KR20190019001A - Apparatus for separating adipocyte and method of separating adipocyte using the same - Google Patents

Abstract

The present invention provides an apparatus for separating adipocytes and a method of separating adipocytes using the same, which comprises a flexible container, a porous filter membrane, an injection port into which untreated fat tissue is injected, and a discharge port for discharging impurities. The present invention can efficiently separate adipocytes at high purity without damaging the same.

Description

FIELD OF THE INVENTION [0001] The present invention relates to a method for separating adipocytes using a fat-splitting device and a fat-

The present invention relates to a method for separating adipocytes using a device for fat ripening and a device for fat ripening. More particularly, the present invention relates to a fat splitting apparatus using a flexible container and a fat cell separation method using the fat splitting apparatus.

Fat transplantation to the human body is a cosmetic and therapeutic procedure commonly used. This includes, for example, breast / hip enlargement, wrinkle removal, and scar repair.

The aspiration of fat from the human body can be achieved by sound pressure liposuction, ultrasonic liposuction, or the like. At this time, a wetting solution containing a local anesthetic, a vasoconstrictor, and the like is injected into the fat. The function of the wet solution is to expand the subcutaneous fat layer to make the fat fall off well, to cause the blood vessels to be pressed and contracted to reduce the bleeding, and to be absorbed by the body to replenish body fluids.

In addition to adipocytes, the aspirated fat may include impurities such as wetting solution, blood vessels / blood components, and free fat.

If the transplanted adipose tissue contains a large amount of liquid, the volume of the transplanted adipose tissue may be reduced because the liquid is absorbed into the body of the transplanted site. In addition, if the adipose tissue contains blood cells such as red blood cells, white blood cells, lipids, etc., it may induce an inflammatory reaction in the transplanted area.

For this reason, it is necessary to isolate adipocytes at high purity in order to transplant adipocytes into the human body.

For example, it is possible to separate adipocytes by centrifugation or high-pressure filtration, which is disclosed in Korean Patent Laid-Open Publication No. 10-2006-0025180. However, in the above procedure, damage of adipocytes is caused by physical external force The problem needs to be solved.

Korean Patent Publication No. 10-2006-0025180 (Mar. 20, 2006)

An object of the present invention is to provide a high-efficiency fat ripening apparatus.

An object of the present invention is to provide a highly efficient method for separating adipocytes.

1. Flexible containers; A porous filter disposed in the flexible container and defining a filtration space; An injection port communicating with the filtering space from one end of the flexible container to inject untreated fat tissue; And an outlet disposed at the other end of the flexible container for discharging impurities separated from the untreated adipose tissue to the outside of the filtering space.

2. The apparatus for fat splitting as in 1 above, wherein said porous filter membrane is a porous filter bag having a bag shape.

3. The apparatus of claim 2, wherein the injection port is fused together with the one end of the flexible container and the top of the porous filter bag.

4. The apparatus for fat splitting as in 3 above, wherein the porous filter bag has a closed curved shape closed by the fused portion.

5. The apparatus of claim 4, wherein the porous filter bag is arranged to be floating in the space in the flexible container.

6. The apparatus for fat splitting according to 1 above, wherein the space inside the flexible container excluding the filtering space is defined as an impurity collection space.

7. The apparatus for fat splitting as set forth in claim 1, further comprising a cleaning liquid injection port communicated with said foliage space.

8. The apparatus of claim 6, further comprising a cleaning liquid injection port communicated with the impurity collection space.

9. The apparatus of claim 1, further comprising a collection port communicating with the filtering space from the one end of the flexible container to collect the adipose tissue or fat cells from which the impurities have been removed from the untreated adipose tissue.

10. The apparatus for fat splitting as in 1 above, wherein said outlet is provided in plurality.

11. The apparatus of claim 1, further comprising a sound pressure pump connected to said outlet.

12. The apparatus of claim 1, wherein the porous filter membrane extends from the one end to the other end across the inner space of the flexible container.

13. The flexible container according to claim 12, wherein the inner space of the flexible container is divided into upper and lower portions by the porous filter membrane, the upper portion communicates with the injection port to define the filtering space, and the lower portion communicates with the outlet And defines the impurity collection space.

14. A process for producing a flexible filter, comprising: a flexible container; a porous filter membrane disposed in the flexible container to define a filtration space; an inlet and a recovery port communicating with the filtering space from one end of the flexible container; and an outlet disposed at the other end of the flexible container Preparing a fat ripening device containing the fat ripening device; Injecting untreated adipose tissue through the injection port into the foliage space; Collecting impurities from the untreated fat tissue into the impurity collection space of the flexible container by discharging the impurities out of the filtration space; And removing the impurities through the outlet.

15. The method of claim 14, wherein removing the impurities through the outlet comprises discharging the impurities by applying a negative pressure to the impurity collection space through the outlet.

According to embodiments of the present invention, by separating adipocytes using a porous membrane, adipocytes can be isolated with high purity without damage by physical or mechanical action.

In addition, the porous filter bag may be arranged to float in the space in the flexible container or have a closed curved surface shape, thereby increasing the surface area of the filter bag used in the fat cell separation process, thereby separating a large amount of fat cells for a short time.

According to some embodiments, a sound pressure pump may be connected to the outlet to rapidly separate fat cells.

1 is a schematic plan view showing an apparatus for fat ripening according to exemplary embodiments;
2 is a side cross-sectional view of an apparatus for fat ripening according to exemplary embodiments.
Figures 3 and 4 are schematic plan views illustrating an apparatus for fat ripening according to some embodiments.
5 is a schematic side cross-sectional view of an apparatus for fat ripening according to some exemplary embodiments.

DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS The present invention will now be described in detail with reference to the accompanying drawings. While the following preferred embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes in form and details may be made without departing from the spirit and scope of the present invention as defined by the following claims. It will be obvious to those skilled in the art that such modifications and variations are within the scope of the appended claims.

According to embodiments of the present invention, there is provided an apparatus for fat splitting comprising a flexible container, a porous filter membrane, an inlet for injecting untreated fat tissue, and an outlet for discharging impurities, and a method for separating fat cells using the same.

As used herein, the term " untreated fat tissue " means an adipose tissue isolated from a human body, and may include adipose tissue, adipose tissue including blood cells, wetting solution, free fat and the like.

As used herein, the term " impurity " means an untreated adipose tissue excluding adipocytes, and may mean blood cells, wetting solution, free fat, and the like.

Fig. 1 is a schematic plan view showing an apparatus for fat ripening according to exemplary embodiments, and Fig. 2 is a side sectional view of an apparatus for fat rippling according to exemplary embodiments. Fig.

Hereinafter, with reference to Figs. 1 and 2, an apparatus for fat ripening according to exemplary embodiments will be described.

1 and 2, the fat burning apparatus 100 according to the exemplary embodiments includes a flexible container 110, a porous filter 130, a fountain space 150, an impurity collection space 170, And may include an inlet 210 and an outlet 250.

The injection port 210 may be connected to one end of the flexible container 110 and the filtering space 150. The untreated fat tissue may be injected into the filtering space 150 through the injection port 210 of the apparatus for fat ripening 100.

The flexible container 110 defines the outer shape of the apparatus for fat ripening 100. The flexible container 110 is a container which can be filled with untreated fat tissue. The flexible container 110 includes a filtering space 150 divided by the porous filtering membrane 130, And may include a space 170.

For example, the flexible container 110 may include materials having a material that is easily bendable and can recover its original shape when opened or folded. Non-limiting examples include ethylene vinyl acetate, polyvinyl chloride, nylon, polyethylene terephthalate, acrylonitrile butadiene styrene, polytetrafluoroethylene, There may be mentioned polytetrafluoroethylene, polycarbonate, polystyrene, polyurethane, polyethylene, polypropylene and the like. Preferably, it may be a material free from physical or chemical changes during sterilization processes such as autoclaving.

According to an exemplary embodiment, the porous filter membrane 130 can define the internal space of the flexible container 110 as the foliage space 150 and the impurity collection space 170. [

The porous filter membrane 130 may be formed of a material that can freely change its shape together with the flexible container 110 when the flexible container 110 is folded or folded and unfolded. And may be formed of the same material.

According to an exemplary embodiment, the porous filter membrane 130 may be attached to one surface of the interior of the flexible container 110 and integrated with the flexible container 110.

The porous membrane 130 may collect the adipocytes by selectively filtering the impurities from the untreated adipose tissue.

The porous membrane 130 may include a material capable of passing the impurities and filtering the adipocytes. When the untreated fat tissue is processed in the porous filter membrane 130, the impurities are passed to the impurity collection space 170, and the impregnated fat cells may remain in the foliage space 150.

According to one embodiment, the pore size of the porous filter membrane 130 may be from about 40 占 퐉 to about 200 占 퐉, and preferably from about 50 占 퐉 to about 150 占 퐉. The size of the hole may be appropriately selected in the above range so as to pass the impurities into the impurity collection space 170 and leave the adipocytes in the dung space 150. When the diameter of the hole exceeds about 200 μm, the fat cells may not be caught by the porous filter membrane 130. If the diameter of the hole is less than about 40 μm, the fat cells may be separated from the porous membrane 130 It can block the hole and prevent the separation of adipocytes.

For example, the porous filter membrane 130 may comprise a lipid adsorbent material or may be a lipid adsorbent material. The lipid adsorbing material may include a lipid adsorbing plastic such as polyethylene, nylon, rayon, cellulose nitrate, cellulose acetate and the like, or a resin material. Adipose cells smaller in size than the pores of the porous filter membrane 130 due to the lipid adsorption material are also adsorbed on the porous filter membrane 130 and remain in the filtration space 150 to increase the yield of the adipocyte separation process .

In some embodiments, porous membrane 130 may be, for example, a porous sanitary pouch having a pouch shape.

In one embodiment, the upper portion of the porous filter bag may be fused to one end of the flexible container 110, and may be fused with the injection port 210 and the recovery port 230. The outer and inner portions of the flexible container 110 are disconnected by the fusion, and the separation process of the fat cells can be performed without exposing the untreated fat tissue to the inside.

In exemplary embodiments, the porous filter bag may be in the form of a closed curved surface that is closed by the fused portion. If the porous filter bag has a closed curved surface shape closed by the fused portion, a larger area of the filter bag may be used for the fat cell separation process, thereby increasing the efficiency of the fat cell separation process. For example, the porous filter bag may substantially completely surround the untreated adipose tissue introduced through the injection port 210, thereby increasing the contact surface area with the untreated adipose tissue, can do.

As shown in FIG. 2, the porous filter bag may be arranged to be floating in the space in the flexible container 110. For example, the outer surface of the porous filter bag may be separated or spaced from the inner surface of the flexible container 110.

Accordingly, it is possible to prevent the untreated adipose tissue from remaining in the porous filter bag sufficiently while reducing separation efficiency due to adhesion with the flexible container 110 or integration. For example, the porous filter bag can be floated in the space within the flexible container 110 with a volume corresponding to about 40 to 70% of the flexible container 110.

According to exemplary embodiments, the impurities of the untreated adipose tissue may be discharged outside the foliage space 150 and collected in the impurity collection space 170.

The process of discharging the impurities out of the foliage space 150 can be performed by a physical and mechanical filtering process of a liquid and a solid mixture which are conventionally performed. For example, the injection port 210 of the fat dissolving apparatus 100 may be directed upward and the discharge port 250 may be directed downward so that the impurities may be released by gravity without any additional external force. For example, an external force such as shaking, externally applying pressure, or rotating the fat-dispersing device 100 containing untreated fat tissue may be applied to release the impurities out of the foliage space 150.

Compared to the separation process of the fat cells by centrifugation or high pressure filtration, when the impurities are discharged outside the feces space 150 by gravity or weak external force, the fat cells can be separated without physical and mechanical damage.

The outlet 250 may be disposed at the other end of the flexible container 110.

In the exemplary embodiments, the impurities may be removed through the outlet 250. The impurities having passed through the porous membrane 130 may be discharged to the outside of the apparatus for degassing 100 through the outlet 250 through the impurity collection space 170. Removal of the impurities may be performed by, for example, discharging the impurities by gravity with the discharge port 250 as a lower part, or by applying pressure to the apparatus 100 for oil fractionation.

In one embodiment, the recovery tool 230 may be provided in communication with one end of the flexible container 110 and the filtering space 150. It is possible to extract the adipose tissue or adipocyte from which the impurities have been removed from the untreated adipose tissue by being filtered by the porous filter membrane 130 through the collection port 230 and remaining in the filtering space 150.

When the adipocytes are extracted through the injection port 210, the adipocytes may be mixed with the untreated adipose tissue that may remain in the injection port 210. However, the adipocyte may be provided with a recovery port 230, The above problem can be solved by extracting.

3 is a schematic plan view showing an apparatus for fat ripening according to some embodiments. The description of the configuration described with reference to FIG. 1 or 2 of the configuration shown in FIG. 3 may be omitted.

Referring to FIG. 3, the apparatus for fat ripening 100 may further include a cleaning liquid injection port 290.

According to some embodiments, the cleaning fluid injection port 290 may communicate with the felling space 150 and may communicate with the felling space 150, for example, with the injection port 210 and the withdrawal port 230.

In some embodiments, the cleaning liquid injection port 290 may communicate with the impurity collection space 170 and may communicate with the impurity collection space 170, for example, with the outlet 250.

According to exemplary embodiments, the washing liquid can be injected into the apparatus for fat ripening 100 through the washing liquid injection port 290. For example, when the washing liquid injection port 290 is communicated with the filtering space 150, the washing liquid is injected into the impurity collection space 170 when the washing liquid injection port 290 is communicated with the impurity collection space 170 .

The washing liquid injected into the filtering space 150 or the impurity collecting space 170 can be cleaned from the untreated adipose tissue while passing through the filtering filter 150 and the impregnating space 150 and the impurity collecting space 170 have. By the washing, the impurities can be removed from the untreated fat tissue and contained in the washing liquid.

After the cleaning solution is injected, physical force such as flipping, shaking, or rubbing the fatring apparatus 100 may be applied to promote the washing of the untreated fat tissue and the fat cells by the washing solution. Further, the cleaning can be performed by gravity without applying the physical force.

According to some embodiments, after the cleaning is performed, the cleaning liquid containing the impurities may be discharged out of the apparatus for degreasing 100 through the outlet 250. Accordingly, the fat tissue and the fat cells from which at least some of the impurities have been removed may remain in the fat dissolving apparatus 100.

For example, in the process of separating the untreated adipose tissue, adipose tissue, adipose cells, or bulky impurities are caught in the hole of the porous filter membrane 130 to close the hole and reduce the separation efficiency of the device 100 for lipid- .

In some embodiments, when the wash fluid infusion port 290 is in communication with the impurity collection space 170, the wash fluid may first be introduced into the impurity collection space 170 and then into the foliage space 150. The separation efficiency of the fat dissolving apparatus 100 can be recovered by removing the fat tissue, fat cells, or bulky impurities which have been caught in the holes of the porous filter membrane 130 by the cleaning liquid during the movement.

According to exemplary embodiments, the cleaning liquid injection port 290 can be provided in both the foliage space 150 and the impurity collection space 170, and the cleaning liquid can be alternately supplied to the foliage space 150 and the impurity collection space 170 Lt; / RTI > In this case, the fat separation process can be performed while maintaining the separation efficiency of the fat ripening apparatus 100.

The washing solution may include, for example, water, saline solution, Lactated Ringer, Plasmalyte and the like. When the physiological saline solutions are used, they can be washed without damaging adipocytes and adipose tissue. More preferably, saline or lactated ringers can be used.

In some embodiments, the wash liquor may be used in equal or excess amounts relative to the volume of untreated adipose tissue. For example, the washing solution may be used in a volume ratio of 1 to 10 with respect to the untreated fat tissue. It is possible to sufficiently clean the impurities from the untreated fat tissue in the above-mentioned volume ratio range. More preferably, the volume ratio of the washing solution may be 2 to 5 for the untreated fat tissue.

4 is a schematic plan view showing an apparatus for fat ripening according to some embodiments

Referring to FIG. 4, in some embodiments, two or more outlets 250 and 270 may be included in the impurity collection space 170, and a sound pressure pump 310 may be connected to the outlets.

In some embodiments, the outlet 250 may be provided in plurality. Although only two outlets 250 and 270 are shown in FIG. 4, the number of outlets is not limited to two but may be three or more.

For example, when the plurality of outlets 250 and 270 are provided, the impurities can be accelerated to the outside of the fat dissolving apparatus 100 during the fat cell separation process. Accordingly, the rate at which the impurities are passed from the filtering space 150 to the impurity collection space 170 may increase, and thus the rate of the fat cell separation process may increase.

In the exemplary embodiments, the sound pressure pump 310 may be connected to the outlet 250. The sound pressure pump 310 can apply the negative pressure to the fat ripening apparatus 100 to discharge the impurities.

A negative pressure corresponding to a pressure lower than the normal pressure or a vacuum can be applied to the apparatus for fat decoloration 100 through the sound pressure pump 310 and the speed of the fat cell separation process can be accelerated as the stronger sound pressure is applied. In the case of a separation process applying negative pressure, for example, the rate of the fat cell separation process may be increased compared to other separation methods using gravity or the like.

In exemplary embodiments, a negative pressure may be applied to the plurality of outlets 250 and 270 by a sound pressure pump 310, respectively, to perform an adipocyte separation process. In this case, negative pressure is applied to various positions of the impurity collection space 170 according to the positions of the exhaust ports 250 and 270, and negative pressure is applied to the overall area of the porous filter membrane 130, The fat cell separation process can be performed using the area evenly.

For example, when one outlet 250 is used, a portion of the porous membrane 130 near the outlet 250 is blocked by the accumulation of adipocytes during the progress of the fat cell separation process, The above problems can be solved by using the plurality of outlets.

In the exemplary embodiment, when the plurality of outlets 250 and 270 are provided at mutually symmetrical positions with respect to the impurity collection space 170, the entire area of the porous filter membrane 130 can be uniformly utilized, The efficiency of the separation process can be improved.

According to one embodiment, the inlet 210, the withdrawal port 230, and the outlet 250 may each include a lid. The cover can easily seal the injection port 210, the recovery port 230 and the discharge port 250. The structure can be a structure that is locked by rotation in the form of, for example, a bolt or a nut, It may be a structure including a protrusion corresponding to the diameter so that the protrusion closes the hole and is sealed by the pressure.

When the lid is provided, the fat-dispersing device 100 can be sealed and stored without having to transfer the separated fat cells to another container, and the fat cells can be extracted from the fat- .

5 is a schematic side cross-sectional view of an apparatus for fat ripening according to an exemplary embodiment.

5, the porous filter membrane 135 may extend from the one end to the other end across the inner space of the flexible container 110. As shown in FIG.

For example, the porous filter membrane 135 may be provided integrally with the flexible container 110 by being spaced from the upper and lower surfaces of the flexible container 110 and continuously attached to the inner wall surfaces of the four side surfaces.

In one embodiment, the inner space of the flexible container 110 is separated into upper and lower portions by a porous filter membrane 135, the upper portion of which communicates with the injection port 210 to define a filtration space 155, The lower part can communicate with the discharge port 250 to define the impurity collection space 175.

The untreated adipose tissue is injected into the filtering space 155 through the inlet 230 and the impurities are passed to the impurity collection space 175 by the porous filtering membrane 155 and the separated adipose cells are left in the filtering space 155 . The impurities may be discharged to the outside of the flexible container 110 through the discharge port 250 and the separated fat cells may be extracted through the injection port 210 or the recovery port 230.

When the porous membrane 135 extends from the one end to the other end across the inner space of the flexible vessel 110, the internal space of the flexible vessel 110 is utilized as a whole, The fat separation efficiency can be improved by increasing the area.

In addition, when the porous filter membrane 135 is attached to the inner surfaces of the four side surfaces of the flexible container 110, although the outer shape changes repeatedly as the apparatus for degassing 100 is folded and expanded, 135) can maintain the shape without warping, so that a certain fat separation efficiency can be secured.

Embodiments of the present invention provide a method for separating adipocytes using the above-described apparatus 100 for fat ripening.

In some embodiments, the flexible container 110 includes a porous filter membrane 130 disposed within the flexible container 110 to define a filtration space 150, a fountain space 150 from one end of the flexible container 110, And a discharge port 250 disposed at the other end of the flexible container 110 and an injection port 210 communicating with the flexible container 110. [

The untreated adipose tissue may be injected into the filtering space 150 through the injection port 210.

The impurities may be discharged from the untreated fat tissue to the outside of the filtering space 150 and collected into the impurity collection space 170 of the flexible container 110.

The impurities can be removed through the outlet 250.

In one embodiment, the removal of the impurities may be performed by applying a negative pressure to the impurity collection space 170 through the discharge port 250 to discharge the impurities.

With the fat cell separation method described above, fat cells can be extracted or obtained with high purity without physical or mechanical damage.

The fat cells extracted or obtained by the fat splitting device and the fat cell separation method can be used for hip and breast enlargement, wrinkle removal, and scar removal. When the adipocytes are transplanted, the adipocytes may have a reduced probability of side effects such as inflammation reaction at the transplantation site or volume reduction after transplantation.

100: fat splitting device 110: flexible container
130, 135: Porous filter membrane 150, 155: Filtering space
170, 175: impurity collecting space 210: inlet
230: collection port 250: outlet
270: second outlet 290: cleaning liquid inlet
310: Sound pressure pump

Claims (15)

Flexible containers;
A porous filter disposed in the flexible container and defining a filtration space;
An injection port communicating with the filtering space from one end of the flexible container to inject untreated fat tissue; And
And an outlet disposed at the other end of the flexible container for discharging impurities separated from the untreated fat tissue to the outside of the filtration space.
The apparatus according to claim 1, wherein the porous filter membrane is a porous filter bag having a bag shape.
The apparatus of claim 2, wherein the injection port is fused together with the one end of the flexible container and the top of the porous filter bag.
4. The apparatus of claim 3, wherein the porous filter bag has a closed curved shape closed by the fused portion.
5. The apparatus of claim 4, wherein the porous filter bag is disposed to be floating in the space within the flexible container.
The apparatus according to claim 1, wherein the space in the flexible container excluding the filtering space is defined as an impurity collection space.
The fat splitting apparatus according to claim 1, further comprising a cleaning liquid injection port communicated with the foliage space.
7. The apparatus of claim 6, further comprising a cleaning liquid inlet communicated with the impurity collection space.
The fat splitting device according to claim 1, further comprising a recovery port communicating with the filtering space from the one end of the flexible container to collect the adipose tissue or fat cells from which the impurities have been removed from the untreated adipose tissue.
The fat splitting apparatus according to claim 1, wherein the outlet is provided in plurality.
The apparatus of claim 1, further comprising a negative pressure pump coupled to the outlet.
The apparatus of claim 1, wherein the porous filter membrane extends from the one end to the other end across the inner space of the flexible container.
[12] The method of claim 12, wherein the inner space of the flexible container is divided into upper and lower portions by the porous filter membrane,
Wherein the upper portion defines a filtration space communicating with the injection port and the lower portion communicates with the discharge port to define the impurity collection space.
A porous filter disposed in the flexible container and defining a filtration space; an inlet and an outlet communicating with the filtering space from one end of the flexible container; and an outlet disposed at the other end of the flexible container; Preparing an apparatus for fat ripening;
Injecting untreated adipose tissue through the injection port into the foliage space;
Collecting impurities from the untreated fat tissue into the impurity collection space of the flexible container by discharging the impurities out of the filtration space; And
And removing the impurities through the outlet.
15. The method according to claim 14, wherein removing the impurities through the outlet comprises discharging the impurities by applying a negative pressure to the impurity collection space through the outlet.

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