CN108485916A - It is a kind of to extract stem cell extraction equipment small to cell contamination soon - Google Patents
It is a kind of to extract stem cell extraction equipment small to cell contamination soon Download PDFInfo
- Publication number
- CN108485916A CN108485916A CN201810455773.7A CN201810455773A CN108485916A CN 108485916 A CN108485916 A CN 108485916A CN 201810455773 A CN201810455773 A CN 201810455773A CN 108485916 A CN108485916 A CN 108485916A
- Authority
- CN
- China
- Prior art keywords
- retort
- syringe
- electric telescopic
- telescopic rod
- stem cell
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Sustainable Development (AREA)
- Clinical Laboratory Science (AREA)
- Cell Biology (AREA)
- Developmental Biology & Embryology (AREA)
- Mechanical Engineering (AREA)
- Rheumatology (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Stem cell extraction equipment small to cell contamination soon is extracted the invention discloses a kind of, including retort, the retort is cylindrical can, strainer is fixedly installed in retort bosom position, six pieces of partition boards are set between the strainer and the inner wall of retort, and six pieces of partition boards are by six decile of space between strainer and retort, and mounting hole is arranged in the corresponding retort side wall lower ends in space being divided between strainer and retort, cooperation installation bend pipe at the mounting hole of the reaction pot sidewall, this extraction soon the stem cell extraction equipment small to cell contamination using retort and syringe combination, adipose tissue is set constantly to move, quick emulsification, promote cell separation convenient for extraction, and merge centrifugal action, stem cell is set quickly to be detached with other body fluid, and cell whole process is in enclosed environment, reduce contaminated probability.
Description
Technical field
The present invention relates to cell extraction equipment technical field, the stem cell small to cell contamination carries soon for specially a kind of extraction
Taking equipment.
Background technology
Autologous fat transplantation is a kind of generally acknowledged therapeutic modality for having extensive clinical indication, including Soft-tissue operation increasing
Greatly, improve radiotherapy and post-traumatic tissue regions and a variety of cosmetic applications.From adipose tissue stromal vascular cell mass
Autologous vein correlation progenitor cells are extracted, are added it in fat transfer tissue, to improve the effect and reliability of fat transfer,
This is the theme of a new technology and many clinical researches.Involve the safety of the fat transfer of data logging cellular modalities
Property and validity, this enhance detach demand of the SVF cells to instrument in operating room from liposuction aspirates.Currently on the market may be used
A variety of manual, semi-automatic, automatic SVF piece-rate systems are bought, are placed on the key position of operating room, for extracting SVF to increase
Stuffing fat transplantation effect.Adipose tissue SVF cells piece-rate system has MultiStation, CHA-Station of South Korea in the market
With the equipment such as Lipo-kit with MaxStem, the CRS Syetem in the U.S., substantially consist of the following components：Centrifuge,
Shake constant temperature machine, the fatty machine of sucking, sterile bag, experiment tube etc..The conventional method of extraction is that the fat of extraction is injected into experiment
Pipe, is then placed in centrifuge and centrifuges, and after clostridiopetidase A is added, then is put on vibrations constant temperature machine and is mixed, neutralizes, washes
It washs, a series of processes such as cell filtration.The equipment that above-mentioned adipose tissue SVF cell separation processes are related to is relatively more, consumption when
Between it is longer, be also required to complete for 40 minutes wherein most fast CHA-Station equipment extracts the time, and in the process of extraction
Middle cell is bigger by the probability of risk.
Invention content
The technical problem to be solved by the present invention is to overcome the existing defects, and it is small to cell contamination dry soon to provide a kind of extraction
Cell extraction equipment makes adipose tissue constantly move using retort and syringe combination, quick emulsification, promotes cell point
From convenient for extraction, and centrifugal action is merged, stem cell is enable quickly to be detached with other body fluid, and cell whole process is in envelope
Closed loop border reduces contaminated probability, can effectively solve the problems in background technology.
