CN106479869A - a cell filter - Google Patents

Abstract

The invention relates to a cell filter, which comprises an upper collector and a filtering area, wherein the upper collector is cylindrical in shape, the filtering area is integrally formed with the lower end of the upper collector, the upper end of the upper collector is provided with a cover, a valve is arranged at the communication part of the upper collector and the filtering area, and the bottom of the filtering area is provided with a filter membrane; still include down the collector, down the collector with filter zone lower extreme can dismantle the connection. The structure of the cell filter enables the blowing, the filtering and the collecting to be completed at one time, the operation is simple and convenient, the possibility of pollution is reduced, the sterile requirement is better met, the simplicity and the strict sterile design of the operation are realized, and more convenient experimental conditions can be provided for experimenters.

Description

a cell filter

technical field

The present invention relates to a medical and laboratory appliance, in particular to a cell filter.

Background technique

Primary cell culture is one of the most commonly used methods in biological and medical research. Primary cell culture is the stage of culture that directly obtains cells, tissues and organs from organisms until they are cultured. Since the cells have just been separated from the living tissue, they are closer to the living conditions in the living body. This method can provide a powerful means for studying the growth, metabolism and reproduction of living cells, and also create conditions for subsequent subculture. Using this method It can also directly serve clinical practice. After separating and digesting tissues in animals, in addition to primary cells, there are generally not completely dispersed primitive tissues, organs, cell masses and culture fluids, so in the collection of products, it is necessary to first filter the mixed solution after pipetting. Filtration of the mixed solution requires the use of a cell filter, which can complete magazine filtration, cell dispersion, and sample separation.

Cell strainers provide a simple and easy method for single-cell suspensions in paper cups. The existing cell filter is filtered through a filter screen with a specific pore size, in which it is necessary to put a disposable cell filter screen/metal cell filter screen on the centrifuge tube, and use a straw to absorb and blow the cell suspension solution, drop it on a filter screen to filter, then absorb and collect the filtered cell suspension, and inoculate it into a petri dish.

The above steps are relatively separated, and the operation is complicated, especially for laboratory personnel with high requirements for aseptic operation; moreover, the metal cell filter screen used in the filtration process is often used repeatedly after autoclaving, and it is easy to produce bacteria after long-term use. Rust, causing pollution; for cell filter screens made of metal, in the prior art, the remaining cells and tissues on the filter screen are also cleaned by washing with dilute alkaline water, then soaked and cleaned with detergent solution, and finally rinsed with tap water Filtering, rinsing with distilled water and double distilled water, sterilizing the filtering and packaging, and then putting the filtering screen into the packaging for storage, this cleaning method is cumbersome and wastes manpower.

Contents of the invention

In view of this, the purpose of the present invention is to overcome the deficiencies of the prior art and provide an integrated cell filter, which can complete blowing, filtering, and collection at one time, is easy to operate, reduces the possibility of contamination, and better meets the requirements of sterility .

In order to achieve the above object, the present invention adopts the following technical scheme: a cell filter, comprising an upper collector shaped as a cylinder and a filter area integrally formed with the lower end of the upper collector, the upper end of the upper collector is provided with In the cover, a valve is provided at the connection between the upper collector and the filter area, and a filter membrane is provided at the bottom of the filter area; a lower collector is also included, and the lower collector is detachably connected to the lower end of the filter area.

Preferably, the inner thread on the inner side of the cover is threadedly connected to the outer thread on the outer surface of one end of the upper collector.

Preferably, the connection between the filter area and the lower collector is threaded connection, plug seal or interference fit, that is, the external thread on the outer surface of the lower end of the filter area is connected to the inner thread on the inner surface of the upper end of the lower collector. , or the lower end of the filter area is sealed and plugged into the upper end of the lower collector, or an interference fit, using the characteristics of thermal expansion and contraction to cover the upper end of the lower collector to the lower end of the filter area.

Preferably, the shape of the lower collector is a cylinder.

Preferably, the diameters of the upper collector and the lower collector are equal to the diameter of a 50ml centrifuge tube.

Preferably, the length of the upper collector is less than the length of the 50ml centrifuge tube.

Preferably, the filter membrane adopts a nylon mesh structure, and the cell filter is made of plastic material except for the filter membrane.

Preferably, the valve is a medical one-way valve.

Preferably, the shape of the filtering area is a cylinder or a cone.

The present invention adopts the above technical scheme, the upper collector is more convenient than the petri dish during the blowing process of the cells, and is not easy to overflow, and compared with the 50ml centrifuge tube, it removes the redundant length and saves materials; the sealing of the middle valve ensures that the device The inside is aseptic and separated from top to bottom; the diameter of the upper collector and the lower collector is the same as that of a 50ml centrifuge tube, and the cell filter can be directly inserted into the test tube rack for easy operation; the detachable connection between the filter area and the lower collector, It not only ensures the sterility inside the cell filter, but also can conveniently take out the lower collector to facilitate the collection of the filtered liquid. The structure of the cell filter of the present invention can complete blowing, filtering, and collection at one time, and is easy to operate, reduces the possibility of contamination, and is more in line with the requirements of sterility. The simplicity of operation and strict aseptic design can provide more Convenient experimental conditions.

Description of drawings

Fig. 1 is the schematic diagram of cell filter of the present invention;

Fig. 2 is a sectional view of the cell filter of the present invention.

