CN207685278U - Menstrual blood inner membrance stem cell quick disconnector - Google Patents
Menstrual blood inner membrance stem cell quick disconnector Download PDFInfo
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- CN207685278U CN207685278U CN201721728970.9U CN201721728970U CN207685278U CN 207685278 U CN207685278 U CN 207685278U CN 201721728970 U CN201721728970 U CN 201721728970U CN 207685278 U CN207685278 U CN 207685278U
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Abstract
The utility model discloses a kind of menstrual blood inner membrance stem cell quick disconnectors,Including pipe lid,Tube body,Digest tube and withdrawal device,Tube body is made of 4 dismountable pipe shafts screw socket rotatable connection,It is furnished with gasket in screw socket junction,4 pipe shafts are respectively top layer pipe shaft,Pipe shaft among first layer,Pipe shaft and bottom pipe shaft among the second layer,Top layer pipe shaft,Pipe shaft among first layer,It is each in pipe shaft among the second layer that there are one strainers,It is 40 μm of strainers built in top layer pipe shaft,It is 10 μm of strainers built in the pipe shaft of first layer centre,It is 0.3 μm of strainer built in the pipe shaft of second layer centre,The bottom end of bottom pipe shaft is taper,Conical lower portion carries through-hole,Withdrawal device is connected to by connecting tube on through-hole,The screw socket of the digest tube of separate configurations is inside and outside double screw sockets,It is sealed by closed bottle cap,Inside and outside double screw sockets can be combined with the screw socket on any section pipe shaft of tube body.The utility model uses simplicity, easy to operate, can greatly improve the separative efficiency of menstrual blood inner membrance stem cell, and ensure that separation operation process is pollution-free.
Description
Technical field
The utility model is related to a kind of separator, especially a kind of menstrual blood inner membrance stem cell quick disconnector.
Background technology
Endometrial stem cell is a kind of adult stem cell, the potential with self-renewing, Multidirectional Differentiation and hyperproliferation.
Numerous studies have proven in endometrium there are stem cell-like cell at present, to make endometrium have very strong Regenerated energy
Power；Meanwhile research also shows that the function of Endometrial stem cell and living environment extremely may be with endometriosis, uterus
The gynecological diseases such as endometrial carcinomas are related.Although the biological characteristics and function of Endometrial stem cell still need to further study to explain
It states and confirms, but the regeneration of its endometrium and be divided into the potential of its hetero-organization and got growing concern for, and may be at
The means that inner membrance repairs replacement therapy are carried out with intimal lesion patient for endometrium is excessively thin；Can be simultaneously mullerianosis
The new Research Thinking of the offers such as disease, carcinoma of endometrium.
Containing the endometrium that largely falls off in menstrual blood, therefore separating uterus inner membrance and stem cell is detached from menstrual blood
It is a convenience and noninvasive method, therefore the efficiency for how improving the segregant endometrial stem cells from menstrual blood is got over
Carry out more attention.
Utility model content
Technical problem to be solved by the utility model is to provide a kind of menstrual blood inner membrance stem cell quick disconnector, letters
Easy operation not only can efficiently separate out the stem cell in menstrual blood inner membrance, at the same can also be fallen by membrane filtration in blood it is red,
The isolated autologous plasma of leucocyte is used for the culture of menstrual blood inner membrance stem cell, and quick separating goes out the son in menstrual blood
Endometrium simultaneously quickly carries out digestion acquisition stem cell.
In order to solve the above-mentioned technical problem, the technical solution adopted in the utility model is：A kind of menstrual blood inner membrance stem cell
Quick disconnector, including pipe lid, tube body, digest tube and withdrawal device, tube body are rotatably connected group by 4 dismountable pipe shafts by screw socket
Be furnished with gasket at, screw socket junction, 4 pipe shafts are respectively top layer pipe shaft, pipe shaft among first layer, among the second layer pipe shaft and
Bottom pipe shaft, it is top layer pipe shaft, pipe shaft among first layer, each there are one strainer in pipe shaft among the second layer, built in top layer pipe shaft
It is 10 μm of strainers built in pipe shaft for 40 μm of strainers, among first layer, built in pipe shaft is 0.3 μm of strainer, bottom among the second layer
The bottom end of layer pipe shaft is taper, and conical lower portion carries through-hole, and withdrawal device is connected to by connecting tube on through-hole, and separate configurations disappear
The screw socket for changing pipe is inside and outside double screw sockets, is sealed by closed bottle cap, and inside and outside double screw sockets can be with the screw socket on any section pipe shaft of tube body
It is combined.
The pipe lid and tube body are rotated or are snapped connection by screw socket.
The withdrawal device is disposable syringe.
The utility model has the beneficial effects that：It is easy to operate using simplicity, point of menstrual blood inner membrance stem cell can be greatly improved
From efficiency, and it ensure that separation operation process is pollution-free.
Description of the drawings
Fig. 1 is the structural schematic diagram of the utility model menstrual blood inner membrance stem cell quick disconnector.
