CN117025367A - Cell filter bag - Google Patents
Cell filter bag Download PDFInfo
- Publication number
- CN117025367A CN117025367A CN202311052353.1A CN202311052353A CN117025367A CN 117025367 A CN117025367 A CN 117025367A CN 202311052353 A CN202311052353 A CN 202311052353A CN 117025367 A CN117025367 A CN 117025367A
- Authority
- CN
- China
- Prior art keywords
- filter screen
- bag body
- bag
- connecting portion
- cell filter
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000926 separation method Methods 0.000 claims abstract description 10
- 238000007789 sealing Methods 0.000 claims description 29
- 238000003756 stirring Methods 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 abstract description 26
- 239000007788 liquid Substances 0.000 abstract description 15
- 239000012535 impurity Substances 0.000 abstract description 6
- 230000000694 effects Effects 0.000 abstract description 3
- 238000001914 filtration Methods 0.000 description 18
- 238000005192 partition Methods 0.000 description 3
- 238000013019 agitation Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 238000003466 welding Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Molecular Biology (AREA)
- Filtration Of Liquid (AREA)
Abstract
The invention relates to the field of biochemical products, in particular to a cell filter bag which comprises a bag body, wherein a plurality of layers of filter screens are arranged in the bag body, the holes of the filter screens of the layers are gradually reduced in the bag body, a liquid inlet pipe is arranged on the bag body and is arranged on one side with larger hole diameter of the filter screen, a liquid outlet pipe is arranged on the bag body and is arranged on one side with smaller hole diameter of the filter screen. The invention has the effects of improving the separation efficiency of impurities in the mixture and reducing the occurrence of the condition that the mixture is polluted by the external environment.
Description
Technical Field
The invention relates to the field of biochemical supplies, in particular to a cell filter bag.
Background
In some biological experiments, there are cases where it is necessary to perform cell separation and pellet or chip removal, and it is common to dispose a sieve on a container such as a test tube, by pouring the mixture into the sieve, separating impurities in the mixture through the sieve, and obtaining a desired residue in the test tube.
But the diameter of container openings such as test tubes is not big, leads to the area of action of the filter screen that sets up in the opening part to be less, appears the filter screen easily to block up, and then has reduced the efficiency to impurity separation in the mixture. Meanwhile, when the filtering is carried out, the environment is generally open, namely, the mouth of the test tube is directly exposed to the external environment, and the screen is also the same, so that the contact time with the external environment is longer due to the low filtering efficiency in the process of filtering the mixture. The external environment is also difficult to form a relatively stable sterile environment, so that the mixture is polluted in the external environment during the filtering process.
Disclosure of Invention
In order to improve the separation efficiency of impurities in the mixture and reduce the occurrence of pollution of the mixture by the external environment, the invention provides a cell filter bag.
The invention provides a cell filter bag, which adopts the following technical scheme:
the utility model provides a cell filter bag, includes the bag body, be equipped with a plurality of layers of filter screen in the bag body, a plurality of layers the hole of filter screen is radial reduce gradually in the bag body, be equipped with the feed liquor pipe on the bag body, the feed liquor pipe sets up the great one side in filter screen aperture, be equipped with the drain pipe on the bag body, the drain pipe sets up one side that the filter screen aperture is less.
In a specific embodiment, the filter mesh is connected with the bag body through a high-frequency heat sealing process.
In a specific embodiment, the bag body is connected with a connecting pipe.
In a specific embodiment, the bag body comprises a connecting portion and a collecting portion, the collecting portion is communicated with the connecting portion, the filter screen is arranged in the connecting portion, and a separation portion is arranged between the connecting portion and the collecting portion.
In a specific implementation, the bag body is provided with a mounting hole, and an agitating assembly is arranged in the mounting hole.
In a specific embodiment, the stirring assembly includes a stirring block, a rotating shaft and a mounting seat, the mounting seat is disposed in the mounting hole, the rotating shaft is disposed in the mounting seat, one end of the rotating shaft is disposed in the connecting portion, the other end of the rotating shaft is disposed outside the connecting portion, and the stirring block is disposed at one end of the rotating shaft in the connecting portion.
