CN110283719B - Autologous fat active cell filtering device for plastic cosmetology - Google Patents
Autologous fat active cell filtering device for plastic cosmetology Download PDFInfo
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- CN110283719B CN110283719B CN201910516003.3A CN201910516003A CN110283719B CN 110283719 B CN110283719 B CN 110283719B CN 201910516003 A CN201910516003 A CN 201910516003A CN 110283719 B CN110283719 B CN 110283719B
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- 238000001914 filtration Methods 0.000 title claims abstract description 68
- 239000004033 plastic Substances 0.000 title claims abstract description 7
- 230000029087 digestion Effects 0.000 claims abstract description 34
- 238000000926 separation method Methods 0.000 claims abstract description 28
- 239000011148 porous material Substances 0.000 claims abstract description 6
- 210000001519 tissue Anatomy 0.000 claims description 32
- 210000000577 adipose tissue Anatomy 0.000 claims description 20
- 239000007788 liquid Substances 0.000 claims description 13
- 238000005070 sampling Methods 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 3
- 235000017166 Bambusa arundinacea Nutrition 0.000 claims 4
- 235000017491 Bambusa tulda Nutrition 0.000 claims 4
- 241001330002 Bambuseae Species 0.000 claims 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 claims 4
- 239000011425 bamboo Substances 0.000 claims 4
- 239000004429 Calibre Substances 0.000 claims 1
- 239000002537 cosmetic Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 abstract description 54
- 239000000706 filtrate Substances 0.000 abstract description 21
- 239000002245 particle Substances 0.000 abstract description 13
- 210000003743 erythrocyte Anatomy 0.000 abstract description 7
- 239000012535 impurity Substances 0.000 abstract description 7
- 238000000746 purification Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 description 7
- 230000009471 action Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 102000029816 Collagenase Human genes 0.000 description 3
- 108060005980 Collagenase Proteins 0.000 description 3
- 229960002424 collagenase Drugs 0.000 description 3
- 238000002316 cosmetic surgery Methods 0.000 description 3
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- 238000004140 cleaning Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- 239000010410 layer Substances 0.000 description 2
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- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 230000004069 differentiation Effects 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
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- 239000008187 granular material Substances 0.000 description 1
- 238000000265 homogenisation Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000011859 microparticle Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 231100000241 scar Toxicity 0.000 description 1
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- 238000012216 screening Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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Abstract
The invention relates to the technical field of cell filtration, and discloses an autologous fat active cell filtering device for plastic cosmetology, which comprises a container, a cell sieve and a collector, wherein the cell sieve comprises a filter cylinder, the bottom of the filter cylinder is connected with a collecting funnel, the edge of the large caliber of the collecting funnel is hermetically connected with the edge of the bottom of the filter cylinder, and the end of the small caliber of the collecting funnel is communicated with the inner cavity of the collector; the filtering device also comprises a separating cylinder arranged between the container and the cell sieve; an exhaust pipe is installed at the top of the collector, one end of the exhaust pipe is communicated with the inner cavity of the collector, and the other end of the exhaust pipe is used for being connected with an exhaust pump. According to the invention, the fat tissue digestion mixed liquor is pre-separated by using the separation cylinder, so that fat tissues and other large-particle impurities which are not completely digested are removed, and the problem that the filtering speed is influenced by the blockage of the sieve pores of the cell sieve is avoided, thereby improving the filtering efficiency; and further filtering by using a cell sieve to filter mixed cells such as red blood cells and the like in the pre-separation filtrate, so that the subsequent centrifugal separation and purification of the fat active cells are facilitated.
Description
Technical Field
The invention relates to the technical field of cell filtration, in particular to an autologous fat active cell filtering device for plastic cosmetology.
Background
With the aging, the human tissues are aged and the number of cells is reduced, so that the skin has aging symptoms of looseness, loss of elasticity, increase of wrinkles, even local depressions and the like. This drives people to pursue various techniques of retaining youthful looks, either for internal or external use.