To achieve the above object, the present invention provides the following technical solutions：It is a kind of to extract stem cell small to cell contamination soon
Extraction equipment, including retort, the retort are cylindrical can, and strainer, institute is fixedly installed in retort bosom position
State between strainer and the inner wall of retort six pieces of partition boards of setting, and six pieces of partition boards are by the space six etc. between strainer and retort
Point, and mounting hole, the retort side is arranged in the corresponding retort side wall lower ends in space being divided between strainer and retort
Cooperation installation bend pipe at the mounting hole of wall, the bend pipe outer end cooperation installation solenoid valve, the solenoid valve end is matched with syringe two
Installation is closed, the centre position of the retort upper end is arranged mounting hole, solenoid valve is arranged at the mounting hole of the retort upper end,
The solenoid valve of the retort upper end is connected to syringe one, and the syringe one is identical with two structure of syringe, the syringe one and needle
Cylinder two includes injection tube and piston rod, and the injection tube and piston rod end are respectively provided with limiting plate, the injection tube and piston rod
Coordinate chuck for installing outside the limiting plate of end, the chuck is installed with movable plate cooperation, the injection tube of the syringe one and
The corresponding movable plate in piston rod end is connect by electric telescopic rod two and electric telescopic rod one with workbench respectively, the syringe
The two corresponding movable plate of injection tube and piston rod is connect by electric telescopic rod three and electric telescopic rod four with fixed plate respectively,
The fixed plate is fixedly mounted with workbench, and pallet is arranged below the retort, ultrasonic wave is fixedly installed below the pallet
Generator, the pallet are installed with workbench cooperation, and the workbench lower end is fixedly connected by connecting rod with turntable, described turn
Input shaft, the input shaft of the turntable and the output axis connection of motor are fixedly installed at the axial location of disk lower end, the motor is logical
It crosses motor mount to be fixedly mounted with rack, the turntable lower end is installed by plane bearing and rack cooperation, the pallet
Internal engagement installs heating plate, the heating plate, solenoid valve, motor, electric telescopic rod one, electric telescopic rod two, electric expansion
Bar three and the input terminal of electric telescopic rod four are electrically connected with the output end of microcontroller, and the microcontroller is fixedly mounted with rack, institute
The input terminal for stating microcontroller is electrically connected with the output end of external power supply.
As a preferred technical solution of the present invention, temperature sensor is set, the temperature passes inside the retort
The output end of sensor is electrically connected with the input terminal of microcontroller.
As a preferred technical solution of the present invention, three pressing plates are set outside the retort, and three pressing plates around
The axis of retort is uniformly arranged.
As a preferred technical solution of the present invention, the pressing plate is L-shaped structure, and the centre position of pressing plate passes through hinge
Chain is connect with pallet, and the lower end of pressing plate is connect by electric telescopic rod five with pallet, the input terminal of the electric telescopic rod five
It is electrically connected with the output end of microcontroller.
As a preferred technical solution of the present invention, foam-rubber cushion is installed additional between the upper end and retort of the pressing plate, institute
The inner wall for stating foam-rubber cushion and pressing plate is fixedly mounted.
As a preferred technical solution of the present invention, connection is installed between the syringe one and syringe two and solenoid valve additional
Pipe is cooperation really up to the mark between the connecting tube and syringe one, syringe two and solenoid valve, and connecting tube is flexible rubber hose.
As a preferred technical solution of the present invention, the pallet is installed by connector and workbench cooperation, described
Connector is rubber block.
Compared with prior art, the beneficial effects of the invention are as follows：Small to cell contamination stem cell extraction is set soon for this extraction
It is standby so that adipose tissue is continued back and forth movement between syringe two and retort using syringe two is arranged around retort, accelerate fat
Fat tissue emulsifies, and is convenient for the separation and Extraction of stem cell.
This extraction soon the stem cell extraction equipment small to cell contamination using closed retort and syringe two as dry thin
Born of the same parents detach and the container of extraction, and entire extraction process adipose tissue is constantly in inside retort and syringe two, reduce cell with
The contact of external environment reduces the contaminated probability of stem cell.
Syringe one is arranged in the retort upper end of this extraction stem cell extraction equipment small to cell contamination soon, and syringe one is adopted
It is driven with electric telescopic rod one and electric telescopic rod two, realizes the automatic addition of reaction enzymes, while ensureing reaction speed, subtract
Few hand labor realizes automation separation and extraction.
The syringe one of the stem cell extraction equipment small to cell contamination and syringe two are all made of chuck and fix soon for this extraction, side
Just the dismounting of syringe one and syringe two, is conveniently replaceable, and the mode that can be used and replace syringe one and syringe two is used for multiple times, avoids handing over
Fork infection.