Specific implementation mode
The utility model is described in further detail with reference to the accompanying drawings and detailed description：
As shown in Figure 1, the menstrual blood inner membrance stem cell quick disconnector of the utility model, including pipe lid 1, tube body 2, digestion
Pipe 6 and withdrawal device 4, tube body 2 by 4 dismountable pipe shafts by screw socket rotatable connection form, screw socket junction be furnished with gasket, 4
A pipe shaft is respectively top layer pipe shaft 2A, first layer centre pipe shaft 2B, second layer centre pipe shaft 2C and bottom pipe shaft 2D, top layer pipe shaft
It is 2A, pipe shaft 2B among first layer, each there are one strainer 3A, 3B, 3C in pipe shaft 2C among the second layer, be built in top layer pipe shaft 2A
It is 10 μm of strainer 3B built in 40 μm of strainer 3A, first layer centre pipe shaft 2B, is 0.3 μm of filter built in the pipe shaft 2C of second layer centre
3C is netted, the bottom end of bottom pipe shaft 2D is taper, and conical lower portion carries through-hole 7, and withdrawal device 4 is connected to through-hole 7 by connecting tube 5
On, the screw socket of the digest tube 6 of separate configurations is inside and outside double screw sockets, is sealed by closed bottle cap, inside and outside double screw sockets can appoint with tube body 2
Screw socket on one section pipe shaft is combined.
The pipe lid 1 and tube body 2 are rotated or are snapped connection by screw socket.
The withdrawal device 4 is disposable syringe..
The principle of the utility model menstrual blood inner membrance stem cell quick disconnector：Due in menstrual blood containing largely falling off
Endometrium, and endometrial tissue and stem cell, red blood cell and the leucocyte being detached from is of different sizes, is filtered using different pore size
Film carries out Selective Separation by the method for extraction to sample, obtains Endometrial stem cell in menstrual blood, red blood cell and white
Cell, blood plasma.
Above-mentioned menstruation hemocytoblast separator application method is as follows：
Step 1：The pipe lid of separator is opened in clean bench, pipe shaft is put on bracket.
Step 2：Menstrual blood is added in separator, separator can detach total blood volume≤15ml every time, tighten pipe
Lid.
Step 3：The pull rod for twitching withdrawal device, makes menstrual blood have stepped through 3 strainers, until menstrual blood has all filtered,
It is the whole blood plasma extracted in withdrawal device 4, unplugs withdrawal device and the connecting portion of connecting tube, blood plasma is transferred to and is preserved in pipe
It preserves.
Step 4：First screw socket is unscrewed, the strainer 2A containing inner membrance fragment is combined with digest tube and is screwed, and from nozzle
Digestive juice is added to not having filter screen 2A, is digested, after digestion fully carries out that stem cell is collected by centrifugation.
Step 5：The strainer 3B among first layer built in pipe shaft 2B is purged repeatedly with sterile 1ml pipette tips absorption 1mlPBS, it will
Suspension containing stem cell is put into stem cell and is mixed with the stem cell collected in step 4, is cultivated.
Step 6：If blood volume to be separated is more than 15ml, needs the tube body group of separator to load onto and open again
Begin to operate, operating procedure is same as above (step 1~step 6).
In conclusion the content of the utility model is not limited in the above embodiments, in same area it is insighted it
Scholar can easily propose other embodiments within the technological guidance's thought of the utility model, but this embodiment is all wrapped
It includes within the scope of the utility model.
Claims (3)
1. a kind of menstrual blood inner membrance stem cell quick disconnector, including pipe lid (1), tube body (2), digest tube (6) and withdrawal device
(4), which is characterized in that tube body (2) is made of 4 dismountable pipe shafts screw socket rotatable connection, and screw socket junction is equipped with sealing
Pad, 4 pipe shafts are respectively top layer pipe shaft (2A), first layer centre pipe shaft (2B), second layer centre pipe shaft (2C) and bottom pipe shaft
(2D), top layer pipe shaft (2A), pipe shaft (2B) among first layer, among the second layer in pipe shaft (2C) it is each there are one strainer (3A, 3B,
3C), built in top layer pipe shaft (2A) it is 40 μm of strainers (3A), is 10 μm of strainers (3B) built in first layer centre pipe shaft (2B),
It is 0.3 μm of strainer (3C) built in pipe shaft (2C) among the second layer, the bottom end of bottom pipe shaft (2D) is taper, and conical lower portion carries
Through-hole (7), withdrawal device (4) are connected to by connecting tube (5) on through-hole (7), and the screw socket of the digest tube (6) of separate configurations is inside and outside
Double screw sockets, are sealed by closed bottle cap, and inside and outside double screw sockets can be combined with the screw socket on any section pipe shaft of tube body (2).
2. menstrual blood inner membrance stem cell quick disconnector according to claim 1, which is characterized in that the pipe lid (1) and pipe
Body (2) is rotated or is snapped connection by screw socket.
3. menstrual blood inner membrance stem cell quick disconnector according to claim 1, which is characterized in that the withdrawal device (4) is
Disposable syringe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721728970.9U CN207685278U (en) | 2017-12-12 | 2017-12-12 | Menstrual blood inner membrance stem cell quick disconnector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721728970.9U CN207685278U (en) | 2017-12-12 | 2017-12-12 | Menstrual blood inner membrance stem cell quick disconnector |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207685278U true CN207685278U (en) | 2018-08-03 |
Family
ID=62997569
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201721728970.9U Active CN207685278U (en) | 2017-12-12 | 2017-12-12 | Menstrual blood inner membrance stem cell quick disconnector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN207685278U (en) |
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2017
- 2017-12-12 CN CN201721728970.9U patent/CN207685278U/en active Active
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