Adipose-derived cells are isolated from adipose tissue, and are cells that are not fully differentiated, yet immature, and have a multipotent differentiation potential. Because of the advantages of abundant sources, convenient material acquisition, abundant stem cell content in tissues and the like, the adipose-derived activated cells are considered to be one of ideal cell sources for tissue regeneration and repair. The adipose tissue of the fat-rich parts such as waist, abdomen, hip, legs and the like of the patient is extracted from the autologous adipose-active cells, and only viable and healthy fresh and alive cells are finally reserved after the procedures of cleaning, collagenase digestion, filtration and centrifugal purification, so that the autologous adipose-active cells can be used for removing wrinkles, repairing scars, filling the face and the like, and are self-sourced and have no rejection.
The existing autologous fat living cell filtering device adopts a cell sieve for filtering, and because fat tissues which are not digested and dispersed by collagenase still exist in fat tissue digestion mixed liquid, sieve pores are blocked in the filtering process, and the filtering speed and the filtering efficiency are poor.
Disclosure of Invention
Based on the problems, the invention provides the autologous fat active cell filtering device for plastic cosmetology, which can remove fat tissues and other large-particle impurities in the fat tissue digestion mixed liquor in advance and prevent the filtering speed from being influenced by the blockage of the sieve pores of the cell sieve, thereby improving the filtering efficiency; and further filtering the fat tissue digestion mixed liquor by using a cell sieve, and filtering mixed cells such as red blood cells in the pre-separation filtrate, so as to facilitate the subsequent centrifugal separation and purification of fat active cells.
In order to solve the technical problems, the invention provides the following technical scheme:
a filtering device of autologous fat active cells for plastic cosmetology comprises a container for containing a fat tissue digestion mixed solution, a cell sieve for filtering the fat tissue digestion mixed solution and a collector for collecting filtrate, wherein the collector is provided with a top cover capable of sealing the collector; the cell sieve comprises a filter cylinder with a cover at the upper part and a filter screen at the bottom, the bottom of the filter cylinder is connected with a flow collecting funnel, the edge of a large caliber of the flow collecting funnel is hermetically connected with the edge of the bottom of the filter cylinder, and the end of a small caliber of the flow collecting funnel is communicated with the inner cavity of the collector through a connecting pipeline; the filtering device also comprises a separating cylinder for pre-separating the fat tissue digestion mixed liquor; the separating cylinder is of a double-layer structure and comprises an inner cylinder and an outer cylinder, sealing covers are arranged at the bottoms and the tops of the inner cylinder and the outer cylinder, the wall of the outer cylinder is connected with a feeding pipe, one end of the feeding pipe extends into the container, and the other end of the feeding pipe is communicated with an inner cavity of the outer cylinder of the filter cylinder; the wall of the inner barrel is provided with a plurality of filtering holes, the inner barrel is connected with a discharge pipe communicated with the inner cavity of the inner barrel, and the discharge end of the discharge pipe is communicated with the inner cavity of the cell sieve; an exhaust pipe is installed at the top of the collector, one end of the exhaust pipe is communicated with the inner cavity of the collector, and the other end of the exhaust pipe is used for being connected with an exhaust pump.
Further, the inner tube is a cyclone tube, each filtering hole of the inner tube is arranged along the tangential direction of the inner wall of the inner tube, an opening with the inner diameter of the inner tube and the like is formed in the bottom of the inner tube, a conical tube is installed at the bottom of the inner tube, the edge of a large caliber of the conical tube is connected with the edge of the opening of the inner tube in a sealing mode, and the bottom of a small caliber of the conical tube is connected with a material collecting tube.
Furthermore, a filtering hole in the side wall of the inner barrel is formed in a position close to the top of the inner barrel, and the end head of a discharge pipe communicated with the inner cavity of the inner barrel extends to the middle upper part of the inner cavity of the inner barrel.
Further, the material collecting barrel is connected with the bottom of the conical barrel through threads.
Further, a sampling pipe for discharging filtrate in the collector is arranged on the side wall of the collector, and a valve is arranged on the sampling pipe.
Further, the filtering device also comprises an ultrasonic oscillator which is used for homogenizing and dispersing the fat tissue digestion mixed liquor in the container.
