CN211005367U - Umbilical cord stem cell separation equipment - Google Patents
Umbilical cord stem cell separation equipment Download PDFInfo
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- CN211005367U CN211005367U CN201920652888.5U CN201920652888U CN211005367U CN 211005367 U CN211005367 U CN 211005367U CN 201920652888 U CN201920652888 U CN 201920652888U CN 211005367 U CN211005367 U CN 211005367U
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- umbilical cord
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Abstract
The utility model provides an umbilical cord stem cell separation device, which relates to the technical field of stem cell separation, and comprises a support frame, a crushing filter box, a separation box and a heat preservation chamber, wherein the separation box is fixedly connected below the crushing filter box; the crushing filter box is of a cylindrical structure, the periphery of the bottom of the crushing filter box inclines towards the center, a first discharging pipe is formed in the center of the bottom of the crushing filter box, the first discharging pipe extends out of the bottom and is fixedly connected with the separation box, a fixing hole is further formed in the top of the crushing filter box, and the crushing filter box is connected with a box cover through the fixing hole. The method is used for solving the problems of complex equipment structure, high price, difficult cleaning and low survival rate of the extracted umbilical cord stem cells in the prior art.
Description
Technical Field
The utility model relates to a stem cell separation technical field especially relates to an umbilical cord stem cell splitter.
Background
The umbilical cord stem cell is a multifunctional stem cell existing in umbilical cord tissues of newborns, can be differentiated into a plurality of tissue cells, and has wide clinical application prospect. The umbilical cord mesenchymal stem cells have high differentiation potential and can be differentiated in multiple directions. It has wide clinical application prospect in the aspects of tissue engineering such as bones, cartilages, muscles, tendons, ligaments, nerves, livers, endothelia, cardiac muscles and the like.
The existing umbilical cord stem cell separation method comprises a density gradient centrifugation technology, a selective cell agglutination method and a method for separating and purifying cells by using cell surface markers, wherein the method for separating and purifying cells by using the cell surface markers separates different types of cells by using the specificity and diversity of monoclonal antibodies and using the reasonable combination of different antibodies, and also comprises a cell dissolution method, a flow cytometry sorting method, a plane adhesion separation method and an immunomagnetic bead separation method; among the above umbilical cord stem cell separation methods, the method of separating and purifying cells using cell surface markers has more application potential due to high separation purity and good separation effect, but most separation methods require expensive instruments or complicated steps and are not easy to popularize.
In the prior art, the device for extracting the stem cells has a complex structure, large volume and high price, and because the cutting tool is overlarge and the tissue volume is small, the stem cells are difficult to uniformly cut in the shearing process of the tissue, a large amount of cells are damaged, and the survival rate of the stem cells of the umbilical cord is reduced; meanwhile, the device is of an integrated structure, so that the cleaning is difficult, and the tissue is easy to remain on the cutter to influence the next use.
SUMMERY OF THE UTILITY MODEL
In view of the above shortcoming of the prior art, the utility model aims to provide an umbilical cord stem cell splitter for solve among the prior art equipment structure complicacy, the price is high, difficult washing, the problem that the umbilical cord stem cell survival rate that extracts is low.
In order to achieve the above and other related objects, the present invention provides an umbilical cord stem cell separation device, which comprises a support frame, a crushing filter box, a separation box and a heat preservation chamber, wherein the separation box is fixedly connected below the crushing filter box, the heat preservation chamber is fixedly arranged outside the crushing filter box and the separation box, and a chamber is formed between the separation box and the crushing filter box, the chamber is used for containing warm water, and the support frame is erected outside the heat preservation chamber;
the crushing and filtering box is of a cylindrical structure, the periphery of the bottom of the crushing and filtering box inclines towards the center, a first discharging pipe is formed in the center of the bottom of the crushing and filtering box, the first discharging pipe extends out of the bottom and is fixedly connected with the separation box, a fixing hole is formed in the top of the crushing and filtering box, the crushing and filtering box is connected with a box cover through the fixing hole, and a first feeding port, an air inlet, a rotating motor and a fixing column used for being connected with the fixing hole are formed in the box cover; the crushing filter box is internally provided with a crushing barrel, and the crushing barrel is connected with the rotating motor through a rotating shaft.
