CN108823072A - A kind of culture of microalgae cell-harvesting integral system and method - Google Patents

Abstract

The invention relates to an integrated system for cultivating and harvesting microalgae cells, belonging to the technical field of cultivating and harvesting microalgae. The culture system includes: a photoreactor, an air inlet pipe, an agitator, an air outlet, and a light source; one end of the air inlet pipe is inserted into the culture solution of the photoreactor; the agitator is arranged in the photoreactor; the air outlet is arranged On the top of the liquid surface of the culture solution; the light source is arranged inside and/or outside the photoreactor; the recovery system is composed of a filter residue bed and a recovery bed, and the filter residue bed is arranged above the recovery bed; the photoreactor and filter residue in the cultivation system The beds are connected by pipes. After the microalgae cells are cultured by the photosynthetic cultivation process, the microalgae cells are rapidly harvested through the harvesting bed whose inclination angle changes continuously or gradiently from 0° to 90°, which not only ensures the balance between the microalgae cells and the culture solution Thorough separation, and effectively avoid the problem of poor discharge of microalgae cells, greatly improving the harvesting efficiency of microalgae cells.

Description

An integrated system and method for cultivating and harvesting microalgae cells

technical field

The invention relates to the technical field of cultivating and harvesting microalgae, in particular to an integrated system and method for cultivating and harvesting microalgae cells.

Background technique

Microalgae is an autotrophic plant widely distributed in the natural environment. It can not only synthesize high value-added compounds such as oil, sugar, protein, and carotene through photosynthesis, but also because of its high photosynthetic efficiency, fast growth, and unit The yield per mu is high, and it is considered by the International Food and Agriculture Organization to be one of the most important food resources for human beings in the future. Due to the small size of algae cells, it is difficult to harvest and the cost of harvesting is high, which has become one of the difficult problems restricting the development and large-scale promotion of the microalgae industry. For this reason, people have developed the culture device of multiple algae, disclose a kind of algae culture device as patent CN206872835U, comprise: culture vessel is placed in the magnetic induction area of magnetic stirrer, and stirring bar is placed in culture vessel; Heating bar and The clamping end of the temperature sensor is clamped on the bracket, and the heating end of the heating rod and the sensing end of the temperature sensor are all extended into the culture container; the bracket is fixed on the base of the magnetic stirrer; each LED and each flexible circuit board After assembly, it is fixed on the outer wall and bottom of the culture container, and each LED is located between each flexible circuit board and the culture container; compared with the prior art, the temperature control of the algae culture solution is accurate and efficient, and the illumination is uniform and sufficient, but this The structure of the culture device is complex and the manufacturing cost is relatively high.

Harvesting microalgae is also an important part of obtaining metabolites. The commonly used microalgae harvesting methods mainly include flocculation and sedimentation, filtration or centrifugal separation. Flocculants need to be added for flocculation and sedimentation, which makes it difficult to reuse aquaculture wastewater, causing water pollution and Wasteful, industrial microalgae harvesting is difficult to adopt. The cost of centrifugation is high, the kinetic energy of high-speed rotation is required, and the energy consumption of harvesting is extremely high. It is mainly used for small-scale harvesting and analysis in laboratories. Therefore, filtration has become the main microalgae harvesting method. The main filtration and harvesting methods at home and abroad are completed by artificially controlled sieve devices, such as spirulina. Sieves or tilting sieves and other unpowered sieves are harvested through single-stage inclined filter beds or multi-stage inclined filter beds. In this harvesting system, the algae liquid flows through the inclined filter bed like a waterfall, and the water penetrates the filter screen and the algae mud adheres to the screen surface, which needs to be washed by the water flow. The device with this structure not only occupies an area of the harvesting system It is very large, and the construction cost is relatively high, and uninterrupted water flow is required to prevent the accumulation of algae mud, which not only affects the harvest, but also makes unmanned management very difficult. In addition, the algae mud harvested by this flat screen or inclined screen platform needs to flow into a recovery pond first, and it needs to use additional power such as piston pumps to transfer to algae mud dehydrator or other drying equipment or containers for further processing, and the process is long. , low recovery efficiency.

In order to solve the practical problems of low recovery efficiency and large footprint of flat or inclined sieve devices, Chinese patent application CN 102696340 A proposes a device for collecting and dehydrating algae liquid in which inclined drum filters are connected in series. The core is to use the driving device of the drum to drive the multi-stage drum to rotate and separate the algae liquid and algae mud, so that the pumped algae liquid rotates and moves in the drum filter. Compared with the existing unpowered flat sieve, it is necessary to use a high-power drive device to drive the heavy harvesting membrane platform to rotate. Because the harvesting membrane device is large and clumsy, the energy consumption of harvesting power is greatly increased, which is different from the currently commonly used unpowered screen. Compared with the multi-stage flat sieve, the technical advantages are not obvious. On the contrary, the algae mud will accumulate on the surface of the drum filter membrane, resulting in poor harvesting.

In summary, the existing methods and systems for culturing and harvesting microalgae cells still have a series of problems such as complex cultivation process, high cost, large area of harvesting equipment, and low harvesting efficiency. Therefore, it is necessary to study a A new microalgae cell culture and harvesting system.

