CN207452106U - For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction - Google Patents

Abstract

The utility model patent of the utility model is a kind of fat-derived primary stem cell collection piece-rate system of binary channels for being used for human body liposuction, is mainly formed and is linked in sequence by 24 component structurals and its fixing device.It can be not only used for fat human body liposuction and adipose tissue acquisition, it can be extracted again to collecting the cellular component in adipose tissue with separating, fat tissue cell can also be separated again simultaneously, and fatty granule cell, stromal vascular fraction and its fat-derived seed cell of the primary stem cell of fat source and passage that the autotransplantation for skin anti-aging and face rejuvenation can be obtained with this etc..The utility model patent is upper safer in application compared with similar product, easier in operation, and using upper more flexible, liposuction is more efficient, and operation is more time saving, and saves self primary seed cell resource, and energy conservation and environmental protection reduces environmental pollution, and is easy to popularize.

Description

For the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction

Technical field

The utility model patent belongs to a kind of special equipment for being useful in human body beauty treatment and the application of body beautification field, and in particular to One kind can be used for the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction.

Background technology

It is very popular in the present day plastic surgical operation and the technical method of minimally invasive medicine body beautification and beauty used at present The adipose tissue that self fat position is excessively enriched with directly is extracted out, fat body part superabundant fats are quickly subtracted so as to reach It organizes and realizes moulding and body beautification purpose.Meanwhile also there is cosmetic plastic surgery doctor that these are had been pumped out what is come again at present Extra autologous adipose tissue and from these adipose tissues by distinct methods extraction with separated fatty granule cell and its Linked groups' cell is transplanted to body again（Including face, neck, chest, buttocks etc.）Required progress beauty and body beautification and skin The anti-aging position with face rejuvenation, and go to replace with the autologous tissues such as this adipose tissue or fatty granule cell or cellular component For those heterologous chemical substances or xenogenesis or allosome bion soft tissue filler, and it is desirable that solved and overcome some different with this Source property chemical type or bion soft tissue filler transplanting after easily occur allergic immune reaction or allergic stimulated reaction and progressively The deficiencies of degradation or absorption.

Using self superabundant fats tissue and adipocyte that these are extracted from body fat position, this can be not only avoided The unnecessary waste or discarded of a little valuable human fatty tissue and adipocyte resources, moreover, can also utilize these preciousnesses Autologous adipose tissue, and therefrom extract and isolate a variety of periphery adipose tissue competent cells with high bioactivity（Bag Include adipose stromal cells, vascular endothelial cell, fibroblast, macrophage, Preadipocyte, fat-derived stem cells and Fat mesenchymal stem cell）, moreover, it is also possible to therefrom extract and isolate a large amount of extracellular matrix components, cell factor and life The living resources of the preciousness such as long factor.And it lives from the autogenous cells and its natural biological for wherein separating these a large amount of preciousnesses obtained Property substance, can be fed back again using minimally invasive beautifying technique or implantation technique is carried out Soft-tissue operation again, for improve or Those body surface defects and Facial Depression or defect etc. are modified, can especially be migrated to facial skin aging position, so as to Delay or mitigate skin natural aging and light aging, improve that chest, bung fat tissue development be bad and other body parts Adipose tissue, which is unevenly distributed and extends, maintains transplant fat tissue and Grainy fat tissue cells survival time and stability and persistently Property etc. have important value.

Particularly, can also have been extracted out in vitro from these by the stem cell isolation techniques such as Density Gradient Centrifugation means and method Superabundant fats tissue and adipocyte in largely extract and separate and obtain the fat-derived primary stem cell of more precious people （) or fat mesenchymal stem cell and its cytokine profiles and growth factor and fat of their institute's endocrines and paracrine ADSCs The fat factor, such as vascular endothelial growth factor (VEGF), para-insulin like growth factor (IGF), hepatocyte growth factor (HGF), transforming growth factor (TGF-β), basic fibroblast growth factor (bFGF), acid fibroblast growth factor (aFGF), platelet derived growth factor (PDGF), epithelical cell growth factor (EGF), interleukins (IL), interferon (INF), Tumor necrosis factor-alpha (TNF-α), leptin, Adipocyte Factor and Adipose hormone etc..Especially, it is drawn to from these external more The fat-derived primary stem cell extracted and separated and obtain in remaining adipose tissue and adipocyte（ADSCs) or between fat fill Matter stem cell not only can directly carry out the cell quantity and quality that transplanting improves in situ tissue, but also it is fat-derived to transplant these Primary stem cell（ADSCs) or fat mesenchymal stem cell can also improve the quality and quantity of transplantation site cell and cell is lived Power, and ADSCs itself still can carry out the multipotency of orderly self-replacation, self duplication, self-regeneration and self-regeneration into Somatic stem cell.These fat-derived stem cells or fat mesenchymal stem cell are scientifically and rationally transplanted to the portion that needed by human body wants Position, they can induce and be divided into vivo body or the required various cambiums of tissue and neonatal cell.Moreover, this Various cell factors and growth factor secreted by a little fat-derived stem cells or fat mesenchymal stem cell, can not only accelerate to promote Into the synthesis and secretion with stimulation dermal tissue collagen, moreover it is possible to quickly promote the migration of skin corium fibroblast (HDF) With aggregation, division with multiplication, differentiation with conversion etc. cell biocompatibilities, so as to, can effectively delaying skin it is physiological aging, anti- Control skin photoage and ultraviolet injury etc..In addition, fat-derived stem cells（) or fat-derived mescenchymal stem cell institute ADSCs All multiple cytokines of secretion and growth factor etc. can also each stimulate the multiplication of corresponding target cell and cellular matrix to synthesize and divide It secretes, so as to the skin histology and cell for effectively delaying or improving skin histology aging, repair Aging Damage, so as to improve aging Skin texture and physiological function.Clinic also has application attestation, is extracted in injection in skin histology from autologous adipose tissue Stromal vascular fraction and its cell（Containing the interior fat-derived stem cells there are about 20-30%）, dermis thickness can be made substantially to increase, Aging wrinkle significantly reduces.

