CN107937258A - A kind of lipochondrion isolates and purifies device and method - Google Patents
A kind of lipochondrion isolates and purifies device and method Download PDFInfo
- Publication number
- CN107937258A CN107937258A CN201711279490.3A CN201711279490A CN107937258A CN 107937258 A CN107937258 A CN 107937258A CN 201711279490 A CN201711279490 A CN 201711279490A CN 107937258 A CN107937258 A CN 107937258A
- Authority
- CN
- China
- Prior art keywords
- lipochondrion
- filter
- rotation axis
- cylinder
- isolates
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
- C12N5/0653—Adipocytes; Adipose tissue
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
Landscapes
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Cell Biology (AREA)
- Rheumatology (AREA)
- Molecular Biology (AREA)
- Sustainable Development (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a kind of lipochondrion to isolate and purify devices and methods therefor, the lipochondrion, which isolates and purifies device, includes lid and cylinder, lid covers forms the depot fat bucket of totally-enclosed on the top of cylinder, lid is equipped with negative port and fatty inhalation interface, inner barrel is provided with least one filter, at least one filter separates the cylinder into multiple separation chambers from top to bottom in cylinder, a rotation axis is provided with the middle part of lid, a separation curet is equipped with rotation axis above each filter, multiple rows of stainless steel filament of longitudinal arrangement is installed in the rotation axis in sample collection room, multiple rows of stainless steel filament is distributed to form separating support around rotation axis.The lipochondrion of the present invention isolates and purifies device, can be under totally-enclosed state, and the oil droplet, Tumescent fluid that fibr tissue, mature cell crush in fatty mixed liquor are dispelled in non-centrifugal full-automatic separation, and according to demand by the lipochondrion hierarchical screening of different thicknesses.
Description
Technical field
The present invention relates to the separation technology field of lipochondrion, and in particular to a kind of lipochondrion isolates and purifies device and side
Method.
Background technology
In fat transplantation operation, through frequently with instrument and equipment it is simpler, cause surgical procedure complicated, tradition
Passed through in technology frequently with non-centrifugation, the fat of suction is poured into gauze and has been placed in basin, by pouring into physiological saline
Fat is rinsed repeatedly to dispel the oil droplet that Tumescent fluid in mixed liquor and mature cell crush, while shreds fiber group using scissors
Knit, processed lipochondrion is then injected into needed by human body filling position by being manually fitted into syringe afterwards.It is this
Operating process complexity is cumbersome, not only the fat of different thicknesses can not be carried out category filter, and cause fat to be exposed to air
In, fat contamination is be easy to cause, causes the risk of human infection, only by the method for normal saline washing, it is difficult to will be ripe fatty
Broken oil droplet is rinsed well, and while extruding filtering moisture by gauze, and the breakage for adding adipocyte is dead, and
Several assistant's cooperations are needed to complete, fat survival is low, and majority is needed to carry out secondary or performed the operation three times to reach expected
Surgical effect.
Traditional suction of fat and the processing procedure to fat, there is following defect:Infection accident easily occurs, causes
Doctor-patient dispute, operates whole exposure in atmosphere, adds the risk of in vitro fat contamination, just in case infect, it will to trouble
Person causes immeasurable actual bodily harm.Great obstacle at heart can be also caused to doctor at the same time, adds the wind of malpractice
Danger and doctor-patient dispute.The fatty process very complicated of processing, it is necessary to which more people coordinate, grow by operating time, is needed in the fat after processing
Streak fibr tissue is picked and is shredded, needs to be stained with to suck with sliver to remove if any entrained oil droplet also bar, oil droplet and Tumescent fluid processing
It is unnet, cause inflammatory reaction, consume substantial amounts of manpower and time, an example fat transfer operation takes 4-5 hour sometimes.
Cannot according to lipochondrion size separation choose requirement, according to since basic unit by different level, lipochondrion from big to small, successively
The operation requirement of filling.Fat passes through multiple conversions, easily causes fat and is damaged, and postoperative fat survival is low, it is necessary to carry out repeatedly
Operation can be only achieved Expected Results, and lipochondrion is uneven, causes fibrous tumour and false cyst.
