CN106754299B - Tumor tissue inoculating needle - Google Patents
Tumor tissue inoculating needle Download PDFInfo
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- CN106754299B CN106754299B CN201611231529.XA CN201611231529A CN106754299B CN 106754299 B CN106754299 B CN 106754299B CN 201611231529 A CN201611231529 A CN 201611231529A CN 106754299 B CN106754299 B CN 106754299B
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- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/07—Dosage or metering devices therefore
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Abstract
The invention provides a tumor tissue inoculating needle which comprises a main needle tube, a needle head connected to the main needle tube, a main plunger slidingly arranged in the main needle tube, a push needle and an inoculating auxiliary needle. The push needle is slidingly arranged in the main push plug and the needle head; at least one inoculating auxiliary needle is arranged on the main needle tube, and the inoculating auxiliary needle comprises an auxiliary needle tube communicated with the main needle tube and an auxiliary plunger slidingly arranged in the auxiliary needle tube. The tumor tissue inoculating needle is characterized in that the main needle tube is in the form of a sleeve needle, the inoculating auxiliary needle is additionally arranged on the main needle tube, and tumor tissue is stored in the auxiliary needle tube, so that on one hand, the tumor tissue can be conveniently added into the auxiliary needle tube, on the other hand, a certain amount of tumor tissue can be stored in the inoculating auxiliary needle, multiple times of inoculation can be performed, repeated and troublesome loading and unloading of the tumor tissue are not needed, and the tumor tissue inoculating needle is capable of continuously inoculating, convenient in tumor tissue loading and unloading, time-saving and labor-saving and high in inoculating efficiency.
Description
Technical Field
The invention relates to the field of syringes, in particular to a tumor tissue inoculating needle.
Background
In the field of medicine and pharmacology, in research, development, screening and evaluation of anti-tumor medicaments, establishment of a qualified animal tumor transplantation model is important for further research, and a plurality of methods are established for animal tumor transplantation model, wherein the small-block inoculation method has high tumor survival rate after inoculation, can enable the tumor growth volume to be larger, and has wide application.
The small-block inoculation method is to take out tumor tissue from the body of the animal (most mice) which has been kept, cut the tumor tissue into small blocks (about 5X 5 mm), put the small blocks into the inoculation site (usually the armpit of the animal), there are two methods for placing tumor tissue into the inoculation site of animals, one is to cut a small opening in the armpit of the recipient animal, and to clamp the tumor tissue small pieces with forceps and send them under the armpit endothelium. Another method is to insert a tumor tissue mass into the trocar, penetrate the trocar from the animal side abdomen, pass subcutaneously under the armpit, and then push the tumor tissue mass out of the trocar for inoculation.
The recipient animal is inoculated by the traditional tumor tissue block inoculation method in a place where the recipient animal is not satisfactory, and the tumor tissue block is clamped by forceps, so that on one hand, the armpit incision of the animal needs to be opened more greatly, the armpit incision of the animal can be enlarged by using the forceps, the tumor tissue block is easy to leak and difficult to grow, and the tumor tissue block grows slowly after being inoculated. When the trocar is used, the trocar cannot quantify the tissue volume, the loading of tumor tissue blocks into the trocar is troublesome and time-consuming, and each loaded tumor tissue block can only be administered to one animal and cannot be continuously inoculated, so that the time-consuming, laborious and inconsistent inoculation amount of the tumor tissue of each animal are defects in the inoculation process.
Disclosure of Invention
In view of the above, the present invention aims to overcome the defects in the prior art, and provides a tumor tissue inoculating needle which can be continuously inoculated, is convenient for loading and unloading tumor tissues, saves time and labor, and has high inoculating efficiency.
In order to solve the problems, the invention provides the following solutions:
the tumor tissue inoculating needle comprises a main needle tube, a needle head connected to the main needle tube and a main plunger slidingly arranged in the main needle tube;
the injection needle also comprises a push needle and an inoculation auxiliary needle, wherein the push needle is slidingly arranged in the main push plug and the needle head;
at least one inoculating auxiliary needle is arranged on the main needle tube, and comprises an auxiliary needle tube communicated with the main needle tube and an auxiliary plunger slidingly arranged in the auxiliary needle tube.
