CN111575148B - Device and method for rapidly extracting DNA in batches - Google Patents
Device and method for rapidly extracting DNA in batches Download PDFInfo
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- CN111575148B CN111575148B CN202010346186.1A CN202010346186A CN111575148B CN 111575148 B CN111575148 B CN 111575148B CN 202010346186 A CN202010346186 A CN 202010346186A CN 111575148 B CN111575148 B CN 111575148B
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Abstract
The invention discloses a device and a method for rapidly extracting DNA in batches, wherein the device comprises a base, a grinding tank and a placing frame, wherein the top surface of the base is connected with a heating box through a bracket; the bottom of the grinding tank is communicated with the shunt pipe through the discharging pipe, the bottom of the grinding tank is provided with filtering holes, the top of the shunt pipe is provided with a plurality of groups of shunt holes at equal intervals, and two sides of the shunt pipe are provided with adjusting devices; the top surface of the base is provided with a placing rack, the placing rack comprises a shell and a centrifuge tube, the shell is arranged on the top surface of the base, the top surface of the shell is provided with a through hole matched with the placing barrel, the through hole is positioned right below the flow dividing hole, and a rotating mechanism is arranged in the shell and connected with the placing barrel through the rotating mechanism; the invention can extract in batches rapidly, and can adjust and change the extraction quantity and the extraction efficiency of each time.
Description
Technical Field
The invention belongs to the technical field of DNA extraction, relates to a device and a method for extracting DNA, and particularly relates to a device and a method for rapidly extracting DNA in batches.
Background
DNA extraction is the process of extracting DNA from the tissues of animals and plants.
In the prior art, the device for extracting the DNA has the problem of singleness, namely, only one group of DNA data can be obtained in one extraction operation process, and the rapid batch extraction cannot be realized; in the extraction process, the extract is not uniformly ground during grinding, and the steps of the extraction operation are relatively more, so that the extraction is inconvenient.
Disclosure of Invention
The invention aims to solve the problem of singleness of a device for extracting DNA in the prior art, namely that only one group of DNA data can be obtained in one extraction operation process, and the rapid batch extraction cannot be realized; in addition, during the extraction process, the extraction is not uniform enough, and the extraction operation steps are relatively more, so that the extraction is inconvenient, and a device and an extraction method for rapidly extracting DNA in batches are provided.
The aim of the invention can be achieved by the following technical scheme:
the device for rapidly extracting the DNA in batches comprises a base, a grinding tank and a placing frame, wherein the top surface of the base is connected with a heating box through a bracket, the grinding tank is arranged in the middle of the heating box, a feed inlet is arranged at the top of the grinding tank and is communicated with a grinding cavity, a spherical grinding cavity is arranged in the grinding tank, and a grinding device is arranged on the grinding tank;
the bottom of the grinding tank is communicated with the shunt pipe through the discharging pipe, the bottom of the grinding tank is provided with filtering holes, the top of the shunt pipe is provided with a plurality of groups of shunt holes at equal intervals, and two sides of the shunt pipe are provided with adjusting devices;
be provided with the rack on the top surface of base, the rack includes casing and centrifuging tube, and the casing is installed on the top surface of base, and the through-hole with placing a section of thick bamboo looks adaptation is seted up to the top surface of casing, and the through-hole is located the tapping hole under, and is provided with slewing mechanism in the casing to be connected with placing a section of thick bamboo through slewing mechanism.
Preferably, the grinding device comprises a first motor, a rotating shaft, a grinding ball, a spring and a movable rod, wherein the first motor is arranged on the top surface of the grinding tank, the output end of the first motor is connected with the rotating shaft, the bottom end of the rotating shaft extends to the inside of the grinding cavity and is rotationally connected with the grinding tank, the middle part of the rotating shaft is movably connected with one end of the movable rod, the other end of the movable rod is connected with the grinding ball, the grinding ball rotates along the grinding cavity, and the bottom of the rotating shaft is connected with the middle part of the movable rod through the spring.
Preferably, the adjusting device comprises a pull handle, a piston rod and a piston, the piston is arranged in the two sides of the shunt tube in a sliding mode, the piston rod is arranged on one side of the piston, the other end of the piston rod penetrates through the shunt tube and is in sliding connection with the shunt tube, and the other end of the piston rod is connected with the pull handle.
