CN112940914A

CN112940914A - Experimental device for promote differentiation of epidermal stem cells and growth

Info

Publication number: CN112940914A
Application number: CN202110125587.9A
Authority: CN
Inventors: 赵彬
Original assignee: Individual
Current assignee: Individual
Priority date: 2021-01-29
Filing date: 2021-01-29
Publication date: 2021-06-11

Abstract

The invention discloses an experimental device for promoting differentiation and growth of epidermal stem cells, and relates to the technical field of cell culture. This promote experimental apparatus of epidermis stem cell differentiation and growth, first loading board accessible first motor comes the rotation regulation position, and the vacuum chuck of cooperation liftable adsorbs to putting thing groove upper surface filter box and snatchs, thereby the culture dish of convenience and below carries out the block and is connected, and first loading board upper surface encircles the hole site that distributes and have a plurality of crisscross distributions and puts the thing groove, be favorable to improving the separation efficiency of device to cell tissue, and put the thing groove and still can deposit the abandonment culture dish of follow-up separation, the finishing performance of device has been improved.

Description

Experimental device for promote differentiation of epidermal stem cells and growth

Technical Field

The invention relates to the technical field of cell culture, in particular to an experimental device for promoting differentiation and growth of epidermal stem cells.

Background

The cell culture is a method for simulating in-vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to ensure that the in-vivo environment can survive, grow, reproduce and maintain the main structure and functions, cell tissues need to be collected in the process of culturing the epidermal stem cells to perform operation and culture in the sterile environment, the process has higher requirements on the operation skills and the sanitary conditions of workers, and meanwhile, the damage of external bacteria to the cell culture environment is avoided.

At present, corresponding operation is usually carried out manually in the stem cell differentiation culture process, the requirement on the professional skill of workers in the process is high, and the culture environment can be polluted in the process of each operation step, so that the culture effect and efficiency are low, and aiming at the conditions, an experimental device for promoting the differentiation and growth of epidermal stem cells is provided.

Disclosure of Invention

Aiming at the defects of the prior art, the invention provides an experimental device for promoting differentiation and growth of epidermal stem cells, and solves the problems that the prior stem cell differentiation culture process is generally operated manually, the requirement on the professional skills of workers is high, and the culture environment can be polluted in each operation step process, so that the culture effect and efficiency are low.

In order to achieve the purpose, the invention is realized by the following technical scheme: an experimental device for promoting differentiation and growth of epidermal stem cells comprises a base and a second support rod, wherein a first support rod is fixed on the upper surface of the base, a first motor is arranged at the upper end of the first support rod, a first bearing plate is connected to the upper end of the first motor, hole sites are formed in the edge of the upper surface of the first bearing plate, object placing grooves are formed in the intervals of the hole sites, filter boxes are arranged on the upper surfaces of the object placing grooves, a bearing rod is connected to the middle of the upper surface of the first bearing plate, a first electric telescopic rod is arranged at the tail end of the bearing rod, a second motor is connected to the lower end of the first electric telescopic rod, a vacuum sucker is connected to the lower end of the second motor, a connector is connected to the opening end of each filter box, a thread groove is formed in the inner wall of the connector, a filter screen is fixed below the, the culture dish is attached to the inner wall of the clamping groove, a first air cylinder is arranged in the first supporting rod, the tail end of the first air cylinder is connected with a second bearing plate, a third motor is arranged in the tail end of the second bearing plate, the upper end of the third motor is connected with a second air cylinder, the upper end of the second air cylinder is connected with a fourth motor, the tail end of the fourth motor is connected with a pneumatic chuck, the second supporting rod is arranged at the right side of the first supporting rod, a fifth motor is arranged in the second supporting rod, the upper end of the fifth motor is connected with a connecting shaft, a centrifugal test tube seat is fixed on the outer wall of one side of the connecting shaft, a culture bottle seat is fixed on the outer wall of the other side of the connecting shaft, a sixth motor is arranged in the tail end of the second supporting rod, a moving rod is connected with the upper end of the, the utility model discloses a reagent box, including first chuck, second electric telescopic handle, base upper surface, rubber head straw, second support bar, second electric telescopic handle lower extreme is connected with two-way cylinder, and two-way cylinder end-to-end connection has first chuck, the inner chamber has been seted up to first chuck inside, and inner chamber inner wall is provided with the gasbag, the gasbag upper end is connected with the air pump, the arrangement of base upper surface has rubber head straw, and the right-hand waste liquid case that is provided with of rubber head.