For this purpose, human body liposuction method and the research and development of instrument to being applied in clinical medicine beauty and plastic surgery beauty by To both at home and abroad with subject and with specialty and medical cosmetology field more and more extensive concern, attention and input.Relevant technical people Various technical methods and instrument and equipment constantly invention and improvement of the member to these extraction autologous adipose tissues, it is desirable to can be prepared with this With obtain more, quality higher, the particularly better autogenous cell of application effect, fat stem cell.Current clinically institute Most of the human body liposuction technology of application and its method are to rely on or rely on some instrument and equipments to carry out or first using operation A skin osculum is cut, some medicaments and instrument is then recycled to stretch into subcutaneous tissue fat deposit by this skin incision Superabundant fats tissue is suctioned out to external method.And with the present invention is closely related can be used for extracting the extra fat of subcutis The technology of fat is summed up mainly has following three kinds of methods at present：When lipsuction after ultrasonic wave effect, second is that electromagnetic effect Lipsuction afterwards, third, quickly forming Negative-pressure Liposuction.Lipsuction is similar to the work of ultrasonic cell disintegration instrument after ultrasonic wave effect Principle is typically generated the ultrasonic wave of specific frequency using ultrasonic generator, and passes through the ultrasonic by human body of this specific frequency Subcutaneus adipose tissue or adipocyte or lipochondrion crush, and the mode then progressively absorbed by subcutaneous tissue reaches noninvasive suction The purpose of fat weight-reducing body beautification or the metal straw body for connecting vacuum and negative pressure pump is positioned over subcutaneously by small and concealed incision again, it is logical It crosses and to form quick negative pressure and suction out the adipose tissue crushed or adipocyte or lipochondrion, to reach liposuction weight-reducing body beautification Purpose.The principle and method of lipsuction and lipsuction after ultrasonic wave effect are substantially similar after electromagnetic effect, simply electromagnetic wave effect Lipsuction is that cellulite of the high-frequency electric field to subcutaneous tissue of certain specific wavelength is generated by the two poles of the earth of electromagnetic generator after answering Block carries out destruction, it is made, which to generate galvanomagnetic-effect to fat, makes adipose tissue formation chyle sample liquid, then by subcutaneous The mode progressively absorbed is organized to achieve the purpose that noninvasive liposuction weight-reducing body beautification or born again by small and concealed incision by vacuum is connected The metal straw body of press pump is positioned over subcutaneously, by forming quick negative pressure by the adipose tissue crushed or adipocyte or fat Grain suctions out, to achieve the purpose that liposuction weight-reducing body beautification.And quickly form Negative-pressure Liposuction, then it is by subcutis The big volume low-concentration of superabundant fats injection location contains adrenergic lidocaine brine, is allowed to that swelling occurs until there is skin group Hair-weaving is white, so that penetrating into a large amount of brine in adipocyte or lipochondrion and voluntarily rupturing dissolving, then passes through small and concealed incision The metal straw body for connecting vacuum and negative pressure pump is positioned over subcutaneously, by forming quick negative pressure by adipose tissue or adipocyte or fat Fat particle suctions out, to achieve the purpose that liposuction weight-reducing body beautification.

In relation to relative deficiency, defect and problem existing for human body liposuction correlation technique and method in the background technique：

The closely related background technology with the present invention, i.e. three kinds of skills currently used for extracting subcutis superabundant fats Art method has a reapective features and advantage, but the practical application also some deficiencies of objective reality, defect and problem.After ultrasonic wave effect Lipsuction instrument developer promotion explanation emphasizes that this technology will not damage normal skin tissue's structure and its blood vessel and nerve etc., but It theoretically analyzes, which is also likely to crush or damage as can the adipocyte of quick damage or broken subcutaneous tissue Normal skin tissue's other structures and functioning cell, it could even be possible to skin histology blood vessel, nerve and cutaneous appendages official rank It causes to damage or destroy.Such as emphasize that this technology will not injured cutaneous tissue structure and its blood vessel by instrument developer promotion explanation With nerve etc., then theoretically analyzing, its damage in practical application to subcutaneous fat cells or broken effect also can be very It is small, it is desirable to realize that the target of a large amount of fragmenting fat cells also will be very slow, and natural absorption of the subcutaneous tissue to fat Produced effect also can be very slow, therefore practical function may be fairly limited.In addition it is available with closely related these three of the present invention The lipsuction after the technical method for extracting subcutis superabundant fats, either ultrasonic wave effect, or electromagnetic effect Lipsuction afterwards, or quickly form Negative-pressure Liposuction, and the key and core of its technology and method all to use it is different Method or means are another after accelerating or promote the damage to greatest extent of subcutaneus adipose tissue and adipocyte or destroying and remove it. But the self superabundant fats tissue and adipocyte extracted from Reasonable Protection and comprehensive utilization from body fat position, particularly, A variety of periphery groups with high bioactivity are extracted and isolated in the autologous adipose tissue and adipocyte valuable from these Cell is knitted (including adipose stromal cells, Preadipocyte, vascular endothelial cell, fibroblast, macrophage, fat source Property stem cell and fat stem cell) and its extracellular matrix components and therefrom obtain fat-derived stem cells (ADSCs) and its institute The living resources of the preciousness such as cytokine profiles and growth factor (VEGF, IGF, HGF, TGF-β, FGF) are secreted, are moulded for human body Shape, body beautification, beauty, improvement or modification facial defects and recess, delay or mitigates skin natural aging and light aging, improvement chest It is angularly said with bung fat Tissue distribution unevenness and depauperation, it is clear that above-mentioned three kinds are presently available for extracting subcutaneous group of human body The technical method for knitting superabundant fats is accelerated or is promoted whole damages of subcutaneous fat cells or destroys and undesirable.In addition, mesh It is preceding to be liposuction for its emphasis of these methods of liposuction and key technology, design is not accounted for completely takes out adipose tissue In fat-derived histocyte, the particularly recycling of fat-derived stem cells and its utilize, it is precious so as to not only cause these The loss of your living resources and waste also result in their serious pollutions to environment.

The content of the invention

（One）The purpose and meaning of utility model patent of the present invention

A kind of practicality of the fat-derived primary stem cell collection piece-rate system of binary channels for human body liposuction of the present invention New patent is exactly directed to and is fed back with foregoing human body liposuction art and fatty granule cell in the closely related technical background of art Existing apparent deficiency, defect and problem, and invent a kind of more science, advanced, novel, practical and more suitable of design Close modern beauty surgical plastic and minimally invasive medical cosmetology and moulding body beautification and skin anti-aging and face rejuvenation application requirement Utensil.This utility model patent of the present invention, it is extra to body fat position both can safely, quickly and effectively to have implemented The problem of accumulation fat and external superfluous accumulation fat extraction, so as to can reach the purpose of lose weight moulding and beauty treatment.Meanwhile It can also greatly reduce or mitigate, can even avoid over pair being be easy to cause in body fat aspiration procedure is carried out with science It artificially damages or destroys caused by subcutaneous tissue and adjacent tissue and internal organs and its generation of complication and complication；It is heavier It wants, this utility model patent of the invention, can also preferably, intactly retain these fat extracted out from human body Natural bioactive of integrality and cells in situ of tissue or adipocyte morphosis etc..Meanwhile it is using the present invention this Kind utility model patent, can carry out the collection of body fat and its cell with separating and integrating profit automatically under aseptic conditions With, and therefrom extracting and developing and prepare human body beauty treatment and body beautification and skin anti-aging and the required matrix of face rejuvenation Vascular components（SVF）Wait various structures and functioning cell；It particularly, can also be from this by this utility model patent of the present invention In a little more complicated human fatty tissues and its cell actively and effective extracting and developing and prepare more precious people from The primary stem cell of body fat source property and fat-derived mescenchymal stem cell and its secreted cell factor and growth factor etc.；And And if primary stem cell fat-derived to these people of acquisition continues to carry out in vitro culture and induced amplification in laboratory, also Purity higher, more, better quality the different fat-derived passage stem cells of algebraically can be obtained.In addition, it is of the invention Utility model patent, can also greatly increase human body liposuction work efficiency, while improve extraction adipose tissue and adipocyte Quality and quantity, and when using it for same individual different parts, realize and reach other heterologous biology filler institutes It is more difficult possess it is easy to operate, prepare easy, safe to use, good biocompatibility, no biotoxicity and abnormal immune reaction, can Repeatability application, effect stability are reliable, there is the look and feel completely the same with normal-fat tissue after transplanting.

