CN114011245A - Cell filter and filtering method thereof - Google Patents

Abstract

The invention relates to a cell filter and a filtering method thereof, relating to the technical field of medical and laboratory instruments, comprising an upper collector which is cylindrical in shape and a filtering area which is integrally formed with the lower end of the upper collector, wherein the upper end of the upper collector is provided with a cover, a valve is arranged at the communication part of the upper collector and the filtering area, and the bottom of the filtering area is provided with a filter membrane; still include down the collector, down the collector with filter zone lower extreme can dismantle the connection. The structure of the cell filter enables the blowing, the filtering and the collecting to be completed at one time, the operation is simple and convenient, the possibility of pollution is reduced, the sterile requirement is better met, the simplicity and the strict sterile design of the operation are realized, and more convenient experimental conditions can be provided for experimenters.

Description

Cell filter and filtering method thereof

Technical Field

The invention relates to the technical field of medical and laboratory instruments, in particular to a cell filter and a filtering method thereof.

Background

Cell primary culture, which is a culture stage in which cells, tissues and organs are directly obtained from an organism and cultured until passage, is one of the most commonly used means for biological and medical research. The method can provide powerful means for researching the growth, metabolism and propagation of organism cells, and simultaneously creates conditions for the subsequent subculture, and can directly serve the clinical practice. After the tissues are separated and digested in the animal body, original tissues, organs, cell clusters and culture solution which are not completely dispersed are generally provided in addition to primary cells, so that the mixed solution needs to be blown and beaten firstly and then preliminarily filtered in the collection of products, a cell filter needs to be used for filtering the mixed solution, and the cell filter can complete impurity filtration, cell dispersion, sample separation and the like.

The cell filter provides a simple and feasible method for the paper cup single cell suspension. The existing cell filter filters through a filter screen with a specific pore diameter, wherein a disposable cell filter screen/a cell filter screen made of a metal material is sleeved on a centrifuge tube, a cell suspension liquid blown by a suction tube is sucked and dropped on the filter screen for filtering, and then the filtered cell suspension liquid is sucked, collected and inoculated into a culture dish.

The steps are relatively separated, the operation is complex, and the requirement on sterile operation of laboratory personnel is high; moreover, the metal cell filter screen used in the filtering process is repeatedly used after being sterilized under high pressure, and is easy to rust after being used for a long time, so that pollution is caused; for the cell filtering screen made of metal material, the prior art also has the steps of cleaning with dilute alkaline water to remove the residual cells and tissues on the filtering screen, then soaking and cleaning with a detergent solution, finally washing and cleaning the filtering screen with tap water, washing with distilled water and redistilled water, sterilizing the filtering screen and the package, and then putting the filtering screen into the package for preservation.

Disclosure of Invention

The invention aims to provide a cell filter and a filtering method thereof, which are used for solving the technical problems in the prior art, so that the blowing, filtering and collecting can be completed at one time, the operation is simple and convenient, the pollution possibility is reduced, and the sterile requirement is better met.

In order to achieve the purpose, the invention provides the following scheme:

the invention discloses a cell filter, comprising:

the upper end and the lower end of the upper collector are opened;

the filtering area is fixed at the lower end of the upper collector, and a filtering membrane is arranged at the bottom of the filtering area;

a cover detachably coupled to an upper end of the upper collector;

the valve is arranged at the communication position of the upper collector and the filtering area;

the lower collector is detachably connected with the lower end of the filtering area.

Preferably, the inner side of the cover is provided with internal threads, the outer surface of the upper end of the upper collector is provided with external threads, and the cover is in threaded connection with the upper collector.

Preferably, the lower end of the lower collector and the lower end of the filtering area are in threaded connection, plug seal or interference fit.

Preferably, the lower collector is cylindrical in shape.

Preferably, the filter membrane adopts a nylon screen mesh structure.

Preferably, the valve is a medical check valve.