Optionally, the first bearing plate forms a rotating structure with the first support rod through the first motor, and hole sites are distributed on the upper surface of the first bearing plate in a surrounding manner, and are distributed in a staggered manner with the storage slots.

Optionally, the upper surface of the storage groove is attached to the lower surface of the filter box, and the size of the outer opening of the filter box is smaller than that of the inner opening of the hole site.

Optionally, the vacuum chuck forms a lifting structure through between first electric telescopic handle and the carrier bar, and the vacuum chuck forms a rotating structure through between second motor and the first electric telescopic handle, is parallel distribution moreover between vacuum chuck and the filter box.

Optionally, the filter cartridge passes through to constitute the active structure between thread groove and the connector, and the filter cartridge passes through to constitute the block structure between draw-in groove and the culture dish, and the internal orifice size of draw-in groove is identical with the external orifice size of filter screen moreover.

Optionally, the second bearing plate and the first support rod form a movable structure through the first cylinder, and the second bearing plate and the pneumatic chuck are movably connected.

Optionally, the movable rod forms an adjustable structure through the sixth motor and the second support rod, the vertical center line of the movable rod is overlapped with the vertical center line of the connecting shaft, and the connecting shaft forms a rotating structure through the fifth motor.

Optionally, the first chuck forms a sliding structure with the moving rod through the sliding block, the first chuck forms a moving structure with the sliding block through the bidirectional cylinder, and the first chuck is symmetrically distributed about a vertical center line of the bidirectional cylinder.

Optionally, the size of the inner opening of the inner cavity is matched with the size of the outer opening of the upper end of the rubber head suction pipe, and the outer surface of the rubber head suction pipe is attached to the outer surface of the air bag.

The invention provides an experimental device for promoting differentiation and growth of epidermal stem cells, which has the following beneficial effects:

1. this promote experimental apparatus of epidermis stem cell differentiation and growth, first loading board accessible first motor comes the rotation regulation position, and the vacuum chuck of cooperation liftable adsorbs to putting thing groove upper surface filter box and snatchs, thereby the culture dish of convenience and below carries out the block and is connected, and first loading board upper surface encircles the hole site that distributes and have a plurality of crisscross distributions and puts the thing groove, be favorable to improving the separation efficiency of device to cell tissue, and put the thing groove and still can deposit the abandonment culture dish of follow-up separation, the finishing performance of device has been improved.

2. This promote experimental apparatus of epidermal stem cell differentiation and growth, the rotatable pneumatic chuck of setting can rotate the filtration box and the culture dish of joint connection and invert, the inside filter screen that sets up of cooperation connector can carry out filtration treatment to the cell tissue in the culture dish, and can rotate the abandonment culture dish that finishes to filter through rotatable vacuum chuck and break away from, the pneumatic chuck of centre gripping can guarantee the filtration box stability of rotatory process simultaneously, and the culture dish that breaks away from can carry filter screen and filtration tissue together to transporting to putting the thing inslot and store, its cell filtration operation need not manual operation, and the filtration efficiency is improved.

3. This promote experimental apparatus of epidermis stem cell differentiation and growth, thereby the gasbag accessible air pump of the inside setting of first chuck lets in the gas of corresponding volume and makes the gasbag inflation and extrude and glue the first straw, makes it can carry out the reagent absorption of appointed volume, has effectively improved the interpolation accuracy of cell culture reagent, makes the cultivation effect more to the drive of cooperation two-way cylinder can carry out high-efficient clamp to gluing the first straw and get and the unloading.