In an embodiment of the utility model, be formed with the cavity between crushing barrel and the crushing rose box, crushing barrel is the tubular structure, and its top is provided with the annular through-hole that is used for throwing the material, and its bottom periphery inclines to the center to form the second discharging pipe at the bottom center, the bottom and the section of thick bamboo wall of crushing barrel are first filter screen, be provided with a plurality of cutting knives along the axial on the crushing barrel inner wall.
In an embodiment of the utility model, the second discharging pipe is provided with the internal thread and extends the bottom of crushing barrel, second discharging pipe one end is provided with the second filter screen, and other end threaded connection has a sealed lid that has the external screw thread, pivot one end with second filter screen fixed connection, the other end with rotate motor fixed connection.
In an embodiment of the utility model, the separation box top be provided with the second pan feeding mouth that first discharging pipe is connected, its bottom activity has cup jointed the collecting box that is used for collecting impurity, the inside three-layer filter membrane that is provided with of separation box is first filtration membrane, second filtration membrane and third filtration membrane from the top down in proper order.
In an embodiment of the present invention, the diameter of the filtering hole of the first filtering net is 0.75-0.5mm, the diameter of the filtering hole of the second filtering net is 0.5-1.0cm, the diameters of the filtering holes of the first filtering net and the second filtering net are 220-350nm, and the diameter of the filtering hole of the third filtering net is 400-500 nm.
In an embodiment of the present invention, the inside of the crushing filter box is further provided with a temperature detector.
In an embodiment of the present invention, the heat preservation chamber is provided with a water inlet and a water outlet.
As described above, the utility model discloses following beneficial effect has:
1. in this embodiment, because a crushing section of thick bamboo and rotation motor fixed connection, case lid and crushing rose box swing joint, consequently a crushing section of thick bamboo can be followed and smashed in the rose box and taken out, unscrew sealed lid, rinse it alone after discharging impurity, be favorable to clearing up the tissue on the cutting knife clean.
2. In this embodiment be provided with a plurality of cutting knives in the crushing section of thick bamboo, the cutting knife volume is less, can be by even cutting after the tissue gets into crushing section of thick bamboo to the protection tissue is not damaged, improves its survival rate.
3. The utility model discloses in all be provided with the heat preservation room in the outside of smashing rose box and separator box, the indoor warm water that is equipped with of heat preservation for the temperature control in will smashing rose box and separator box improves umbilical cord stem cell's survival rate at certain within range.
Drawings
Fig. 1 is a schematic structural diagram disclosed in an embodiment of the present invention.
Fig. 2 is a schematic view showing an external structure of a milling drum disclosed in an embodiment of the present invention.
Fig. 3 is a schematic view showing an internal structure of the crushing cylinder disclosed in the embodiment of the present invention.
Fig. 4 is a schematic view of the case cover disclosed in the embodiment of the present invention.
Fig. 5 is a schematic view of the sealing cover disclosed in the embodiment of the present invention.
Description of the element reference numerals
1-a support frame; 2-crushing and filtering box; 3-a separation box; 4-a heat preservation chamber; 5-a first discharge pipe; 6-fixing holes; 7-a first feeding port; 8-an air inlet; 9-rotating the motor; 10-fixing columns; 11-a crushing cylinder; 12-a ring-shaped through hole; 13-a second discharge pipe; 14-a cutting knife; 15-sealing cover; 16-a second feeding port; 17-a collection box; 18-a first filter membrane; 19-a second filter membrane; 20-a third filtration membrane; 21-a temperature detector; 22-a water inlet; 23-a water outlet; 24-a box cover; 25-a rotating shaft; 26-a first filter screen; 27-a second filter.
Detailed Description
The following description is provided for illustrative purposes, and other advantages and features of the present invention will become apparent to those skilled in the art from the following detailed description.
Please refer to fig. 1 to 5. It should be understood that the structure, ratio, size and the like shown in the drawings attached to the present specification are only used for matching with the content disclosed in the specification, so as to be known and read by those skilled in the art, and are not used for limiting the limit conditions that the present invention can be implemented, so that the present invention has no technical essential meaning, and any structure modification, ratio relationship change or size adjustment should still fall within the scope that the technical content disclosed in the present invention can cover without affecting the function that the present invention can produce and the purpose that the present invention can achieve. Meanwhile, the terms such as "upper", "lower", "left", "right", "middle" and "one" used in the present specification are for convenience of description, and are not intended to limit the scope of the present invention, and changes or adjustments of the relative relationship thereof may be made without substantial technical changes, and the present invention is also regarded as the scope of the present invention.