In a specific implementation manner, the mounting seat comprises a first connecting plate, a second connecting plate, a top block and a connecting sleeve, wherein the top block is arranged on the first connecting plate, the connecting sleeve is arranged on the second connecting plate, and a guide block is arranged at one end, far away from the first connecting plate, of the top block.
In a specific implementation manner, the bag body further comprises a sealing cover, the sealing cover is arranged on one side, away from the collecting part, of the connecting part, a connecting ring is arranged on the outer wall of the connecting part, one side, away from the sealing cover, of the connecting ring is provided with a clamping ring, a plurality of connecting columns are arranged on the clamping ring, connecting holes for inserting the connecting columns are formed in the sealing cover, a plurality of first yielding holes for inserting the connecting columns are formed in the connecting ring, and a plurality of second yielding holes for inserting the connecting columns are formed in the filter screen.
In summary, the present invention includes at least one of the following beneficial technical effects:
1. through the mode of setting the filter screen in the bag body, enlarged the area of action of filter screen, promoted the filtration efficiency to the mixture. And after the filtering area is enlarged, the occurrence of the condition that a filter screen is blocked can be reduced, and the filtering efficiency of the mixture is further improved.
2. The bag body is sealed in a high-frequency heat sealing mode, so that the contact between the mixture and the external environment is reduced, and the situation that the mixture is polluted by the external environment is avoided.
3. Through setting up stirring subassembly and stirring the mixture in the filter screen, promote the mobility of mixture in the filter screen for the filter screen is difficult for being stopped up more, promotes the filtration efficiency of mixture.
4. And a separation part is arranged between the collection part and the connecting part, after cell filtration is completed, the collection part is sealed by heat sealing or fusing along the separation part, or the collection part is separated from the connecting part after sealing, so that the residues obtained after filtration are conveniently preserved in a sterile way.
Drawings
Fig. 1 is a schematic overall structure of embodiment 1.
Fig. 2 is a sectional view showing the internal structure of the bag body in embodiment 1.
Fig. 3 is a schematic overall structure of embodiment 2.
Fig. 4 is a sectional view showing the internal structure of the bag body in example 2.
FIG. 5 is an exploded view of a structure embodying an agitation assembly
Reference numerals illustrate: 1. a bag body; 11. a connection part; 12. a collection section; 13. sealing cover; 2. a first filter screen; 3. a second filter screen; 4. a liquid inlet pipe; 5. a liquid outlet pipe; 6. a connecting pipe; 7. a clamping ring; 8. a connecting column; 9. an agitation assembly; 91. a rotating shaft; 92. a stirring block; 93. a mounting base; 931. a first connection plate; 932. a second connecting plate; 933. a top block; 934. connecting sleeves; 935. a guide block; 10. a partition portion; 15. a mounting hole; 16. and a connecting ring.
Detailed Description
The invention is described in further detail below with reference to fig. 1-5.
Example 1:
referring to fig. 1 and 2, the cell filter bag comprises a bag body 1, two layers of filter screens are arranged in the bag body 1, and for convenience in description, a first filter screen 2 and a second filter screen 3 are adopted to distinguish below, the first filter screen 2 is arranged above, the second filter screen 3 is arranged below, and the aperture of the first filter screen 2 is larger than that of the second filter screen 3. The volume of the second filter 3 is larger than the volume of the first filter 2. When the two-stage high-frequency heat-seal bag is connected, the first filter screen 2 and the second filter screen 3 are overlapped firstly, so that the edges of the first filter screen 2 and the second filter screen 3 are flush, then the first filter screen 2 and the second filter screen 3 are folded in half, the first filter screen 2 is arranged in the second filter screen 3, the edges of the first filter screen 2 and the second filter screen 3 are connected in a welding mode, the first filter screen 2 and the second filter screen 3 are placed in the bag body 1, the edges of the second filter screen 3 are flush with the edges of the bag body 1, and the bag body 1, the first filter screen and the second filter screen 2 are fixed in a high-frequency heat-seal mode.
Referring to fig. 1 and 2, the top end of the bag body 1 is communicated with a liquid inlet pipe 4, and the bottom end of the bag body 1 is communicated with a liquid outlet pipe 5. The liquid inlet pipe 4 is fixed at the top end of the bag body 1 in a high-frequency heat sealing mode, and the liquid outlet pipe 5 is fixed at the bottom end of the bag body 1 in a high-frequency heat sealing mode.
Referring to fig. 1 and 2, the mixture enters the bag body 1 from the liquid inlet pipe 4, is primarily filtered by the first filter screen 2 to remove part of impurities, and is secondarily and more finely filtered by the second filter screen 3 to improve the impurity removing effect on the mixture. Because the aperture of the second filter screen is smaller, the filtering rate of the mixture is slower, and the volume of the second filter screen 3 is enlarged, so that the mixture can be buffered in the second filter screen 3, and the situation that the mixture in the first filter screen 2 cannot be subjected to subsequent filtering due to the fact that the filtering rate of the second filter screen 3 is slower is reduced.
Referring to fig. 1 and 2, the top end of the bag body 1 is also communicated with two connecting pipes 6, and the two connecting pipes 6 are fixedly connected with the bag body 1 through high-frequency heat sealing. The luer inner cone is arranged on one connecting pipe 6, and the luer outer cone is arranged on the other connecting pipe 6, so that the bag body 1 can be conveniently connected with other equipment or pipelines.
The connecting pipe 6, the liquid inlet pipe 4 and the liquid outlet pipe 5 are respectively provided with a liquid stopping clamp, so that the filtering and conveying processes are conveniently controlled. Can set up the sample trigeminal on feed liquor pipe 4, conveniently carry out needleless application of sample.
Example 2:
referring to fig. 3 and 4, embodiment 2 differs from embodiment 1 in that: the bag body 1 comprises a sealing cover 13, a connecting part 11 and a collecting part 12, wherein the connecting part 11 and the collecting part 12 are integrally formed, and the sealing cover 13 is arranged at one end of the connecting part 11 far away from the collecting part 12. The outer wall of connecting portion 11 integrated into one piece has go up go-between 16, is equipped with clamp ring 7 on the bottom surface of go-between 16, and integrated into one piece has many spliced poles 8 on the clamp ring 7, has offered the connecting hole that supplies spliced pole 8 to peg graft on the sealed lid 13, has offered a plurality of first holes of stepping down that supply spliced pole 8 to insert on the go-between 16, has all offered a plurality of second holes of stepping down that supply spliced pole 8 to insert on first filter screen 2 and the second filter screen 3. The connecting column 8 penetrates through the first yielding hole and the second yielding hole and penetrates out of the connecting hole of the sealing cover 13, so that the sealing cover 13 and the clamping ring 7 clamp the connecting ring 16, the first filter screen 2 and the second filter screen 3 for sealing.
Referring to fig. 3 and 4, the liquid inlet pipe 4 and the connecting pipe 6 are both disposed on the sealing cover 13, and are communicated with the first filter screen 2 in the connecting portion 11 through the sealing cover 13. The liquid outlet pipe 5 is communicated with the bottom end of the collecting part 12.
Referring to fig. 3 and 4, a partition 10 is provided between the collecting portion 12 and the connecting portion 11, and the partition 10 is an artificially divided area, and in actual use, a separation line is printed between the connecting portion 11 and the collecting portion 12, and the connecting portion 11 and the collecting portion 12 are fused or heat-sealed along the separation line. The collection portion 12 can be closed by heat sealing so that the collection portion 12 is independent of the connection portion 11, facilitating aseptic collection of the residue obtained by filtration. The collecting part 12 can be sealed through fusing, and meanwhile, the collecting part 12 is disconnected from the connecting part 11, so that the collecting part 12 can be stored independently, and obtained residues can be stored or processed independently conveniently. Meanwhile, the collecting part 12 is separated from the connecting part 11, so that the sealing cover 13 can be conveniently recycled after cell filtration is completed.