4. This promote experimental apparatus of epidermal stem cell differentiation and growth can make the chuck can drive the rubber head straw and absorb different reagents through the portable slider that sets up and rotatable carriage release lever to the transfer operation of corresponding solution, and drive the centrifugal test tube seat through rotatable connecting axle and rotate and vibrate, thereby improve reagent dissolving effect, guarantee the experiment effect.

5. This promote experimental apparatus of epidermal stem cell differentiation and growth, a large amount of manual operation have been saved to the device, realize semi-automatic manual operation through automatic filtration device and the automatic device that adds the reagent, have reduced the risk that cell solution received the pollution among the manual operation, and come the control through the chuck of being equipped with the gasbag and glue the volume that the first straw absorbs the reagent, have improved the precision that the reagent added greatly, guarantee the effect of follow-up cell differentiation and growth.

Drawings

FIG. 1 is an overall schematic view of the present invention;

FIG. 2 is a schematic view of an upper surface structure of the first carrier plate according to the present invention;

FIG. 3 is a schematic view of the engagement structure of the filter cassette and the culture dish according to the present invention;

FIG. 4 is a schematic cross-sectional view of the connector of the present invention;

FIG. 5 is a schematic view showing the clamping state between the chuck and the plastic pipette of the present invention;

FIG. 6 is an enlarged view of the structure at A in FIG. 1 according to the present invention.

In the figure: 1. a base; 2. a first support bar; 3. a first motor; 4. a first bearing plate; 5. hole site; 6. a storage groove; 7. a filter cartridge; 8. a carrier bar; 9. a first electric telescopic rod; 10. a second motor; 11. a vacuum chuck; 12. a connector; 13. a thread groove; 14. a filter screen; 15. a card slot; 16. a culture dish; 17. a first cylinder; 18. a second carrier plate; 19. a third motor; 20. a second cylinder; 21. a fourth motor; 22. a pneumatic chuck; 23. a second support bar; 24. a fifth motor; 25. a connecting shaft; 26. centrifuging the test tube seat; 27. a culture bottle seat; 28. a sixth motor; 29. a travel bar; 30. a slider; 31. a second electric telescopic rod; 32. a bidirectional cylinder; 33. a first chuck; 34. an inner cavity; 35. an air bag; 36. an air pump; 37. a rubber head straw; 38. a waste liquid tank; 39. and (4) a reagent rack.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.

In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected" and "connected" are to be interpreted broadly, e.g., as being fixed or detachable or integrally connected; can be mechanically or electrically connected; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1 to 6, the present invention provides a technical solution: an experimental device for promoting differentiation and growth of epidermal stem cells comprises a base 1 and a second support rod 23, wherein a first support rod 2 is fixed on the upper surface of the base 1, a first motor 3 is arranged at the upper end of the first support rod 2, a first bearing plate 4 is connected with the upper end of the first motor 3, a hole site 5 is arranged at the edge of the upper surface of the first bearing plate 4, a storage groove 6 is arranged at the interval of the hole site 5, a filter box 7 is arranged on the upper surface of the storage groove 6, a bearing rod 8 is connected with the middle part of the upper surface of the first bearing plate 4, a first electric telescopic rod 9 is arranged at the tail end of the bearing rod 8, a second motor 10 is connected with the lower end of the first electric telescopic rod 9, a vacuum suction cup 11 is connected with the lower end of the second motor 10, a rotating structure is formed between the first bearing plate 4 and the first support rod 2 through the first motor 3, hole sites 5 are distributed on the upper surface of the first, the upper surface of the object placing groove 6 is attached to the lower surface of the filter box 7, the size of the outer opening of the filter box 7 is smaller than that of the inner opening of the hole site 5, the vacuum chuck 11 forms a lifting structure through the first electric telescopic rod 9 and the bearing rod 8, the vacuum chuck 11 forms a rotating structure through the second motor 10 and the first electric telescopic rod 9, the vacuum chuck 11 and the filter box 7 are distributed in parallel, in the process of culturing the dry cells, the collected tissue sample can be placed into the culture dish 16, 2ML 0.25% trypsin is added for digestion for 5-7min, 1ML of culture medium is added for terminating digestion, the pretreated culture dish 16 is placed on the upper surface of the second bearing plate 18 for facilitating subsequent filtration treatment, because the first electric telescopic rod 9 is arranged at the tail end of the bearing rod 8 on the upper surface of the first bearing plate 4, the vacuum chuck 11 at the lower end is favorable for driving the filter box 7 on the upper surface of the first bearing plate 4, the first bearing plate 4 can be rotated and adjusted by the driving of the first motor 3, so that the hole 5 formed in the upper surface of the first bearing plate is positioned below the filter box 7, and the filter box 7 adsorbed at the lower end of the first electric telescopic rod 9 can be conveniently pushed by the first electric telescopic rod 9 to descend to the culture dish 16 on the upper surface of the second bearing plate 18 through the hole 5 for preparing the filtering operation;