In the figure: 1. Cover; 2. Upper collector; 3. Valve; 4. Filter membrane; 5. Lower collector; 6. Filtration area.

detailed description

In order to make the purpose, technical solution and advantages of the present invention clearer, the technical solution of the present invention will be described in detail below. Apparently, the described embodiments are only some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other implementations obtained by persons of ordinary skill in the art without making creative efforts fall within the protection scope of the present invention.

Embodiment 1, as shown in Figure 1 and Figure 2, a cell filter includes an upper collector 2 in the shape of a cylinder and a filter area 6 integrally formed with the lower end of the upper collector 2, the upper collector The upper end of the device 2 is provided with a cover 1, a valve 3 is provided at the connection between the upper collector 2 and the filter area 6, and a filter membrane 4 is provided at the bottom of the filter area 6; a lower collector 5 is also included, and the lower collector 5 It is detachably connected with the lower end of the filter area 6 .

Embodiment 2, a cell filter, including an upper collector 2 in the shape of a cylinder and a filter area 6 integrally formed with the lower end of the upper collector 2, the upper end of the upper collector 2 is provided with a cover 1, the A valve 3 is provided at the connection between the collector 2 and the filter area 6, and a filter membrane 4 is provided at the bottom of the filter area 6;

It also includes a lower collector 5, which is detachably connected to the lower end of the filter area 6. The inner thread on the inside of the cover 1 is threadedly connected to the outer thread on the outer surface of one end of the upper collector 2 . The connection between the filter area 6 and the lower collector 5 is threaded connection, plug seal or interference fit, that is, the external thread on the outer surface of the lower end of the filter area 6 and the inner thread on the inner surface of the upper end of the lower collector 5 Connection, or the lower end of the filter area 6 is sealed and plugged into the upper end of the lower collector 5, or an interference fit, the upper end of the lower collector 5 is sleeved on the filter area 6 by utilizing the characteristics of thermal expansion and contraction the lower end.

In this embodiment, the shape of the lower collector 5 is a cylinder.

It should be noted that the diameters of the upper collector 2 and the lower collector 5 are equal to the diameter of the 50ml centrifuge tube, and the length of the upper collector 2 is less than the length of the 50ml centrifuge tube.

It should be added that the filter membrane 4 adopts a nylon mesh structure, and the cell filter except the filter membrane 4 is made of plastic.

It can be understood that the valve 3 is a medical one-way valve.

In addition, the shape of the filtering area 6 is a cylinder or a cone.

In actual use, first unscrew the cap 1, flush the bone marrow fluid into the upper collector 2 with a syringe, blow the bone marrow cells evenly in the upper collector 2 with a disposable dropper, then open the valve 3, and the cell suspension enters the filter Zone 6, after being filtered by the filter membrane 4, it flows into the lower collector 5, unscrew the lower collector 5, absorb the filtered single cell suspension, and plant it into a culture bottle/petri dish. It can be seen that the use of the cell filter of the present invention can complete pipetting, filtration, and collection at one time, which is easy to operate, reduces the possibility of contamination, and better meets the requirements of sterility.

The present invention adopts the above technical scheme, the upper collector is more convenient than the petri dish during the blowing process of the cells, and is not easy to overflow, and compared with the 50ml centrifuge tube, it removes the redundant length and saves materials; the sealing of the middle valve ensures that the device The internal sterility and upper and lower separation; the detachable connection between the filter area and the lower collector not only ensures the sterility inside the cell filter, but also allows the lower collector to be easily removed to collect the filtered liquid. The structure of the cell filter of the present invention can complete blowing, filtering, and collection at one time, and is easy to operate, reduces the possibility of contamination, and is more in line with the requirements of sterility. The simplicity of operation and strict aseptic design can provide more Convenient experimental conditions.

The specific embodiments described above have further described the purpose, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above descriptions are only specific embodiments of the present invention and are not intended to limit the scope of the present invention. Protection scope, within the spirit and principles of the present invention, any modification, equivalent replacement, improvement, etc., shall be included in the protection scope of the present invention.

Claims (10)

1. a kind of cell filter it is characterised in that include a shape be in cylindrical upper catcher and with described upper collection The integrally formed filtering area in device lower end, described upper catcher upper end is provided with lid, and described upper catcher and filtering area connectivity part set There is a valve, described filtering area bottom is provided with filter membrane；Also include lower catcher, described lower catcher and described filtering area lower end can Dismounting connects.

2. cell filter according to claim 1 is it is characterised in that female thread inside described lid and described upper receipts The external screw thread of storage one end outer surface is threaded connection.

3. cell filter according to claim 1 it is characterised in that described lower catcher and filtering area lower end removable Mode of unloading is threaded, plug envelope or interference fit.

4. cell filter according to claim 1 is it is characterised in that described lower catcher is shaped as cylinder.

5. cell filter according to claim 4 is it is characterised in that diameter of described upper catcher and lower catcher etc. Diameter in 50ml centrifuge tube.

6. cell filter according to claim 1 is it is characterised in that the length of described upper catcher is less than 50ml centrifugation The length of pipe.

7. the cell filter according to any one of claim 1 to 6 is it is characterised in that described filter membrane adopts nylon screen Structure.

8. the cell filter according to any one of claim 1 to 6 is it is characterised in that described cell filter removes filter membrane Outside all using plastic material.

9. the cell filter according to any one of claim 1 to 6 is it is characterised in that described valve is Medical check valve.

10. the cell filter according to any one of claim 1 to 6 is it is characterised in that described filtering area is shaped as cylinder Body or vertebral body.