CN205275584U - Obtain intestinal microbial community's extraction system in human excrement and urine of follow - Google Patents
Obtain intestinal microbial community's extraction system in human excrement and urine of follow Download PDFInfo
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- CN205275584U CN205275584U CN201520959785.5U CN201520959785U CN205275584U CN 205275584 U CN205275584 U CN 205275584U CN 201520959785 U CN201520959785 U CN 201520959785U CN 205275584 U CN205275584 U CN 205275584U
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Abstract
The utility model relates to an obtain intestinal microbial community's extraction system in human excrement and urine of follow. It is including sampling bag (14), homogeneity filter equipment (10) and centrifuge (11), filter bag (7) and homogenizer (13) are crossed including the homogeneity in homogeneity filter equipment (10), the inside that filter bag (7) were crossed to the homogeneity has filter membrane intermediate layer (8). The utility model discloses the configuration is reasonable, health and environmental, does benefit to high efficiency ground separation and extraction intestinal fungus crowd liquid, also does benefit to the product quality that promotes intestinal fungus crowd liquid.
Description
Technical field
The utility model relates to microorganism separation and extraction technology field, particularly relates to a kind of extraction system obtaining enteric microorganism bacterium group from human excrement and urine.
Background technology
Adopting intestinal microflora liquid to treat the disease that alteration of intestinal flora causes, its curative effect is better than antibiotic therapy, and can cure the disease that some microbiotic cannot cure, and has caused in recent years and has paid attention to widely both at home and abroad. Employing will from human excrement and urine separation and Extraction intestinal microflora overall, or be separated wherein probiotics and, for the mode of alteration of intestinal flora patient, occupy more and more important status with the advantage that antibiotic therapy is incomparable by future.
At present, in the kind that center of gravity has been placed on intestinal microflora clinical treating disease by investigators and curative effect, and prepared by the separation and Extraction for intestinal microflora, but simple and crude facilities and equipment is often adopted, such as, common plastics casing, plastics bag is adopted to sample, with common clarifixator homogeneous ight soil, then centrifugal or screen filtration etc. The facilities and equipment that adopts these simple and crude and the intestinal microflora liquid impurity prepared and pigment is many, sense organ is poor; Use open vessel are easily contaminated and crossed contamination, the strong negative that its operation element also brings sense organ and psychology to producer impacts and causes discomfort. Therefore, adopt simple and crude facilities and equipment to carry out separation and Extraction and prepare intestinal microflora liquid, no matter be its operating process, or make the quality aspect of product, the application of the person that seriously have impact on medical work and patient, to the acceptance of this project, hinder and have delayed the application clinically of this technology.
Practical novel content
The purpose of this utility model is to provide a kind of extraction system obtaining enteric microorganism bacterium group from human excrement and urine, the configuration of this extraction system is reasonable, hygienic environment-protecting, it is beneficial to separation and Extraction intestinal microflora liquid quickly and efficiently, also it is beneficial to the product quality promoting intestinal microflora liquid.
The purpose of this utility model is achieved through the following technical solutions:
A kind of extraction system obtaining enteric microorganism bacterium group from human excrement and urine, it is characterised in that: it includes sampler bag, uniform filtration device and whizzer; Described uniform filtration device comprises uniform filtration bag and homogenizer; There is filter membrane interlayer the inside of described uniform filtration bag.
For effectively removing the pigment in ight soil, impurity and peculiar smell further, the filter membrane hole pore size on above-mentioned filter membrane interlayer is 0.1mm��0.45mm.
As clear and definite further again, the two ends of above-mentioned filter membrane interlayer and low side are all fixed on the bag of above-mentioned uniform filtration bag.
For ensureing filtration result further, removing in parting liquid the parasitic ovum that may exist, the above-mentioned extraction system obtaining enteric microorganism bacterium group from human excrement and urine, also includes strainer. Above-mentioned strainer is chosen as stage filter, and wherein filtering net aperture from big to small within the scope of 0.2mm��0.04mm arrange. By, before and after the operation of washing centrifugation or in process, adopting strainer to carry out aseptic classified filtering, it is achieved that the parasitic ovum that may exist in filtering intestinal microflora liquid.
As clear and definite further again, above-mentioned homogenizer is chosen as Patting type homogenizer.
