CN209518052U - A kind of tiny seed disinfection case efficiently, easy - Google Patents
A kind of tiny seed disinfection case efficiently, easy Download PDFInfo
- Publication number
- CN209518052U CN209518052U CN201821836982.8U CN201821836982U CN209518052U CN 209518052 U CN209518052 U CN 209518052U CN 201821836982 U CN201821836982 U CN 201821836982U CN 209518052 U CN209518052 U CN 209518052U
- Authority
- CN
- China
- Prior art keywords
- seeds
- tube
- inner tube
- cylindrical inner
- easy
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 27
- 239000012528 membrane Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 7
- 238000004161 plant tissue culture Methods 0.000 abstract description 6
- 239000002699 waste material Substances 0.000 abstract description 3
- 241000201976 Polycarpon Species 0.000 abstract description 2
- 239000007788 liquid Substances 0.000 description 8
- 241000196324 Embryophyta Species 0.000 description 7
- 230000000249 desinfective effect Effects 0.000 description 4
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 description 4
- 239000008223 sterile water Substances 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 229910001961 silver nitrate Inorganic materials 0.000 description 2
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 2
- 241000219195 Arabidopsis thaliana Species 0.000 description 1
- 241000208125 Nicotiana Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000011012 sanitization Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本实用新型是一种高效、简便的细小种子消毒容器装置，其特征在于：由顶部带盖、底部为滤膜的圆柱形内管与圆柱形外管组合而成，所述的圆柱形内管由顶部的盖子、管体以及管体底部的滤膜构成。本实用新型所述消毒容器装置能够解决普通离心管装置消毒细小种子容易造成种子的浪费及操作相对比较繁琐问题，以及处理较多种子时费时费力的问题。因此，本实用新型具有植物组培过程中对细小种子进行消毒时具有高效、操作简便等优点。
The utility model is an efficient and convenient small seed disinfection container device, which is characterized in that it is composed of a cylindrical inner tube with a cover on the top and a filter membrane at the bottom and a cylindrical outer tube. The cylindrical inner tube It consists of a cap at the top, a tube body, and a filter membrane at the bottom of the tube body. The disinfection container device of the utility model can solve the problem that the disinfection of small seeds by the common centrifuge tube device is easy to cause the waste of seeds and the operation is relatively cumbersome, and the problem of time-consuming and labor-intensive processing of many seeds. Therefore, the utility model has the advantages of high efficiency, easy operation and the like when the tiny seeds are sterilized during the plant tissue culture process.
Description
技术领域technical field
本实用新型涉及一种高效、简便的细小种子消毒容器装置，属于植物组织培养领域。The utility model relates to an efficient and convenient small seed disinfection container device, which belongs to the field of plant tissue culture.
背景技术Background technique
在植物组织培养过程中，通常是利用植物无菌苗作为外植体，因此为获得植物无菌苗，需要我们对植物种子进行消毒后，再进行无菌培养。目前对于植物种子消毒通常是将75%酒精与一定浓度的次氯酸钠或硝酸银等试剂结合使用。对于细小种子的消毒通常使用1.5-2.0mL离心管作为容器装置，但普通离心管在消毒细小种子时用移液枪吸取液体时，种子容易被吸出而损失。并且在消毒种子时，通常用消毒液及无菌水清洗，需要反复吸取液体7-9次，用普通离心管装置消毒细小种子容易造成种子的损失及操作相对比较繁琐。为解决此类问题，我们设计了一种高效、简便的细小种子消毒容器装置。In the process of plant tissue culture, plant sterile seedlings are usually used as explants, so in order to obtain plant sterile seedlings, we need to sterilize the plant seeds before aseptic culture. At present, for the disinfection of plant seeds, 75% alcohol is usually used in combination with reagents such as sodium hypochlorite or silver nitrate of a certain concentration. For the disinfection of fine seeds, a 1.5-2.0mL centrifuge tube is usually used as a container device, but when ordinary centrifuge tubes are used to suck up liquid with a pipette gun when disinfecting fine seeds, the seeds are easily sucked out and lost. And when disinfecting seeds, they are usually cleaned with disinfectant and sterile water, and the liquid needs to be repeatedly absorbed 7-9 times. Disinfecting small seeds with an ordinary centrifuge tube device is likely to cause loss of seeds and the operation is relatively cumbersome. In order to solve this type of problem, we have designed an efficient and simple small seed disinfection container device.
发明内容Contents of the invention
本实用新型的目的在于提供一种高效、简便的细小种子消毒容器装置；该消毒容器装置将解决普通离心管装置消毒细小种子容易造成种子的浪费及操作相对比较繁琐问题，以及处理较多种子时费时费力的问题，从而达到高效、操作简便等目的。The purpose of this utility model is to provide a kind of efficient, simple and convenient small seed disinfection container device; This disinfection container device will solve the problem that common centrifuge tube device disinfection of small seeds is easy to cause the waste of seeds and relatively cumbersome operation, and when processing more seeds Time-consuming and labor-intensive problems, so as to achieve high efficiency and easy operation.
