CN108823073A - A kind of recovering device and collecting method of microalgae cell - Google Patents
A kind of recovering device and collecting method of microalgae cell Download PDFInfo
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- CN108823073A CN108823073A CN201811095175.XA CN201811095175A CN108823073A CN 108823073 A CN108823073 A CN 108823073A CN 201811095175 A CN201811095175 A CN 201811095175A CN 108823073 A CN108823073 A CN 108823073A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The present invention relates to a kind of recovering device of microalgae cell and methods, including inlet tube, filter residue bed, harvest bed, spraying-rinsing pipe, culture solution collecting tank, algal gel delivery outlet, waste liquid outlet, fresh water pump, residue collection slot, filter residue cleaning bar, filtered water tank, rotating wheel, sludge outlet, slagging channel, rotation axis etc., the inlet tube is connect with filter residue bed, filter residue bed, algal gel delivery outlet is connect with harvesting bed respectively, harvesting bed is connect with culture solution collecting tank, the setting of spraying-rinsing pipe is inside harvesting bed, residue collection slot is connect with filter residue bed, sludge outlet is arranged in residue collection slot, rotation axis and filtered water tank, rotating wheel connection.The present invention quickly harvests microalgae cell from 0 ° to the harvesting bed that 90 ° of consecutive variations or echelon change by inclination angle, both being completely separated and sufficiently cleaning for microalgae cell and culture solution had been ensure that, microalgae cell is efficiently avoided again, unsmooth problem is discharged, realize the efficient harvesting of microalgae cell.
Description
Technical field
The present invention relates to the harvesting technique field of microalgae more particularly to recovering device and the harvesting sides of a kind of microalgae cell
Method.
Background technique
Microalgae is a kind of widely distributed autophyte in the natural environment, can not only pass through photosynthesis, synthetic oil
The compound of the high added values such as rouge, protein, glycosylglycerol, moreover, because photosynthetic efficiency is high, growth is fast, unit per mu yield
The features such as high, is considered one of most important grain resource of possible human future by international food and agricultural organization.However, due to frustule
Volume is small, causes harvesting difficulty and harvesting is at high cost, the difficult point for becoming restriction microalgae industry development and large-scale promotion is asked
One of topic.Common microalgae recovery method mainly has flocculating setting, filtering or centrifuge separation etc., and flocculating setting needs that flocculation is added
Agent causes breeding wastewater reuse difficult, causes water resource pollution and waste, and industrialization microalgae recovery is difficult to use.Centrifuge separation
It is at high cost, it is desirable that high-speed rotating kinetic energy, harvesting energy consumption is high, and mainly laboratory harvests analysis on a small scale and uses.Therefore, mistake
It has filtered into main microalgae recovery means, domestic and international main filtering collecting method is the sieve utensil by manual control come complete
At, such as spirulina using the soft strainer of 300-380 purpose be made plansifter, pocket sieve or the unpowered sifter device of inclined screen, pass through single-stage
Inclined-plane filter bed or multistage inclined-plane filter bed realize harvesting.This recovering device, algae solution such as cascaded are flowed through from inclined-plane filter bed, moisture
Through strainer, algal gel is attached on wire side, needs washing away using water flow, the device of this structure, not only recovering device takes up an area
Area is very big, and construction cost is also higher, and needs uninterrupted current scour, prevents algal gel accumulation from influencing harvesting, unmanned pipe
It is big to manage difficulty.In addition, the algal gel that this plansifter or inclined screen platform are harvested needs to flow into a harvesting pond first, need to utilize
The extra powers such as piston pump transfer to algal gel dewaterer or other drying equipments or container and carry out deep processing, and process flow is long, adopts
Receive inefficiency.To solve plansifter or tilting the harvest efficiency of screen device lowly and the realistic problem that takes up a large area, China is specially
Benefit application CN102696340A proposes a kind of harvesting of the algae solution that tilting drum filtering-machine is connected in series and dehydration device, core
It is exactly the driving device using roller, drives multiple rollers rotation separation algae solution algal gel, allow the algae solution being pumped into rotary filter
Interior moving in rotation.Compared with existing unpowered plane sifter device, need to drive bulky harvesting film using powerful driving device
Platform rotation, since harvesting film device is big and clumsy, harvesting power energy consumption is significantly increased, flat with currently used power-free multi-stage
Sieve is compared, and technical advantage is not obvious, and can be accumulated instead because of algal gel in drum filtration film surface, be caused to harvest unsmooth ask
Topic.