Supersonic generator is arranged inside small to cell contamination stem cell extraction equipment soon in this extraction, utilizes ultrasonic wave pair
Adipose tissue acts on, and so that adipose tissue is generated vibrations, promotes adipose tissue accelerated decomposition, promote the extraction rate of stem cell.
Description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is structure of the invention sectional view；
Fig. 3 is retort assembling schematic diagram of the present invention；
Fig. 4 is two assembling schematic diagram of syringe of the present invention；
Fig. 5 is retort schematic internal view of the present invention.
In figure：1 retort, 2 syringes one, 3 syringes two, 4 chucks, 5 movable plates, 6 workbench, 7 turntables, 8 racks, 9 electromagnetism
Valve, 10 bend pipes, 11 electric telescopic rods one, 12 electric telescopic rods two, 13 electric telescopic rods three, 14 electric telescopic rods four, 15 are fixed
Plate, 16 microcontrollers, 17 plane bearings, 18 motors, 19 connecting tubes, 20 strainers, 21 pressing plates, 22 foam-rubber cushions, 23 pallets, 24 ultrasonic waves
Generator, 25 electric telescopic rods five, 26 partition boards, 27 heating plates, 28 temperature sensors.
Specific implementation mode
Following will be combined with the drawings in the embodiments of the present invention, and technical solution in the embodiment of the present invention carries out clear, complete
Site preparation describes, it is clear that described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.It is based on
Embodiment in the present invention, it is obtained by those of ordinary skill in the art without making creative efforts every other
Embodiment shall fall within the protection scope of the present invention.
- 5 are please referred to Fig.1, the present invention provides a kind of technical solution：It is a kind of to extract stem cell extraction small to cell contamination soon
Equipment, including retort 1, retort 1 are cylindrical can, and 1 bosom position of retort is fixedly installed strainer 20, strainer 20 and anti-
It answers and six pieces of partition boards 26 is set between the inner wall of tank 1, and six pieces of partition boards 26 are by six decile of space between strainer 20 and retort 1,
And mounting hole, 1 side wall of retort is arranged in corresponding 1 side wall lower ends of retort in space being divided between strainer 20 and retort 1
Mounting hole at cooperation installation bend pipe 10, ensure that the adipose tissue of retort 1 bottom can be drawn by syringe 23, outside bend pipe 10
End cooperation installation solenoid valve 9,9 end of solenoid valve are installed with the cooperation of syringe 23, the centre position setting installation of 1 upper end of retort
Solenoid valve 9 is arranged at the mounting hole of 1 upper end of retort for hole, and the solenoid valve 9 of 1 upper end of retort is connected to syringe 1, syringe 1
Identical with 23 structure of syringe, syringe 1 and syringe 23 include injection tube and piston rod, and injection tube and piston rod end are respectively provided with
Chuck for installing 4, chuck 4 is coordinated to be installed with the cooperation of movable plate 5 outside the limiting plate of limiting plate, injection tube and piston rod end,
The corresponding movable plate of the injection tube of syringe 1 and piston rod end 5 passes through electric telescopic rod 2 12 and electric telescopic rod one respectively
11 connect with workbench 6, and the corresponding movable plate of the injection tube and piston rod of syringe 235 passes through 3 13 He of electric telescopic rod respectively
Electric telescopic rod 4 14 is connect with fixed plate 15, and fixed plate 15 is fixedly mounted with workbench 6, and pallet 23 is arranged in 1 lower section of retort,
Supersonic generator 24 is fixedly installed in 23 lower section of pallet, and pallet 23 is installed with the cooperation of workbench 6, and 6 lower end of workbench passes through connection
Bar is fixedly connected with turntable 7, and input shaft is fixedly installed at 7 lower end axial location of turntable, and the input shaft of turntable 7 is defeated with motor 18
Go out axis connection, motor 18 is fixedly mounted by motor mount and rack 8, and 7 lower end of turntable is matched by plane bearing 17 and rack 8
Installation is closed, the internal engagement of pallet 23 installs heating plate 27, heating plate 27, solenoid valve 9, motor 18, electric telescopic rod 1, electricity
The input terminal of dynamic telescopic rod 2 12, electric telescopic rod 3 13 and electric telescopic rod 4 14 is electrically connected with the output end of microcontroller 16,
Microcontroller 16 is fixedly mounted with rack 8, and the input terminal of microcontroller 16 is electrically connected with the output end of external power supply, inside retort 1