Referring to fig. 1-5, the present invention provides an umbilical cord stem cell separation device, which comprises a support frame 1, a crushing filter box 2, a separation box 3 and a heat preservation chamber 4, wherein the separation box 3 is fixedly connected below the crushing filter box 2, the heat preservation chamber 4 is fixedly arranged outside the crushing filter box 2 and the separation box 3, and a chamber is formed between the heat preservation chamber 4 and the crushing filter box 2 and the separation box 3, the chamber is used for holding warm water, and the support frame 1 is erected outside the heat preservation chamber 4;
the crushing and filtering box 2 is of a cylindrical structure, the periphery of the bottom of the crushing and filtering box is inclined towards the center, a first discharging pipe 5 is formed in the center of the bottom, the first discharging pipe 5 extends out of the bottom and is fixedly connected with the separation box 3, a fixing hole 6 is further formed in the top of the crushing and filtering box 2, the crushing and filtering box 2 is connected with a box cover 24 through the fixing hole 6, and a first feeding hole 7, an air inlet 8, a rotating motor 9 and a fixing column 10 used for being connected with the fixing hole 6 are formed in the box cover 24; the crushing barrel 11 is arranged inside the crushing filter box 2, and the crushing barrel 11 is connected with the rotating motor 9 through a rotating shaft 25.
In this embodiment, the box cover 24 is provided with fixing posts 10, and the fixing posts 10 can be embedded into the fixing holes 6 and used for fixing the box cover 24 on the top of the pulverizing and filtering box 2; the periphery of the bottom of the crushing filter box 2 is obliquely and centrally arranged, so that the materials in the box can be favorably concentrated into the first discharge pipe 5; the air inlet 8 is used for introducing oxygen into the crushing filter box 2 to ensure the survival rate of tissues.
Based on the above embodiment, a cavity is formed between the crushing cylinder 11 and the crushing filter box 2, the crushing cylinder 11 is of a cylindrical structure, the top of the crushing cylinder is provided with an annular through hole 12 for feeding, the periphery of the bottom of the crushing cylinder inclines towards the center, a second discharge pipe 13 is formed in the center of the bottom of the crushing cylinder, the bottom and the cylinder wall of the crushing cylinder 11 are both provided with a first filter screen 26, and the inner wall of the crushing cylinder 11 is provided with a plurality of cutting knives 14 along the axial direction.
Specifically, the second discharging pipe 13 is provided with an internal thread and extends out of the bottom of the crushing barrel 11, one end of the second discharging pipe 13 is provided with a second filter screen 27, the other end of the second discharging pipe is in threaded connection with a sealing cover 15 with an external thread, one end of the rotating shaft 25 is fixedly connected with the second filter screen 27, and the other end of the rotating shaft is fixedly connected with the rotating motor 9.
Specifically, the diameter of the filter hole of the first filter 26 is 0.75-0.5mm, and the diameter of the filter hole of the second filter 27 is 0.5-1.0 cm.
In this embodiment, the annular through hole 12 is disposed corresponding to the first feeding port 7, and is used for feeding the tissue, the enzyme and the buffer solution into the crushing cylinder 11, the crushing cylinder 11 is fixedly connected to the rotating motor 9, the rotating motor 9 drives the crushing cylinder 11 to rotate, the tissue is uniformly cut by the cutting knife 14, and the umbilical cord stem cells with smaller diameter and other liquid impurities enter the crushing filter box through the first filter screen;
the impurities with larger diameter are retained in the crushing cylinder 11, and because the crushing cylinder 11 is fixedly connected with the rotating motor 9, and the box cover 24 is movably connected with the crushing filter box 2, the crushing cylinder 11 can be taken out from the crushing filter box 2, the sealing cover 15 is unscrewed, and the impurities are cleaned independently after being discharged, which is beneficial to cleaning the tissues on the cutting knife 14;
when the sealing cap 15 is inserted into the second outlet pipe 13, a second filter 27 arranged at the end of the second outlet pipe 13 can be sealed, which ensures that the second outlet pipe 13 is in a sealed state during the tissue cutting process.
Based on the above embodiment, the top of the separation box 3 is provided with a second feeding port 16 connected with the first discharging pipe 5, the bottom of the separation box is movably sleeved with a collecting box 17 for collecting impurities, three layers of filter membranes are arranged in the separation box 3, and the first filter membrane 18, the second filter membrane 19 and the third filter membrane 20 are arranged in sequence from top to bottom;
specifically, the filter pore diameters of the first filter membrane 18 and the second filter membrane 19 are 220-350nm, and the filter pore diameter of the third filter membrane 20 is 400-500 nm.
In this embodiment, as known in the art, according to the research of Rodriguez, ane-Marie, etc., it is indicated that the umbilical cord stem cells exhibit cell surface markers (cell surface markers) such as CD73, CD90, CD105, etc.;
the first filter membrane 18, the second filter membrane 19 and the third filter membrane 20 are therefore coated with a layer of umbilical cord stem cell-specific capture antibody selected from the group consisting of: CD73, CD90, CD105, and other combinations.
The umbilical cord stem cells and other liquid impurities flow through the first filtering membrane 18, the second filtering membrane 19 and the third filtering membrane 20 in sequence after entering the separation box, tissue fluid, blood water and other cells can directly enter the collection box 17 through the filtering membranes, and the cells with specific cell markers identified by the antibodies are captured by the antibodies on the three filtering membranes, so that the cells (including the umbilical cord stem cells to be indicated) showing the specific cell markers are attached to the filtering membranes, finally the collection box 17 is taken down, and then the three filtering membranes are taken out.
Based on the above embodiment, the crushing filter box 2 is also provided with a temperature detector 21 inside.
Based on the above embodiment, the incubation chamber 4 is provided with a water inlet 22 and a water outlet 23.
In this embodiment, the water inlet 22 is used for adding hot water into the heat preservation chamber 4, so as to ensure that the temperature in the crushing and filtering box and the separating box 3 is maintained within a certain range, which is beneficial to the survival of umbilical cord stem cells, and the water outlet 23 is used for discharging water in the heat preservation chamber.
The using method comprises the following steps: when the device is used, the box cover 24 is arranged at the top of the crushing and filtering box 2, the fixing column 10 is embedded into the fixing hole 6 and is used for fixing the box cover 24 and the crushing barrel 11, tissues, enzymes and buffer solution are put into the crushing barrel 11 from the first feeding port 7, the rotary motor 3 drives the crushing barrel 11 to rotate and is used for crushing the tissues, and umbilical cord stem cells with smaller diameter and other liquid impurities enter the crushing and filtering box 2 through the first filter screen 18; the impurities with larger diameter are retained in the crushing barrel 11, are discharged from the second discharge pipe 13 of the crushing barrel 11 and enter the separation box 3, tissue fluid, blood water and other cells can directly enter the collection box 17 through the three-layer filtering membrane, the cells with specific cell markers identified by the antibodies are captured by the antibodies on the three-layer filtering membrane, finally the collection box 17 is taken down, then the three-layer filtering membrane is taken out, and the umbilical cord stem cells on the filtering membrane are extracted.
To sum up, the utility model has the advantages of simple structure, reasonable in design, it is efficient. Therefore, the utility model effectively overcomes various defects in the prior art and has high industrial utilization value.
The above embodiments are merely illustrative of the principles and effects of the present invention, and are not to be construed as limiting the invention. Modifications and variations can be made to the above-described embodiments by those skilled in the art without departing from the spirit and scope of the present invention. Accordingly, it is intended that all equivalent modifications or changes which may be made by those skilled in the art without departing from the spirit and technical spirit of the present invention be covered by the claims of the present invention.
Claims (7)
1. The umbilical cord stem cell separation equipment is characterized by comprising a support frame (1), a crushing filter box (2), a separation box (3) and a heat preservation chamber (4), wherein the separation box (3) is fixedly connected below the crushing filter box (2), the heat preservation chamber (4) is fixedly arranged outside the crushing filter box (2) and the separation box (3), a containing chamber is formed between the heat preservation chamber and the crushing filter box (2) and the separation box (3), the containing chamber is used for containing warm water, and the support frame (1) is erected outside the heat preservation chamber (4);
the crushing and filtering box (2) is of a cylindrical structure, the periphery of the bottom of the crushing and filtering box inclines towards the center, a first discharging pipe (5) is formed in the center of the bottom of the crushing and filtering box, the first discharging pipe (5) extends out of the bottom and is fixedly connected with the separation box (3), a fixing hole (6) is further formed in the top of the crushing and filtering box (2), the crushing and filtering box (2) is connected with a box cover (24) through the fixing hole (6), and a first feeding hole (7), an air inlet (8), a rotating motor (9) and a fixing column (10) used for being connected with the fixing hole (6) are formed in the box cover (24); the crushing filter box (2) is internally provided with a crushing barrel (11), and the crushing barrel (11) is connected with the rotating motor (9) through a rotating shaft (25).
2. The umbilical cord stem cell separation device of claim 1, wherein: the grinding cylinder (11) and the grinding filter box (2) are formed with a cavity, the grinding cylinder (11) is of a cylindrical structure, the top of the grinding cylinder is provided with an annular through hole (12) used for feeding, the periphery of the bottom of the grinding cylinder inclines towards the center, a second discharging pipe (13) is formed in the center of the bottom of the grinding cylinder, the bottom and the cylinder wall of the grinding cylinder (11) are both provided with a first filter screen (26), and a plurality of cutting knives (14) are arranged on the inner wall of the grinding cylinder (11) along the axial direction.
3. The umbilical cord stem cell separation device of claim 2, wherein: second discharging pipe (13) are provided with the internal thread and extend the bottom of smashing a section of thick bamboo (11), second discharging pipe (13) one end is provided with second filter screen (27), and other end threaded connection has a sealed lid (15) that has the external screw thread, pivot (25) one end with second filter screen (27) fixed connection, the other end with rotate motor (9) fixed connection.
4. The umbilical cord stem cell separation device of claim 3, wherein: the separation box is characterized in that a second feeding port (16) connected with the first discharging pipe (5) is arranged at the top of the separation box (3), a collecting box (17) used for collecting impurities is movably sleeved at the bottom of the separation box, three layers of filter membranes are arranged inside the separation box (3), and a first filter membrane (18), a second filter membrane (19) and a third filter membrane (20) are sequentially arranged from top to bottom.
5. The umbilical cord stem cell separation device of claim 4, wherein: the diameter of the filter hole of the first filter screen (26) is 0.75-0.5mm, the diameter of the filter hole of the second filter screen (27) is 0.5-1.0cm, the diameters of the filter holes of the first filter screen (18) and the second filter screen (19) are 220-350nm, and the diameter of the filter hole of the third filter screen (20) is 400-500 nm.
6. The umbilical cord stem cell separation device of claim 1, wherein: and a temperature detector (21) is also arranged in the crushing and filtering box (2).
7. The umbilical cord stem cell separation device of claim 1, wherein: the heat preservation chamber (4) is provided with a water inlet (22) and a water outlet (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920652888.5U CN211005367U (en) | 2019-05-08 | 2019-05-08 | Umbilical cord stem cell separation equipment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920652888.5U CN211005367U (en) | 2019-05-08 | 2019-05-08 | Umbilical cord stem cell separation equipment |
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| Publication Number | Publication Date |
|---|---|
| CN211005367U true CN211005367U (en) | 2020-07-14 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920652888.5U Expired - Fee Related CN211005367U (en) | 2019-05-08 | 2019-05-08 | Umbilical cord stem cell separation equipment |
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| CN (1) | CN211005367U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
| CN115322875A (en) * | 2022-08-12 | 2022-11-11 | 中科院南昌高新技术产业协同创新研究院 | Stem cell pretreatment device and method |
-
2019
- 2019-05-08 CN CN201920652888.5U patent/CN211005367U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
| CN115322875A (en) * | 2022-08-12 | 2022-11-11 | 中科院南昌高新技术产业协同创新研究院 | Stem cell pretreatment device and method |
| CN115322875B (en) * | 2022-08-12 | 2023-06-30 | 中科院南昌高新技术产业协同创新研究院 | Stem cell pretreatment device and method |
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Legal Events
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| TR01 | Transfer of patent right |
Effective date of registration: 20200706 Address after: 201306 building C, No. 888, Huanhu West 2nd Road, Lingang New Area, China (Shanghai) pilot Free Trade Zone, Pudong New Area, Shanghai Patentee after: Chengxi (Shanghai) Biotechnology Co.,Ltd. Address before: Building 11, Jinting Fuyuan, Jinting Town, Wuzhong District, Suzhou City, Jiangsu Province Patentee before: Aixin (Suzhou) Life Science Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20200714 Termination date: 20210508 |
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| CF01 | Termination of patent right due to non-payment of annual fee |