CN208857270U - Stem cell extraction device - Google Patents
Stem cell extraction device Download PDFInfo
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- CN208857270U CN208857270U CN201821465876.3U CN201821465876U CN208857270U CN 208857270 U CN208857270 U CN 208857270U CN 201821465876 U CN201821465876 U CN 201821465876U CN 208857270 U CN208857270 U CN 208857270U
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- stem cell
- wall
- outlet pipe
- blood
- chamber
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Abstract
The utility model discloses Stem cell extraction devices, including fixed frame, the middle position of the fixed frame top exterior walls is provided with washer box, and the top side outer wall of washer box is provided with salt water inlet pipe, the top side outer wall of the washer box is provided with blood inlet pipe, the bottom side inner wall of the fixed frame is provided with fixing seat, and the top exterior walls of fixing seat are provided with separator box, the bottom outer wall of the separator box is provided with blood plasma outlet pipe, stem cell outlet pipe and red blood cell outlet pipe, and blood plasma outlet pipe is internally provided with second one-way valve, the stem cell outlet pipe is internally provided with third check valve.Blood is passed through brine by the utility model, remove blood coagulation particle, reduce the stickiness of blood plasma, blood is centrifuged using ultracentrifuge, filter mixed cell mass, blood coagulation particle and pollutant in stem cell, accelerate separating rate, avoids stem cell contaminated, keep the activity of stem cell.
Description
Technical field
The utility model relates to cell extraction techniques field more particularly to Stem cell extraction devices.
Background technique
Stem cell refers to the initial cell with self-renewing and multi-lineage potential, can be divided under given conditions
For one or more composition tissues or the cell of organ.Self-renewing refers to that stem cell can remain dry by cell division
The size of cell colony;Multidirectional Differentiation refers to that stem cell has plasticity.
Currently, people often extract various stem cells to carry out since stem cell has plastic function
Pairing is usually used in treating disease in the blood system and histoorgan transplanting etc. to maintain each histiocytic function normality of human body.
Stem cell is during the extraction process, it is often necessary to arrive a kind of stem cell separator, the stem cell in blood plasma is separated and is mentioned again
The stem cell separation and Extraction pipe for taking, however using in the market is all the separating pipe of syringe form, the consumption of separation and Extraction mostly
Duration is unfavorable for cell and keeps activity, and it is contaminated to easily cause stem cell to contact when stem cell separation and Extraction with air,
Meanwhile blood plasma itself may have pollutant, it is not pure enough to extract stem cell, therefore, needs design stem cell and extracts
Device solves the above problems.
Summary of the invention
Purpose of the utility model is to solve disadvantages existing in the prior art, and the stem cell proposed extracts dress
It sets.
To achieve the goals above, the utility model adopts the technical scheme that
The middle position of Stem cell extraction device, including fixed frame, the fixed frame top exterior walls is provided with washer box, and
The top side outer wall of washer box is provided with salt water inlet pipe, and the top side outer wall of the washer box is provided with blood inlet pipe, institute
The bottom side inner wall for stating fixed frame is provided with fixing seat, and the top exterior walls of fixing seat are provided with separator box, the separator box
Bottom outer wall be provided with blood plasma outlet pipe, stem cell outlet pipe and red blood cell outlet pipe, and blood plasma outlet pipe to be internally provided with second single
To valve, the stem cell outlet pipe is internally provided with third check valve, and the red blood cell outlet pipe is internally provided with the 4th unidirectionally
Valve, the side outer wall of the separator box are welded with separating liquid inlet pipe, and the middle position of the fixed frame bottom interior wall is provided with super
Speed centrifugation motor, and the inside of separator box is arranged in the rotor of ultracentrifugation motor, the bottom outer wall of the fixed frame is provided with
Discharging chamber, and the bottom interior wall of discharging chamber is provided with partition, discharging chamber is separated into blood plasma storage chamber by the partition, stem cell stores up
Deposit chamber and red blood cells storage chamber.
The middle position of the washer box bottom outer wall is provided with guide as a further solution of the present invention,
Pipe, and passage is internally provided with the first check valve, the inside of separator box is arranged in passage.
Blood plasma storage chamber is arranged in the bottom outer wall of the blood plasma outlet pipe as a further solution of the present invention,
Inside, and the inside of stem cell outlet pipe is arranged in stem cell storage chamber, the inside of red blood cell outlet pipe is arranged in red blood cells storage chamber.