Temperature sensor 28 is set, and the output end of temperature sensor 8 is electrically connected with the input terminal of microcontroller 16, to 1 inside temperature of retort
Degree is monitored, and three pressing plates 21 are arranged in 1 outside of retort, and three pressing plates 21 are uniformly arranged around the axis of retort 1, will be anti-
Tank 1 is answered to be fixed with pallet 23, pressing plate 21 is L-shaped structure, and the centre position of pressing plate 21 is connect by hinge with pallet 23,
And the lower end of pressing plate 21 is connect by electric telescopic rod 5 25 with pallet 23, input terminal and the microcontroller 16 of electric telescopic rod 5 25
Output end electrical connection, install foam-rubber cushion 22 between the upper end and retort 1 of pressing plate 21 additional, the inner wall of foam-rubber cushion 22 and pressing plate 21 is solid
Dingan County fills, and prevents the pressure of pressing plate 21 is excessive from retort 1 being caused to deform, is installed additional between syringe 1 and syringe 23 and solenoid valve 9
Connecting tube 19 connect convenient for the syringe of different size with solenoid valve 9, connecting tube 19 and syringe 1, syringe 23 and solenoid valve 9 it
Between be cooperation really up to the mark, and connecting tube 19 is flexible rubber hose, and pallet 23 is installed by connector and the cooperation of workbench 6, and connector is
Rubber block, isolation pallet 23 and workbench 6 avoid the vibrations of pallet 23 from being transmitted to workbench 6, and microcontroller 16 controls heating plate
27, solenoid valve 9, motor 18, electric telescopic rod 1, electric telescopic rod 2 12, electric telescopic rod 3 13, electric telescopic rod four
14, electric telescopic rod 5 25 and the control mode of temperature sensor 28 are normal method in the prior art.
When in use：Reaction enzymes are packed into inside syringe 1, adipose tissue are packed into inside syringe 23, then by syringe
1 and syringe 23 and retort 1 correctly assemble, retort 1 is placed on 23 surface of pallet, microcontroller 16 controls electric expansion
Bar 5 25 acts, and pressing plate 21 is made to fix retort 1 and pallet 23, and then microcontroller 16 controls heating plate 27 and temperature sensor
28 collective effects make 1 inside of retort keep constant temperature, and microcontroller 16 controls electric telescopic rod 3 13 and electric telescopic rod simultaneously
4 14 actions, drive syringe 23 to work, and make adipose tissue in 1 inside persistent movement of syringe 23 and retort, while electronic stretching
Contracting bar 1 and electric telescopic rod 2 12 act, and control syringe 1 moves, and realizes the automatic addition of reaction enzymes, while ultrasonic wave
Generator 24 works, and adipose tissue itself is made to generate vibrations, accelerates adipose tissue decomposition and emulsification, and after the completion, solenoid valve 9 closes
It closes, motor 18 works, and drives workbench 6 to rotate, so that adipose tissue is centrifuged inside retort 1, by stem cell due to other groups
Knit separation.
Syringe 23 is arranged using 1 surrounding of retort in the present invention, and adipose tissue is made to continue between syringe 23 and retort 1
Back and forth movement accelerates adipose tissue emulsification, is convenient for the separation and Extraction of stem cell；This equipment uses closed retort 1 and syringe
23 containers for detaching and extracting as stem cell, entire extraction process adipose tissue are constantly in retort 1 and syringe 23
Portion reduces contact of the cell with external environment, reduces the contaminated probability of stem cell；And syringe 1 is arranged in 1 upper end of retort,
And syringe 1 is driven using electric telescopic rod 1 and electric telescopic rod 2 12, realizes the automatic addition of reaction enzymes, is being ensured instead
While answering speed, hand labor is reduced, realizes automation separation and extraction；It is solid that syringe 1 and syringe 23 are all made of chuck 4
It is fixed, facilitate the dismounting of syringe 1 and syringe 23, be conveniently replaceable, the side that can be used and replace syringe 1 and syringe 23 is used for multiple times
Formula avoids cross-infection；Supersonic generator 24 is set inside this equipment, adipose tissue is acted on using ultrasonic wave, makes fat
Tissue generates vibrations, promotes adipose tissue accelerated decomposition, promotes the extraction rate of stem cell.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
Understanding without departing from the principles and spirit of the present invention can carry out these embodiments a variety of variations, modification, replace
And modification, the scope of the present invention is defined by the appended.