Compared with the prior art, the invention has the beneficial effects that: the fat tissue digestion mixed liquor is pre-separated by the separating cylinder, fat tissue and other large-particle impurities in the fat tissue digestion mixed liquor are removed, and the problem that the filtering speed is influenced due to the blockage of the sieve pores of the cell sieve is solved, so that the filtering efficiency is improved; and further filtering the fat tissue digestion mixed liquor by using a cell sieve, and filtering mixed cells such as red blood cells and the like in the pre-separation filtrate, so that the subsequent centrifugal separation and purification of fat active cells are facilitated.
Drawings
FIG. 1 is a schematic structural diagram of an autologous fat-activated cell filtration device for plastic surgery in an embodiment;
FIG. 2 is a schematic diagram showing the connection of the autologous fat-activated cell filtration device for plastic surgery in the embodiment
FIG. 3 isbase:Sub>A schematic view of the structure of section A-A in FIG. 1;
wherein: 1. a container; 2. screening cells; 3. a collector; 4. a top cover; 5. filtering the screen; 6. a filter cartridge; 7. a collecting funnel; 8. a separation cylinder; 9. an inner barrel; 10. an outer cylinder; 11. a feed pipe; 12. a filtration pore; 13. a discharge pipe; 14. an air exhaust pipe; 15. a tapered barrel; 16. a material collecting barrel; 17. a sampling tube; 18. a valve; 19. an ultrasonic oscillator.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail below with reference to examples and accompanying drawings, and the exemplary embodiments and descriptions thereof are only used for explaining the present invention and are not meant to limit the present invention.
Example (b):
referring to fig. 1 and 2, the autologous fat active cell filtering device for plastic surgery comprises a container 1 for containing the fat tissue digestion mixed liquor, a cell sieve 2 for filtering the fat tissue digestion mixed liquor and a collector 3 for collecting filtrate, wherein the collector 3 is provided with a top cover 4 capable of sealing the collector 3; the cell sieve 2 comprises a filter cylinder 6 with a filter screen 5 at the sealed bottom of the upper part, the bottom of the filter cylinder 6 is connected with a flow collecting funnel 7, the edge of a large caliber of the flow collecting funnel 7 is hermetically connected with the edge of the bottom of the filter cylinder 6, and the end of a small caliber of the flow collecting funnel 7 is communicated with the inner cavity of the collector 3 through a connecting pipeline; the filtering device also comprises a separating cylinder 8 for pre-separating the fat tissue digestion mixed liquor; the separating cylinder 8 is of a double-layer structure and comprises an inner cylinder 9 and an outer cylinder 10, sealing covers are arranged at the bottoms and the tops of the inner cylinder 9 and the outer cylinder 10, a feeding pipe 11 is connected to the wall of the outer cylinder 10, one end of the feeding pipe 11 extends into the container 1, and the other end of the feeding pipe 11 is communicated with an inner cavity of the outer cylinder 10 of the filtering cylinder 6; the wall of the inner barrel 9 is provided with a plurality of filtering holes 12, the inner barrel 9 is connected with a discharge pipe 13 communicated with the inner cavity of the inner barrel 9, and the discharge end of the discharge pipe 13 is communicated with the inner cavity of the cell sieve 2; an exhaust tube 14 is arranged at the top of the collector 3, one end of the exhaust tube 14 is communicated with the inner cavity of the collector 3, and the other end of the exhaust tube is connected with an exhaust pump.
In this embodiment, the preparation method of the mixed liquid for adipose tissue digestion comprises: cleaning and shearing adipose tissues extracted from a human body, and repeatedly washing the adipose tissues with a PBS (phosphate buffer solution) to remove impurities such as blood stains or oil drops; adding 0.2% collagenase 2-3 times the volume of the cut and washed adipose tissues, shaking, mixing, digesting at 37 deg.C for 60min, and stopping digestion with DMEM/F-12 medium containing 10% FBS to obtain an adipose tissue digestion mixture mainly containing impurities such as fat active cells, undigested adipose tissues and red blood cells with a volume larger than that of the fat active cells.
