CN116478799A

CN116478799A - Adipose tissue purifying device and purifying method

Info

Publication number: CN116478799A
Application number: CN202310542231.4A
Authority: CN
Inventors: 王伟冲; 杨彦晨; 李春明; 赵鹏; 李晓萌; 隋纹龙; 殷敬华
Original assignee: Shanghai Weigao Medical Technology Development Co ltd
Current assignee: Shanghai Weigao Medical Technology Development Co ltd
Priority date: 2023-05-15
Filing date: 2023-05-15
Publication date: 2023-07-25

Abstract

The invention belongs to the technical field of cell extraction and purification, and discloses a fat tissue purification device and a fat tissue purification method, wherein the device comprises a closed container, a second filtering device and an adsorption device which are arranged in the closed container, the closed container defines a working cavity, the closed container comprises an inlet end and an outlet end, the inlet end is provided with a liquid inlet and a fat inlet which are communicated with the working cavity, the outlet end is provided with a liquid outlet and a fat outlet which are communicated with the working cavity, and the liquid inlet and the liquid outlet are both used for passing through cleaning liquid; the second filtering device is positioned at the outlet end and can cut adipose tissues when extracting fat and control particles of the adipose tissues; the adsorption device is positioned in the working cavity and is used for adsorbing grease in the adipose tissue, and the adipose tissue adsorbed by the adsorption device is discharged through the second filtering device and the fat outlet. The device and the purification method can sufficiently clean and filter the extracted adipose tissues, sufficiently remove grease, improve the purity of the adipose tissues and obtain the adipose particles with the required size.

Description

Adipose tissue purifying device and purifying method

Technical Field

The invention relates to the technical field of cell extraction and purification, in particular to an adipose tissue purification device and a purification method.

Background

Autologous fat has wide availability and histocompatibility, and is considered to be an ideal soft tissue filling material for reducing rejection reactions of the body in filling surgery. The fat at the excessive fat part is transferred to the fat-deficient part, and can be used for facial remodeling, filling up wrinkles and depressions, and the like, without rejection reaction. In addition, osteoarthritis is a chronic disease mainly caused by cartilage degeneration, and damaged cartilage is difficult to repair itself due to the fact that articular cartilage tissue is not supplied with nutrition by blood vessels and the regeneration ability of chondrocytes is poor. Adipose tissue not only produces important proteins associated with cartilage tissue repair and inflammation reduction, but also provides stem cells which can differentiate into chondrocytes under specific induction conditions, and which have excellent self-replication ability and multiple differentiation potential and can promote repair of damaged cartilage. Moreover, adipose tissue tends to remain at the site of injection rather than being immediately resorbed by the body, so that the injected site can be maximally subjected to the benefits of fat injection over a long period of time. In short, the treatment of arthritis and joint injury caused by the abrasion of the joint tissue itself by the patient's own adipose tissue has considerable efficacy.

The essential step in autologous fat transplantation is active purification aspiration of fat particles in order to remove as much as possible blood, anesthetic, interstitial fluid, broken fat and fibrous tissue fragments mixed with intact fat particles. Purification of fat, removal of blood cells and inactive components from aspirate, separation of fat cells from tissue fragments helps to reduce inflammatory reactions in transplantation, thereby increasing adipose tissue survival rate.

Currently, the fat tissue purification methods mainly include a stationary method, a filtration method, a centrifugation method, and the like. The standing method is a layering method by the quality difference of liquid and fat after standing, the standing time is 1-15 minutes, and the method is simple and easy to implement, but has the defects of long material taking time, incomplete impurity precipitation and the like. The filtering method is to wash and filter under aseptic condition with a filter, which can concentrate fat particles and separate them from liquid, grease and fragments, and the subsequent washing procedure can remove residual foreign matters, but still requires long needles to pick out the strip-shaped and fiber connective tissues, which is easy to remove incompletely. Centrifugation is a method of obtaining purified fat by centrifuging a mixture of fat particles by a centrifugal device, and too much centrifugal force may damage the integrity of fat cells, and the centrifugal separation technique is more complicated than the filtration technique and has no advantage in terms of fat viability. In addition, the three methods all enable adipose tissues to be in contact with the outside air for a long time, so that pollution risks exist, and the activity of the adipose cells is influenced.

In addition, in the process of extracting and purifying adipose tissue, a process of removing fat from adipose tissue is generally required, however, the prior art has a defect of incomplete removal when removing fat from a mixture of adipose tissue and fat, or a defect that some adipose tissue is easily removed together when removing fat, resulting in a reduction in the purification extraction rate of adipose tissue.

Accordingly, there is a need for an adipose tissue purification apparatus and purification method to solve the above problems.

Disclosure of Invention

The invention aims to provide a fat tissue purification device and a fat tissue purification method, which can sufficiently clean and filter extracted fat tissues, sufficiently remove grease, improve the purity of the fat tissues and obtain fat particles with required sizes; in addition, in the process of removing the grease, partial adipose tissues can be prevented from being removed together, and the higher fat purification extraction rate is ensured.

To achieve the purpose, the invention adopts the following technical scheme:

in a first aspect, there is provided an adipose tissue purification device comprising:

the closed container is used for defining a working cavity and comprises an inlet end and an outlet end, wherein the inlet end is provided with a liquid inlet and a fat inlet which are communicated with the working cavity, the outlet end is provided with a liquid outlet and a fat outlet which are communicated with the working cavity, and the liquid inlet and the liquid outlet are both used for passing through cleaning liquid;

The second filtering device is arranged in the working cavity and is positioned at the outlet end;

the adsorption device is arranged in the working cavity and is used for adsorbing grease in adipose tissues, and the adipose tissues adsorbed by the adsorption device are discharged through the second filtering device and the fat outlet.

As a preferable scheme of the adipose tissue purifying device provided by the invention, the adsorption device is directly fixed in the working cavity; or alternatively, the process may be performed,

the fat tissue purifying device further comprises a fixing piece, the adsorption device is arranged in the working cavity through the fixing piece, and a fat channel for fat to pass through is arranged on the fixing piece; or alternatively, the process may be performed,

the adsorption device is freely arranged in the working cavity.

As a preferable scheme of the adipose tissue purifying device provided by the invention, the adsorption device is arranged in the working cavity through a fixing piece;

the adsorption device comprises a plurality of adsorption fibers; the fixing piece is provided with a fiber fixing hole, and the adsorption fiber is fixedly arranged in the fiber fixing hole in a penetrating way; and/or the fixing piece comprises an annular frame, one or more fixing rods are arranged in the annular frame, and the adsorption fibers are connected to the fixing rods;

And/or the adsorption device comprises an adsorption column, wherein the adsorption column is of a hollow structure or a solid structure; the fixing piece is provided with an adsorption column mounting cavity, and the adsorption column is inserted into the adsorption column mounting cavity;

and/or the adsorption device comprises an adsorption pipe, wherein the adsorption pipe comprises two arc-shaped sheets which are arranged at intervals and opposite to each other; the fixing piece is provided with an arc piece installation cavity, and the arc piece is inserted into the arc piece installation cavity;

and/or the adsorption device comprises an adsorption sheet, wherein one adsorption sheet or a plurality of adsorption sheets are circumferentially distributed by taking the axis of the working cavity as the center; the fixing piece is provided with one or more adsorption piece fixing cavities, and the adsorption piece is inserted into the adsorption piece fixing cavities.

As the preferable scheme of the adipose tissue purifying device provided by the invention, the fixing piece is provided with the first positioning structure, the inner wall of the working cavity is provided with the second positioning structure, and the first positioning structure is matched with the second positioning structure so that the fixing pieces at two ends of the adsorption device are completely opposite.

As the preferable scheme of the fat tissue purifying device provided by the invention, the adsorption device comprises an adsorption ball which is fixedly arranged in the working cavity or freely arranged in the working cavity.

The fat tissue purifying device provided by the invention further comprises a third filtering device, wherein the third filtering device is arranged in the working cavity and positioned at the outlet end, the outlet end is further provided with a fat outlet, the third filtering device is used for allowing fat to pass through and blocking fat tissue from passing through, and the fat outlet is used for discharging the fat filtered by the third filtering device.

As a preferable scheme of the fat tissue purifying device provided by the invention, the third filtering device is positioned between the second filtering device and the fat outlet, a first through hole and a second through hole are arranged on the third filtering device, the liquid outlet is communicated with the working cavity through the first through hole, and the fat outlet is communicated with the working cavity through the second through hole;

or the second filtering device and the third filtering device are of an integrated structure and are positioned on the same plane, the second filtering device and the third filtering device are separated by a separation plate, the separation plate is in sealing contact with the inner wall of the closed container, the separation plate can allow grease to pass through and block fat tissues from passing through, the liquid outlet and the fat outlet are opposite to the second filtering device, and the grease outlet is opposite to the third filtering device.

The fat tissue purifying device provided by the invention further comprises a third filtering device and a fat outlet, wherein the third filtering device is arranged on the fat outlet and detachably connected with the fat outlet, the third filtering device is used for allowing fat to pass through and blocking fat tissue from passing through, and the fat outlet is used for discharging the fat filtered by the third filtering device.

As the preferable scheme of the adipose tissue purifying device provided by the invention, the inlet end is provided with a first tee joint structure, the liquid inlet is a first inlet of the first tee joint structure, the adipose inlet is a second inlet of the first tee joint structure, and the outlet of the first tee joint structure is communicated with the working cavity;

and/or the outlet end is provided with a second tee structure, an inlet of the second tee structure is communicated with the working cavity, the liquid outlet is a first outlet of the second tee structure, and the fat outlet is a second outlet of the second tee structure.

As the preferable scheme of the adipose tissue purification device provided by the invention, the liquid inlet and the liquid outlet are provided with the adjusting device for adjusting the flow;

And/or check valves are arranged at the fat inlet and the fat outlet;

and/or, the fat inlet and the fat outlet are provided with luer locks.

As a preferable scheme of the adipose tissue purifying device provided by the invention, a stirring device is arranged in the working cavity.

As a preferable scheme of the adipose tissue purification device provided by the invention, the adsorption device is of a porous structure; and/or the adsorption device is made of oleophilic and hydrophobic materials.

As a preferable scheme of the adipose tissue purifying device provided by the invention, the adipose tissue purifying device further comprises a first filtering device, wherein the first filtering device is arranged in the working cavity and positioned at the inlet end, the filtering hole of the second filtering device is smaller than the filtering hole of the first filtering device, and the adsorption device is positioned between the first filtering device and the second filtering device.

In a second aspect, there is provided an adipose tissue purification method applied to the adipose tissue purification device as described above, the adipose tissue purification method comprising the steps of:

s1, liquid injection and exhaust: filling the working cavity of the closed container with clean cleaning liquid through the liquid inlet to discharge air in the working cavity;

S2, injecting unrefined adipose tissue: injecting unrefined adipose tissue into the working chamber through the fat inlet;

s3, sucking out grease: the closed container is shaken to mix the adipose tissues and the cleaning solution, and the adsorption device adsorbs grease during the process;

s4, washing fat: standing to delaminate the adipose tissues and the impurity-containing cleaning solution, discharging the impurity-containing cleaning solution through a liquid outlet, and injecting a clean cleaning solution through a liquid inlet;

s5, repeating the steps S3 and S4 until the discharged cleaning liquid becomes clear;

s6, collecting fat: purified adipose tissue was collected through the adipose outlet.

As a preferred embodiment of the method for purifying adipose tissue provided by the present invention, after step S5, step S60 is further included before step S6: residual grease is discharged through the grease outlet.

In the step S1, the liquid inlet is positioned at the bottom end and the liquid outlet is positioned at the top end when the liquid is injected and exhausted;

and/or, in step S2, inverting the adipose tissue purification device so that the adipose inlet is at the top while injecting the unrefined adipose tissue;

and/or, in step S60 and step S6, the fat outlet and the fat outlet are at the top end;

And/or, in step S60 and step S6, injecting liquid into the working cavity through the fat inlet and/or the liquid inlet for pressurizing so as to discharge fat tissues and grease.

As a preferable scheme of the fat tissue purification method provided by the invention, the cleaning liquid is physiological saline.

In a third aspect, there is provided an adipose tissue purification apparatus comprising:

the closed container is used for defining a working cavity and comprises an inlet end and an outlet end, wherein the inlet end is provided with a liquid inlet and a fat inlet which are communicated with the working cavity, the outlet end is provided with a liquid outlet, a fat outlet and a fat outlet which are communicated with the working cavity, and the liquid inlet and the liquid outlet are both used for passing through cleaning liquid;

the second filtering device is arranged in the working cavity and positioned at the outlet end, and adipose tissues can be discharged through the second filtering device and the adipose outlet;

the third filtering device is arranged in the working cavity or outside the working cavity, the third filtering device is used for allowing grease to pass through and blocking fat tissues from passing through, and the grease outlet is used for discharging the grease filtered by the third filtering device.

As a preferable mode of the adipose tissue purifying device provided by the invention, the adipose tissue purifying device further comprises an adsorption device, wherein the adsorption device is arranged in the working cavity and is used for adsorbing grease in adipose tissue, and the adipose tissue adsorbed by the adsorption device is discharged through the second filtering device and the adipose outlet.

In a fourth aspect, there is provided an adipose tissue purification method applied to the adipose tissue purification device as described above, the adipose tissue purification method comprising the steps of:

w1, liquid injection and exhaust: filling the working cavity of the closed container with clean cleaning liquid through a liquid inlet so as to discharge air in the working cavity;

w2, injection into unrefined adipose tissue: injecting unrefined adipose tissue into the working chamber through a fat inlet;

w3, rinse fat: standing after shaking to layer fat tissues and cleaning liquid containing impurities, discharging the cleaning liquid containing impurities through a liquid outlet, and injecting clean cleaning liquid through the liquid inlet;

w4, repeating the step W3 until the discharged cleaning liquid becomes clear;

w5, discharging grease: discharging the grease filtered by the third filtering device through the grease outlet;

W6, collecting fat: purified adipose tissue was collected through the adipose outlet.

The invention has the beneficial effects that:

the invention provides a fat tissue purifying device and a fat tissue purifying method, which can clean, filter and adsorb fat tissues, the whole fat purifying process is carried out in a closed container, the fat tissues are prevented from being contacted with external air during purification, and compared with the traditional standing, filtering and centrifuging methods, the fat tissue purifying device and the fat tissue purifying method eliminate the probability of air infection. The fat tissue enters the closed container through the fat inlet, the cleaning liquid is injected into the working cavity through the liquid inlet, blood, anesthetic liquid, interstitial fluid and the like mixed with complete fat particles can be removed after the cleaning liquid is fully mixed with the fat tissue, and simultaneously, the adsorption device can adsorb grease in the fat tissue mixed liquid when the cleaning liquid is fully mixed with the fat tissue, so that the aim of removing the grease is fulfilled. Compared with the prior art, the fat tissue purification device can absorb the fat in the fat extraction mixture to the greatest extent, and avoid the phenomenon that the subsequent fat implantation part generates inflammatory reaction due to the residual fat. The fat tissue adsorbed via the adsorption device is discharged through the second filtering device and the fat outlet, and the second filtering device is capable of cutting the fat tissue to control particles of the fat tissue when extracting fat. The fat tissue purifying device and the purifying method can sufficiently clean and filter the extracted fat mixture, sufficiently remove grease, improve the purity of fat tissue and obtain fat particles with required size.

Drawings

Fig. 1 is an overall view of an adipose tissue purifying apparatus provided in accordance with an embodiment of the present invention;

FIG. 2 is a schematic view of an adipose tissue purifying device according to an embodiment of the present invention after hiding a cylinder;

fig. 3 is a cross-sectional view of an adipose tissue purifying apparatus according to an embodiment of the present invention;

FIG. 4 is a schematic view of a first filtering device according to an embodiment of the present invention;

FIG. 5 is a schematic view of a second filter device according to an embodiment of the present invention;

FIG. 6 is a schematic structural view of a fixing member according to a first embodiment of the present invention;

fig. 7 is an overall view of an adipose tissue purifying apparatus provided in the second embodiment of the present invention;

fig. 8 is a cross-sectional view of an adipose tissue purifying apparatus according to a second embodiment of the present invention;

fig. 9 is a schematic structural diagram of a third filtering device according to a second embodiment of the present invention;

fig. 10 is a sectional view of an adipose tissue purifying apparatus according to a third embodiment of the present invention;

FIG. 11 is a schematic structural view of a fixing member according to a third embodiment of the present invention;

fig. 12 is a schematic view of a fat tissue purifying device according to a fourth embodiment of the present invention after hiding a cylinder;

fig. 13 is a sectional view of an adipose tissue purifying apparatus according to a fourth embodiment of the present invention;

Fig. 14 is a schematic structural view of a fixing member according to a fourth embodiment of the present invention;

fig. 15 is a schematic view of a fat tissue purifying device according to a fifth embodiment of the present invention after hiding a cylinder;

FIG. 16 is a sectional view of an adipose tissue purifying apparatus according to a fifth embodiment of the present invention;

fig. 17 is a schematic structural view of a fixing member according to a fifth embodiment of the present invention;

fig. 18 is a sectional view of an adipose tissue purifying apparatus according to an eighth embodiment of the present invention;

FIG. 19 is a schematic view showing a structure in which a second filter device and a third filter device are integrated together according to an eighth embodiment of the present invention;

fig. 20 is an overall view of an adipose tissue purifying apparatus provided in a ninth embodiment of the present invention;

fig. 21 is a sectional view of an adipose tissue purifying apparatus provided in a ninth embodiment of the present invention;

fig. 22 is an overall view of an adipose tissue purifying apparatus provided in the tenth embodiment of the present invention;

fig. 23 is a cross-sectional view of an adipose tissue purifying apparatus according to a tenth embodiment of the present invention;

FIG. 24 is an enlarged view of a portion of FIG. 23 at A;

FIG. 25 is an isometric view of an oil extraction structure comprising a third filter arrangement and a grease outlet according to a tenth embodiment of the present invention;

FIG. 26 is a cross-sectional view of an oil delivery structure provided in accordance with a tenth embodiment of the present invention;

FIG. 27 is a schematic view of a filter element of a third filter arrangement provided in accordance with a tenth embodiment of the invention;

FIG. 28 is an internal view of a first adipose tissue purifying apparatus provided in accordance with an eleventh embodiment of the present invention;

FIG. 29 is a cross-sectional view of a first adipose tissue purifying apparatus provided in accordance with an eleventh embodiment of the present invention;

FIG. 30 is an internal view of a second adipose tissue purifying apparatus according to an eleventh embodiment of the present invention;

FIG. 31 is a sectional view of a second adipose tissue purifying apparatus according to an eleventh embodiment of the present invention;

FIG. 32 is an internal view of a third adipose tissue purifying apparatus provided in accordance with an eleventh embodiment of the present invention;

FIG. 33 is a cross-sectional view of a third adipose tissue purifying apparatus provided in accordance with an eleventh embodiment of the present invention;

FIG. 34 is a flowchart of a method for purifying adipose tissue according to a twelfth embodiment of the present invention;

FIG. 35 is a schematic view of the process of liquid injection and gas discharge in the adipose tissue purification method;

FIG. 36 is a schematic view showing a process of injecting unrefined adipose tissue in the adipose tissue purification method;

FIG. 37 is a schematic view showing a process of shaking a closed vessel to sufficiently adsorb fat in the method of purifying adipose tissue;

FIG. 38 is a schematic view showing a process of washing fat in the fat tissue purification method;

FIG. 39 is a schematic view showing a process of discharging residual fat in the fat tissue purifying method;

FIG. 40 is a schematic view showing a process of collecting purified adipose tissue in the adipose tissue-purifying method;

FIG. 41 is a cross-sectional view of a first adipose tissue purifying apparatus according to a thirteenth embodiment of the present invention;

FIG. 42 is a cross-sectional view of a second adipose tissue purifying apparatus according to a thirteenth embodiment of the present invention;

FIG. 43 is a cross-sectional view of a third adipose tissue purifying apparatus according to a thirteenth embodiment of the present invention;

fig. 44 is a cross-sectional view of a fourth adipose tissue purifying apparatus according to a thirteenth embodiment of the present invention.

In the figure:

1. a closed container; 2. a first filtering device; 3. a second filtering device; 5. a fixing member; 6. a third filtering device; 7. an adjusting device; 8. a check valve;

11. a working chamber; 12. a cylinder; 13. an inlet end cap; 14. an outlet end cap;

131. a liquid inlet; 132. a fat inlet; 141. a liquid outlet; 142. a fat outlet; 143. a grease outlet;

21. a first filter aperture; 31. a second filter aperture;

41. adsorbing fibers; 42. an adsorption column; 43. an adsorption tube; 44. an adsorption sheet;

431. an arc-shaped sheet;

51. a fat channel; 52. a fiber fixing hole; 53. an annular frame; 54. a fixed rod; 55. an adsorption column mounting cavity; 56. an arc piece installation cavity; 57. an adsorption sheet fixing cavity; 58. a first positioning structure;

61. A third filter aperture; 62. a first via; 63. a second via; 64. a partition plate; 65. a filter element.

Detailed Description

The invention is described in further detail below with reference to the drawings and examples. It is to be understood that the specific embodiments described herein are merely illustrative of the invention and are not limiting thereof. It should be further noted that, for convenience of description, only some, but not all of the structures related to the present invention are shown in the drawings.

In the description of the present invention, unless explicitly stated and limited otherwise, the terms "connected," "connected," and "fixed" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In the present invention, unless expressly stated or limited otherwise, a first feature "above" or "below" a second feature may include both the first and second features being in direct contact, as well as the first and second features not being in direct contact but being in contact with each other through additional features therebetween. Moreover, a first feature being "above," "over" and "on" a second feature includes the first feature being directly above and obliquely above the second feature, or simply indicating that the first feature is higher in level than the second feature. The first feature being "under", "below" and "beneath" the second feature includes the first feature being directly under and obliquely below the second feature, or simply means that the first feature is less level than the second feature.

In the description of the present invention, the terms "upper," "lower," "left," "right," and the like are used for convenience of description and simplicity of operation based on the orientation or positional relationship shown in the drawings, and do not denote or imply that the apparatus or element in question must have a particular orientation, be constructed and operated in a particular orientation, and thus should not be construed as limiting the invention. Furthermore, the terms "first," "second," and the like, are used merely for distinguishing between descriptions and not for distinguishing between them.

In the prior art, the standing method and the filtering method for purifying the adipose tissues have the defect of incomplete impurity removal, the centrifugation method and the like have the defect of destroying the integrity of the adipose cells, and the standing method, the filtering method and the centrifugation method all enable the adipose tissues to contact with the outside air for a long time, so that pollution risks exist and the activity of the adipose cells is influenced. In addition, there is also a technical scheme of stirring emulsified oil by using special alloy balls as stirring elements to realize removal of oil, but the stable emulsification effect is difficult to obtain only by stirring without adding components such as an emulsifier and the like only by means of normal saline, and after standing separation, oil and water quickly delaminate, so that the oil is difficult to thoroughly remove, and the defects of insufficient emulsification and insufficient free oil removal exist.

Based on the technical problems, the invention provides the adipose tissue purification device which can sufficiently clean and filter the extracted adipose tissues, sufficiently remove the grease, improve the purity of the adipose tissues and obtain the adipose particles with the required size.

The adipose tissue purifying apparatus according to the present invention will be described in detail with reference to several specific embodiments, and the differences between the embodiments will be emphasized, and the same similar parts between the embodiments can be seen from the following description of the embodiments of the present invention, which is not to be construed as limiting the scope of the present invention, but any changes and modifications based on the disclosure of the present invention are within the scope of the present invention.

Example 1

Fig. 1 to 6 are schematic views of an adipose tissue purifying apparatus provided in the present embodiment. As shown in fig. 1, 2 and 3, the adipose tissue purifying apparatus includes a closed vessel 1, a second filtering device 3 and an adsorption device.

The closed vessel 1 defines a working chamber 11, the closed vessel 1 comprising an inlet end and an outlet end, see fig. 3, the lower end of the closed vessel 1 being the inlet end and the upper end being the outlet end. The inlet end is provided with a liquid inlet 131 and a fat inlet 132 which are communicated with the working chamber 11, the outlet end is provided with a liquid outlet 141 and a fat outlet 142 which are communicated with the working chamber 11, the liquid inlet 131 and the liquid outlet 141 are both used for passing through cleaning liquid, and the fat inlet 132 and the fat outlet 142 are both used for passing through fat. The second filter device 3 is arranged in the working chamber 11 and at the outlet end. In collecting the final purified fat, the fat tissue is discharged from the fat outlet 142 through the second filtering device 3, and the second filtering device 3 serves to reduce the size of the fat tissue and to limit the collection of the fat tissue of a certain size. The adsorption device is disposed in the working chamber 11, and is used for adsorbing the grease in the adipose tissue, and the adipose tissue adsorbed by the adsorption device is discharged through the second filtering device 3 and the fat outlet 142.

From the above, the fat tissue purifying device can clean, filter and adsorb fat tissue, and the whole fat purifying process is carried out in the closed container 1, so that the fat tissue is prevented from contacting with external air during purification, and compared with the traditional standing, filtering and centrifuging methods, the fat tissue purifying device eliminates the probability of air infection. Fat tissue enters the closed container 1 through the fat inlet 132, cleaning liquid is injected into the working cavity 11 through the liquid inlet 131, blood, anesthetic liquid, interstitial fluid and the like mixed with complete fat particles can be removed after the cleaning liquid is fully mixed with the fat tissue, and simultaneously, when the cleaning liquid is fully mixed with the fat tissue, the adsorption device can adsorb grease in the fat tissue mixed liquid, so that the aim of grease removal is fulfilled.

Compared with the technology that the emulsification is needed to discharge excessive grease and other impurities, the grease in the fat extraction mixture can be sucked and removed to the greatest extent, and the phenomenon that the grease residue is caused by insufficient emulsification and then the follow-up fat implantation part generates inflammatory reaction is avoided. The fat tissue adsorbed via the adsorption device is discharged through the second filtering device 3 and the fat outlet 142, and the second filtering device 3 can further cut the fat tissue to control particles of the fat tissue when extracting fat. The fat tissue purifying device and the purifying method can sufficiently clean and filter the extracted fat mixture, sufficiently remove grease, improve the purity of fat tissue and obtain fat particles with required size.

Preferably, as shown in fig. 3, the adipose tissue purifying device further comprises a first filtering device 2, the first filtering device 2 is disposed in the working chamber 11 and located at the inlet end, the filtering hole of the second filtering device 3 is smaller than the filtering hole of the first filtering device 2, and the adsorbing device is located between the first filtering device 2 and the second filtering device 3.

The space between the first filter device 2 and the second filter device 3 is defined as a refining chamber, the freshly extracted adipose tissue must pass through the first filter device 2 to enter the refining chamber, the first filter device 2 is used for preventing oversized fibrous tissue from entering the refining chamber, and the space serves to chop the adipose tissue and make the adipose tissue particles smaller. That is, the fat introduced through the fat inlet is primarily filtered through the first filter 2 to chop the fat while intercepting a portion of the fibrous tissue, and the primarily filtered fat is introduced between the first filter 2 and the second filter 3, and then the fat in the adipose tissue is adsorbed by the adsorption device. The fat tissue from which the fat has been sucked is further minced by means of the second filter device 3 to obtain fat particles of the desired size.

In an alternative embodiment, the adsorption means may be fixed directly in the working chamber 11, for example to the chamber wall of the working chamber 11, or to the first filter means 2 and the second filter means 3, as long as the normal filtering function of the first filter means 2 and the second filter means 3 is not affected.

In a new embodiment, the adsorption means may be mounted in the working chamber 11 by means of a fixture 5, the fixture 5 being provided with a fat channel 51 for the passage of fat. Adipose tissue enters the purification chamber via the first filtering means 2 and the fat channel 51 on the fixing element 5

In addition, there is also an embodiment in which the adsorption means is not fixed in the working chamber 11, and is freely movable as long as the purpose of adsorbing the grease in the adipose tissue-mixed solution can be achieved.

Wherein the adsorption device is arranged in the working chamber 11 through the fixing piece 5. Referring to fig. 2 and 3, specifically, the adsorption device includes a plurality of adsorption fibers 41, the extension direction of the adsorption fibers 41 is consistent with the axial direction of the closed casing 1, and both ends of the adsorption fibers 41 are fixed in the working chamber 11 by the fixing members 5.

In an alternative embodiment, referring to fig. 6 (c 1), the fixing member 5 is formed in a plate shape as a whole, and the fiber fixing holes 52 are provided thereon, and the adsorption fibers 41 are inserted and fixed in the fiber fixing holes 52. That is, the upper end of the adsorption fiber 41 is inserted into the fiber fixing hole 52 of the upper fixture 5, and the lower end is inserted into the fiber fixing hole 52 of the lower fixture 5, thereby fixing the adsorption fiber 41 in the working chamber 11. The fixing piece 5 is provided with a plurality of fat channels 51, so that fat can pass through conveniently, and the fat channels 51 can be uniformly distributed circumferentially.

In another alternative embodiment, referring to fig. 6 (c 2), the fixing member 5 includes a ring frame 53, and a plurality of fixing rods 54 are disposed in the ring frame 53, and the adsorption fibers 41 are connected to the fixing rods 54 (e.g., bound). Illustratively, a plurality of fixing rods 54 are uniformly distributed circumferentially around the center of the annular frame 53, and a plurality of adsorption fibers 41 may be fixed to each fixing rod 54. Between two adjacent fixing bars 54 are fat channels 51.

Alternatively, the fixing member 5 may further include an annular frame 53 and a fixing plate disposed in the annular frame 53, but the fixing plate does not fill the entire inner space of the annular frame 53, a plurality of fiber fixing holes 52 are formed in the fixing plate, and one or more fixing rods 54 are disposed in a region where the fixing plate is not disposed, that is, the fixing member 5 is provided with both the fiber fixing holes 52 and the fixing rods 54, and fixing of the adsorption fiber 41 may also be achieved.

Preferably, referring to fig. 6, the fixing member 5 is provided with a first positioning structure 58, the inner wall of the working cavity 11 is provided with a second positioning structure, and the first positioning structure 58 and the second positioning structure cooperate to make the fixing members 5 at the upper end and the lower end of the adsorption device completely face each other, so that the adsorption fiber 41 is convenient to install and fix. Illustratively, the first positioning structure 58 is a protrusion protruding from a side portion of the fixing element 5, and the second positioning structure is a groove disposed on an inner wall of the working cavity 11, and the protrusion is clamped into the groove to position the fixing element 5. Alternatively, the first positioning structure 58 is a groove, and the second positioning structure is a protrusion, so that the positioning of the fixing member 5 can be achieved.

Preferably, the adsorption fiber 41 is a material filled with voids, and further, may be a hollow fiber or a porous structure. More preferably, the absorbent fiber 41 is an oleophilic and hydrophobic material, and the absorbent fiber 41 is exemplified by one of polypropylene, polyurethane, polyethylene, polytetrafluoroethylene, polyester and other polymer materials.

Alternatively, the adsorption fiber 41 may be a monofilament, a multifilament, a monofilament of different materials, or an adsorption device formed by different fibers and different materials.

In this embodiment, the diameter of the adsorbent fibers 41 is preferably between 0.5mm and 2 mm.

Alternatively, the inner diameter of the closed vessel 1 (i.e. the diameter of the working chamber 11) may be 20mm-80mm, further preferably between 40mm-60 mm.

Alternatively, as shown in fig. 4 (a 1), the first filter hole 21 may be a circular hole; as shown in fig. 4 (a 2), the first filter holes 21 may be hexagonal holes; as shown in fig. 4 (a 3), the first filter holes 21 may be square holes.

Alternatively, as shown in fig. 5 (b 1), the second filter hole 31 may be a circular hole; as shown in fig. 5 (b 2), the second filtering holes 31 may be square holes; as shown in fig. 5 (b 3), the second filter holes 31 may be hexagonal holes.

In this embodiment, the size of the first filter holes 21 on the first filter device 2 is larger than the size of the second filter holes 31 on the second filter device 3. Preferably, the first filter holes 21 have a size of between 3mm and 5mm and the second filter holes 31 have a size of between 0.3mm and 3 mm. More preferably, the second filter holes 31 have a size between 0.5mm and 2.4 mm. If the filter holes are round holes, the dimensions refer to the diameters thereof, and if the filter holes are square holes or hexagonal holes, the dimensions refer to the diameters of the outer circles thereof.

Preferably, the first filter device 2 and/or the second filter device 3 are of a hard material, for example a metal or a polymeric material. The first filter device 2 and/or the second filter device 3 are illustratively stainless steel or an aluminum alloy or ABS hard material or the like.

Optionally, referring to fig. 3, an adjusting device 7 for adjusting the flow rate is provided at each of the liquid inlet 131 and the liquid outlet 141. The adjusting means 7 may be an adjusting valve, such as a ball valve, a plug valve, etc.; alternatively, the adjusting device 7 may be a clip, by which the flow adjustment at the liquid inlet 131 and the liquid outlet 141 may also be achieved.

Optionally, check valves 8 are provided at both the fat inlet 132 and the fat outlet 142, the check valves 8 preferably being silicone valves. By providing the check valve 8, fat backflow can be prevented, and adverse effects on the fat purification process can be avoided.

Optionally, luer locks are provided on both the fat inlet 132 and the fat outlet 142. Specifically, when the fat just extracted is injected, the fat injection device is connected with the fat inlet 132 through a luer lock, so that the fat inlet 132 is prevented from being broken under pressure; when the purified fat is collected, the fat collecting device is connected with the fat outlet 142 through a luer lock, so that the fat outlet 142 is prevented from being broken under pressure.

Referring to fig. 1, 2 and 3, the closed container 1 includes a cylinder 12 with two open ends, a working chamber 11 is disposed inside the cylinder 12, two ends of the cylinder 12 are respectively provided with an inlet end cover 13 and an outlet end cover 14, a liquid inlet 131 and a fat inlet 132 are both disposed on the inlet end cover 13, and a liquid outlet 141 and a fat outlet 142 are both disposed on the outlet end cover 14. Preferably, both the inlet end cap 13 and the outlet end cap 14 are detachably connected (e.g. screwed) to the end of the cartridge 12, facilitating the installation and replacement of the first filter device 2, the second filter device 3 and the adsorption device inside.

Further, the closed vessel 1 is preferably made of a transparent material, so that the internal fat state can be easily observed.

Example two

As shown in fig. 7, 8 and 9, the present embodiment provides an adipose tissue purifying apparatus which is further improved on the basis of the first embodiment.

In this embodiment, referring to fig. 8, the adipose tissue purifying device further comprises a third filtering device 6, and the third filtering device 6 is disposed in the working chamber 11 and located at the outlet end. The outlet end is also provided with a grease outlet 143. The third filtering means 6 is for allowing the grease to pass therethrough while blocking the passage of fat tissue, and the grease outlet 143 is for discharging the grease filtered by the third filtering means 6, which is the grease not adsorbed by the adsorbing means, i.e., the residual grease. Since the third filtering means 6 allows only the grease to pass therethrough while blocking the passage of fat tissue, the residual grease can pass through the third filtering means 6 and be discharged through the grease outlet 143 before collecting the fat, further improving the purity of the fat.

Referring to fig. 9, the third filtering means 6 is provided with a third filtering hole 61, and the third filtering hole 61 has a smaller size than the second filtering hole 31, which allows only grease to pass therethrough and does not allow fat to pass therethrough. The third filter hole 61 should have a size smaller than that of fat cells, thereby blocking the passage of fat. Preferably, the size of the third filter holes 61 is not more than 10 μm, more preferably, the size of the third filter holes 61 is not more than 5 μm.

Referring to fig. 8 and 9, in the present embodiment, the third filtering device 6 is located between the second filtering device 3 and the grease outlet 143, the third filtering device 6 is provided with a first through hole 62 and a second through hole 63, the liquid outlet 141 is communicated with the working chamber 11 through the first through hole 62, and the grease outlet 142 is communicated with the working chamber 11 through the second through hole 63. The cleaning liquid enters the liquid outlet 141 through the first through hole 62 so as to be discharged through the liquid outlet 141; adipose tissue enters the fat outlet 142 through the second via hole 63 so as to be discharged through the fat outlet 142.

Preferably, the third filter means 6 is a hard material, for example a metal or a polymeric material. The third filter means 6 is illustratively stainless steel or an aluminium alloy or ABS hard material or the like.

Optionally, referring to fig. 8, a check valve 8 is provided at the grease outlet 143 to prevent the grease from flowing backward.

Optionally, a luer lock is provided at the grease outlet 143 to prevent the grease outlet 143 from collapsing under pressure when grease is discharged.

Example III

As shown in fig. 10 and 11, the present embodiment provides an adipose tissue purifying apparatus, which is different from the first and second embodiments in that: the adsorption device comprises an adsorption column 42, wherein the adsorption column 42 is of a hollow structure or a solid structure and is used for adsorbing grease in adipose tissues. The diameter of the adsorption column 42 is far larger than that of the adsorption fiber 41, so that the strength is higher and the adsorption column is less susceptible to damage.

Both ends of the adsorption column 42 are mounted in the working chamber 11 through the fixing members 5. Referring specifically to fig. 10 and 11, the fixing member 5 is provided with an adsorption column mounting cavity 55, and the adsorption column 42 is inserted into the adsorption column mounting cavity 55 to fix the adsorption column 42 in the working cavity 11. Further, a plurality of fat passages 51 are provided on the outer periphery of the adsorption column mounting chamber 55 on the fixing member 5, and a convex first positioning structure 58 is also provided on the outer side of the fixing member 5.

The diameter of the adsorption column 42 may be between 5mm and 40mm, preferably between 10mm and 30 mm.

Preferably, the adsorption column 42 is a void-filling material. Further, the adsorption column 42 may have a hollow structure or a porous structure, and more preferably, the adsorption column 42 is made of an oleophilic and hydrophobic material. Illustratively, the material of the adsorption column 42 is one of polypropylene, polyurethane, polyethylene, polytetrafluoroethylene, polyester, and other polymer materials.

Example IV

As shown in fig. 12, 13 and 14, the present embodiment provides an adipose tissue purifying apparatus, which is different from the first, second and third embodiments in that: the adsorption device comprises an adsorption tube 43, the adsorption tube 43 comprises two arc-shaped sheets 431 which are arranged at intervals and opposite to each other, and the arc-shaped sheets 431 are used for adsorbing grease in adipose tissues. The contact area of the adsorption sheet 44 and the adipose tissue is larger than that of the adsorption column 42 and the adipose tissue, and the adsorption of the grease is facilitated. Furthermore, a gap is left between the two arcuate tabs 431, which facilitates the operator's observation of the fat state within the working chamber 11.

Both ends of the arc-shaped piece 431 are installed in the working cavity 11 through the fixing piece 5. Referring to fig. 14, the fixing member 5 is provided with an arc piece mounting cavity 56, and the arc pieces 431 are inserted into the arc piece mounting cavity 56, so as to fix the two arc pieces 431 in the working cavity 11. The two arc piece mounting cavities 56 on the fixing piece 5 are close to the outer side edge of the fixing piece 5, so that the two arc pieces 431 are attached to the inner wall of the closed container 1 as much as possible, and fat is sucked between the two arc pieces 431. Preferably, the outer diameter of the adsorption tube 43 is identical to the inner diameter of the cylinder 12 of the closed vessel 1, and the wall thickness of the adsorption tube 43 is 1mm to 5mm, preferably 2mm to 4mm, so that the adsorption tube has not only sufficient strength but also a large adsorption area.

A plurality of fat passages 51 are provided in the fixing member 5 between the two arc piece installation cavities 56, and a convex first positioning structure 58 is also provided on the outer side of the fixing member 5.

Preferably, the arcuate tabs 431 of the sorbent tube 43 are void-filled materials, and further, may be hollow or porous. More preferably, the arc-shaped piece 431 is made of a lipophilic and hydrophobic material, and the arc-shaped piece 431 is made of one of polypropylene, polyurethane, polyethylene, polytetrafluoroethylene, polyester and other polymer materials.

Example five

As shown in fig. 15, 16 and 17, the present embodiment provides an adipose tissue purifying apparatus which is different from the first, second, third and fourth embodiments in that: the adsorption device includes an adsorption sheet 44, and the adsorption sheet 44 is used for adsorbing grease in adipose tissue. The adsorption sheets 44 are provided in one or more, preferably more, than one, adsorption tube 43, or more, than one, or more, preferably more, than one, or more, adsorption sheets 44 have a larger contact area with the adipose tissue, which is more advantageous for adsorption of the fat. Moreover, the gap between the adjacent two adsorption sheets 44 can facilitate the operator to observe the fat state inside the working chamber 11.

Both ends of the adsorption sheet 44 are mounted in the working chamber 11 through the fixing members 5. The width of the adsorption sheets 44 is not greater than the radius of the fixing member 5 so that a plurality of adsorption sheets 44 are circumferentially arranged along the axis of the working chamber 11 as a center. Referring to fig. 17, the fixing member 5 is provided with a plurality of adsorption sheet fixing cavities 57 (when one adsorption sheet 44 is provided, only one adsorption sheet fixing cavity 57 may be provided), the plurality of adsorption sheet fixing cavities 57 are provided in one-to-one correspondence with the plurality of adsorption sheets 44, and the adsorption sheets 44 are inserted into the adsorption sheet fixing cavities 57, so as to realize stable fixing of the adsorption sheets 44 in the working cavity 11.

Referring to fig. 17, a plurality of suction sheet fixing chambers 57 are circumferentially distributed with respect to the center of the fixing member 5, and one or more fat passages 51 are provided between two adjacent suction sheet fixing chambers 57 on the fixing member 5. Likewise, a first positioning structure 58 of a convex shape is provided on the outside of the mount 5.

The thickness of the absorbent sheet 44 may be between 1mm and 5mm, preferably between 2mm and 4 mm. The number of the adsorption sheets 44 may be between 2 and 12, preferably between 6 and 10.

Preferably, the adsorbent sheet 44 is a void-filling material, and further, may be hollow or porous. More preferably, the adsorption sheet 44 is made of a lipophilic and hydrophobic material, and the material of the adsorption sheet 44 is exemplified by one of polypropylene, polyurethane, polyethylene, polytetrafluoroethylene, polyester and other polymer materials.

Example six

The present embodiment provides an adipose tissue purifying apparatus, which is different from the first, second, third, fourth, and fifth embodiments in that: the adsorption device comprises an adsorption ball which can adsorb grease in adipose tissue. Further, the adsorption ball is fixedly arranged in the working cavity 11 or freely arranged in the working cavity 11. For example, the adsorbent spheres may be in a semi-fixed form, such as being immobilized on adsorbent or non-adsorbent functional fibers. The number of the adsorption balls is not limited, and depends on the purification requirement of fat, the volume of the working chamber 11, and the like.

As an example, the diameter of the adsorption balls is small compared to the size of the working chamber 11. The number of the adsorption balls can be at least one layer in the working cavity 11, and the maximum number of the adsorption balls cannot exceed 1/2 of the whole volume of the working cavity 11.

The ratio of the total surface area of the adsorption balls to the total volume of the adsorption balls increases as the diameter of the adsorption balls decreases, so, as an example, if the internal volume of the working chamber 11 is the same, the diameter of the adsorption balls may be set smaller to increase the contact area with the grease, so that the grease adsorption is more sufficient. Of course, the diameter of the adsorption ball may be set to be not different from the inner diameter of the working chamber 11, for example, the diameter of the adsorption ball is larger than 1/3 of the inner diameter of the working chamber 11 and smaller than 1/2 of the inner diameter of the working chamber 11, and the height of the working chamber 11 can be increased to increase the volume of the working chamber 11, so that the working chamber 11 has enough space for purifying fat.

Further, the diameter of the adsorption ball needs to be larger than the aperture of the first filtering hole 21 of the first filtering device 2, so that the adsorption ball is prevented from penetrating out of the first filtering hole 21; preferably, the diameter of the adsorption balls is at least 1.5 times larger than the diameter of the first filter holes 21.

Preferably, the adsorption balls are materials filled with gaps, and further, hollow fibers or porous structures can be used. More preferably, the adsorption balls are made of oleophilic and hydrophobic materials, and the material of the adsorption balls is exemplified by one of polypropylene, polyurethane, polyethylene, polytetrafluoroethylene, polyester and other high polymer materials.

Example seven

The present embodiment provides an adipose tissue purifying apparatus, which is different from the above embodiments in that: the adsorption device is a combination of at least two of adsorption fibers 41, adsorption columns 42, adsorption tubes 43, adsorption sheets 44, and adsorption balls.

Illustratively, one of the combinations is exemplified: the adsorption device may include an adsorption column 42, adsorption fibers 41, and adsorption balls. The adsorption fiber 41 is arranged in the space between the outer wall of the adsorption column 42 and the inner wall of the working chamber 11, and the adsorption ball is fixed on the adsorption column 42, or fixed on the inner wall of the working chamber 11, or freely arranged in the space between the outer wall of the adsorption column 42 and the inner wall of the working chamber 11. Other combinations are not listed here.

Example eight

As shown in fig. 18 and 19, the present embodiment provides an adipose tissue purifying apparatus, which is different from the second embodiment in that: the second filtering device 3 and the third filtering device 6 are of an integrated structure and are positioned on the same plane, the second filtering device 3 and the third filtering device 6 are separated by the separation plate 64, the separation plate 64 is in sealing contact with the inner wall of the closed container 1, the separation plate 64 can allow grease to pass through and block fat tissue from passing through, the liquid outlet 141 and the fat outlet 142 are opposite to the second filtering device 3, and the grease outlet 143 is opposite to the third filtering device 6.

That is, the partition plate 64 is provided with the same third filter holes 61 as the third filter device 6, which allow only the grease to pass therethrough and not the fat to pass therethrough. After the adipose tissue in the working chamber 11 is cut by the second filtering device 3, the adipose tissue is collected through the adipose outlet 142, and the adipose tissue cannot pass through the partition plate 64 into the space above the partition wall third filtering device 6 due to the partition plate 64. While the grease on the side of the second filtering means 3 can pass through the partition plate 64 to be collected on the side of the third filtering means 6 so as to be discharged through the grease outlet 143.

Since the second filter 3 and the third filter 6 are integrally provided, a large installation space is not required, and thus the volume of the outlet end of the closed vessel 1 can be reduced, and the manufacturing cost can be reduced.

Example nine

As shown in fig. 20 and 21, the present embodiment provides an adipose tissue purifying apparatus, which is different from all of the above embodiments in that:

the inlet end of the closed container 1 is provided with a first three-way structure (i.e. arranged on the inlet end cover 13), the liquid inlet 131 is a first inlet of the first three-way structure, the fat inlet 132 is a second inlet of the first three-way structure, and an outlet of the first three-way structure is communicated with the working cavity 11. The outlet end of the closed container 1 is provided with a second three-way structure (i.e. arranged on the outlet end cover 14), the inlet of the second three-way structure is communicated with the working cavity 11, the liquid outlet 141 is a first outlet of the second three-way structure, and the fat outlet 142 is a second outlet of the second three-way structure. That is, the liquid inlet 131 and the fat inlet 132 are combined into one tee joint, and the liquid outlet 141 and the fat outlet 142 are combined into one tee joint, so that the manufacturing cost can be reduced, and meanwhile, the fat inlet 132 and the fat outlet 142 can be washed by flushing when the cleaning liquid is introduced and discharged due to the fact that the fat inlet 132 is communicated with the liquid inlet 131 and the fat outlet 142 is communicated with the liquid outlet 141, so that residual impurities are avoided.

Examples ten

As shown in fig. 22 and 23, the present embodiment provides an adipose tissue purifying apparatus, which is different from all of the above embodiments in that:

as shown in fig. 22 and 23, the adipose tissue purifying device provided in the present embodiment includes the third filtering device 6 and the grease outlet 143, and the third filtering device 6 and the grease outlet 143 are disposed outside the working chamber 11. Specifically, referring to fig. 24, 25 and 26, the third filtering device 6 is disposed on the grease outlet 143 to form an oil outlet structure. The fat outlet 143 is detachably connected to the fat outlet 142. After the fat is cleaned, the fat outlet 143 is abutted to the fat outlet 142 before the fat is extracted, and the aperture of the third filter device 6 only allows the fat to pass therethrough while blocking the fat from passing therethrough, so that the remaining excessive fat (i.e., the fat not adsorbed by the adsorption device) can be discharged through the third filter device 6 and the fat outlet 143. After the grease is discharged, the grease outlet 143 is detached from the fat outlet 142, the entire grease discharging structure is removed, and then the purified fat can be discharged through the fat outlet 142.

Referring to fig. 24, 25 and 26, the third filtering apparatus 6 and the grease outlet 143 are integrally formed, and the structure is connected to the grease outlet 142 through a luer lock, so that the disassembly and assembly are facilitated.

Optionally, the third filtering device 6 is disc-shaped, and is coaxially arranged with the grease outlet 143, and a filtering element 65 is embedded in the third filtering device 6, and the filtering element 65 is a filtering plate or a filtering membrane, and referring to fig. 27, the filtering element 65 is provided with a third filtering hole 61, and the size of the third filtering hole 61 should be smaller than that of the fat cell, so that the fat can be blocked from passing through.

Referring to fig. 24, one end of the grease outlet 143 is inserted into the fat outlet 142, and when the third filter device 6 abuts against the upper end surface of the fat outlet 142, it is indicated that the grease outlet 143 and the oil outlet structure formed by the third filter device 6 are mounted in place.

Preferably, the fat outlet 142 is provided with a check valve 8 and the fat outlet 143 is also provided with a check valve 8.

Example eleven

The present embodiment provides an adipose tissue purifying apparatus which is improved on the basis of the first embodiment. The fat tissue purifying device provided in this embodiment is provided with only the adsorption device for removing the fat and the third filtering device 6 for filtering the fat is not provided.

Referring to fig. 28 and 29, as a first possible implementation of the present embodiment, the adsorption device is an adsorption column 42. The advantages of using the adsorption column 42 and the fixing manner of the adsorption column 42 in the working chamber 11 are as described in the third embodiment, and will not be described here.

Referring to fig. 30 and 31, as a second possible implementation of the present embodiment, the adsorption apparatus is an adsorption tube 43, and the adsorption tube 43 includes at least two oppositely disposed arc-shaped fins 431. The advantages of using the adsorption tube 43 and the fixing manner of the adsorption tube 43 in the working chamber 11 are as described in the fourth embodiment, and will not be described here.

Referring to fig. 32 and 33, as a third possible implementation of the present embodiment, the adsorption device is an adsorption sheet 44, and one or more adsorption sheets 44 are provided. The advantages of using the adsorption sheet 44 and the fixing manner of the adsorption sheet 44 in the working chamber 11 are as described in the fifth embodiment, and will not be described here.

As a fourth implementation solution of this embodiment, the adsorption device is an adsorption ball, and the adsorption ball may be fixed in the working chamber 11 or freely disposed in the working chamber 11, as in the sixth embodiment, which is not described herein.

As a fifth possible embodiment of the present embodiment, the adsorption device is a combination of any two, any three, or more of the adsorption fibers 41, the adsorption column 42, the adsorption tube 43, the adsorption sheet 44, and the adsorption balls.

Example twelve

Based on the above and the same concept, as shown in fig. 34, an embodiment of the present invention also provides an adipose tissue purification method, which is applied to the adipose tissue purification device provided in any one of the embodiments described above, the adipose tissue purification method comprising the steps of:

S1, liquid injection and exhaust: the clean cleaning liquid is filled into the working chamber 11 of the closed container 1 through the liquid inlet 131 to discharge air in the working chamber 11.

As shown in fig. 35, a schematic diagram of the liquid injection and exhaust is shown. In step S1, the liquid inlet 131 is at the bottom end and the liquid outlet 141 is at the top end during liquid injection and gas discharge. The cleaning solution is introduced into the working chamber 11 of the closed container 1 through the liquid inlet 131 and gradually rises to the position of the liquid outlet 141 so as to discharge the gas in the working chamber 11 through the liquid outlet 141.

S2, injecting unrefined adipose tissue: unrefined adipose tissue is injected into working chamber 11 through fat inlet 132.

In step S2, when the unrefined adipose tissue is injected, the adipose tissue purifying device is inverted so that the adipose inlet 132 is at the top. As shown in fig. 36, which is a schematic view of injecting the unrefined adipose tissue, the fat inlet 132 is located at the top end, and the liquid outlet 141 and the fat outlet 142 are located at the bottom end. The fat tissue is cut up by the first filtering device 2 and enters between the first filtering device 2 and the second filtering device 3, the density of the fat tissue is smaller than that of the cleaning fluid, the fat tissue floats on the upper layer of the cleaning fluid after being injected, and part of the cleaning fluid is discharged from the liquid outlet 141 at the bottom end while the fat tissue is injected.

S3, sucking out grease: by shaking the closed vessel 1 to mix the adipose tissue and the washing liquid, the adsorption means adsorbs the grease during the period.

As shown in fig. 37, the fat tissue and the cleansing liquid are mixed by rotating and shaking, so that the cleansing liquid brings out the blood, anesthetic, interstitial fluid, and the like mixed in the fat tissue, and the grease is sufficiently contacted with the adsorbing device to suck the grease from the fat tissue.

S4, washing fat: the mixture is left to stand to delaminate the adipose tissue and the washing liquid containing impurities, the washing liquid containing impurities is discharged through the liquid outlet 141, and the clean washing liquid is injected through the liquid inlet 131.

As shown in fig. 38, a schematic diagram of discharging the cleaning liquid containing impurities is shown. The cleaning liquid containing impurities is discharged from the liquid outlet 141 at the bottom end, and the clean cleaning liquid is injected from the liquid inlet 131 at the top end.

S5, repeating the steps S3 and S4 until the discharged cleaning liquid becomes clear.

S60: the residual grease is discharged through the grease outlet 143.

As shown in fig. 39, a schematic diagram of the residual grease discharge is shown. After passing through the third filtering device 6, the residual grease is discharged through the grease outlet 143, and the third filtering device 6 can block the adipose tissues and prevent the adipose tissues from being discharged along with the grease.

Preferably, upon draining the fat, the fat refining device is turned over again such that the fat outlet 143 is at the top end and the liquid inlet 131 is at the bottom end. The injection of liquid into the working chamber 11 through the liquid inlet 131 and/or the fat inlet 132 is pressurized (preferably through the liquid inlet 131) so that the fat is discharged through the fat outlet 143.

S6, collecting fat: purified adipose tissue is collected through the adipose outlet 142.

As shown in fig. 40, a schematic diagram of the collection of purified fat is shown. After passing through the second filtering means 3, the fat is discharged via a fat outlet 142. At this time, the fat outlet 142 is at the top end, and the liquid inlet 131 and the fat inlet 132 are at the bottom end. The working chamber 11 is pressurized by injecting liquid through the fat inlet 132 and/or the liquid inlet 131 (preferably, by injecting liquid through the liquid inlet 131) to discharge the upper adipose tissues.

Preferably, the cleaning solution used is physiological saline. Compared with the pure water, the density of the normal saline is higher, so that the adipose tissues stably float on the upper layer of the normal saline. In addition, the physiological saline is contained in a medical infusion bag with a large volume, and the placement position is higher than that of the fat purification device, so that the supply is ensured to be sufficient in the whole purification process.

In step S60 and step S6, the liquid injected when the fat and fat tissues are discharged is also normal saline, and the normal saline in the medical infusion bag may be continuously injected.

Preferably, a section of hose is connected to the liquid inlet 131 and the liquid outlet 141, and the hose is convenient for connecting a container for containing the original cleaning liquid and a container for containing the impurity cleaning liquid. Namely, the liquid inlet 131 is connected with the medical infusion bag containing physiological saline through a hose.

Example thirteen

As shown in fig. 41, 42, 43 and 44, the present embodiment provides an adipose tissue purifying apparatus including a closed vessel 1, a second filtering device 3 and a third filtering device 6.

The closed container 1 defines a working chamber 11, the closed container 1 comprises an inlet end and an outlet end, the inlet end is provided with a liquid inlet 131 and a fat inlet 132 which are communicated with the working chamber 11, the outlet end is provided with a liquid outlet 141, a fat outlet 142 and a grease outlet 143 which are communicated with the working chamber, and the liquid inlet 141 and the liquid outlet 142 are both used for passing cleaning liquid. The second filtering means 3 is arranged in the working chamber 11 at the outlet end, through which second filtering means 3 and fat outlet 142 fat tissue can be discharged, the second filtering means 3 being arranged to cut the fat tissue to define the grain size of the extracted fat tissue. That is, when collecting the final purified fat, the fat tissue is discharged from the fat outlet 142 through the second filtering device 3, and the second filtering device 3 serves to reduce the size of the fat tissue and to limit the collection of the fat tissue of a certain size. The third filtering device 6 is disposed in the working chamber 11 or outside the working chamber 11, the third filtering device 6 is used for allowing grease to pass through and blocking fat tissue from passing through, and the grease outlet 143 is used for discharging grease filtered by the third filtering device 6. That is, in the present embodiment, only the third filter device 6 for discharging the grease may be provided, and the adsorbing device for adsorbing the grease may not be provided.

The fat tissue just extracted enters the closed container 1 through the fat inlet 132, and the cleaning liquid is injected into the working chamber 11 through the liquid inlet 131, so that the blood, anesthetic liquid, interstitial fluid and the like mixed with the whole fat particles can be removed after the cleaning liquid is fully mixed with the fat tissue. After the cleaning is finished, standing is carried out to separate the grease, the adipose tissues and the clarified cleaning liquid, then discharging the grease on the upper layer, and discharging the grease through the grease outlet 143 after passing through the third filtering device 6, wherein during the period, the third filtering device 6 only allows the grease to pass through so as to block the adipose tissues, so that the adipose tissues can not be carried out in the process of discharging the grease, and the reduction of the purification rate of the adipose tissues is avoided. After the completion of the fat discharge, the purified adipose tissue was collected through the fat outlet 142.

Referring to fig. 41, as a first possible implementation of this embodiment, the third filtering device 6 is disposed in the working chamber 11 and is located at the outlet end. The liquid inlet 131 and the fat inlet 132 are independently provided, and the liquid outlet 141 and the fat outlet 142 are independently provided.

The structure of the third filter device 6 is as described in the second embodiment. Specifically, referring to fig. 9, the third filter device 6 is provided with a third filter hole 61, and the third filter hole 61 has a smaller size than the second filter hole 31, which allows only grease to pass therethrough and does not allow fat to pass therethrough. The third filter hole 61 should have a size smaller than that of fat cells, thereby blocking the passage of fat. Preferably, the size of the third filter holes 61 is not more than 10 μm, more preferably, the size of the third filter holes 61 is not more than 5 μm.

Referring to fig. 42, as a second possible implementation of the present embodiment, the structure of the third filtering device 6 is as described in embodiment eight. Specifically, referring to fig. 19, the second filtering device 3 and the third filtering device 6 are in an integral structure and are located on the same plane, the second filtering device 3 and the third filtering device 6 are separated by a separation plate 64, the separation plate 64 is in sealing contact with the inner wall of the closed container 1, the separation plate 64 can allow grease to pass through and block fat tissue from passing through, the liquid outlet 141 and the fat outlet 142 are opposite to the second filtering device 3, and the grease outlet 143 is opposite to the third filtering device 6.

Referring to fig. 43, as a third possible implementation of the present embodiment, the third filtering device 6 may be identical to the first implementation described above. In this embodiment, the liquid inlet 131 and the fat inlet 132 are combined into a tee, and the liquid outlet 141 and the fat outlet 142 are combined into a tee, as described in the ninth embodiment. Specifically, the inlet end of the closed container 1 is provided with a first three-way structure (i.e. on the inlet end cover 13), the liquid inlet 131 is a first inlet of the first three-way structure, the fat inlet 132 is a second inlet of the first three-way structure, and the outlet of the first three-way structure is communicated with the working chamber 11. The outlet end of the closed container 1 is provided with a second three-way structure (i.e. arranged on the outlet end cover 14), the inlet of the second three-way structure is communicated with the working cavity 11, the liquid outlet 141 is a first outlet of the second three-way structure, and the fat outlet 142 is a second outlet of the second three-way structure. That is, the liquid inlet 131 and the fat inlet 132 are combined into one tee joint, and the liquid outlet 141 and the fat outlet 142 are combined into one tee joint, so that the manufacturing cost can be reduced, and meanwhile, the fat inlet 132 and the fat outlet 142 can be washed by flushing when the cleaning liquid is introduced and discharged due to the fact that the fat inlet 132 is communicated with the liquid inlet 131 and the fat outlet 142 is communicated with the liquid outlet 141, so that residual impurities are avoided.

Referring to fig. 44, as a fourth possible implementation of this embodiment, the third filtering device 6 is disposed outside the working chamber, and forms an oil outlet structure with the grease outlet 143, and its specific structure is as described in embodiment ten. Specifically, the grease outlet 143 is detachably connected to the fat outlet 142. Before extracting fat, the fat outlet 143 is abutted at the position of the fat outlet 142, and the aperture of the third filter device 6 allows only the fat to pass therethrough while blocking the fat from passing therethrough, so that the fat can be discharged through the third filter device 6 and the fat outlet 143. After the grease is discharged, the grease outlet 143 is detached from the fat outlet 142, the entire grease discharging structure is removed, and then the purified fat can be discharged through the fat outlet 142.

Referring to fig. 44, one end of the grease outlet 143 is inserted into the fat outlet 142, and when the third filter device 6 abuts against the upper end surface of the fat outlet 142, it is indicated that the grease outlet 143 and the oil outlet structure formed by the third filter device 6 are mounted in place.

Optionally, the adipose tissue purifying device further comprises a first filtering device 2, the first filtering device 2 is disposed in the working chamber 11 and is located at the inlet end, and the filtering holes of the second filtering device 3 are smaller than those of the first filtering device 2.

The space between the first filter device 2 and the second filter device 3 is defined as a receiving cavity, the freshly extracted adipose tissue must pass through the first filter device 2 to enter the receiving cavity, the first filter device 2 is used for preventing oversized fibrous tissue from entering the receiving cavity, and the space serves to chop the adipose tissue, so that the particles of the adipose tissue become smaller. That is, the fat introduced through the fat inlet is primarily filtered through the first filtering device 2 to chop the fat while intercepting a portion of the fibrous tissue, and the primarily filtered fat is introduced between the first filtering device 2 and the second filtering device 3. The adipose tissue is further minced by the second filtering means 3 to obtain fat particles of a desired size.

Alternatively, referring to fig. 41, the liquid inlet 131 and the liquid outlet 141 are each provided with an adjusting device 7 for adjusting the flow rate. The adjusting means 7 may be an adjusting valve, such as a ball valve, a plug valve, etc.; alternatively, the adjusting device 7 may be a clip, by which the flow adjustment at the liquid inlet 131 and the liquid outlet 141 may also be achieved.

Referring to fig. 41, the closed container 1 includes a cylinder 12 with two open ends, a working chamber 11 is disposed inside the cylinder 12, two ends of the cylinder 12 are respectively provided with an inlet end cover 13 and an outlet end cover 14, a liquid inlet 131 and a fat inlet 132 are both disposed on the inlet end cover 13, and a liquid outlet 141 and a fat outlet 142 are both disposed on the outlet end cover 14. Preferably, both the inlet end cap 13 and the outlet end cap 14 are removably attached (e.g., threaded) to the end of the cartridge 12 to facilitate installation and replacement of the first filter device 2 and the second filter device 3 therein.

Optionally, the adipose tissue purifying device further comprises an adsorption device disposed in the working chamber 11, the adsorption device is used for adsorbing the grease in the adipose tissue, and the adipose tissue adsorbed by the adsorption device is discharged through the second filtering device 3 and the fat outlet 142. The adsorption device can absorb grease in the process of cleaning the fat, so that the grease removal efficiency is improved. The specific structure of the adsorption device is as described in embodiment one to embodiment seven. It can be understood that, on the basis of the present embodiment, the combination of the various technical solutions formed by the embodiments with the adsorption device is all within the protection scope of the present invention.

Optionally, the adsorption means are located between the first filter means 2 and the second filter means 3.

Examples fourteen

The present embodiment provides an adipose tissue purifying apparatus which is improved on the basis of any of the above embodiments.

In this embodiment, a stirring device is disposed in the working chamber 11, and is used for stirring adipose tissue, promoting the mixing of adipose tissue and cleaning solution, and emulsifying oil. The stirring device can be a stirring ball, a stirring paddle and the like.

Example fifteen

Based on the same invention and concept as the thirteenth embodiment, the present embodiment provides an adipose tissue purification method applied to the adipose tissue purification device as described in the fourteenth embodiment, the adipose tissue purification method comprising the steps of:

w1, liquid injection and exhaust: filling the working chamber 11 of the closed container 1 with clean cleaning liquid through the liquid inlet 131 to discharge air in the working chamber 11; reference is made specifically to step S1 in embodiment thirteenth.

W2, injection into unrefined adipose tissue: injecting unrefined adipose tissue into working chamber 11 through fat inlet 132; reference is made specifically to step S2 in embodiment thirteenth.

W3, rinse fat: after shaking, the mixture is left to stand to delaminate the adipose tissues and the impurity-containing washing liquid, the impurity-containing washing liquid is discharged through the liquid outlet 141, and the clean washing liquid is injected through the liquid inlet 131. The cleaning liquid is brought into sufficient contact with the adipose tissue by shaking the closed vessel 1, and blood, anesthetic, interstitial fluid, and the like mixed in the adipose tissue are carried out. The cleaning liquid containing impurities is discharged from the liquid outlet 141 at the bottom end, and the clean cleaning liquid is injected from the liquid inlet 131 at the top end.

And W4, repeating the step W3 until the discharged cleaning liquid becomes clear.

W5, discharging grease: the grease filtered through the third filtering device 6 is discharged through the grease outlet 143; specifically referring to step S60 in the thirteenth embodiment.

W6, collecting fat: purified adipose tissue is collected through the adipose outlet 142. Reference is made specifically to step S6 in embodiment thirteenth.

In the various embodiments of the application, if there is no specific description or logical conflict, terms and/or descriptions between the various embodiments are consistent and may reference each other, and features of the various embodiments may be combined to form new embodiments according to their inherent logical relationships.

It is to be understood that the above examples of the present invention are provided for clarity of illustration only and are not limiting of the embodiments of the present invention. Various obvious changes, rearrangements and substitutions can be made by those skilled in the art without departing from the scope of the invention. It is not necessary here nor is it exhaustive of all embodiments. Any modification, equivalent replacement, improvement, etc. which come within the spirit and principles of the invention are desired to be protected by the following claims.

Claims

1. Adipose tissue purification device, characterized in that it comprises:

a closed container (1) defining a working chamber (11), wherein the closed container (1) comprises an inlet end and an outlet end, the inlet end is provided with a liquid inlet (131) and a fat inlet (132) which are communicated with the working chamber (11), the outlet end is provided with a liquid outlet (141) and a fat outlet (142) which are communicated with the working chamber (11), and the liquid inlet (131) and the liquid outlet (141) are both used for passing cleaning liquid;

the second filtering device (3) is arranged in the working cavity (11) and is positioned at the outlet end;

and the adsorption device is arranged in the working cavity (11) and is used for adsorbing grease in adipose tissues, and the adipose tissues adsorbed by the adsorption device are discharged through the second filtering device (3) and the fat outlet (142).

2. The adipose tissue purification device according to claim 1, wherein,

the adsorption device is directly fixed in the working cavity (11); or alternatively, the process may be performed,

the adipose tissue purification device further comprises a fixing piece (5), the adsorption device is arranged in the working cavity (11) through the fixing piece (5), and a fat channel (51) for fat to pass through is arranged on the fixing piece (5); or alternatively, the process may be performed,

The adsorption device is freely arranged in the working cavity (11).

3. Adipose tissue purification device according to claim 2, characterized in that said adsorption means are mounted in said working chamber (11) by means of a fixing element (5);

the adsorption device comprises a plurality of adsorption fibers (41); the fixing piece (5) is provided with a fiber fixing hole (52), and the adsorption fiber (41) is fixedly arranged in the fiber fixing hole (52) in a penetrating way; and/or the fixing piece (5) comprises an annular frame (53), one or more fixing rods (54) are arranged in the annular frame (53), and the adsorption fibers (41) are connected to the fixing rods (54);

and/or the adsorption device comprises an adsorption column (42), wherein the adsorption column (42) is of a hollow structure or a solid structure; an adsorption column mounting cavity (55) is formed in the fixing piece (5), and the adsorption column (42) is inserted into the adsorption column mounting cavity (55);

and/or the adsorption device comprises an adsorption pipe (43), wherein the adsorption pipe (43) comprises two arc-shaped sheets (431) which are spaced and oppositely arranged; an arc piece installation cavity (56) is formed in the fixing piece (5), and the arc piece (431) is inserted into the arc piece installation cavity (56);

and/or the adsorption device comprises an adsorption sheet (44), wherein the adsorption sheet (44) is provided with one or a plurality of adsorption sheets are circumferentially distributed with the axis of the working cavity (11) as the center; one or more adsorption sheet fixing cavities (57) are formed in the fixing piece (5), and the adsorption sheets (44) are inserted into the adsorption sheet fixing cavities (57).

4. A fat tissue purifying device according to claim 3, wherein the fixing member (5) is provided with a first positioning structure (58), the inner wall of the working chamber (11) is provided with a second positioning structure, and the first positioning structure (58) and the second positioning structure are matched so that the fixing members (5) at both ends of the adsorbing device are completely opposite to each other.

5. Adipose tissue purification device according to claim 1, characterized in that the adsorption means comprise adsorption spheres, which are fixed in the working chamber (11) or freely arranged in the working chamber (11).

6. Adipose tissue purification device according to any one of claims 1-5, characterized in that it further comprises a third filtering means (6), said third filtering means (6) being arranged in said working chamber (11) and being located at said outlet end, said outlet end being further provided with a fat outlet (143), said third filtering means (6) being adapted to allow fat to pass while blocking adipose tissue from passing, said fat outlet (143) being adapted to drain fat filtered by said third filtering means (6).

7. Adipose tissue purification device according to claim 6, characterized in that said third filtering means (6) is located between said second filtering means (3) and said fat outlet (143), said third filtering means (6) being provided with a first through hole (62) and a second through hole (63), said liquid outlet (141) being in communication with said working chamber (11) through said first through hole (62), said fat outlet (142) being in communication with said working chamber (11) through said second through hole (63);

Or, second filter equipment (3) with third filter equipment (6) are integrated into one piece structure, and are located the coplanar, second filter equipment (3) with separate through division board (64) between third filter equipment (6), division board (64) with inner wall sealing contact of airtight container (1), division board (64) can allow grease to pass through and block adipose tissue and pass through, liquid outlet (141) with fat export (142) with second filter equipment (3) are just right, grease export (143) with third filter equipment (6) are just right.

8. The adipose tissue purification device according to any one of claims 1-5, characterized in that it further comprises a third filtering means (6) and a fat outlet (143), said third filtering means (6) being arranged on said fat outlet (143), said fat outlet (143) being detachably connected to said fat outlet (142), said third filtering means (6) being adapted to allow fat to pass through while blocking adipose tissue from passing through, said fat outlet (143) being adapted to expel fat filtered by said third filtering means (6).

9. Adipose tissue purification device according to any one of claims 1 to 5, characterized in that said inlet end is provided with a first three-way structure, said liquid inlet (131) being a first inlet of said first three-way structure, said fat inlet (132) being a second inlet of said first three-way structure, an outlet of said first three-way structure being in communication with said working chamber (11);

And/or, the outlet end is provided with a second tee structure, an inlet of the second tee structure is communicated with the working cavity (11), the liquid outlet (141) is a first outlet of the second tee structure, and the fat outlet (142) is a second outlet of the second tee structure.

10. Adipose tissue purification device according to any one of claims 1 to 5, characterized in that the liquid inlet (131) and the liquid outlet (141) are provided with adjusting means (7) for adjusting the flow;

and/or a check valve (8) is arranged at each of the fat inlet (132) and the fat outlet (142);

and/or, the fat inlet (132) and the fat outlet (142) are provided with luer locks.

11. Adipose tissue purification device according to any one of claims 1 to 5, characterized in that stirring means are provided in the working chamber (11).

12. The adipose tissue purification device of any one of claims 1-5, wherein the adsorption device is porous; and/or the adsorption device is made of oleophilic and hydrophobic materials.

13. Adipose tissue purification device according to any one of claims 1 to 5, characterized in that it further comprises a first filtering means (2), said first filtering means (2) being arranged in said working chamber (11) and being located at said inlet end, the filtering holes of said second filtering means (3) being smaller than the filtering holes of said first filtering means (2), said adsorbing means being located between said first filtering means (2) and said second filtering means (3).

14. Adipose tissue purification method, characterized in that it is applied to an adipose tissue purification device according to any one of claims 1 to 13, comprising the steps of:

s1, liquid injection and exhaust: filling the working cavity (11) of the closed container (1) with clean cleaning liquid through a liquid inlet (131) so as to discharge air in the working cavity (11);

s2, injecting unrefined adipose tissue: injecting unrefined adipose tissue into the working chamber (11) through a fat inlet (132);

s3, sucking out grease: by shaking the closed container (1) to mix the adipose tissue and the washing liquid, the adsorption means adsorbs the grease during the period;

s4, washing fat: standing to delaminate the adipose tissue and the impurity-containing washing liquid, discharging the impurity-containing washing liquid through a liquid outlet (141), and injecting the clean washing liquid through the liquid inlet (131);

s6, collecting fat: purified adipose tissue is collected through the adipose outlet (142).

15. The method of purifying adipose tissue according to claim 14, wherein after step S5, step S6 is preceded by step S60: residual grease is discharged through a grease outlet (143).

16. The method for purifying adipose tissue according to claim 15, wherein,

in step S1, the liquid inlet (131) is at the bottom end and the liquid outlet (141) is at the top end when the liquid is injected and exhausted;

and/or, in step S2, inverting the adipose tissue purification device so that the adipose inlet (132) is at the tip when injecting the unrefined adipose tissue;

and/or, in step S60 and step S6, the fat outlet (143) and the fat outlet (142) are at the top end;

and/or, in step S60 and step S6, injecting liquid into the working chamber (11) through the fat inlet (132) and/or the liquid inlet (131) for pressurizing, so as to discharge fat tissue and grease;

and/or, the cleaning solution is physiological saline.

17. Adipose tissue purification device, characterized in that it comprises:

a closed container (1) defining a working cavity (11), wherein the closed container (1) comprises an inlet end and an outlet end, the inlet end is provided with a liquid inlet (131) and a fat inlet (132) which are communicated with the working cavity (11), the outlet end is provided with a liquid outlet (141), a fat outlet (142) and a grease outlet (143) which are communicated with the working cavity (11), and the liquid inlet (131) and the liquid outlet (141) are both used for passing cleaning liquid;

A second filtering device (3) arranged in the working cavity (11) and positioned at the outlet end, and fat tissues can be discharged through the second filtering device (3) and the fat outlet (142);

the third filtering device (6) is arranged in the working cavity (11) or outside the working cavity (11), the third filtering device (6) is used for allowing grease to pass through and blocking fat tissues from passing through, and the grease outlet (143) is used for discharging the grease filtered by the third filtering device (6).