CN108676702A - A kind of extraction element and its operating method - Google Patents
A kind of extraction element and its operating method Download PDFInfo
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- CN108676702A CN108676702A CN201810502244.8A CN201810502244A CN108676702A CN 108676702 A CN108676702 A CN 108676702A CN 201810502244 A CN201810502244 A CN 201810502244A CN 108676702 A CN108676702 A CN 108676702A
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- 238000000605 extraction Methods 0.000 title claims abstract description 47
- 238000011017 operating method Methods 0.000 title claims abstract description 10
- 238000005406 washing Methods 0.000 claims abstract description 86
- 239000007788 liquid Substances 0.000 claims abstract description 52
- 239000002699 waste material Substances 0.000 claims abstract description 39
- 210000000577 adipose tissue Anatomy 0.000 claims abstract description 34
- 238000000926 separation method Methods 0.000 claims abstract description 23
- 238000007443 liposuction Methods 0.000 claims abstract description 22
- 239000007924 injection Substances 0.000 claims abstract description 19
- 238000002347 injection Methods 0.000 claims abstract description 19
- 238000000034 method Methods 0.000 claims abstract description 15
- 239000000284 extract Substances 0.000 claims abstract description 13
- 230000008569 process Effects 0.000 claims abstract description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000002504 physiological saline solution Substances 0.000 claims description 23
- 238000009434 installation Methods 0.000 claims description 21
- 239000011159 matrix material Substances 0.000 claims description 17
- 239000012530 fluid Substances 0.000 claims description 9
- 239000012535 impurity Substances 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 5
- 230000035479 physiological effects, processes and functions Effects 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 claims description 2
- 239000000243 solution Substances 0.000 abstract description 15
- 230000004083 survival effect Effects 0.000 abstract description 5
- 238000004140 cleaning Methods 0.000 abstract description 4
- 239000011780 sodium chloride Substances 0.000 abstract description 3
- 239000002351 wastewater Substances 0.000 abstract 1
- 238000009830 intercalation Methods 0.000 description 10
- 230000002687 intercalation Effects 0.000 description 10
- 238000001125 extrusion Methods 0.000 description 9
- 230000000694 effects Effects 0.000 description 7
- 210000000130 stem cell Anatomy 0.000 description 7
- 239000000758 substrate Substances 0.000 description 7
- 239000002775 capsule Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 6
- 238000007789 sealing Methods 0.000 description 4
- 239000004519 grease Substances 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 210000000988 bone and bone Anatomy 0.000 description 2
- 239000012267 brine Substances 0.000 description 2
- 230000004069 differentiation Effects 0.000 description 2
- 239000002360 explosive Substances 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 2
- 210000001519 tissue Anatomy 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 241000283216 Phocidae Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 238000004026 adhesive bonding Methods 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000033115 angiogenesis Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 210000003038 endothelium Anatomy 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 238000002513 implantation Methods 0.000 description 1
- 238000011900 installation process Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229940127215 low-molecular weight heparin Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 230000003076 paracrine Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000000250 revascularization Effects 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000009288 screen filtration Methods 0.000 description 1
- 239000000565 sealant Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0662—Stem cells
- C12N5/0667—Adipose-derived stem cells [ADSC]; Adipose stromal stem cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
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- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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Abstract
The invention discloses a kind of extraction element and its operating methods, plug including washing cylinder and mounted on washing cylinder both ends, it is provided on the plug multiple for adipose tissue, the fatty mouth of injection and the extraction of liposuction liquid and the pipeline fill assembly for saline injection, the waste liquid discharge component for the waste water rinsed to be discharged and the functional unit for assisting adipose tissue extract function are provided on the plug, it is provided with filter screen on the liposuction head, the filter screen includes and the fixed mounting plate of liposuction, several openings to match with fatty mouth are offered on the mounting plate, it is provided with strainer in the opening.The separation of adipose tissue carries out in a closed container in the whole process, and need not add cleaning solution, and cell survival rate is relatively high, and the time of cell separation is short.
Description
Technical field
The present invention relates to a kind of medical instruments, more specifically, it relates to a kind of extraction element and its operating method.
Background technology
It can be divided into fat, bone, soft with the stem cell of isolated Multidirectional Differentiation in body fat suspension
The cells such as bone, muscle, blood vessel endothelium, liver, spleen, nerve, and this adult with differentiation potential obtained from body fat
Stem cell is referred to as human adipose-derived stem cell, it has the advantage that for other stem cells：
1, abundance, materials are easy；
2, it is easy to expand, is not easy aging；
3, have the function of paracrine, the correlation factor for promoting angiogenesis can be secreted, promote revascularization；
It will not cause strong rejection when 4, transplanting.
Publication No. discloses one kind separation people's fat from human fat tissue for the Chinese patent of CN104357387A and does carefully
The method of born of the same parents first passes through and adds a small amount of Low-molecular-weight Heparins Calcium in physiological saline, as cleaning solution, by remaining red blood cell
Equal impurity effectively remove, and then isolate the high human adipose-derived stem cell of purity, but this method is during the treatment, needs
It adds other cleaning solutions to be cleaned, and by centrifuging, allows the purity of fat stem cell to improve, but need to pass through centrifugation
Separation, and cultivated, speed is slower in the whole process, and cell survival rate is relatively low.
Invention content
Problem in view of the prior art, the present invention provide a kind of extraction element, extract the process time section of separation, cell
Survival rate is high.
To achieve the above object, the present invention provides following technical solutions：
A kind of extraction element, including, cylinder is washed, washing point is carried out for storing liposuction liquid, and to the extract
From；Plug is separately mounted to the both ends of washing cylinder, the closing for washing cylinder；Fatty mouth, offers several,
And be respectively arranged on plug, it is connected to washing cylinder inside；Filter screen is respectively placed between plug and washing cylinder,
It is corresponding with fatty mouth, it is used for the filtering of adipose tissue；It is provided with interface on the plug, and washs the stifled of cylinder both ends
It is connected separately with pipeline fill assembly on head and physiological saline is injected washing separation by waste liquid discharge component, the fill assembly
Cylinder carries out through waste liquid discharge component discharge after washing separation liposuction liquid, and liposuction liquid is after washing detaches through filter screen
It is extracted out by fatty mouth after filtering.
Through the above technical solutions, pipeline fill assembly saline injection, enter in washing cylinder, and from waste liquid
Discharge component is discharged, and is injected liposuction liquid by fatty mouth and is taken away impurity in the flow process of physiological saline,
And adipose tissue is suspended in liquid surface, achievees the effect that separation, furthermore it is possible to by filter screen, adipose tissue is allowed to carry out
Filter, it is easy to use so as to obtain the adipose tissue block of certain scale.In addition, in this process, can pipeline be injected group
Part and waste liquid discharge component are closed, and are washed cylinder by shaking and can mutually be collided by adipose tissue, are allowed
Adipose tissue can be detached further, be discharged by the impurity isolated, and the higher adipose tissue of purity is obtained, and
And the adipose tissue after processing can be extracted by fatty mouth.And the separation of adipose tissue is close at one in the whole process
It is carried out in the container closed, and cleaning solution need not be added, cell survival rate is relatively high, and the time of cell separation is short.
The present invention is further arranged to：The plug includes matrix and the end cap on matrix, described matrix with
Washing cylinder is fixedly connected, and the end cap is located on matrix, and the fat mouth may be contained with interface on matrix, described
Filter screen is mounted on matrix, and filter screen setting corresponding with fatty mouth.
Through the above technical solutions, flow in pipes and interface grafting, make its installation more convenient, and use L shape knots
Its flow in pipes can be extended out from side for the interface of structure, and then will not interfere between pipeline and fatty mouth, using than
It is more convenient.
The present invention is further arranged to：The filter screen includes mounting plate and opens up opening on a mounting board, described
Strainer is provided in opening, the strainer is oppositely arranged with fatty mouth.
Through the above technical solutions, the setting of switch module, can allow between the branch line portion and straight line portion of connection-peg can
Break-make is carried out, have the function that flow in pipes and washs the break-make between cylinder.
The present invention is further arranged to：Open amount setting equal with fatty mouth quantity.
Through the above technical solutions, catch bar pushes movable head in straight line portion internal slide, and then it can be obstructed directly
Line portion and branch line portion, achieve the purpose that closing and unlatching.
The present invention is further arranged to：Conical opening is provided in described matrix, the conical opening is connected to fatty mouth.
Through the above technical solutions, threaded sleeve rod rotates, screw rod is rotatablely connected with threaded sleeve rod, allows it that can drive screw rod
It slides axially, and then pulls movable head, realize iris action of the movable head between branch line portion and straight line portion, and limit knot
The setting of structure can allow screw rod to be limited circumferentially rotating direction.
The present invention is further arranged to：The washing cylinder is provided at both ends with link slot, and the plug is socketed in respectively
On link slot, and plug and washing cylinder sealed set.
Through the above technical solutions, the setting of link slot allows and liposuction head and discharge head and washs the installation side of cylinder
Just, the position of connection is relatively more fixed, and then is more prone to during Assembling Production.
The present invention is further arranged to：It is provided with several extrusion balls in the washing cylinder
Another mesh of the present invention is to provide for a kind of operating method, is used by above-mentioned apparatus, can allow entire behaviour
That makees is time-consuming few, and cell survival rate is high.
A kind of operating method of extraction element described above, includes the following steps：
S1, pipeline connection, connect normal saline bag, discharge line connects waste fluid bag by flow in pipes；
Washing cylinder is inverted by S2, air-discharging, allows waste liquid discharge component to be placed in above level, by the life in normal saline bag
Reason brine is injected into washing cylinder, will be washed the air in cylinder and is discharged；
S3, primary wash are just being set washing cylinder overturning, and waste liquid discharge component is placed in horizontal down, from a wherein fat
Fat mouth injects liposuction liquid, and physiological saline injection and discharge state are kept in injection process, makes it internal in flowing
The liposuction liquid of injection is carried out primary wash by state；
Pipeline fill assembly and waste liquid discharge component are closed, allow washing cylinder to be in closed state, then by S4, extraction
Washing cylinder is rocked；
S5, washing separation, by the washing cylinder after extraction with pipeline fill assembly above level, component is discharged in water in waste liquid
The position of flat lower section is placed, and component is discharged in opening conduits fill assembly and waste liquid, allows physiological saline to restore flowing, will carry
The impurity formed in the process is taken to be discharged；
S6, extraction are separately connected syringe A and syringe B in a two fatty mouthful positions, syringe A are extracted physiological saline, then
Pipeline fill assembly and waste liquid discharge component are closed, then the physiological saline in syringe A is re-injected into washing separation
In cylinder, and the adipose tissue finished product present invention after syringe B extraction filter screen filtrations is further arranged to：After S6 extractions,
Second of extraction work can be re-started comprising following steps：
S7, second extraction allow pipeline fill assembly and waste liquid that component is discharged and are closed the end that reopens, and will be obtained in S6
Product is re-injected into washing cylinder, then closes pipeline fill assembly and waste liquid discharge component closure, then
Washing cylinder is rocked；
S8, secondary washing；It will normally be placed by the washing cylinder of S7 processing, pipeline fill assembly allowed to be passed through life after allowing
Brine is managed, then discharge is closed from waste liquid discharge component, the sundries after extraction is discharged;
S9, second decimation use in fatty mouth and operate identical process progress extraction operation with S6.
Through the above technical solutions, the finished product of different size can be obtained by repeatedly extracting, to be suitable for different hands
The Clinical practice of art.
The present invention is further arranged to：During S2, after air discharge, washing cylinder is rocked, then
Washing cylinder is subjected to discharge air further according to the operation of S2.
Through the above technical solutions, by air-discharging again after rocking, it can be by some tiny air for being attached to inner wall
Molecule is discharged, and the solution of internal cavities can be filled.
Description of the drawings
Fig. 1 is the overall schematic of embodiment 1；
Fig. 2 is the explosive view of embodiment 1；
Fig. 3 is the sectional view of embodiment 1；
Fig. 4 is the explosive view of injection head；
Fig. 5 is the enlarged drawing at A in Fig. 4；
Fig. 6 is the sectional view of injection head；
Fig. 7 is flow in pipes connected structure figure；
Fig. 8 is the structure chart of embodiment 2；
Fig. 9 is the enlarged drawing at B in Fig. 8；
Figure 10 is the enlarged drawing at C in Fig. 8.
Reference numeral：1, cylinder is washed；11, extrusion ball；12, link slot；2, pipeline fill assembly；21, flow in pipes；
22, fixed structure；221, buckling groove；222, snap ring；23, connection-peg；231, straight line portion；232, branch line portion；233, puncturing head；
24, switch module；241, movable head；242, catch bar；243, screw rod；244, head is limited；245, face is limited；25, Rubber end；3、
Component is discharged in waste liquid；31, discharge line；32, female Luer；33, waste fluid bag；4, fatty mouth；5, filter screen；51, mounting plate；
52, it is open；53, strainer；54, vertical line；55, x wire；56, isolating bar；561, locating slot；6, plug；61, matrix；62, it holds
Lid；63, installing port；64, substrate；65, connection ring；66, conical opening；67, mounting head；67, interface；68, shim；681, fixed
Position item；7, connection plug；71, capsule is retained；72, intercalation part；73, dosing mouth；74, plug；75, connect band；8, pipe switch；
81, connection sheet；82, installation sheet；83, elastic piece；84, first through hole；85, the second through-hole；861, the first protrusion；862, second is convex
It rises；871, clamping block；872, clamping joint；873, guiding surface；9, pipeline set.
Specific implementation mode
Present invention will now be described in detail with reference to the accompanying drawings..
Embodiment 1
A kind of extraction element, the liposuction liquid for being used to extract are detached, adipose tissue are separated from extract,
The device directly can extract liposuction liquid from human body and carry out separation and Extraction, then directly carry out Clinical practice again.Such as Fig. 1 and
Shown in Fig. 2 comprising the washing cylinder 1 for closing setting, for being stored and being carried the liposuction liquid of extraction
Take separating action.1 one end of washing cylinder is provided with pipeline fill assembly 2, injects physiology salt into washing cylinder 1 for it
Water, the activity of the liposuction liquid for keeping extracting, and washing point is carried out to the liposuction liquid in washing cylinder 1
From.And the other end for washing cylinder 1 is provided with waste liquid discharge component 3, for adipose tissue to be discharged in washing separation process
The waste liquid and impurity of generation.In addition, in order to facilitate extracting and injecting adipose tissue, it is both provided at 1 both ends of washing cylinder
Fatty mouth 4.During extracting separation, liposuction liquid is injected by extraction separation by fatty mouth 4 or fill assembly
In cylinder, and allows pipeline fill assembly 2 and waste liquid that component 3 is discharged simultaneously and work, thus under the flowing of its liquid, by fat
Impurity in fat Extract（Blood plasma, grease etc.）It is taken away, and is floated on the surface since fat stem cell is generally relatively light, in turn
Adipose tissue can remain in cylinder, can complete preliminary separation at this time.
After preliminary separation, as depicted in figs. 1 and 2, and Fig. 3 is combined, be discharged by pipeline fill assembly 2 and waste liquid
Component 3 is closed, and washing 1 interior sealing state of cylinder is kept, and washing cylinder 1 is rocked, allow internal adipose tissue into
Row extrusion and collision to squeeze out the grease in intracellular or tissue, and can allow adipose tissue further to detach refinement,
It allows the diameter of adipose tissue to reduce, makes it more convenient when in use.And in order to make its effect for squeezing when shaking more preferable,
Multiple extrusion balls 11 are provided in washing cylinder 1, extrusion and collision is carried out to adipose tissue by extrusion ball 11, its extruding is allowed to touch
The probability hit increases, and intensity also increases, and to make the effect of the intracellular impurity discharge such as its grease more preferable, general extrusion ball 11 is straight
It connects and is constituted using metal ball, extrusion ball 11 is between 1-10, general setting 6 or so, can be according to the big of washing cylinder 1
It is small to be increased and decreased.
In Clinical practice, the diameter of adipose tissue is limited, and one side is limited by injection position, another party
Face is also limited by injection device.Therefore, as shown in Figure 3 and Figure 4, and with reference to enlarged drawing Fig. 5, fatty mouth 4 is provided with more
It is a, allow the adipose tissue of different size to be extracted out from different fatty mouths 4 by multiple fatty mouths 4, and then allow its can use arrive
Different occasions.And in order to allow different fatty mouths 4 that can extract the adipose tissue of different size out, it is arranged in 4 position of fatty mouth
There is filter screen 5, by filter screen 5 diameter of the adipose tissue of extraction can be applicable in.There are four the settings of fatty mouth 4, similarly
Filter screen 5 includes four, is respectively the filter screen 5 of tetra- kinds of specifications of 2.4mm, 1.2mm, 0.8mm and 0.6mm, respectively one by one
Corresponding each fatty mouth 4, in use, obtain in the adipose tissue of the major diameter specification of 2.4mm, 1.2mm
It is dry thin to can adapt to a variety of fat for the adipose tissue of the adipose tissue and 0.8mm of equal diameter specification or the fine and smooth specification of 0.6mm
The Clinical practice of born of the same parents filled up.Wherein, in order to make adipose tissue more convenient when extracting, by 4 branch of fatty mouth in washing point
From pipe both ends.
Wherein, as shown in Figure 4 and Figure 6, it allows filter screen 5 to be coordinated with fatty mouth 4 for convenience, filter screen 5 is arranged
Into a single integrated structure, i.e., the filter screen 5 at both ends is integral is arranged, and multiple fatty mouths 4 of opposite ends are filtered respectively.Filtering
Net 5 includes mounting plate 51, and the opening 52 to match with fatty mouth 4 is offered on mounting plate 51, strainer is arranged in opening 52
53.Strainer 53 includes that vertical line 54 and x wire 55 intersect to be formed, and it intersects to form square, and strainer 53 and peace
Loading board 51 is directly wholely set.
As shown in fig. 6, plug 6 is respectively set at 1 both ends of washing cylinder, in storage, it can allow it directly by stifled
First 6 are placed, and without rolling, and then are stored also more convenient.It is installed in order to facilitate plug 6, the two of washing cylinder 1
End setting link slot 12, plug 6 is plugged in link slot 12, and is fixed with the inner wall of link slot 12, can be passed through
Fluid sealant is fixed, and can also be fixed by the way of heat seal, so that can be close between plug 6 and washing cylinder 1
Envelope, and the foreign aid of plug 6 and washing cylinder 1 are in the same plane.
As shown in Figure 2 and Figure 7, plug 6 is fixed and is tightly connected with washing cylinder 1.Fatty mouth 4 is separately positioned on stifled
On first 6, and filter screen 5 is installed in plug 6 and one end for connect of washing cylinder 1, filter screen 5 and the use glue of plug 6 into
Row sealing is fixed, or is fixed using heat seal by the way of, and filter screen 5 and washing is allowed to be separated inside cylinder 1.In fat
The end of fat mouth 4 is both provided with conical opening 66, allows fatty mouth 4 that can coordinate with the opening 52 of filter screen 5 by conical opening 66.Together
Sample, it is arranged on plug 6 to allow filter screen 5 to be not in the situation of installation dislocation during installation with reference to Fig. 5
It is useful for separating the shim 68 of each fatty mouth 4, and is provided with positioning strip 681 on shim 68, likewise, filter screen 5
It is provided with the isolating bar 56 for isolating strainer 53, locating slot 561 is provided on isolating bar 56, by locating slot 561 and positioning
Item 681 is matched, to which strainer 53 can be coordinated with corresponding fatty mouth 4.
As shown in fig. 6, plug 6 includes matrix 61 and end cap 62, the mounting head of fatty mouth 4 is provided on matrix 61
67, allow the structure of fatty mouth 4 to be embedded structure, it is whole relatively beautiful to make its surface more smooth.In addition, matrix 61
Including substrate 64 and the connection ring being looped around on substrate 64 65, end cap 62 and 65 heat seal of connection ring, also, by end cap 62 with
Mounting head 67 can be fixed for substrate 64.The installation of head 67 in order to facilitate the installation of, on end cap 62 and on substrate 64
Installing port 63 is opened up, mounting head 67 can be coordinated with installing port 63, and then realizes plugged and fixed.Conical opening 66 is opened in substrate 64
On, allow it to be coordinated with installing port 63, rubber material composition may be used in mounting head 67, with certain elasticity, thus
It is allowed to have the effect of self sealss.During the installation process, connection ring 65 is allowed to be socketed with link slot 12.
As shown in fig. 7, being provided with interface 67 on plug 6, pipeline fill assembly 2 includes flow in pipes 21, interface
67 are arranged on substrate 64, and flow in pipes 21 carries out grafting, 67 L-shape structure setting of interface, interface 67 with interface 67
It extends into filter screen 5 and is connected to setting with 1 inside of washing cylinder across filter screen 5.Interface 67 and flow in pipes 21 it
Between be provided with fixed structure 22, allow with this configuration between flow in pipes 21 and interface 67 realize be detachably connected.In order to allow
Grafting between flow in pipes 21 and interface 67 is more convenient, buckling groove 221 is arranged in 67 position of interface, in flow in pipes
Snap ring 222 is provided on 21, snap ring 222 carries out snapping with buckling groove 221, realizes the connection of flow in pipes 21 and interface 67.
It is fixed with Rubber end 25 in interface 67, is fixed with connection-peg 23 in 21 end of flow in pipes, snap ring 222 is placed in connection-peg
On 23, also, in 23 end set of connection-peg at puncturing head 233, by puncturing head 233 directly through Rubber end 25, its energy is allowed
It enough allows it to be connected to washing cylinder 1 inside with flow in pipes 21, and can allow its inner sealing when extracted, compare
It is convenient.
As shown in fig. 7, wherein connection-peg 23 includes straight line portion 231 and the branch line portion 232 as 23 side of connection-peg, branch
Line portion 232 is wholely set with straight line portion 231, and is all provided with inside straight line portion 231 and branch line portion 232 and is set to hollow structure, and phase
Intercommunicated setting.One end in straight line portion 231 is arranged in puncturing head 233, and 231 other end of straight line portion is provided with switch module
24, it is used for the closing and unlatching of connection-peg 23, branch line portion 232 to be tilted towards 24 one end of switch module.Switch module 24 includes setting
In the catch bar 242 of movable head 241 and the promotion sliding of movable head 241 in straight line portion 231, movable head 241 and straight line portion 231
Inner wall is bonded, and movable head 241 is pushed by catch bar 242, and is blocked between straight line portion 231 and branch line, is reached
The purpose of closing and the unlatching of connection-peg 23.Catch bar 242 is arranged to 243 loop bar of screw rod, catch bar 242 and straight line portion 231
It being rotatablely connected, screw rod 243 is provided on movable head 241, screw rod 243 is threadedly coupled with threaded sleeve rod, when threaded sleeve rod rotates,
Screw rod 243 can be pulled to move axially, and movable head 241 is driven to be moved by screw-thread fit.But in the process used
In, screw rod 243 may be allowed to rotate, and then movable head 241 will not be slided, the effect of switch be not achieved, in order to solve the skill
The limiting structure of the rotation of restricted screw rod 243 is arranged in art problem between straight line portion 231 and screw rod 243.Limiting structure includes setting
It sets the limitation face 245 on screw rod 243 and is slided as the limitation head 244 on straight line portion 231, limitation head 244 and limitation face 245
Dynamic contact, and then achieve the purpose that limiting screw rod 243 rotates.
Waste liquid is discharged component 3 and includes discharge line 31, discharge line 31 and 67 grafting of interface, flow in pipes 21 and discharge
Pipeline 31 is washing 1 both ends of cylinder respectively, and identical as the structure of 67 grafting of interface, and this will not be repeated here.
Embodiment 2
A kind of extraction element, structure are substantially the same with embodiment 1, and difference is flow in pipes 21 and discharge line 31
Connection type, as shown in Figure 8 and Figure 9, in example 2, in order to improve pipeline and wash the leakproofness between cylinder 1, note
Enter pipeline 21 and discharge line 31 is directly fixed on interface 67 by the way of heat seal or gluing, at this point, allowing injection
Pipeline 21 and discharge line 31 directly form integral structure with washing cylinder 1, then allow its sealing effect more preferable.
As shown in Figure 8 and Figure 9, in order to facilitate the keying of pipeline, pipe is provided on flow in pipes 21 and discharge line 31
Road switch 8, flow in pipes 21 and discharge line 31 are constituted using soft liquid-transport pipe-line, by pipe switch 8 can to its into
Row is closed and opens.Pipe switch 8 includes connection sheet 81, installation sheet 82 and elastic piece 83, and first is offered on installation sheet 82
Through-hole 84, flow in pipes 21 or discharge line 31 are directly threaded through the installation that installation sheet 82 is realized in first through hole 84.Connection sheet
81 are wholely set and its link position offers the second through-hole 85 with elastic piece 83, and flow in pipes 21 or discharge line 31 are directly worn
It is located at the installation that connection sheet 81 and elastic piece 83 are realized on the second through-hole 85, elastic piece 83 and 81 setting at an acute angle of connection sheet, and
And connection sheet 81 and 82 integrally connected of installation sheet, i.e. elastic piece 83 and installation sheet 82 carry out one with the both ends of connection sheet 81 respectively
Body is arranged.It is provided with the first protrusion 861 in connection sheet 81, the second protrusion 862 is provided on elastic piece 83, in elastic piece 83
It is provided with clamping structure between installation sheet 82, when elastic piece 83 and the clamping of installation sheet 82, the first protrusion 861 and second is convex
862 mutual extrusion fits are played, to the flow in pipes 21 or discharge line that are each passed through first through hole 84 and the second through-hole 85
31 carry out extruding closure, and the second protrusion 862 is taken away the first protrusion by elastic piece 83 when elastic piece 83 and installation sheet 82 disengage
861, and flow in pipes 21 or discharge line 31 is allowed not to be squeezed and open.Clamping structure includes being arranged in installation sheet 82
On clamping block 871 and be located at the clamping joint 872 of 83 end of elastic piece, clamping joint 872 is towards installation sheet 82 and connection sheet
81 side is provided with guiding surface 873, and clamping joint 872 abuts the purpose for completing clamping with clamping block 871.
In order to allow flow in pipes 21 and discharge line 31 unlatching and closure can effectively implement, in flow in pipes 21
And at least two pipe switch 8 are both provided on discharge line 31, it is both provided with pipeline set 9 in 8 position of each pipe switch,
9 pairs of pipe switch 8 are covered by pipeline to limit, therefore the diameter of pipeline set 9 is more than first through hole 84 and the second through-hole 85
Diameter generally can be respectively provided with pipeline 9 pairs of pipe switch 8 of set in the both sides of each pipe switch 8 and limit.
As shown in figs, flow in pipes 21 needs to connect normal saline bag, is connected in order to facilitate it, in injection pipe
The end set connection plug 7 in road 21, connection plug 7 include intercalation part 72 and retain capsule 71, retain capsule 71 and flow in pipes
21 are wholely set, and intercalation part 72, which is fixed on, to retain on capsule 71, and the end of intercalation part 72 is spine structure, can effectively puncture entrance
In normal saline bag, and hollow structure will be arranged to inside intercalation part 72, physiological saline can be entered along intercalation part 72
To retaining in capsule 71, retaining the setting of capsule 71 can allow a physiological saline part to be retained in inside, and then replace physiology
It, need not air-discharging again when bag of saline.Sometimes in order to add drug, dosing mouth 73 can be set on intercalation part 72, and
Dosing mouth 73 and the inside of intercalation part 72 is allowed to be connected to, and in order to allow entire intercalation part 72 to seal, 73 position of dosing mouth is provided with
Plug 74 made of rubber material are arranged connect band 75 and are attached Anti-fall between plug 74 and intercalation part 72.
As shown in figure 8, discharge line 31 generally requires connection waste fluid bag 33, waste fluid bag 33 generally has a pipeline, in order to
The installation for facilitating waste fluid bag 33 is attached admittedly between the pipeline on waste fluid bag 33 and discharge line 31 by female Luer 32
It is fixed.
Embodiment 3
A kind of operating method of extraction element, includes the following steps：
S1, pipeline grafting；Flow in pipes 21 and discharge line 31 are inserted on washing cylinder 1（Injection pipe in embodiment 1
Road 21 needs to carry out grafting with discharge line 31, and flow in pipes 21 does not need grafting with discharge line 31 in example 2）,
Then flow in pipes 21 is connect into normal saline bag, discharge line 31 connects waste fluid bag 33.
S2, air-discharging；Washing cylinder 1 is inverted, physiological saline is entered into washing cylinder 1 from flow in pipes 21,
The discharge of the air in cylinder 1 will be washed, can be rocked after the completion of injection, some is allowed to be attached to the small sky on barrel
Qi leel is discharged.
S3, primary wash；Washing cylinder 1 is turned over by the injection and discharge for keeping physiological saline from inverted direction
Come, liposuction liquid is injected from fatty mouth 4, carries out washing preliminary filtering in this process.
S4, extraction；Flow in pipes 21 and discharge line 31 are closed, allow 1 inside of washing cylinder to be in closed state, so
Washing cylinder 1 is rocked afterwards.
S5, washing separation；Washing cylinder 1 after extraction normally to be placed, i.e., discharge tube is placed in horizontal down,
Then it allows flow in pipes 21 and discharge line 31 to carry out the flowing of physiological saline, and then some impurity of extraction is gone
It removes.
S6, extraction；The syringe A and syringe B of 10ml are all connected in two different fatty 4 positions of mouth respectively, it will wherein one
The syringe A of position is placed in horizontal down, and extracts a certain amount of（Generally 10ml）Physiological saline, syringe B is failure to actuate；
Then flow in pipes 2121 and discharge line 31 are closed, the physiological saline in syringe A is injected into washing separation
In cylinder 11, syringe B is filled automatically, is obtained the 55 filtered adipose tissue of filter screen of corresponding position, is typically resulted in 1.2mm's
Finished product.
S7, second extraction；It allows flow in pipes 2121 and discharge line 31 to reopen, and the end obtained in S6 is produced
Product（Pass through syringe B）It is re-injected into washing cylinder 1, then closes flow in pipes 21 and discharge line 31,
Washing cylinder 1 is rocked again.
S8, secondary washing；It will normally be placed by the washing cylinder 1 of S7 processing, then allow flow in pipes 21 logical
Enter physiological saline, be then discharged from discharge line 31, the sundries after extraction is discharged.
S9, second decimation；By 4 position of the fatty mouth connection syringe B of first time injection（It does not extract after the implantation）With
And another fatty mouth 4（Its 53 diameter of strainer is less than extracts 53 diameter of strainer for the first time）Position is all connected with the syringe C of 10ml, will
Syringe B extracts a certain amount of（Generally 10ml）Physiological saline, then flow in pipes 21 and discharge line 31 are carried out
It is closed, the physiological saline in syringe B is being injected into washing cylinder 11, syringe C is filled automatically, obtains corresponding position
5 filtered adipose tissue of filter screen, typically result in the finished product of 0.6mm, by second decimation, allow the finished product of 0.6mm
The strainer 53 using 1.2mm after the strainer 53 of the positions 2.4mm is first passed through, finally again by 0.6 strainer 53, to carry out three
Secondary filtering allows the size of its product to be gradually reduced, and prevents its blocking, operation more convenient.
S10, device are handled eventually；After device is washed, it is recycled.
The above is only a preferred embodiment of the present invention, protection scope of the present invention is not limited merely to above-mentioned implementation
Example, all technical solutions belonged under thinking of the present invention all belong to the scope of protection of the present invention.It should be pointed out that for the art
Those of ordinary skill for, several improvements and modifications without departing from the principles of the present invention, these improvements and modifications
It should be regarded as protection scope of the present invention.
Claims (10)
1. a kind of extraction element, which is characterized in that including,
Wash cylinder（1）, washing separation is carried out for storing liposuction liquid, and to the extract；
Plug（6）, it is separately mounted to washing cylinder（1）Both ends, for washing cylinder（1）Closing；
Fatty mouth（4）, several are offered, and be respectively arranged at plug（6）On, with washing cylinder（1）Inside connection；
Filter screen（5）, it is respectively placed in plug（6）With washing cylinder（1）Between, with fatty mouth（4）It is corresponding, for fatty group
The filtering knitted；
The plug（6）On be provided with interface（67）, and wash cylinder（1）The plug at both ends（6）On be connected separately with pipe
Road fill assembly（2）And component is discharged in waste liquid（3）, the pipeline fill assembly（2）Physiological saline is injected into washing cylinder
（1）Liposuction liquid is carried out component is discharged through waste liquid after washing separation（3）Discharge, liposuction liquid washing detach after by
Strainer（5）By fatty mouth after filtering（4）Extraction.
2. extraction element according to claim 1, which is characterized in that the plug（6）Including matrix（61）And installation
In matrix（61）On end cap（62）, described matrix（61）With washing cylinder（1）It is fixedly connected, the end cap（62）It is located at
Matrix（61）On, the fat mouth（4）With interface（67）It may be contained within matrix（61）On, the filter screen（5）Mounted on base
Body（61）On, and filter screen（5）With fatty mouth（4）Corresponding setting.
3. extraction element according to claim 2, which is characterized in that the filter screen（5）Including mounting plate（51）And
It is opened in mounting plate（51）On opening（52）, the opening（52）Inside it is provided with strainer（53）, the strainer（53）With fat
Mouthful（4）It is oppositely arranged.
4. extraction element according to claim 3, which is characterized in that the opening（52）Quantity and fatty mouth（4）Quantity
Equal setting.
5. extraction element according to claim 2, which is characterized in that described matrix（61）On be provided with conical opening（66）,
The conical opening（66）With fatty mouth（4）Connection.
6. extraction element according to claim 1, which is characterized in that the washing cylinder（1）It is provided at both ends with connection
Slot（12）, the plug（6）It is socketed in link slot respectively（12）On, and plug（6）With washing cylinder（1）Sealed set.
7. extraction element according to claim 1, which is characterized in that the washing cylinder（1）Inside it is provided with several squeeze
Pressure ball（11）.
8. a kind of operating method of extraction element as described in claim 1-7 any one, it is characterised in that including walking as follows
Suddenly：
S1, pipeline connection, by flow in pipes（21）Connect normal saline bag, discharge line（31）Connect waste fluid bag（33）；
S2, air-discharging will wash cylinder（1）It is inverted, allows waste liquid that component is discharged（3）It is placed in above level, by normal saline bag
Interior physiological saline is injected into washing cylinder（1）It is interior, cylinder will be washed（1）Interior air discharge；
S3, primary wash will wash cylinder（1）Overturning is just set, and component is discharged in waste liquid（3）It is placed in horizontal down, from it
In a fatty mouth（4）Liposuction liquid is injected, and keeps physiological saline injection and discharge state in injection process, is allowed inside it
In flow regime, the liposuction liquid of injection is subjected to primary wash；
S4, extraction, by pipeline fill assembly（2）And component is discharged in waste liquid（3）It is closed, allows and wash cylinder（1）In closing
Then state will wash cylinder（1）It is rocked；
S5, washing separation, by the washing cylinder after extraction（1）With pipeline fill assembly（2）Above level, waste liquid discharge group
Part（3）It is placed in the position of horizontal down, opening conduits fill assembly（2）And component is discharged in waste liquid（3）, allow physiology salt
Water restores flowing, and the impurity formed in extraction process is discharged；
S6, extraction are separately connected syringe A and syringe B in a two fatty mouthful positions, syringe A are extracted physiological saline, then
By pipeline fill assembly（2）And component is discharged in waste liquid（3）It is closed, then the physiological saline in syringe A is re-injected into and is washed
Wash cylinder（1）It is interior, and syringe B extracts filter screen（5）Filtered adipose tissue finished product.
9. operating method according to claim 8, which is characterized in that after S6 extractions, can re-start and carry for the second time
Take work comprising following steps：
S7, second extraction, allow pipeline fill assembly（2）And component is discharged in waste liquid（3）Closure reopens, and will be obtained in S6
To finished product be re-injected into washing cylinder（1）It is interior, then by pipeline fill assembly（2）And component is discharged in waste liquid（3）
Closure is closed, then will wash cylinder（1）It is rocked；
S8, secondary washing；By the washing cylinder by S7 processing（1）It is normally placed, allows pipeline fill assembly after allowing（2）
It is passed through physiological saline, component then is discharged from waste liquid（3）It is closed discharge, the sundries after extraction is discharged;
S9, second decimation use in fatty mouth and operate identical process progress extraction operation with S6.
10. operating method according to claim 9, which is characterized in that during S2, after air discharge, will wash
Cylinder（1）It is rocked, then will wash cylinder（1）Operation further according to S2 carries out discharge air.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810502244.8A CN108676702B (en) | 2018-05-23 | 2018-05-23 | Extraction device and operation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810502244.8A CN108676702B (en) | 2018-05-23 | 2018-05-23 | Extraction device and operation method thereof |
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| CN108676702A true CN108676702A (en) | 2018-10-19 |
| CN108676702B CN108676702B (en) | 2021-10-15 |
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| CN201810502244.8A Expired - Fee Related CN108676702B (en) | 2018-05-23 | 2018-05-23 | Extraction device and operation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109833650A (en) * | 2019-03-21 | 2019-06-04 | 北京大学第三医院（北京大学第三临床医学院） | A kind of efficient quickly fat purifying plant and its application method |
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| CN106834121A (en) * | 2010-05-20 | 2017-06-13 | 里波热姆斯国际股份公司 | Apparatus and method for preparing tissue, particularly adipose tissue |
| CN207186830U (en) * | 2017-01-22 | 2018-04-06 | 杭州聚立医疗用品有限公司 | Finished product blood bag with filter |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2315958Y (en) * | 1997-06-17 | 1999-04-28 | 王建华 | Recovery and infusion device for patient oneself blood |
| CN1630526A (en) * | 2001-12-07 | 2005-06-22 | 马克罗珀尔生物外科公司 | Systems and methods for treating patients with processed lipoaspirate cells |
| JP2010246446A (en) * | 2009-04-14 | 2010-11-04 | Olympus Corp | Device for treating adipose tissue and apparatus for separating cell |
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