Transferring the fat tissue digestion mixed liquor into a container 1, starting a suction pump (not shown in the figure), generating negative pressure in a collector 3, and allowing the fat tissue digestion mixed liquor in the container 1 to enter a separation cylinder 8 through a feed pipe 11 for pre-separation under the action of atmospheric pressure difference; in the separating cylinder 8, the fat tissue digestion mixed liquid firstly enters an interlayer between the inner cylinder 9 and the outer cylinder 10 and then enters the inner cylinder 9 through the filtering holes 12 on the side wall of the inner cylinder 9; under the filtering action of the filtering holes 12 on the side wall of the inner cylinder 9, most of the fat tissue digestion mixed liquor which is not completely digested is blocked in an interlayer between the inner cylinder 9 and the outer cylinder 10, so that fat tissue and other large-particle impurities in the fat tissue digestion mixed liquor are removed, the problem that the filtering speed is influenced by the blockage of the sieve holes of the cell sieve 2 is avoided, and the filtering efficiency is improved. Pre-separation filtrate entering the inner barrel 9 enters the filter cartridge 6 through the discharge pipe 13, is filtered by the filter screen 5 at the bottom of the filter cartridge 6, and is drained to the collector 3 through the collecting funnel 7; because the diameter of the mixed cells such as the red blood cells in the pre-separation filtrate is larger than that of the fat active cells, the mixed cells such as the red blood cells in the pre-separation filtrate are filtered out by the filter screen 5, so that the subsequent centrifugal separation and purification of the fat active cells are facilitated. Meanwhile, the negative pressure generated by air suction of the air suction pump is adopted for active filtration in the whole filtration process, so that the filtration speed is further improved.
Referring to fig. 1-3, the inner cylinder 9 is a cyclone cylinder, each filtering hole 12 of the inner cylinder 9 is arranged along the tangential direction of the inner wall of the inner cylinder 9, the bottom of the inner cylinder 9 is provided with an opening with the same inner diameter as the inner cylinder 9, the bottom of the inner cylinder 9 is provided with a conical cylinder 15, the edge of the large caliber of the conical cylinder 15 is hermetically connected with the edge of the opening of the inner cylinder 9, and the bottom of the small caliber of the conical cylinder 15 is connected with a material collecting cylinder 16. The pre-separation filtrate entering the interlayer between the outer cylinder 10 and the inner cylinder 9 can carry fat tissues or particles with particle diameters smaller than the diameters of the filter holes 12 when passing through the filter holes 12 on the side wall of the inner cylinder 9, because each filter hole 12 of the filter holes 12 is arranged along the tangential direction of the inner wall of the inner cylinder 9, the inner cylinder 9 forms a cyclone cylinder, the pre-separation filtrate enters the inner cylinder 9 along the tangential direction of the inner wall of the inner cylinder 9 and forms a cyclone in the inner cylinder 9, the large-particle fat tissues entering the inner cylinder 9 along with the pre-separation filtrate rotate adherent to the inner wall of the inner cylinder 9 under the action of centrifugal force due to larger mass and gradually move towards the bottom of the inner cylinder 9 under the action of gravity, and finally enter the material collecting cylinder 16 through the conical cylinder 15, so that the separation of the fat tissues or particles carried by the pre-separation filtrate is realized, and the content of the particles in the filtrate entering the cell sieve 2 is further reduced.
The filtering holes 12 on the side wall of the inner barrel 9 are arranged at a position close to the top of the inner barrel 9, the end of a discharge pipe 13 communicated with the inner cavity of the inner barrel 9 extends to the middle upper part of the inner cavity of the inner barrel 9, pre-separation filtrate entering the inner barrel 9 from the filtering holes 12 on the side wall of the inner barrel 9 forms a rotational flow at the top of the inner barrel 9, and the pre-separation filtrate is gradually pushed downwards as fat tissue digestion mixed liquid gradually enters to form the pre-separation filtrate, wherein large-particle fat tissues or other particle impurities rotate downwards under the action of gravity, upper thrust and centrifugal force; because the end head of the discharge pipe 13 extends to the middle upper part of the inner cavity of the inner barrel 9, the pre-separation filtrate has enough time to do rotational flow movement before entering the discharge pipe 13 and can not be directly discharged from the discharge pipe 13.