Contents of the invention

In view of the above-mentioned problems in the prior art, the present invention aims to provide an integrated system and method for cultivating and harvesting microalgae cells. The present invention cultivates the microalgae cells through an integrated photosynthetic breeding process, and then quickly harvests the microalgae cells through the harvesting bed whose inclination angle changes continuously or stepwise from 0° to 90°, which not only ensures the microalgae The thorough separation and cleaning of the cells and the culture medium effectively avoids the problem of poor discharge of the microalgae cells and accumulation in the harvesting bed, greatly improves the harvesting efficiency of the microalgae cells, and realizes the recovery of the microalgae cells. Efficient cultivation and harvesting.

One of the objectives of the present invention is to provide an integrated system for cultivating and harvesting microalgae cells.

The second object of the present invention is to provide a method for cultivating and harvesting microalgae cells.

The third object of the present invention is to provide a method for cultivating-harvest the spirulina that synthesizes glycerol glucoside.

In order to achieve the above-mentioned purpose of the invention, specifically, the present invention discloses the following technical solutions:

First, the present invention provides a microalgae cell culture-harvesting integrated system, including a culture system and a harvesting system; the culture system is used for the cultivation of microalgae cells, and the harvesting system is used for algal cells and culture Rapid separation of liquids.

Specifically, the cultivation system includes: a photoreactor, an air inlet pipe, an agitator, an air outlet, and a light source.

The photoreactor is used to carry microalgae cells and culture fluid, one end of the air inlet pipe is inserted into the culture fluid, and the other end is connected to a gas source containing carbon source gas, thereby providing carbon elements for the photosynthetic growth of microalgae cells; preferably, the The carbon-containing source gas is a gas containing CO ₂ .

The agitator is arranged in the photoreactor, so that the microalgal cells in the photoreactor are more uniform in receiving light, supplying nutrients, and absorbing carbon source gas.

The exhaust port is arranged on the upper part of the liquid surface of the culture solution to keep the gas in and out of the photoreactor smooth, and to ensure that the oxygen produced by the photosynthesis of the microalgae cells can be discharged in time. If it is a photoreactor with an upper opening, it can also No exhaust port is provided.

The harvesting system includes: a filter residue bed and a recovery bed. The filter residue bed is arranged above the recovery bed, and the central axes of the two coincide. After the microalgae cells are cultivated in the photoreactor of the cultivation system, they enter the filter residue bed.

The filter residue bed is a truncated conical structure with a diameter of the lower end surface greater than that of the upper end surface, and its side surface forms a bed surface of a filtering structure, so as to filter large particles of impurities in the mixture of harvested microalgae cells and culture solution.

The recovery bed is a trumpet funnel-shaped structure with variable curved surface, and its side wall forms the bed surface of the membrane pore filter structure, that is, the recovery bed bed surface. The inclination angle α of the bed surface relative to the horizontal plane changes continuously or in steps from 0° to 90° Variety. The microalgae cells and culture fluid that have been filtered out of impurities by the filter residue bed enter the recovery bed surface. During the flow process, most of the culture fluid is filtered out from the membrane pores on the recovery bed surface and the microalgae cells are trapped on the bed surface. Realize the rapid separation of microalgae cells and culture medium, and separate most of the microalgae cells from the culture medium to form algae mud, which is collected and discharged from the algae mud output port at the bottom of the recovery bed.

Preferably, the gradient change of the inclination angle α of the bed surface of the recovery bed relative to the horizontal plane is divided into four gradients: 5°, 15°, 45°, and 85°. The characteristic of this gradient change is that the initial gradient of the bed surface is relatively Slowly, the separation area of the bed surface is larger, which is conducive to the rapid separation of a large amount of culture fluid. With the rapid separation of the culture fluid, the slope of the harvest bed surface increases rapidly, and the separation area of the bed surface shrinks rapidly, which is beneficial to the microalgae cells. Flow and rapid drainage of the recovery bed prevents build-up of microalgae cells.

The light source is arranged inside the photoreactor (i.e. internally illuminated) and/or outside; as long as sufficient light can be provided for the photosynthesis of algae cells, when the light source is arranged outside the photoreactor, direct irradiation can be used The light is provided to the algae cells by means of the culture medium, and the light can also be provided to the algae cells by indirectly irradiating the culture medium through a transparent material.

The form of the photoreactor is not limited, it can be a closed tube reactor, a plate reactor, a cylindrical photoreactor; it can also be an open runway pool; as long as it can be guaranteed to receive light irradiation from the liquid surface, the Microalgae can grow rapidly.

The type of microalgae cells is not limited, it can be green algae, diatoms, cyanobacteria, etc., it can also be cyanobacteria, or other genetically modified photosynthetic microorganisms, etc., as long as it can meet the needs of metabolites. , preferably spirulina.

Preferably, the type of the culture solution is not limited, it can be a seawater culture solution, such as the commonly used f/2 culture solution, or a freshwater culture solution, such as the commonly used BG; it can be an improved acidic culture solution, or It can be an alkaline culture solution, such as Zarrok alkaline culture solution, MC green algae culture solution, etc., as long as it can meet the nutrient supply and other physical and chemical conditions required for the growth of microalgae cells.

Preferably, the culture solution includes nutrients such as water, nitrogen, phosphorus, calcium, magnesium, iron and trace metal nutrient salts.

The material and shape of the intake pipe are not limited, it can be inorganic minerals, such as cement, ceramics, quartz sand, etc.; it can also be plastic, such as polyethylene, polypropylene, rubber; it can also be metal, such as Copper, stainless steel, etc., the shape is not limited, it can be tube, square, round, etc.; the shape and number of vent holes are not limited, single hole, multi-hole; as long as it can ensure that carbon dioxide gas can pass into the culture solution.

The material and shape of the agitator are not limited, and it can be a spiral shape, or a shape similar to a paddle, as long as it can achieve uniform mixing of gas, solid, and liquid.

Preferably, the integrated design of the inlet pipe and the agitator: the agitator is provided with an inlet pipe and an air outlet, and after the carbon-containing source gas passes through the inlet pipe, it enters the culture medium from the air outlet, so that the setting can realize Stir while aerating, so that the incoming gas can be more evenly mixed into the culture solution.

The form of the light source is not limited, it can be natural sunlight or artificial light source, as long as it can provide light wavelengths that can meet the photosynthesis requirements of microalgae cells.

Preferably, the artificial light source includes LED, fluorescent lamp, mercury lamp and the like.

Further, the harvesting system also includes a liquid inlet pipe, the liquid inlet pipe is connected to the cultivation system, and the liquid inlet pipe is fixed on the upper end of the bed surface of the filter residue bed in a ring shape, and the liquid inlet pipe is provided with several jet direction Facing the liquid distribution hole of the filter residue bed, the setting of the liquid distribution hole realizes the balanced distribution of the culture solution containing microalgae cells on the filter residue bed.

Preferably, the bed surface of the filter residue bed is a membrane filter structure, and the membrane filter structure is made of hard or soft filter materials with membrane pores of 10-100 mesh.

Preferably, the surface of the recovery bed is a membrane filter structure, and the membrane filter structure is made of hard or soft filter materials with membrane pores of 100-800 mesh.

Further, the harvesting system also includes a filter residue collection tank, a filter residue cleaning rod, a rotating wheel, a filter residue outlet, a slag discharge channel, and a rotating shaft; the filter residue collection tank is arranged on the outer periphery of the lower end surface of the filter residue bed, and is connected The bed is closely connected, the filter residue discharge port is set in the filter residue collection tank, the residue discharge channel is connected to the filter residue discharge port, the filter residue cleaning rod is connected to the rotating shaft, and can be driven by the rotating shaft to make a circular rotation along the filter residue collecting tank; In order to facilitate the uninterrupted cleaning of the impurities filtered out of the filter residue bed, and sequentially discharge through the filter residue outlet and the residue discharge channel, to prevent impurities from accumulating in the filter residue collection tank and affecting the filtering effect.

One end of the rotating shaft is located in the recovery bed, and the other end is connected with the rotating wheel after passing through the filter residue bed, and the central axes of the rotating shaft, the filter residue bed and the recovery bed are coincident, and the function of the rotating wheel is to rotate The shaft provides driving force, and the filter residue collection tank is used to collect impurities filtered out from microalgal cells and culture fluid.

Preferably, the filter residue discharge port is one or more openings of suitable shape, so as to facilitate the discharge of impurities to designated areas.

More preferably, the filter residue outlet is two circular holes, symmetrically distributed on the bottom surface of the filter residue collection tank, which can effectively improve the discharge speed and efficiency of the filter residue.

Further, the recovery system also includes a cleaning water spray pipe, the cleaning water spray pipe is located in the recovery bed, the cleaning water spray pipe is provided with a water spray hole, and the jet flow direction of the water spray hole faces the recovery bed, and It is at an oblique angle to the surface of the recovery bed or the flow direction of the microalgae cells. The cleaning water spray pipe is mainly used for cleaning and scouring the nutrient salts, bacterial groups and other foreign matters on the surface of the microalgae cells on the surface of the recovery bed. Close the bed.

Preferably, the inclination angle between the jet flow direction of the water spray hole and the recovery bed surface or the flow direction of the microalgae cells is 45°-90°.

Further, the harvesting system also includes a clean water tank, which is arranged outside the harvesting system, and the clean water tank is connected to one end of the cleaning water spray pipe, and the other end of the clean water spray pipe is located in the recovery bed, and the clean water The clean water in the tank is salt-free or salt-containing clean water with the same concentration as the culture medium. When harvesting algae cells containing glycerol glucoside, it is necessary to use salt-containing clean water with the same concentration as the culture medium as a cleaning solution to prevent algal contamination. Intracellular glycerol glucoside is secreted into the washing solution, resulting in loss of glycerol glucoside.

Further, the rotating shaft is a hollow structure, one end of the rotating shaft is located inside the recovery bed, the other end passes through the slag bed and connects with the rotating wheel and then communicates with the clean water tank, and one or both ends of the cleaning water spray pipe are It communicates with the part of the rotating shaft located in the recovery bed, and the cleaning water spray pipe can rotate together with the rotating shaft, and sprays cleaning water to the surface of the recovery bed.

Further, the harvesting system also includes a support frame, a clean water pump, and a clean water input port, the clean water tank is fixed on the support frame, the clean water tank is provided with a clean water input port, and a dynamic sealing part connected, the clean water input port is connected with the clean water pump.

Preferably, the dynamic sealing part includes a dynamic sealing structure of packing seal and mechanical seal.

Further, the harvesting system also includes a culture fluid collection tank, the culture fluid collection tank is arranged at the lower part of the bed surface of the recovery bed, and is used to collect the liquid filtered from the recovery bed, and the lower end of the recovery bed forms a The algae mud output port runs through the bottom surface of the culture solution collection tank, and the part where the algae mud output port contacts the bottom surface of the culture solution collection tank is sealed and connected to prevent the culture solution in the culture solution collection tank from leaking and mixing with the microalgae cells again.

The microalgae cells and culture fluid that have been filtered out of impurities through the filter residue bed enter the bed surface of the recovery bed. During the flow process, the culture fluid and microalgae cells are quickly separated, and the microalgae cells are collected and discharged from the algae mud output port at the bottom of the recovery bed , while the culture solution enters the culture solution collection tank through the membrane hole filter structure on the harvesting bed bed.

Further, the lower part of the culture fluid collection tank is provided with a waste liquid outlet, which is convenient for collecting and discharging the culture fluid in the culture fluid collection tank.

Further, the cultivation-harvesting integrated system also includes a liquid delivery pump, a liquid inlet valve, and the cultivation system, the liquid delivery pump, the liquid inlet valve, and a liquid inlet pipe are connected in sequence.

Further, the cultivation-harvesting integrated system also includes a backflow pump and a liquid outlet valve, and the waste liquid outlet, the backflow pump, and the liquid outlet valve are connected in sequence to discharge the collected waste liquid after treatment, and separate The final microalgal cells are used for the next step.

Secondly, the present invention provides a kind of cultivation-harvesting method of microalgae cell, comprises the steps:

(1) At first, carry out photosynthetic culture to microalgae cell by culture system, then microalgae cell and culture fluid are distributed to the bed surface of filter residue bed, through the filtration structure of the bed surface of filter residue bed, the microalgae cell and culture fluid in Impurities are filtered;

(2) After step (1), the microalgae cells and culture fluid filtered out of impurities enter the bed surface of the recovery bed, so that the culture fluid and microalgae cells are quickly separated, and the microalgae cells are collected and output from the algae mud at the bottom of the recovery bed Discharge in the mouth, that's it.

Preferably, in the method for culturing-harvesting microalgae cells, the microalgae cells are spirulina.

At last, the present invention provides a kind of cultivation-harvesting method for the spirulina of synthetic glycerol glucoside (GG), comprising the steps:

(1) First, make the liquid delivery pump, the liquid inlet valve, the reflux pump, and the liquid outlet valve all in the closed state, then insert the culture medium and spirulina in the photoreactor;

(2) Start the agitator and the light source, and feed the carbon source gas through the intake pipe. The spirulina absorbs the carbon source under photosynthesis and generates oxygen, which is released into the culture medium. With the agitation of the agitator, the oxygen leaves the culture medium , discharged from the exhaust port to the outside of the photoreactor;

(3) After the concentration of spirulina reaches the set value, stop the introduction of light and carbon source gas, open the liquid delivery pump, liquid inlet valve, return pump, and liquid outlet valve, and the spirulina and culture solution pass through the liquid delivery pump, The liquid inlet valve enters the liquid inlet pipe, and the liquid inlet pipe evenly distributes the spirulina and culture solution to the bed surface of the filter residue bed through the liquid distribution hole, and filters the impurities in the spirulina and culture solution through its membrane filter structure, and the impurities are collected in the After the filter residue is collected in the tank, it is removed from the filter residue outlet by the filter residue cleaning rod and enters the filter residue channel, and finally the recovery system is cleaned out, and the spirulina and culture solution that have filtered out impurities enter the recovery bed;

(4) The spirulina and culture fluid filtered out of impurities in step (5) enter the recovery bed surface, meanwhile, the saline clean water with the concentration equal to the culture fluid provided in the clean water tank enters the cleaning spray after passing through the hollow rotating shaft. Water pipe, with the rotation of the rotating shaft, after the clean water is sprayed from the water spray hole of the cleaning water spray pipe, the spirulina on the recovery bed is cleaned, and the jet flow direction of the water spray hole is consistent with the flow direction of the spirulina. The direction is at an inclination angle of 45°-90°, so that the culture medium and spirulina can be separated quickly. After the spirulina is collected, it is discharged from the algae mud output port at the bottom of the harvesting bed; while the culture medium enters the culture medium through the membrane pore filter structure on the bed surface. After the liquid collection tank, it enters the return pump from the waste liquid discharge port, and then passes through the liquid outlet valve and is used again for the cultivation of Spirulina.

Compared with prior art, the beneficial effect that the present invention obtains is:

(1) The present invention integrates the design of the intake pipe and the agitator of the cultivation system, the agitator is provided with an air inlet pipe and an air outlet hole, and after the carbon-containing source gas passes through the air inlet pipe, it enters the culture solution from the air outlet hole, This setting can achieve aeration while stirring, so that the incoming gas can be more evenly mixed into the culture solution, so that the microalgae cells in the photoreactor can receive light, nutrient supply, and carbon source gas absorption more uniformly.

(2) Compared with the traditional recovery bed, the present invention adopts the upper and lower layered structure of the filter residue bed and the recovery bed, which saves the floor area for equipment layout, and the large particle impurities filtered out by the filter residue bed are cleaned out in time through the cleaning rod, The capacity of the device to process waste has been improved. In addition, the bed surface structure of the recovery bed adopts a funnel-shaped structure with a variable curved surface whose inclination angle changes continuously or stepwise from 0-90°. It is relatively slow, and the separation area of the bed surface is large, which is conducive to the rapid separation of a large amount of culture medium. With the rapid separation of the culture medium, the harvesting bed surface shrinks rapidly, and the inclination angle of the bed surface also increases continuously, which is conducive to the flow of microalgae cells And rapid discharge of the recovery bed, preventing microalgae cell accumulation problems, thereby greatly improving the recovery efficiency.

(3) The microalgae cell culture-harvesting integrated system of the present invention is very suitable for the extraction of glycerol glucoside in spirulina. During the cultivation process, spirulina absorbs carbon dioxide through photosynthesis and generates oxygen, which is released into the liquid medium , along with ventilation and stirring, oxygen leaves the liquid layer and is discharged from the exhaust port to the outside of the photoreactor, which ensures the normal growth of Spirulina and the accumulation of glycerol glucoside in the algae cells. After the cultivation is completed, the When the spirulina cells containing glycerol glucoside are harvested, the algae cells and the culture medium can be quickly separated to realize efficient cultivation and harvesting of the spirulina cells.

Description of drawings

The accompanying drawings constituting a part of the present application are used to provide further understanding of the present application, and the schematic embodiments and descriptions of the present application are used to explain the present application, and do not constitute improper limitations to the present application.

Fig. 1 is the culture-harvesting integrated system of microalgal cells of the present invention.

Fig. 2 is a structural schematic diagram of the harvesting system of the present invention.

Fig. 3 is the structure diagram of filter residue bed and recovery bed in embodiment 1.

Fig. 4 is the structural representation of filter residue bed and recovery bed in embodiment 2.

Fig. 5 is a schematic diagram of the sealing connection between the clean water tank and the rotating shaft according to the present invention.

The symbols in the drawings represent: 1-photoreactor, 2-microalgae cells, 3-culture solution, 4-intake pipe, 5-carbon source gas, 6-stirrer, 7-exhaust port, 8-light source , 9-recovery system, 10-liquid delivery pump, 11-liquid inlet valve, 12-reflux pump, 13-liquid outlet valve, 14-liquid inlet pipe, 15-residue bed, 16-recovery bed, 17-cleaning Water spray pipe, 18-culture solution collection tank, 19-algae mud output port, 20-waste liquid discharge port, 21-clean water pump, 22-filter residue collection tank, 23-filter residue cleaning rod, 24-clean water tank, 25-rotation wheel, 26-residue discharge outlet, 27-rotation shaft, 28-support frame, 29-dynamic sealing part, 30-clean water input port, 31-slag discharge channel.

Detailed ways

It should be pointed out that the following detailed description is exemplary and intended to provide further explanation to the present application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.

It should be noted that the terminology used here is only for describing specific implementations, and is not intended to limit the exemplary implementations according to the present application. As used herein, unless the context clearly dictates otherwise, the singular is intended to include the plural, and it should also be understood that when the terms "comprising" and/or "comprising" are used in this specification, they mean There are features, steps, operations, means, components and/or combinations thereof.

As introduced in the background technology, the existing methods and systems for culturing and harvesting microalgae cells still have problems such as complex cultivation process, high cost, and low harvesting efficiency. Therefore, the present invention proposes a microalgae cell culture - The harvesting integrated system and method, the present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

Example 1

As shown in Figures 1-5, a culture-harvesting integrated system for microalgae cells includes a culture system, a harvesting system 9, a liquid delivery pump 10, an inlet valve 11, a return pump 12, and an outlet valve 13; The cultivation system is used for culturing algae cells, and the harvesting system 9 is used for separating microalgae cells and culture fluid.

The culture system includes: a photoreactor 1 , an air inlet pipe 4 , an agitator 6 , an exhaust port 7 , and a light source 8 .

The microalgae cells 2 and the culture solution 3 are mixed together and located in the photoreactor 1. The photoreactor 1 is cylindrical. The source is connected, and the carbon-containing source gas 5 enters the culture solution 3 through the inlet pipe 4 to provide carbon elements for the photosynthetic growth of microalgae cells.

The agitator 6 is arranged in the photoreactor 1 , the exhaust port 7 is arranged on the upper part of the culture medium, and the light source 8 is an LED arranged inside the photoreactor 1 .

The recovery system includes: a liquid inlet pipe 14, a filter residue bed 15, and a recovery bed 16, the liquid delivery pump 10, the liquid inlet valve 11, and the liquid inlet pipe 14 are connected in sequence, and the diameter of the lower end surface of the filter residue bed 15 is larger than A truncated conical structure with a diameter of the upper end surface, the side of which forms the bed surface of the filter structure, and the liquid inlet pipe 14 is fixed on the upper end of the side of the truncated cone in a ring shape, and the liquid inlet pipe 14 is provided with a liquid distribution hole whose jet direction faces the filter residue bed ; The bed surface of the filter residue bed 15 is a membrane pore filter structure, which is made of soft filter cloth with 50 mesh pores.

Described filter residue bed 15 is arranged on the top of recovery bed 16, and the lower end of recovery bed 16 forms algae mud outlet 19; A filter residue discharge port 26 is provided, and a residue discharge channel 31 is connected to the filter residue discharge port 26 .

The filter residue collecting tank 22 is provided with a filter residue cleaning rod 23 which is connected with the rotating shaft 27 and can rotate along the filter residue collecting tank 22 under the drive of the rotating shaft 27 .

The recovery bed 16 is a funnel-shaped structure, and its side wall forms a bed surface of a filter structure. The inclination angle of the bed surface changes continuously from 0 to 90°, and the microalgae cells 2 and culture solution 3 of impurities are filtered out through the filter residue bed 15. Enter the bed surface of the recovery bed 16, during the flow process, the culture solution and the microalgae cells 2 are separated rapidly, and the algae mud formed after the collection of the microalgae cells 2 is discharged from the algae mud output port 19 at the bottom of the recovery bed 16, and the cultivation The liquid 3 enters the culture solution collection tank 18 through the membrane pore filter structure on the bed surface, and the bed surface of the recovery bed 16 is a membrane pore filter structure made of hard filter cloth with 300 mesh pores.

The water spray cleaning pipe 17 is arranged in the recovery bed 16, the rotating shaft 27 is a hollow structure, one end of the rotating shaft 27 is located in the recovery bed 16, and the other end passes through the filter residue bed 15 and connects with the rotating wheel 25 connected, and the central axes of the rotating shaft 27, the filter residue bed 15, and the recovery bed 16 coincide, the water spray cleaning pipe 4 communicates with the part where the rotating shaft 27 extends into the recovery bed 16, and the water spray cleaning pipe 17 is provided with There are some water spray holes, and the jet flow direction of the water spray holes faces the recovery bed 3, and is at an angle of 45° to the flow direction of the microalgae cells; Above the rotating shaft 27 and fixed on the support frame 28, the clean water tank 24 is provided with a clean water input port 30, the rotating shaft 27 and the clean water tank 24 are connected by a dynamic sealing part 29, and the clean water input port 30 is connected to the clean water tank 24. The water pump 21 is connected, and the dynamic sealing part 29 is a packing-sealed dynamic sealing structure.

The rotating wheel 25 is sleeved on the rotating shaft 27 to provide driving force for the rotation of the rotating shaft 27. The clear water in the clean water tank 24 enters the cleaning water spray pipe 17 after passing through the hollow rotating shaft 27, and with the rotation of the rotating shaft 27 After clear water is sprayed from the spray hole of the cleaning spray pipe 17, the microalgae cells on the bed surface of the recovery bed 16 are evenly cleaned, and when the microalgae cells are cleaned, the microalgae cells are washed into the algae mud An output port 19 exits the recovery bed 16 .

The cleaning water spray pipe 17 is provided with several water spray holes, and the jet flow direction of the water spray holes faces the recovery bed 16, and forms an inclined angle of 45° with the direction of algae mud flow.

Described culture fluid collection tank 18 is arranged on the bed surface bottom of recovery bed 16, and the algae mud outlet 19 that the lower end of recovery bed 16 forms runs through the bottom surface of culture fluid collection tank 18, and algae mud output port 19 is collected with culture fluid. The parts where the bottom surface of the groove 18 contacts are sealed and connected.

The bottom of the culture solution collection tank 18 is provided with a waste liquid discharge port 20, and the waste liquid discharge port 20 is connected with the reflux pump 12 and the liquid outlet valve 13, so as to discharge the collected waste liquid after treatment, and the separated microalgae Cells are used for further processing.

Example 2

A microalgae harvesting system, the same as in Example 1, differs in that: (1) the bed surface of the filter residue bed 15 is made of a hard membrane material with membrane pores of 80 mesh.

(2) The bed surface of the recovery bed 16 is made of a soft filter cloth with membrane holes of 100 mesh.

(3) The inclination angle α gradient of the bed surface of the recovery bed 16 relative to the horizontal plane is divided into 4 gradients, which are α ₁ =5°, α ₂ =15°, α ₃ =45°, α ₄ =85 °.

Example 3

An integrated system for cultivating and harvesting microalgae cells is the same as in Example 1, the difference is that: (1) the bed surface of the filter residue bed 15 is made of a hard membrane material with a membrane hole of 30 meshes.

(2) The bed surface of the recovery bed 16 is made of soft filter cloth with membrane holes of 400 mesh.

(3) The jet flow direction of the water spray holes on the cleaning water spray pipe 17 is at an angle of 90° to the flow direction of the algae mud. The light source 8 is natural sunlight.

(4) The dynamic sealing part 29 is a dynamic sealing structure of a mechanical seal.

(5) The integrated design of the inlet pipe and the agitator: the agitator is provided with an inlet pipe and an air outlet, and the carbon-containing source gas 5 enters the culture solution 3 through the air outlet after passing through the inlet pipe.

Example 4

An integrated system for cultivating and harvesting microalgae cells is the same as in Example 1, except that: (1) the bed surface of the filter residue bed 15 is made of a soft filter cloth material with membrane pores of 100 meshes.

(2) The bed surface of the recovery bed 16 is made of soft filter cloth with membrane holes of 600 mesh.

Example 5

A method of utilizing integrated system culture in embodiment 1-harvesting can synthesize the spirulina of glycerol glucoside (GG), comprises the steps:

(1) First, make the liquid delivery pump 10, the liquid inlet valve 11, the return pump 12, and the liquid outlet valve 13 all in a closed state, then insert the culture solution 3 and the spirulina 2 in the photoreactor 1; 3 is a f/2 culture solution, and the culture solution 3 includes nutrients such as water, nitrogen, phosphorus, calcium, magnesium, iron and trace metal nutrient salts, and the carbon-containing source gas is a gas containing CO ₂ ;

(2) start agitator 6 and light source 8, pass into _CO gas 5 by inlet pipe 4, spirulina 2 has possessed the basic condition that can photosynthesis grow and synthesize GG in spirulina, then start the synthesis of GG, GG generation process Among them, Spirulina 2 absorbs carbon dioxide under photosynthesis and generates oxygen, which is released into the culture solution 3. With the agitation of the stirrer, the oxygen leaves the culture solution 3 and is discharged from the exhaust port 7 to the outside of the photoreactor 1. To ensure the smooth progress of the synthesis process of GG;

(3) As the GG synthesis reaction continues, the GG content in Spirulina 2 increases gradually. (3) After the content of Spirulina 2 in the culture solution 3 reaches 2g/L, stop the light and the passage of the carbon source gas 5. In, open the liquid delivery pump 10, the liquid inlet valve 11, the return pump 12, and the liquid outlet valve 13, so that the spirulina 2 and the culture solution 3 in the photoreactor 1 enter the liquid inlet pipe 14 through the liquid inlet valve 11, and the liquid inlet pipe 1. Evenly distribute the Spirulina 2 and the culture solution 3 to the side of the filter residue bed 15 through the liquid distribution hole on the liquid inlet pipe 14, and filter the impurities in the Spirulina 2 and the culture solution 3 through its membrane pore filtration structure, and the impurities are collected After being in the filter residue collection tank 22, it is removed from the filter residue discharge port 26 by the filter residue cleaning rod 23, and the spirulina 2 and culture solution 3 that filter out impurities enter the recovery bed;

(4) the spirulina 2 and the nutrient solution 3 that filter out impurities through step (5) enter the bed surface of the recovery bed 16, and meanwhile, the clear water in the clean water tank 24 enters the cleaning water spray pipe 17 after passing through the hollow rotating shaft 25, Along with the rotation of rotating shaft 25, after clear water is sprayed from the spray hole of cleaning water spray pipe 17, the spirulina on the bed surface of recovery bed 16 is cleaned evenly, and the jet flow direction of water spray hole and spirulina flow The direction of the spirulina is 45°-90° inclined angle, so that the culture medium 3 and the spirulina 2 are separated rapidly, and the spirulina 2 is collected and discharged from the algae mud output port 19 at the bottom of the recovery bed 16 for the next step of separation and extraction of GG , purification; and the culture solution 3 base enters the culture solution collection tank 18 through the membrane hole filter structure on the bed surface and enters the return pump 12 from the waste liquid discharge port 20, and then passes through the liquid outlet valve 13 and is used again for the production of the spirulina 2 nourish.

The above descriptions are only preferred embodiments of the present application, and are not intended to limit the present application. For those skilled in the art, there may be various modifications and changes in the present application. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of this application shall be included within the protection scope of this application.

Claims (10)

1. a kind of culture of microalgae cell-harvesting integral system, it is characterised in that：The integral system by culture systems, Recovery system is constituted；The culture systems include：Photoreactor, air inlet pipe, blender, light source；

In the culture solution of one end insertion Photoreactor of the air inlet pipe；The blender is arranged in Photoreactor；The light Photoreactor is arranged in internally and/or externally in source；

The recovery system is made of filter residue bed, harvesting bed, and the setting of filter residue bed is above harvesting bed, and the central axis weight of the two It closes, microalgae cell enters filter residue bed after completing culture in Photoreactor；The lower end for harvesting bed forms algal gel delivery outlet；The filter Slag-bed is the circular cone shape structure that lower end surface diameter is greater than upper surface diameter, and the side of the frustum of a cone forms the filter residue bed of filter structure Bed surface；The harvesting bed is plane of bending loudspeaker funnel type structure, and side wall forms the harvesting bed bed surface of filter structure, harvests bed bed The inclined angle alpha of face with respect to the horizontal plane is from 0 ° to 90 ° of consecutive variations or change of gradient.

2. the culture of microalgae cell as described in claim 1-harvesting integral system, it is characterised in that：The air inlet pipe with Blender integrated design：Admission line and venthole are provided in blender, after carbon containing source gas passes through admission line, then from Enter in culture solution in venthole；

Or, the culture systems further include exhaust outlet, culture solution ullage is arranged in Photoreactor in the exhaust outlet.

3. the culture of microalgae cell as described in claim 1-harvesting integral system, it is characterised in that：The Photoreactor Including closed tubular reactor, flat-plate reactor, column type Photoreactor or open raceway pond；

Or, the shape of the blender includes spiral-shaped, quant shape；

Or, the light source includes nature sunlight, artificial light source；Preferably, the artificial light source includes LED, fluorescent lamp, mercury Lamp.

4. the culture of microalgae cell as described in any one of claims 1-3-harvesting integral system, it is characterised in that：It is described Recovery system further includes inlet tube, and inlet tube is connect with culture systems, and inlet tube is fixed on the upper end of filter residue bed bed surface in a ring, And liquid distributing hole of several jet directions towards filter residue bed is provided on inlet tube；

Or, the filter residue bed bed surface is fenestra filter structure, the fenestra filter structure is by the hard of fenestra 10-100 mesh or soft Matter filtering material is made；

Or, the harvesting bed bed surface is fenestra filter structure, the fenestra filter structure is by the hard of fenestra 100-800 mesh or soft Matter filtering material is made.

5. the culture of microalgae cell as claimed in claim 4-harvesting integral system, it is characterised in that：The recovery system It further include residue collection slot, filter residue cleaning bar, rotating wheel, sludge outlet, slagging channel, rotation axis；The residue collection slot The periphery of the lower end surface of filter residue bed is set, and is closely connect with filter residue bed, the sludge outlet is arranged in residue collection slot In, slagging channel is connect with sludge outlet, the filter residue cleaning bar and rotation axis connection, and can be under the driving of rotation axis Circular-rotation is done along residue collection slot, one end of the rotation axis is located in harvesting bed, and the other end is after filter residue bed and turns Driving wheel connection, and rotation axis, filter residue bed, the central axis coincidence for harvesting bed three.

6. the culture of microalgae cell as claimed in claim 5-harvesting integral system, it is characterised in that：The recovery system It further include cleaning sparge pipe, the cleaning sparge pipe is located in harvesting bed, and it cleans on sparge pipe and is provided with hole for water spraying, hole for water spraying Jet direction towards harvesting bed, and the jet direction of hole for water spraying and harvesting bed bed surface or microalgae cell flowing the inclined angle in direction Degree, it is preferred that the inclination angle is 45 ° -90 °；

Or, the recovery system further includes filtered water tank, filtered water tank is arranged outside recovery system, and filtered water tank and cleaning sparge pipe One end connection, clean sparge pipe the other end be located at harvesting bed in.

7. the culture of microalgae cell as claimed in claim 6-harvesting integral system, it is characterised in that：The recovery system It further include dynamic sealing component, and the rotation axis is hollow structure, one end of rotation axis is located inside harvesting bed, and the other end passes through Filter residue bed is connected to filtered water tank again after connecting with rotating wheel, and the one or both ends of the cleaning sparge pipe, which are located at rotation axis, adopts The part connection in bed is received, and can be rotated together with rotation axis；

Preferably, the recovery system further includes support frame, fresh water pump, clear water input port, and the filtered water tank is fixed on support frame On, clear water input port is arranged on filtered water tank, and fresh water pump is connect with clear water input port；

Preferably, the dynamic sealing component includes the movable sealing structure of packing seal, mechanical seal.

8. the culture of microalgae cell as claimed in claim 7-harvesting integral system, it is characterised in that：The recovery system It further include culture solution collecting tank, the bed surface lower part of harvesting bed is arranged in the culture solution collecting tank, harvests the lower end formation of bed Algal gel delivery outlet runs through the bottom surface of culture solution collecting tank, and algal gel delivery outlet is collected the position that groove bottom contacts with culture solution and sealed Connection；

Preferably, the lower part of the culture solution collecting tank is provided with waste liquid outlet；

It is further preferred that the integral system further includes liquid-feeding pump, liquid flowing valve, reflux pump, liquid valve door, the culture System, liquid-feeding pump, liquid flowing valve, inlet tube are sequentially connected, and waste liquid outlet, reflux pump, liquid valve door are sequentially connected；

Or, the inclined angle alpha echelon variation of the harvesting bed bed surface with respect to the horizontal plane is divided into 4 gradients：5°,15°,45°,85°.

9. carrying out culture-harvesting of microalgae cell using the described in any item cultures of such as claim 1-8-harvesting integral system Method, it is characterised in that：Include the following steps：

(1) firstly, carrying out photosynthetic culture to microalgae cell by culture systems, microalgae cell and culture solution are then distributed to filter On slag-bed bed surface, the impurity in microalgae cell and culture solution is filtered by the filter structure of filter residue bed bed surface；

(2) deimpurity microalgae cell is filtered by step (1) and culture solution enters the bed surface of harvesting bed, keep culture solution and microalgae thin Born of the same parents' quick separating, microalgae cell are discharged from the algal gel delivery outlet of harvesting bed bottom after collecting；

Preferably, the microalgae cell is spirulina.

10. capableing of the side of the spirulina of synthetic glycerine glucoside using integral system culture-harvesting as claimed in claim 7 Method, spy are：Include the following steps：

(1) firstly, making liquid-feeding pump, liquid flowing valve, reflux pump, liquid valve door be in closed state, then in Photoreactor Access culture solution and spirulina；

(2) start blender and light source, carbon-source gas is passed through by air inlet pipe, spirulina absorbs carbon source and life under photosynthesis It at oxygen, discharges to culture solution, with the stirring action of blender, oxygen is detached from liquid culture solution, is expelled to light from exhaust outlet Outside reactor；

(3) after spiral concentration of algae reaches setting value, stop being passed through for illumination and carbon-source gas, open liquid-feeding pump, liquid flowing valve, Reflux pump, liquid valve door, spirulina and culture solution pass sequentially through liquid-feeding pump, liquid flowing valve enters inlet tube, and inlet tube is by spiral Algae and culture solution are evenly distributed to filter residue bed bed surface by liquid distributing hole, by its fenestra filter structure in spirulina and culture solution Impurity be filtered, after impurity collects in residue collection slot, removed from sludge outlet by filter residue cleaning bar into filter Slag channel finally cleans out recovery system, and filters out the spirulina of impurity and culture solution enters harvesting bed；

(4) deimpurity spirulina is filtered by step (5) and culture solution enters harvesting bed bed surface, meanwhile, it is arranged in filtered water tank With the saliferous clean water of culture solution isoconcentration by entering cleaning sparge pipe after hollow rotation axis, with the rotation of rotation axis, After clean water is sprayed from the hole for water spraying of cleaning sparge pipe, the spirulina on harvesting bed bed surface is cleaned, and hole for water spraying is penetrated The direction for flowing direction and spirulina flowing is in 45 ° of -90 ° of tilt angles, makes culture solution and spirulina quick separating, spirulina collects It is discharged from the algal gel delivery outlet of harvesting bed bottom afterwards；And culture solution then enters culture solution by the fenestra filter structure on bed surface Enter reflux pump from waste liquid outlet after collecting tank, is then re-used for the culture of spirulina behind the door by liquid valve；

Preferably, the carbon containing source gas is containing CO₂Gas；

Or, the culture solution is seawater culture solution, preferably f/2 culture solution；

Or, the culture solution is fresh water culture solution, preferably BG；

Or, the culture solution is the acid culture solution by improvement；

Or, the culture solution is by the alkaline culture solution of improvement, preferably Zarrok alkalinity culture solution；

Or, the culture solution is MC green alga culture solution；

Preferably, the nutriment in the culture solution includes water, nitrogen, phosphorus, calcium, magnesium, iron and trace meter nutritive salt.