Referring to fig. 3 and 5, the connecting portion 11 is provided with a mounting hole 15, the mounting hole 15 is internally provided with an agitating assembly 9, the agitating assembly 9 comprises an agitating block 92, a rotating shaft 91 and a mounting seat 93, the mounting seat 93 is mounted on the connecting portion 11 through the mounting hole 15, the rotating shaft 91 is arranged in the mounting seat 93 and extends into the connecting portion 11 through the mounting seat 93, the agitating block 92 is fixed at one end of the rotating shaft 91 in the connecting portion 11, and a handle is arranged at one end of the rotating shaft 91 outside the connecting portion 11. Through rotating the handle, can rotate the stirring piece 92 in the connecting portion 11, stirring piece 92 striking second filter screen 3 promotes the mobility of the interior mixture of second filter screen 3, reduces the condition that appears second filter screen 3 to be stopped up, promotes the filtration efficiency of second filter screen 3 to the mixture.
Referring to fig. 3 and 5, the mounting base 93 includes a first connecting plate 931, a second connecting plate 932, a top block 933 and a connecting sleeve 934, the top block 933 is fixed on the first connecting plate 931, the connecting sleeve 934 is disposed on the second connecting plate 932, one end of the top block 933 away from the first connecting plate 931 is integrally formed with a guide block 935, and the diameter of the guide block 935 is smaller than the diameter of the top block 933. The first connecting plate 931 is arranged in the connecting portion 11, the guide block 935 is inserted into the mounting hole 15, the inner wall of the mounting hole 15 is attached to the side wall of the guide block 935, the top block 933 cannot penetrate out of the connecting portion 11, the second connecting portion 11 is arranged outside the connecting portion 11, the connecting sleeve 934 is sleeved on the top block 933, the connecting portion 11 is clamped by the connecting sleeve 934 and the top block 933, and a gap between the connecting sleeve 934 and the top block 933 is sealed by the connecting portion 11.
The implementation principle of the embodiment 2 of the invention is as follows: the mount 93 is installed in the connection portion 11, and the rotation shaft 91 and the agitating block 92 are installed. The second filter screen 3 and the first filter screen 2 are sequentially placed in the connecting part 11, the first filter screen 2 and the second filter screen 3 are fixed in the connecting part 11 through the sealing cover 13 and the clamping ring 7, and meanwhile, the bag body 1 is sealed by means of the sealing cover 13. The mixture is introduced into the first filter screen 2 through the liquid inlet pipe 4, and the mixture is filtered. The handle is rotated so that the stirring block 92 collides with the second filter screen 3, thereby improving the filtering efficiency of the second filter screen 3. After the mixture is filtered, the collecting part 12 is heat-sealed along the separating part 10, and the opening of the collecting part 12 is closed, so that the collecting part 12 is separated from the connecting part 11, and the remainder is convenient for subsequent use. The mounting seat 93 and the sealing cover 13 on the connecting portion 11 can be detached, cleaned, and mounted on the connecting portion 11 of the next bag body 1, and can be reused.
The above embodiments are not intended to limit the scope of the present invention, so: all equivalent changes in structure, shape and principle of the invention should be covered in the scope of protection of the invention.
Claims (8)
1. A cell filter bag, characterized in that: including the bag body (1), be equipped with a plurality of layers of filter screen in the bag body (1), a plurality of layers the hole of filter screen is radial reduce gradually in the bag body (1), be equipped with feed liquor pipe (4) on the bag body (1), feed liquor pipe (4) set up filter screen aperture great one side, be equipped with drain pipe (5) on the bag body (1), drain pipe (5) set up filter screen aperture less one side.
2. The cell filter bag of claim 1, wherein: the filter screen is connected with the bag body (1) through a high-frequency heat sealing process.
3. The cell filter bag of claim 1, wherein: the bag body (1) is communicated with a connecting pipe (6).
4. The cell filter bag of claim 1, wherein: the bag body (1) comprises a connecting portion (11) and a collecting portion (12), the collecting portion (12) is communicated with the connecting portion (11), the filter screen is arranged in the connecting portion (11), and a separation portion (10) is arranged between the connecting portion (11) and the collecting portion (12).
5. The cell filter bag of claim 4 wherein: the bag body (1) is provided with a mounting hole (15), and an agitating assembly (9) is arranged in the mounting hole (15).
6. The cell filter bag of claim 5 wherein: stirring subassembly (9) are including stirring piece (92), pivot (91) and mount pad (93), mount pad (93) set up in mounting hole (15), pivot (91) set up in mount pad (93), pivot (91) one end is in connecting portion (11), the other end is in outside connecting portion (11), stirring piece (92) set up in one end of pivot (91) in connecting portion (11).
7. The cell filter bag of claim 6 wherein: the mounting seat (93) comprises a first connecting plate (931), a second connecting plate (932), a top block (933) and a connecting sleeve (934), wherein the top block (933) is arranged on the first connecting plate (931), the connecting sleeve (934) is arranged on the second connecting plate (932), and a guide block (935) is arranged at one end, far away from the first connecting plate (931), of the top block (933).
8. The cell filter bag of claim 4 wherein: the bag body (1) further comprises a sealing cover (13), the sealing cover (13) is arranged on one side of the connecting part (11) away from the collecting part (12), a connecting ring (16) is arranged on the outer wall of the connecting part (11), one side of the connecting ring (16) away from the sealing cover (13) is provided with a clamping ring (7), a plurality of connecting columns (8) are arranged on the clamping ring (7), connecting holes for inserting the connecting columns (8) are formed in the sealing cover (13), a plurality of first yielding holes for inserting the connecting columns (8) are formed in the connecting ring (16), and a plurality of second yielding holes for inserting the connecting columns (8) are formed in the filter screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311052353.1A CN117025367A (en) | 2023-08-21 | 2023-08-21 | Cell filter bag |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311052353.1A CN117025367A (en) | 2023-08-21 | 2023-08-21 | Cell filter bag |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117025367A true CN117025367A (en) | 2023-11-10 |
Family
ID=88622516
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311052353.1A Pending CN117025367A (en) | 2023-08-21 | 2023-08-21 | Cell filter bag |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117025367A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE20211372U1 (en) * | 2002-07-27 | 2003-12-11 | Hjs Fahrzeugtechnik Gmbh & Co. | Exhaust gas particle filter for removing pollutants from a diesel engine exhaust gases comprises a filter body having filter pockets formed from a filter material strip |
| JP2005138064A (en) * | 2003-11-10 | 2005-06-02 | Mitaka Kogyosho:Kk | Filter and filter bag used for the same |
| CN107438663A (en) * | 2015-04-20 | 2017-12-05 | 通用电气医疗集团生物科学公司 | Inactivation of virus |
| CN209669220U (en) * | 2019-01-16 | 2019-11-22 | 甘肃中医药大学 | A kind of filter separator for mesenchymal stem cells |
| CN111214879A (en) * | 2019-11-01 | 2020-06-02 | 上海蓓蕊医疗科技有限公司 | Cell negative pressure filtering device and use method thereof |
| CN215961397U (en) * | 2021-08-13 | 2022-03-08 | 东南大学附属中大医院 | Novel filtration formula drainage bag |
| CN218811806U (en) * | 2022-11-23 | 2023-04-07 | 上海鸿声药业有限公司 | Cell culture bag |
-
2023
- 2023-08-21 CN CN202311052353.1A patent/CN117025367A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE20211372U1 (en) * | 2002-07-27 | 2003-12-11 | Hjs Fahrzeugtechnik Gmbh & Co. | Exhaust gas particle filter for removing pollutants from a diesel engine exhaust gases comprises a filter body having filter pockets formed from a filter material strip |
| JP2005138064A (en) * | 2003-11-10 | 2005-06-02 | Mitaka Kogyosho:Kk | Filter and filter bag used for the same |
| CN107438663A (en) * | 2015-04-20 | 2017-12-05 | 通用电气医疗集团生物科学公司 | Inactivation of virus |
| CN209669220U (en) * | 2019-01-16 | 2019-11-22 | 甘肃中医药大学 | A kind of filter separator for mesenchymal stem cells |
| CN111214879A (en) * | 2019-11-01 | 2020-06-02 | 上海蓓蕊医疗科技有限公司 | Cell negative pressure filtering device and use method thereof |
| CN215961397U (en) * | 2021-08-13 | 2022-03-08 | 东南大学附属中大医院 | Novel filtration formula drainage bag |
| CN218811806U (en) * | 2022-11-23 | 2023-04-07 | 上海鸿声药业有限公司 | Cell culture bag |
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