the open end of the filter box 7 is connected with a connector 12, the inner wall of the connector 12 is provided with a thread groove 13, a filter screen 14 is fixed below the thread groove 13, the inner wall of the connector 12 is provided with a clamping groove 15, the clamping groove 15 is positioned below the thread groove 13, the inner wall of the clamping groove 15 is attached with a culture dish 16, a first air cylinder 17 is arranged in the first supporting rod 2, the tail end of the first air cylinder 17 is connected with a second bearing plate 18, the tail end of the second bearing plate 18 is internally provided with a third motor 19, the upper end of the third motor 19 is connected with a second air cylinder 20, the upper end of the second air cylinder 20 is connected with a fourth motor 21, the tail end of the fourth motor 21 is connected with a pneumatic chuck 22, the filter box 7 forms a movable structure through the thread groove 13 and the connector 12, the filter box 7 forms a clamping structure through the clamping groove 15 and the culture dish 16, the second bearing plate 18 forms a movable structure with the first supporting rod 2 through the first cylinder 17, and the second bearing plate 18 is movably connected with the pneumatic chuck 22, the filter box 7 pushed by the first electric telescopic rod 9 can be connected with the upper end of the culture dish 16 through the connecting head 12 connected with the lower end of the filter box, meanwhile, the vacuum chuck 11 loses the adsorption force with the filter box 7 and is separated from the filter box 7 through the driving of the first electric telescopic rod 9, the pneumatic chuck 22 above the second bearing plate 18 can be rotated to the upper part of the filter box 7 through the driving of the third motor 19, and the pneumatic chuck 22 contacts the upper surface of the filter box 7 to carry out spacing clamping through the contraction of the second cylinder 20, because the upper end of the second cylinder 20 is connected with the fourth motor 21, the fourth motor 21 is convenient to drive the culture dish 16 on the upper surface of the second bearing plate 18 and the filter box 7 to rotate together for 180 degrees, thereby the cell tissue inside the culture dish 16 is filtered by the filter screen 14 in the connecting head 12, the filtered filtrate flows into the filter box 7 for storage, the filtered vacuum chuck 11 descends again and adsorbs the upper surface of the culture dish 16, the second motor 10 drives the vacuum chuck 11 and the culture dish 16 adsorbed by the vacuum chuck to rotate, the rotating culture dish 16 drives the connector 12 connected with the rotating culture dish 16 to unscrew from the upper end of the filter box 7 and separate, the filtered culture dish 16 is placed in the storage groove 6 on the upper surface of the first bearing plate 4 through the rising of the first electric telescopic rod 9, and filtered cell sap is obtained in the filter box 7 at the moment;