For being convenient to quick sampling further, reducing the pollution in sampling process and loss, above-mentioned sampler bag is chosen as two-layer sampling bag simultaneously; Described two-layer sampling bag is inside and outside two layers, and its bag top is provided with tearing notch; The bottom of described internal layer upwards folds, and bottom is fixed on the side outer wall of internal layer; The bottom of described internal layer is provided with a tearing notch.
As further preferably, the bag top of above-mentioned two-layer sampling bag is provided with sealing strip.
As more further preferably, the bag top of above-mentioned two-layer sampling bag is also provided with tear opening. This tear opening is positioned at the top of above-mentioned sealing strip.
The utility model has following useful effect:
(1), the utility model provide a kind of extraction system obtaining enteric microorganism bacterium group from human excrement and urine, this system configuration rationally, hygienic environment-protecting, separation and Extraction intestinal microflora liquid, is also beneficial to the product quality promoting intestinal microflora liquid quickly and efficiently;
(2), the two-layer sampling bag that adopts in this system, its reasonable in design, avoids pollution and the loss of sample in sampling process, simplifies sampling operation, avoid environmental pollution, greatly reduces the uncomfortable sense of operator's processing sample;
(3), this system combines employing uniform filtration bag and Patting type homogenizer, complete homogeneous and filtration procedure once, both effectively prevent in open clarifixator homogeneous, in open screen cloth, filter the pollution caused, and use the crossed contamination of sample room that common container causes, in turn simplify operating process.
Accompanying drawing explanation
Fig. 1 is the schematic diagram of the extraction system obtaining enteric microorganism bacterium group from human excrement and urine described in embodiment 1.
Fig. 2 is the structural representation of two-layer sampling bag in the extraction system obtaining enteric microorganism bacterium group from human excrement and urine described in Fig. 1.
Fig. 3 be two-layer sampling bag described in Fig. 1 tear skin off and through launch after structural representation.
Fig. 4 is the structural representation of uniform filtration bag in the extraction system obtaining enteric microorganism bacterium group from human excrement and urine described in Fig. 1.
Fig. 5 is the vertical view of uniform filtration bag described in Fig. 4.
Fig. 6 is the structural representation of two-layer sampling bag in the extraction system obtaining enteric microorganism bacterium group from human excrement and urine described in embodiment 2.
Fig. 7 is that two-layer sampling bag described in Fig. 6 removes outer and after launching structural representation.
Embodiment
It is below the utility model preferred embodiment, and it is not intended to limit protection domain of the present utility model.
Embodiment 1
As Figure 1-5, a kind of extraction system obtaining enteric microorganism bacterium group from human excrement and urine, it includes sampler bag 14, uniform filtration device 10, whizzer 11 and stage filter 12; Its sampler bag 14 adopts two-layer sampling bag 1; Its uniform filtration device 10 is made up of uniform filtration bag 7 and Patting type homogenizer 13; Its two-layer sampling bag 1 is inside and outside two layer 2,3, and bag top is provided with a tearing notch 4; Its internal layer 2 is set to the Rotary-table that upper surface area is greater than lower surface area, and the bottom of this internal layer 2 upwards folds, and bottom 9 is fixed on the side outer wall of internal layer 2; The bottom of its internal layer 2 is also provided with a tearing notch 4; The bag top of this two-layer sampling bag 1 is provided with sealing strip 5 and tear opening 6, and tear opening 6 is positioned at the top of sealing strip 5; There is a filter membrane interlayer 8 its uniform filtration bag 7 inside, and bag mouth place is provided with sealing strip 5; The two ends of this filter membrane interlayer 8 and low side are all fixed on the bag of uniform filtration bag 7, and the filter membrane hole pore size on filter membrane interlayer 8 is 0.4mm; In its stage filter, the aperture of filtering net is arranged from big to small between 0.2mm��0.04mm scope.
