CN206273827U - A kind of filter separator for mesenchymal stem cells - Google Patents
A kind of filter separator for mesenchymal stem cells Download PDFInfo
- Publication number
- CN206273827U CN206273827U CN201621392154.0U CN201621392154U CN206273827U CN 206273827 U CN206273827 U CN 206273827U CN 201621392154 U CN201621392154 U CN 201621392154U CN 206273827 U CN206273827 U CN 206273827U
- Authority
- CN
- China
- Prior art keywords
- separating pipe
- pipe
- screen cloth
- air inlet
- stem cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model is related to a kind of filter separator for mesenchymal stem cells, including the upper separating pipe and lower separating pipe that are connected through a screw thread, wherein internal thread is provided with the internal perisporium of upper separating pipe bottom, lower separating pipe upper end periphery wall is provided with and is engaged external screw thread with internal thread, upper separating pipe and lower separating pipe joint are fixed with screen cloth, closure is housed at the upper port of upper separating pipe, feed tube and air inlet pipe are fixed with closure, air inlet pipe is connected with aseptic source of the gas, air inlet pipe is provided with the first magnetic valve, feed tube is provided with the second magnetic valve, side wall of the lower separating pipe upper-end part of driving below screen cloth is provided with voltage stabilizing mouthful and separates equalizing reservoir inner and outer air pressure when preparing device works for stem cell, sterilizing filter is installed at voltage stabilizing mouthful, lower separation bottom of the tube is provided with outlet, bottom is installed in outlet.The utility model substantially increases separative efficiency, and whole operation process time is short, reduces the chance that pollution occurs, and improves mescenchymal stem cell survival rate.
Description
Technical field
The utility model is related to a kind of cell separation apparatus, and in particular to a kind of filter separator for mesenchymal stem cells.
Background technology
Current mescenchymal stem cell is separated typically using screen cloth by rocking, stirring and shake come after reaching cell and digesting
The separate purpose of tissue block, its low separation efficiency, stem cell harvest yield is few, wastes time and energy, and exposure operation for a long time, does
Cell contamination chance is greatly increased.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of filter separator for mesenchymal stem cells, and it separates effect
Rate is high, time saving and energy saving, reduces stem cell pollution probability.
To achieve the above object, the technical scheme that the utility model is taken is：
A kind of filter separator for mesenchymal stem cells, including the upper separating pipe and lower separating pipe being connected through a screw thread, wherein
Internal thread is provided with the internal perisporium of upper separating pipe bottom, lower separating pipe upper end periphery wall is provided with and be engaged outer with internal thread
Screw thread, upper separating pipe and lower separating pipe joint are fixed with screen cloth, closure are housed, on closure at the upper port of upper separating pipe
Feed tube and air inlet pipe are fixed with, air inlet pipe is connected with aseptic source of the gas, and air inlet pipe is provided with the first magnetic valve, and feed tube is provided with
Second magnetic valve, side wall of the lower separating pipe upper-end part of driving below screen cloth is provided with voltage stabilizing mouthful and separates preparing device work for stem cell
Equalizing reservoir inner and outer air pressure when making, sterilizing filter is provided with voltage stabilizing mouthful, and lower separation bottom of the tube is provided with outlet, is installed in outlet
There is bottom.
Annular slot is offered on the lower separating pipe upper end internal perisporium, the screen cloth is stuck in annular slot.
The specification of the screen cloth is 100 mesh -300 mesh.
The screen cloth is made of stainless steel.
Upper separating pipe of the present utility model and lower separating pipe are connected through a screw thread, and it is convenient to dismantle, be easy to upper separating pipe, under
Handling, washing and the sterilizing of separating pipe and screen cloth, are easy to stem cell collection, by being done for extracting mesenchyma after enzymolysis, digestion
The mixture of the primary cell of cell and tissue by feed tube into falling on screen cloth in upper separating pipe, then by air inlet pipe
With inflating pressure in the upward separating pipe of aseptic source of the gas so that cell is acted on the mixture of tissue in sole mass and ambient pressure
Under can fast filtering separate, substantially increase separative efficiency, whole operation process time is short, reduce pollution occur chance,
Improve mescenchymal stem cell survival rate.
Brief description of the drawings
Fig. 1 is the structural representation of the utility model embodiment.
Fig. 2 is a-quadrant enlarged diagram in Fig. 1.
Specific embodiment
The utility model is described in further detail below in conjunction with the accompanying drawings.