the second support bar 23 is arranged at the right side of the first support bar 2, a fifth motor 24 is arranged inside the second support bar 23, the upper end of the fifth motor 24 is connected with a connecting shaft 25, a centrifugal test tube seat 26 is fixed on the outer wall of one side of the connecting shaft 25, a culture bottle seat 27 is fixed on the outer wall of the other side of the connecting shaft 25, a sixth motor 28 is arranged inside the tail end of the second support bar 23, a moving rod 29 is connected with the upper end of the sixth motor 28, the outer wall of the moving rod 29 is connected with a slide block 30, a second electric telescopic rod 31 is fixed on the outer wall of the slide block 30, the lower end of the second electric telescopic rod 31 is connected with a bidirectional cylinder 32, the tail end of the bidirectional cylinder 32 is connected with a first chuck 33, an inner cavity 34 is arranged inside the first chuck 33, an air bag 35 is arranged on the inner wall of the inner cavity 34, a reagent rack 39 is arranged on the right of the second support rod 23, the movable rod 29 and the second support rod 23 form an adjustable structure through the sixth motor 28, the vertical center line of the movable rod 29 is coincident with the vertical center line of the connecting shaft 25, the connecting shaft 25 forms a rotating structure through the fifth motor 24, the first chuck 33 forms a sliding structure through the sliding block 30 and the movable rod 29, the first chuck 33 forms a movable structure through the bidirectional cylinder 32 and the sliding block 30, the first chucks 33 are symmetrically distributed about the vertical center line of the bidirectional cylinder 32, the size of the inner opening of the inner cavity 34 is coincident with the size of the outer opening of the upper end of the rubber head suction pipe 37, the outer surface of the rubber head suction pipe 37 is attached to the outer surface of the air bag 35, the filtered filter box 7 can be pushed by the first cylinder 17 to extend to the lower part of the first chuck 33 for transportation, and the first chuck 33 can move downwards through the drive of the second electric telescopic rod 31 and can drive the bidirectional cylinder 32 to clean the lower part The air bag 35 arranged in the first chuck 33 can be inflated with air of a corresponding volume through the air pump 36 to expand the air bag 35 and extrude the rubber pipette 37, so that reagent with a specified volume can be sucked, 5ML of filtrate can be sucked from the filter box 7 through the sliding of the sliding block 30 and transferred into the centrifuge tube on the centrifuge tube seat 26, a new rubber pipette 37 can be clamped through the first chuck 33 on the right, the PBS solution can be sucked from the reagent rack 39 and added into the solution in the centrifuge tube to 10ML, then the worker can place the extracted cell solution into the centrifuge for centrifugation at 1000RPM for 5 minutes, place the cell solution into the centrifuge tube seat 26 again after centrifugation is finished, add the PBS solution into the centrifuge tube again through the rubber pipette 37, and simultaneously, the connecting shaft 25 and the centrifuge tube seat 26 can be driven by the fifth motor 24 to rotate and oscillate, so as to fully dissolve the reagent, then a new rubber head suction pipe 37 is clamped by the first clamping head 33 to suck the supernatant in the centrifugal tube and extrude the supernatant into the waste liquid box 38 for storage, finally the new rubber head suction pipe 37 is used to suck 5ML of culture medium at the reagent rack 39 to be added into the centrifugal tube, the solution is transferred into a culture bottle on the upper surface of the culture bottle seat 27, and the culture bottle can be placed into an O2 culture box for culture after the whole cell solution is treated.

In summary, the experimental device for promoting differentiation and growth of epidermal stem cells is used by firstly placing the collected tissue sample into the culture dish 16, adding 2ML of 0.25% trypsin for digestion for 5-7min, then adding 1ML of culture medium to stop digestion, and then placing the pretreated culture dish 16 on the upper surface of the second bearing plate 18 for subsequent filtration treatment, wherein the first electric telescopic rod 9 is arranged at the end of the bearing rod 8 on the upper surface of the first bearing plate 4, so that the vacuum chuck 11 at the lower end of the first bearing plate is favorable for driving the first electric telescopic rod to adsorb the filter box 7 at the storage groove 6 on the upper surface of the first bearing plate 4 and lift the filter box upwards, and the first bearing plate 4 can be rotated and adjusted by the driving of the first motor 3, so that the hole site 5 formed on the upper surface of the first electric telescopic rod 9 is located below the filter box 7, and the filter box 7 adsorbed at the lower end of the first electric telescopic rod 9 is pushed by the first electric telescopic rod 5 to descend to the culture dish 16 on the upper surface of the second (ii) a

