CN105586248B - Special stem cell rapid extraction equipment - Google Patents
Special stem cell rapid extraction equipment Download PDFInfo
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- CN105586248B CN105586248B CN201610048631.XA CN201610048631A CN105586248B CN 105586248 B CN105586248 B CN 105586248B CN 201610048631 A CN201610048631 A CN 201610048631A CN 105586248 B CN105586248 B CN 105586248B
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- Prior art keywords
- syringe
- stem cell
- extraction equipment
- screw socket
- rapid extraction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
Abstract
The invention discloses a kind of special stem cell rapid extraction equipment, it is mainly used in the clean environment in operating room, propulsion plant moves back and forth the adipose tissue in syringe in two syringes, after the tissue finally obtained fully enters collection syringe, obtained stem cell is injected into human body using syringe is collected.A small confined space is formed between three syringes, the time that adipose tissue is contacted with the external world is few, and the contaminated risk of stem cell extracted is greatly reduced.Of the invention to be compared with common stem cell extraction equipment, without using digestive ferment, the active karyocyte yield of acquisition is high, improves therapeutic effect.Small volume and less weight of the present invention, cost are low, and its cost is well below existing miscellaneous equipment;And extraction time is short, speed is fast, and whole work can be completed in 10 minutes, is saved on the basis of existing equipment extraction time for 3/4 or so time, safe and reliable.
Description
Technical field
The present invention relates to a kind of special stem cell rapid extraction equipment, belong to technical field of medical equipment.
Background technology
Autologous fat transplantation is a kind of generally acknowledged therapeutic modality for having extensive clinical indication, including Soft-tissue operation increases
Greatly, radiotherapy and post-traumatic tissue regions, and a variety of cosmetic applications are improved.From adipose tissue stromal vascular cell mass
The related progenitor cells of autologous vein are extracted, are added it in fat transfer tissue, to improve the effect and reliability of fat transfer,
This is a new technology, is also the theme of many clinical researches.Involve the safety of the fat transfer of data logging cellular modalities
Property and validity, this enhance separate SVF cells in operating room from liposuction aspirates to the demand of apparatus.
Available a variety of manual, semi-automatic, automatic SVF piece-rate systems, are placed on the crucial position of operating room in the market
Put, for extracting SVF to increase fat transfer effect.In the market adipose tissue SVF cell separation systems have the Multi of South Korea
The equipment such as Station, CHA-Station and Lipo-kit with MaxStem, the CRS Syetem in the U.S., substantially by with
Under several parts composition:Centrifuge, vibrations constant temperature machine, the fatty machine of suction, sterile bag, experiment tube etc..The conventional method of extraction be by
The fat of extraction is injected into experiment tube, is then placed in centrifuge and centrifuges, and adds after clostridiopetidase A, then be put into vibrations constant temperature machine
It is upper to be mixed, neutralize, wash, a series of processes such as cell filtration.
The equipment that above-mentioned adipose tissue SVF cell separation processes are related to is relatively more, and the time of consumption is longer, wherein most fast
CHA-Station equipment extract the time be also required to complete for 40 minutes, and during extraction cell by the general of risk
Rate is than larger, and the cost of every equipment is higher.
The content of the invention
The technical problem to be solved in the present invention is:There is provided a kind of small volume and less weight, cost is low, extraction time is short, safely can
The special stem cell rapid extraction equipment leaned on, solves the equipment that in the market adipose tissue SVF cell separation systems are related to and compares
Many, the time of consumption is longer, and cell is larger by the likelihood ratio of risk during extraction, and every equipment manufacturing cost is higher asks
Topic.
In order to solve the above-mentioned technical problem, the technical scheme is that being set there is provided a kind of special stem cell rapid extraction
Standby, it is characterised in that including framework, the bottom of framework is provided with propulsion plant, the telescopic end of propulsion plant and the bottom of connecting plate
Connection, connecting plate is enclosed on the axis of guide, and the two ends of the axis of guide are connected with the guide shaft support being fixed on framework, connecting plate
Top is connected with connector, and connector two ends connect one end of the first catch bar, one end of the second catch bar respectively, and first promotes
The other end of bar, the other end of the second catch bar are connected with two propulsion assemblies respectively, and first is provided between two propulsion assemblies
Syringe and the second syringe, the first syringe and the second syringe are on the framework, the dispensing end of the first syringe and the
The openend connection of one screw socket two-port valve, another openend of the first screw socket two-port valve connects with an openend of screw socket triple valve
Connect, another openend of screw socket triple valve is connected with the dispensing end of the second syringe, the middle opening end of screw socket triple valve and the
The openend connection of two screw socket two-port valves, another openend of the second screw socket two-port valve is injected by cell filter with collecting
The dispensing end connection of device.