In an exemplary embodiment, the length of the main plunger is not less than the length of the main needle cannula;
the length of the push pin is greater than the sum of the lengths of the main push plug and the needle.
In an exemplary embodiment, the push pin is provided with a limiting part;
when the limiting part is abutted with the main pushing plug, the extending length of the pushing needle from the main pushing plug is larger than the length of the needle head.
In an exemplary embodiment, the main plunger is a hollow tube, the hollow bore cooperating with the push pin;
the limiting part is a protrusion arranged near the end part of the push pin, and the protrusion limits the push pin to slide into the main push plug.
In an exemplary embodiment, each of the secondary syringes is marked with a volume scale.
In an exemplary embodiment, each of the secondary syringes is detachable from the primary syringe.
In an exemplary embodiment, the secondary needle cannula is threadably connected to the primary needle cannula.
In an exemplary embodiment, the secondary needle cannula is connected to the primary needle cannula by a snap fit connection.
In an exemplary embodiment, the interior bore of the needle cooperates with the push pin.
In an exemplary embodiment, the needle is detachable from the main needle cannula.
The tumor tissue inoculating needle is characterized in that the main needle tube is in the form of a sleeve needle, the inoculating auxiliary needle is additionally arranged on the main needle tube, and tumor tissue is stored in the auxiliary needle tube, so that on one hand, the tumor tissue can be conveniently added into the auxiliary needle tube, on the other hand, a certain amount of tumor tissue can be stored in the inoculating auxiliary needle, multiple times of inoculation can be performed, repeated and troublesome loading and unloading of the tumor tissue are not needed, and the tumor tissue inoculating needle is capable of continuously inoculating, convenient in tumor tissue loading and unloading, time-saving and labor-saving and high in inoculating efficiency.
The auxiliary needle tube of the auxiliary inoculation needle is marked with volume scales, and the tumor tissue injected in the auxiliary inoculation needle can be pushed out from the needle head through the structure of the sleeve needle, so that the amount of the tumor tissue injected in the auxiliary inoculation needle is the inoculation amount, and the tumor tissue inoculation needle with good inoculation effect can be quantitatively injected.
The detachable connection between the auxiliary inoculating needle and the main needle tube ensures that the tumor tissue is more convenient to assemble and disassemble and has higher assembling and disassembling efficiency.
In order to make the above objects, features and advantages of the present invention more comprehensible, preferred embodiments accompanied with figures are described in detail below.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are needed in the embodiments will be briefly described below, it being understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and other related drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic view showing an exploded structure of a tumor tissue inoculating needle according to a first embodiment of the present invention;
FIG. 2 is a schematic view showing the overall structure of a tumor tissue inoculating needle according to a first embodiment of the present invention;
FIG. 3 is a schematic view showing the overall structure of a tumor tissue inoculating needle according to a second embodiment of the present invention;
fig. 4 is a schematic view showing the overall structure of a tumor tissue inoculating needle according to a third embodiment of the present invention.
Description of main reference numerals:
1-tumor tissue inoculating needle; 11-a main needle tube; 111-interface; 12-needle; 13-a main plunger; 14-pushing needles; 141-a limiting part; 15-inoculating a secondary needle; 151-auxiliary needle tube; 152-secondary plunger.
Detailed Description
In order to facilitate an understanding of the present invention, a tumor tissue inoculating needle will be described more fully below with reference to the accompanying drawings. A preferred embodiment of a tumor tissue inoculating needle is shown in the drawings. However, the tumor tissue inoculating needle may be implemented in many different forms and is not limited to the embodiments described herein. Rather, the purpose of these embodiments is to provide a more thorough and complete disclosure of inoculating needles to tumor tissue.