As shown in Figure 1 and Figure 2, a kind of filter separator for mesenchymal stem cells, including the upper separating pipe 1 being connected through a screw thread
With lower separating pipe 2, wherein being provided with internal thread on the internal perisporium of upper separating pipe bottom, lower separating pipe upper end periphery wall is provided with
The external screw thread being engaged with internal thread, so facilitates the dismounting of whole separator, and upper separating pipe 1 and the lower joint of separating pipe 2 are solid
Surely there is screen cloth 3, the specification of screen cloth is 100 mesh -300 mesh, and screen cloth is made of stainless steel, and it can be by the slightly larger group of diameter
Knit and filter out and mescenchymal stem cell passes through, after completion stem cell is separated by filtration, upper separating pipe 1 is unloaded from lower separating pipe 2
Under, remove screen cloth just can be collected to stem cell, very convenient.Closure 4, closure are housed at the upper port of upper separating pipe
4 can be by being threadably mounted in upper separating pipe, and cleaning is easy in so convenient handling.Feed tube 5 is fixed with closure 4 and is entered
Tracheae 6, air inlet pipe 6 is connected with aseptic source of the gas, and air inlet pipe 6 is provided with the first magnetic valve 7, and feed tube 5 is provided with the second magnetic valve
8, side wall of the lower upper-end part of driving of separating pipe 2 below screen cloth is provided with voltage stabilizing mouthful 9, can be connected with what is be bent upwards in voltage stabilizing mouthful 9
Voltage-stabiliser tube 13, voltage stabilizing mouthful 9 is used for stem cell and separates equalizing reservoir inner and outer air pressure when preparing device works, and is provided with voltage-stabiliser tube 13 and removed
Bacterium filter 10, lower separation bottom of the tube forms outlet 11, and bottom 12 is provided with outlet.
Annular slot is offered on the upper end internal perisporium of lower separating pipe 2, screen cloth 3 is stuck in annular slot, conveniently picks and places.
When the utility model is specifically used, be first turned on the second magnetic valve 8 so that by after enzymolysis, digestion for extracting
The mixture of the primary cell of mescenchymal stem cell and tissue, into falling on screen cloth 3 in upper separating pipe, is mixed by feed tube 5
The liquid level of thing will be less than the outlet of air inlet pipe 6, be then shut off the second magnetic valve 8, open the first magnetic valve 7, and aseptic source of the gas passes through
Inflating pressure in the upward separating pipe of air inlet pipe 6 so that cell is with the mixture organized under sole mass and ambient pressure effect
Can fast filtering separate by screen cloth 3, it is to be filtered has separated after, upper separating pipe 1 is unloaded from lower separating pipe 2, taking-up screen cloth 3
Filtrate is washed, mescenchymal stem cell falls into lower separation bottom of the tube, the collection mescenchymal stem cell of bottom 12 can be opened and passed
It is commissioned to train and supports or be further purified using other means.
Claims (4)
1. a kind of filter separator for mesenchymal stem cells, it is characterised in that：Including the upper separating pipe being connected through a screw thread and lower point
From pipe, wherein being provided with internal thread on the internal perisporium of upper separating pipe bottom, lower separating pipe upper end periphery wall is provided with and interior spiral shell
Line is engaged external screw thread, and upper separating pipe and lower separating pipe joint are fixed with screen cloth, equipped with sealing at the upper port of upper separating pipe
Lid, is fixed with feed tube and air inlet pipe on closure, air inlet pipe is connected with aseptic source of the gas, and air inlet pipe is provided with the first magnetic valve,
Feed tube is provided with the second magnetic valve, and side wall of the lower separating pipe upper-end part of driving below screen cloth is provided with voltage stabilizing mouthful for stem cell
Equalizing reservoir inner and outer air pressure when preparing device works is separated, sterilizing filter is installed at voltage stabilizing mouthful, lower separation bottom of the tube is provided with out
Mouthful, bottom is installed in outlet.
2. filter separator for mesenchymal stem cells according to claim 1, it is characterised in that：The lower separating pipe upper end
Annular slot is offered on internal perisporium, the screen cloth is stuck in annular slot.
3. filter separator for mesenchymal stem cells according to claim 2, it is characterised in that：The specification of the screen cloth is
100 mesh -300 mesh.
4. filter separator for mesenchymal stem cells according to claim 3, it is characterised in that：The screen cloth uses stainless steel
Material is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621392154.0U CN206273827U (en) | 2016-12-19 | 2016-12-19 | A kind of filter separator for mesenchymal stem cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201621392154.0U CN206273827U (en) | 2016-12-19 | 2016-12-19 | A kind of filter separator for mesenchymal stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206273827U true CN206273827U (en) | 2017-06-23 |
Family
ID=59063192
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201621392154.0U Expired - Fee Related CN206273827U (en) | 2016-12-19 | 2016-12-19 | A kind of filter separator for mesenchymal stem cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206273827U (en) |
-
2016
- 2016-12-19 CN CN201621392154.0U patent/CN206273827U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170623 Termination date: 20201219 |