Then the filter box 7 pushed by the first electric telescopic rod 9 can be connected with the upper end of a culture dish 16 in a clamping way through a connector 12 connected with the lower end of the filter box, meanwhile, the vacuum sucker 11 loses the adsorption force with the filter box 7 and is driven by the first electric telescopic rod 9 to separate from the filter box 7, the pneumatic chuck 22 on the second loading plate 18 is rotated to the upper side of the filter box 7 by the third motor 19, and the pneumatic chuck 22 contacts the upper surface of the filter box 7 to carry out limit clamping through the contraction of the second air cylinder 20, because the upper end of the second cylinder 20 is connected with the fourth motor 21, the fourth motor 21 can conveniently drive the culture dish 16 on the upper surface of the second bearing plate 18 and the filter box 7 to rotate together for 180 degrees, so that the cell tissue inside the culture dish 16 is filtered by the filter screen 14 in the connector 12, and the filtered filtrate flows into the filter box 7 for storage;

along with the filtration of the cell tissue, the vacuum chuck 11 above the cell tissue descends again and adsorbs the upper surface of the culture dish 16, the second motor 10 drives the vacuum chuck 11 and the culture dish 16 adsorbed by the vacuum chuck to rotate, the rotating culture dish 16 drives the connector 12 clamped with the rotating culture dish 16 to unscrew and separate from the upper end of the filter box 7, the filtered culture dish 16 is placed in the storage groove 6 on the upper surface of the first bearing plate 4 through the ascending of the first electric telescopic rod 9, and at the moment, filtered cell fluid is obtained in the filter box 7;

the filtered filter box 7 is pushed by the first cylinder 17 to extend to the position below the first chuck 33 to be transported, the first chuck 33 can move downwards under the drive of the second electric telescopic rod 31 and clamp a clean glue head suction pipe 37 below under the drive of the bidirectional cylinder 32, the air bag 35 arranged in the first chuck 33 can be filled with corresponding volume of air through the air pump 36 so as to expand the air bag 35 and extrude the glue head suction pipe 37, reagent suction with a specified volume can be performed, 5ML of filtrate can be sucked from the filter box 7 through the sliding of the sliding block 30 and transferred to a centrifugal tube on the centrifugal tube seat 26, a new glue head suction pipe 37 can be clamped through the first chuck 33 at the right side, PBS solution is sucked from the reagent rack 39 and added to 10ML in the centrifugal tube, and then a worker can place the extracted cell solution in the centrifugal machine to centrifuge at 1000RPM for 5 minutes, after centrifugation, putting the sample into the centrifugal test tube seat 26 again;

finally, PBS solution is added into the centrifugal test tube again through the rubber head suction tube 37, the connecting shaft 25 and the centrifugal test tube seat 26 can be driven by the fifth motor 24 to rotate and vibrate to fully dissolve the reagent, then the new rubber head suction tube 37 is clamped by the first clamping head 33 to suck out the supernatant in the centrifugal tube and extrude the supernatant into the waste liquid box 38 for storage, the new rubber head suction tube 37 is used to suck 5ML culture medium at the reagent rack 39 to be added into the centrifugal tube, the solution is transferred into a culture bottle on the upper surface of the culture bottle seat 27, and the whole cell solution can be placed into a 37 ℃ O2 culture box for static culture after being treated.

The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.

Claims

1. An experimental device for promoting differentiation and growth of epidermal stem cells, which comprises a base (1) and a second support rod (23), and is characterized in that: the upper surface of the base (1) is fixed with a first supporting rod (2), a first motor (3) is arranged at the upper end of the first supporting rod (2), the upper end of the first motor (3) is connected with a first bearing plate (4), a hole site (5) is arranged at the edge of the upper surface of the first bearing plate (4), object placing grooves (6) are arranged at intervals of the hole sites (5), a filter box (7) is arranged on the upper surface of the object placing grooves (6), the middle part of the upper surface of the first bearing plate (4) is connected with a bearing rod (8), a first electric telescopic rod (9) is arranged at the tail end of the bearing rod (8), the lower end of the first electric telescopic rod (9) is connected with a second motor (10), the lower end of the second motor (10) is connected with a vacuum chuck (11), the open end of the filter box (7) is connected with a connector (12), and a thread, a filter screen (14) is fixed below the thread groove (13), a clamping groove (15) is formed in the inner wall of the connector (12), the clamping groove (15) is located below the thread groove (13), a culture dish (16) is attached to the inner wall of the clamping groove (15), a first air cylinder (17) is arranged inside the first supporting rod (2), the tail end of the first air cylinder (17) is connected with a second supporting plate (18), a third motor (19) is arranged inside the tail end of the second supporting plate (18), the upper end of the third motor (19) is connected with a second air cylinder (20), the upper end of the second air cylinder (20) is connected with a fourth motor (21), the tail end of the fourth motor (21) is connected with a pneumatic chuck (22), a second supporting rod (23) is arranged at the right side of the first supporting rod (2), a fifth motor (24) is arranged inside the second supporting rod (23), a connecting shaft (25) is connected to the upper end of the fifth motor (24, a centrifugal test tube seat (26) is fixed on the outer wall of one side of the connecting shaft (25), a culture bottle seat (27) is fixed on the outer wall of the other side of the connecting shaft (25), a sixth motor (28) is arranged inside the tail end of the second supporting rod (23), the upper end of the sixth motor (28) is connected with a moving rod (29), the outer wall of the moving rod (29) is connected with a sliding block (30), a second electric telescopic rod (31) is fixed on the outer wall of the sliding block (30), the lower end of the second electric telescopic rod (31) is connected with a bidirectional cylinder (32), the tail end of the bidirectional cylinder (32) is connected with a first chuck (33), an inner cavity (34) is formed inside the first chuck (33), an air bag (35) is arranged on the inner wall of the inner cavity (34), the upper end of the air bag (35) is connected with an air pump (36), a rubber head suction tube (37) is arranged on, and a reagent rack (39) is arranged on the right side of the second support rod (23).

2. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: first loading board (4) constitute rotating-structure through between first motor (3) and first bracing piece (2), and first loading board (4) upper surface encircles and distributes and have hole site (5), and hole site (5) and put thing groove (6) crisscross distribution moreover.

3. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the upper surface of the storage groove (6) is attached to the lower surface of the filter box (7), and the size of the outer opening of the filter box (7) is smaller than that of the inner opening of the hole site (5).

4. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: vacuum chuck (11) constitute elevation structure between through first electric telescopic handle (9) and carrier bar (8), and vacuum chuck (11) constitute revolution mechanic through between second motor (10) and first electric telescopic handle (9), are parallel distribution moreover between vacuum chuck (11) and filter box (7).

5. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the filter box (7) forms a movable structure between the thread groove (13) and the connector (12), the filter box (7) forms a clamping structure between the clamping groove (15) and the culture dish (16), and the size of the inner opening of the clamping groove (15) is matched with the size of the outer opening of the filter screen (14).

6. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the second bearing plate (18) forms a movable structure with the first supporting rod (2) through the first cylinder (17), and the second bearing plate (18) is movably connected with the pneumatic chuck (22).

7. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the movable rod (29) and the second support rod (23) form an adjustable structure through a sixth motor (28), the vertical center line of the movable rod (29) is overlapped with the vertical center line of the connecting shaft (25), and the connecting shaft (25) forms a rotating structure through a fifth motor (24).

8. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the first chuck (33) forms a sliding structure through the sliding block (30) and the moving rod (29), the first chuck (33) forms a movable structure through the bidirectional cylinder (32) and the sliding block (30), and the first chuck (33) is symmetrically distributed about the vertical center line of the bidirectional cylinder (32).

9. The experimental device for promoting differentiation and growth of epidermal stem cells as claimed in claim 1, wherein: the size of the inner opening of the inner cavity (34) is matched with the size of the outer opening of the upper end of the rubber head suction pipe (37), and the outer surface of the rubber head suction pipe (37) is attached to the outer surface of the air bag (35).