Preferably, the filtering area is in the shape of a cylinder or a cone.

The invention also discloses a filtering method of the cell filter, which comprises the following steps:

s1, unscrewing a cover, and flushing the mixed solution into an upper collector by using a syringe;

s2, uniformly blowing and beating the cells in the mixed solution in an upper collector by using a disposable dropper;

s3, opening a valve, enabling the mixed solution to enter a filtering area, filtering by a filter membrane and flowing to a lower collector;

s4, unscrewing the collector, sucking the filtered mixed solution, and planting the mixed solution into a culture bottle.

Compared with the prior art, the invention has the following technical effects:

by adopting the technical scheme, the upper collector is more convenient than a culture dish and is not easy to overflow in the cell blowing process, and compared with a 50ml centrifugal tube, the upper collector removes redundant length and saves materials; the sealing performance of the intermediate valve ensures the sterility and the up-and-down separation of the interior of the device; the diameters of the upper collector and the lower collector are the same as those of a 50ml centrifuge tube, and when the cell filter is used, the cell filter can be directly inserted into a test tube rack, so that the operation is convenient; the filter area and the lower collector are detachably connected, so that the sterility in the cell filter is ensured, the lower collector can be conveniently taken out, and the filtered liquid can be conveniently collected. The structure of the cell filter enables the blowing, the filtering and the collecting to be completed at one time, the operation is simple and convenient, the possibility of pollution is reduced, the sterile requirement is better met, the simplicity and the strict sterile design of the operation are realized, and more convenient experimental conditions can be provided for experimenters.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

FIG. 1 is a schematic view of a cell filter according to the present invention;

FIG. 2 is a cross-sectional view of the cell filter of the present invention.

In the figure: 1. a cover; 2. an upper collector; 3. a valve; 4. filtering the membrane; 5. a lower collector; 6. a filtration zone.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

The invention aims to provide a cell filter and a filtering method thereof, which are used for solving the technical problems in the prior art, so that the blowing, filtering and collecting can be completed at one time, the operation is simple and convenient, the pollution possibility is reduced, and the sterile requirement is better met.

In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with figures are described in further detail below.

First embodiment, as shown in fig. 1 and 2, a cell filter comprises an upper collector 2 in the shape of a cylinder and a filtering area 6 integrally formed with the lower end of the upper collector 2, the cylinder has a central passage for liquid to flow through, the upper end of the upper collector 2 is provided with a cover 1, a valve 3 is arranged at the communication part of the upper collector 2 and the filtering area 6, and the bottom of the filtering area 6 is provided with a filter membrane 4; the filter further comprises a lower collector 5, and the lower collector 5 is detachably connected with the lower end of the filter area 6.

The second embodiment discloses a cell filter, which comprises an upper collector 2 and a filtering area 6, wherein the upper collector 2 is in a cylindrical shape, the filtering area 6 is integrally formed with the lower end of the upper collector 2, the cylindrical shape is provided with a central channel through which liquid can flow, the upper end of the upper collector 2 is provided with a cover 1, a valve 3 is arranged at the communication part of the upper collector 2 and the filtering area 6, and the bottom of the filtering area 6 is provided with a filter membrane 4;

the filter further comprises a lower collector 5, and the lower collector 5 is detachably connected with the lower end of the filter area 6. The inner thread of the inner side of the cover 1 is connected with the outer thread of the outer surface of one end of the upper collector 2 through screw threads. The connection mode of the filtering area 6 and the lower collector 5 is threaded connection, plug seal or interference fit, namely, the external thread of the outer side surface of the lower end of the filtering area 6 is connected with the internal thread of the inner side surface of the upper end of the lower collector 5, or the lower end of the filtering area 6 is plugged into the upper end of the lower collector 5 in a sealing mode, or the interference fit is adopted, and the upper end of the lower collector 5 is sleeved to the lower end of the filtering area 6 by utilizing the characteristics of thermal expansion and cold contraction.