During embody rule, first adopt sterilizing two-layer sampling bag 1 special described in this example to sample, before its use, tear tear opening 6, realize sealing by sealing strip 5 after sampling, under cold storage environment, sample is sent to laboratory; After the tearing notch 4 on two-layer sampling bag 1 torn abandon outer bag 3, internal layer 2 is put into filter membrane interlayer 8 side of aseptic uniform filtration bag 7, tear the tearing notch 4 on internal layer 2 bottom, faecal samples directly enters uniform filtration bag 7 from the passage that tearing notch 4 is formed, and this step avoids the splashing of sample and adheres to loss; Add diluent, then realize patting uniform filtration by Patting type homogenizer 13, after sample comminution uniform filtration, obtained uniform filtration liquid; Afterwards again by the centrifugation washing in whizzer 11 of uniform filtration liquid, intestinal microflora liquid can be obtained, finally by stage filter 12 classified filtering, the parasitic ovum that may exist in filtering intestinal microflora liquid, centrifugal and filtration hockets, i.e. obtained product, afterwards again through aseptic subpackaged.
In its centrifugation washing step, abandoned in centrifuge tube as clear as crystal simultaneously, and observe supernatant liquor (being mainly water-soluble impurity) and lower floor's solid impurity of the few bacterium in every visual field under the microscope, and will retain in the many middle layer bacterium layers to counting of basis of microscopic observation every visual field bacterium after white and dilution 10 times, both separation and Extraction intestinal microflora efficiently, also effectively removes the pigment in ight soil, impurity, peculiar smell, reduce viscosity and the granularity of intestinal microflora liquid, decrease the pigment of intestinal microflora liquid because of ight soil, impurity and stink and the uncomfortable sense that causes, its separation and Extraction is effective, improve product quality.
Embodiment 2
Obtaining the extraction system of enteric microorganism bacterium group from human excrement and urine, it is made up of sampler bag 14, uniform filtration device 10, whizzer 11 and stage filter 12; Its sampler bag 14 adopts two-layer sampling bag 1; Its uniform filtration device 10 is made up of (its schematic diagram is with accompanying drawing 1) uniform filtration bag 7 and Patting type homogenizer 13; As shown in fig. 6-7, its two-layer sampling bag 1 is inside and outside two layer 2,3, and bag top is provided with a tearing notch 4; Its internal layer 2 is set to reverse taper, and the bottom of this internal layer 2 upwards folds, and bottom 9 is fixed on the side outer wall of internal layer 2; The bottom of its internal layer 2 is also provided with a tearing notch 4; There is a filter membrane interlayer 8 inside of its uniform filtration bag 7; The middle part of this filter membrane interlayer 8 is fixed on the side bag of uniform filtration bag 7 along bag mouth to direction at the bottom of bag, has namely been separated out two filtration zones distributed side by side; The two ends of this filter membrane interlayer 8 and low side are all fixed on the bag of uniform filtration bag 7, and the filter membrane hole pore size on filter membrane interlayer 8 is 0.4mm; In its stage filter 12, the aperture of filtering net is arranged from big to small between 0.2mm��0.04mm scope.
During embody rule, adopt the first direct sampling of sterilizing two-layer sampling bag 1 special described in this example, after sampling, the tearing notch 4 on two-layer sampling bag 1 is torn and abandon outer bag 3, internal layer 2 is put into filter membrane interlayer 8 side of aseptic uniform filtration bag 7, tearing the tearing notch 4 on internal layer 2 bottom, faecal samples directly enters uniform filtration bag 7 from the passage that tearing notch 4 is formed; Weigh, add diluent, then realize sample comminution uniform filtration by Patting type homogenizer 13; Washing centrifugation in whizzer 11 more afterwards, by stage filter 12 classified filtering, centrifuge washing separation and classified filtering hocket, the parasitic ovum that may exist in filtering intestinal microflora liquid, i.e. obtained product, afterwards again through aseptic subpackaged.
Claims (10)
1. one kind obtains the extraction system of enteric microorganism bacterium group from human excrement and urine, it is characterised in that: it includes sampler bag (14), uniform filtration device (10) and whizzer (11); Described uniform filtration device (10) comprises uniform filtration bag (7) and homogenizer (13); There is filter membrane interlayer (8) inside of described uniform filtration bag (7).
2. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 1, it is characterised in that: the filter membrane hole pore size on described filter membrane interlayer (8) is 0.1mm��0.45mm.
3. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 1 or 2, it is characterised in that: it also includes strainer (12); Described strainer (12) is stage filter, and wherein filtering net aperture from big to small within the scope of 0.2mm��0.04mm arrange.
4. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 1 or 2, it is characterised in that: the two ends of described filter membrane interlayer (8) and low side are all fixed on the bag of described uniform filtration bag (7).
5. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 3, it is characterised in that: the two ends of described filter membrane interlayer (8) and low side are all fixed on the bag of described uniform filtration bag (7).
6. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 1 or 2, it is characterised in that: described homogenizer (13) is Patting type homogenizer.
7. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 1 or 2, it is characterised in that: described homogenizer (13) is Patting type homogenizer; Described sampler bag (14) is two-layer sampling bag (1); Described two-layer sampling bag (1) is inside and outside two layers (2,3), and its bag top is provided with tearing notch (4); The bottom of described internal layer (2) upwards folds, and bottom (9) are fixed on the side outer wall of internal layer (2); The bottom of described internal layer (2) is provided with a tearing notch (4); The bag top of described two-layer sampling bag (1) is provided with sealing strip (5) and tear opening (6); Described tear opening (6) is positioned at the top of described sealing strip (5).
8. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 3, it is characterised in that: described homogenizer (13) is Patting type homogenizer; Described sampler bag (14) is two-layer sampling bag (1); Described two-layer sampling bag (1) is inside and outside two layers (2,3), and its bag top is provided with tearing notch (4); The bottom of described internal layer (2) upwards folds, and bottom (9) are fixed on the side outer wall of internal layer (2); The bottom of described internal layer (2) is provided with a tearing notch (4); The bag top of described two-layer sampling bag (1) is provided with sealing strip (5) and tear opening (6); Described tear opening (6) is positioned at the top of described sealing strip (5).
9. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 4, it is characterised in that: described homogenizer (13) is Patting type homogenizer; Described sampler bag (14) is two-layer sampling bag (1); Described two-layer sampling bag (1) is inside and outside two layers (2,3), and its bag top is provided with tearing notch (4); The bottom of described internal layer (2) upwards folds, and bottom (9) are fixed on the side outer wall of internal layer (2); The bottom of described internal layer (2) is provided with a tearing notch (4); The bag top of described two-layer sampling bag (1) is provided with sealing strip (5) and tear opening (6); Described tear opening (6) is positioned at the top of described sealing strip (5).
10. from human excrement and urine, obtain the extraction system of enteric microorganism bacterium group as claimed in claim 5, it is characterised in that: described homogenizer (13) is Patting type homogenizer; Described sampler bag (14) is two-layer sampling bag (1); Described two-layer sampling bag (1) is inside and outside two layers (2,3), and its bag top is provided with tearing notch (4); The bottom of described internal layer (2) upwards folds, and bottom (9) are fixed on the side outer wall of internal layer (2); The bottom of described internal layer (2) is provided with a tearing notch (4); The bag top of described two-layer sampling bag (1) is provided with sealing strip (5) and tear opening (6); Described tear opening (6) is positioned at the top of described sealing strip (5).
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| Application Number | Priority Date | Filing Date | Title |
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| CN201520959785.5U CN205275584U (en) | 2015-11-27 | 2015-11-27 | Obtain intestinal microbial community's extraction system in human excrement and urine of follow |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399068A (en) * | 2016-08-31 | 2017-02-15 | 孟素兰 | Device for manual preparation of manure liquid |
| CN106811425A (en) * | 2015-11-27 | 2017-06-09 | 重庆北芝堂生物科技有限公司 | A kind of method that gut flora is separated from human excrement and urine |
| CN106190793B (en) * | 2016-08-31 | 2018-12-25 | 孟素兰 | A kind of liquid manure Preparation equipment |
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2015
- 2015-11-27 CN CN201520959785.5U patent/CN205275584U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106811425A (en) * | 2015-11-27 | 2017-06-09 | 重庆北芝堂生物科技有限公司 | A kind of method that gut flora is separated from human excrement and urine |
| CN106811425B (en) * | 2015-11-27 | 2020-10-09 | 重庆北芝堂生物科技有限公司 | Method for separating intestinal flora from human excrement |
| CN106399068A (en) * | 2016-08-31 | 2017-02-15 | 孟素兰 | Device for manual preparation of manure liquid |
| CN106399068B (en) * | 2016-08-31 | 2018-12-25 | 孟素兰 | A kind of device preparing liquid manure manually |
| CN106190793B (en) * | 2016-08-31 | 2018-12-25 | 孟素兰 | A kind of liquid manure Preparation equipment |
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