It can be seen from the above that in the device and technique of traditional fat transfer operation, there is clearly disadvantageous and defect, it would be highly desirable to
Further improve.
The content of the invention
It is an object of the invention to provide a kind of lipochondrion to isolate and purify device and method, to solve existing fat
Separating and purifying technology the degree of automation is low and processing procedure not closed the problem of easily causing infection for grain.
To achieve the above object, the present invention provides lipochondrion and isolates and purifies device, and the lipochondrion isolates and purifies dress
Put including lid and cylinder, the lid, which covers, forms the depot fat bucket of totally-enclosed on the top of cylinder, and the lid is equipped with negative
Mouth and fatty inhalation interface are crimped, the inner barrel is provided with least one filter, and at least one filter is in cylinder
Multiple separation chambers are separated the cylinder into from top to bottom, are sample collection room positioned at the separation chamber of the superiors, positioned at undermost point
It is waste collection room from room, a rotation axis is provided with the middle part of the lid, the rotation axis top is pierced by be formed at the top of lid
Motor connector, the rotation axis lower part extend downwardly in depot fat bucket, in the rotation axis above each filter
A separation curet is equipped with, the separation curet above each filter is parallel with filtering surface thereon to be affixed, the sample receipts
Collect multiple rows of stainless steel filament that longitudinal arrangement is provided with indoor rotation axis, multiple rows of stainless steel filament is around rotation axis distribution shape
Into separating support, the separating support is located above the superiors' filter.
Preferably, the inner barrel is provided with three filters, and three filters are in cylinder from top to bottom by cylinder
Four separation chambers are separated into, are sample collection room positioned at the separation chamber of the superiors, are waste collection positioned at undermost separation chamber
Room.
Preferably, the filter opening aperture of three filters is sequentially reduced from top to bottom.
Preferably, the filter opening aperture of three filters is followed successively by from top to bottom:The filter opening aperture of upper filter is
2.8-3.2mm;The filter opening aperture of middle level filter is 1.8-2.2mm;The filter opening aperture of lower filter is 0.4-0.8mm.
Preferably, positioned at upper strata the sample collection room and be equipped with lipochondrion positioned at middle two separate room and go out
Mouthful, the lipochondrion outlet is positioned close to the position of filtering surface on each filter, and the bottom of the waste collection room
Portion is equipped with Waste outlet.
Preferably, motor is also equipped with the lid, the motor and the motor connector on rotation axis top connect
Connect, drive the rotation axis to rotate.
Preferably, the separation curet scrapes axis to be bar-shaped, described bar-shaped to scrape the parallel interspersed installation of axis on the rotary shaft.
Preferably, adjacent two rows of stainless steel filaments of the separating support distribute alternately.
The present invention also provides a kind of lipochondrion isolation and purification method, the described method includes:By pending adipose tissue
Sample introduces the sample collection of the depot fat Tong Nei the superiors of totally-enclosed by the fatty inhalation interface of sample collection room by negative pressure
Room;Pending adipose tissue sample sprinkle passes through separating support, and the streak tissue in adipose tissue mixed liquor hangs over separation
In multiple rows of stainless steel filament on stent, wherein, the streak tissue includes nerve, blood vessel, fiber and fascia;Turn off negative pressure
Device, pulls out Entering fat silicone tube in fatty inhalation interface and connects physiological saline pipeline, pending using normal saline flushing
Adipose tissue sample;Lipochondrion passes through in cylinder at least one filter being sequentially arranged from top to bottom according to fat through current
Granular size is separated, and is collected respectively;During lipochondrion is rinsed with current, driven and revolved by motor
Shaft constantly rotates, and at least one separation curet set in rotation axis carries out even in the upper filtering surface of each filter respectively
Fast circular-rotation so that lipochondrion is sufficiently separated in current flushing;And the oil droplet in pending adipose tissue sample and
Tumescent fluid and trickle fatty fragment are rinsed to undermost waste collection room and are discharged into waste fluid bag.
Preferably, the motor speed is arranged on less than 300 revs/min.
