference numerals: the device comprises a liquid storage cylinder 1, a filter bottle 2, a piston 3, a rubber sealing ring 4, a mounting rod 5, a handle 6, a drainage tube 7, a connecting tube 8, a flow stopping clamp 9, a first one-way valve 10, a second one-way valve 11, an elastic ball 12, a filter screen 13, a liquid discharge tube 14, a flow stopping valve 15, a rubber cap 16, a V-shaped groove 17, a branch tube 18, a liquid outlet 19, a clamping block 20 and a clamping groove 21.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention clearer, embodiments of the present invention are described in further detail below with reference to the accompanying drawings.
As shown in fig. 1, the utility model discloses a liquid storage cylinder 1 and filter flask 2 that the interval set up, the internally mounted of liquid storage cylinder 1 has piston 3, and the fixed cover in periphery of piston 3 is equipped with rubber seal 4, rubber seal 4 and liquid storage cylinder 1's inner wall sliding connection. Set up rubber seal 4, make between piston 3 and the 1 inner wall of liquid storage cylinder laminating inseparabler, increase the leakproofness between piston 3 and the liquid storage cylinder 1. The one end fixed connection of piston 3 has the installation pole 5 that the level set up, and installation pole 5 sliding connection is inside 1 liquid storage cylinder, and the one end that installation pole 5 deviates from piston 3 extends to 1 outside of liquid storage cylinder and the handle 6 of the vertical setting of fixedly connected with, through outside pulling or inside promotion handle 6, realizes piston 3 at the inside reciprocating motion of liquid storage cylinder 1. The upper portion intercommunication of liquid storage barrel 1 one side has drainage tube 7, the blood bag (not shown in the figure) is connected to the free end of drainage tube 7, drainage tube 7 sets up in liquid storage barrel 1 both sides with installation pole 5 relatively, the intercommunication has connecting pipe 8 between the lower part of liquid storage barrel 1 one side and the top of filter flask 2, connecting pipe 8 and drainage tube 7 are located the homonymy of liquid storage barrel 1, connecting pipe 8, all be equipped with on the drainage tube 7 and end the flow clamp 9, be fixed with on the drainage tube 7 and be used for preventing liquid from the first check valve 10 of liquid storage barrel 1 to drainage tube 7 refluence, be fixed with on the connecting pipe 8 and be used for preventing liquid from the second check valve 11 of connecting pipe 8 to liquid storage barrel 1 refluence. An elastic ball 12 is fixed on the connecting pipe 8, the elastic ball 12 is of an internal hollow structure, the interior of the elastic ball 12 is communicated with the connecting pipe 8, and the inner diameter of the elastic ball 12 is larger than that of the connecting pipe 8. Set up elastic ball 12, when connecting pipe 8 is inside to be blockked up, can dredge connecting pipe 8 through kneading elastic ball 12, avoid connecting pipe 8 to block up. The filter bottle 2 is made of transparent materials, and transparent glass or transparent hard plastics can be selected, so that the condition inside the filter bottle 2 can be observed conveniently. The top of the filter bottle 2 is detachably and fixedly connected with a sealing cover, the upper part inside the filter bottle 2 is detachably and fixedly connected with a filter screen 13, the bottom of the filter bottle 2 is communicated with a liquid discharge pipe 14 extending downwards, the liquid discharge pipe 14 is provided with a check valve 15, and the bottom of the liquid discharge pipe 14 is in threaded connection with a rubber cap 16. Rubber cap 16 prevents contamination of discharge pipe 14. The bottom of the filter flask 2 is fixed with a V-shaped groove 17 for facilitating liquid drainage and avoiding waste of cells due to incomplete liquid drainage, and a liquid drainage pipe 14 is fixedly connected to the bottom of the V-shaped groove 17.
The bottom intercommunication of connecting pipe 8 has the branch pipe 18 that two symmetries set up, and branch pipe 18 downwardly extending, branch pipe 18 and connecting pipe 8 constitute the type of falling Y, and the bottom of two branch pipes 18 all runs through filter flask 2 and extends to the inside top of filter flask 2, and liquid outlet 19 has been seted up to the bottom of branch pipe 18. The cross section of the filter screen 13 is M-shaped, the filter screen 13 is located below the branch pipe 18, and two peaks of the filter screen 13 correspond to the two liquid outlets 19 one by one. The filtering area and the filtering speed are increased, the filter screen 13 is prevented from being blocked, and the filtering efficiency is improved.
As shown in fig. 2, a fixture block 20 extending downward is fixed on the periphery of the bottom of the filter screen 13, a clamping groove 21 matched with the fixture block 20 is fixed on the inner wall of the filter bottle 2, the fixture block 20 and the clamping groove 21 are both in an annular structure, and the filter screen 13 is clamped inside the filter bottle 2 through the fixture block 20. The filter screen 13 is convenient to install and replace, and the operation is convenient.
The utility model discloses a theory of operation: the free end of the drainage tube 7 is communicated with a blood bag to be filtered, the piston 3 is pulled outwards to generate negative pressure inside the liquid storage cylinder 1, blood in the blood bag enters the liquid storage cylinder 1 through the drainage tube 7 under the action of pressure difference, then the piston 3 is pushed, the blood in the liquid storage cylinder 1 is conveyed into the filter bottle 2 through the connecting tube 8, and the blood flows into the bottom of the filter bottle 2 after being filtered by the filter screen 13 and is discharged outwards through the liquid discharge tube 14. When the connection tube 8 is clogged, the connection tube 8 can be unclogged by kneading the elastic ball 12. After the filtration is finished, when the filter screen 13 needs to be cleaned, the sealing cover at the top of the filter bottle 2 can be opened, and the fixture block 20 can be taken out from the clamping groove 21. Convenient operation, the filter effect is good, and whole filtering process does not contact with external environment, has reduced the contaminated probability of cell.
The above embodiments are for the description of the present invention, not for the limitation of the present invention, and any scheme after simply changing the present invention all belongs to the protection scope of the present invention.

Claims (9)

1. A cell filtration device, characterized by: the liquid storage device comprises a liquid storage cylinder and a filter bottle which are arranged at intervals, a piston is connected inside the liquid storage cylinder in a sliding manner, one end of the piston is fixedly connected with a horizontally arranged installation rod, one end of the installation rod, which is far away from the piston, penetrates through the liquid storage cylinder and extends to the outside of the liquid storage cylinder, the upper part of one side of the liquid storage cylinder is communicated with a drainage tube, the drainage tube and the installation rod are arranged at two sides of the liquid storage cylinder oppositely, a connecting pipe is communicated between the lower part of one side of the liquid storage cylinder and the top of the filter bottle, the connecting pipe and the drainage tube are positioned at the same side of the liquid storage cylinder, flow stopping clamps are respectively arranged on the connecting pipe and the drainage tube, a first one-way valve for preventing liquid from flowing back from the liquid storage cylinder to the drainage tube is fixed on the drainage tube, a second one-way valve for preventing liquid from flowing back from the connecting pipe to the liquid storage cylinder is fixed on the connecting pipe, a filter screen is detachably and fixedly connected above the inside of the filter bottle, and a liquid discharge tube extending downwards is communicated with the bottom of the filter bottle, the liquid discharge pipe is provided with a stop valve, and the bottom of the liquid discharge pipe is detachably and fixedly connected with a rubber cap.
2. The cell filtration device of claim 1, wherein: the bottom intercommunication of connecting pipe has the branch pipe that two symmetries set up, and the branch pipe constitutes the type of falling Y with the connecting pipe, and the bottom of two branch pipes all runs through the filter flask and extends to the inside top of filter flask, and the liquid outlet has been seted up to the bottom of branch pipe.
3. A cell filtration device according to claim 2, wherein: the cross section of the filter screen is M-shaped, the filter screen is positioned below the branch pipe, and the two peaks of the filter screen correspond to the two liquid outlets one by one.
4. A cell filtration device according to claim 3, wherein: the periphery of filter screen bottom is fixed with downwardly extending's fixture block, is fixed with the draw-in groove with fixture block matched with on the inner wall of filter flask, and the filter screen passes through the fixture block and clamps inside the filter flask.
5. A cell filtration device according to claim 3, wherein: an elastic ball is fixed on the connecting pipe, the elastic ball is of an internal hollow structure, the interior of the elastic ball is communicated with the connecting pipe, and the inner diameter of the elastic ball is larger than that of the connecting pipe.
6. A cell filtration device according to claim 3, wherein: the fixed cover in periphery of piston is equipped with rubber seal, rubber seal and the inner wall sliding connection of liquid storage cylinder.
7. A cell filtration device according to claim 3, wherein: one end of the mounting rod, which is far away from the piston, is fixedly connected with a vertically arranged handle.
8. A cell filtration device according to any one of claims 1 to 7, wherein: the bottom of the filter flask is fixed with a V-shaped groove, and the liquid discharge pipe is fixedly connected to the bottom of the V-shaped groove.
9. A cell filtration device according to any one of claims 1 to 7, wherein: the filter flask is made of transparent material.
CN202022562724.9U 2020-11-09 2020-11-09 Cell filtering device Active CN214032446U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022562724.9U CN214032446U (en) 2020-11-09 2020-11-09 Cell filtering device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022562724.9U CN214032446U (en) 2020-11-09 2020-11-09 Cell filtering device

Publications (1)

Publication Number Publication Date
CN214032446U true CN214032446U (en) 2021-08-24

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Application Number Title Priority Date Filing Date
CN202022562724.9U Active CN214032446U (en) 2020-11-09 2020-11-09 Cell filtering device

Country Status (1)

Country Link
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117305078A (en) * 2023-09-25 2023-12-29 成都赛恩吉诺生物科技有限公司 Tissue dissociation method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN117305078A (en) * 2023-09-25 2023-12-29 成都赛恩吉诺生物科技有限公司 Tissue dissociation method

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