Preferably, the two ends of described propulsion plant are connected with the bearing that is fixed on framework, the first fixed plate respectively.
Preferably, connected between described connecting plate and the axis of guide by linear bearing.
Preferably, the left and right sides of described connecting plate is respectively fitted on two axis of guides, and propulsion plant is led located at two
To between axle.
Preferably, the first described syringe and the second syringe are on support base, and support base is fixed on framework.
Preferably, described propulsion assembly includes the second fixed plate, and the second fixed plate is connected by hinge with portable plate.
Preferably, the second described fixed plate and the structure of portable plate are right-angle structure.
Preferably, the second described fixed plate is provided with a top plate.
It is of the invention to be compared with common stem cell extraction equipment, without using digestive ferment, the active karyocyte yield of acquisition
Height, improves therapeutic effect.Small volume and less weight of the present invention, cost are low, and its cost is well below existing miscellaneous equipment;And when extracting
Between it is short, speed is fast, and whole work can be completed in 10 minutes, and 3/4 or so is saved on the basis of existing equipment extraction time
Time, safe and reliable, the fat stem cell extracted using the present invention is used for clinical or experiment.
The present invention is mainly used in the clean environment in operating room, and propulsion plant is by the adipose tissue in syringe two
Move back and forth, after the tissue finally obtained fully enters collection syringe, incited somebody to action using syringe is collected in individual syringe
To stem cell be injected into human body.A small confined space is formed between three syringes, adipose tissue is contacted with the external world
Time it is few, the contaminated risk of stem cell extracted is greatly reduced.
Brief description of the drawings
Fig. 1 is a kind of structural representation of special stem cell rapid extraction equipment;
Fig. 2 is Fig. 1 top view;
The structural representation of propulsion assembly when Fig. 3 is for minor diameter syringe;
The structural representation of propulsion assembly when Fig. 4 is for major diameter syringe;
Fig. 5 is schematic diagram when compact heap compresses major diameter syringe;
Fig. 6 is schematic diagram when compact heap compresses minor diameter syringe.
Embodiment
To become apparent the present invention, hereby with preferred embodiment, and accompanying drawing is coordinated to be described in detail below.
The present invention is a kind of special stem cell rapid extraction equipment, and as shown in Figure 1 and Figure 2, it includes propulsion plant 11, pushes away
Enter the two ends of device 11 to be attached with the bearing 12 that is fixed on framework 25, the first fixed plate 5 respectively, it is ensured that propulsion plant 11
It is fixed.The telescopic end of propulsion plant 11 is with the bottom of connecting plate 10 by pin connection, and propulsion plant 11 is led installed in two
To between axle 8, the left and right sides of connecting plate 10 is respectively fitted on two axis of guides 8, and connecting plate 10 is connected with linear bearing 9, is led
It is fixed on to the two ends of axle 8 in guide shaft support 7, guide shaft support 7 is fixed on framework 25, connecting plate 10 passes through linear bearing
9 move back and forth on the axis of guide 8.The top of connecting plate 10 is connected with connector 4, and the two ends of connector 4 connect the first promotion respectively
One end of bar 3, one end of the second catch bar 6, the other end of the first catch bar 3, the second catch bar 6 the other end respectively with two
Propulsion assembly 1 is connected.First syringe 14 and the second syringe 17, the first syringe 14 are installed between two propulsion assemblies 1
It is each attached to the second syringe 17 on support base 2, support base 2 is fixed on framework 25.The dispensing end of first syringe 14 with
The openend connection of first screw socket two-port valve 15, another openend of the first screw socket two-port valve 15 and the one of screw socket triple valve 16
Openend is connected, and another openend of screw socket triple valve 16 is connected with the dispensing end of the second syringe 17, screw socket triple valve 16
Middle opening end is connected with an openend of the second screw socket two-port valve 19, another openend of the second screw socket two-port valve 19 and cell
One end connection of filter 20, the other end of cell filter 20 is connected with collecting the dispensing end of syringe 21.