为解决上述问题，本实用新型的技术方案为：一种高效、简便的细小种子消毒容器装置，由顶部带盖、底部为滤膜的圆柱形内管与圆柱形外管组合而成，所述的圆柱形内管由顶部的盖子、管体以及管体底部的滤膜构成。In order to solve the above-mentioned problems, the technical solution of the utility model is: a kind of efficient and convenient small seed disinfection container device, which is composed of a cylindrical inner tube with a cover on the top and a filter membrane at the bottom and a cylindrical outer tube. The cylindrical inner tube consists of a cap at the top, a tube body, and a filter membrane at the bottom of the tube body.
所述的滤膜可以选用水系膜等滤膜。The filter membrane can be selected from aqueous membrane and other filter membranes.
所述的圆柱形内管管体高度为1.5-2.0cm。The height of the cylindrical inner tube body is 1.5-2.0cm.
所述的圆柱形外管的内径大于圆柱形内管的外径，圆柱形外管的高度大于圆柱形内管的高度，圆柱形外管高度为2.0-2.5cm。The inner diameter of the cylindrical outer tube is greater than the outer diameter of the cylindrical inner tube, the height of the cylindrical outer tube is greater than the height of the cylindrical inner tube, and the height of the cylindrical outer tube is 2.0-2.5 cm.
本实用新型所述消毒容器装置能够解决普通离心管装置消毒细小种子容易造成种子的浪费及操作相对比较繁琐问题，以及处理较多种子时费时费力的问题。因此，本实用新型具有植物组培过程中对细小种子进行消毒时具有高效、操作简便等优点。The disinfection container device of the utility model can solve the problem that the disinfection of small seeds by the common centrifuge tube device is easy to cause the waste of seeds and the operation is relatively cumbersome, and the problem of time-consuming and labor-intensive processing of many seeds. Therefore, the utility model has the advantages of high efficiency, easy operation and the like when the tiny seeds are sterilized during the plant tissue culture process.
附图说明Description of drawings
为了更清楚地说明本实用新型实施例或现有技术中的技术方案，下面将对实施例或现有技术描述中所需要使用的附图作简单地介绍，显而易见地，对于本领域普通技术人员来讲，在不付出创造性劳动的前提下，还可以根据这些附图获得其他的附图。In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings that need to be used in the description of the embodiments or the prior art. Obviously, for those of ordinary skill in the art Generally speaking, other drawings can also be obtained based on these drawings on the premise of not paying creative work.
图1是本实用新型的结构示意图。Fig. 1 is the structural representation of the utility model.
图2是本实用新型的圆柱形内管结构示意图。Fig. 2 is a structural schematic diagram of a cylindrical inner tube of the present invention.
图3是本实用新型的圆柱形外管结构示意图。Fig. 3 is a structural schematic diagram of a cylindrical outer tube of the present invention.
图中：1-圆柱形内管，2-圆柱形外管，1a -滤膜，1b-圆柱形内管管体，1c-顶部管盖。In the figure: 1-cylindrical inner tube, 2-cylindrical outer tube, 1a-filter membrane, 1b-cylindrical inner tube body, 1c-top cap.
具体实施方式Detailed ways
下面结合实施例对本实用新型作进一步的详细描述。Below in conjunction with embodiment the utility model is described in further detail.
如图1所示，本实用新型所述的细小种子消毒容器装置，由圆柱形内管1和圆柱形外管2组合而成，圆柱形外管2高度为2.0-2.5cm，圆柱形内管1外径小于圆柱形外管2内径2-3mm，圆柱形内管底部的滤膜1a设置为水系膜等滤膜；圆柱形内管管体1b高度为1.5-2.0cm；圆柱形内管顶部管盖1c能将内管盖好。As shown in Figure 1, the tiny seed disinfection container device described in the utility model is composed of a cylindrical inner tube 1 and a cylindrical outer tube 2. The height of the cylindrical outer tube 2 is 2.0-2.5cm, and the cylindrical inner tube 1 The outer diameter is smaller than the cylindrical outer tube 2 The inner diameter is 2-3mm, and the filter membrane 1a at the bottom of the cylindrical inner tube is set as a filter membrane such as an aqueous membrane; the height of the cylindrical inner tube body 1b is 1.5-2.0cm; the top of the cylindrical inner tube The tube cover 1c can cover the inner tube.
