CN218755728U

CN218755728U - Adipose tissue digestion and extraction adipose-derived stem cell device

Info

Publication number: CN218755728U
Application number: CN202222813346.6U
Authority: CN
Inventors: 卓鑫杰; 李旭昭; 姚玲玲
Original assignee: Huanshenghui Biogenetic Technology Beijing Co ltd
Current assignee: Huanshenghui Biogenetic Technology Beijing Co ltd
Priority date: 2022-10-25
Filing date: 2022-10-25
Publication date: 2023-03-28
Anticipated expiration: 2032-10-25

Abstract

The utility model discloses a device for digesting and extracting adipose-derived stem cells from adipose tissues, which comprises a separator, a digester and a separation purifier, wherein a sealing plate is arranged at the top of the separator, a first injector connector and a second injector connector are arranged on the sealing plate, a steel belt screen is arranged on the inner wall of the separator, and a communicating pipe communicated with the digester is arranged at the bottom of the separator; a switching valve assembly is arranged at the communication position of the digester and the separation purifier, a first cell screen is arranged in the digester and is positioned at the bottom of the communication pipe, a third injector connector is arranged at one end of the digester, and a waste liquid discharge pipe is arranged on the digester at the bottom of the first cell screen; the internally mounted of separation and purification ware has second cell screen cloth and aseptic polyethylene filter membrane, and separation and purification ware one end is installed the fourth syringe connector, overcomes current fat particle purification device inefficiency, separation effect subalternation problem to furthest improves fat stem cell's separation yield and cultivation efficiency.

Description

Adipose tissue digestion and extraction adipose-derived stem cell device

Technical Field

The utility model relates to an adipose tissue digestion extracts adipose stem cell device, concretely relates to adipose tissue extraction technical field.

Background

The adipose-derived stem cells are stem cells with the potential of multi-directional differentiation separated from adipose tissues in recent years, and researches show that the stem cells can be stably proliferated in vitro and have low apoptosis rate, and meanwhile, the adipose-derived stem cells have the advantages of easiness in material obtaining, capability of obtaining a large amount of stem cells from a small amount of tissues, suitability for large-scale culture, small damage to organisms and the like.

In practice, the fat particles obtained by suction from the human body are often doped with impurities such as small coagulated blood clots, fat fragments, fibrous tissue masses, tumescent fluid and the like, and the impurities can damage normal tissues, inhibit the activity of the fat particles and reduce the purity of the culture of the adipose-derived stem cells. Only by the method of saline washing, the oil drops broken by the mature fat are difficult to wash clean, and the damage and death of fat cells are increased. In the field of cell culture, the method overcomes the defect that the cell suspension is plated and cultured in the last step in the process of separating and purifying the adipose-derived stem cells, and the cell sticking rate is not high in the process, so that the cell purification yield is low and the production requirement cannot be met.

Therefore, it is necessary to invent a device for extracting adipose-derived stem cells by adipose tissue digestion to solve the above problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at providing an adipose tissue digestion draws fat stem cell device lies in overcoming current fat particle purification device inefficiency, separation effect subalternation problem, but the method of fat in the fat separation purification in-process efficient removal fat stem cell separation purification to the at utmost improves fat stem cell's separation yield and cultivation efficiency, with the above-mentioned weak point in the solution technology.

In order to achieve the above object, the present invention provides the following technical solutions:

a device for digesting and extracting adipose-derived stem cells from adipose tissues comprises a separator, a digester and a separation purifier, wherein the separator is of a central structure with an opening at the upper end, a sealing plate is arranged at the top of the separator, a first injector connector and a second injector connector which are communicated with the inside of the separator are arranged on the sealing plate, a steel bar screen is arranged on the inner wall of the separator, and a communicating pipe communicated with the digester is arranged at the bottom of the separator; the digester and the separation purifier are both of a central structure and are communicated, a switch valve assembly is installed at the communication position of the digester and the separation purifier, a first cell screen is installed inside the digester and is located at the bottom of the communication pipe, a third injector connector is installed at one end, away from the separation purifier, of the digester, a waste liquid discharge pipe is installed on the digester at the bottom of the first cell screen, and a valve is installed on the waste liquid discharge pipe; the separation purifier is internally provided with a second cell screen and a sterile polyethylene filter membrane, and a fourth syringe connector is arranged at the center of one end of the separation purifier, which is far away from the digester.

Preferably, first cell screen is hollow hemispherical structure, just the aperture of first cell screen is 400um, the aperture of second cell screen is 100um.

Preferably, the sealing plate is of an inverted convex structure, the diameter of the bottom of the sealing plate is the same as the inner diameter of the separator, and a sealing rubber ring is sleeved on the outer side of the bottom of the sealing plate.

Preferably, both ends of the communicating pipe are of threaded structures, and the communicating pipe is connected with the separator and the digester in an internal and external threaded mode.

Preferably, the switch valve subassembly is including the disk seat, the disk seat is connected near one end each other with digester and separation and purification ware, install the pivot in the disk seat, the dwang is installed to the bottom of disk seat, the dwang is connected with the pivot, install the valve dish in the pivot.

Preferably, one end of the digester and one end of the separation purifier, which are far away from each other, are provided with a breaking part, the breaking parts of the digester and the separation purifier are provided with flanges, and the breaking parts of the digester and the separation purifier are connected with a plurality of fixing bolts through two flanges.

Preferably, the support frame subassembly is all installed in the outside of separator and digester and separation purifier, the support frame subassembly is including the lantern ring, the support column is installed to the bottom of lantern ring, the base plate is installed to the bottom of support column, the support column of the support frame subassembly in the separator outside is L type structure.

In the technical scheme, the utility model provides a technological effect and advantage:

1. the first syringe connector of the utility model is connected with a syringe with fat outside so that the pumped fat enters the upper part of the inside of the separator; the second syringe connector is connected with external normal saline so that the normal saline enters the separator and washes fat in the separator; and in the process of rinsing again, the fat mixed liquid passes through a steel bar screen on the inner wall of the separator, the fat tissue sample to be treated is sprinkled through the steel bar screen, and the cord-shaped tissue in the fat tissue mixed liquid is hung on the filaments of the steel bar screen, wherein the cord-shaped tissue comprises nerves, blood vessels, fibers and fascia. The fat particles are fully separated in the water flow flushing, oil drops, swelling liquid and fine fat fragments in the fat tissue sample to be treated are flushed to a digester, small pieces of fat tissue are left in a first cell screen after passing through the first cell screen, and other waste liquid is discharged from a waste liquid discharge pipe of the digester; not only can remove substances such as anesthetics, blood, oil drops, moisture and the like, but also can efficiently separate oil drops released by broken cells from fat;

2. the utility model discloses a two section structures, get rid of fibrous tissue stirring separation and grease filtration process and combine to set up to a body structure, can be under the totally enclosed state, fibrous tissue, the broken oil drop of ripe cell, swelling liquid in the fat mixed liquid are dispelled in non-centrifugal separation. And the lipophilic characteristic of the polyethylene surface is utilized to increase the corresponding sterile polyethylene filter membrane; the fat cells after digestion pass through the separation purifier, so that the grease floating on the liquid surface is attached to the surface of the sterile polyethylene filter membrane, the grease separation rate and the culture efficiency of the fat stem cells can be improved to the maximum extent by using the device, and the fat purification operation is simpler and faster than the operation executed according to the prior art.

Drawings

In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments described in the present invention, and other drawings can be obtained by those skilled in the art according to these drawings.

FIG. 1 is a perspective view of the overall structure of the present invention;

FIG. 2 is a perspective view of the present invention, shown in whole (with the stand assembly removed) and disassembled;

FIG. 3 is a cross-sectional perspective view of the entire (support frame assembly removed) embodiment of the present invention;

fig. 4 is a perspective view of the switching valve assembly of the present invention;

fig. 5 is a perspective view of the supporting frame assembly of the present invention.

Description of reference numerals:

1. a separator; 2. a digester; 3. a separation purifier; 4. a first syringe connector; 5. a second syringe connector; 6. sealing plates; 7. a steel bar screen; 8. a communicating pipe; 9. a third syringe connector; 10. a first cell screen; 11. a waste liquid discharge pipe; 12. a switch valve assembly; 13. a second cell screen; 14. sterile polyethylene filter membranes; 15. a fourth syringe connector; 16. a flange; 17. a support frame assembly; 18. a valve seat; 19. rotating the rod; 20. a rotating shaft; 21. a valve disk; 22. a support column; 23. a collar; 24. a base plate.

Detailed Description

In order to make the technical solution of the present invention better understood by those skilled in the art, the present invention will be further described in detail with reference to the accompanying drawings.

The utility model provides a fat tissue digestion and extraction adipose-derived stem cell device as shown in figures 1-5, which comprises a separator 1, a digester 2 and a separation purifier 3, wherein the separator 1 is a central structure with an open upper end, a sealing plate 6 is arranged at the top of the separator 1, a first injector connector 4 and a second injector connector 5 which are communicated with the inside of the separator 1 are arranged on the sealing plate 6, a steel bar screen 7 is arranged on the inner wall of the separator 1, and a communicating pipe 8 communicated with the digester 2 is arranged at the bottom of the separator 1; the digester 2 and the separation purifier 3 are both of a central structure and are communicated, a switch valve assembly 12 is installed at the communication position of the digester 2 and the separation purifier 3, a first cell screen 10 is installed inside the digester 2, the first cell screen 10 is located at the bottom of the communication pipe 8, a third injector connector 9 is installed at one end, away from the separation purifier 3, of the digester 2, a waste liquid discharge pipe 11 is installed on the digester 2 at the bottom of the first cell screen 10, and a valve is installed on the waste liquid discharge pipe 11; the second cell screen 13 and the sterile polyethylene filter membrane 14 are arranged inside the separation purifier 3, the fourth injector connector 15 is arranged at the center of one end, far away from the digester 2, of the separation purifier 3, the problems that the existing fat particle purification device is low in efficiency and poor in separation effect are solved, grease in the fat stem cell separation and purification process can be efficiently removed in the fat separation and purification process, and the separation yield and the culture efficiency of the fat stem cells are improved to the maximum degree.

Further, in the above technical solution, the first cell screen 10 is a hollow hemispherical structure, and the aperture of the first cell screen 10 is 400um, and the aperture of the second cell screen 13 is 100um;

in particular, the two-cell screen can be used for better screening small pieces of adipose tissues.

Further, in the above technical solution, the sealing plate 6 is an inverted convex structure, the diameter of the bottom of the sealing plate 6 is the same as the inner diameter of the separator 1, and a sealing rubber ring is sleeved outside the bottom of the sealing plate 6;

specifically, the bottom of the sealing plate 6 is inserted into the bottom of the separator 1, and the sealing rubber ring can seal the joint, thereby preventing leakage.

Further, in the above technical scheme, both ends of the communicating pipe 8 are in a threaded structure, and the communicating pipe 8 is connected with the separator 1 and the digester 2 through internal and external threads; the end, far away from each other, of each of the digester 2 and the separation purifier 3 is provided with a break, the breaks of the digester 2 and the separation purifier 3 are provided with flanges 16, and the breaks of the digester 2 and the separation purifier 3 are connected with a plurality of fixing bolts through the two flanges 16;

particularly, convenient dismantlement can be accomplished between each subassembly of this device to be convenient for change and clearance, open flange 16 and bolt, can unpack digester 2 and separation and purification ware 3 apart, thereby clear up or change the filter screen and the filter membrane of inside, guarantee follow-up extraction effect.

Further, in the above technical solution, the switch valve assembly 12 includes a valve seat 18, the valve seat 18 is connected with one end of the digester 2 and the separation purifier 3 close to each other, a rotating shaft 20 is installed in the valve seat 18, a rotating rod 19 is installed at the bottom of the valve seat 18, the rotating rod 19 is connected with the rotating shaft 20, and a valve disc 21 is installed on the rotating shaft 20;

specifically, the rotating shaft 20 can drive the valve disc 21 to rotate by rotating the rotating rod 19 at the bottom, so that the valve seat 18 is opened, and the digester 2 and the separation purifier 3 are communicated.

Further, in the above technical solution, the outer sides of the separator 1, the digester 2 and the separation purifier 3 are all provided with a support frame assembly 17, the support frame assembly 17 comprises a lantern ring 23, the bottom of the lantern ring 23 is provided with a support column 22, the bottom of the support column 22 is provided with a base plate 24, and the support column 22 of the support frame assembly 17 outside the separator 1 is of an L-shaped structure;

collars 23 are fitted over the outside of the separator 1, digester 2 and separation and purification unit 3, after which a firm support of the unit is achieved by means of support posts 22 and base plates 24.

This practical theory of operation:

when the device is used, the first syringe connector 4 is connected with an external adipose tissue collection syringe, and the second syringe connector 5 is connected with an external normal saline usage syringe; the resulting fat and saline are pumped into separator 1 to disperse the fat and allow it to get a full flush of saline. Fat falls from the upper end of separator 1 to the lower extreme of separator 1, and in this whereabouts in-process, little blood clot, fat piece, fibrous tissue piece are filtered on steel bar screen cloth 7 of corresponding position department, just so can accomplish the preliminary separation of fat cell. The separation liquid enters the digester 2 from a communicating pipe 8 communicated with the digester 2;

in the digester 2, the fat tissue fluid is doped with impurities such as small clotted blood, fat fragments, fibrous tissue blocks, tumescent fluid and the like, and passes through the first cell screen 10, the waste fluid is filtered out and enters the cavity of the digester 2, the waste fluid discharge pipe 11 is opened to discharge the waste fluid, and the waste fluid discharge pipe 11 is closed. The fat tissue cut by the steel bar screen 7 is caught in the steel bar screen 7, and digestive enzymes are added from a third syringe connector 9 communicating with the inside of the digester 2 to perform digestion of the fat tissue, and the liquid can be contacted with the fat tissue through the first cell screen 10 to perform digestion. After digestion is finished, the third syringe connector 9 communicated with the inside is filled with normal saline, and then the switch valve component 12 is opened;

the digestive juice enters the separation purifier 3, the aseptic syringe is connected into a fourth syringe connector 15 communicated with the inside of the separation purifier 3 for drawing, the fat tissue digestive juice passes through a second cell screen 13 and an aseptic polyethylene filter membrane 14 by negative pressure, the single cell is screened, the grease floating on the liquid level is attached to the surface of the aseptic polyethylene filter membrane 14, the liquid is collected for centrifugation, and the cell suspension is subjected to plating culture, so that the separation yield and the culture efficiency of the adipose-derived stem cells are improved to the maximum extent.

While certain exemplary embodiments of the present invention have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that the described embodiments may be modified in various different ways without departing from the spirit and scope of the present invention. Accordingly, the drawings and description are illustrative in nature and should not be construed as limiting the scope of the invention.

Claims

1. An adipose tissue digestion and extraction adipose-derived stem cell device comprises a separator (1), a digester (2) and a separation purifier (3), and is characterized in that: the separator (1) is of a central structure with an opening at the upper end, a sealing plate (6) is installed at the top of the separator (1), a first injector connector (4) and a second injector connector (5) which are communicated with the inside of the separator (1) are arranged on the sealing plate (6), a steel bar screen (7) is installed on the inner wall of the separator (1), and a communicating pipe (8) communicated with the digester (2) is arranged at the bottom of the separator (1);

the digestion device (2) and the separation purifier (3) are both of a central structure and are communicated, a switch valve assembly (12) is installed at the communication position of the digestion device (2) and the separation purifier (3), a first cell screen (10) is installed inside the digestion device (2), the first cell screen (10) is located at the bottom of the communicating pipe (8), a third injector connector (9) is installed at one end, far away from the separation purifier (3), of the digestion device (2), a waste liquid discharge pipe (11) is installed on the digestion device (2) at the bottom of the first cell screen (10), and a valve is installed on the waste liquid discharge pipe (11);

the inside of separation and purification ware (3) is installed second cell screen cloth (13) and aseptic polyethylene filter membrane (14), separation and purification ware (3) are kept away from digester (2) one end center department and are installed fourth syringe connector (15).

2. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: first cell screen cloth (10) are hollow hemispherical structure, just the aperture of first cell screen cloth (10) is 400um, the aperture of second cell screen cloth (13) is 100um.

3. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: the sealing plate (6) is of an inverted convex structure, the diameter of the bottom of the sealing plate (6) is the same as the inner diameter of the separator (1), and a sealing rubber ring is sleeved on the outer side of the bottom of the sealing plate (6).

4. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: both ends of the communicating pipe (8) are of threaded structures, and the communicating pipe (8) is connected with the separator (1) and the digester (2) in an internal and external threaded mode.

5. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: switch valve module (12) are including disk seat (18), disk seat (18) are close to one end each other with digester (2) and separation and purification ware (3) and are connected, install pivot (20) in disk seat (18), dwang (19) are installed to the bottom of disk seat (18), dwang (19) are connected with pivot (20), install valve dish (21) on pivot (20).

6. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: one end of the digester (2) and one end of the separation purifier (3) which are far away from each other are provided with a breaking part, the breaking parts of the digester (2) and the separation purifier (3) are provided with flanges (16), and the breaking parts of the digester (2) and the separation purifier (3) are connected with a plurality of fixing bolts through two flanges (16).

7. The adipose tissue digestion adipose-derived stem cell device of claim 1, wherein: support frame subassembly (17) are all installed in the outside of separator (1) and digester (2) and separation and purification ware (3), support frame subassembly (17) is including lantern ring (23), support column (22) are installed to the bottom of lantern ring (23), base plate (24) are installed to the bottom of support column (22), support column (22) of support frame subassembly (17) in the separator (1) outside are L type structure.