Preferably, the rotating mechanism comprises a second motor, a driving shaft, a transmission assembly, a fixed plate and a rotating rod, wherein the second motor is installed on the shell, the output end of the second motor is connected with the driving shaft, the driving shaft is rotatably installed in the shell, the driving shaft is connected with the rotating rod through the transmission assembly, the top end of the rotating rod penetrates through the fixed plate and is rotatably connected with the fixed plate, the top end of the rotating rod is connected with the placing cylinder, the fixed plate is installed on the inner wall of the shell, and the placing cylinder is rotatably connected with the top surface of the shell.
Preferably, the transmission assembly comprises a driving bevel gear and a driven bevel gear, a plurality of groups of driving bevel gears are sleeved on the driving shaft at equal intervals, the driving bevel gears are connected with the driven bevel gears in a meshed mode, and the driven bevel gears are connected with the rotating rods.
Preferably, a heating cavity is formed between the inner wall of the heating box and the outer wall of the grinding tank, a hot water inlet pipe is arranged on one side of the heating box, and a hot water outlet pipe is arranged on the other side of the heating box.
Preferably, the inner wall of the placing cylinder is provided with a rubber pad, and the centrifuge tube is positioned in the placing cylinder.
An extraction method of a device for rapidly extracting DNA in batches, the extraction method of the device for extracting DNA comprises the following steps:
s1, placing a plurality of groups of centrifuge tubes into a placement barrel according to the extraction work requirement, and then pulling a pull handle to enable a piston rod to drive a piston to move along a shunt tube, so that the number of shunt holes on the shunt tube is matched with the number of centrifuge tubes on a placement frame;
s2, adding the extract into a grinding cavity in a grinding tank along a feed port, introducing circulating hot water into the heating cavity in a heating box through a hot water inlet pipe and a hot water outlet pipe, heating the grinding tank, then starting a first motor to work to drive a rotating shaft to rotate, driving a movable rod to rotate by the rotating shaft, enabling a grinding ball on the movable rod to grind the extract in the grinding cavity, adding the extract into the grinding cavity, and then mixing and stirring the extract in the grinding cavity through the rotating movable rod;
s3, opening a valve on the discharging pipe, adding the stirred and mixed extracting solution into a centrifuge tube flowing into the placing rack along the shunt pipe through the discharging pipe, then, restarting the second motor to work, driving the driving shaft to rotate through the meshing effect of the driving bevel gear and the driven bevel gear, driving the placing barrel on the rotating rod to rotate, driving the centrifuge tube in the placing barrel to rotate, standing and precipitating after centrifuging the extracting solution, taking out the centrifuge tube, taking supernatant, adding isopropanol to obtain flocculent DNA, and naturally air-drying and then preserving.
Compared with the prior art, the invention has the beneficial effects that: according to the extraction work requirement, a plurality of groups of centrifuge tubes are placed in a placing cylinder, and then a piston rod drives a piston to move along a shunt tube by pulling a pull handle, so that the number of shunt holes on the shunt tube is matched with the number of centrifuge tubes on a placing frame; therefore, centrifuge tubes with different numbers can be placed according to the number requirement of extracting DNA each time, and the number of the flow-through holes on the shunt tubes can be regulated, so that the shunt holes on the shunt tubes are exactly matched with the centrifuge tubes, and the extracting solution passing through the shunt tubes is exactly flowed into the centrifuge tubes, thereby improving the applicability and flexibility of the extracting device;
adding the extract into a grinding cavity in a grinding tank along a feed port, introducing circulating hot water into the heating cavity in a heating box through a hot water inlet pipe and a hot water outlet pipe, heating the grinding tank, then starting a first motor to work, driving a rotating shaft to rotate, driving a movable rod to rotate by the rotating shaft, grinding the extract in the grinding cavity by a grinding ball on the movable rod, adding the extracting solution into the grinding cavity, mixing and stirring the extracting solution in the grinding cavity by a rotating movable rod, uniformly and fully grinding the extract by a grinding device, and mixing the extract and the extracting solution by the movable rod and the grinding ball on the movable rod, so that grinding and mixing are integrated, and the DNA extraction efficiency of the extract is greatly improved;
opening the valve on the discharging pipe, adding the extract after mixing through the discharging pipe along the shunt tubes and flowing into the centrifuging tube on the rack, then, restarting the second motor work, drive the drive shaft and rotate, the drive shaft passes through the meshing effect of initiative bevel gear and driven bevel gear, drive the section of thick bamboo of placing on the bull stick and rotate, thereby drive the centrifuging tube of placing in the section of thick bamboo and rotate, after the extract centrifugation, take out the centrifuging tube after standing and settling, get the supernatant, and acquire flocculent DNA with the isopropanol, preserve after carrying out natural air-drying, through the slewing mechanism who sets up, can rotate the centrifuging to the centrifuging tube of multiunit simultaneously, thereby realized carrying out quick batch extraction to the DNA of extract.