It will be understood that when an element is referred to as being "fixed to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. In contrast, when an element is referred to as being "directly on" another element, there are no intervening elements present. The terms "vertical," "horizontal," "left," "right," and the like are used herein for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used herein in the description of tumor tissue inoculating needles is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. The term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.
The following describes the embodiments of the present invention in detail with reference to the drawings.
Example 1
Referring to fig. 1 and 2, a tumor tissue inoculating needle 1 is a syringe, and is mainly used for inoculating tumor tissue to treat diseases. The tumor tissue inoculating needle 1 is particularly suitable for small-dose inoculation. The tumor tissue inoculating needle 1 of the present embodiment exemplifies inoculating a tumor tissue to a mouse.
The tumor tissue inoculating needle 1 comprises a main needle tube 11, a needle head 12, a main plunger 13, a push needle 14 and an inoculating auxiliary needle 15. Wherein, the end part of the main needle tube 11 is connected with a needle head 12. A main plunger 13 is provided inside the main needle tube 11, and the main plunger 13 slides inside the main needle tube 11. The main plunger 13 is internally provided with a push pin 14, and the push pin 14 slides inside the main plunger 13. The inoculating sub-needle 15 is connected to the main needle tube 11, and the inoculating sub-needle 15 includes a sub-needle tube 151 and a sub-plunger 152, and the sub-plunger 152 is provided in the sub-needle tube 151 and slides in the sub-needle tube 151. The sub needle tube 151 communicates with the main needle tube 11.
The main needle tube 11 is the main body of the tumor tissue inoculating needle 1, and all components take the main needle tube 11 as a main carrier. The main plunger 13 pushes the injection in the main needle tube 11 into the needle head 12, the needle head 12 penetrates from the inoculation part, namely the armpit of the mouse, and the pushing needle 14 is pushed to inject the small injection in the needle head 12 into the armpit of the mouse. The auxiliary needle tube 151 is used for storing injection, namely tumor tissue, and the auxiliary needle 15 can be used for injecting small dose and simultaneously can be filled into the tumor tissue at one time, so that the injection is carried out on a plurality of mice without repeatedly adding the tumor tissue, the use is more convenient, the time and the labor are saved, the purity of the tumor tissue is higher, and the pollution caused by repeatedly adding the tumor tissue is avoided.
The number of the sub-inoculating needles 15 connected to the main needle tube 11 is at least one. In this embodiment, two inoculating sub-needles 15 are connected to the main needle tube 11. The advantage of having at least one inoculating sub-needle 15 attached is that a larger tumor tissue inoculation capacity can be achieved with multiple inoculating sub-needles 15 and more mice can be inoculated at a time. The two sub-needles 15 of the present embodiment meet the capacity requirement of continuous inoculation of a plurality of mice on the one hand. On the other hand, from the point of view of a reasonably optimized layout, two inoculating sub-needles 15 are used without affecting the user's grip on the main needle tube 11.
The main needle tube 11 is cylindrical, and the material of the main needle tube can be selected from nontoxic and harmless plastics, stainless steel or glass and the like. In this embodiment, the main needle tube 11 is a stainless steel metal tube, and stainless steel has good corrosion resistance, excellent chemical properties, excellent mechanical properties and durability, and the main needle tube 11 can be sterilized and reused after use, thereby reducing the cost and reducing the environmental pollution. The plastic main needle tube 11 is a disposable needle tube, and is discarded after use, so that the disposable main needle tube 11 is safer to use, and injection cross infection caused by unclean disinfection and cleaning does not exist.
The end part of the main needle tube 11 is connected with a needle head 12, the needle head 12 is the needle head 12 of a common injector, the end part of the needle head 12 is a hollow metal needle, the end part of the needle is sharp and is used for puncturing the armpit of a mouse, and the hollow structure is used for exhausting air on one hand and discharging liquid on the other hand. In this embodiment, the needle 12 is a stainless steel needle 12, which can be reused. The needle 12 is detachable from the main needle tube 11, for example, by a snap connection.
