CN216864159U - Cord blood stem cell separator - Google Patents

Abstract

The utility model provides a cord blood stem cell separation device, which comprises a separation cavity and an upper cover for covering the separation cavity, wherein the upper cover is provided with a first sample adding hole and a second sample adding hole, the first sample adding hole is communicated with a filter cavity positioned at the upper part of the separation cavity, the side surface of the separation cavity is fixed to a support frame, the lower part of the separation cavity is provided with a piston, the side surface of the bottom of the separation cavity is provided with an outlet pipe, and the bottom of the piston is provided with a bottom support rod; the bottom surface of the supporting frame is a cylindrical bottom supporting plate, the side surface of the supporting frame is an annular side supporting plate, and the bottom of the side supporting plate is fixed to the edge of the bottom supporting plate. The umbilical cord blood stem cell separation device is provided with the piston to effectively separate umbilical cord blood stem cells, a complex three-way pipe and a storage bag connected with a plurality of plastic pipes are abandoned, and the device is simple in structure and convenient to operate; the whole structure is a pipe column type structure, the separation effect is good, and the applicability is strong; and the reagent can be stored in the reagent tube according to the preset specification in advance, so that the reagent measuring process is omitted, the operation time is saved, and the separation efficiency is improved.

Description

Cord blood stem cell separator

Technical Field

The utility model relates to the technical field of umbilical cord blood stem cell experimental equipment, in particular to an umbilical cord blood stem cell separation device.

Background

Cord blood contains a large number of stem cells, including hematopoietic stem cells and various other stem cells, collectively called cord blood stem cells, and currently, there are a plurality of clinical studies on cord blood in regenerative medicine, aiming at promoting the repair of damages to tissues and organs of a body by using the cord blood stem cells.

Since cord blood contains many components, it is necessary to separate and purify cord blood stem cells. At present, most of separation bags for separating cord blood stem cells are simple in structure, are multi-connected bags connected by a plurality of plastic bags, a plurality of three-way pipes and a plurality of plastic pipes, are complex to operate, are easy to cause misoperation, and store separation liquid in wrong bags, and are not ideal in separation effect and high in price. Therefore, there is an urgent need for a cord blood stem cell separation device having a simple structure, convenient operation and good separation effect.

SUMMERY OF THE UTILITY MODEL

The utility model aims to provide an umbilical cord blood stem cell separation device, which adopts a single separation cavity structure, transfers separation liquid for storage by operating a piston after separation is finished, and has the advantages of simple structure and convenience in operation.

In order to achieve the purpose, the utility model adopts the following technical scheme:

the utility model provides a cord blood stem cell separation device, which comprises a separation cavity and an upper cover used for covering the separation cavity, wherein the upper cover is provided with a first sample adding hole and a second sample adding hole, the first sample adding hole is communicated with a filter cavity positioned at the upper part of the separation cavity, the side surface of the separation cavity is fixed to a support frame, the lower part of the separation cavity is provided with a piston, the side surface of the bottom of the separation cavity is provided with an outlet pipe, and the bottom of the piston is provided with a bottom support rod; the bottom surface of the support frame is a cylindrical bottom support plate, the side surface of the support frame is an annular side support plate, and the bottom of the side support plate is fixed to the edge of the bottom support plate.

In order to further optimize the technical scheme, the technical measures adopted by the utility model also comprise:

furthermore, the upper cover can be clamped to the top of the separation cavity, and the first sample adding hole and the second sample adding hole are internally provided with corresponding sealing plugs; the first sample adding hole is used for adding collected umbilical cord blood, and the second sample adding hole is used for adding hydroxyethyl starch. Preferably, the upper cover is provided with a handle, and the sealing plug is provided with a pull ring.

Further, the filter chamber is provided with a conventional filter element comprising: a filtering membrane, an isolation net, a guide plate, a guide opening, a human-shaped membrane and the like.

Furthermore, a first groove is formed in the middle of the bottom of the piston, a second groove is formed in the middle of the top of the bottom support plate, and the upper end and the lower end of the bottom support rod are correspondingly clamped into the first groove and the second groove respectively; preferably, the support bar has a certain elasticity.

Furthermore, the separation chamber is of a transparent funnel type structure and comprises an upper cylinder, a lower conical body and a bottom funnel neck, the bottom of the upper cylinder is fixed with the top of the side supporting plate, and a piston is arranged in the bottom funnel neck. Preferably, a sealing ring is arranged between the piston and the bottom funnel neck.