（Two）The technical solution of utility model patent of the present invention

1. the operation principle of utility model patent of the present invention：It is to be worked using starting adjustable negative-pressure device first, It causes to form vacuum and negative pressure in human fatty tissue aspirated liquid collecting pipe, so as to by the extra accumulation in subcutis Adipose tissue and its cell component are drawn into human fatty tissue aspirated liquid collecting pipe；Pass through stainless steel human fatty tissue again The several equispaced 5mm's in internal probe periphery of the spheroidal and its spheroidal lower end 50mm of acquisition probe top smooth seal is straight All particle diameters in body fat liquid are more than 70 μm by the round hole of footpath 2mm and the stainless steel cell sieve of 70 μm of sieve diameter Cell, blood vessel, tissue fibers and connective tissue etc. retain completely, and all different type cells for being less than 70 μm are (including fat Primary stem cell of source property etc.) it both passes through；Density is more than by 1.080g/mm by Density Gradient Centrifugation again simultaneously³Special high score is careful The erythroprecipitin of born of the same parents' separation gel is in cell separation gel bottommost, and density is less than 1.080g/mm by Density Gradient Centrifugation³Special height Molecular cell separation gel is then present in including fat-derived primary stem cell in cell separation gel plane；Cell separation is abandoned in pumping After the upper liquid of glue upper strata non-cell components, then sterile tissue digestive juice is passed through into push type and is loaded bottle and its 0.22 μm of bacteria filter It adds in centrifugation cell and is placed in centrifugally operated after 37 constant-temperature tables digest, you can obtain containing fat-derived primary stem cell Cell.

2. the parts of utility model patent of the present invention are formed：It is by an overall length 100mm, internal diameter 25mm, outer diameter The human fatty tissue aspirated liquid collecting pipe (1) for the plastic circular water white transparency that 30mm is marked with volume markings, it is thickness 10mm, close Spend 1.080g/mm³Special high score daughter cell separation gel (2), shape is cone, cone diameter 25mm, height 30mm, sieve pore are straight The stainless steel cell sieve (3) in 70 μm of footpath, human fatty tissue aspirated liquid collecting pipe pipe is interior, stainless steel is supported at tube bottom 60mm The annular fulcrum (4) of cell sieve, the tube wall parallel with plane on cell separation gel protrude outside with connection screw thread, internal diameter The centrate pump orifice (5) of 1.5mm, tube wall parallel with plane in stainless steel cell sieve protrude it is outside, two completely Symmetry, with connection screw thread, internal diameter 1.5mm human fatty tissue pump orifice (6), two internal diameter 1.5mm, outer diameters 2mm, length 200mm water white transparency, can flexible rotating plastic flexible pipe (7) and the plastic flexible pipe both ends with convenient The connector being securely connected with human fatty tissue pump orifice (6) and human fatty tissue acquisition probe (8) screw thread, two internal diameters 1.5mm, outer diameter 2mm, (its tips of probes is the ball of smooth seal to the human fatty tissue acquisition probe (8) of length 260mm There is the round hole of the diameter 2mm of several equispaced 5mm on shape, ball outer diameter 2.5mm, the internal probe periphery of spheroidal lower end 50mm, And the spiral connector of the probe end just can with can flexible rotating plastic flexible pipe (7) exactly match be connected it is colourless Bright plastics hose connector securely connects), the helixseal lid of human fatty tissue aspirated liquid collecting pipe (is covered there are two equal with spiral shell Line, internal diameter be 1.5mm nozzle, one of them be connect negative tube spiral mouth, another then be matching connection 0.22 μm The spiral nozzle of bacteria filter) (9), a length is that 500mm can cover complete of spiral mouth for being connected negative tube with helixseal (the negative-pressure ward flexible plastic tube middle is a sky communicating completely, sphere diameter is 30mm to the negative-pressure ward flexible plastic tube matched somebody with somebody Bulbus cordis shape) (10), an adjustable negative-pressure device (11) that connection is just matched with (10), one just covers with helixseal Connection 0.22 μm of bacteria filter spiral nozzle exactly match and be fully achieved and meet 0.22 μm of membrane type of international quality standards Bacteria filter (12), the push type sample-adding bottle (13) containing 30ml sterile tissue digestive juices in one, one is just loaded with 30ml The sterile emulsion tube and latex pipe clamp (14) that bottle liquid outlet and 0.22 μm of bacteria filter are exactly matched and can securely connected, body fat The prominent red blood cell outside, that with connection screw thread, spout diameter is 20mm in aspirated liquid collecting pipe tube bottom center is organized to centrifuge Separation outlet (15), one just centrifuges outlet spiral with red blood cell that spout diameter is 20mm and exactly matches and coincide Plastic cylindrical water white transparency and include the graduated centrifuge tube of the sterile erythrocyte cracked liquids of 10ml (16), 5 can respectively with from Heart separating liquid pump orifice, human fatty tissue pump orifice, helixseal cover the spiral shell that two nozzle screw threads are exactly matched and coincide Cap (17), 1 just centrifuges the nut for exporting screw thread and exactly matching and coincideing with spout diameter for the red blood cell of 20mm (18), one is used for syringes (19) of the 50ml with syringe needle from centrate pump orifice isolated at suction liquid, fixing human fat The main fixed link (20) of fat tissue aspirated liquid collecting pipe connects the fixed frame pedestal (21) of main fixed link, is used in main fixed link In two clips (22) of fixing human adipose tissue aspirated liquid collecting pipe, the shelf (23) of pipes and tubes is placed, it is fixed main solid The parts such as fixed pole, the fixed frame pallet (24) that can put nut, hose and needle tubing are formed.

