CN218089620U - Device for preparing tissue - Google Patents

Device for preparing tissue Download PDF

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Publication number
CN218089620U
CN218089620U CN202220882743.6U CN202220882743U CN218089620U CN 218089620 U CN218089620 U CN 218089620U CN 202220882743 U CN202220882743 U CN 202220882743U CN 218089620 U CN218089620 U CN 218089620U
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cavity
sleeve
discharge hole
preparing tissue
sleeve portion
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CN202220882743.6U
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范冬梅
崔鑫
张国强
董骧
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Beijing Naton Medical Technology Holdings Co Ltd
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Beijing Naton Medical Technology Holdings Co Ltd
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Abstract

The utility model discloses a device for preparing tissue, a device for preparing tissue includes body, at least one filter screen and collection sleeve, the body has first cavity, be equipped with filling hole and discharge hole on the body, the filter screen transversely sets up in the body, all constitute between the adjacent filter screen and between filter screen and the body top and hold the chamber, it is equipped with stirring element to hold the intracavity, it establishes at the lower extreme of body and along the length direction of body portable between primary importance and second place to collect the sleeve cover, it has the second cavity to collect the sleeve, at primary importance, collect the sleeve can open the discharge hole in order to communicate primary importance with the second cavity, at the second place, it can close the discharge hole in order to break off primary importance and second cavity to collect the sleeve. The utility model discloses a device for preparing tissue can improve effective active cell enrichment rate and separation efficiency, has reduced spare part quantity simultaneously, has simplified the preparation process, has reduced the pollution risk because of too much operating procedure brings.

Description

Device for preparing tissue
Technical Field
The utility model relates to the technical field of medical equipment, specifically, relate to a device for preparing tissue.
Background
The blood vessel matrix component contains a plurality of cells with repair function and cell groups formed by cell factor mixtures, has strong self-renewal, proliferation capacity and differentiation potential, can replace necrotic cells, can synthesize a plurality of bioactive factors through paracrine and autocrine, activates cells and blood vessel regeneration paths, plays a role in nutrition for cartilage, has a homing function, can actively migrate to a cartilage ischemia or damaged part for repair and reconstruction under the action of in-vivo microenvironment, and has immune suppression and anti-inflammatory effects.
However, the tissue preparation apparatus in the related art needs to include a large number of devices to satisfy the steps of cleaning, crushing, filtering, separating, extracting, etc. of the tissue, which results in a complex preparation process and low preparation efficiency, and the transfer of the tissue among the devices easily causes the contamination of the cell tissue, and the tissue preparation apparatus in the related art has low activity of the cell tissue prepared and low preparation efficiency.
SUMMERY OF THE UTILITY MODEL
The present invention aims at solving at least one of the technical problems in the related art to a certain extent.
Therefore, the embodiment of the utility model provides a device for preparing tissue, this device for preparing tissue can improve effective active cell enrichment ratio and separation efficiency, has reduced spare part quantity simultaneously, has simplified the preparation process, has greatly practiced thrift the time in the doctor's operation, has reduced the pollution risk because of too much operating procedure brings.
The utility model discloses a device for preparing tissue includes: the pipe body is provided with a first cavity, the top of the pipe body is provided with an injection hole, the bottom of the pipe body is provided with a discharge hole, and the injection hole and the discharge hole can be opened and closed; the filter screens are transversely arranged in the pipe body, accommodating cavities are formed between every two adjacent filter screens and between the filter screens and the top of the pipe body, and at least one stirring element is arranged in each accommodating cavity; a collecting sleeve sleeved at the lower end of the pipe body and movable along the length direction of the pipe body between a first position and a second position, the collecting sleeve having a second cavity, the collecting sleeve being capable of opening the discharge hole to communicate the first cavity with the second cavity at the first position, and closing the discharge hole to disconnect the first cavity from the second cavity at the second position.
The utility model discloses a device of preparation tissue, be equipped with at least one filter screen in the body, all hold stirring element between the adjacent filter screen and between filter screen and the body top, thereby can utilize the filter screen in time to separate the tissue granule of different particle sizes at the broken in-process of tissue, prevent that the tissue granule of little particle size and the tissue granule of big particle size from mixing together and lasting the striking and damaging the cell that exposes in the tissue granule, improve effective active cell enrichment multiplying power and separation efficiency, the first cavity of collecting telescopic second cavity and body can communicate and break off, then when preparing the tissue, can open the discharge hole with intercommunication second cavity and first cavity, treat to prepare and accomplish the back, can close the discharge hole with breaking off second cavity and first cavity, thereby store cell tissue to in the second cavity in order to supply the tissue to draw, and the cooperation is collected telescopic opening and closing, can wash tissue cell, therefore, the device of preparation tissue of this application can realize the preparation tissue simultaneously, wash and store and draw, preparation equipment and storage that need bring the independent setting in traditional preparation device and draw equipment, this application has reduced the preparation of preparation tissue and operation section doctor, greatly the operation process is used for a plurality of tissue pollution reduction together, the operation risk of preparation tissue is about the preparation.
In some embodiments, the filter screen is a plurality of filter screens, and a plurality of filter screens are arranged at intervals in the length direction of the pipe body, and the aperture of each filter screen is gradually reduced in the arrangement direction away from the top of the pipe body.
In some embodiments, the stirring elements may be provided in a spherical or polyhedral shape, and the diameters of the stirring elements in the plurality of receiving cavities are gradually reduced in a direction away from the top of the pipe body.
In some embodiments, the tube body includes a tube body, a centrifugal frame and a cover body, the centrifugal frame is located in the tube body and connected to the inner wall of the tube body, the filter screen is disposed in the centrifugal frame, the cover body is connected to the top of the tube body, the injection hole is disposed on the cover body, the injection hole is provided with an upper plug, and the discharge hole is disposed at the bottom of the tube body.
In some embodiments, the collecting sleeve includes a lower plug and a sleeve portion, the sleeve portion is movably sleeved on the lower end of the barrel, the lower plug is disposed on the bottom wall of the sleeve portion, and the lower plug can extend into or be out of the discharge hole along with the movement of the sleeve portion to realize the closing or opening of the discharge hole.
In some embodiments, the stirring element surface is provided with a spike-like protrusion structure.
In some embodiments, the collecting sleeve further includes a lower end cap and an extraction pad, the lower end cap is sleeved on the sleeve portion and is in threaded connection with the sleeve portion, a leak hole is formed in the bottom wall of the sleeve portion, a suction hole is formed in the bottom wall of the lower end cap and is opposite to the leak hole, and the extraction pad is clamped between the bottom wall of the sleeve portion and the bottom wall of the lower end cap.
In some embodiments, the leak hole is located at a central position of the bottom wall of the sleeve portion, and a connecting plate extending inward in a radial direction of the leak hole is provided at an edge of the leak hole, and the lower plug is connected to the connecting plate.
In some embodiments, the lower end of the cylinder has a tapered section having a sectional area gradually decreasing from top to bottom, and the bottom of the tapered section has the discharge hole.
In some embodiments, the device for preparing tissue further comprises a filtering membrane attached at the exit orifice.
Drawings
Fig. 1 is a schematic structural view of an apparatus for preparing tissue according to an embodiment of the present invention.
Fig. 2 is a schematic structural view of an apparatus for preparing tissue according to another embodiment of the present invention.
Fig. 3 is an exploded view of an apparatus for preparing tissue according to an embodiment of the present invention.
Fig. 4 is a schematic structural view of a stirring element of an apparatus for preparing tissue according to an embodiment of the present invention.
Fig. 5 is a schematic view of the stirring element of the device for preparing tissue according to another embodiment of the present invention.
Reference numerals:
the device comprises a pipe body 1, a barrel body 11, a centrifugal frame 12, a cover body 13, an upper plug 14, a filter screen 2, a collecting sleeve 3, a sleeve portion 31, a lower end cover 4, a lower plug 5 and a drawing pad 6.
Detailed Description
Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings. The embodiments described below with reference to the drawings are exemplary and intended to be used for explaining the present invention, and should not be construed as limiting the present invention.
As shown in fig. 1-3, the device for preparing tissue according to the embodiment of the present invention comprises a tubular body 1, at least one sieve 2 and a collecting sleeve 3. It should be noted that the device for preparing tissue of the present application can be directed to extracting effective cost such as stem cells or other effective components such as "blood vessel matrix component" in adipose tissue for applying to the existing clinical treatment technology for treating osteoarthritis, articular cartilage defect, cartilage injury and degeneration, chronic wound healing, medical and cosmetic filling and other diseases.
As shown in fig. 1 to 3, the tube body 1 has a first cavity, the tube body 1 extends in an up-and-down direction and the top of the tube body 1 is provided with an injection hole (not shown), and the bottom of the tube body 1 is provided with a discharge hole (not shown), and both the injection hole and the discharge hole can be opened and closed. It will be appreciated that when it is desired to inject, for example, adipose tissue into the tube body 1, the injection hole may be opened, and after extraction of the effective components in the adipose tissue is completed, the discharge hole may be opened to collect the effective components.
As shown in fig. 1-3, filter screen 2 transversely sets up in body 1, and filter screen 2 can be one or more, and a plurality of filter screens 2 are arranged at the length direction interval of body 1, and in the direction of arranging of keeping away from body 1 top, and a plurality of filter screens 2's aperture reduces gradually, all constitutes between the adjacent filter screen 2 and between filter screen 2 and the body 1 top and holds the chamber, holds the intracavity and is equipped with stirring element.
For convenience of understanding, taking adipose tissue as an example, the preparation process of the preparation device of the present application includes: adipose tissues are injected into the tube body 1 through the injection hole, and then the tube body 1 is shaken to make the stirring member in the first accommodating chamber hit the adipose tissues, so that the desired cells are sufficiently exposed, i.e., the adipose tissues are crushed to form fat particles.
Further, along with the proceeding of the impact, after the part in the fat particles is smashed to a small enough particle size, the small-particle size fat particles can pass through the filter screen 2 at the bottom of the first containing cavity, it should be noted that, if the small-particle size fat particles meet the preparation requirement at this time, the filter screen 2 does not need to be added, that is, the tissue only needs to be collided and separated in one containing cavity to meet the preparation requirement, and if the small-particle size fat particles falling from the first containing cavity cannot meet the requirement, the filter screen 2 can be added below the layer of filter screen 2 at a certain distance to form a second containing cavity, so that the small-particle size fat particles separated in the first containing cavity fall into the second containing cavity, the fat particles failing to meet the size are separated by the filter screen 2 and continue to be impacted in the first containing cavity, similarly, after the small-particle size fat particles fall into the second containing cavity, the stirring element in the containing cavity continues to impact the small-particle size fat particles to form the smaller-particle size fat particles, and accordingly, according to the preparation requirement, the filter screen 2 below the second containing cavity can continue to prepare the final quantity, and the final quantity is determined.
In other words, this application can set up one deck or multilayer filter screen 2 according to the collision demand of tissue, and the stirring component who holds between filter screen 2 can carry out layer upon layer striking, separation to the fat granule to the extraction of effective cell tissue in the final completion to adipose tissue.
The collecting sleeve 3 is sleeved at the lower end of the pipe body and is movable along the length direction of the pipe body 1 between a first position and a second position, the collecting sleeve 3 is provided with a second cavity, the discharging hole can be opened by the collecting sleeve 3 at the first position to communicate the first cavity with the second cavity, and the discharging hole can be closed by the collecting sleeve 3 at the second position to disconnect the first cavity from the second cavity.
Thus, the device for preparing tissue of the present application can be used as both a washing device and a storage and extraction device for tissue, for example, after adipose tissue is injected into the tube body 1, after centrifugation, active ingredients in the adipose tissue are deposited in the collection sleeve 3, the collection sleeve 3 is rotated to close the discharge hole to disconnect the first and second cavities, waste liquid in the tube body is poured out, if SVF is to be washed, physiological saline can be injected from the injection hole to switch the first and second cavities to a connected state, centrifugation is performed after the injection hole is blocked, and the above steps are repeated until the fat is washed clean.
Furthermore, adipose tissue is through stirring the component back of colliding layer upon layer, and the cell tissue that accords with the demand can be piled up in collecting sleeve 3, and when preparation completion or need to be taken, the extraction syringe needle can directly stretch into the second cavity and extract cell tissue.
The utility model discloses a device of preparation tissue, be equipped with at least one filter screen in the body, all hold stirring element between the adjacent filter screen and between filter screen and the body top, thereby can utilize the filter screen in time to separate the tissue granule of different particle diameters at the tissue crushing in-process, prevent that the tissue granule of little particle diameter and the tissue granule of big particle diameter from mixing together and lasting striking and damage the cell that exposes in the tissue granule, improve effective active cell enrichment rate and separation efficiency, the first cavity of collecting telescopic second cavity and body can feed through and break off, then when preparing the tissue, can open the discharge hole with intercommunication second cavity and first cavity, treat to prepare the completion back, can close the discharge hole with disconnection second cavity and first cavity, thereby store cell tissue to the second cavity in for the tissue draws, and the cooperation is collected telescopic opening and closing, can wash tissue cell, therefore, the device of preparation tissue of this application can realize the preparation of tissue simultaneously, wash and store and draw, compare in the preparation equipment that traditional preparation device need not independently set up and store the extraction equipment, this application has reduced the preparation of saving doctor, the operation is used for a plurality of tissue and has reduced the operation risk when the tissue is transferred together.
Alternatively, as shown in fig. 4 and 5, the stirring members may be provided in a spherical or polyhedral shape, and the diameter sizes of at least two of the stirring members in the plurality of stirring members in the same accommodating chamber may be the same or different. In other words, the size of the stirring elements in the same containing chamber may be different. Optionally, the surface of the stirring element is provided with a thorn-like projection structure, which facilitates the effective components to be fully exposed during the impact process.
Optionally, the impact beads within the plurality of receiving cavities taper in diameter in a direction away from the top of the tube body 1, thereby facilitating efficient impact of the tissue particles of progressively decreasing size.
Further, as shown in fig. 1-3, the tube 1 includes a barrel 11, a centrifugal frame 12 and a cover 13, the centrifugal frame 12 is located in the barrel 11 and connected to the inner wall of the barrel 11, the filter screen 2 is disposed in the centrifugal frame 12, the cover 13 is connected to the top of the barrel 11, the injection hole is disposed on the cover 13, the injection hole is disposed with an upper plug 14, and the discharge hole is disposed at the bottom of the barrel 11. It should be noted that, in consideration of the difficulty of disposing the filter screen 2 on the pipe wall, the filter screen 2 of the present application is integrated on the centrifugal frame 12, that is, the filter screen 2 and the centrifugal frame 12 are an integral structural member.
Further, as shown in fig. 1 to 3, the collecting sleeve 3 includes a sleeve portion 31, the sleeve portion 31 is disposed at the lower end of the cylinder 11 and is movable in the up-down direction relative to the sleeve portion 31, a lower plug 5 is disposed on the bottom wall of the sleeve portion 31, and the lower plug 5 can extend into or be removed from the discharge hole with the movement of the sleeve portion 31 to close or open the discharge hole. Therefore, the lower plug 5 opens the discharge hole to allow the cell components to pass into the sleeve portion 31, and the lower plug 5 closes the discharge hole to separate and lock the separated cell components in the sleeve portion 31, thereby facilitating the subsequent extraction.
Preferably, the sleeve portion 31 is threadedly connected with the barrel 11. Therefore, the sleeve part 31 is reliably connected with the cylinder body 11, is tightly attached, and can be driven by screwing the sleeve part 31 to move the lower plug 5 so as to open and close the discharge hole.
Further, as shown in fig. 1-3, the collecting sleeve 3 further includes a lower end cap 4 and an extraction pad 6, the lower end cap 4 is sleeved on the sleeve portion 31 and is in threaded connection with the sleeve portion 31, a leak hole is formed on the bottom wall of the sleeve portion 31, a suction hole is formed on the bottom wall of the lower end cap 4 and is opposite to the leak hole, and the extraction pad 6 is clamped between the bottom wall of the sleeve portion 31 and the bottom wall of the lower end cap 4.
Therefore, when the prepared active ingredients need to be extracted, the injector can be utilized to sequentially penetrate through the suction hole, the extraction pad 6 and the leak hole to extract the active ingredients in the sleeve part 31, so that the fluctuation of the liquid level in the extraction process can be avoided, the cell components are prevented from being exposed in the external environment due to extraction operation, and the sterile environment and the effective cell components in the tube body 1 are ensured not to be polluted.
Preferably, the leak hole is located at the central position of the bottom wall of the sleeve portion 31, and the edge of the leak hole is provided with a connecting plate extending inwards in the radial direction of the leak hole, and the lower plug 5 is connected with the connecting plate.
Preferably, the lower end of the cylinder 11 has a tapered section having a sectional area gradually decreasing from top to bottom, and the bottom of the tapered section has a discharge hole. The tapered section thus gradually contracts facilitates the flow of the cellular components along the inclined inner wall towards the discharge orifice.
Further, the apparatus for preparing tissue of the present application further comprises a filtering membrane connected to the discharge hole, the filtering membrane being capable of filtering debris generated during the tissue disruption process, preventing the debris from flowing into the sleeve portion 31 during the centrifugation process, and ensuring that the effective components inside the sleeve portion 31 are not contaminated.
In addition, the device for preparing the tissue can also be applied to the integrated cleaning and separation of the components of the vascular stroma prepared by the enzymolysis process.
Specifically, taking adipose tissue as an example, after the operation to extract fat, standing to remove swelling liquid, unscrewing the upper plug 14, injecting adipose tissue into the adipose tissue through an injection hole, injecting fat digestive enzyme, allowing the adipose tissue and the fat digestive enzyme to be fully mixed on a shaking table for incubation and digestion at constant temperature, after the reaction is completed, rotating the collecting sleeve 3 to open a discharge hole, after the tissue components are transferred into the collecting sleeve 3, rotating the collecting sleeve 3 to close the discharge hole, opening the cover body 13 to pour out the uppermost layer of adipose tissue and residual solution, then screwing back the cover body 13, opening the upper plug 14 and the collecting sleeve 3, injecting physiological saline into the collecting sleeve 3, centrifugally washing the cell components at low speed, closing the collecting sleeve 3 again, locking the cell components in the collecting sleeve 3, repeating the above steps in cooperation with the opening and closing of the collecting sleeve 3, removing the cleaning liquid and pouring the physiological saline again for cleaning, repeating the cleaning steps for several times to obtain pure cell components, and after closing the collecting sleeve 3, puncturing the reserved extraction pad 6 area with a syringe for extraction.
Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.
In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; may be mechanically coupled, may be electrically coupled or may be in communication with each other; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.
In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.
In the present disclosure, the terms "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present disclosure. In this specification, the schematic representations of the terms used above are not necessarily intended to refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Moreover, various embodiments or examples and features of various embodiments or examples described in this specification can be combined and combined by one skilled in the art without being mutually inconsistent.
Although embodiments of the present invention have been shown and described, it is understood that the above embodiments are exemplary and should not be construed as limiting the present invention, and that variations, modifications, substitutions and alterations can be made to the above embodiments by those of ordinary skill in the art without departing from the scope of the present invention.

Claims (10)

1. A device for preparing tissue, comprising:
a pipe body having a first cavity, the pipe body having a top provided with an injection hole and a bottom provided with a discharge hole, the injection hole and the discharge hole being openable and closable;
the filter screens are transversely arranged in the pipe body, accommodating cavities are formed between every two adjacent filter screens and between the filter screens and the top of the pipe body, and at least one stirring element is arranged in each accommodating cavity;
a collecting sleeve sleeved at the lower end of the pipe body and movable along the length direction of the pipe body between a first position and a second position, the collecting sleeve having a second cavity, the collecting sleeve being capable of opening the discharge hole to communicate the first cavity with the second cavity at the first position, and closing the discharge hole to disconnect the first cavity from the second cavity at the second position.
2. The device for preparing tissue according to claim 1, wherein the filter screen is a plurality of filter screens, the plurality of filter screens are arranged at intervals along the length of the tube body, and the aperture of each filter screen is gradually reduced in the arrangement direction away from the top of the tube body.
3. The device for preparing tissue according to claim 1, wherein the agitating elements are provided in a spherical or polyhedral shape, and the diameter of the agitating elements in the plurality of receiving cavities is gradually reduced in a direction away from the top of the tube body.
4. The apparatus for preparing tissue according to any one of claims 1 to 3, wherein the tube body comprises a barrel body, a centrifugal frame and a cover body, the centrifugal frame is positioned in the barrel body and connected with the inner wall of the barrel body, the filter screen is arranged in the centrifugal frame, the cover body is connected to the top of the barrel body, the injection hole is arranged on the cover body, the injection hole is provided with an upper plug, and the discharge hole is arranged at the bottom of the barrel body.
5. The device for preparing tissue according to claim 4, wherein the collecting sleeve includes a lower plug and a sleeve portion, the sleeve portion is movably fitted over a lower end of the barrel, the lower plug is provided on a bottom wall of the sleeve portion, and the lower plug is capable of being inserted into or removed from the discharge hole with the movement of the sleeve portion to effect the closing or opening of the discharge hole.
6. Device for preparing tissue according to claim 1, characterized in that the surface of the stirring element is provided with a structure of barbed protrusions.
7. The device for preparing tissue according to claim 5, wherein the collection sleeve further comprises a lower end cap and an extraction pad, the lower end cap is sleeved on the sleeve portion and is in threaded connection with the sleeve portion, a leak hole is formed in the bottom wall of the sleeve portion, a suction hole is formed in the bottom wall of the lower end cap, the leak hole is opposite to the suction hole, and the extraction pad is clamped between the bottom wall of the sleeve portion and the bottom wall of the lower end cap.
8. The device for preparing tissue according to claim 7, wherein the orifice is located at a central position of the bottom wall of the sleeve portion, and an edge of the orifice is provided with a connecting plate extending inward in a radial direction of the orifice, and the lower plug is connected to the connecting plate.
9. The device for preparing tissue according to claim 4, wherein the lower end of the cylinder has a tapered section having a sectional area gradually decreasing from top to bottom, and the bottom of the tapered section has the discharge hole.
10. The apparatus for preparing tissue according to claim 1, further comprising a filter membrane attached at the exit orifice.
CN202220882743.6U 2022-04-15 2022-04-15 Device for preparing tissue Active CN218089620U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202220882743.6U CN218089620U (en) 2022-04-15 2022-04-15 Device for preparing tissue

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202220882743.6U CN218089620U (en) 2022-04-15 2022-04-15 Device for preparing tissue

Publications (1)

Publication Number Publication Date
CN218089620U true CN218089620U (en) 2022-12-20

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Application Number Title Priority Date Filing Date
CN202220882743.6U Active CN218089620U (en) 2022-04-15 2022-04-15 Device for preparing tissue

Country Status (1)

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CN (1) CN218089620U (en)

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