The invention has the advantages that:
The fat transplantation device and method of the present invention can reduce fatty Infection probability, caused by avoiding fatty bulk aggregation
Fibrocystic and false cyst, effectively dispel oil droplet, reduce inflammatory reaction, and can be broken the fibr tissue selected
Broken processing, is implanted into human body again, and to increase fat stem cell and biological support quantity, the activity of enhancing filling adipocyte, carries
Higher fatty acid survival rate, can meet the requirement of existing fat transfer technology Coleman theory, be provided for fat transfer operation, simpler
Single, convenient, time saving and energy saving method, the present invention by for retain adipocyte activity and axenic purification processing provide safety can
Capable method.
Brief description of the drawings
Fig. 1 is that the lipochondrion of the present invention isolates and purifies the structure diagram of device.
Embodiment
Following embodiments are used to illustrate the present invention, but are not limited to the scope of the present invention.
As shown in Figure 1, a kind of lipochondrion disclosed in the present embodiment, which isolates and purifies device, includes cylinder 1 and lid 2, lid
2 cover the depot fat bucket in the formation totally-enclosed of the top of cylinder 1, and lid 2 is equipped with negative port 21 and fatty inhalation interface 22, cylinder
Body 2 is internally provided with three filters 5,6 and 7, and three filters 5,6 and 7 separate the cylinder into four from top to bottom in cylinder
A separation chamber 11,12,13 and 14, is sample collection room positioned at the separation chamber 11 of the superiors, is positioned at undermost separation chamber 14
Waste collection room, the middle part of lid 2 are provided with a rotation axis 4, and 4 top of rotation axis is pierced by form motor connector from the top of lid 2,
4 lower part of rotation axis extends downwardly in depot fat bucket, is equipped with a separation curet in rotation axis 4 above each filter
9, separation curet 9 scrapes axis to be bar-shaped, and bar-shaped axis of scraping is parallel interspersed in rotation axis 4, and the separation above each filter is scraped
Spoon 9 is parallel with filtering surface thereon to be affixed, and multiple rows of stainless steel that longitudinal arrangement is provided with the rotation axis 4 in sample collection room is thin
Silk 41, multiple rows of stainless steel filament 41 form separating support around the distribution of rotation axis 4, and separating support is located at the superiors' filter 11
Above, adjacent two rows of stainless steel filaments of separating support distribute alternately, positioned at the sample collection room on upper strata and positioned at middle two
Layer separation chamber 12 and 13 is equipped with lipochondrion outlet 111,121 and 131, and lipochondrion outlet 111,121 and 131, which is arranged on, to be leaned on
The position of filtering surface on nearly each filter, and the bottom of waste collection room are equipped with Waste outlet 141.
As described above, sample collection room 11, it is used to collect adipose tissue, and removes nerve, blood vessel, fiber, fascia etc.
Streak tissue;Second layer separation chamber 12, it is connected with sample collection room 11, in sample collection room 11 and second layer separation chamber 12
Between be equipped with upper filter 5;Third layer separation chamber 13, it is connected with second layer separation chamber 12, second layer separation chamber 12 with
Middle level filter 6 is equipped between third layer separation chamber 13;Waste collection room 14, it is connected with third layer separation chamber 13, in waste
Lower filter 8 is equipped between collecting chamber 14 and third layer separation chamber 13, wherein, the filter opening aperture of three filters is from top to bottom
It is sequentially reduced, 8 aperture of filter opening of upper filter 5 is 2.8-3.2mm;8 aperture of filter opening of middle level filter 6 is 1.8-2.2mm;
8 aperture of filter opening of lower filter 7 is 0.4-0.8mm.Adipose tissue sample in sample collection room 11, in sample collection room
In 11, the lipochondrion in adipose tissue sample is by upper filter 5, middle level filter 6 and the filtering point of lower filter 7
After layer, different size of lipochondrion is respectively distributed to sample collection room 11, second layer separation chamber 12 and third layer separation chamber
In 13, lipochondrion of different sizes can be collected respectively as needed.The fat of the present invention isolates and purifies device can be complete
In closed environment, the lipochondrion hierarchical screening of required fibr tissue, different thicknesses is come out.