Claims (8)
1. a kind of extracting stem cell extraction equipment small to cell contamination soon, including retort (1), it is characterised in that：It is described anti-
It is cylindrical can to answer tank (1), retort (1) the bosom position fixed setting strainer (20), the strainer (20) and reaction
Six pieces of partition boards (26) are set between the inner wall of tank (1), and six pieces of partition boards (26) are by the space between strainer (20) and retort (1)
Six deciles, and space corresponding retort (1) the side wall lower ends setting installation being divided between strainer (20) and retort (1)
Hole, cooperation installation bend pipe (10) at the mounting hole of retort (1) side wall, bend pipe (10) the outer end cooperation installation solenoid valve
(9), solenoid valve (9) end is installed with syringe two (3) cooperation, the centre position setting installation of retort (1) upper end
Hole, setting solenoid valve (9), the solenoid valve (9) and needle of retort (1) upper end at the mounting hole of retort (1) upper end
The connection of one (2) of cylinder, the syringe one (2) is identical with syringe two (3) structure, and the syringe one (2) and syringe two (3) include injection
Cylinder and piston rod, the injection tube and piston rod end are respectively provided with limiting plate, the limiting plate of the injection tube and piston rod end
External cooperation chuck for installing (4), the chuck (4) are installed with movable plate (5) cooperation, the injection tube of the syringe one (2) and
The corresponding movable plate in piston rod end (5) passes through electric telescopic rod two (12) and electric telescopic rod one (11) and workbench respectively
(6) it connects, the corresponding movable plate of injection tube and piston rod (5) of the syringe two (3) passes through electric telescopic rod three (13) respectively
It is connect with fixed plate (15) with electric telescopic rod four (14), the fixed plate (15) is fixedly mounted with workbench (6), the reaction
Setting pallet (23) below tank (1), fixed setting supersonic generator (24) below the pallet (23), the pallet (23) with
Workbench (6) cooperation installation, workbench (6) lower end is fixedly connected by connecting rod with turntable (7), under the turntable (7)
It holds and input shaft, the output axis connection of the input shaft and motor (18) of the turntable (7), the motor is fixedly installed at axial location
(18) it is fixedly mounted by motor mount and rack (8), turntable (7) lower end passes through plane bearing (17) and rack (8)
Cooperation installation, the internal engagement installation heating plate (27) of the pallet (23), the heating plate (27), solenoid valve (9), motor
(18), electric telescopic rod one (11), electric telescopic rod two (12), electric telescopic rod three (13) and electric telescopic rod four (14) is defeated
Enter end to be electrically connected with the output end of microcontroller (16), the microcontroller (16) is fixedly mounted with rack (8), the microcontroller (16)
Input terminal be electrically connected with the output end of external power supply.
2. a kind of extraction according to claim 1 stem cell extraction equipment small to cell contamination soon, it is characterised in that：Institute
State setting temperature sensor (28), the input of the output end and microcontroller (16) of the temperature sensor (8) inside retort (1)
End electrical connection.
3. a kind of extraction according to claim 1 stem cell extraction equipment small to cell contamination soon, it is characterised in that：Institute
It states and three pressing plates (21) is set outside retort (1), and three pressing plates (21) are uniformly arranged around the axis of retort (1).
4. a kind of extraction according to claim 3 stem cell extraction equipment small to cell contamination soon, it is characterised in that：Institute
It is L-shaped structure to state pressing plate (21), and the centre position of pressing plate (21) is connect by hinge with pallet (23), and under pressing plate (21)
End is connect by electric telescopic rod five (25) with pallet (23), input terminal and the microcontroller (16) of the electric telescopic rod five (25)
Output end electrical connection.
5. a kind of extraction according to claim 4 stem cell extraction equipment small to cell contamination soon, it is characterised in that：Institute
It states and installs foam-rubber cushion (22), the inner wall of the foam-rubber cushion (22) and pressing plate (21) between the upper end of pressing plate (21) and retort (1) additional
It is fixedly mounted.
6. a kind of extraction according to claim 1-5 stem cell extraction equipment small to cell contamination soon, it is characterised in that：
Installation connecting tube (19) between syringe one (2) and syringe two (3) and solenoid valve (9), the connecting tube (19) and syringe one (2),
It is cooperation really up to the mark between syringe two (3) and solenoid valve (9), and connecting tube (19) is flexible rubber hose.