Further, the elevation of the piston top seal is greater than the elevation of the outlet tube with the bottom support bar supported or removed.

Furthermore, a waste liquid port is formed in the bottom of the bottom funnel neck.

Furthermore, the side supporting plates are provided with operation openings corresponding to the bottom funnel necks and the positions below the bottom funnel necks, the number of the operation openings is 2, and the operation openings are symmetrically distributed along the side supporting plates. Preferably, the operation opening can be used for palm deep operation.

Further, a pair of side support rods are symmetrically fixed on the outer side wall of the bottom funnel neck perpendicular to the operation opening, and the end parts of the side support rods are fixed to the inner wall surfaces of the side support plates.

Furthermore, the outlet pipe communicated with the bottom of the separation cavity is a hose, the other side of the hose is connected with a hard pipe, and a plug is arranged in the hard pipe.

Furthermore, a reagent tube can be erected in the second sample adding hole.

Further, the upper end of the reagent tube is a tube cap, the tube cap is screwed with the tube body through threads, the diameter of the tube cap is larger than that of the second sampling hole, and the diameter of the tube body is smaller than that of the second sampling hole.

Furthermore, the middle part of the pipe cover is of a hollow structure, the top of the hollow structure is sealed by a sealing film, the bottom of the pipe body is a conical flexible pipe end, and an extrusion rod can be inserted into the hollow structure.

Furthermore, the top of the extrusion rod is a rod head, a pull ring is fixed on the rod head, the diameter of the rod head of the extrusion rod is larger than that of the tube body of the reagent tube, and an extrusion pointed end is arranged at the bottom of the rod body of the extrusion rod. Preferably, the diameter of the rod body is 1/5 ~ 1/4 of the pipe body, the length of the rod body is greater than the length of the pipe body, and preferably the length of the rod body is about 1.05 ~ 1.1 times the length of the pipe body.

Further, the specific structure of the extrusion tip is as follows: the long and short spikes are distributed at intervals.

By adopting the technical scheme, compared with the prior art, the utility model has the following technical effects:

according to the cord blood stem cell separation device, the piston is arranged to gradually separate and discharge cord blood, a complicated three-way pipe and a storage bag connected with a plurality of plastic pipes in the prior art are eliminated, the structure is simple, and the operation is convenient; the whole separating device is of a pipe column type structure, the existing centrifugal equipment can be adopted to carry out centrifugation on the separating device, the separating effect is good, and the applicability is strong; in addition, the required separation reagent can be stored in the reagent tube according to the preset specification in advance, so that the weighing process in the operation process is omitted, the operation time is saved, and the separation efficiency is improved.

Drawings

The accompanying drawings, which are included to provide a further understanding of the utility model and are incorporated in and constitute a part of this specification, illustrate embodiments of the utility model and together with the description serve to explain the utility model and not to limit the utility model. In the drawings:

FIG. 1 is a schematic structural diagram of a cord blood stem cell separation device in a preferred embodiment of the present invention.

FIG. 2 is a schematic cross-sectional view of a cord blood stem cell separation apparatus in accordance with a preferred embodiment of the present invention.

FIG. 3 is a schematic structural diagram of an assembled reagent tube of a cord blood stem cell separation device according to a preferred embodiment of the present invention; wherein, part A is the structural schematic diagram of the reagent tube, part B is the structural schematic diagram of the extrusion rod, and part C is the assembly schematic diagram of the reagent tube and the extrusion rod.

Wherein the reference numerals in the figures are: 1-a separation chamber; 2-covering the upper cover; 21-a first well; 22-a second well; 23-a filter chamber; 3-a support frame; 31-a bottom support plate; 32-side support plates; 4-a piston; 5-side support rods; 6-an outlet pipe; 7-plugging; 8-reagent tube; 81-tube cover; 82-a flexible tube end; 83-sealing film; 9-extruding a rod; 91-a club head; 92-an extrusion tip; 93-a pull ring; 10-an operating opening; 11-a bottom support bar; 12-waste port.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. Unless defined or indicated otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The components and their connections in the following examples are conventional in the art unless otherwise defined or indicated.

Reference in the specification to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the utility model. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one of ordinary skill in the art that the described embodiments of the present invention can be combined with other embodiments without conflict. The utility model is further described with reference to the following drawings and specific examples, which are not intended to be limiting.