Specifically, it is a cylinder 1 that fat, which isolates and purifies device, sample collection room 11, second layer separation chamber 12, third layer are divided
Arranged from top to bottom in cylinder 1 from room 13 and waste collection room 14.1 top of cylinder has a lid 2, and lid 2 is equipped with
The negative port 21 and fatty inhalation interface 22 connected with sample collection room 11.There is a rotation axis 4, rotation axis 4 on lid 2
Through sample collection room 11, second layer separation chamber 12 and third layer separation chamber 13.Motor 3 is also equipped with lid 2, it is electronic
Machine 3 is connected with the motor connector on 4 top of rotation axis, drives rotation axis 4 to rotate.In use, negative port 21 is connect first negative
Pressure device, liposuction handle is connected by fatty inhalation interface, and fat is drawn into sample collection room 11 by liposuction handle, when having collected
After adipose tissue sample, fatty inhalation interface is connect into physiological saline input unit, physiological saline is inputted by fatty inhalation interface
Lipochondrion is rinsed, mature cell is effectively dispelled and crushes oil droplet.
Wherein, in rotation axis 4 separation is equipped with upper filter 5, middle level filter 6 and lower filter 7
Curet 9, separated lipochondrion particle diameter is maximum from upper filter 5, and less lipochondrion enters by upper filter 5
Second layer separation chamber 12, then small lipochondrion enter third layer separation chamber 13 through middle level filter 6 again, to lipochondrion point
From during, rotation axis 4 constantly rotates, and the separation curet in rotation axis 4 constantly stirs lipochondrion so that fat
Grain sufficiently carries out separation layering according to size by filter.
Rotation axis 4 in sample collection room 11 is equipped with multiple rows of stainless steel filament 41, enters sample in adipose tissue sample
After collecting chamber 11, multiple rows of stainless steel filament 41 in rotation axis 4 is by the nerve in adipose tissue sample, blood vessel, fiber, fascia etc.
Streak tissue removes.
Be equipped with sample collection room 11, second layer separation chamber 12 and third layer separation chamber 13 lipochondrion outlet 111,
121st, 131, Waste outlet 141 is equipped with 14 bottom of waste collection room, specifically, close to upper filter in sample collection room 11
5 edge sides, second layer separation chamber 12 is close to 6 edge side of middle level filter, and third layer separation chamber 13 is close to 7 edge of lower filter
Side sets lipochondrion to export, and different size of lipochondrion is exported by lipochondrion and exported, the installation of lipochondrion exit
There is rotary switch, control the output of lipochondrion.
Lipochondrion isolation and purification method comprises the following steps disclosed in the present embodiment:
Pending adipose tissue sample is introduced into sample by the fatty inhalation interface of sample collection room 11 by negative pressure
The silicone tube of autoclave sterilization, first, is connected by collecting chamber 11 in negative port 21 with negative pressure device, then will be with high temperature height
Press the liposuction handle of sterilizing, fat suction needle to be connected to sterilized silicone tube at fatty inhalation interface, open negative pressure device, continue
Liposuction is usually no more than 1200ml, adipose tissue suction operation is completed to required fatty dosage;
Turn off negative pressure device, pull out the silicone tube of fatty inhalation interface, be connected with the physiological saline got ready, inhaled in fat
Incoming interface connects physiological saline pipeline, opens lower end waste water mouth switch, opens the switch on upper end water inlet physiological saline pipeline, allows
Physiological saline enters in cylinder, utilizes the adipose tissue sample that normal saline flushing is pending;
Adipose tissue in sample collection room 11 forms lipochondrion, and motor is installed in rotation axis 4, wherein, electricity
The rotating speed of motivation is that during rotation axis 4 is rotating, physiological saline constantly rinses lipochondrion less than 300R/min,
The Waste outlet of waste collection room 14 to be opened, waste liquid continual can be flowed out from Waste outlet, meanwhile, separation curet 9 exists
The upper filtering surface of each filter carries out steady circular's rotation, and constantly different size of lipochondrion is stirred, is made
Lipochondrion is obtained to be sufficiently separated in current flushing.