Because the needle 12 is relatively sharp and exposed outside for a long time, the damage to the needle tip is easy to cause, and the puncture effect of the needle 12 is affected, the needle 12 should be replaced in time when the needle 12 is damaged, and the detachable connection mode is adopted, so that the replacement of the needle 12 is more convenient. Meanwhile, the needle head 12 and the main needle tube 11 can be repeatedly used, and after the needle head is used, the needle head 12 and the main needle tube 11 which are detachable are respectively cleaned at the cleaning time, so that the cleaning is more thorough and comprehensive.
The main plunger 13 is a cylindrical hollow cylinder arranged inside the main needle tube 11, and the end part of the main plunger 13 is sleeved with a rubber sleeve. The main plunger 13 sleeved with the rubber sleeve is in interference fit with the inner wall of the main needle tube 11, namely, the rubber sleeve is extruded by the inner wall of the main needle tube 11 to generate deformation, and an elastic restoring force is generated along with the deformation, so that the main plunger 13 and the main needle tube 11 are sealed, and tumor tissues in the main needle tube 11 are extruded through the sliding of the main plunger 13 in the main needle tube 11.
The length of the main plunger 13 is not smaller than the length of the main needle tube 11, namely, when the rubber sleeve on the main plunger 13 is abutted against the bottom of the main needle tube 11, part of the main plunger 13 is exposed from the main needle tube 11. The advantage of this arrangement is that the main plunger 13, which is longer than the main needle tube 11, facilitates the pulling out after pushing in the main needle tube 11.
A push needle 14 is inserted into the hollow main push plug 13, and the push needle 14 is a solid round rod, and the size of the push needle is matched with that of the needle 12. The hollow bore of the main plunger 13 matches the diameter of the push needle 14. The push needle 14 slides in the main plunger 13, pushing through the main plunger 13 into the needle 12, thus pressing out the tumor tissue in the needle 12.
The length of the push pin 14 is longer than the sum of the lengths of the main push plug 13 and the needle 12. To ensure that the push pin 14 can pass through the needle 12 or at least be flush with the end face of the needle 12 to expel all of the tumor tissue from the needle 12. Because the inoculation amount of the tumor tissue of the mouse is less, the tumor tissue in the needle head 12 is completely discharged, so that the inoculation amount of the tumor tissue inoculation needle 1 is controlled more accurately, and a better inoculation effect is achieved.
In this embodiment, the end of the push pin 14 is provided with a push handle, by which the push pin 14 is pushed. Because of the small diameter and thinness of the push pin 14, it is inconvenient to operate when pushing and pulling the push pin 14. The pushing handle structure is additionally arranged at the end part of the pushing needle 14, so that the stress area of the pushing needle 14 is increased, and the pushing needle 14 is forced by the pushing handle, so that more labor is saved.
The push pin 14 close to the push handle is provided with a limiting part 141, the limiting part 141 is a bulge on the push pin 14, and when the limiting part 141 is abutted against the end part of the main push plug 13, the sliding of the push pin 14 in the main push plug 13 is limited. Specifically, when the stopper 141 abuts against the end surface of the main plunger 13, the push pin 14 protrudes in the main plunger 13 by a length longer than the length of the needle 12. Therefore, when the main plunger 13 is abutted with the main needle tube 11, the push needle 14 is pushed to the limit part 141 to be abutted with the main plunger 13, and the push needle 14 can completely penetrate through the needle head 12, so that no tumor tissue residue exists in the needle head 12, and the tumor tissue is completely discharged.
Specifically, in this embodiment, the length of the main needle tube 11 is 6cm, the length of the needle head 12 is 5cm, the length of the main plunger 13 is 6cm, and the length of the push needle 14 is 13cm. The limit portion 141 is disposed at a distance of 1.6cm from the end of the push needle 14, and it is known that when the limit portion 141 abuts against the main push plug 13, the push needle 14 extends from the needle 12 by a length of 13-6-5-1.6=0.4 cm, i.e. the push needle 14 can completely squeeze out the tumor tissue in the needle 12.
