CN209669220U - A kind of filter separator for mesenchymal stem cells - Google Patents
A kind of filter separator for mesenchymal stem cells Download PDFInfo
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- CN209669220U CN209669220U CN201920072089.0U CN201920072089U CN209669220U CN 209669220 U CN209669220 U CN 209669220U CN 201920072089 U CN201920072089 U CN 201920072089U CN 209669220 U CN209669220 U CN 209669220U
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- bottle body
- filter
- stem cells
- mesenchymal stem
- air inlet
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Abstract
The utility model discloses a kind of filter separator for mesenchymal stem cells, belong to technical field of cell separation, separator includes separating the upper cover body being arranged on bottle body and separation bottle body, several filter screens of setting in separating bottle body, it separates and wears air inlet pipe I on the side wall of bottle body, product outlet is arranged in the bottom for separating bottle body, and the outlet end of product outlet is arranged lower cover, wears air inlet pipe II and inlet tube in upper cover body.The utility model improves filter effect using double medium filtration web frame, wherein the first filter screen can filter out the biggish tissue of volume, second filter screen can efficiently separate mescenchymal stem cell, filter screen can increase the contact area between tissue and filter screen using inverted conical shape structure, improve filter efficiency;Air inlet pipe I and air inlet pipe II can be such that the air pressure of separating bottle inside and outside is consistent, and prevent separation in bottle body and cause tissue to stop filtration in negative pressure during the filtration process;The setting of product outlet can be improved the speed and collection rate collected after tissue filter.
Description
Technical field
The utility model belongs to technical field of cell separation, and in particular to a kind of filter separator for mesenchymal stem cells.
Background technique
With the deep development of stem cell and tissue engineering technique research, application, the separation preparation of stem cell is used as the neck
One of domain key technology and be paid more and more attention.Separator development for the leucocyte of medical field, tumour cell so far
Comparatively fast, but the separation equipment of mescenchymal stem cell is seldom, and the most mescenchymal stem cell separator of application is cell sieve at present
Net, what is generally selected is 100 mesh~300 mesh stainless steel mesh, reaches cell by rocking, stirring and shake and disappears
The purpose of tissue block separation after change, such as the separation of Human plactnta mescenchymal stem cell are exactly first with collagenase digesting Freshman
Placenta tissue, then the individual cells or cell cluster digested are screened with this stainless steel mesh, it is dry that this method extracts mesenchyma
Cell low efficiency, it is time-consuming and laborious, and it is be easy to cause germ contamination during extracting, influence quality.
Utility model content
The purpose of the utility model is to provide a kind of filter separator for mesenchymal stem cells, to solve the above problems.
In order to achieve the above object, the technical solution adopted in the utility model are as follows:
A kind of filter separator for mesenchymal stem cells including separation bottle body and separates the upper cover body being arranged on bottle body, described
Several filter screens of setting, separate and wear air inlet pipe I on the side wall of bottle body in separating bottle body, and the bottom setting finished product for separating bottle body goes out
Mouthful, lower cover is arranged in the outlet end of product outlet, wears air inlet pipe II and inlet tube in the upper cover body.
The filter screen includes being set in turn in intracorporal first filter screen of separating bottle and the second filter screen from top to bottom, the
One filter screen and the second filter screen such as are at the big inverted conical shape.
The mesh number of second filter screen is 100-300 mesh.The mesh number is not only able to efficiently separate mescenchymal stem cell, and
And it is not easy to plug mesh.
The periphery of the air inlet pipe II is arranged cooling device.Cool down into the intracorporal gas of separating bottle, makes to separate
Lower temperature is kept in bottle body, can effectively improve the survival rate of stem cell.
Sterilizing filter is respectively set in the air inlet pipe I and air inlet pipe II.It reduces bacterium to enter in separating bottle body, reduce
To the probability of tissue pollution.
Deflector is arranged in the bottom of the separation bottle body, and deflector is the reverse frustoconic of upper and lower opening, the top margin of deflector
It is connected with the inner wall for separating bottle body, the bottom edge of deflector and the arrival end of product outlet connect.Filtered group is prevented to be woven in point
From accumulating on the bottom surface of bottle body, cause the waste of tissue and accumulation rotten.
Fixed link, the traversing through end setting vibration motor of fixed link are worn on the outer wall of the separation bottle body, fixed link is worn
Enter end to be in contact with the outer rim of the second filter screen.Help to improve the extraction efficiency of stem cell
Solenoid valve is set on the inlet tube.The flow velocity for facilitating control tissue prevents raw material inflow from excessively entering and caused
The accumulation organized on strainer reduces filter efficiency.
The cooling device includes the spiral cooling tube of shell and enclosure interior setting.
It is threadedly coupled between separation bottle body and the upper cover body, separates and heat preservation is respectively set on bottle body and the inner wall of upper cover body
Layer.Improve sealing effect and heat insulation effect.
The utility model having the beneficial effect that compared to the prior art
The utility model using double medium filtration web frame improve filter effect, wherein the first filter screen can filter out volume compared with
Big tissue, the second filter screen can efficiently separate mescenchymal stem cell, and filter screen can increase group using inverted conical shape structure
The contact area between filter screen is knitted, filter efficiency is improved;Air inlet pipe I and air inlet pipe II can make the gas of separating bottle inside and outside
Pressure is consistent, and is prevented separation in bottle body and is caused tissue to stop filtration in negative pressure during the filtration process;The setting energy of product outlet
Enough improve the speed and collection rate collected after tissue filter.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of the utility model;
Appended drawing reference meaning is as follows: 1, separating bottle body;2, upper cover body;3, filter screen;3-1, the first filter screen;3-2, second
Filter screen;4, air inlet pipe I;5, product outlet;6, lower cover;7, air inlet pipe II;8, inlet tube;9, cooling device;10, degerming
Filter;11, deflector;12, fixed link;13, motor is shaken;14, solenoid valve;15, shell;16, spiral cooling tube;17, it keeps the temperature
Layer.
Specific embodiment
The utility model is described further with reference to the accompanying drawings and detailed description.
As shown in Figure 1, a kind of filter separator for mesenchymal stem cells, including what is be arranged on separation bottle body 1 and separation bottle body 1
Upper cover body 2, the interior several filter screens 3 of setting of the separation bottle body 1, separates and wears air inlet pipe I 4 on the side wall of bottle body 1, separate bottle body
Product outlet 5 is arranged in 1 bottom, and the outlet end of product outlet 5 is arranged lower cover 6, wears air inlet pipe II 7 in the upper cover body 2
With inlet tube 8.
The filter screen 3 includes the filtering of the first filter screen 3-1 and second being set in turn in separation bottle body 1 from top to bottom
Net 3-2, the first filter screen 3-1 and the second filter screen 3-2 be etc. big inverted conical shape.
The mesh number of the second filter screen 3-2 is 100-300 mesh.
The periphery of the air inlet pipe II 7 is arranged cooling device 9.
Sterilizing filter 10 is respectively set in the air inlet pipe I 4 and air inlet pipe II 7.
Deflector 11 is arranged in the bottom of the separation bottle body 1, and deflector 11 is the reverse frustoconic of upper and lower opening, deflector 11
Top margin with separate bottle body 1 inner wall connect, the bottom edge of deflector 11 is connect with the arrival end of product outlet 5.
Fixed link 12 is worn on the outer wall of the separation bottle body 1, the traversing through end setting vibration motor 13 of fixed link 12 is fixed
The penetrating end of bar 12 is in contact with the outer rim of the second filter screen 3-2.
Solenoid valve 14 is set on the inlet tube 8.
The cooling device 9 includes the spiral cooling tube 16 being arranged inside shell 15 and shell 15.
It is threadedly coupled between the separation bottle body 1 and upper cover body 2, separates and be respectively set on bottle body 1 and the inner wall of upper cover body 2
Insulating layer 17.
In use, first tighten upper cover body 2 and the closing separation bottle body 1 of lower cover 6, by air inlet pipe I 4 and air inlet pipe II 7 with it is defeated
Air pipe is connected to and opens the solenoid valve 14 being arranged in air inlet pipe I 4 and air inlet pipe II 7, while conveying into spiral cooling tube 16
Liquid nitrogen cools down cooling device 9 to the gas entered by air inlet pipe II 7, to reduce the temperature in separation bottle body 1, then
The power supply of vibration motor 13 and starting are connected, the raw material that stem cell is extracted in the input of solenoid valve 14 can be started at this time, raw material passes through
First filter screen 3-1 separates the biggish tissue of volume, and the tissue of small volume is filtered off and flow to the second filter screen 3-2
On, mescenchymal stem cell, which is filtered off and passes through deflector 12, under the shock effect of vibration motor 13 is collected at product outlet 5,
I.e. openable lower cover 6 is collected when the mesenchymal stem cells collected at product outlet 5 reach a certain amount of.
Claims (10)
1. a kind of filter separator for mesenchymal stem cells, it is characterised in that: including being set in separation bottle body (1) and separation bottle body (1)
The upper cover body (2) set, the interior several filter screens (3) of setting of the separation bottle body (1), separates and wears air inlet on the side wall of bottle body (1)
Product outlet (5) are arranged in the bottom of pipe I (4), separation bottle body (1), and lower cover (6) are arranged in the outlet end of product outlet (5), described
Air inlet pipe II (7) and inlet tube (8) are worn in upper cover body (2).
2. filter separator for mesenchymal stem cells as described in claim 1, it is characterised in that: the filter screen (3) includes certainly
The first filter screen (3-1) and the second filter screen (3-2) being set in turn under above in separation bottle body (1), the first filter screen (3-
1) and the second filter screen (3-2) be etc. big inverted conical shape.
3. filter separator for mesenchymal stem cells as claimed in claim 2, it is characterised in that: second filter screen (3-2)
Mesh number be 100-300 mesh.
4. filter separator for mesenchymal stem cells as claimed in claim 3, it is characterised in that: outside the air inlet pipe II (7)
It is arranged cooling device (9) week.
5. filter separator for mesenchymal stem cells as claimed in claim 4, it is characterised in that: the air inlet pipe I (4) and air inlet
Sterilizing filter (10) are respectively set in pipe II (7).
6. filter separator for mesenchymal stem cells as claimed in claim 5, it is characterised in that: the bottom of separation bottle body (1)
Portion be arranged deflector (11), deflector (11) be upper and lower opening reverse frustoconic, the top margin of deflector (11) with separate bottle body (1)
Inner wall connection, the bottom edge of deflector (11) is connect with the arrival end of product outlet (5).
7. filter separator for mesenchymal stem cells as claimed in claim 6, it is characterised in that: outside separation bottle body (1)
It is worn on wall fixed link (12), the traversing through end of fixed link (12) setting vibration motor (13), the penetrating end of fixed link (12) and the
The outer rim of two filter screens (3-2) is in contact.
8. filter separator for mesenchymal stem cells as claimed in claim 7, it is characterised in that: be arranged on the inlet tube (8)
Solenoid valve (14).
9. filter separator for mesenchymal stem cells as claimed in claim 8, it is characterised in that: the cooling device (9) includes
The spiral cooling tube (16) being arranged inside shell (15) and shell (15).
10. filter separator for mesenchymal stem cells as claimed in claim 9, it is characterised in that: the separation bottle body (1) and upper
Lid is threadedly coupled between (2), separates and insulating layer (17) are respectively set on the inner wall of bottle body (1) and upper cover body (2).
Priority Applications (1)
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CN201920072089.0U CN209669220U (en) | 2019-01-16 | 2019-01-16 | A kind of filter separator for mesenchymal stem cells |
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CN201920072089.0U CN209669220U (en) | 2019-01-16 | 2019-01-16 | A kind of filter separator for mesenchymal stem cells |
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Cited By (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
CN117025367A (en) * | 2023-08-21 | 2023-11-10 | 江苏康进医疗器材有限公司 | Cell filter bag |
-
2019
- 2019-01-16 CN CN201920072089.0U patent/CN209669220U/en active Active
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN113388506A (en) * | 2021-05-26 | 2021-09-14 | 四川大学华西第二医院 | Automatic mesenchymal stem cell separation device |
CN113174361A (en) * | 2021-06-10 | 2021-07-27 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
CN113174361B (en) * | 2021-06-10 | 2023-11-14 | 南通市第一老年病医院(上海大学附属南通医院、南通市第六人民医院、南通市肺科医院) | Pathological cell separation adsorption type extraction method |
CN117025367A (en) * | 2023-08-21 | 2023-11-10 | 江苏康进医疗器材有限公司 | Cell filter bag |
CN117025367B (en) * | 2023-08-21 | 2024-06-18 | 江苏康进医疗器材有限公司 | Cell filter bag |
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