In this embodiment, the lower collector 5 is in the shape of a cylinder having a central passage through which the liquid can flow.

It should be noted that the diameter of the upper collector 2 and the lower collector 5 is equal to the diameter of a 50ml centrifuge tube, and the length of the upper collector 2 is less than the length of the 50ml centrifuge tube.

It should be noted that the filter membrane 4 is of a nylon mesh structure, and the cell filter is made of plastic except for the filter membrane 4.

It will be appreciated that the valve 3 is a medical check valve.

In addition, the filtering area 6 is cylindrical or conical in shape.

When in actual use, firstly the cover 1 is unscrewed, the marrow fluid is washed into the upper collector 2 by a syringe, the bone marrow cells are blown uniformly by a disposable dropper in the upper collector 2, then the valve 3 is opened, the cell suspension enters the filtering area 6, flows into the lower collector 5 after being filtered by the filter membrane 4, the lower collector 5 is unscrewed, and the filtered single-cell suspension is sucked and planted into a culture bottle/culture dish. Therefore, the cell filter can complete blowing, filtering and collecting at one time, is simple and convenient to operate, reduces the possibility of pollution and better meets the aseptic requirement.

By adopting the technical scheme, the upper collector is more convenient than a culture dish and is not easy to overflow in the cell blowing process, and compared with a 50ml centrifugal tube, the upper collector removes redundant length and saves materials; the sealing performance of the intermediate valve ensures the sterility and the up-and-down separation of the interior of the device; the filter area and the lower collector are detachably connected, so that the sterility in the cell filter is ensured, the lower collector can be conveniently taken out, and the filtered liquid can be conveniently collected. The structure of the cell filter enables the blowing, the filtering and the collecting to be completed at one time, the operation is simple and convenient, the possibility of pollution is reduced, the sterile requirement is better met, the simplicity and the strict sterile design of the operation are realized, and more convenient experimental conditions can be provided for experimenters.

The principle and the implementation mode of the present invention are explained by applying specific examples in the present specification, and the above descriptions of the examples are only used to help understanding the method and the core idea of the present invention; meanwhile, for a person skilled in the art, according to the idea of the present invention, the specific embodiments and the application range may be changed. In view of the above, the present disclosure should not be construed as limiting the invention.

Claims (8)

1. A cell filter, comprising:

the upper end and the lower end of the upper collector are opened;

the filtering area is fixed at the lower end of the upper collector, and a filtering membrane is arranged at the bottom of the filtering area;

a cover detachably coupled to an upper end of the upper collector;

the valve is arranged at the communication position of the upper collector and the filtering area;

the lower collector is detachably connected with the lower end of the filtering area.

2. The cell filter of claim 1, wherein: the inner side of the cover is provided with internal threads, the outer surface of the upper end of the upper collector is provided with external threads, and the cover is connected with the upper collector through threads.

3. The cell filter of claim 1, wherein: the lower end of the lower collector and the lower end of the filtering area are in threaded connection, plug seal or interference fit.

4. The cell filter of claim 1, wherein: the lower collector is cylindrical in shape.

5. The cell filter of claim 1, wherein: the filter membrane adopts a nylon screen mesh structure.

6. The cell filter of claim 1, wherein: the valve is a medical one-way valve.

7. The cell filter of claim 1, wherein: the filtering area is cylindrical or conical.

8. A method of filtration based on a cell filter according to any one of claims 1 to 7, comprising the steps of:

s1, unscrewing a cover, and flushing the mixed solution into an upper collector by using a syringe;

s2, uniformly blowing the cells in the mixed solution in an upper collector by using a disposable dropper;

s3, opening a valve, enabling the mixed solution to enter a filtering area, filtering by a filter membrane and flowing to a lower collector;

s4, unscrewing the collector, sucking the filtered mixed solution, and planting the mixed solution into a culture bottle.

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