Example 1

The present embodiment is a cord blood stem cell separation device with a better structure, as shown in fig. 1-2, the cord blood stem cell separation device includes a separation cavity 1 and an upper cover 2 for covering the separation cavity 1, the separation cavity 1 is a transparent funnel-type structure so as to facilitate observation of a cord blood stratification interface in a separation process, and the separation cavity 1 includes a conventional upper cylinder, a lower cone and a bottom funnel neck. The upper cover 2 is provided with a first sample adding hole 21 and a second sample adding hole 22 for adding the collected umbilical cord blood and hydroxyethyl starch respectively, and the umbilical cord blood is added by transferring with a conventional puncture needle. The first sample adding hole 21 is communicated with a filter cavity 23 positioned at the upper part of the separation cavity 1, the form and the structure of the filter cavity 23 are conventional in the field, for example, at least a filter membrane, an isolation net, a guide plate, a flow guide port, a human-shaped membrane and the like can be arranged in the filter cavity 23 to filter out aggregate particles, thereby being beneficial to separating the umbilical cord blood stem cells. The upper cover 2 can be clamped to the top of the separation cavity 1, a contact part is provided with a sealing strip, and the upper cover 2 is provided with a handle so as to realize the disassembly of the upper cover 2 and the separation cavity 1; corresponding sealing plugs are arranged in the first sample adding hole 21 and the second sample adding hole 22, and pull rings are arranged on the sealing plugs to remove the sealing plugs.

In this embodiment, the side of the separation chamber 1 is fixed to the support frame 3, the bottom surface of the support frame 3 is a cylindrical bottom support plate 31, the side of the support frame 3 is an annular side support plate 32, the bottom of the side support plate 32 is fixed to the edge of the bottom support plate 31, the bottom support plate 31 and the side support plate 32 can be of an integrally formed structure, the top of the side support plate 32 of the support frame 3 and the bottom of the upper cylinder of the separation chamber 1 can be directly bonded by a strong adhesive, the lower part of the separation chamber 1 is provided with a piston 4, specifically, a piston 4 is arranged in the bottom funnel neck of the separation chamber 1, a sealing ring is arranged between the upper end of the piston 4 and the bottom funnel neck, and can slide up and down in the bottom funnel neck, and the lower end of the piston 4 is an operable end and is located below the outside of the separation chamber 1. The bottom side of the above-mentioned separation chamber 1 is provided with an outlet pipe 6 to move the layered liquid into the storage bag in sequence, the bottom of the piston 4 is provided with a bottom support rod 11, and the fixed form of the bottom support rod 11 is as follows: set up first recess in the middle of the bottom of piston 4, set up the second recess in the middle of the top of end support plate 31, the upper end and the lower extreme of end support pole 11 correspond respectively to block and establish to first recess and second recess in, above-mentioned end support pole 11 preferred have certain elasticity in order to carry out steady and slow decline when adding the umbilical cord blood of treating in separation chamber 1 piston 4. When the bottom support rod 11 needs to be clamped or removed, the piston 4 can be manually pushed upwards. It will be appreciated that with the bottom support rod 11 supported or removed, the elevation of the top seal of the piston 4 is greater than the elevation of the outlet tube 6 to prevent unseparated liquid from exiting. The support frame 3 and the bottom support rod 11 can stably fix the separation cavity 1 under the condition of no operation or separation operation, the service life of the separation cavity is prolonged, the stability of the separation process can be improved, and the separation effect is effectively guaranteed.

In a preferred embodiment, the side supporting plate 32 is provided with 2 operation openings 10 corresponding to the bottom funnel neck of the separation chamber 1 and the lower position thereof, the operation openings 10 are symmetrically distributed along the side supporting plate 32, that is, the front and the rear are both provided with the operation openings 10, and the size of the operation openings 10 can be used for the palm to extend into the operation, because the bottom funnel neck is of a transparent structure, the layered interface can be easily observed in the separation process to realize effective separation; the outer side wall of the bottom funnel neck of the separation chamber 1 is symmetrically fixed with a pair of side support rods 5 at the position vertical to the operation opening 10, the end parts of the side support rods 5 are fixed to the inner wall surface of the side support plate 32, and the fixing mode can adopt the conventional screw connection. The side support bars 5 are provided to further support the separation chamber 1.