3. the parts assembling and connection method of utility model patent of the present invention：The body fat that will be marked with volume markings Organize the injection of aspirated liquid collecting pipe bottom and fixed thickness 10mm, density 1.080g/mm³Special high score daughter cell separation gel, then It is conical, 70 μm of sieve diameter stainless that a shape is fixed on annular fulcrum on the high score daughter cell separation gel at 50mm Steel cell sieve;Meanwhile outside the tube wall of human fatty tissue aspirated liquid collecting pipe and with being put down on special high score daughter cell separation gel The parallel place in face designs and produces a circular open internal diameter 1.5mm and protrudes outside, with connection screw thread centrate suction Mouthful, and, tube wall parallel with plane in stainless steel cell sieve protrude it is outside, two full symmetric property, with connection spiral shell Line, internal diameter 1.5mm human fatty tissue pump orifice.The two full symmetric property, with connection screw thread, internal diameter The human fatty tissue pump orifice of 1.5mm respectively with two internal diameter 1.5mm, outer diameter 2mm, length 200mm can flexible rotating nothing Color transparent plastic hose securely connects, and this two can flexible rotating colourless transparent plastic hose the other end then respectively with two Internal diameter 1.5mm, outer diameter 2mm, the end of stainless steel human fatty tissue acquisition probe of length 260mm are securely connected.This is not Become rusty steel human fatty tissue acquisition probe top be smooth seal spheroidal, ball outer diameter 2.5mm, spheroidal lower end 50mm it There is the round hole of the diameter 2mm of several equispaced 5mm on interior probe periphery, and the spiral connector of the probe end is just It is securely connected with above-mentioned colourless transparent plastic hose connector.In addition, the helixseal lid of human fatty tissue aspirated liquid collecting pipe On two threaded, internal diameter 1.5mm nozzles, one of nozzle is directly connected to the negative-pressure ward flexible plastic exactly matched Pipe (the negative-pressure ward flexible plastic tube middle is a hollow ball shape communicate completely, sphere diameter 30mm) one end, and negative pressure is taken out The other end for inhaling flexible plastic tube is then directly securely connected with matching the adjustable negative-pressure device of connection.And human fatty tissue aspirates One end spiral for 0.22 μm of bacteria filter that another nozzle that the helixseal of liquid collecting pipe covers then directly is exactly matched with it Nozzle securely connects, and the other end of 0.22 μm of bacteria filter is then clamping fixed with sterile emulsion tube and emulsion tube, this sterile emulsion tube The other end then with one in the push type sample-adding bottle outlet connection containing 30ml sterile tissue digestive juices and also latex pipe clamp simultaneously It is fixed.In addition, the tube bottom center outside human fatty tissue aspirated liquid collecting pipe designed and produced protrusion it is outside, with company Red blood cell that connect screw thread, spout diameter 20mm centrifuges outlet, the plastics that just it is exactly matched and coincide with screw thread Cylindrical water white transparency and include the sterile erythrocyte cracked liquids of 10ml graduated centrifuge tube connection.And it is used in order to this The needs of the binary channels fat-derived stem cells acquisition separator of human body liposuction when in use, being also further provided with 5 can distinguish Two nozzle screw threads are covered with centrate pump orifice, human fatty tissue pump orifice, helixseal to exactly match and coincide Nut, and 1 just centrifuge the outlet screw thread nut that exactly matches and coincide with red blood cell, is in addition further provided with one For from the 50ml of centrate pump orifice isolated at suction liquid band needle applicators, and fixing human adipose tissue aspirated liquid The main fixed link of collecting pipe connects the fixed frame pedestal of main fixed link and is taken out in main fixed link for fixing human adipose tissue Two clips of imbibition collecting pipe, place the shelf of pipes and tubes, fixed main fixed link, nut, hose and needle tubing can be put Fixed frame pallet etc..Finally it is fixed together again after putting neatly and packed, and strictly by human injection's utensil It is required that carry out unified sterilization.

The advantageous effect of utility model patent of the present invention

1st, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, the human body that not only can be adapted for different race, sexes and age and different obese degrees carries out fat position Extraction, acquisition and the collection of extra accumulation adipose tissue and adipocyte, moreover, it is also possible to these extractions, acquisition and collect Many cell types for including including fat-derived primary stem cell of human fatty tissue extract, separate and prepare Deng.

2nd, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, it is individually operated both to have can be adapted for one, has been readily applicable to double while simultaneously operating, and it is double while same Step carries out liposuction operation, is not only as many as twice of one man operation's efficiency, and double while synchronous symmetrical suction of fat, takes out Fat is more and liposuction distribution more uniformly, liposuction effect also can be more preferable.

3rd, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, the universal vacuum source of the gas provisioned in common laboratory both can be used or sold using current market adjustable Try the vacuum sources such as vacuum extractor；If moreover, some units for the moment can not solve vacuum source formed negative pressure, can also be straight It connects and replacement operation is carried out using the clinically good syringe pull rod of tightness, it is very convenient and simple, it is also easy to popularize and push away Extensively.

4th, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, using adjustable negative-pressure liposuction technique not only alternative artificial pull rod liposuction utensil at present, but also can be according to facing Bed liposuction is needed in time or suitable for adjusting extracting force and speed, this not only more science and advanced and more effortless and more save labour, Moreover, controllable using the strength size that adjustable negative-pressure aspirates, pumping efficiency can also significantly improve.

5th, the human fatty tissue liquid of the water white transparency with volume markings mark in utility model patent of the invention is taken out Absorb collector least significant end injection and secure thickness be 10mm, density 1.080g/mm³Special high score daughter cell separation Glue,, can will be from human body according to density principle of different sizes when the body fat aspirated liquid after acquisition is centrifuged The adipose tissue liquid Midst density that subcutaneous tissue pumps out is more than 1.080g/mm³The cellular components such as human erythrocyte precipitate automatically Under the cell separation gel, and those density are less than 1.080g/mm in human fatty tissue liquid³Various cellular components (bag Including including fat-derived primary stem cell) it is then naturally occurring on the cell separation gel, it is achieved thereby that centrifuging for the first time It will be by

It is pumped to the red blood cell in external body fat liquid and the various cells including fat-derived primary stem cell Component formation is automatically separated state, so that the progressively separating effect or efficiency of fat tissue cell improve.

6th, the human fatty tissue in utility model patent of the invention in the water white transparency marked with volume markings is taken out Imbibition collecting pipe place parallel with plane on cell separation gel designed and produced tube wall protrude it is outside, with connection screw thread , the centrate pump orifice of internal diameter 1.5mm, while be equipped with the special sterile syringe of a 50ml, it is achieved thereby that For the first time centrifugation can by the liquid fat of those top layers of centrifugation, swelling anaesthesia liquid, rinse physiological saline and those Cell and fragment of tissue of floating etc. are pumped in vitro in time, so as to (fatty to realize or reaching human fatty tissue cell Primary stem cell of source property etc.) later separation efficiency or effect raising lay the foundation.

7th, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, the circular cone in one 70 μm of aperture of placement on the special cell separation gel of human fatty tissue aspirated liquid collecting pipe It is big will effectively can be sucked up to all particle diameters in external body fat liquid in time for shape funnel-form stainless steel cell sieve It is retained completely in 70 μm of cell, blood vessel, tissue fibers and connective tissue etc., and all different type cells for being less than 70 μm (including fat-derived primary stem cell etc.) may pass through cell, and external body fat is sucked up to so as to be furthermore achieved The big cellule of different-diameter is automatically separated again in liquid, also further improves cell separating effect or efficiency.And this The stainless steel cell sieve of kind specified particle diameter is designed to funnel shaped, cell filtration area can not only increased, cell filtration efficiency Higher, and, to being sucked up to cell of all particle diameters more than 70 μm, blood vessel, fascia, tissue in external fat liquor Fiber and connective tissue are when retentions, also less susceptible blocking or delay.

8th, in the utility model patent two carry handle stainless steel human fatty tissue acquisition probe, not only shape set Count original in style, and patient holds handle more convenient operation, is suitble to and application is flexible.Particularly its tips of probes is smooth close Seal spheroidal, and ball outer diameter is more than main body probe diameter, make smooth spheroidal probe can be reduced or avoided to greatest extent into Row subcutaneous puncture and travelling liposuction procedures in may to subcutaneous tissue cell, blood vessel, nerve, muscle, even internal organs equivalent damage and It destroys.And protectiveness spheroidal probe lower end 50mm internal probe periphery designs have several circular apertures, both can ensure that in people These suction holes are not completely plugged during body liposuction, while are also helped the quick, safety after negative pressure is formed and efficiently drawn skin Undertissue is extra or the fat of accumulation, and can play optimal liposuction effect to greatest extent in liposuction procedures.

9th, the human fatty tissue in utility model patent of the present invention in the water white transparency marked with volume markings aspirates The full symmetric binary channels connector in left and right is also specially devised outside the tube wall of liquid collecting pipe, respectively with left and right binary channels spiral The stainless steel adipose tissue acquisition probe afterbody of the matched colourless transparent plastic hose connector of formula connector and two band handles After connection, superabundant fats tissue and adipocyte in suction body are operated simultaneously from body both sides for two medical workers.Once Negative pressure is provided, then the fat liquor of subcutaneous tissue then can be automatically from two wing passages simultaneously into the adipose tissue liquid with volume markings In aspirated liquid collecting pipe, it so can both save or shorten operating time, improve work efficiency, can also make the fat disposably aspirated More faster, and the position for being sucked fat looks evenly and symmetrically liquid.

10th, in utility model patent of the invention, the binary channels fat of a mating human body liposuction is also specially devised The fixing bracket and its parts arranging apparatus of the primary stem cell collection piece-rate system of source property.Fixing bracket can be supported and fixed Human fatty tissue liquid aspirated liquid collecting pipe carries out other motion actions so that the implementer that performs the operation can extract both hands out；And zero Part arranging apparatus is both put and is used in order convenient for the specification of each device, and also allows for that operator is easy to use and sterile package The features such as dress.

11st, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, the head cover of mating human fatty tissue liquid aspirated liquid collecting pipe, and two nozzles one on head cover have also been devised Face connects negative pressure adjusting device, another nozzle can then connect sterilizing filter, can so make to take out into human fatty tissue liquid The cell dissociation buffer of imbibition collecting pipe is automatically injected and can reach sterile working requirement, prevents cell dissociation buffer from entering in pipe When be contaminated.

12nd, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention Also configure steril cell digestive juice with new patent, so as in the case where aseptic condition is fully sealed to human fatty tissue liquid aspirated liquid The various histocytes of collecting pipe carry out digesting and extracting separation repeatedly, make the fat-derived primary of clinical treatment needs Stem cell.

12nd, the reality of the fat-derived primary stem cell collection piece-rate system of this binary channels for human body liposuction of the invention With new patent, originally discarded human body suction of fat can also re-used, not only save valuable living resources, but also Reduce them to external environmental.Meanwhile the utility model patent uses and operates in laboratory natural environment item It is carried out under part, without consuming the energy excessively, and with design science, easy to operate, method specification, safe to use, environmental protection and energy saving The features such as.

Description of the drawings

Fig. 1 is that a kind of fat-derived primary stem cell collection piece-rate system component of binary channels for being used for human body liposuction disperses Figure；

Fig. 2 is that one kind is used for the fat-derived primary stem cell collection piece-rate system constitutional diagram of binary channels of human body liposuction (not Fill fixing device）；

Fig. 3 is a kind of fat-derived primary stem cell collection piece-rate system constitutional diagram of binary channels for being used for human body liposuction（ Fill fixing device）.

All parts especially carry out one using monolithic sequence number in these three attached drawings and its patent declaration material One indicates and explains in detail and illustrate.

(1) be an overall length 100mm, internal diameter 25mm, outer diameter 30mm plastic cylindrical water white transparency body fat group Knit aspirated liquid collecting pipe；(2) be thickness be 10mm, density is the special high score daughter cell separation gels of 1.080g/mm3；(3) it is shape For cone, cone diameter 25mm, be highly 30mm, the stainless steel cell sieve that sieve diameter is 70 μm；(4) it is in human body fat The annular fulcrum of stainless steel cell sieve (3) is supported in the pipe of fat tissue aspirated liquid collecting pipe (1), at tube bottom 60mm；(5) it is The tube wall parallel with cell separation gel plane protrudes outside centrate with connection screw thread, that internal diameter is 1.5mm and takes out Suction inlet；(6) be, tube wall parallel with plane in stainless steel cell sieve protrude it is outside, two full symmetric property, with connection Screw thread, internal diameter be 1.5mm human fatty tissue pump orifice；(7) be two internal diameters be 1.5mm, outer diameter 2mm, length are The water white transparency of 200mm, can flexible rotating plastic flexible pipe and the plastic flexible pipe both ends with convenient with body fat group Knit the connector that pump orifice (6) and human fatty tissue acquisition probe (8) screw thread securely connect；(8) it is that two internal diameters are (its tips of probes is the ball of smooth seal to the human fatty tissue acquisition probe that 1.5mm, outer diameter 2mm, length are 260mm Shape, ball outer diameter are 2.5mm, and there is the circle of a diameter of 2mm of several equispaced 5mm on the probe periphery within the 50mm of spheroidal lower end Shape hole, and the spiral connector of the probe end just can with can the plastic flexible pipe (7) of flexible rotating exactly match what is be connected Colourless transparent plastic hose connector securely connects)；(9) be human fatty tissue aspirated liquid collecting pipe (1) helixseal lid (lid Threaded, internal diameter is the nozzle of 1.5mm there are two upper, one of them is the spiral mouth for connecting negative tube, another is then The spiral nozzle of matching 0.22 μm of bacteria filter of connection）；(10) it is that a length can be connected for 500mm on helixseal lid (9) (the negative-pressure ward flexible plastic tube middle is a complete phase to the negative-pressure ward flexible plastic tube that the spiral mouth of negative tube exactly matches The hollow ball shape logical, sphere diameter is 30mm)；(11) it is an adjustable negative-pressure device that connection is just matched with (10)；(12) It it is one just with being connected that the spiral nozzle of 0.22 μm of bacteria filter exactly matches on helixseal lid (9) and being fully achieved simultaneously Meet 0.22 μm of membrane type bacteria filter of international quality standards；(13) it is push type containing 30ml sterile tissue digestive juices in one It is loaded bottle；(14) it is one and the nothing that bottle liquid outlet and 0.22 μm of bacteria filter are exactly matched and can be securely connected just is loaded with 30ml Bacterium emulsion tube and latex pipe clamp；(15) it is that human fatty tissue aspirated liquid collecting pipe (1) tube bottom center is protruded outwards, with even Red blood cell that connect screw thread, that spout diameter is 20mm centrifuges outlet；(16) it is just 20mm with spout diameter to be one Red blood cell centrifuges outlet (15) spiral and exactly matches and coincide plastic cylindrical water white transparency and to include 10ml sterile The graduated centrifuge tube of erythrocyte cracked liquid；(17) be totally 5 can respectively with centrate pump orifice (5), human fatty tissue The nut that pump orifice (6), the upper two nozzle screw threads of helixseal lid (9) are exactly matched and coincide；(18) be 1 just with nozzle The red blood cell that internal diameter is 20mm centrifuges the nut that outlet (15) screw thread is exactly matched and coincide；(19) be one be used for from from The syringes of the 50ml with syringe needle of heart separating liquid pump orifice (5) isolated at suction liquid；(20) it is fixing human adipose tissue aspirated liquid The main fixed link of collecting pipe (1)；(21) it is the fixed frame pedestal that connects main fixed link；(22) it is to be used to fix in main fixed link Two clips of human fatty tissue aspirated liquid collecting pipe (1)；(23) be place pipes and tubes shelf；(24) it is fixed main solid Fixed pole, fixed frame pallet that nut, hose and needle tubing can be put.

Specific embodiment

The binary channels that can be used for human body liposuction with reference to Figure of description one kind that the present invention will be described in detail is fat-derived The specific embodiment of the utility model patent of primary stem cell collection piece-rate system.

1. manufacture and design the parts of utility model patent of the present invention：One overall length 100mm, internal diameter 25mm, outer diameter 30mm The human fatty tissue aspirated liquid collecting pipe (1) of plastic circular water white transparency with volume markings mark, thickness 10mm, density 1.080g/mm³Special high score daughter cell separation gel (2), shape is cone, cone diameter 25mm, height 30mm, sieve diameter 70 μm of stainless steel cell sieve (3), human fatty tissue aspirated liquid collecting pipe pipe is interior, supports stainless steel thin at tube bottom 60mm The annular fulcrum (4) of born of the same parents' sieve, the tube wall parallel with plane on cell separation gel protrude outside with connection screw thread, internal diameter The centrate pump orifice (5) of 1.5mm, tube wall parallel with plane in stainless steel cell sieve protrude it is outside, two completely Symmetry, with connection screw thread, internal diameter 1.5mm human fatty tissue pump orifice (6), two internal diameter 1.5mm, outer diameters 2mm, length 200mm water white transparency, can flexible rotating plastic flexible pipe (7) and the plastic flexible pipe both ends with convenient The connector being securely connected with human fatty tissue pump orifice (6) and human fatty tissue acquisition probe (8) screw thread, two internal diameters 1.5mm, outer diameter 2mm, (its tips of probes is the ball of smooth seal to the human fatty tissue acquisition probe (8) of length 260mm There is the circle of the diameter 2mm of several equispaced 5mm on shape, the probe periphery within ball outer diameter 2.5mm, spheroidal lower end 50mm Hole, and the spiral connector of the probe end just can with can the plastic flexible pipe (7) of flexible rotating exactly match the nothing being connected Color transparent plastic hose connector securely connects；The helixseal lid of human fatty tissue aspirated liquid collecting pipe is (there are two covering Threaded, internal diameter is the nozzle of 1.5mm, one of them is the spiral mouth for connecting negative tube, another is then matching connection The spiral nozzle of 0.22 μm of bacteria filter) (9), a length is that 500mm can cover the spiral mouth for being connected negative tube with helixseal (the negative-pressure ward flexible plastic tube middle is that one communicating completely, sphere diameter is to the negative-pressure ward flexible plastic tube exactly matched The hollow ball shape of 30mm) (10), one just matched with (10) connection adjustable negative-pressure device (11), one just with spiral The spiral nozzle of 0.22 μm of bacteria filter of connection exactly matches on sealing cover, and is fully achieved and meets international quality standards 0.22 μm of membrane type bacteria filter (12), the push type sample-adding bottle (13) containing 30ml sterile tissue digestive juices in one, one just The sterile emulsion tube and latex pipe clamp that exactly matches and can securely be connected with 30ml sample-adding bottle liquid outlets and 0.22 μm of bacteria filter (14), human fatty tissue aspirated liquid collecting pipe tube bottom center is protruded outwards, with connection screw thread, spout diameter 20mm Red blood cell centrifuge outlet (15), one just with spout diameter be 20mm red blood cell centrifuge outlet spiral it is complete Matching and identical graduated centrifuge tube (16) that is plastic cylindrical water white transparency and including the sterile erythrocyte cracked liquids of 10ml, 5 It is a to cover two complete of nozzle screw threads with centrate pump orifice, human fatty tissue pump orifice, helixseal respectively The nut (17) matched somebody with somebody and coincide, 1 just with spout diameter be 20mm red blood cell centrifuge outlet screw thread exactly match with Identical nut (18), one is used for syringes (19) of the 50ml with syringe needle from centrate pump orifice isolated at suction liquid, The main fixed link (20) of fixing human adipose tissue aspirated liquid collecting pipe connects the fixed frame pedestal (21) of main fixed link, in master Two clips (22) of fixing human adipose tissue aspirated liquid collecting pipe are used in fixed link, place the shelf of pipes and tubes (23), the parts such as fixed frame pallet (24) fixing main fixed link, can putting nut, hose and needle tubing are formed.

2. it is assembled according to the parts of design and assembly utility model patent of the present invention with being connected：One kind for human body liposuction it The fat-derived primary each parts connection features of stem cell collection piece-rate system of binary channels:This human body with volume markings mark The injection of adipose tissue aspirated liquid collecting pipe lowermost end and fixed thickness 10mm, density 1.080g/mm³Special high score daughter cell separation Glue；And on the annular fulcrum on the special high score daughter cell separation gel at plane 50mm sealing fix a shape for cone, Cone diameter 25mm, height 30mm, the stainless steel cell sieve of 70 μm of sieve diameter;Meanwhile it is received in human fatty tissue aspirated liquid Outside the tube wall of collector and place parallel with plane on special high score daughter cell separation gel designs and produces a circular open internal diameter 1.5mm protrudes outside, with connection screw thread centrate pump orifice, and parallel with plane in stainless steel cell sieve , tube wall protrude outside, two full symmetric property, human fatty tissue suction with connection screw thread, internal diameter 1.5mm Mouthful.The two full symmetric property, with connection screw thread, internal diameter 1.5mm human fatty tissue pump orifices respectively with two Internal diameter 1.5mm, outer diameter 2mm, length 200mm can the colourless transparent plastic hose of flexible rotating securely connect, and this two can spirit The other end of the colourless transparent plastic hose rotated living then respectively with two internal diameter 1.5mm, outer diameter 2mm, length 260mm it is stainless The end of steel human fatty tissue acquisition probe is securely connected.The top of the stainless steel human fatty tissue acquisition probe is smooth There is the diameter of several equispaced 5mm on the spheroidal of sealing, the probe periphery within ball outer diameter 2.5mm, spheroidal lower end 50mm The round hole of 2mm, and the spiral connector of the probe end is just securely connected with above-mentioned colourless transparent plastic hose connector. In addition, two threaded, internal diameter 1.5mm nozzles that the helixseal of human fatty tissue aspirated liquid collecting pipe covers, In a nozzle be directly connected to the negative-pressure ward flexible plastic tube exactly matched (the negative-pressure ward flexible plastic tube middle for one it is complete Hollow ball shape communicate entirely, sphere diameter 30mm) one end, and the other end of negative-pressure ward flexible plastic tube is then directly with matching connection Adjustable negative-pressure device securely connects.And another nozzle that the helixseal of human fatty tissue aspirated liquid collecting pipe covers is then One end spiral nozzle directly with its 0.22 μm of bacteria filter exactly matched is securely connected, and the other end of 0.22 μm of bacteria filter is then used Sterile emulsion tube and emulsion tube are clamping fixed, and the other end of this sterile emulsion tube in one then with containing 30ml sterile tissue digestive juices Push type sample-adding bottle outlet connection and also simultaneously emulsion tube it is clamping fixed.In addition, outside human fatty tissue aspirated liquid collecting pipe Tube bottom center has designed and produced that protrusion is outside, red blood cell centrifugation with connection screw thread, spout diameter 20mm Outlet, just it is exactly matched and coincide with screw thread plastic cylindrical water white transparency and includes the sterile red blood cells of 10ml The graduated centrifuge tube connection of lysate.And for this binary channels fat-derived stem cells acquisition point for human body liposuction From the needs of device when in use, being also further provided with 5 can aspirate with centrate pump orifice, human fatty tissue respectively Mouth, helixseal cover the nut that two nozzle screw threads are exactly matched and coincide, and 1 just centrifuges outlet with red blood cell (15) in addition the nut that screw thread is exactly matched and coincide is further provided with one and is used for from centrate pump orifice isolated at suction liquid Syringes of the 50ml with syringe needle, and the main fixed link of fixing human adipose tissue aspirated liquid collecting pipe connects main fixed link Fixed frame pedestal and in main fixed link be used for fixing human adipose tissue aspirated liquid collecting pipe two clips, place it is all kinds of The shelf of pipe, fixed frame pallet that fix main fixed link, that nut, hose and needle tubing can be put etc..Finally, another and fixation pendulum Put it is neat after packed, and strictly by human injection with utensil requirement carry out uniformly sterilization it.

The specifically used method of utility model patent of the present invention：(1) it is stringent by medical treatment and nursing conventional specification requirement, entirely Preparation of the journey before aseptic operating room first carries out human body liposuction by the qualified medical staff of tool, i.e., it is numb after first being sterilized to operation receiveing person Liquor-saturated, then attending physician cuts an osculum drafting liposuction area skin；(2) will sterilize and through qualified sterile human body is examined to take out The fat-derived primary stem cell collection piece-rate system packaging of binary channels of fat is opened, the careful careful assembling according to codes and standards It is attached, and is placed and secured in the fixing device of Aided design with connection method；(3) carefully human fatty tissue is gathered The matched water white transparency casing of probe removes, by qualified medical staff by top be spheroidal stainless steel human body fat Fat tissue sampling probe is carefully gently inserted into subcutaneus adipose tissue layer from the skin incision for intending liposuction position；(4) according in advance Designed liposuction amount opens adjustable negative-pressure aspirator, slowly gently gradual mobile human body adipose tissue acquisition Probe is until reach required body fat and adipocyte total amount；(5) slowly softly human fatty tissue is gathered on one side and visited Pin takes out, and by the conventional careful careful progress wound suture of medical treatment and nursing, disinfection, wrapping and antimicrobial treatment, while remove negative pressure Connecting tube and two stainless steel human fatty tissue acquisition probes, and immediately by these connectors respectively with matched threaded Sterile nut secure seal covers；(6) after the collecting pipe symmetrical balance weight for acquiring human fatty tissue aspirated liquid by two, Be immediately placed in centrifuge, then with 1000rpm/min centrifuge 10min after, take out this with volume markings mark body fat group Knit the collecting pipe of aspirated liquid；(7) the centrate pump orifice nut parallel with cell separation gel plane is unscrewed immediately, and it is rapid It is carefully inhaled with asepsis injector and abandons after upper liquid tightening nuts again；(8) connection immediately includes the pressing of 30ml sterile tissue digestive juices Formula be loaded bottle, according to the scale on human fatty tissue aspirated liquid collecting pipe understand the pipe in remaining cell liquid accumulate, Ran Houzai Push type is loaded in bottle digestive juice of the aseptic process again through bacteria filter filtering in proportion to be added thereto；(9) sample-adding bottle is removed Connection and matching nut is tightened, abundant mixing is placed on rotation or vibration digestion 30-50min in 37 DEG C of constant-temperature tables；⑽ The human fatty tissue aspirated liquid collecting pipe is taken out, will be centrifuged again after its symmetrical balance weight, centrifugal rotational speed 1000rpm/ Min, centrifugation 10min take out after closing centrifugation electromechanical source, unscrew the centrate parallel with cell separation gel plane again and take out The nut of suction inlet, and after carefully being inhaled with asepsis injector rapidly abandon supernatant liquid again, then nut is screwed on immediately；(11) so repeatedly After 1-2 times, then the cell liquid that has digested of part suctioned out with very thin aseptic straw by centrate pump orifice, a part into Row fat-derived stem cells secondary culture, remainder include fat-derived primary stem cell etc. and all sterile are inhaled with syringe Injection transplantation is used for after net；(12) human fatty tissue aspirated liquid collecting pipe centrifugation is contained into erythrocyte splitting to bottommost again The graduated centrifuge tube of liquid is opened, and is all suctioned out it and is applied to face in a manner of non-injection as skin anti-aging and face youth Change.

Implementation example described in the utility model patent of the present invention, only to the specific embodiment institute of this patent product The detailed description of progress is not that design to the utility model patent and its scope are defined.Therefore, this hair is not being departed from On the premise of bright utility model patent design philosophy, scientific research scholar and biotechnology personnel couple in all association areas The various modifications change and improve improvement etc. that the technical solution of the utility model patent of the present invention is done, should all fall into the present invention's The protection domain of utility model patent, and the claimed technology contents of utility model patent of the present invention, are all recorded In detail in the claims.

Claims (2)

1. one kind is used for the fat-derived primary stem cell collection piece-rate system of binary channels of human body liposuction, it is characterised in that：It is By the body fat group of an overall length 100mm, internal diameter 25mm, outer diameter 30mm the plastic circular water white transparency marked with volume markings Knit aspirated liquid collecting pipe (1), thickness 10mm, density 1.080g/mm³Special high score daughter cell separation gel (2), shape is circular cone Shape, cone diameter 25mm, height 30mm, the stainless steel cell sieve (3) of 70 μm of sieve diameter, human fatty tissue aspirated liquid are collected The annular fulcrum (4) that stainless steel cell sieve (3) is supported in (1) pipe, at tube bottom 60mm is managed, is put down with plane on cell separation gel Capable tube wall protrude it is outside with connection screw thread, internal diameter 1.5mm centrate pump orifice (5), with stainless steel cell Plane is parallel on sieve, tube wall protrudes outside, two full symmetric property, human body with connection screw thread, internal diameter 1.5mm Adipose tissue pump orifice (6), two internal diameter 1.5mm, outer diameter 2mm, length 200mm water white transparency, can flexible rotating plastics The both ends of hose (7) and the plastic flexible pipe are gathered with convenient with human fatty tissue pump orifice (6) and human fatty tissue The connector that probe (8) screw thread securely connects, the human fatty tissue acquisition of two internal diameter 1.5mm, outer diameter 2mm, length 260mm Probe (8), tips of probes are the spheroidal of smooth seal, the probe periphery within ball outer diameter 2.5mm, spheroidal lower end 50mm There is the round hole of the diameter 2mm of several equispaced 5mm, and the spiral connector of the probe end just can be with can flexibly turn The colourless transparent plastic hose connector that dynamic plastic flexible pipe (7) exactly matches connection securely connects；Human fatty tissue aspirated liquid The helixseal lid (9) of collecting pipe (1) is covered there are two the nozzle that threaded, internal diameter is 1.5mm, one of them is to connect The spiral mouth of negative tube is connect, another is then the spiral nozzle of matching 0.22 μm of bacteria filter of connection, and length can be with for 500mm The negative-pressure ward flexible plastic tube (10) that the spiral mouth of negative tube exactly matches is connected on helixseal lid (9), the negative-pressure ward is soft Plastic tube middle is a hollow ball shape communicating completely, sphere diameter is 30mm, one just with negative-pressure ward flexible plastic tube (10) the adjustable negative-pressure device (11) of matching connection, one is just connected 0.22 μm of bacteria filter on helixseal lid (9) It is that spiral nozzle exactly matches and be fully achieved and meet 0.22 μm of membrane type bacteria filter (12) of international quality standards, in one Push type sample-adding bottle (13) containing 30ml sterile tissue digestive juices, one is just loaded bottle liquid outlet and 0.22 μm of filter with 30ml The sterile emulsion tube and latex pipe clamp (14) that bacterium device is exactly matched and can securely connected, human fatty tissue aspirated liquid collecting pipe (1) the prominent red blood cell outside, that with connection screw thread, spout diameter is 20mm in tube bottom center centrifuges outlet (15), One just centrifuges the plastic cylinder for exporting (15) spiral and exactly matching and coincideing with spout diameter for the red blood cell of 20mm Shape water white transparency and include the graduated centrifuge tube of the sterile erythrocyte cracked liquids of 10ml (16), totally 5 can respectively with centrifugation Liquid pump orifice (5), human fatty tissue pump orifice (6), helixseal lid (9) upper two nozzle screw threads are exactly matched and coincide Nut (17), 1 just centrifuges outlet (15) screw thread with red blood cell that spout diameter is 20mm and exactly matches and coincide Nut (18), one is used for syringes (19) of the 50ml with syringe needle from centrate pump orifice (5) isolated at suction liquid, fixed The main fixed link (20) of human fatty tissue aspirated liquid collecting pipe (1) connects the fixed frame pedestal (21) of main fixed link, main solid Two clips (22) of fixing human adipose tissue aspirated liquid collecting pipe (1) are used in fixed pole, place the shelf of pipes and tubes (23), fixed frame pallet (24) parts fixing main fixed link, can putting nut, hose and needle tubing are formed.

2. the fat-derived primary stem cell collection piece-rate system of the binary channels according to claim 1 for human body liposuction, It is characterized in that：The lowermost end of this human fatty tissue aspirated liquid collecting pipe with volume markings mark injects and secures thickness Spend 10mm, density 1.080g/mm³Special high score daughter cell separation gel；And the plane on the special high score daughter cell separation gel Sealing secures a shape as cone, cone diameter 25mm, height 30mm, sieve diameter 70 on annular fulcrum at 50mm μm stainless steel cell sieve；Meanwhile divide outside the tube wall of human fatty tissue aspirated liquid collecting pipe and with special high score daughter cell Place parallel from plane on glue designs and produces a circular open internal diameter 1.5mm and protrudes outside, with connection screw thread centrifugation point Chaotropic pump orifice and, tube wall parallel with plane in stainless steel cell sieve protrude outside, two full symmetric property, band There is connection screw thread, internal diameter 1.5mm human fatty tissue pump orifice, the two full symmetric property, with connection screw thread, The human fatty tissue pump orifice of internal diameter 1.5mm respectively can flexible rotating with two internal diameter 1.5mm, outer diameter 2mm, length 200mm Colourless transparent plastic hose securely connect, and this two can the other end of colourless transparent plastic hose of flexible rotating then distinguish Securely it is connected with the end of stainless steel human fatty tissue acquisition probe of two internal diameter 1.5mm, outer diameter 2mm, length 260mm, The top of the stainless steel human fatty tissue acquisition probe be smooth seal spheroidal, ball outer diameter 2.5mm, spheroidal lower end There are the round hole of the diameter 2mm of several equispaced 5mm, and the spiral connection of the probe end in probe periphery within 50mm Mouth is just securely connected with above-mentioned colourless transparent plastic hose connector, in addition, the spiral of human fatty tissue aspirated liquid collecting pipe Two threaded, internal diameter 1.5mm nozzles on sealing cover, one of nozzle are directly connected to the negative-pressure ward exactly matched Flexible plastic tube one end, which is a hollow ball shape communicate completely, sphere diameter 30mm, and is born The other end of pressure suction flexible plastic tube is then directly securely connected with matching the adjustable negative-pressure device of connection, and human fatty tissue The one end for 0.22 μm of bacteria filter that another nozzle that the helixseal of aspirated liquid collecting pipe covers then directly is exactly matched with it Spiral nozzle securely connects, and the other end of 0.22 μm of bacteria filter is then clamping fixed with sterile emulsion tube and emulsion tube, this sterile latex The other end of pipe then with one in the push type sample-adding bottle outlet connection containing 30ml sterile tissue digestive juices and also latex simultaneously Pipe clamp is fixed, in addition, outside human fatty tissue aspirated liquid collecting pipe tube bottom center designed and produced protrusion outwards, band Red blood cell that have connection screw thread, spout diameter 20mm centrifuges outlet, and just it is exactly matched and coincide with screw thread Plastic cylindrical water white transparency and include the sterile erythrocyte cracked liquids of 10ml graduated centrifuge tube connection, moreover, in order to this The needs of separator when in use are gathered for the binary channels fat-derived stem cells of human body liposuction, have also been further provided with 5 energy Covered respectively with centrate pump orifice, human fatty tissue pump orifice, helixseal two nozzle screw threads exactly match and Identical nut and 1 nut for just exactly matching and coincideing with red blood cell centrifugation outlet (15) screw thread, in addition, also One is equipped with to be used for from syringes of the 50ml with syringe needle of centrate pump orifice isolated at suction liquid and fixing human fat The main fixed link of fat tissue aspirated liquid collecting pipe connects the fixed frame pedestal of main fixed link and is used to fix people in main fixed link Two clips of body adipose tissue aspirated liquid collecting pipe, place the shelf of pipes and tubes, fixed main fixed link, nut can be put, The fixed frame pallet of hose and needle tubing.