7. a kind of extraction according to claim 1-6 stem cell extraction equipment small to cell contamination soon, it is characterised in that：
Pallet (23) is installed by connector and workbench (6) cooperation, and the connector is rubber block.
8. a kind of extracting stem cell extracting method small to cell contamination soon, it is characterised in that：Reaction enzymes are packed into syringe 1
Adipose tissue is packed into inside syringe 23 by portion, is then correctly assembled syringe 1 and syringe 23 with retort 1, by retort
1 is placed on 23 surface of pallet, and microcontroller 16 controls electric telescopic rod 5 25 and acts, and pressing plate 21 is made to consolidate retort 1 and pallet 23
Fixed, then microcontroller 16 controls heating plate 27 and 28 collective effect of temperature sensor, so that 1 inside of retort is kept constant temperature, and same
When microcontroller 16 control electric telescopic rod 3 13 and electric telescopic rod 4 14 and act, drive syringe 23 to work, adipose tissue made to exist
1 inside persistent movement of syringe 23 and retort, while electric telescopic rod 1 and electric telescopic rod 2 12 act, and control syringe
The automatic addition of reaction enzymes is realized in one 2 movements, while supersonic generator 24 works, and so that adipose tissue itself is generated vibrations, adds
Fast adipose tissue decomposes and emulsification, and after the completion, solenoid valve 9 is closed, and motor 18 works, and is driven workbench 6 to rotate, is made adipose tissue
It is centrifuged inside retort 1, by stem cell since its hetero-organization detaches.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810455773.7A CN108485916B (en) | 2018-05-14 | 2018-05-14 | Stem cell extraction equipment with rapid extraction and small cell pollution |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810455773.7A CN108485916B (en) | 2018-05-14 | 2018-05-14 | Stem cell extraction equipment with rapid extraction and small cell pollution |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108485916A true CN108485916A (en) | 2018-09-04 |
| CN108485916B CN108485916B (en) | 2021-08-17 |
Family
ID=63353928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810455773.7A Expired - Fee Related CN108485916B (en) | 2018-05-14 | 2018-05-14 | Stem cell extraction equipment with rapid extraction and small cell pollution |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108485916B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110144338A (en) * | 2019-05-29 | 2019-08-20 | 通用生物系统（安徽）有限公司 | It is a kind of using RNA as the RNA polymerase preparation method of template and the application of the polymerase |
| CN113329809A (en) * | 2018-12-08 | 2021-08-31 | 魏民 | Apparatus for producing cell therapy products |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030215935A1 (en) * | 2002-05-14 | 2003-11-20 | Coon David James | Apparatus and method for isolating living cells from an encapsulated organ tissue sample |
| WO2015010585A1 (en) * | 2013-07-26 | 2015-01-29 | 中国人民解放军第三军医大学第一附属医院 | Injectable tissue engineered cartilage in vitro construction apparatus |
| CN104684493A (en) * | 2012-08-29 | 2015-06-03 | 优而美股份公司 | Apparatus for extracting and re-injecting adipose tissue |
| WO2015131087A8 (en) * | 2014-02-28 | 2015-11-19 | Cytovera Inc. | A system for tissue manipulation |
| CN204939466U (en) * | 2015-08-27 | 2016-01-06 | 苏州瑞思坦医疗技术有限公司 | SVF cell filtering device in fatty tissue |
| CN105586248A (en) * | 2016-01-25 | 2016-05-18 | 上海东富龙科技股份有限公司 | Special rapid extraction equipment for stem cells |
| CN107058084A (en) * | 2017-02-17 | 2017-08-18 | 广西医科大学 | SVF automatic cell separating devices and its method |
| CN107164224A (en) * | 2017-07-04 | 2017-09-15 | 卢云丽 | A kind of syringe available for cell extraction |
-
2018
- 2018-05-14 CN CN201810455773.7A patent/CN108485916B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030215935A1 (en) * | 2002-05-14 | 2003-11-20 | Coon David James | Apparatus and method for isolating living cells from an encapsulated organ tissue sample |
| CN104684493A (en) * | 2012-08-29 | 2015-06-03 | 优而美股份公司 | Apparatus for extracting and re-injecting adipose tissue |