植物组织培养常见的细小植物种子如：拟南芥种子，呈卵形，长约1毫米(mm)；烟草种子，呈圆形或宽矩圆形，径约0.5毫米(mm)。本实用新型在使用时，在植物组织培养试验中用于细小种子消毒阶段，通常在超净工作台中进行种子消毒过程，通常将种子倒入普通离心管，然后进行1次添加75%酒精进行消毒30s，后吸出，用无菌水清洗1-2次，再加入一定浓度的次氯酸钠或硝酸银消毒10-15min，吸出后，再进行无菌水清洗4-5次，普通离心管容器装置在液体反复吸取7-9次时，会有种子在此过程中被吸出而损失。而本实用新型不会出现此类情况，因为在内管管中倒入需要消毒的植物种子，内管的作用就是将加入的种子被加入消毒液体浸泡，待消毒时间达到，外管与内管配套，在离心机中进行离心，将消毒液体与种子进行分离，而滤膜主要是可以将液体滤出，而种子仍在内管内，外管的作用主要是收集液体，因此消毒装置可以达到种子与消毒液体等的较好分离而不损失种子的效果。Small plant seeds common in plant tissue culture are: Arabidopsis thaliana seeds, oval in shape, about 1 millimeter (mm) in length; tobacco seeds, in round or wide oblong shape, about 0.5 mm in diameter. When the utility model is in use, it is used in the disinfection stage of tiny seeds in the plant tissue culture test. Usually, the seed disinfection process is carried out in the ultra-clean workbench. Usually, the seeds are poured into a common centrifuge tube, and then 75% alcohol is added once for disinfection. After 30s, suck it out, wash it with sterile water for 1-2 times, then add a certain concentration of sodium hypochlorite or silver nitrate for disinfection for 10-15 minutes, after sucking it out, wash it with sterile water for 4-5 times. When sucking repeatedly 7-9 times, some seeds will be sucked out and lost in the process. And this kind of situation can not occur in the present utility model, because the plant seed that needs disinfection is poured into in the inner pipe pipe, the effect of inner pipe is exactly that the seed that adds is added to be soaked in sterilizing liquid, until the disinfection time reaches, outer pipe and inner pipe Matching, centrifuge in a centrifuge to separate the disinfection liquid from the seeds, and the filter membrane can mainly filter out the liquid, while the seeds are still in the inner tube, and the function of the outer tube is mainly to collect the liquid, so the disinfection device can reach the seed Better separation from sanitizing liquids etc. without loss of seed effect.
本实用新型适用于对大量细小植物种子消毒过程，不仅高效、操作方便而且种子也不会因反复添加或吸出液体而损失，因此本实用新型是一种高效、简便的细小种子消毒容器装置。The utility model is suitable for the process of disinfecting a large number of small plant seeds. It is not only efficient and easy to operate, but also the seeds will not be lost due to repeated addition or suction of liquid. Therefore, the utility model is an efficient and convenient small seed disinfection container device.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821836982.8U CN209518052U (en) | 2018-11-08 | 2018-11-08 | A kind of tiny seed disinfection case efficiently, easy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201821836982.8U CN209518052U (en) | 2018-11-08 | 2018-11-08 | A kind of tiny seed disinfection case efficiently, easy |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209518052U true CN209518052U (en) | 2019-10-22 |
Family
ID=68211689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201821836982.8U Active CN209518052U (en) | 2018-11-08 | 2018-11-08 | A kind of tiny seed disinfection case efficiently, easy |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209518052U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145097A (en) * | 2022-01-11 | 2022-03-08 | 河南省农业科学院烟草研究所 | Device and method for efficiently screening high-quality tobacco seeds |
-
2018
- 2018-11-08 CN CN201821836982.8U patent/CN209518052U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114145097A (en) * | 2022-01-11 | 2022-03-08 | 河南省农业科学院烟草研究所 | Device and method for efficiently screening high-quality tobacco seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN209518052U (en) | A kind of tiny seed disinfection case efficiently, easy | |
| CN106479869A (en) | a cell filter | |
| CN202285519U (en) | Plant small seed sterilization device | |
| CN102018969B (en) | Device for disinfecting surfaces of explants of plants | |
| CN103868751A (en) | Clostridium difficile infects sample sampling preliminary treatment mixing arrangement | |
| CN206262194U (en) | A kind of phytopathogen separates the device for blade surface sterilization | |
| CN104904604A (en) | Disinfection and dewatering device for tissue culture of explants | |
| CN210876650U (en) | A baby bottle and nipple cleaning device | |
| CN207321824U (en) | A kind of convenient sterilizer of Orchid Seeds | |
| CN204762668U (en) | Group banks up explant disinfection and dewatering device with earth | |
| CN205163887U (en) | Automatic control oral cavity sterilizing equipment | |
| CN203212561U (en) | Waste liquid culture medium sucking device for laboratory | |
| CN205922358U (en) | Explant sterilizing device | |
| CN218529359U (en) | A kit for surface disinfection of bulk plant pathogenic bacteria isolation samples | |
| CN102166367A (en) | Method for AMF spore surface disinfection | |
| CN206414531U (en) | It is a kind of to be used for the chlorination equipment of plant haulm and the surface of the seed | |
| CN222348975U (en) | Plant callus device is infected to agrobacterium | |
| CN109169290A (en) | A kind of method and chlorination equipment of efficient, easy tiny seed disinfection | |
| CN219614502U (en) | Disposable medical drainage liquid sterilizing barrel | |
| CN204379485U (en) | A kind of zoopery surgical wound hair cleaning means | |
| CN214441622U (en) | Cleaning device of liquid food sampler | |
| CN218501035U (en) | Insect sample disinfection cleaning device | |
| CN207666894U (en) | A kind of glass pipette sterilization cylinder | |
| CN223166386U (en) | Sampling device for milk sterilization post-inspection | |
| CN221787538U (en) | A device for collecting and cleaning waste liquid from internal medicine drainage |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |