CN110496259B

CN110496259B - Red blood cell collecting and recycling device

Info

Publication number: CN110496259B
Application number: CN201910805713.8A
Authority: CN
Inventors: 夏庆杰; 夏乙清; 徐嘉琴; 张晓�
Original assignee: Chengdu Xingtai Biotechnology Co ltd
Current assignee: Chengdu Xingtai Biotechnology Co ltd
Priority date: 2019-08-29
Filing date: 2019-08-29
Publication date: 2021-09-07
Anticipated expiration: 2039-08-29
Also published as: CN110496259A

Abstract

The invention discloses a red blood cell collecting and recycling device, which comprises a negative pressure device and a processing device, wherein the negative pressure device comprises a first shell, a vacuum pump and a liquid injection pipe, a plurality of hoses are arranged in the first shell, the processing device comprises a second shell and a separating and washing device with a third shell, a first orifice plate and a second orifice plate are arranged in the third shell, the third shell is divided into a washing area and a protective agent storage area by the first orifice plate and the second orifice plate, the third shell is provided with a third orifice plate, the first hose is arranged in the second shell, the second hose is arranged in the washing area, the third hose is arranged in the protective agent storage area, the washing area and the protective agent storage area are respectively provided with a fourth hose and a fifth hose, the fourth hose and the fifth hose are respectively connected with a detergent storage and a protective agent storage, and a Y-shaped pipe is arranged in the second shell. The device can effectively solve the problems that the centrifugal separation time is long, the single separation amount is small and the separated cells can not meet the utilization requirement.

Description

Red blood cell collecting and recycling device

Technical Field

The invention belongs to a red blood cell collecting device, and particularly relates to a red blood cell collecting and recycling device.

Background

With the improvement of medical level and health requirements of people, the requirement of the clinical blood is continuously increased, but at present, on one hand, the problem that the blood source supply is insufficient and the requirement of the clinical blood cannot be met exists; on the other hand, a large amount of bleeding in the operation is not effectively utilized, so that the blood resource is greatly wasted. Although the prior art products can collect and wash the hemorrhage in the art, the prior art basically adopts a step-by-step centrifugation technology for collecting blood and then centrifuging blood cells, which causes the adverse effects of long separation time, damage to the integrity of the cells and the like. Some blood separation pretreatment chips are used for separating blood, but the amount of separated blood is not large, the cost is high, only a small amount of blood can be separated, and the requirement of blood transfusion in the operation cannot be met. The performance of collecting and reusing the intraoperative hemorrhage cannot be achieved in terms of collection and separation of blood alone, so that the technology for collecting, separating and washing the intraoperative hemorrhage is designed and perfected, and the rapid continuous separation of the intraoperative hemorrhage is realized, so that the important significance and value are realized for safe reuse of the intraoperative hemorrhage.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a red blood cell collecting and recycling device which can effectively solve the problems that the existing separation device needs long time for centrifugal separation, has small single separation amount and can not meet the recycling requirement of separated cells.

A red blood cell collecting and recycling device comprises a negative pressure device and a processing device, wherein the negative pressure device comprises a first shell, a vacuum pump and a liquid injection pipe are arranged at the upper part of the first shell, a cover body is arranged on the liquid injection pipe, liquid is contained in an inner shell, a straight pipe, a first hose, a second hose and a third hose are arranged in the first shell, one ends of the straight pipe, the first hose, the second hose and the third hose are all arranged in the liquid, the other ends of the first hose, the second hose and the third hose penetrate through the first shell and are arranged in the processing device, the other end of the straight pipe penetrates through the first shell and is communicated with the atmosphere, and the interior of the first shell is sealed;

the treatment device comprises a second shell and a separation washing device arranged in the middle of the second shell, the separation washing device is connected with the second shell through a support column, the separation washing device comprises a third shell, a first pore plate and a second pore plate are sequentially arranged in the third shell from top to bottom, the third shell is divided into a washing area and a protective agent storage area by the first pore plate and the second pore plate, the protective agent storage area is connected with an external collector through a connecting pipe, a third pore plate is arranged on the side wall of the third shell, and the third pore plate is respectively arranged at the lower parts of the washing area and the protective agent storage area;

the first hose runs through the second casing and sets up inside the second casing, the second hose runs through second casing and third casing and sets up in the washing district, the third hose runs through second casing and third casing and sets up in the district is deposited to the protectant, still be provided with fourth hose and fifth hose in washing district and the district is deposited to the protectant respectively, fourth hose and fifth hose are connected with detergent memory and protectant memory respectively, be provided with the Y venturi tube in the second casing, the Y venturi tube sets up on separation washing unit upper portion, two branches of Y venturi tube are connected with anticoagulant memory and blood suction pipe respectively, the inside sealed that is provided with of second casing.

The beneficial effects produced by adopting the scheme are as follows: vacuum pump and first casing fixed connection are used for carrying out the evacuation in the first casing through the vacuum pump, make and produce the negative pressure in the first casing, and the salt solution of certain concentration and degree of depth is equipped with in the first casing, through the degree of depth of adjustment straight tube, first hose, second hose and third hose in the salt solution, adjusts the negative pressure of its production. The first hose, the second hose, the third hose, the fourth hose, the fifth hose and the Y-shaped pipe are fixedly connected with the second shell.

The third casing, the support column and the second casing are sequentially and fixedly connected, and the working principle of the device is as follows: negative pressure is generated in the first shell under the action of the vacuum pump, and the size of the negative pressure in the first shell is changed by adjusting the depth of one end of the straight pipe in the saline water; a liquid injection pipe with a cover body is arranged, and brine can be supplemented into the first shell through the liquid injection pipe; the first shell and the second shell are communicated through the first hose, so negative pressure is generated in the second shell, under the action of the negative pressure, the anticoagulant in the anticoagulant storage and the blood generated in the operation and adsorbed by the blood sucking tube simultaneously flow into the second shell through the Y-shaped tube, and the anticoagulant is combined with the blood while flowing, so that blood coagulation is avoided; the blood falls on the first pore plate, substances such as red blood cells, blood plasma and the like in the blood flow into the washing area under the action of gravity, blood clots with large volume stay at the upper part of the first pore plate, and negative pressure is generated in the washing area under the action of the second hose as the washing area is communicated with the first shell through the second hose, so that the speed of the blood falling on the first pore plate to be absorbed into the washing area is increased; because the washing area is also communicated with the detergent storage device through the fourth hose, the detergent in the detergent storage device is also absorbed into the washing area under the action of negative pressure, and the detergent can clean blood. A third pore plate is arranged at the lower part of the washing area, most of detergent, plasma, platelets and the like flow into the second shell through the third pore plate, a small amount of plasma, required red blood cells and the like are left in the washing area, and a small amount of red blood cells, plasma, platelets and the like flow into the protective agent storage area through the second pore plate under the action of gravity; because the protective agent storage area is communicated with the first shell through the third hose, negative pressure is generated in the protective agent storage area, the red blood cells in the washing area are accelerated to flow into the protective agent storage area, and meanwhile, because the protective agent storage area is communicated with the protective agent storage device through the fifth hose, under the action of the negative pressure, the cell protective agent also flows into the protection and storage area to be combined with the red blood cells to protect the red blood cells; because the bottom of the protective agent storage area is also provided with the third pore plate, substances such as platelets with small volume further flow away from the protective agent storage area, so that the substances such as platelets in finally collected red blood cells are few, the use requirement can be met, and the substances carrying the red blood cells and the protective agent flow into the collector through the connecting pipe to be collected for subsequent use.

Further, the outer walls of the straight pipe, the first hose, the second hose and the third hose are provided with scales and a plurality of bulges, the scales correspond to the bulges, and the straight pipe, the first hose, the second hose and the third hose are movably connected with the first shell.

The beneficial effects produced by adopting the scheme are as follows: the negative pressure in the first shell can be adjusted by adjusting the depth of the straight pipe in the saline so as to meet the collection requirement; by adjusting the depth of the first hose, the second hose and the third hose in the brine, the negative pressure in the second housing, the washing zone and the protective agent storage zone, respectively, can be adjusted to adjust the speed of adsorption. The top of the first shell is provided with a through hole for inserting the straight pipe, the first hose, the second hose and the third hose, and the diameters of the straight pipe, the first hose, the second hose and the third hose are the same as those of the through hole, so that the first shell is sealed; be provided with the arch, conveniently fix straight tube, first hose, second hose and third hose, improve fixed effect, be provided with the scale, conveniently adjust the degree of depth of straight tube, first hose, second hose and third hose in the salt solution, through the scale alright learn the degree of depth of straight tube, first hose, second hose and third hose in the salt solution.

Furthermore, the upper surface of the first pore plate is inclined, a plurality of filtering pores are arranged on the first pore plate and the second pore plate, and the diameter of each filtering pore is smaller than 15 microns.

The beneficial effects produced by adopting the scheme are as follows: blood flows into washing district through filtering the hole in, sets up the upper surface of first orifice plate into the slope, and when the blood of retrieving in the art fell on first orifice plate, the great clot of volume just slided down along first orifice plate under the action of gravity, realizes carrying out the purpose of separating the clot, avoids the clot to pile up on the surface of first orifice plate, influences the collection of follow-up blood. The diameter of the filter hole is set to be less than 15 mu m, and the diameter of the filter hole is slightly larger than the volume of the red blood cells, so that impurities with the volume larger than that of the red blood cells can be effectively filtered, and the red blood cells can completely flow into the washing area.

Furthermore, a plurality of round holes are arranged on the third orifice plate, and the diameter of each round hole is smaller than 6 mu m.

The beneficial effects produced by adopting the scheme are as follows: the plasma, the small-volume platelets and the like can be discharged through the round hole, and the purity of the collected red blood cells is improved.

Furthermore, a baffle is arranged on the inner wall of the third shell and is respectively arranged at the first hose, the second hose, the fourth hose and the fifth hose.

The beneficial effects produced by adopting the scheme are as follows: the baffle is fixed connection with the third casing, is provided with the baffle, can effectively avoid in liquid stream advances first hose and second hose, improves the health status of first hose, second hose, fourth hose and fifth hose.

Furthermore, the fourth hose and the fifth hose are respectively provided with a switch valve.

The beneficial effects produced by adopting the scheme are as follows: the switch valve is arranged, so that the use convenience is improved, and the discharge of the washing liquid and the cell protective agent can be controlled in time.

Furthermore, the connecting pipe is also provided with a switch valve.

The beneficial effects produced by adopting the scheme are as follows: when more red blood cells are collected in the detergent protection area, the switch valve can be opened to discharge the red blood cells into the collector.

Further, a liquid discharge pipe is arranged at the bottom of the second shell.

The beneficial effects produced by adopting the scheme are as follows: the drain pipe is used for discharging substances such as separated blood clots, detergents, cytoprotective agents, blood plasma and the like into the second housing.

In order to achieve the purpose, the technical scheme adopted by the invention for solving the technical problems is as follows:

the device is provided with a negative pressure device, a straight pipe, a first hose, a second hose and a third hose are arranged in the negative pressure device, the speed of blood adsorption and separation is controlled through the first hose, the second hose and the third hose, a separation and washing device is arranged in the processing device, blood in an operation can be separated and washed simultaneously, the purity of collected blood is improved, and the requirement of recycling is met. Moreover, the device has the advantages of small volume, convenient use and good separation effect, and can realize the purpose of continuously separating and washing the collected blood.

Drawings

FIG. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic view of a portion of the first hose;

reference numerals: 1. a first housing; 2. a vacuum pump; 3. a liquid injection pipe; 4. a liquid; 5. a straight pipe; 6. a first hose; 7. a second hose; 8. a third hose; 9. a second housing; 10. a separation washing device; 11. a support pillar; 12. a third housing; 13. a first orifice plate; 14. a second orifice plate; 15. a washing zone; 16. a protectant storage area; 17. a connecting pipe; 18. a collector; 19. a third orifice plate; 20. a fourth hose; 21. a fifth hose; 22. a detergent reservoir; 23. a protectant reservoir; 24. a Y-shaped tube; 25. calibration; 26. a protrusion; 27. a filtration pore; 28. a circular hole; 29. a baffle plate; 30. an on-off valve; 31. and a liquid discharge pipe.

Detailed Description

The following detailed description of embodiments of the invention refers to the accompanying drawings.

In an embodiment of the present invention, as shown in fig. 1-2, a red blood cell collecting and recycling device is provided, which is characterized by comprising a negative pressure device and a processing device, wherein the negative pressure device comprises a first casing 1, a vacuum pump 2 and a liquid injection tube 3 are arranged on the upper portion of the first casing 1, a cover body is arranged on the liquid injection tube 3, a liquid 4 is contained in the first casing 1, a straight tube 5, a first hose 6, a second hose 7 and a third hose 8 are arranged in the first casing 1, one end of each of the straight tube 5, the first hose 6, the second hose 7 and the third hose 8 is arranged in the liquid 4, the other end of each of the first hose 6, the second hose 7 and the third hose 8 penetrates through the first casing 1 and is arranged in the processing device, the other end of the straight tube 5 penetrates through the first casing 1 and is communicated with the atmosphere, and the inside of the first casing 1 is sealed;

the treatment device comprises a second shell 9 and a separation washing device 10 arranged in the middle of the second shell 9, the separation washing device 10 is connected with the second shell 9 through a support column 11, the separation washing device 10 comprises a third shell 12, a first pore plate 13 and a second pore plate 14 are sequentially arranged in the third shell 12 from top to bottom, the third shell 12 is divided into a washing area 15 and a protective agent storage area 16 through the first pore plate 13 and the second pore plate 14, optimally, the upper surface of the first pore plate 13 is inclined, a plurality of filtering holes 27 are formed in the first pore plate 13 and the second pore plate 14, and the diameters of the filtering holes 27 are smaller than 15 micrometers.

The protective agent storage area 16 is connected with an external collector 18 through a connecting pipe 17, a third pore plate 19 is arranged on the side wall of the third shell 12, and the third pore plate 19 is respectively arranged at the lower parts of the washing area 15 and the protective agent storage area 16; preferably, the third perforated plate 19 is provided with a plurality of circular holes 28, and the diameter of the circular holes 28 is less than 6 μm.

The first hose 6 penetrates through the second shell 9 and is arranged inside the second shell 9, the second hose 7 penetrates through the second shell 9 and the third shell 12 and is arranged in the washing area 15, the third hose 8 penetrates through the second shell 9 and the third shell 12 and is arranged in the protective agent storage area 16, a fourth hose 20 and a fifth hose 21 are further respectively arranged in the washing area 15 and the protective agent storage area 16, the fourth hose 20 and the fifth hose 21 are respectively connected with a detergent storage 22 and a protective agent storage 23, a Y-shaped pipe 24 is arranged inside the second shell 9, the Y-shaped pipe 24 is arranged on the upper portion of the separation washing device 10, two branches of the Y-shaped pipe 24 are respectively connected with an anticoagulant storage and a blood suction pipe, and a seal is arranged inside the second shell 9.

Optimally, the outer walls of the straight pipe 5, the first hose 6, the second hose 7 and the third hose 8 are provided with scales 25 and a plurality of protrusions 26, the scales 25 correspond to the protrusions 26, and the straight pipe 5, the first hose 6, the second hose 7 and the third hose 8 are movably connected with the first shell 1.

Optimally, the inner wall of the third shell 12 is provided with baffles 29, and the baffles 29 are respectively arranged at the first hose 6, the second hose 7, the fourth hose 20 and the fifth hose 21. Preferably, the fourth hose 20 and the fifth hose 21 are respectively provided with an on-off valve 30. Preferably, the connection pipe 17 is also provided with a switching valve 30. Preferably, the bottom of the second housing 9 is provided with a drain 31.

The use process of the device is as follows: starting the vacuum pump 2, generating negative pressure in the first shell 1 under the action of the vacuum pump 2, and changing the size of the negative pressure in the first shell 1 by adjusting the depth of one end of the straight pipe 5 in the saline water; the first casing 1 is communicated with the second casing 9 through the first hose 6, so that negative pressure is generated in the second casing 9, under the action of the negative pressure, the anticoagulant in the anticoagulant storage and the blood generated in the operation adsorbed by the blood sucking tube simultaneously flow into the second casing 9 through the Y-shaped tube 24, and the anticoagulant is combined with the blood to avoid blood coagulation; blood falls on the first orifice plate 13, substances such as red blood cells, blood plasma and the like in the blood flow into the washing area 15 under the action of gravity, blood clots with large volume stay at the upper part of the first orifice plate 13, and negative pressure is generated in the washing area 15 under the action of the second hose 7 as the washing area 15 is communicated with the first shell 1 through the second hose 7, so that the speed of the blood falling on the first orifice plate 13 and being adsorbed into the washing area 15 is accelerated; since the washing area 15 is also communicated with the detergent storage 22 through the fourth hose 20, the detergent in the detergent storage 22 is also absorbed into the washing area 15 under the action of negative pressure, and the detergent can clean the blood. A third pore plate 19 is arranged at the lower part of the washing area 15, most of the detergent, the plasma, the platelets and the like flow into the second shell 9 through the third pore plate 19, a small amount of plasma, required red blood cells and the like remain in the washing area 15, and a small amount of red blood cells, plasma, platelets and the like flow into the protective agent storage area through the second pore plate 14 under the action of gravity; because the protective agent storage area is communicated with the first shell 1 through the third hose 8, negative pressure is also generated in the protective agent storage area, the red blood cells in the washing area 15 are accelerated to flow into the protective agent storage area, and meanwhile, because the protective agent storage area is communicated with the protective agent storage device through the fifth hose 21, under the action of the negative pressure, the cell protective agent also flows into the protection and storage area to be combined with the red blood cells to protect the red blood cells; because the bottom of the protective agent storage area is also provided with the third pore plate 19, substances such as platelets with small volume further flow away from the protective agent storage area, so that few substances such as platelets in finally collected red blood cells can meet the use requirement, and the substances carrying the red blood cells and the protective agent flow into the collector 18 through the connecting pipe 17 to be collected for subsequent use.

Claims

1. The red blood cell collecting and recycling device is characterized by comprising a negative pressure device and a processing device, wherein the negative pressure device comprises a first shell (1), a vacuum pump (2) and a liquid injection pipe (3) are arranged on the upper portion of the first shell (1), a cover body is arranged on the liquid injection pipe (3), liquid (4) is contained in the first shell (1), a straight pipe (5), a first hose (6), a second hose (7) and a third hose (8) are arranged in the first shell (1), one ends of the straight pipe (5), the first hose (6), the second hose (7) and the third hose (8) are arranged in the liquid (4), the other ends of the first hose (6), the second hose (7) and the third hose (8) penetrate through the first shell (1) and are arranged in the processing device, the other end of the straight pipe (5) penetrates through the first shell (1) and is communicated with the atmosphere, the interior of the first shell (1) is sealed; scales (25) and a plurality of bulges (26) are arranged on the outer walls of the straight pipe (5), the first hose (6), the second hose (7) and the third hose (8), the scales (25) are arranged corresponding to the bulges (26), and the straight pipe (5), the first hose (6), the second hose (7) and the third hose (8) are movably connected with the first shell (1);

the treatment device comprises a second shell (9) and a separation washing device (10) arranged in the middle of the second shell (9), the separation washing device (10) is connected with the second shell (9) through a supporting column (11), the separation washing device (10) comprises a third shell (12), a first pore plate (13) and a second pore plate (14) are sequentially arranged in the third shell (12) from top to bottom, the first perforated plate (13) and the second perforated plate (14) divide the third housing (12) into a washing zone (15) and a protective agent storage zone (16), the protective agent storage area (16) is connected with an external collector (18) through a connecting pipe (17), a third orifice plate (19) is arranged on the side wall of the third shell (12), and the third orifice plate (19) is respectively arranged at the lower parts of the washing area (15) and the protective agent storage area (16);

the first hose (6) penetrates through the second shell (9) and is arranged inside the second shell (9), the second hose (7) penetrates through the second shell (9) and the third shell (12) and is arranged inside the washing area (15), the third hose (8) penetrates through the second shell (9) and the third shell (12) and is arranged inside the protective agent storage area (16), a fourth hose (20) and a fifth hose (21) are further arranged inside the washing area (15) and the protective agent storage area (16) respectively, the fourth hose (20) and the fifth hose (21) are respectively connected with a detergent storage (22) and a protective agent storage (23), a Y-shaped pipe (24) is arranged inside the second shell (9), the Y-shaped pipe (24) is arranged on the upper portion of the separation washing device (10), and two branches of the Y-shaped pipe (24) are respectively connected with an anticoagulant storage and a blood sucking pipe, the second shell (9) is internally provided with a seal.

2. The red blood cell collecting and reusing device according to claim 1, wherein the upper surface of the first orifice plate (13) is inclined, a plurality of filtering orifices (27) are provided on each of the first orifice plate (13) and the second orifice plate (14), and the diameter of the filtering orifices (27) is less than 15 μm.

3. The red blood cell collecting and reusing device according to claim 1, wherein the third well plate (19) is provided with a plurality of circular holes (28), and the diameter of the circular holes (28) is less than 6 μm.

4. The red blood cell collecting and reusing device according to claim 1, wherein the inner wall of the third casing (12) is provided with a baffle (29), and the baffles (29) are respectively provided at the first hose (6), the second hose (7), the fourth hose (20) and the fifth hose (21).

5. The red blood cell collecting and reusing device according to claim 1, wherein the fourth tube (20) and the fifth tube (21) are provided with an on-off valve (30), respectively.

6. The red blood cell collecting and reusing device according to claim 5, wherein the on-off valve (30) is also provided on the connection tube (17).

7. The red blood cell collecting and reusing device according to claim 1, wherein a drain tube (31) is provided at the bottom of the second casing (9).