In this embodiment, the feeding pipe 11 is also arranged along the tangential direction of the inner wall of the outer cylinder 10, so that the problem of convection generated when the fat tissue digestion mixed liquid directly enters along the radial direction of the inner cylinder 9 is avoided, and thus the fluid resistance and the energy consumption are reduced.
The inner cylinder 9 adopted in the embodiment is a small liquid cyclone separation cylinder and is used for removing micro-particles with the particle size of tens of microns in liquid, the inner diameter of the inner cylinder 9 is 25mm, the height-diameter ratio of the inner cylinder is 2.5, the diameter of the discharge pipe 13 is 3mm, the cyclone separation of a small amount of adipose tissue digestion mixed liquid is met, and more fat cannot be wasted in the cyclone separation cylinder.
The collecting barrel 16 is connected with the bottom of the conical barrel 15 through threads, and can be removed by rotating the collecting barrel 16 after the filtration is finished, so that adipose tissues or particles in the collecting barrel are removed, and cleanness and sanitation are guaranteed.
The side wall of the collector 3 is provided with a sampling pipe 17 for discharging the filtrate in the collector 3, and the sampling pipe 17 is provided with a valve 18. After or during the filtration, the valve 18 can be opened to take out the filtrate for subsequent centrifugal separation and purification or sampling analysis.
The filtering screen 5 of the cell screen 2 can effectively filter mixed cells such as red blood cells and the like in the pre-separated filtrate, ensure the purity of the fat active cells and facilitate the subsequent cell culture of the fat active cells.
The filtering device also comprises an ultrasonic oscillator 19 which is used for homogenizing and dispersing the fat tissue digestion mixed liquor in the container 1. Arrange container 1 in ultrasonic oscillator 19, can fully break up the homogenization with the adipose tissue digestion mixed liquid in container 1 at the filtering process, reduce the pile up of large granule adipose tissue, avoid producing the problem of jam in the filtering process easily, and then guarantee the normal filtration of adipose tissue digestion mixed liquid. Meanwhile, the ultrasonic oscillator 19 can also be used in the process of digesting adipose tissues, so that the digestion speed of the adipose tissues is increased, the digestion efficiency of the adipose tissues is improved, and the content of large granular adipose tissues which are not digested is reduced.
The above is an embodiment of the present invention. The embodiments and specific parameters in the embodiments are only for the purpose of clearly illustrating the verification process of the invention and are not intended to limit the scope of the invention, which is defined by the claims, and all equivalent structural changes made by using the contents of the specification and the drawings of the present invention should be covered by the scope of the present invention.
Claims (5)
1. The utility model provides a plastic cosmetic is with active cell filter equipment of autologous fat, includes container (1) that is used for holding adipose tissue digestion mixed liquid, is used for filtering the cell sieve (2) of adipose tissue digestion mixed liquid and is used for collecting collector (3) of filtrating, its characterized in that: the collector (3) is provided with a top cover (4) capable of sealing the collector (3); the cell sieve (2) comprises a filter cylinder (6) with a cover at the upper part and a filter screen (5) at the bottom, the bottom of the filter cylinder (6) is connected with a flow collecting funnel (7), the edge of a large caliber of the flow collecting funnel (7) is hermetically connected with the edge of the bottom of the filter cylinder (6), and the end of a small caliber of the flow collecting funnel (7) is communicated with the inner cavity of the collector (3) through a connecting pipeline; the filtering device also comprises a separating cylinder (8) for pre-separating the fat tissue digestion mixed liquor; the separation cylinder (8) is of a double-layer structure and comprises an inner cylinder (9) and an outer cylinder (10), sealing covers are arranged at the bottoms and the tops of the inner cylinder (9) and the outer cylinder (10), a feeding pipe (11) is connected to the wall of the outer cylinder (10), one end of the feeding pipe (11) extends into the container (1), and the other end of the feeding pipe (11) is communicated with an inner cavity of the outer cylinder (10) of the filter cylinder (6); the wall of the inner barrel (9) is provided with a plurality of filtering holes (12), the inner barrel (9) is connected with a discharge pipe (13) communicated with the inner cavity of the inner barrel (9), and the discharge end of the discharge pipe (13) is communicated with the inner cavity of the cell sieve (2); an air exhaust pipe (14) is installed at the top of the collector (3), one end of the air exhaust pipe (14) is communicated with the inner cavity of the collector (3), and the other end of the air exhaust pipe (14) is used for being connected with an air exhaust pump;
inner tube (9) are a whirl section of thick bamboo, the inner wall tangential direction setting along inner tube (9) is crossed in every filtration pore (12) of inner tube (9), the bottom of inner tube (9) is provided with the opening big such as with inner tube (9) internal diameter, a toper section of thick bamboo (15) is installed to inner tube (9) bottom, the heavy-calibre edge of toper section of thick bamboo (15) and the opening edge sealing connection of inner tube (9), the small-bore bottom of toper section of thick bamboo (15) is connected with an album feed cylinder (16).