It will be appreciated that in other embodiments, the main needle cannula 11, needle 12, main plunger 13 may be of other sizes, with different sizes being selected for different injection requirements.
The main needle tube 11 is provided with the same number of ports 111 as the sub-inoculating needles 15, and the ports 111 are used for mounting the sub-inoculating needles 15.
The sub-inoculation needle 15 is actually a structure for storing and injecting tumor tissue into the main needle tube 11. The sub needle tube 151 is cylindrical, and the sub needle tube 151 is used for storing tumor tissue, one end of the sub needle tube 151 is communicated with the main needle tube 11, and the other end of the sub needle tube is inserted with a sub plunger 152. The end of the auxiliary plunger 152 is sleeved with a rubber sleeve, the auxiliary plunger 152 sleeved with the rubber sleeve is in interference fit with the inner wall of the auxiliary needle tube 151, namely, the rubber sleeve is extruded by the inner wall of the auxiliary needle tube 151 to generate deformation, and an elastic restoring force is generated along with the deformation, so that the auxiliary plunger 152 and the auxiliary needle tube 151 are sealed, and tumor tissues in the auxiliary needle tube 151 are extruded into the main needle tube 11 through sliding of the auxiliary plunger 152 in the auxiliary needle tube 151.
In this embodiment, two sub-syringes 151 are connected side by side to the main syringe 11, and the axis of the sub-syringe 151 is perpendicular to the axis of the main syringe 11. The advantage of this arrangement is that the appearance is neat and attractive. And the auxiliary pushing plug 152 is not extruded, and the tumor tissue therein does not flow by itself due to gravity, so that the injection amount of the tumor tissue of the main needle tube 11 by the inoculating auxiliary needle 15 is controlled more accurately.
The subsidiary needle tube 151 is marked with volume scales, and each scale is 0.1ml, which is suitable for inoculation of micro tumor tissues. The auxiliary needle tube 151 marked with volume scales enables the auxiliary needle 15 to be inoculated, and the injection amount of the tumor tissue of the main needle tube 11 is visual and controllable, so that the tumor tissue inoculating needle 1 becomes a quantitative and continuous inoculating needle.
The auxiliary needle tube 151 and the main needle tube 11 are detachably connected, in this embodiment, the auxiliary needle tube 151 and the main needle tube 11 are connected through threads, an internal thread is arranged on the interface 111 on the main needle tube 11, and an external thread matched with the internal thread is arranged on the auxiliary needle tube 151. The detachable connection of the main needle tube 11 and the sub needle tube 151 is achieved by screwing the sub needle tube 151 onto the interface 111. The threaded connection mode is adopted, the structure is simple, the assembly and the disassembly are convenient, the connection is reliable, and meanwhile, the threaded connection has excellent sealing performance.
In order to further ensure the tightness between the main needle tube 11 and the auxiliary needle tube 151, a sealing ring is further arranged at the interface 111, when the auxiliary needle tube 151 is screwed into the interface 111, the auxiliary needle tube 151 is tightly pressed, the sealing ring is elastically deformed, and an elastic restoring force is generated along with the elastic restoring force, and gaps between the main needle tube 11 and the auxiliary needle tube 151 are filled and tightly pressed, so that the sealing effect is achieved. It will be appreciated that the seal between the primary and secondary syringes 11, 151 is an end face seal.
In this embodiment, the inoculating auxiliary needle 15 is made of stainless steel, and can be repeatedly used after cleaning and sterilizing. The inoculating auxiliary needle 15 is detached from the main needle tube 11 and is filled with tumor tissue, the whole end face of the auxiliary needle tube 151 is an opening, and the tumor tissue is conveniently filled. The tumor tissue is quantitatively injected into the main needle tube 11 through the scale mark and the sub-plunger 152.