The bottom outer wall of the discharging chamber is provided with stem cell and extracts chamber as a further solution of the present invention,
And stem cell extracts the underface that chamber is located at stem cell storage chamber, the middle position that stem cell extracts chamber bottom outer wall is provided with super
Filter semi-permeable membrane.
The middle position that the stem cell extracts chamber bottom outer wall as a further solution of the present invention, is provided with
Perforation, and the inner wall perforated is provided with extraction tube.
The bottom interior wall of the extraction tube is provided with pull plate, and pull as a further solution of the present invention,
The top exterior walls of plate are provided with rubber piston, and the outer wall of rubber piston and the inner wall of extraction tube are seamlessly connected.
The bottom side inner wall of the extraction tube is provided with limited block as a further solution of the present invention, and
The side outer wall of extraction tube is provided with discharge port, and the inner wall of discharge port is provided with stem cell discharge nozzle.
The utility model has the following beneficial effects:
1. blood is first passed through physiological saline by washer box, salt water inlet pipe, separator box and the ultracentrifugation motor of setting
Washing, to remove blood coagulation particle, reduces the stickiness of blood plasma, then enters in separator box through the first check valve, with separating liquid
Mixing, is centrifuged using ultracentrifuge, is separated to blood, blood is made to be divided into plasma layer, layer and red blood cell
Layer, convenient for the subsequent extraction to stem cell;
2. by the ultrafiltration semi-permeable membrane of setting, on the one hand to being mixed into cell mass in stem cell and blood coagulation particle is filtered,
Accelerate separating rate, on the other hand by contaminant removal mixed in stem cell, avoids the stem cell extracted contaminated, keep dry
The activity of cell;
3. by setting pull plate, rubber piston and limited block, since the top exterior walls of limited block are to stem cell discharge nozzle
Bottom outer wall between length and rubber piston it is of same size, it is ensured that the stem cell that pull plate is released can be complete
It is come out from stem cell discharge nozzle, more avoids pull plate and fall off and cause stem cell to contact with air and contaminated.
Detailed description of the invention
Fig. 1 be the utility model proposes Stem cell extraction device structural schematic diagram;
Fig. 2 be the utility model proposes Stem cell extraction device ultrafiltration semi-permeable membrane and extraction tube structural schematic diagram;
Fig. 3 be the utility model proposes Stem cell extraction device separator box structural schematic diagram.
In figure: 1 fixed frame, 2 washer boxs, 3 salt water inlet pipes, 4 blood inlet pipes, 5 passages, 6 first check valves, 7 separator boxs,
8 ultracentrifugation motors, 9 blood plasma outlet pipes, 10 second one-way valves, 11 stem cell outlet pipes, 12 red blood cell outlet pipes, 13 discharging chambers, 14 blood
Starch storage chamber, 15 stem cell storage chambers, 16 red blood cells storage chambers, 17 stem cells extract chamber, 18 ultrafiltration semi-permeable membranes, 19 extraction tubes,
20 rubber pistons, 21 pull plates, 22 limited blocks, 23 stem cell discharge nozzles.
Specific embodiment
The following will be combined with the drawings in the embodiments of the present invention, carries out the technical scheme in the embodiment of the utility model
Clearly and completely describe, it is clear that the described embodiments are only a part of the embodiments of the utility model, rather than whole
Embodiment.
Referring to Fig.1-3, the middle position of Stem cell extraction device, including fixed frame 1,1 top exterior walls of fixed frame passes through spiral shell
It tethers and is connected to washer box 2, and the top side outer wall of washer box 2 is welded with salt water inlet pipe 3, blood is washed by physiology salt
It washs, to remove blood coagulation particle, reduces the stickiness of blood, the top side outer wall of washer box 2 is welded with blood inlet pipe 4, fixed
The bottom side inner wall of frame 1 is welded with fixing seat, and separator box 7, separator box 7 has been bolted in the top exterior walls of fixing seat
Bottom outer wall be welded with blood plasma outlet pipe 9, stem cell outlet pipe 11 and red blood cell outlet pipe 12, and the inside of blood plasma outlet pipe 9 passes through spiral shell
It tethers and is connected to second one-way valve 10, third check valve, red blood cell outlet pipe 12 has been bolted in the inside of stem cell outlet pipe 11
Inside the 4th check valve has been bolted, the side outer wall of separator box 7 is welded with separating liquid inlet pipe, 1 bottom of fixed frame
Ultracentrifugation motor 8 has been bolted in the middle position of inner wall, and the rotor plug of ultracentrifugation motor 8 is in separator box 7
Inside, blood plasma is mixed with separating liquid, is centrifuged using ultracentrifuge 8, blood is separated, blood plasma is made to be divided into blood
Pulp layer, layer and red blood cell layer, the bottom outer wall of fixed frame 1 have been bolted discharging chamber 13, and discharging chamber 13
Bottom interior wall is welded with partition, and discharging chamber 13 is separated into blood plasma storage chamber 14, stem cell storage chamber 15 and red blood cell and stored up by partition
Deposit chamber 16.