In a preferred embodiment, the outlet pipe 6 connected to the bottom of the separation chamber 1 is a flexible pipe, the flexible pipe and the separation chamber 1 can be integrally formed, the flexible pipe has a certain elasticity, and the other side of the flexible pipe can be sleeved with a hard pipe, and a plug 7 is arranged in the hard pipe when the separation operation is not performed, so as to achieve the sealing effect.

In a preferred embodiment, as shown in fig. 3 a-C, the reagent tube 8 can be disposed in the second sample adding hole 22, the upper end of the reagent tube 8 is a tube cap 81, the tube cap 81 is screwed to the tube body, the diameter of the tube cap 81 is larger than the diameter of the second sample adding hole 22, and the diameter of the tube body is smaller than the diameter of the second sample adding hole 22. The reagent tube 8 is stored with a predetermined amount of hydroxyethyl starch, when in operation, the reagent tube 8 can be put in by moving away the sealing plug of the second sample adding hole 22, after the addition of the umbilical cord blood is completed, the reagent tube 8 is taken out, the tube cover 81 is opened, and the hydroxyethyl starch in the reagent tube 8 is added into the separation cavity 1, so that the reagent tube 8 can be arranged to save the reagent measuring operation in the conventional separation, save the manual operation time and improve the working efficiency.

In a preferred embodiment, the reagent tube 8 has a hollow structure in the middle of the cap 83, the top of the hollow structure is sealed by a sealing film 83, and the bottom of the tube body is a tapered flexible tube end 82, into which the squeeze rod 9 can be inserted. The top of above-mentioned squeeze beam 9 is pole head 91, fixes pull ring 93 on pole head 91, and the pole head diameter of squeeze beam 9 is greater than the diameter of the 8 bodys of reagent pipe, and the bottom of the body of rod of squeeze beam 9 sets up extrusion tip 92, and extrusion tip 92's concrete structure is: the length prick interval distribution, 1/5 ~ 1/4 that the diameter of the above-mentioned body of rod is about the body diameter is in order to prevent overflowing of extrusion in-process reagent, the length of the body of rod is greater than the length of body, preferably 1.05 ~ 1.1 times of body length is in order to puncture the flexible pipe end 82 of toper of reagent pipe 8 smoothly in the extrusion process, thereby realize the joining of reagent, above-mentioned seal membrane 83 need tear when the operation and stretch into with the pinch bar 9, thereby the pollution of pollutant to the parting fluid can be avoided in taking out of reagent pipe 8 to the setting of above-mentioned reagent pipe 8 and pinch bar 9.

In a preferred embodiment, the bottom of the bottom funnel neck of the separation chamber 1 is provided with a waste liquid port 13 for discharging the separation liquid which may leak in an accidental situation.

The operation flow of the cord blood stem cell separation device in the above preferred embodiment is briefly as follows: closing the upper cover 2 to the separation cavity 1, opening sealing plugs of the first sample adding hole 21 and the second sample adding hole 22, moving the reagent tube 8 to the second sample adding hole 22, adding the umbilical cord blood to the filter cavity 23 by adopting a puncture needle, allowing the filtered umbilical cord blood to fall into the separation cavity 1, adding hydroxyethyl starch into the separation cavity 1 through corresponding operation of the reagent tube 8, sealing the first sample adding hole 21 and the second sample adding hole 22 by using the sealing plugs, standing for 30 minutes to allow red blood cells to naturally settle, removing the plug 7 on the outlet tube 6 at the moment, communicating the plug 7 to a first storage bag, removing the bottom support rod 11, transferring the red blood cells on the lower layer to the first storage bag through manual operation, and removing the first storage bag. After the red blood cells are separated, the bottom support rod 11 is fixed again, and the outlet pipe is sealed again by the plug 7. And (3) placing the separation device into a centrifuge for centrifugation, wherein the centrifugation condition is a conventional operation process, and after the centrifugation is finished, the umbilical cord blood is divided into an upper plasma layer and a lower umbilical cord blood hematopoietic stem cell. At this time, the stopper 7 on the outlet tube 6 is removed and communicated to the second storage bag, the bottom support rod 11 is removed, the cord blood hematopoietic stem cells in the lower layer are transferred to the second storage bag by manual operation, and the second storage bag is removed. Re-fixing the bottom support rod 11, connecting the outlet tube 6 to the third storage bag, removing the bottom support rod 11, transferring the remaining plasma layer to the third storage bag by manual operation, and removing the third storage bag. The separated umbilical cord blood hematopoietic stem cells, red blood cells and blood plasma are stored separately.