Specifically, the method for inoculating tumor tissue into mice comprises the following steps:
filling tumor tissue small blocks into a secondary needle tube 151 of the inoculation secondary needle 15; the subsidiary needle tube 151 is screwed to the main needle tube 11; pushing out 0.1-0.2ml tumor tissue pieces by the secondary plunger 152; pushing the main plunger 13 to the bottommost end of the main needle tube 11; the needle 12 is penetrated subcutaneously from the lateral abdomen of the mouse and passed to the armpit; pushing the push pin 14 to the bottom. Through the steps, the inoculation of the tumor tissue of one mouse is completed, and when the next mouse is inoculated, only 3-6 steps are needed to be repeated, the tumor tissue is not needed to be taken again, and a plurality of mice can be continuously inoculated.
Example 2
As shown in fig. 3, this embodiment is different from embodiment 1 in that in this embodiment, a main needle tube 11 is connected with an inoculating sub needle 15. One inoculating auxiliary needle 15 is provided with a large amount of holding space for the main needle tube 11 on the premise of meeting the requirement of continuous inoculation, so that the main needle tube 11 is more comfortable to hold.
The difference between this embodiment and embodiment 1 is that in this embodiment, the main needle tube 11 is connected with the auxiliary needle tube 151 through a clamping connection, specifically, a protrusion is provided on the interface 111, an annular groove is provided on the auxiliary needle tube 151, and the auxiliary needle tube 151 is clamped on the main needle tube 11 through the cooperation of the protrusion and the annular groove. The connection structure is simple by the clamping connection of the main needle tube 11 and the auxiliary needle tube 151, and the connection structure can be realized by plugging and unplugging when the device is assembled and disassembled. The holders of the main needle tube 11 and the auxiliary needle tube 151 are also provided with sealing rings, which are sleeved on the cylindrical surface of the auxiliary needle tube 151 to form cylindrical surface sealing with the interface 111 in the embodiment.
Example 3
As shown in fig. 4, this embodiment is different from embodiment 1 in that three sub inoculating needles are connected to the main needle tube 11 in this embodiment. Three subsidiary needle tubes 151 are distributed side by side on the main needle tube 11, fingers can be put on the subsidiary needle tube 151 when the main needle tube 11 is held, the holding comfort is not affected, meanwhile, the total capacity of the subsidiary needle tube 151 is larger, and more mice can be inoculated simultaneously.
The present embodiment is different from embodiment 1 in that in this embodiment, the sub-needle tube 151 is connected to the main needle tube 11 in a non-detachable manner, and the main needle tube 11 and the sub-needle tube 151 are integrally formed, so that the sealing between the main needle tube 11 and the sub-needle tube 151 is good. When the tumor tissue is to be loaded, the sub-plunger 152 is pulled out from the sub-needle tube 151, and the tumor tissue is added to the sub-needle tube 151 through the opening.
The tumor tissue inoculating needle has the following beneficial effects:
1. the tumor tissue inoculating needle is characterized in that the main needle tube is in the form of a sleeve needle, the inoculating auxiliary needle is additionally arranged on the main needle tube, and tumor tissue is stored in the auxiliary needle tube, so that on one hand, the tumor tissue can be conveniently added into the auxiliary needle tube, on the other hand, a certain amount of tumor tissue can be stored in the inoculating auxiliary needle, multiple times of inoculation can be performed, repeated and troublesome loading and unloading of the tumor tissue are not needed, and the tumor tissue inoculating needle is capable of continuously inoculating, convenient in tumor tissue loading and unloading, time-saving and labor-saving and high in inoculating efficiency.
2. The auxiliary needle tube of the auxiliary inoculation needle is marked with volume scales, and the tumor tissue injected in the auxiliary inoculation needle can be pushed out from the needle head through the structure of the sleeve needle, so that the amount of the tumor tissue injected in the auxiliary inoculation needle is the inoculation amount, and the tumor tissue inoculation needle with good inoculation effect can be quantitatively injected.
3. The detachable connection between the auxiliary inoculating needle and the main needle tube ensures that the tumor tissue is more convenient to assemble and disassemble and has higher assembling and disassembling efficiency.