In the utility model, the middle position of 2 bottom outer wall of washer box is welded with passage 5, and the inside of passage 5 is logical
It crosses and has been bolted the first check valve 6, passage 5 is plugged on the inside of separator box 7, and the bottom outer wall of blood plasma outlet pipe 9 is plugged on
The inside of blood plasma storage chamber 14, and stem cell storage chamber 15 is plugged on the inside of stem cell outlet pipe 11, red blood cells storage chamber 16 is inserted
It connects in the inside of red blood cell outlet pipe 12, the bottom outer wall of discharging chamber 13 has been bolted stem cell and has extracted chamber 17, and dry thin
Born of the same parents extract the underface that chamber 17 is located at stem cell storage chamber 15, and the middle position that stem cell extracts 17 bottom outer wall of chamber is adhesive with super
Semi-permeable membrane 18 is filtered, filters in stem cell and is mixed into cell mass, blood coagulation particle and pollutant, avoids the stem cell extracted contaminated, protects
The activity of stem cell is held, the middle position that stem cell extracts 17 bottom outer wall of chamber is provided with perforation, and the inner wall perforated is welded with and mentions
Pipe 19 is taken, the bottom interior wall of extraction tube 19 is plugged with pull plate 21, and the top exterior walls of pull plate 21 are socketed with rubber piston 20,
The outer wall of rubber piston 20 and the inner wall of extraction tube 19 are seamlessly connected, and the bottom side inner wall of extraction tube 19 is welded with limited block
22, the stem cell for guaranteeing that pull plate 21 is released can come out from stem cell discharge nozzle 23 completely, avoid pull plate 21 and fall off
Stem cell is caused to contact with air and contaminated, and the side outer wall of extraction tube 19 is provided with discharge port, the inner wall welding of discharge port
There is a stem cell discharge nozzle 23, the top exterior walls of limited block 22 to the length and rubber between the bottom outer wall of stem cell discharge nozzle 23
Piston 20 it is of same size.
Working principle: in use, user passes through salt water inlet pipe 3 to saline injection in washer box 2, and pass through blood
Inlet pipe 4 is to blood is injected in washer box 2, to remove blood coagulation particle, reduces the stickiness of blood, user's starting passes through separating liquid
Inlet pipe restarts ultracentrifugation motor 8 to separating liquid is injected in separator box 7, opens the first check valve 6, and blood enters separator box 7
It is interior, after being centrifuged standing, plasma layer, layer and red blood cell layer are divided into separator box 7 from top to bottom, user opens
Second one-way valve 10 is imported blood plasma in blood plasma storage chamber 14 by blood plasma outlet pipe 9, and user opens third check valve, passes through
Stem cell outlet pipe 11 imports stem cell in stem cell storage chamber 15, and user opens the 4th check valve, passes through red blood cell outlet pipe
12 import red blood cell in red blood cells storage chamber 16, and user twitches pull plate 21, and rubber piston 20 is moved to limited block 22
On, so that stem cell is entered back into extraction tube 19 by the filtering and impurity removing matter of ultrafiltration semi-permeable membrane 18, from stem cell discharge nozzle 23
In extract.
The preferable specific embodiment of the above, only the utility model, but the protection scope of the utility model is not
It is confined to this, anyone skilled in the art is within the technical scope disclosed by the utility model, practical according to this
Novel technical solution and its utility model design are subject to equivalent substitution or change, should all cover the protection model in the utility model
Within enclosing.