After the flushing of physiology salt current, different size of lipochondrion filters lipochondrion by upper filter 5, middle level
Device 6 and lower filter 7 are separated lipochondrion according to size, and then different size of lipochondrion is collected,
Oil droplet and Tumescent fluid and trickle fatty fragment in pending adipose tissue sample are rinsed to undermost waste collection room
And it is discharged into waste fluid bag.Observe orlop waste collection room 14, when in waste collection room 14 residual water naked eyes see it is very limpid,
When the oil bloom of floating are also seldom, to turn off physiological saline water inlet switch, stop water inlet, motor continues to keep rotating several seconds, with
The moisture content in fat deposit is reduced, motor is then turned off, exhausts the waste liquid in orlop waste collection room 14.
At this moment in depot fat bucket the fat for the variable grain that each layer is separated be we it is required filling different levels
Finished product, bulky grain play a supportive role, fine particle, which adds, to be filled in filling part needed for the subcutaneous grade of muscle body of gland as basic unit's filler
Position, is more conducive to vascularization, ensures the success of fat transfer operation.Fibr tissue on the superiors' separating support is because containing more
Regeneration of stem cells component, after the lipochondrion of lower floor is extracted out, we can carry out comminuted processing, by upper strata ribbon fibre
Rear Collection utilization is smashed with big lipochondrion.The fat isolated and purified can be gone out with lipochondrion of the syringe in each level
Extract and collect at mouthful, also can be by being injected with the supporting automation of the grease filling system of this coordinative composition of equipments.
The lipochondrion of the present invention isolates and purifies device, can be under totally-enclosed state, and non-centrifugal full-automatic separation is dispelled
Except oil droplet, the Tumescent fluid that fibr tissue, mature cell crush in fatty mixed liquor, and according to demand by the fat of different thicknesses
Grain hierarchical screening.The time of fat transfer operation is shortened, reduces the link of fat transfer operation, avoids fat transfer
Cheng Zhong, fat expose the pollution easily caused in atmosphere, cause the risk of postoperative infection, add the survival rate of fat transfer.
By different level fat is chosen according to variable grain size.According to operation different levels can be required to note when injecting in vivo
Required Grainy fat tissue is penetrated, the fat so transplanted is more conducive to survive and greasing dilatation.Under air-tight state, do not pass through centrifugation
The oil droplet crushed in fat is dispelled, is a difficult problem in fat transfer operation.The present invention completely solves this problem.Liposuction
Operation consent squeezes into internal Tumescent fluid because containing some drug ingedients, and aseptic inflammation reaction is easily caused in transfer operation, non-
The standing method of centrifugation is, it is necessary to which the time of repose of long period, so collecting large capacity fat needs time-consuming very long, delay
Operating time.Being verified by expert clinical in recent years, many authoritative experts propose, the fat transfer such as chest enlarge of large capacity,
Buttocks development surgery is performed the operation, and when choosing purification process fat, farthest to be reduced and be avoided fat impaired, so just can guarantee that transplanting
The survival rate of operation and the success of transfer operation.Processing of the present invention in each link is provided to reduce damage fat, protects
Demonstrate,prove adipocyte activity.Regeneration effect of the bacillar structure in transplanting, is the great discovery in transfer operation in recent years, but point
It is difficult from processing bacillar structure, the mixture containing fibr tissue and fat can only be poured on a vessel by existing doctor
In, the drawbacks of being shredded with scissors, being handled using this method, is very much, infection caused by exposure in atmosphere, and scissors processing is endless
Complete easily cause blocks greasing pipe when injecting fat, the damage caused by adipose tissue in processing procedure, and have impact on fat
Survival rate.
The fat transplantation device and method of the present invention can reduce fatty Infection probability, caused by avoiding fatty bulk aggregation
Fibrocystic and false cyst, effectively dispel oil droplet, reduce inflammatory reaction, and can be broken the fibr tissue selected
Broken processing, is implanted into human body again, and to increase fat stem cell and biological support quantity, the activity of enhancing filling adipocyte, carries
Higher fatty acid survival rate, can meet the requirement of existing fat transfer technology Coleman theory, be provided for fat transfer operation, simpler
Single, convenient, time saving and energy saving method, the present invention by for retain adipocyte activity and axenic purification processing provide safety can
Capable method.