Any particular values in all examples shown and described herein are to be construed as merely illustrative and not a limitation, and thus other examples of exemplary embodiments may have different values.
It should be noted that: like reference numerals and letters denote like items in the following figures, and thus once an item is defined in one figure, no further definition or explanation thereof is necessary in the following figures.
The above examples merely represent a few embodiments of the present invention, which are described in more detail and are not to be construed as limiting the scope of the present invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.
Claims (7)
1. The tumor tissue inoculating needle is used for inoculating tumor tissue and comprises a main needle tube, a needle head connected to the main needle tube and a main plunger slidingly arranged in the main needle tube, and is characterized in that,
the device also comprises a push needle and an inoculation auxiliary needle, wherein the push needle is slidingly arranged in the main push plug and the needle head, and a limiting part is arranged on the push needle; when the limiting part is abutted with the main pushing plug, the extending length of the pushing needle from the main pushing plug is larger than the length of the needle head;
the main plunger is a cylindrical hollow tube, and the hollow hole is matched with the push pin; the limiting part is a protrusion arranged near the end part of the push pin, and the protrusion limits the push pin to slide into the main push plug;
the length of the main pushing plug is not smaller than that of the main needle tube; the length of the push pin is greater than the sum of the lengths of the main push plug and the needle;
at least one inoculating auxiliary needle is arranged on the main needle tube, and comprises an auxiliary needle tube communicated with the main needle tube and an auxiliary plunger slidingly arranged in the auxiliary needle tube; the axis of the auxiliary needle tube is perpendicular to the axis of the main needle tube.
2. The tumor tissue inoculating needle of claim 1, wherein each of said secondary needle tubes is marked with a volume scale.
3. The tumor tissue inoculating needle of claim 1, wherein each of said secondary needle tubes is detachable from said primary needle tube.
4. A tumor tissue inoculating needle according to claim 3, wherein said secondary needle cannula is threadably connected to said primary needle cannula.
5. A tumor tissue inoculating needle according to claim 3, wherein said secondary needle cannula is connected to said primary needle cannula by a snap fit connection.
6. The tumor tissue inoculating needle of claim 1, wherein the inner bore of said needle head mates with said push pin.
7. The tumor tissue inoculating needle of claim 1, wherein said needle head is detachable from said main needle tube.
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| CN201611231529.XA CN106754299B (en) | 2016-12-28 | 2016-12-28 | Tumor tissue inoculating needle |
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| CN201611231529.XA CN106754299B (en) | 2016-12-28 | 2016-12-28 | Tumor tissue inoculating needle |
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| US11485948B2 (en) * | 2018-06-12 | 2022-11-01 | Sanko Tekstil Isletmeleri San. Ve Tic. A.S. | Process for providing a culture of microorganisms to an elongated element |
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| KR200473199Y1 (en) * | 2014-03-28 | 2014-06-18 | 김학범 | Medical complex anesthesia apparatus |
| CN204501099U (en) * | 2015-03-28 | 2015-07-29 | 黑龙江职业学院 | A kind of animal and veterinary gun-type syringe |
| CN204655780U (en) * | 2015-05-06 | 2015-09-23 | 青岛市中心医院 | A kind of Urology Surgery anesthetic machine |
| CN205198606U (en) * | 2015-12-11 | 2016-05-04 | 宫兆玉 | A needle is injected to novel bacterin for immunization campaign |
| CN205586113U (en) * | 2016-03-23 | 2016-09-21 | 内蒙古草原宏宝食品股份有限公司 | Veterinary injection gun |
| CN206476987U (en) * | 2016-12-28 | 2017-09-08 | 昆明医科大学 | Microbial inoculant pin |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105586248A (en) * | 2016-01-25 | 2016-05-18 | 上海东富龙科技股份有限公司 | Special rapid extraction equipment for stem cells |
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| CN106754299A (en) | 2017-05-31 |
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