Drawings
The present invention is further described below with reference to the accompanying drawings for the convenience of understanding by those skilled in the art.
Fig. 1 is a schematic perspective view of the whole structure of the present invention.
Fig. 2 is a front view of the present invention.
Fig. 3 is a schematic structural view of the milling pot according to the present invention.
Fig. 4 is a schematic view of a shunt according to the present invention.
Fig. 5 is a schematic structural view of a rack according to the present invention.
Fig. 6 is a front cross-sectional view of a rack in accordance with the present invention.
In the figure: 1. a base; 2. a bracket; 3. a heating box; 4. a grinding tank; 5. a first motor; 6. a feed inlet; 7. a hot water inlet pipe; 8. a pull handle; 9. a piston rod; 10. a shunt; 11. a placing rack; 12. a grinding chamber; 13. a rotating shaft; 14. grinding balls; 15. a spring; 16. a movable rod; 17. a discharge pipe; 18. a piston; 19. a diversion aperture; 20. a hot water outlet pipe; 21. a heating chamber; 22. a second motor; 23. a housing; 24. centrifuging tube; 25. a drive shaft; 26. a drive bevel gear; 27. a driven bevel gear; 28. a fixing plate; 29. a rotating rod; 30. the cartridge is placed.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in connection with the embodiments, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-6, a device for rapidly extracting DNA in batch comprises a base 1, a grinding tank 4 and a placing frame 11, wherein the top surface of the base 1 is connected with a heating box 3 through a bracket 2, the grinding tank 4 is installed in the middle of the heating box 3, a feeding port 6 is arranged at the top of the grinding tank 4 and is communicated with a grinding cavity 12, a spherical grinding cavity 12 is arranged in the grinding tank 4, and a grinding device is arranged on the grinding tank 4;
the bottom of the grinding tank 4 is communicated with the shunt tube 10 through a discharging pipe 17, the bottom of the grinding tank 4 is provided with filtering holes, the top of the shunt tube 10 is provided with a plurality of groups of shunt holes 19 at equal intervals, and two sides of the shunt tube 10 are provided with adjusting devices;
be provided with rack 11 on the top surface of base 1, rack 11 includes casing 23 and centrifuging tube 24, and casing 23 installs on the top surface of base 1, and the through-hole with placing section of thick bamboo 30 looks adaptation is seted up to the top surface of casing 23, and the through-hole is located the tapping hole 19 under, and is provided with rotary mechanism in the casing 23 to be connected with placing section of thick bamboo 30 through rotary mechanism.
The grinding device comprises a first motor 5, a rotating shaft 13, a grinding ball 14, a spring 15 and a movable rod 16, wherein the first motor 5 is arranged on the top surface of a grinding tank 4, the output end of the first motor 5 is connected with the rotating shaft 13, the bottom end of the rotating shaft 13 extends to the inside of the grinding cavity 12 and is rotationally connected with the grinding tank 4, the middle part of the rotating shaft 13 is movably connected with one end of the movable rod 16, the other end of the movable rod 16 is connected with the grinding ball 14, the grinding ball 14 rotates along the grinding cavity 12, the bottom of the rotating shaft 13 is connected with the middle part of the movable rod 16 through the spring 15, extracts are added into the grinding cavity 12 in the grinding tank 4 along a charging hole 6, circulating hot water is introduced into a heating cavity 21 in a heating box 3 through a hot water inlet pipe 7 and a hot water outlet pipe 20, the grinding tank 4 is heated, then, the first motor 5 is started to drive the rotating shaft 13 to rotate, the movable rod 16 is enabled to rotate, the grinding ball 14 on the movable rod 16 grinds extracts in the cavity 12, then extracts in the grinding cavity 12 are added into the grinding cavity 12, then extracts are evenly mixed with the extracts in the grinding cavity 12 through the movable rod 16, and the mixing efficiency of the extracts is improved, and the extracts are fully mixed with the extracts through the movable rod 16, and the grinding device is fully achieved.
The adjusting device comprises a pull handle 8, a piston rod 9 and a piston 18, wherein the piston 18 is arranged in the two sides of the shunt tube 10 in a sliding manner, the piston rod 9 is arranged on one side of the piston 18, the other end of the piston rod 9 penetrates through the shunt tube 10 and is in sliding connection with the shunt tube 10, the other end of the piston rod 9 is connected with the pull handle 8, a plurality of groups of centrifuge tubes 24 are placed into a placement barrel 30 according to the extraction work requirement, and then the piston rod 9 drives the piston 18 to move along the shunt tube 10 by pulling the pull handle 8, so that the number of the shunt holes 19 on the shunt tube 10 is matched with the number of the centrifuge tubes 24 on the placement frame 11; therefore, centrifuge tubes 24 with different numbers can be placed according to the number requirement of DNA extraction at each time, and the number of the flow distribution holes 19 on the shunt tubes 10 can be regulated to enable the flow distribution holes 19 on the shunt tubes 10 to be exactly matched with the centrifuge tubes 24, and the extracting solution passing through the shunt tubes 10 exactly flows into the centrifuge tubes 24, so that the applicability and flexibility of the extracting device are improved.
The rotating mechanism comprises a second motor 22, a driving shaft 25, a transmission assembly, a fixing plate 28 and a rotating rod 29, wherein the second motor 22 is arranged on the shell 23, the output end of the second motor 22 is connected with the driving shaft 25, the driving shaft 25 is rotatably arranged in the shell 23, the driving shaft 25 is connected with the rotating rod 29 through the transmission assembly, the top end of the rotating rod 29 penetrates through the fixing plate 28 and is rotatably connected with the fixing plate 28, the top end of the rotating rod 29 is connected with the placing cylinder 30, the fixing plate 28 is arranged on the inner wall of the shell 23, and the placing cylinder 30 is rotatably connected with the top surface of the shell 23.
The transmission assembly comprises a driving bevel gear 26 and a driven bevel gear 27, a plurality of groups of driving bevel gears 26 are sleeved on the driving shaft 25 at equal intervals, the driving bevel gears 26 are meshed with the driven bevel gears 27, the driven bevel gears 27 are connected with a rotating rod 29, a second motor 22 is started to work to drive the driving shaft 25 to rotate, the driving shaft 25 drives a placing barrel 30 on the rotating rod 29 to rotate through the meshing effect of the driving bevel gears 26 and the driven bevel gears 27, a centrifuge tube 24 in the placing barrel 30 is driven to rotate, after the extracting solution is centrifuged, the centrifuge tube 24 is taken out after standing and precipitating, supernatant is taken out, isopropanol is added to obtain flocculent DNA, the flocculent DNA is stored after natural air drying, and the centrifugal tube 24 of the plurality of groups can be rotated and centrifuged simultaneously through a set rotating mechanism, so that the quick batch extraction of the DNA of the extract is realized.
A heating cavity 21 is formed between the inner wall of the heating box 3 and the outer wall of the grinding tank 4, a hot water inlet pipe 7 is arranged on one side of the heating box 3, a hot water outlet pipe 20 is arranged on the other side of the heating box 3, and the heating cavity 21 is arranged to heat the extract when mixing with the extracting solution, so that mixing of DNA and the extracting solution is facilitated.
The inner wall of the placement barrel 30 is provided with a rubber pad, and the centrifuge tube 24 is located within the placement barrel 30.
An extraction method of a device for rapidly extracting DNA in batches, the extraction method of the device for extracting DNA comprises the following steps:
s1, placing a plurality of groups of centrifuge tubes 24 into a placement barrel 30 according to the extraction work requirement, and then pulling a pull handle 8 to enable a piston rod 9 to drive a piston 18 to move along a shunt tube 10, so that the number of shunt holes 19 on the shunt tube 10 is matched with the number of centrifuge tubes 24 on a placement frame 11; therefore, centrifuge tubes 24 with different numbers can be placed according to the number requirement of DNA extraction at each time, and the number of the flow distribution holes 19 on the shunt tubes 10 can be regulated, so that the flow distribution holes 19 on the shunt tubes 10 are exactly matched with the centrifuge tubes 24, and the extracting solution passing through the shunt tubes 10 is exactly flowed into the centrifuge tubes 24, thereby improving the applicability and flexibility of the extracting device;
s2, adding the extract into a grinding cavity 12 in a grinding tank 4 along a feed port 6, introducing circulating hot water into a heating cavity 21 in a heating box 3 through a hot water inlet pipe 7 and a hot water outlet pipe 20, heating the grinding tank 4, then starting a first motor 5 to work to drive a rotating shaft 13 to rotate, and driving a movable rod 16 to rotate by the rotating shaft 13, so that a grinding ball 14 on the movable rod 16 grinds the extract in the grinding cavity 12, then adding the extract into the grinding cavity 12, then mixing and stirring the extract in the grinding cavity 12 through the rotating movable rod 16, uniformly and fully grinding the extract through a grinding device, and mixing the extract and the extract through the movable rod 16 and the grinding ball 14 on the movable rod 16, thereby realizing the integration of grinding and mixing, and greatly improving the DNA extraction efficiency of the extract;
s3, opening a valve on the discharging pipe 17, adding the stirred and mixed extracting solution into the centrifuge tube 24 flowing into the placing rack 11 along the shunt pipe 10 through the discharging pipe 17, then restarting the second motor 22 to work, driving the driving shaft 25 to rotate, driving the placing barrel 30 on the rotating rod 29 to rotate through the meshing effect of the driving bevel gear 26 and the driven bevel gear 27, driving the centrifuge tube 24 in the placing barrel 30 to rotate, centrifuging the extracting solution, standing and precipitating, taking out the centrifuge tube 24, taking out supernatant, adding isopropanol to obtain flocculent DNA, naturally airing and storing, and simultaneously rotating and centrifuging a plurality of groups of centrifuge tubes 24 through a set rotating mechanism, thereby realizing rapid batch extraction of the extracted DNA.
The working principle of the invention is as follows: according to the extraction work requirement, a plurality of groups of centrifuge tubes 24 are placed into a placement barrel 30, and then a piston rod 9 drives a piston 18 to move along a shunt tube 10 by pulling a pull handle 8, so that the number of shunt holes 19 on the shunt tube 10 is matched with the number of centrifuge tubes 24 on a placement frame 11; therefore, centrifuge tubes 24 with different numbers can be placed according to the number requirement of DNA extraction at each time, and the number of the flow distribution holes 19 on the shunt tubes 10 can be regulated, so that the flow distribution holes 19 on the shunt tubes 10 are exactly matched with the centrifuge tubes 24, and the extracting solution passing through the shunt tubes 10 is exactly flowed into the centrifuge tubes 24, thereby improving the applicability and flexibility of the extracting device;
adding the extract into a grinding cavity 12 in a grinding tank 4 along a feed port 6, introducing circulating hot water into a heating cavity 21 in a heating box 3 through a hot water inlet pipe 7 and a hot water outlet pipe 20, heating the grinding tank 4, then starting a first motor 5 to work to drive a rotating shaft 13 to rotate, and driving a movable rod 16 to rotate by the rotating shaft 13, so that a grinding ball 14 on the movable rod 16 grinds the extract in the grinding cavity 12, then adding the extract into the grinding cavity 12, then mixing and stirring the extract in the grinding cavity 12 through the rotating movable rod 16, uniformly and fully grinding the extract through a set grinding device, and mixing the extract and the extract through the movable rod 16 and the grinding ball 14 on the movable rod 16, thereby realizing the integration of grinding and mixing, and greatly improving the DNA extraction efficiency of the extract;
opening a valve on a discharging pipe 17, adding the stirred and mixed extracting solution into a centrifuge tube 24 flowing into a placing rack 11 along a shunt pipe 10 through the discharging pipe 17, then restarting a second motor 22 to work, driving a driving shaft 25 to rotate, driving the driving shaft 25 to rotate through the meshing effect of a driving bevel gear 26 and a driven bevel gear 27, driving a placing cylinder 30 on a rotating rod 29 to rotate, driving the centrifuge tube 24 in the placing cylinder 30 to rotate, taking out the centrifuge tube 24 after centrifuging the extracting solution, standing and precipitating, taking out supernatant fluid, adding isopropanol to obtain flocculent DNA, naturally airing and preserving, and simultaneously rotating and centrifuging a plurality of groups of centrifuge tubes 24 through a set rotating mechanism, thereby realizing rapid batch extraction of the DNA of the extract.