According to the embodiment, the umbilical cord blood stem cell separation device is provided with the piston type separation mode to separate umbilical cord blood hematopoietic stem cells, red blood cells and blood plasma, a complicated three-way pipe and a storage bag connected by a plurality of plastic pipes in the prior art are abandoned, and the umbilical cord blood stem cell separation device is simple in structure and convenient to operate; the whole separating device is of a pipe column type structure, the existing centrifugal equipment can be adopted to carry out centrifugation on the separating device, the separating effect is good, and the applicability is strong; and the required separation reagent can be stored in the reagent tube according to the preset specification in advance, so that the weighing process in the operation process is omitted, the operation time is saved, and the separation efficiency is improved.

The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the utility model. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (10)

1. The cord blood stem cell separation device comprises a separation cavity (1) and an upper cover (2) used for covering the separation cavity (1), wherein a first sample adding hole (21) and a second sample adding hole (22) are formed in the upper cover (2), the first sample adding hole (21) is communicated with a filter cavity (23) located on the upper portion of the separation cavity (1), the cord blood stem cell separation device is characterized in that the side surface of the separation cavity (1) is fixed to a support frame (3), a piston (4) is arranged on the lower portion of the separation cavity (1), an outlet pipe (6) is arranged on the side surface of the bottom of the separation cavity (1), and a bottom support rod (11) is arranged at the bottom of the piston (4); the bottom surface of the support frame (3) is a cylindrical bottom support plate (31), the side surface of the support frame (3) is an annular side support plate (32), and the bottom of the side support plate (32) is fixed to the edge of the bottom support plate (31).

2. The cord blood stem cell separation device according to claim 1, wherein a first groove is arranged in the middle of the bottom of the piston (4), a second groove is arranged in the middle of the top of the bottom support plate (31), and the upper end and the lower end of the bottom support rod (11) are correspondingly clamped into the first groove and the second groove respectively.

3. The cord blood stem cell separation device according to claim 1, wherein the separation chamber (1) is a transparent funnel-shaped structure comprising an upper cylinder, a lower cone and a bottom funnel neck, the bottom of the upper cylinder is fixed with the top of the side support plate (32), and a piston (4) is arranged in the bottom funnel neck.

4. A cord blood stem cell separation device according to claim 3, wherein operation openings (10) are opened at the side support plate (32) corresponding to the bottom funnel neck and the lower position thereof, the number of the operation openings (10) is 2, which are symmetrically distributed along the side support plate (32).

5. An umbilical cord blood stem cell separation device as claimed in claim 4, wherein the outer side wall of the bottom funnel neck symmetrically fixes a pair of side support rods (5) at the position perpendicular to the operation opening (10), the ends of the side support rods (5) are fixed to the inner wall surface of the side support plate (32).

6. The cord blood stem cell separation device according to claim 1, wherein the outlet pipe (6) communicated with the bottom of the separation chamber (1) is a flexible pipe, and the other side of the flexible pipe is connected with a hard pipe, and a plug (7) is arranged in the hard pipe.

7. The cord blood stem cell separation device according to claim 1, wherein a reagent tube (8) can be mounted in the second sample application hole (22).

8. The cord blood stem cell separation device according to claim 7, wherein the upper end of the reagent tube (8) is a tube cap (81), the tube cap (81) is screwed with a tube body, the diameter of the tube cap (81) is larger than the diameter of the second sample adding hole (22), and the diameter of the tube body is smaller than the diameter of the second sample adding hole (22).

9. The cord blood stem cell separation device according to claim 8, wherein the middle part of the tube cover (81) is a hollow structure, the top of the hollow structure is sealed by a sealing film (83), the bottom of the tube body is a tapered flexible tube end (82), and the extrusion rod (9) can be inserted into the hollow structure.

10. The cord blood stem cell separation device according to claim 9, wherein the top of the extrusion rod (9) is a rod head (91), a pull ring (93) is fixed on the rod head (91), the diameter of the rod head of the extrusion rod (9) is larger than that of the tube body of the reagent tube (8), and the bottom of the rod body of the extrusion rod (9) is provided with an extrusion pointed tip (92).

Images