In actual use, the second syringe 17 and the first empty syringe 14 of adipose tissue are will be equipped with, and
First screw socket two-port valve 15, screw socket triple valve 16, the second screw socket two-port valve 19, cell filter 20, collection syringe 21 etc. are even
Together be placed on support base 2 after connecting, rotary screw 18 by compact heap 13 compress the first syringe 14, the second syringe 17 in
On support base 2.After treating that all install, the second screw socket two-port valve 19 is closed, the first screw socket two-port valve 15 is opened, promoted
Device 11 is started working.
The telescopic end of propulsion plant 11 stretches out, and drives connecting plate 10 to be moved on the axis of guide 8, and the second catch bar 6 drives and pushed away
Enter component 1 to move, propulsion assembly 1 promotes the push rod movement of the second syringe 17, the shell of the second syringe 17 is solid by neck
Be scheduled on support base 2, the adipose tissue in the second syringe 17 with propulsion assembly 1 injection from promotion to the second syringe 17
End movement.Then, the telescopic end of propulsion plant 11 is retracted, and drives connecting plate 10 to be moved on the axis of guide 8, the band of the first catch bar 3
Dynamic propulsion assembly 1 is moved, and propulsion assembly 1 promotes the push rod movement of the first syringe 14, and the shell of the first syringe 14 passes through card
Groove is fixed on support base 2, and the adipose tissue in the first syringe 14 is as the promotion of propulsion assembly 1 is to the first syringe 14
Dispensing end is moved.
Adipose tissue in second syringe 17 is moved back and forth between the first syringe 14 and the second syringe 17, is being moved
In dynamic process, adipose tissue is also self emulsifying process.After motion a period of time terminates, adipose tissue is still rested on
In second syringe 17, the first screw socket two-port valve 15 is closed, the second screw socket two-port valve 19 is opened, the work again of propulsion plant 11
Make, adipose tissue is promoted into collection syringe 21 by screw socket triple valve 16, the second screw socket two-port valve 19, cell filter 20
In.The second screw socket two-port valve 19 is finally closed, collection syringe 21 is removed, the adipose tissue collected in syringe 21 can be straight
Tap into row clinical injection or experiment is used.The stretching motion of whole process propulsion plant 11 is all automatically controlled.
Because the amount of demand is different, the extraction fat stem cell of efficient quick is, it is necessary to use the note of different size for convenience
Emitter.To adapt to the syringe of different model specification, propulsion assembly 1 is made adjustable, and as shown in Figure 3, Figure 4, propulsion assembly 1 is wrapped
The second fixed plate 24 is included, the second fixed plate 24 is connected by hinge 23 with portable plate 22.Second fixed plate 24 and portable plate 22
Structure is right-angle structure.A top plate 26 is fixed with second fixed plate 24.When connecting minor diameter syringe, portable plate 22
As shown in Figure 3;When connecting major diameter syringe, by 90 ° of 22 rotate counterclockwise of portable plate in Fig. 3, top plate 26 is withstood, is such as schemed
Shown in 4.Compact heap 13 also make it is universal, when connect major diameter syringe when, compact heap 13 is compressed as shown in Figure 5;When
When connecting minor diameter syringe, the compact heap 13 in Fig. 5 is installed in turn and compressed, as shown in Figure 6.
To avoid cross pollution, the more use of highly effective and safe equipment often completes a fat stem cell extraction process,
It need to will promote the first syringe 14, the second syringe 17, collect syringe 21, cell filter 20, screw socket triple valve 16, first
Screw socket two-port valve 15, the second screw socket two-port valve 19 all more the renew and part by sterilizing, these are medical disposable material,
But cheap, buying is easy.
Claims (8)
1. a kind of special stem cell rapid extraction equipment, it is characterised in that including framework (25), the bottom of framework (25), which is provided with, to push away
Enter device (11), the telescopic end of propulsion plant (11) is connected with the bottom of connecting plate (10), and connecting plate (10) is enclosed on the axis of guide (8)
On, the two ends of the axis of guide (8) are connected with the guide shaft support (7) being fixed on framework (25), the top of connecting plate (10) with
Connector (4) is connected, and connector (4) two ends connect one end of the first catch bar (3), one end of the second catch bar (6) respectively, the
The other end of one catch bar (3), the other end of the second catch bar (6) are connected with two propulsion assemblies (1) respectively, two propulsion groups
The first syringe (14) and the second syringe (17) are provided between part (1), the first syringe (14) and the second syringe (17) are
On framework (25), the dispensing end of the first syringe (14) is connected with an openend of the first screw socket two-port valve (15), and first
Another openend of screw socket two-port valve (15) is connected with an openend of screw socket triple valve (16), screw socket triple valve (16) it is another
Openend is connected with the dispensing end of the second syringe (17), the middle opening end of screw socket triple valve (16) and the second screw socket two-port valve
(19) openend connection, another openend of the second screw socket two-port valve (19) is injected by cell filter (20) with collecting
The dispensing end connection of device (21).
2. a kind of special stem cell rapid extraction equipment as claimed in claim 1, it is characterised in that described propulsion plant
(11) two ends are connected with the bearing (12) that is fixed on framework (25), the first fixed plate (5) respectively.
3. a kind of special stem cell rapid extraction equipment as claimed in claim 1, it is characterised in that described connecting plate (10)
It is connected between the axis of guide (8) by linear bearing (9).
4. a kind of special stem cell rapid extraction equipment as claimed in claim 1, it is characterised in that described connecting plate (10)
The left and right sides be respectively fitted on two axis of guides (8), propulsion plant (11) is between two axis of guides (8).
5. a kind of special stem cell rapid extraction equipment as claimed in claim 1, it is characterised in that the first described syringe
(14) it is located at the second syringe (17) on support base (2), support base (2) is fixed on framework (25).
6. a kind of special stem cell rapid extraction equipment as claimed in claim 1, it is characterised in that described propulsion assembly
(1) the second fixed plate (24) is included, the second fixed plate (24) is connected by hinge (23) with portable plate (22).
7. a kind of special stem cell rapid extraction equipment as claimed in claim 6, it is characterised in that the second described fixed plate
(24) structure with portable plate (22) is right-angle structure.
8. a kind of special stem cell rapid extraction equipment as claimed in claim 6, it is characterised in that the second described fixed plate
(24) provided with a top plate (26).
Priority Applications (1)
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CN201610048631.XA CN105586248B (en) | 2016-01-25 | 2016-01-25 | Special stem cell rapid extraction equipment |
Applications Claiming Priority (1)
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CN201610048631.XA CN105586248B (en) | 2016-01-25 | 2016-01-25 | Special stem cell rapid extraction equipment |
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CN105586248A CN105586248A (en) | 2016-05-18 |
CN105586248B true CN105586248B (en) | 2017-10-24 |
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Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
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CN106754299B (en) * | 2016-12-28 | 2023-07-25 | 昆明医科大学 | Tumor tissue inoculating needle |
CN108102893A (en) * | 2017-12-29 | 2018-06-01 | 天津普瑞赛尔生物科技有限公司 | A kind of processing equipment of stem cell extraction |
CN108485916B (en) * | 2018-05-14 | 2021-08-17 | 中南大学湘雅二医院 | Stem cell extraction equipment with rapid extraction and small cell pollution |
CN109731498A (en) * | 2019-02-25 | 2019-05-10 | 乔爱军 | A kind of nanometer of fat or fatty glue boost motor |
CN116240094B (en) * | 2023-05-08 | 2023-08-04 | 北京纳通医疗科技控股有限公司 | Separation device and SVF preparation method |
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WO2005087292A1 (en) * | 2004-03-11 | 2005-09-22 | Harvest Technologies Corporation | Method for obtaining an intermediate fraction in the centrifugal separation of blood |
WO2007016344A1 (en) * | 2005-07-28 | 2007-02-08 | The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services | Detecting and characterizing macromolecular interactions in a solution with a simultaneous measurement of light scattering and concentration |
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Address after: 201109 Building 4, No. 1509, Duhui Road, Minhang District, Shanghai Patentee after: Dongfulong Technology Group Co.,Ltd. Address before: 201109 Building 4, No. 1509, Duhui Road, Minhang District, Shanghai Patentee before: SHANGHAI TOFFLON SCIENCE AND TECHNOLOGY Co.,Ltd. |