| WO2015010585A1 (en) * | 2013-07-26 | 2015-01-29 | 中国人民解放军第三军医大学第一附属医院 | Injectable tissue engineered cartilage in vitro construction apparatus |
| WO2015131087A8 (en) * | 2014-02-28 | 2015-11-19 | Cytovera Inc. | A system for tissue manipulation |
| CN204939466U (en) * | 2015-08-27 | 2016-01-06 | 苏州瑞思坦医疗技术有限公司 | SVF cell filtering device in fatty tissue |
| CN105586248A (en) * | 2016-01-25 | 2016-05-18 | 上海东富龙科技股份有限公司 | Special rapid extraction equipment for stem cells |
| CN107058084A (en) * | 2017-02-17 | 2017-08-18 | 广西医科大学 | SVF automatic cell separating devices and its method |
| CN107164224A (en) * | 2017-07-04 | 2017-09-15 | 卢云丽 | A kind of syringe available for cell extraction |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113329809A (en) * | 2018-12-08 | 2021-08-31 | 魏民 | Apparatus for producing cell therapy products |
| CN113329809B (en) * | 2018-12-08 | 2023-01-31 | 魏民 | Apparatus for producing cell therapy products |
| CN110144338A (en) * | 2019-05-29 | 2019-08-20 | 通用生物系统（安徽）有限公司 | It is a kind of using RNA as the RNA polymerase preparation method of template and the application of the polymerase |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108485916B (en) | 2021-08-17 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN108485916A (en) | It is a kind of to extract stem cell extraction equipment small to cell contamination soon | |
| CN204769670U (en) | Washing device of immunodetection analytical instrument | |
| CN110358673A (en) | Cell separation system and method | |
| CN105879148A (en) | IoT (Internet of Things)-based medical intelligent robot | |
| CN106235907A (en) | The multifunctional vacuum PTC with wifi module heats wall-breaking machine | |
| CN112403023A (en) | Equipment and method for extracting effective components of traditional Chinese medicine | |
| CN105586248B (en) | Special stem cell rapid extraction equipment | |
| CN114561270B (en) | High pathogenic virus culture container system | |
| RU2015141950A (en) | SYSTEM FOR ISOLATING CELLS FROM A TISSUE SAMPLE | |
| CN103250569B (en) | Automatic intermittent liquid culture inoculation system for edible mushrooms | |
| CN205501277U (en) | Equipment is drawed fast to special stem cell | |
| CN211436181U (en) | Equipment for removing hydroxyl in methyl silicone oil | |
| CN216653464U (en) | Device for extracting isoflavone from fermented soybean and recovering residue under assistance of ultrasonic waves | |
| CN202951021U (en) | Breast pump with memorizing and learning functions | |
| CN2333397Y (en) | Chinese medicine extracting pot | |
| CN211303440U (en) | Online sterilization tubular centrifuge | |
| CN115216397A (en) | Full-automatic nucleic acid extraction amplification diagnosis all-in-one | |
| CN203702587U (en) | Vertical pump mechanical seal leakage sensor monitoring system | |
| CN209438058U (en) | A kind of sterile concentrator of medicine extraction intelligence | |
| CN214419083U (en) | Softening treatment equipment for manufacturing bamboo fibers | |
| CN219985550U (en) | Reusable elution apparatus | |
| CN218686503U (en) | Extraction tank with uniform distribution of hot steam | |
| CN219010387U (en) | Heat treatment equipment for speed reducer box | |
| CN110756339A (en) | Online sterilization tube centrifuge and optimization system and use steps | |
| CN219090475U (en) | Wearable breast pump with physiotherapy function |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Ren Siying Inventor after: Hu Yan Inventor after: Wang Li Inventor after: Wu Wenjiao Inventor before: Wu Wenjiao |
|
| CB03 | Change of inventor or designer information | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20210728 Address after: 410011 No. 139 Renmin Middle Road, Hunan, Changsha Applicant after: THE SECOND XIANGYA HOSPITAL OF CENTRAL SOUTH University Address before: 313000 no.80-2, meiskou village, Hanshan village, Shanlian Town, Nanxun District, Huzhou City, Zhejiang Province Applicant before: HUZHOU HANHUI IMPORT AND EXPORT Co.,Ltd. |
|
| TA01 | Transfer of patent application right | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210817 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |