CN107058084A - SVF automatic cell separating devices and its method - Google Patents
SVF automatic cell separating devices and its method Download PDFInfo
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- CN107058084A CN107058084A CN201710088211.9A CN201710088211A CN107058084A CN 107058084 A CN107058084 A CN 107058084A CN 201710088211 A CN201710088211 A CN 201710088211A CN 107058084 A CN107058084 A CN 107058084A
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- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 239000002699 waste material Substances 0.000 claims abstract description 16
- 210000000577 adipose tissue Anatomy 0.000 claims description 21
- 239000002504 physiological saline solution Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 10
- 239000012528 membrane Substances 0.000 claims description 7
- 238000003756 stirring Methods 0.000 claims description 7
- 238000005406 washing Methods 0.000 claims description 7
- 239000008280 blood Substances 0.000 claims description 4
- 210000004369 blood Anatomy 0.000 claims description 4
- 238000012790 confirmation Methods 0.000 claims description 4
- 238000007443 liposuction Methods 0.000 claims description 4
- 230000002572 peristaltic effect Effects 0.000 claims description 4
- 239000000725 suspension Substances 0.000 claims description 4
- 102000008186 Collagen Human genes 0.000 claims description 3
- 108010035532 Collagen Proteins 0.000 claims description 3
- 102000004190 Enzymes Human genes 0.000 claims description 3
- 108090000790 Enzymes Proteins 0.000 claims description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 239000012267 brine Substances 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 229920001436 collagen Polymers 0.000 claims description 3
- 239000000463 material Substances 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 238000010079 rubber tapping Methods 0.000 claims description 3
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims 1
- 230000001954 sterilising effect Effects 0.000 claims 1
- 238000004659 sterilization and disinfection Methods 0.000 claims 1
- 238000010200 validation analysis Methods 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 28
- 238000000926 separation method Methods 0.000 abstract description 15
- 210000000130 stem cell Anatomy 0.000 abstract description 12
- 238000000605 extraction Methods 0.000 abstract description 8
- 230000000694 effects Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 2
- 238000001914 filtration Methods 0.000 description 5
- 210000001519 tissue Anatomy 0.000 description 4
- 230000008929 regeneration Effects 0.000 description 3
- 238000011069 regeneration method Methods 0.000 description 3
- 239000012266 salt solution Substances 0.000 description 3
- 210000002901 mesenchymal stem cell Anatomy 0.000 description 2
- 230000035479 physiological effects, processes and functions Effects 0.000 description 2
- 208000017667 Chronic Disease Diseases 0.000 description 1
- 210000004322 M2 macrophage Anatomy 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 210000001789 adipocyte Anatomy 0.000 description 1
- 230000005779 cell damage Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 210000002808 connective tissue Anatomy 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 210000002889 endothelial cell Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000002950 fibroblast Anatomy 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 210000003716 mesoderm Anatomy 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 210000003668 pericyte Anatomy 0.000 description 1
- 210000000229 preadipocyte Anatomy 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000001172 regenerating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 210000000329 smooth muscle myocyte Anatomy 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 230000037314 wound repair Effects 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/48—Automatic or computerized control
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/02—Means for pre-treatment of biological substances by mechanical forces; Stirring; Trituration; Comminuting
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M45/00—Means for pre-treatment of biological substances
- C12M45/04—Phase separators; Separation of non fermentable material; Fractionation
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
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- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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Abstract
The invention discloses a kind of SVF automatic cell separating devices, including stand, liquid feeding component, main body tank, filter, power set are installed on stand, liquid feeding component is connected with main body tank conduit, main body tank point two-way conduit connecting power device, conduit is directly connected to after power set connect collector all the way, and another road conduit connecting power device after filter reconnects waste liquid cylinder.The device ensure that fully automatically collects SVF cells under whole process closing, aseptic condition.The apparatus structure is reasonable in design, simple and easy to use, and separation process is controllable, greatlys save disengaging time, improves separative efficiency, it is ensured that fat stem cell activity, separation rate is high.Accordingly, inventor has also set up corresponding application method, and using the automatic separating apparatus and its method, the management and industrialization of the extensive separation and Extraction of SVF cells can be achieved.
Description
Technical field
The invention belongs to stem cell separation and extraction technology field, more particularly to a kind of SVF automatic cell separating devices and its
Method.
Background technology
Adipose tissue turns into the cell derived that one haves a great attraction in organizational project and regenerative medicine, because it
Rich in stem/progenitor cells group and also can easily collect.From body fat draw thing separation original cuiture obtain it is thin
Born of the same parents group is referred to as adipose-derived vascular stroma part (SVF).SVF in adipose tissue contains different mesenchyma ancestral/population of stem cells
Than, such as except fatty ancestral/stem cell also has Preadipocyte, endothelial cell, smooth muscle cell, fibroblast, T cell,
Pericyte and M2 macrophages.By fat stem cell isolated SVF through induction, can easily extend in vitro and
The potential cell for being divided into different pedigrees, has in multiple regeneration fields such as wound repair, regeneration, chronic disease treatments
Extensive potential applicability in clinical practice.Researcher is by the method for enzymic digestion, from abdominoplasty or the discarded fat of lipsuction
SVF is separated in tissue.To SVF separation and Extractions cell is applied into reengineer on a large scale, then related organization's regeneration is needed badly
Cell is automatically separated instrument, and fat mesenchymal stem cell can be made to apply from phase of basic research, and really application to clinic benefits disease
Suffer from.
However, now in SVF cell separations extractive technique, in open separation and Extraction environment, easily causing pollution；And
Complex process needs more instrument, and extraction step is cumbersome and uncontrollable, such as adipose tissue needs artificial chopping, is not easy to face
Medical personnel use on bed.In addition, traditional fat stem cell separate mode is to carry out postdigestive tissue suspension with filter screen
Filtering, connective tissue gradually blocks filter opening in filter process, causes the slow time lengthening of the rate of filtration, have impact on primary cell
Activity.
The content of the invention
Divide automatically the technical problem to be solved in the present invention is to provide a kind of automatic, efficient, pollution-free, controllable SVF cells
From devices and methods therefor, in order to realize the management and industrialization of the extensive separation and Extraction of SVF cells.
In order to solve the above technical problems, the present invention uses following technical scheme：
Liquid feeding component is installed on SVF automatic cell separating devices, including stand, stand, it is main body tank, filter, dynamic
Power apparatus, liquid feeding component is connected with main body tank conduit, and conduit connects filter and power set to main body tank successively, and conduit is driven
Power apparatus comes out a point two-way, all the way conduit connection collector, and another road conduit connects waste liquid cylinder.
Cylinder controlling switch is equipped between liquid feeding component and main body tank, between filter and power set.
Liquid feeding component includes cuboid fixed block, and fixed block or so respectively has 1 hook, and hook one hangs 3 bags of 500ml physiology
Salt solution, 1 bag of 200ml physiological saline containing sterilized confirmation clostridiopetidase A of the suspension of hook two, each physiological saline passes through band gas respectively
The connecting conduit of cylinder controlling switch is connected with the cover connecting hole of main body tank.
There are two connecting holes and fatty well on the cover of main body tank, two connecting holes are respectively with linking up with one and hook two
On normal saline bag be connected；Pipeline of the fatty well directly with liposuction instrument is connected；The tank body center of main body tank has
Agitator, agitator is made up of multiple paddles；Stirrer bottom connects motor by coupler；The tank base of main body tank has
Liquid outlet connects filter.
Power set are peristaltic pump.
Filter is the obturator for including filter core, and its top connects main body tank through conduit, and filter core is many
The filter membrane of road different pore size size.
Collector is the obturator for including central filter core, and central filter core has 0.45 μm of filter membrane.
Above-mentioned SVF automatic cell separating devices also include console, console connects and controls liquid feeding component, agitator,
Power set and cylinder controlling switch.
The application method of above-mentioned SVF automatic cell separating devices, 200ml adipose tissues are put into after main body tank, closed master
Body tank, opens console, is first turned on connecting the cylinder controlling switch of 3 bags of 500ml physiological saline, and each tapping 500ml starts
Agitator stirs adipose tissue to clean the materials such as the blood in adipose tissue；After washing 1 minute, automatic start power set are beaten
The cylinder controlling switch of connection waste liquid cylinder is opened, waste liquid is discharged, repeated washing three times, then, console are opened containing sterilized true
Recognize the cylinder controlling switch of the 200ml physiological saline of clostridiopetidase A, be put into clostridiopetidase A, be stirred at room temperature 30 minutes；After having stirred,
Stand 3 minutes；Start power set, lower floor's solution containing SVF is pumped into collector；The solution centrifugal obtained in collector
Precipitated, then the collagen enzyme component of residual is removed with centrifugation after brine, obtain pure SVF.
The problem of lacking relevant extraction separation equipment for large-scale application SVF cells, it is thin that inventor devises a kind of SVF
Liquid feeding component, main body tank, filter, power set, liquid feeding group are installed on born of the same parents' automatic separating apparatus, including stand, stand
Part is connected with main body tank conduit, main body tank conduit connection filter and power set successively, and conduit comes out point from power set
Two-way, conduit connection collector all the way, another road conduit connects waste liquid cylinder.The device ensure that in whole process closing, sterile bar
SVF cells are fully automatically collected under part.Wherein, disposable main body tank avoids reusing, and prevents cross pollution；Agitator is used
The stirring of multiple paddles, save and manually shred, it is to avoid shearing machine inequality of exerting oneself causes a large amount of cellular damages；Adipose tissue hangs
Liquid is purified through Multi-channel filtering film and filtered, and finally collects pure SVF cells；In addition, whole device is by power set and gas
Cylinder controlling switch, console management, can be achieved controllable, full-automatic.The apparatus structure is reasonable in design, simple and easy to use, separation
Process control, greatlys save disengaging time, improves separative efficiency, it is ensured that fat stem cell activity, separation rate is high.According to
This, inventor has also set up corresponding application method, using the automatic separating apparatus and its method, SVF cells can be achieved extensive
The management and industrialization of separation and Extraction.
Brief description of the drawings
Fig. 1 is the structural representation of SVF automatic cell separating devices of the present invention.
Fig. 2 is the structural representation of filter in SVF automatic cell separating devices of the present invention.
Fig. 3 is the structural representation of main body tank in SVF automatic cell separating devices of the present invention.
Fig. 4 is the structural representation of agitator in SVF automatic cell separating devices of the present invention.
Fig. 5 is the structural representation of collector in SVF automatic cell separating devices of the present invention.
In figure：1 liquid feeding component, 2 one cylinder controlling switches of hook, 3 two cylinder controlling switches of hook, 4 main body tanks, 5 stirrings
Device, 6 filters, 7 consoles, 8 power set, 9 waste liquid cylinders, 10 collectors.
Embodiment
First, basic structure and its function
As shown in Fig. 1 to 5, SVF automatic cell separating devices of the invention mainly include on stand and console 7, stand
Liquid feeding component 1, main body tank 4, filter 6, power set 8 are installed, liquid feeding component is connected with main body tank conduit, main body tank according to
Secondary conduit connection filter and power set, conduit come out a point two-way from power set, and conduit connection collector 10 is used all the way
In collecting SVF solution, another road conduit connection waste liquid cylinder is used to collect waste liquid.Between liquid feeding component and main body tank, filter
Cylinder controlling switch is equipped between power set.Console connects and controls liquid feeding component, agitator 5, power set and
Cylinder controlling switch.Wherein,
Liquid feeding component includes cuboid fixed block, and fixed block or so respectively has 1 hook, and hook one hangs 3 bags of 500ml physiology
Salt solution, 200ml physiological saline (clostridiopetidase A content be 0.1%) of 1 bag of the suspension of hook two containing sterilized confirmation clostridiopetidase A, each life
Reason salt solution is connected by the connecting conduit with cylinder controlling switch with the cover connecting hole of main body tank respectively.
There are two connecting holes and fatty well on the cover of main body tank, two connecting holes are respectively with linking up with one and hook two
On normal saline bag be connected；Fatty well can be directly with liposuction instrument pipeline be connected；The tank body center of main body tank
There is agitator, agitator is made up of multiple paddles；Stirrer bottom connects motor by coupler；The tank base of main body tank
There is liquid outlet to connect filter.
Power set are peristaltic pump, and peristaltic pump by PLC controls and provides power for separation solution.
Filter is the obturator for including filter core, and its top connects main body tank through conduit, and filter core is many
(80~120 μm, generally four layers, the filter sizes of each layer are respectively 200 μm to the filter membrane of road different pore size size, 150 μm, 100
μm, 50 μm, for filtering the indigested lipochondrion of bulk and intercepting different size of fat cell).Collector is in including
The obturator of filter core is entreated, there is central filter core 0.45 μm of filter membrane to be used to finally collect cell mesh sheet, fat stem cell and interior
Chrotoplast can be eventually restricted from by former road filter membranes at last one, and water and clostridiopetidase A are effectively realized by discharge
SVF filtering and collection in adipose tissue, can collect a large amount of SVF for digesting acquisition for clinic.
Console controls the operation of single unit system.Processing unit of the control feature comprising inside and it can control to be permitted
The related software program of multiprocessing function, can be automated manipulation.System convergence power set, pass through the journey write in advance
Sequence, controls the running of whole separator.Collector connecting power device, power set are by providing power and in programme-control
The cylinder controlling switch of lower manipulation output waste liquid and SVF solution lines, can be pumped into waste liquid cylinder by waste liquid respectively and contain SVF
Solution be pumped into collector.
2nd, application method
200ml or so adipose tissue is put into after main body tank, closed main body tank, opens console, be first turned on connection 3
The cylinder controlling switch 2 of hook one of bag 500ml physiological saline, each tapping 500ml starts agitator stirring adipose tissue to wash
The materials such as the blood in net adipose tissue；After washing 1 minute, automatic start power set open the cylinder control of connection waste liquid cylinder
Switch, discharges waste liquid, and required adipose tissue is stopped by filter core, repeated washing three times, it is ensured that adipose tissue washing is dry
Only；Then, console opens the cylinder controlling switch 3 of hook two of the 200ml physiological saline containing sterilized confirmation clostridiopetidase A, puts
Enter clostridiopetidase A, be stirred at room temperature 30 minutes so that clostridiopetidase A fully digests adipose tissue so that the fat mesenchymal of the inside is done
The cell components such as cell are discharged from lipochondrion；After having stirred, 3 minutes are stood, bulky grain fat swims in upper strata, contains
There is SVF liquid in lower floor；Start power set, lower floor's solution containing SVF is pumped into collector；Obtained about in collector
200ml solution, is dispensed into 50ml centrifuge tubes, and centrifugation precipitate, then with brine once after, removal is centrifuged again
The collagen enzyme component of residual, obtains pure SVF, for clinic.
In actual use, according to the mode of addition adipose tissue, the cover of main body tank can be opened manually directly by monoblock fat
Fat tissue add main body tank, or by well be connected with the pipeline of liposuction instrument realization automatically add adipose tissue.
Experiment shows, using the present invention and its method, can probably be extracted from 100ml adipose tissues and obtain 1X106Between
Mesenchymal stem cells, extract the quantity that separation rate is significantly larger than mescenchymal stem cell in same volume marrow blood, can meet clinical tissue
Regenerate the requirement to mescenchymal stem cell quantity.
Claims (9)
1. a kind of SVF automatic cell separating devices, including stand, it is characterised in that be provided with stand liquid feeding component, main body tank,
Filter, power set, liquid feeding component are connected with main body tank conduit, and conduit connection filter and power are filled main body tank successively
Put, conduit comes out a point two-way from power set, all the way conduit connection collector, another road conduit connects waste liquid cylinder.
2. SVF automatic cell separating devices according to claim 1, it is characterised in that：The liquid feeding component and main body tank
Between, between filter and power set be equipped with cylinder controlling switch.
3. SVF automatic cell separating devices according to claim 2, it is characterised in that：The liquid feeding component includes rectangular
Body fixed block, fixed block or so respectively has 1 hook, and hook one hangs 3 bags of 500ml physiological saline, and 1 bag of the suspension of hook two contains warp
The 200ml physiological saline of sterilization validation clostridiopetidase A, each physiological saline passes through the connecting conduit with cylinder controlling switch and master respectively
The cover connecting hole of body tank is connected.
4. SVF automatic cell separating devices according to claim 2, it is characterised in that：Have on the cover of the main body tank
Two connecting holes and fatty well, two connecting holes are connected with the normal saline bag on hook one and hook two respectively；Fat
Pipeline of the fat well directly with liposuction instrument is connected；There is agitator in the tank body center of main body tank, and agitator is by multiple stirrings
Leaf is constituted；Stirrer bottom connects motor by coupler；The tank base of main body tank has liquid outlet to connect filter.
5. SVF automatic cell separating devices according to claim 2, it is characterised in that：The power set are peristaltic pump.
6. SVF automatic cell separating devices according to claim 2, it is characterised in that：The filter is to include
The obturator of filter core is filtered, its top connects main body tank through conduit, and filter core is the filter membrane of multiple tracks different pore size size.
7. SVF automatic cell separating devices according to claim 2, it is characterised in that：The collector is to include center
The obturator of filter core, central filter core has 0.45 μm of filter membrane.
8. SVF automatic cell separating devices according to claim 2, it is characterised in that also including console, console connects
Connect and control liquid feeding component, agitator, power set and cylinder controlling switch.
9. the application method of SVF automatic cell separating devices described in claim 2, it is characterised in that：200ml adipose tissues are put
Enter after main body tank, closed main body tank, open console, be first turned on connecting the cylinder controlling switch of 3 bags of 500ml physiological saline,
Each tapping 500ml, starts agitator stirring adipose tissue to clean the materials such as the blood in adipose tissue；After washing 1 minute,
Automatic start power set open the cylinder controlling switch of connection waste liquid cylinder, discharge waste liquid, and then repeated washing three times, controls
Platform opens the cylinder controlling switch of the 200ml physiological saline containing sterilized confirmation clostridiopetidase A, is put into clostridiopetidase A, stirs at room temperature
Mix 30 minutes；After having stirred, 3 minutes are stood；Start power set, lower floor's solution containing SVF is pumped into collector；Collect
The solution centrifugal obtained in device is precipitated, then removes the collagen enzyme component of residual with centrifugation after brine, is obtained pure
Net SVF.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710088211.9A CN107058084A (en) | 2017-02-17 | 2017-02-17 | SVF automatic cell separating devices and its method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710088211.9A CN107058084A (en) | 2017-02-17 | 2017-02-17 | SVF automatic cell separating devices and its method |
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| CN201710088211.9A Pending CN107058084A (en) | 2017-02-17 | 2017-02-17 | SVF automatic cell separating devices and its method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485916A (en) * | 2018-05-14 | 2018-09-04 | 湖州韩汇进出口有限公司 | It is a kind of to extract stem cell extraction equipment small to cell contamination soon |
| CN112940914A (en) * | 2021-01-29 | 2021-06-11 | 赵彬 | Experimental device for promote differentiation of epidermal stem cells and growth |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150004702A1 (en) * | 2011-08-29 | 2015-01-01 | Stempeutics Research Private Limited | System for isolating stromal vascular fraction (svf) cells from the adipose tissue and a method thereof |
| CN106068124A (en) * | 2014-02-28 | 2016-11-02 | Bdbc科学公司 | A kind of system for tissue manipulation |
| CN206654917U (en) * | 2017-02-17 | 2017-11-21 | 广西医科大学 | SVF automatic cell separating devices |
| CN215288820U (en) * | 2021-02-07 | 2021-12-24 | 江苏普瑞康生物医药科技有限公司 | Adipose-derived stem cell extraction device |
-
2017
- 2017-02-17 CN CN201710088211.9A patent/CN107058084A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150004702A1 (en) * | 2011-08-29 | 2015-01-01 | Stempeutics Research Private Limited | System for isolating stromal vascular fraction (svf) cells from the adipose tissue and a method thereof |
| CN106068124A (en) * | 2014-02-28 | 2016-11-02 | Bdbc科学公司 | A kind of system for tissue manipulation |
| CN206654917U (en) * | 2017-02-17 | 2017-11-21 | 广西医科大学 | SVF automatic cell separating devices |
| CN215288820U (en) * | 2021-02-07 | 2021-12-24 | 江苏普瑞康生物医药科技有限公司 | Adipose-derived stem cell extraction device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108485916A (en) * | 2018-05-14 | 2018-09-04 | 湖州韩汇进出口有限公司 | It is a kind of to extract stem cell extraction equipment small to cell contamination soon |
| CN108485916B (en) * | 2018-05-14 | 2021-08-17 | 中南大学湘雅二医院 | Stem cell extraction equipment with rapid extraction and small cell pollution |
| CN112940914A (en) * | 2021-01-29 | 2021-06-11 | 赵彬 | Experimental device for promote differentiation of epidermal stem cells and growth |
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Application publication date: 20170818 |