2. The filtration device of claim 1, wherein: the filtering hole (12) on the side wall of the inner barrel (9) is arranged at a position close to the top of the inner barrel (9), and the end of a discharge pipe (13) communicated with the inner cavity of the inner barrel (9) extends to the middle upper part of the inner cavity of the inner barrel (9).
3. The filtration device of claim 1, wherein: the material collecting barrel (16) is connected with the bottom of the conical barrel (15) through threads.
4. The filtration device of claim 1, wherein: the lateral wall of collector (3) is provided with sampling tube (17) that is used for discharging filtrating in collector (3), be provided with valve (18) on sampling tube (17).
5. The filtering device according to any one of claims 1 to 4, wherein: the device also comprises an ultrasonic oscillator (19) which is used for homogenizing and dispersing the mixed liquid for digesting the adipose tissues in the container (1).
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| CN201910516003.3A CN110283719B (en) | 2019-06-14 | 2019-06-14 | Autologous fat active cell filtering device for plastic cosmetology |
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| CN201910516003.3A CN110283719B (en) | 2019-06-14 | 2019-06-14 | Autologous fat active cell filtering device for plastic cosmetology |
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| CN111040930A (en) * | 2020-01-07 | 2020-04-21 | 张军波 | Self-body fat active cell filtering device for plastic and cosmetology |
| CN112358956A (en) * | 2020-09-29 | 2021-02-12 | 程卫华 | Organic solid waste biological fermentation drying system |
| CN112375654B (en) * | 2020-11-20 | 2023-12-05 | 广州穗阳生物学研究有限公司 | Adipose-derived stem cell separation device and separation method |
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| WO2012122603A1 (en) * | 2011-03-15 | 2012-09-20 | Regeneus Pty Ltd | Cell processing method and device |
| CN103055627A (en) * | 2013-01-24 | 2013-04-24 | 中国石油大学(北京) | Straight-flow type pre-separating device for high temperature gas filtration and filter of device |
| CN106190967A (en) * | 2016-07-19 | 2016-12-07 | 安徽惠恩生物科技股份有限公司 | A kind of autologous fat derived stem cell is for the preparation method of beautifying and antisenility |
| CN106434536A (en) * | 2016-08-10 | 2017-02-22 | 何祖平 | Three-dimensional inducing method for inducing spermatogonia stem cell to differentiate into functional sperm cell in vitro |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2009073724A1 (en) * | 2007-12-04 | 2009-06-11 | Ingeneron, Inc. | Apparatus and methods for cell isolation |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012122603A1 (en) * | 2011-03-15 | 2012-09-20 | Regeneus Pty Ltd | Cell processing method and device |
| CN103055627A (en) * | 2013-01-24 | 2013-04-24 | 中国石油大学(北京) | Straight-flow type pre-separating device for high temperature gas filtration and filter of device |
| CN106190967A (en) * | 2016-07-19 | 2016-12-07 | 安徽惠恩生物科技股份有限公司 | A kind of autologous fat derived stem cell is for the preparation method of beautifying and antisenility |
| CN106434536A (en) * | 2016-08-10 | 2017-02-22 | 何祖平 | Three-dimensional inducing method for inducing spermatogonia stem cell to differentiate into functional sperm cell in vitro |
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