Claims (7)
1. Stem cell extraction device, including fixed frame (1), which is characterized in that the middle position of fixed frame (1) top exterior walls
It is provided with washer box (2), and the top side outer wall of washer box (2) is provided with salt water inlet pipe (3), the top of the washer box (2)
Portion side outer wall is provided with blood inlet pipe (4), and the bottom side inner wall of the fixed frame (1) is provided with fixing seat, and fixing seat
Top exterior walls be provided with separator box (7), the bottom outer wall of the separator box (7) is provided with blood plasma outlet pipe (9), stem cell outlet pipe
, (11) and red blood cell outlet pipe (12) and blood plasma outlet pipe (9) is internally provided with second one-way valve (10), the stem cell outlet pipe
(11) be internally provided with third check valve, the red blood cell outlet pipe (12) is internally provided with the 4th check valve, the separation
The side outer wall of case (7) is welded with separating liquid inlet pipe, and the middle position of fixed frame (1) bottom interior wall is provided with ultracentrifugation
Motor (8), and the rotor of ultracentrifugation motor (8) is arranged in the inside of separator box (7), the bottom outer wall of the fixed frame (1)
It is provided with discharging chamber (13), and the bottom interior wall of discharging chamber (13) is provided with partition, discharging chamber (13) are separated by the partition
Blood plasma storage chamber (14), stem cell storage chamber (15) and red blood cells storage chamber (16).
2. Stem cell extraction device according to claim 1, which is characterized in that in washer box (2) bottom outer wall
Meta position installs is equipped with passage (5), and passage (5) is internally provided with the first check valve (6), passage (5) setting point
Inside from case (7).
3. Stem cell extraction device according to claim 1, which is characterized in that the bottom outer wall of the blood plasma outlet pipe (9)
Inside in blood plasma storage chamber (14) is set, and stem cell storage chamber (15) setting is in the inside of stem cell outlet pipe (11), it is red thin
Born of the same parents' storage chamber (16) is arranged in the inside of red blood cell outlet pipe (12).
4. Stem cell extraction device according to claim 1, which is characterized in that the bottom outer wall of the discharging chamber (13) is set
It is equipped with stem cell and extracts chamber (17), and stem cell extracts the underface that chamber (17) are located at stem cell storage chamber (15), stem cell mentions
The middle position of chamber (17) bottom outer wall is taken to be provided with ultrafiltration semi-permeable membrane (18).
5. Stem cell extraction device according to claim 1, which is characterized in that the stem cell is extracted outside chamber (17) bottom
The middle position of wall is provided with perforation, and the inner wall perforated is provided with extraction tube (19).
6. Stem cell extraction device according to claim 5, which is characterized in that the bottom interior wall of the extraction tube (19) is set
Be equipped with pull plate (21), and the top exterior walls of pull plate (21) are provided with rubber piston (20), the outer wall of rubber piston (20) with
The inner wall of extraction tube (19) is seamlessly connected.
7. Stem cell extraction device according to claim 6, which is characterized in that in the bottom side of the extraction tube (19)
Wall is provided with limited block (22), and the side outer wall of extraction tube (19) is provided with discharge port, and the inner wall of discharge port is provided with dry thin
Born of the same parents' discharge nozzle (23).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821465876.3U CN208857270U (en) | 2018-09-07 | 2018-09-07 | Stem cell extraction device |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201821465876.3U CN208857270U (en) | 2018-09-07 | 2018-09-07 | Stem cell extraction device |
Publications (1)
Publication Number | Publication Date |
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CN208857270U true CN208857270U (en) | 2019-05-14 |
Family
ID=66417176
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
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CN201821465876.3U Expired - Fee Related CN208857270U (en) | 2018-09-07 | 2018-09-07 | Stem cell extraction device |
Country Status (1)
Country | Link |
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CN (1) | CN208857270U (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111575148A (en) * | 2020-04-27 | 2020-08-25 | 通用生物系统(安徽)有限公司 | Device and method for rapidly extracting DNA in batches |
-
2018
- 2018-09-07 CN CN201821465876.3U patent/CN208857270U/en not_active Expired - Fee Related
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111575148A (en) * | 2020-04-27 | 2020-08-25 | 通用生物系统(安徽)有限公司 | Device and method for rapidly extracting DNA in batches |
CN111575148B (en) * | 2020-04-27 | 2023-05-16 | 通用生物(安徽)股份有限公司 | Device and method for rapidly extracting DNA in batches |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20190514 |