Although above with general explanation and specific embodiment, the present invention is described in detail, at this
On the basis of invention, it can be made some modifications or improvements, this will be apparent to those skilled in the art.Therefore,
These modifications or improvements without departing from theon the basis of the spirit of the present invention, belong to the scope of protection of present invention.
Claims (10)
1. a kind of lipochondrion isolates and purifies device, it is characterised in that the lipochondrion isolate and purify device include lid and
Cylinder, the lid covers forms the depot fat bucket of totally-enclosed on the top of cylinder, and the lid is equipped with negative port and fat
Inhalation interface, the inner barrel are provided with least one filter, and at least one filter is in cylinder from top to bottom by cylinder
Body is separated into multiple separation chambers, is sample collection room positioned at the separation chamber of the superiors, is received positioned at undermost separation chamber for waste
Collect room, a rotation axis is provided with the middle part of the lid, the rotation axis top is pierced by form motor connector at the top of lid, institute
State rotation axis lower part to extend downwardly in depot fat bucket, a separation is equipped with above each filter in the rotation axis
Curet, separation curet above each filter is parallel with filtering surface thereon to be affixed, the rotation axis in the sample collection room
On multiple rows of stainless steel filament of longitudinal arrangement is installed, multiple rows of stainless steel filament is distributed to form separating support around rotation axis, institute
Separating support is stated to be located above the superiors' filter.
2. a kind of lipochondrion according to claim 1 isolates and purifies device, it is characterised in that the inner barrel is set
There are three filters, three filters separate the cylinder into four separation chambers from top to bottom in cylinder, positioned at point of the superiors
It is sample collection room from room, is waste collection room positioned at undermost separation chamber.
3. a kind of lipochondrion according to claim 2 isolates and purifies device, it is characterised in that three filters
Filter opening aperture is sequentially reduced from top to bottom.
4. a kind of lipochondrion according to claim 3 isolates and purifies device, it is characterised in that three filters
Filter opening aperture is followed successively by from top to bottom:
The filter opening aperture of upper filter is 2.8-3.2mm;
The filter opening aperture of middle level filter is 1.8-2.2mm;
The filter opening aperture of lower filter is 0.4-0.8mm.
5. a kind of lipochondrion according to right wants 2 isolates and purifies device, it is characterised in that the sample positioned at upper strata
Collecting chamber and lipochondrion outlet is equipped with positioned at middle two separate room, the lipochondrion outlet is positioned close to each
The position of filtering surface on filter, and the bottom of the waste collection room are equipped with Waste outlet.
6. a kind of lipochondrion according to claim 1 isolates and purifies device, it is characterised in that is also installed on the lid
There is motor, the motor is connected with the motor connector on rotation axis top, drives the rotation axis to rotate.
7. a kind of lipochondrion according to right wants 1 isolates and purifies device, it is characterised in that the separation curet is bar-shaped
Scrape axis, it is described bar-shaped to scrape the parallel interspersed installation of axis on the rotary shaft.
8. a kind of lipochondrion according to right wants 1 isolates and purifies device, it is characterised in that the separating support it is adjacent
Two rows of stainless steel filaments distribute alternately.
9. any a kind of lipochondrion in 1-8 is wanted to isolate and purify the lipochondrion side of isolating and purifying of device according to right
Method, it is characterised in that the described method includes:
Pending adipose tissue sample is introduced to the storage of totally-enclosed by the fatty inhalation interface of sample collection room by negative pressure
The sample collection room of the Zhi Tongnei the superiors;
Pending adipose tissue sample sprinkle passes through separating support, and the streak tissue in adipose tissue mixed liquor hangs over separation
In multiple rows of stainless steel filament on stent, wherein, the streak tissue includes nerve, blood vessel, fiber and fascia;
Turn off negative pressure device, pull out Entering fat silicone tube in fatty inhalation interface and connect physiological saline pipeline, utilize physiological saline
Rinse pending adipose tissue sample;
Lipochondrion passes through in cylinder at least one filter being sequentially arranged from top to bottom according to lipochondrion size through current
Separated, and be collected respectively;
During lipochondrion is rinsed with current, drive rotation axis constantly to rotate by motor, set in rotation axis
At least one separation curet carries out steady circular's rotation in the upper filtering surface of each filter respectively so that lipochondrion is in water
Stream is sufficiently separated in rinsing;And
Oil droplet and Tumescent fluid and trickle fatty fragment in pending adipose tissue sample are rinsed to undermost waste and received
Collection room is simultaneously discharged into waste fluid bag.
10. lipochondrion isolation and purification method according to claim 9, it is characterised in that the motor speed is set
Below 300 revs/min.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711279490.3A CN107937258A (en) | 2017-12-06 | 2017-12-06 | A kind of lipochondrion isolates and purifies device and method |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711279490.3A CN107937258A (en) | 2017-12-06 | 2017-12-06 | A kind of lipochondrion isolates and purifies device and method |
Publications (1)
Publication Number | Publication Date |
---|---|
CN107937258A true CN107937258A (en) | 2018-04-20 |
Family
ID=61944988
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711279490.3A Pending CN107937258A (en) | 2017-12-06 | 2017-12-06 | A kind of lipochondrion isolates and purifies device and method |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN107937258A (en) |
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110317720A (en) * | 2018-11-04 | 2019-10-11 | 郭甜甜 | A kind of experiment of pancreas islet is separated by filtration pipe |
CN110982784A (en) * | 2019-05-13 | 2020-04-10 | 杭州美天美医疗美容门诊部有限公司 | Production method and application of nano fat particles |
CN111068380A (en) * | 2020-01-02 | 2020-04-28 | 刘学军 | Fat autosegregation filter |
CN111135918A (en) * | 2020-01-17 | 2020-05-12 | 中南大学湘雅医院 | Fat cutting device and RAE fat preparation system |
CN112774306A (en) * | 2020-12-17 | 2021-05-11 | 杭州百桥医疗技术有限公司 | Full-automatic separation, preparation and extraction device and method for multiple components of autologous fat |
CN113710293A (en) * | 2019-03-11 | 2021-11-26 | 罗基医疗保健公司 | Method for producing composition for cartilage regeneration using freeze-dried hyaline cartilage powder, composition for cartilage regeneration produced by the method, method for producing patient-customized scaffold for cartilage regeneration using composition for cartilage regeneration, and patient-customized scaffold for cartilage regeneration |
CN114796606A (en) * | 2021-01-20 | 2022-07-29 | 南方医科大学南方医院 | Rigid fat transplantation composite material and preparation method and application thereof |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1723857A (en) * | 2004-07-21 | 2006-01-25 | 上海第二医科大学附属第九人民医院 | Autologous fat transplantation device and lipochondrion screening technique |
JP2009038998A (en) * | 2007-08-07 | 2009-02-26 | Olympus Corp | Cell-separating device |
CN103706556A (en) * | 2013-12-02 | 2014-04-09 | 无锡合众信息科技有限公司 | Material filter |
CN106353492A (en) * | 2016-10-13 | 2017-01-25 | 东南大学 | Cassette device for screening tumor cell aptamers |
CN206562361U (en) * | 2017-02-23 | 2017-10-17 | 云南中贸环境节能科技投资股份有限公司 | A kind of environment-protection wastewater processing equipment |
CN206607231U (en) * | 2017-03-24 | 2017-11-03 | 青岛瑞思德生物科技有限公司 | A kind of fat stem cell filter |
CN207659440U (en) * | 2017-12-06 | 2018-07-27 | 山西阳光中天医疗器械有限公司 | A kind of lipochondrion isolates and purifies device |
-
2017
- 2017-12-06 CN CN201711279490.3A patent/CN107937258A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1723857A (en) * | 2004-07-21 | 2006-01-25 | 上海第二医科大学附属第九人民医院 | Autologous fat transplantation device and lipochondrion screening technique |
JP2009038998A (en) * | 2007-08-07 | 2009-02-26 | Olympus Corp | Cell-separating device |
CN103706556A (en) * | 2013-12-02 | 2014-04-09 | 无锡合众信息科技有限公司 | Material filter |
CN106353492A (en) * | 2016-10-13 | 2017-01-25 | 东南大学 | Cassette device for screening tumor cell aptamers |
CN206562361U (en) * | 2017-02-23 | 2017-10-17 | 云南中贸环境节能科技投资股份有限公司 | A kind of environment-protection wastewater processing equipment |
CN206607231U (en) * | 2017-03-24 | 2017-11-03 | 青岛瑞思德生物科技有限公司 | A kind of fat stem cell filter |
CN207659440U (en) * | 2017-12-06 | 2018-07-27 | 山西阳光中天医疗器械有限公司 | A kind of lipochondrion isolates and purifies device |