To sum up, the recovering device of existing microalgae cell and method still have the problems such as harvesting at high cost, low efficiency, because
This, it is necessary to study a kind of recovering device of new microalgae cell.
Summary of the invention
For above-mentioned problems of the prior art, the present invention is intended to provide a kind of recovering device of microalgae cell and side
Method.The present invention quickly harvests microalgae cell from 0 ° to the harvesting bed that 90 ° of consecutive variations or echelon change by inclination angle,
Not only it ensure that being completely separated and sufficiently cleaning for microalgae cell and culture solution, but also to efficiently avoid microalgae cell discharge unsmooth,
It is deposited in the problems in harvesting bed, the harvest efficiency of microalgae cell is greatly improved, realizes the efficient harvesting of microalgae cell.
An object of the present invention is to provide a kind of recovering device of microalgae cell.
The second object of the present invention is to provide a kind of collecting method of microalgae cell.
The third object of the present invention is to provide a kind of method of spirulina for harvesting synthetic glycerine glucoside.
For achieving the above object, specifically, the invention discloses following technical proposals：
Firstly, the present invention provides a kind of recovering device of microalgae cell, including：Filter residue bed, harvesting bed, the filter residue bed are set
It sets above harvesting bed, and the central axis of the two is overlapped；The filter residue bed is the circle that lower end surface diameter is greater than upper surface diameter
Pyramidal structure, side form the bed surface of filter structure, i.e. filter residue bed bed surface, in order to harvesting microalgae cell and culture
Large granular impurity in liquid mixture is filtered.
The harvesting bed is plane of bending loudspeaker funnel type structure, and side wall forms the bed surface of fenestra filter structure, that is, harvests
Bed bed surface, the inclined angle alpha of bed surface with respect to the horizontal plane are changed from 0 ° to 90 ° of consecutive variations or echelon.It is filtered out by filter residue bed miscellaneous
The microalgae cell and culture solution of matter, which enter, harvests bed bed surface, in flow process, fenestra of most of culture solution from harvesting bed bed surface
It filters and microalgae cell is trapped on bed surface, realizes the quick separating of microalgae cell and culture solution, separate most of culture
The microalgae cell of liquid forms algal gel, is discharged from the algal gel delivery outlet of harvesting bed bottom after collecting.
Preferably, the inclined angle alpha echelon variation of the harvesting bed bed surface with respect to the horizontal plane is divided into 4 gradients：5°,15°,
The characteristics of 45 °, 85 °, this change of gradient is that the starting gradient of bed surface is relatively slow, keeps bed surface separating area larger, is conducive to big
The quick separating for measuring culture solution harvests the bed surface gradient and quickly increases, bed surface separating area is quick with the quick separating of culture solution
It shrinks, is conducive to microalgae cell flowing in this way and is quickly discharged harvest bed, prevent microalgae cell from accumulating.
Further, the recovering device further includes inlet tube, and the inlet tube is fixed on the bed surface of filter residue bed in a ring
Upper end, and liquid distributing hole of several jet directions towards filter residue bed is provided on inlet tube, the setting of liquid distributing hole realize containing
Equilibrium assignment of the culture solution of microalgae cell on filter residue bed bed surface.
Preferably, the filter residue bed bed surface is fenestra filter structure, and the fenestra filter structure is by fenestra 10-100 purpose
Hard or soft filter material are made；The filter residue bed of this pore diameter range can not only be such that microalgae cell passes through, but also can incite somebody to action
Contaminant filter realizes efficiently separating for microalgae cell and impurity on filter residue bed bed surface.
Preferably, the harvesting bed bed surface is fenestra filter structure, and the fenestra filter structure is by fenestra 100-800 purpose
Hard or soft filter material are made；The harvesting bed of this pore diameter range can both stay in microalgae cell on harvesting bed bed surface,
It does not influence passing through for liquid in microalgae cell again, can effectively realize the harvesting to microalgae cell.
Further, the recovering device further includes residue collection slot, filter residue cleaning bar, rotating wheel, sludge outlet, row
Slag channel, rotation axis；The periphery of the lower end surface of filter residue bed is arranged in the residue collection slot, and closely connect with filter residue bed, filter
Slag outlet is arranged in residue collection slot, and slagging channel is connect with sludge outlet, and the filter residue cleaning bar and rotation axis connect
It connects, and circular-rotation can be done along residue collection slot under the driving of rotation axis, it is miscellaneous in order to be filtered down to filter residue bed
Matter is uninterruptedly cleared up, and is successively discharged by sludge outlet, slagging channel, prevents impurity from accumulating influence in residue collection slot
Filter effect；And rotation axis, filter residue bed, the central axis coincidence for harvesting bed three.
One end of the rotation axis is located in harvesting bed, and the other end is connect after passing through filter residue bed with rotating wheel, and rotation axis,
Filter residue bed, the central axis for harvesting bed three are overlapped, and the effect of the rotating wheel is that driving force is provided for rotation axis, the filter residue
Collecting tank is for collecting the impurity filtered out from microalgae cell and culture solution.
Preferably, the sludge outlet is the aperture of one or more suitable shapes, specified in order to which impurity to be discharged to
Region.
It is furthermore preferred that the sludge outlet is 2 round holes, it is symmetrically dispersed on the bottom surface of residue collection slot, it can
Effectively improve the velocity of discharge and efficiency of filter residue.
Further, the recovering device further includes cleaning sparge pipe, and the cleaning sparge pipe is located in harvesting bed, cleaning
Hole for water spraying is provided on sparge pipe, the jet direction of hole for water spraying is flowed towards harvesting bed, and with harvesting bed bed surface or microalgae cell
The inclined angle in direction.Cleaning sparge pipe is mainly used for cleaning and washing away the nutrition on the microalgae cell surface on harvesting bed bed surface
Microalgae cell is washed into algal gel delivery outlet discharge harvesting bed while cleaning microalgae cell by the foreign matters such as salt, flora.
Preferably, the inclination angle between the jet direction of the hole for water spraying and harvesting bed bed surface or microalgae cell flow direction
It is 45 ° -90 °.
Further, the recovering device further includes filtered water tank, and filtered water tank is arranged outside recovering device, and filtered water tank with
One end connection for cleaning sparge pipe, the other end for cleaning sparge pipe are located in harvesting bed, clear water in the filtered water tank be without
Salt or saliferous clean water with culture solution isoconcentration need to use and culture when harvesting the frustule containing glycosylglycerol
The saliferous clean water of liquid isoconcentration prevents the glycosylglycerol in frustule from secreting into cleaning solution, causes as cleaning solution
The loss of glycosylglycerol.
Further, the rotation axis is hollow structure, and one end of rotation axis is located inside harvesting bed, and the other end passes through filter
Slag-bed is connected to filtered water tank again after connecting with rotating wheel, and the one or both ends of the cleaning sparge pipe are located at harvesting with rotation axis
Part connection in bed, and cleaning sparge pipe can be rotated together with rotation axis, and eject ejected wash water to harvesting bed surface.
Further, the recovering device further includes support frame, fresh water pump, clear water input port, dynamic sealing component, described clear
Water tank fixation is provided with clear water input port on the support frame, on filtered water tank, passes through dynamic sealing component between rotation axis and filtered water tank
Connection, the clear water input port is connect with fresh water pump.
Preferably, the dynamic sealing component includes the movable sealing structure of packing seal, mechanical seal.
Further, the recovering device further includes culture solution collecting tank, and the culture solution collecting tank setting is in harvesting bed
Bed surface lower part harvests the algal gel delivery outlet of lower end formation of bed through training for collecting the liquid filtered down from harvesting bed
The bottom surface of nutrient solution collecting tank, and algal gel delivery outlet is collected the position that groove bottom contacts with culture solution and is tightly connected, and culture solution is prevented
It is mixed again with microalgae cell after culture solution leakage in collecting tank.
Enter by the deimpurity microalgae cell of filter residue bed filter and culture solution and harvests bed bed surface, in flow process, culture solution
With microalgae cell quick separating, microalgae cell is discharged from the algal gel delivery outlet of harvesting bed bottom after collecting, and culture solution then leads to
The fenestra filter structure crossed on harvesting bed bed surface enters culture solution collecting tank.
Further, the lower part of the culture solution collecting tank is provided with waste liquid outlet, and being convenient for will be in culture solution collecting tank
Culture solution collect after unified discharge.
Further, the recovering device further includes algae solution delivery pump, and the inlet tube connect with algae solution delivery pump, is used for
Microalgae cell and culture solution are transported in inlet tube.
Secondly, the present invention provides a kind of collecting method of microalgae cell, include the following steps：
(1) microalgae cell and culture solution are distributed to the bed surface of filter residue bed, pass through the filter structure pair of the bed surface of filter residue bed
Impurity in microalgae cell and culture solution is filtered；
(2) deimpurity microalgae cell is filtered by step (1) and culture solution enters harvesting bed bed surface, make culture solution and microalgae
Cell quick separating, microalgae cell are discharged from the algal gel delivery outlet of harvesting bed bottom after collecting.
Finally, the present invention provides a kind of method of spirulina for harvesting synthetic glycerine glucoside, include the following steps：
(1) firstly, spirulina and culture solution are sent into inlet tube by algae solution delivery pump, inlet tube is by spirulina and culture
Liquid is evenly distributed on filter residue bed bed surface by liquid distributing hole, by the fenestra filter structure on bed surface in spirulina and culture solution
Impurity be filtered, after impurity collects in residue collection slot, removed from sludge outlet by filter residue cleaning bar into filter
Slag channel finally cleans out recovery system, and filters out the spirulina of impurity and culture solution enters harvesting bed；
(2) deimpurity spirulina being filtered by step (1) and culture solution entering harvesting bed bed surface, bed surface is with respect to the horizontal plane
Inclined angle alpha changed from 0 ° to 90 ° of consecutive variations or echelon；Meanwhile the saliferous with culture solution isoconcentration being arranged in filtered water tank
Clean water is by entering cleaning sparge pipe after hollow rotation axis, with the rotation of rotation axis, spray of the clear water from spraying-rinsing pipe
The spirulina on harvesting bed bed surface is uniformly cleaned after spraying in water hole, and the jet direction of hole for water spraying and spirulina flow
Direction be in 45 ° of -90 ° of tilt angles, after collecting culture solution and spirulina quick separating, spirulina from harvesting bed bottom algae
It is discharged in mud delivery outlet, the separating and extracting for next step；And culture solution then enters training by the fenestra filter structure on bed surface
It is discharged after nutrient solution collecting tank from waste liquid outlet.
Compared with prior art, the beneficial effect that the present invention obtains is：Compared with traditional harvesting bed, the present invention is using filter
Laminated construction above and below slag-bed and harvesting bed, saves the occupied area of equipment arrangement, the large granular impurity that filter residue bed filters out is logical
It crosses cleaning bar to clear up away in time, improves the ability of device processing waste.In addition, harvesting bed bed surface structure is used inclination
Angle be by 0-90 ° of continuous or echelon variation plane of bending loudspeaker funnel-shaped structure, its main feature is that：Microalgae cell and culture solution just into
When entering bed surface, the gradient of bed surface is relatively slow, and bed surface separating area is larger, is conducive to the quick separating of mass propgation liquid, with culture
The quick separating of liquid harvests bed surface rapid desufflation, and the inclination angle of bed surface also constantly increases, and is conducive to microalgae cell flowing and quickly
Discharge harvesting bed, prevents microalgae cell from accumulating problem, to increase substantially harvest efficiency.
Detailed description of the invention
The accompanying drawings constituting a part of this application is used to provide further understanding of the present application, and the application's shows
Meaning property embodiment and its explanation are not constituted an undue limitation on the present application for explaining the application.
Fig. 1 is the recovering device structural schematic diagram of microalgae cell of the present invention.
Fig. 2 is the structural schematic diagram of filter residue bed and harvesting bed in embodiment 1.
Fig. 3 is the structural schematic diagram of filter residue bed and harvesting bed in embodiment 2.
Fig. 4 is filtered water tank of the present invention and rotation axis packing seal connection schematic diagram.
Attached drawing acceptance of the bid note respectively represents：1- inlet tube, 2- filter residue bed, 3- harvesting bed, 4- spraying-rinsing pipe, 5- culture solution are received
Collect slot, 6- algal gel delivery outlet, 7- waste liquid outlet, 8- fresh water pump, 9- residue collection slot, 10- filter residue cleaning bar, 11- filtered water tank,
12- rotating wheel, 13- sludge outlet, 14- algae solution delivery pump, 15- rotation axis, 16- support frame, 17- dynamic sealing component, 18- are clear
Water input, 19- slagging channel.
Specific embodiment
It is noted that following detailed description is all illustrative, it is intended to be further explained to the application offer.Unless another
It indicates, all technical and scientific terms used herein has usual with the application person of an ordinary skill in the technical field
The identical meanings of understanding.
It should be noted that term used herein above is merely to describe specific embodiment, and be not intended to restricted root
According to the illustrative embodiments of the application.As used herein, unless the context clearly indicates otherwise, otherwise singular
Also it is intended to include plural form, additionally, it should be understood that, when in the present specification using term "comprising" and/or " packet
Include " when, indicate existing characteristics, step, operation, device, component and/or their combination.
As background technique is introduced, the recovering device and method of existing microalgae cell still have harvesting cost
The problems such as height, low efficiency, therefore, the invention proposes a kind of recovering device of microalgae cell and collecting methods, below with reference to attached
Figure and specific embodiment are described further the present invention.
Embodiment 1
As shown in Fig. 1,2,4, a kind of recovering device of microalgae cell, including：Inlet tube 1, filter residue bed 2, harvesting bed 3, spray
Water cleaning pipe 4, culture solution collecting tank 5, algal gel delivery outlet 6, waste liquid outlet 7, fresh water pump 8, residue collection slot 9, filter residue clean
Bar 10, filtered water tank 11, rotating wheel 12, sludge outlet 13, algae solution delivery pump 14, rotation axis 15, support frame 16, dynamic sealing component
17, clear water input port 18, slagging channel 19.
The inlet tube 1 is connect with algae solution delivery pump 14, and the filter residue bed 2 is that lower end surface diameter is greater than upper surface diameter
Circular cone shape structure, and the side of the frustum of a cone forms the bed surface (filter bed bed surface) of filter structure, inlet tube 1 is fixed in a ring
The bed surface upper end of filter residue bed, and liquid distributing hole of the jet direction towards filter residue bed 2 is provided on inlet tube 1.2 bed surface of filter residue bed
For fenestra filter structure, it is made of the soft filter cloth of 50 mesh of fenestra.
The top of harvesting bed 3 is arranged in the filter residue bed 2, and the central axis of the two is overlapped, and the lower end of harvesting bed 3 is formed
Algal gel delivery outlet 6；The lower end surface periphery of the filter residue bed 2 is provided with residue collection slot 9, is arranged on the bottom surface of residue collection slot 9
There is sludge outlet 13, slagging channel 19 is connect with sludge outlet 13.
It is provided in the residue collection slot 9 and is connect with rotation axis 15, and can be under the driving of rotation axis 15 along filter
Slag collecting tank 9 does the filter residue cleaning bar 10 of circular-rotation.
The harvesting bed 3 is funnel type structure, and side wall forms the bed surface (harvesting bed bed surface) of filter structure, and bed surface is opposite
In horizontal plane inclined angle alpha by 0-90 ° of consecutive variations, filter deimpurity microalgae cell by filter residue bed 2 and culture solution enter and adopts
The bed surface for receiving bed 3, in flow process, culture solution and microalgae cell quick separating, microalgae cell collect the algae from harvesting 3 bottom of bed
It is discharged in mud delivery outlet 6, and culture solution then enters culture solution collecting tank 5, the harvesting by the fenestra filter structure on bed surface
The bed surface of bed 3 is fenestra filter structure, is made of the hard filter cloth of 300 mesh of fenestra.
The setting of spraying-rinsing pipe 4 is in harvesting bed 3, and the rotation axis 15 is hollow structure, and in rotation axis 15
Mandrel line is overlapped with the central axis of harvesting bed 3, and one end of rotation axis 15 is located in harvesting bed 3, spraying-rinsing pipe 4 and rotation axis
15 protrude into the part connection in harvesting bed 3, and several hole for water sprayings are provided on spraying-rinsing pipe 4, the jet direction face of hole for water spraying
It is in 45 ° of tilt angles to harvesting bed 3, and with the direction of microalgae cell flowing；The other end of rotation axis 15 is connected to filtered water tank, institute
The top that rotation axis 15 is arranged in filtered water tank 11 is stated, and is fixed on support frame 16, clear water input port is provided on filtered water tank 11
18, it is connected between rotation axis 15 and filtered water tank 11 by dynamic sealing component, the clear water input port 18 is connect with fresh water pump 8, institute
State the movable sealing structure that dynamic sealing component 17 is packing seal.
One end of the rotation axis 15 is located in harvesting bed, and the other end is connect after passing through filter residue bed 2 with rotating wheel 12, and turns
Moving axis 15, filter residue bed 2, the central axis coincidence for harvesting 3 three of bed, the rotating wheel 12 provide driving for the rotation of rotation axis 15
Power, clear water in filtered water tank 11 by entering spraying-rinsing pipe after hollow rotation axis 15, one end of the cleaning sparge pipe with
Rotation axis connection or both ends are connected to rotation axis, and with the rotation of rotation axis 15, clear water is sprayed from the hole for water spraying of spraying-rinsing pipe 4
The microalgae cell on harvesting bed bed surface is uniformly cleaned after out, and while cleaning microalgae cell, by microalgae cell
It is washed into the discharge harvesting bed 3 of algal gel delivery outlet 6.
The bed surface lower part of harvesting bed 3, the algal gel output that the lower end of harvesting bed 3 is formed is arranged in the culture solution collecting tank 5
Mouth 6 runs through the bottom surface of culture solution collecting tank 5, and the position sealing that algal gel delivery outlet 6 is contacted with 5 bottom surface of culture solution collecting tank connects
It connects.
The lower part of the culture solution collecting tank 5 is provided with waste liquid outlet 7, and culture solution is discharged by waste liquid outlet 7.
Embodiment 2
As shown in figure 3, a kind of recovering device of microalgae cell, with embodiment 1, difference is：(1) the filter residue bed 2
Bed surface is made of fenestra of the hard membrane material of 80 mesh.
(2) bed surface of the harvesting bed 3 is made of fenestra of the soft filter cloth of 100 mesh.
(3) the inclined angle alpha echelon variation of the bed surface of the harvesting bed 3 with respect to the horizontal plane is divided into 4 gradients, is followed successively by α1
=5 °, α2=15 °, α3=45 °, α4=85 °.
Embodiment 3
A kind of recovering device of microalgae cell, with embodiment 1, difference is：(1) bed surface of the filter residue bed 2 is by fenestra
It is made of the hard membrane material of 30 mesh.
(2) bed surface of the harvesting bed 3 is made of fenestra of the soft filter cloth of 400 mesh.
(3) direction of the jet direction of hole for water spraying and algal gel flowing is in 90 ° of tilt angles on the spraying-rinsing pipe 4.
(4) the dynamic sealing component 17 is the movable sealing structure of mechanical seal.
Embodiment 4
A kind of recovering device of microalgae cell, with embodiment 1, difference is：(1) bed surface of the filter residue bed 2 is by fenestra
It is made of the soft filter cloth material of 100 mesh.
(2) bed surface of the harvesting bed 3 is made of fenestra of the soft filter cloth of 600 mesh.
(3) direction of the jet direction of hole for water spraying and algal gel flowing is in 60 ° of tilt angles on the spraying-rinsing pipe 4.
(4) the dynamic sealing component 17 is the movable sealing structure of mechanical seal.
Embodiment 5
A kind of recovering device of microalgae cell, with embodiment 1, difference is：(1) bed surface of the filter residue bed 2 is by fenestra
It is made of the hard membrane material of 10 mesh.
(2) bed surface of the harvesting bed 3 is made of fenestra of the soft film of 800 purposes.
(3) direction of the jet direction of hole for water spraying and algal gel flowing is in 75 ° of tilt angles on the spraying-rinsing pipe 4.
(4) the dynamic sealing component 17 is the movable sealing structure of mechanical seal.
Embodiment 6
A method of the spirulina of harvesting synthetic glycerine glucoside includes the following steps：
(1) firstly, the intracellular microalgae rich in glycosylglycerol, culture solution are sent into feed liquor by algae solution delivery pump 14
Spirulina and culture solution are evenly distributed to the bed surface of filter residue bed 2 by liquid distributing hole, pass through the bed of filter residue bed 2 by pipe 1, inlet tube 1
Fenestra filter structure on face is filtered the impurity in spirulina and culture solution, after impurity collects in residue collection slot 9,
It is removed from sludge outlet 13 by filter residue cleaning bar 10 and enters filter residue channel 19, finally clean out recovery system, and filtered out
The spirulina and culture solution of impurity enter harvesting bed 3；
(2) deimpurity spirulina is filtered by step (1) and culture solution enters the bed surface of harvesting bed 3, meanwhile, in filtered water tank
Clear water by entering spraying-rinsing pipe 4 after hollow rotation axis 15, with the rotation of rotation axis 15, clear water is from spraying-rinsing pipe
After 4 hole for water spraying sprays, the spirulina on harvesting bed bed surface is uniformly cleaned, and the jet direction and spiral of hole for water spraying
The direction of algae flowing is in 45 ° of -90 ° of tilt angles, from harvesting bed 3 after collecting culture solution and spirulina quick separating, spirulina
It is discharged in the algal gel delivery outlet 6 of bottom, the separating and extracting for next step glycosylglycerol；And culture solution then passes through on bed surface
Fenestra filter structure enter after culture solution collecting tank 5 and enter culture solution recyclable device 12 from waste liquid outlet 7, to culture solution
It is recycled, then by being re-used for the culture of spirulina after liquid valve door 13.
(2) deimpurity spirulina being filtered by step (1) and culture solution entering harvesting bed bed surface, bed surface is with respect to the horizontal plane
Inclined angle alpha changed from 0 ° to 90 ° of consecutive variations or echelon；Meanwhile the saliferous with culture solution isoconcentration being arranged in filtered water tank
Clean water cleans sparge pipe by entering after hollow rotation axis,
The foregoing is merely preferred embodiment of the present application, are not intended to limit this application, for those skilled in the art
For member, various changes and changes are possible in this application.Within the spirit and principles of this application, it is made it is any modification,
Equivalent replacement, improvement etc., should be included within the scope of protection of this application.
Claims (10)
1. a kind of recovering device of microalgae cell, it is characterised in that：The recovery system is made of filter residue bed, harvesting bed；It is described
The setting of filter residue bed is above harvesting bed, and the central axis of the two is overlapped；The lower end for harvesting bed forms algal gel delivery outlet；
The filter residue bed is the circular cone shape structure that lower end surface diameter is greater than upper surface diameter, and the side of the frustum of a cone forms filtering knot
The filter residue bed bed surface of structure；
The harvesting bed is plane of bending loudspeaker funnel type structure, and side wall forms the harvesting bed bed surface of filter structure, harvests bed bed
The inclined angle alpha of face with respect to the horizontal plane is changed from 0 ° to 90 ° of consecutive variations or echelon.
2. the recovering device of microalgae cell as described in claim 1, it is characterised in that：The recovering device further includes feed liquor
Pipe, inlet tube is fixed on the upper end of filter residue bed bed surface in a ring, and several jet directions are provided on inlet tube towards filter residue bed
Liquid distributing hole；
Or, the bed surface of the filter residue bed is fenestra filter structure, preferably by the hard or soft membrane material of fenestra 10-100 mesh
It is made；
Or, the harvesting bed bed surface is fenestra filter structure, the preferably hard by fenestra 100-800 mesh or soft membrane material system
At；
Or, the inclined angle alpha echelon variation of the harvesting bed bed surface with respect to the horizontal plane is divided into 4 gradients：5°,15°,45°,85°.
3. the recovering device of microalgae cell as claimed in claim 1 or 2, it is characterised in that：The recovery system further includes filter
Slag collecting tank, filter residue cleaning bar, rotating wheel, sludge outlet, slagging channel, rotation axis；The residue collection slot setting is being filtered
The periphery of the lower end surface of slag-bed, and closely connect with filter residue bed, the sludge outlet is arranged in residue collection slot, and deslagging is logical
Road is connect with sludge outlet, the filter residue cleaning bar and rotation axis connection, and can be under the driving of rotation axis along filter residue
Collecting tank does circular-rotation, and one end of the rotation axis is located in harvesting bed, and the other end is connect after passing through filter residue bed with rotating wheel,
And rotation axis, filter residue bed, the central axis coincidence for harvesting bed three.
4. the recovering device of microalgae cell as claimed in claim 3, it is characterised in that：The recovering device further includes cleaning spray
Water pipe, the cleaning sparge pipe are located in harvesting bed, clean on sparge pipe and are provided with hole for water spraying, the jet direction of hole for water spraying towards
Harvest bed, and the jet direction of hole for water spraying and harvesting bed bed surface or the inclined angle in direction of microalgae cell flowing；
Preferably, the recovering device further includes filtered water tank, and filtered water tank is arranged outside recovering device, and filtered water tank and cleaning are sprayed
One end of water pipe connects, and the other end for cleaning sparge pipe is located in harvesting bed.
5. the recovering device of microalgae cell as claimed in claim 4, it is characterised in that：The rotation axis is hollow structure, is turned
One end of moving axis is located inside harvesting bed, and the other end is passed through after filter residue bed is connect with rotating wheel and is connected to again with filtered water tank, described clear
The one or both ends for washing sparge pipe are connected to part of the rotation axis in harvesting bed, and cleaning sparge pipe can be with rotation axis
It rotates together, and ejects ejected wash water to harvesting bed surface.
6. the recovering device of microalgae cell as claimed in claim 5, it is characterised in that：The recovering device further includes support
Frame, fresh water pump, clear water input port, the filtered water tank is fixed on the support frame, and the clear water input port is arranged on fresh water pump, clearly
Water pump is connect with clear water input port.
7. the recovering device of microalgae cell as claimed in claim 6, it is characterised in that：The jet direction of the hole for water spraying with it is micro-
Inclination angle between frustule flow direction is 45 ° -90 °；
Or, the dynamic sealing component includes the movable sealing structure of packing seal, mechanical seal.
8. the recovering device of microalgae cell as claimed in claim 4, it is characterised in that：The recovering device further includes culture solution
Collecting tank, the culture solution collecting tank setting run through in harvesting bed bed surface lower part, the algal gel delivery outlet for harvesting the lower end formation of bed
The bottom surface of culture solution collecting tank, and algal gel delivery outlet is collected the position that groove bottom contacts with culture solution and is tightly connected, and prevents from cultivating
It is mixed again with microalgae cell after culture solution leakage in liquid collecting tank；
Preferably, the lower part of the culture solution collecting tank is provided with waste liquid outlet.
9. the method for carrying out microalgae cell harvesting using the described in any item recovering devices of such as claim 1-8, it is characterised in that：
Include the following steps：
(1) microalgae cell and culture solution are distributed to the bed surface of filter residue bed, by the filter structure of the bed surface of filter residue bed to microalgae
Impurity in cell and culture solution is filtered；
(2) deimpurity microalgae cell is filtered by step (1) and culture solution enters harvesting bed bed surface, make culture solution and microalgae cell
Quick separating, microalgae cell are discharged from the algal gel delivery outlet of harvesting bed bottom after collecting.
10. a kind of method for the spirulina for capableing of synthetic glycerine glucoside using device as claimed in claim 8 harvesting,
It is characterized in that：Include the following steps：
(1) firstly, spirulina and culture solution are sent into inlet tube by algae solution delivery pump, inlet tube leads to spirulina and culture solution
It crosses liquid distributing hole to be evenly distributed on filter residue bed bed surface, by the fenestra filter structure on bed surface to miscellaneous in spirulina and culture solution
Matter is filtered, and after impurity collects in residue collection slot, is removed from sludge outlet into filter residue and is led to by filter residue cleaning bar
Road finally cleans out recovery system, and filters out the spirulina of impurity and culture solution enters harvesting bed；
(2) it by step (1) filters deimpurity spirulina and culture solution enters harvesting bed bed surface, bed surface inclining with respect to the horizontal plane
Bevel angle α is changed from 0 ° to 90 ° of consecutive variations or echelon；Meanwhile it is being arranged in filtered water tank clean with culture solution isoconcentration saliferous
Water is by entering cleaning sparge pipe after hollow rotation axis, with the rotation of rotation axis, hole for water spraying of the clear water from spraying-rinsing pipe
The spirulina on harvesting bed bed surface is uniformly cleaned after ejection, and the side of the jet direction of hole for water spraying and spirulina flowing
To being in 45 ° of -90 ° of tilt angles, the algal gel after collecting culture solution and spirulina quick separating, spirulina from harvesting bed bottom is defeated
It is discharged in outlet, the separating and extracting for next step；And culture solution then enters culture solution by the fenestra filter structure on bed surface
It is discharged after collecting tank from waste liquid outlet.
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Cited By (1)
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| CN110734849A (en) * | 2019-12-06 | 2020-01-31 | 中国科学院青岛生物能源与过程研究所 | Microalgae harvesting and spray liquid leaching system |
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| CN108823073B (en) | 2023-07-04 |
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