The preferred embodiments of the invention disclosed above are intended only to assist in the explanation of the invention. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise form disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best understand and utilize the invention. The invention is limited only by the claims and the full scope and equivalents thereof.
Claims (2)
1. A device for rapid batch extraction of DNA, characterized in that: the grinding device comprises a base (1), a grinding tank (4) and a placing frame (11), wherein the top surface of the base (1) is connected with a heating box (3) through a support (2), the grinding tank (4) is installed in the middle of the heating box (3), a feed inlet (6) is formed in the top of the grinding tank (4), the feed inlet (6) is communicated with a grinding cavity (12), a spherical grinding cavity (12) is formed in the grinding tank (4), and a grinding device is arranged on the grinding tank (4);
the bottom of the grinding tank (4) is communicated with the shunt tube (10) through a discharging tube (17), the bottom of the grinding tank (4) is provided with a filtering hole, the top of the shunt tube (10) is provided with a plurality of groups of shunt holes (19) at equal intervals, and two sides of the shunt tube (10) are provided with adjusting devices;
the top surface of the base (1) is provided with a placing rack (11), the placing rack (11) comprises a shell (23) and a centrifuge tube (24), the shell (23) is arranged on the top surface of the base (1), the top surface of the shell (23) is provided with a through hole matched with the placing barrel (30), the through hole is positioned under the diversion hole (19), and a rotating mechanism is arranged in the shell (23) and is connected with the placing barrel (30) through the rotating mechanism;
the grinding device comprises a first motor (5), a rotating shaft (13), a grinding ball (14), a spring (15) and a movable rod (16), wherein the first motor (5) is arranged on the top surface of a grinding tank (4), the output end of the first motor (5) is connected with the rotating shaft (13), the bottom end of the rotating shaft (13) extends to the inside of a grinding cavity (12) and is rotationally connected with the grinding tank (4), the middle part of the rotating shaft (13) is movably connected with one end of the movable rod (16), the other end of the movable rod (16) is connected with the grinding ball (14), the grinding ball (14) rotates along the grinding cavity (12), and the bottom of the rotating shaft (13) is connected with the middle part of the movable rod (16) through the spring (15);
the adjusting device comprises a pull handle (8), a piston rod (9) and a piston (18), wherein the piston (18) is arranged in the two sides of the shunt tube (10) in a sliding manner, the piston rod (9) is arranged on one side of the piston (18), the other end of the piston rod (9) penetrates through the shunt tube (10) and is in sliding connection with the shunt tube (10), and the other end of the piston rod (9) is connected with the pull handle (8);
the rotating mechanism comprises a second motor (22), a driving shaft (25), a transmission assembly, a fixed plate (28) and a rotating rod (29), wherein the second motor (22) is installed on the shell (23), the output end of the second motor (22) is connected with the driving shaft (25), the driving shaft (25) is rotatably installed in the shell (23), the driving shaft (25) is connected with the rotating rod (29) through the transmission assembly, the top end of the rotating rod (29) penetrates through the fixed plate (28) and is rotatably connected with the fixed plate (28), the top end of the rotating rod (29) is connected with the placing cylinder (30), the fixed plate (28) is installed on the inner wall of the shell (23), and the placing cylinder (30) is rotatably connected with the top surface of the shell (23);
the transmission assembly comprises a driving bevel gear (26) and a driven bevel gear (27), a plurality of groups of driving bevel gears (26) are sleeved on the driving shaft (25) at equal intervals, the driving bevel gears (26) are connected with the driven bevel gears (27) in a meshed mode, and the driven bevel gears (27) are connected with the rotating rods (29);
a heating cavity (21) is formed between the inner wall of the heating box (3) and the outer wall of the grinding tank (4), a hot water inlet pipe (7) is arranged on one side of the heating box (3), and a hot water outlet pipe (20) is arranged on the other side of the heating box (3);
the inner wall of the placing barrel (30) is provided with a rubber pad, and the centrifuge tube (24) is positioned in the placing barrel (30).
2. An extraction method of the apparatus for rapid mass extraction of DNA according to claim 1, wherein the extraction method of the apparatus for extraction of DNA comprises the steps of:
s1, according to the extraction work requirement, a plurality of groups of centrifuge tubes (24) are placed into a placement barrel (30), and then a piston rod (9) drives a piston (18) to move along a shunt tube (10) by pulling a pull handle (8), so that shunt holes (19) on the shunt tube (10) are matched with the centrifuge tubes (24) on a placement frame (11);
s2, adding the extract into a grinding cavity (12) in a grinding tank (4) along a feed port (6), introducing circulating hot water into a heating cavity (21) in a heating box (3) through a hot water inlet pipe (7) and a hot water outlet pipe (20), heating the grinding tank (4), then starting a first motor (5) to work, driving a rotating shaft (13) to rotate, driving a movable rod (16) to rotate by the rotating shaft (13), enabling a grinding ball (14) on the movable rod (16) to grind the extract in the grinding cavity (12), adding the extract into the grinding cavity (12), and then mixing and stirring the extract in the grinding cavity (12) through the rotating movable rod (16);
s3, opening a valve on a discharging pipe (17), adding the stirred and mixed extracting solution into a centrifuge tube (24) flowing into a placing rack (11) along a shunt pipe (10) through the discharging pipe (17), then restarting a second motor (22) to work, driving a driving shaft (25) to rotate, driving a placing barrel (30) on a rotating rod (29) to rotate through the meshing effect of a driving bevel gear (26) and a driven bevel gear (27), driving the centrifuge tube (24) in the placing barrel (30) to rotate, taking out the centrifuge tube (24) after centrifuging the extracting solution, standing and precipitating, taking out supernatant, adding isopropanol to obtain flocculent DNA, and naturally air-drying and storing.
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| CN202010346186.1A CN111575148B (en) | 2020-04-27 | 2020-04-27 | Device and method for rapidly extracting DNA in batches |
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| CN202010346186.1A CN111575148B (en) | 2020-04-27 | 2020-04-27 | Device and method for rapidly extracting DNA in batches |
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| CN111575148B true CN111575148B (en) | 2023-05-16 |
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| CN208695218U (en) * | 2018-04-02 | 2019-04-05 | 天津以科美科技发展有限公司 | A kind of chemical reagent grinding device |
| CN208857270U (en) * | 2018-09-07 | 2019-05-14 | 卡替（上海）生物技术股份有限公司 | Stem cell extraction device |
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| US6902703B2 (en) * | 1999-05-03 | 2005-06-07 | Ljl Biosystems, Inc. | Integrated sample-processing system |
| US7695688B2 (en) * | 2003-09-19 | 2010-04-13 | Applied Biosystems, Llc | High density plate filler |
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| CN204953144U (en) * | 2015-09-11 | 2016-01-13 | 上海迈博斯生物医药科技有限公司 | Miniature multipurpose antibody separation and purification device |
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| CN208293007U (en) * | 2018-03-26 | 2018-12-28 | 黄淮学院 | A kind of cotton genomic dna extraction element |
| CN208695218U (en) * | 2018-04-02 | 2019-04-05 | 天津以科美科技发展有限公司 | A kind of chemical reagent grinding device |
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