Cited By (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN110317720A (en) * | 2018-11-04 | 2019-10-11 | 郭甜甜 | A kind of experiment of pancreas islet is separated by filtration pipe |
CN113710293A (en) * | 2019-03-11 | 2021-11-26 | 罗基医疗保健公司 | Method for producing composition for cartilage regeneration using freeze-dried hyaline cartilage powder, composition for cartilage regeneration produced by the method, method for producing patient-customized scaffold for cartilage regeneration using composition for cartilage regeneration, and patient-customized scaffold for cartilage regeneration |
CN110982784A (en) * | 2019-05-13 | 2020-04-10 | 杭州美天美医疗美容门诊部有限公司 | Production method and application of nano fat particles |
CN110982784B (en) * | 2019-05-13 | 2023-12-29 | 杭州美天美医疗美容门诊部有限公司 | Production method and application of nano fat particles |
CN111068380A (en) * | 2020-01-02 | 2020-04-28 | 刘学军 | Fat autosegregation filter |
CN111135918A (en) * | 2020-01-17 | 2020-05-12 | 中南大学湘雅医院 | Fat cutting device and RAE fat preparation system |
CN112774306A (en) * | 2020-12-17 | 2021-05-11 | 杭州百桥医疗技术有限公司 | Full-automatic separation, preparation and extraction device and method for multiple components of autologous fat |
CN114796606A (en) * | 2021-01-20 | 2022-07-29 | 南方医科大学南方医院 | Rigid fat transplantation composite material and preparation method and application thereof |
CN114796606B (en) * | 2021-01-20 | 2024-03-01 | 南方医科大学南方医院 | Rigid fat transplantation composite material and preparation method and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN107937258A (en) | A kind of lipochondrion isolates and purifies device and method | |
CN207659440U (en) | A kind of lipochondrion isolates and purifies device | |
US11389616B2 (en) | Method for preparing tissue, particularly adipose tissue, for transplantation from lobular fat extracted by liposuction | |
CN103330961B (en) | A kind of medical cavity content target component reclaims apparatus | |
CN204874518U (en) | Intestinal content target composition draws equipment | |
CN208145509U (en) | A kind of crushing extraction integrated apparatus | |
US11028360B2 (en) | Separator for microorganisms in cavitary contents | |
CN108014384A (en) | A kind of closed full-automatic fat transfer equipment and its fat transfer method | |
CN104363972A (en) | Centrifugal dynamic filtering apparatus and cell separation system using same | |
CN108315297A (en) | A method of it detached from adipose tissue, purify fat stem cell | |
CN109700488A (en) | A kind of external fine grained fat prepares all-in-one machine | |
CN103484365B (en) | A kind of devices and methods therefor obtaining stem cell from tissue | |
CN101693884A (en) | Method for separating and extracting stem cells from placenta, umbilical cord or adipose tissue | |
CN107338219A (en) | A kind of separation of placenta mesenchyma stem cell and cultural method | |
CN108486057B (en) | Method for extracting hematopoietic stem cells from placenta by using multifunctional placenta tissue processing device | |
CN206896543U (en) | Full-automatic autologous fat breast-enlarging apparatus | |
CN208414445U (en) | A kind of adipose tissue separator | |
CN209081887U (en) | A kind of enteric microorganism separator | |
KR101316574B1 (en) | Multiway valve and System for extracting regenerative cells using the same | |
CN206654917U (en) | SVF automatic cell separating devices | |
AU2012229889A1 (en) | Cell processing method and device | |
CN207452106U (en) | For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction | |
CN107058084A (en) | SVF automatic cell separating devices and its method | |
CN209564684U (en) | A kind of autologous fat separating and treating apparatus | |
CN209092303U (en) | A kind of fatty purification device of liposuction filling |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |