CN103212125B - A kind of method of rapid extraction rich platelet high-concentration blood plasma and special equipment - Google Patents
A kind of method of rapid extraction rich platelet high-concentration blood plasma and special equipment Download PDFInfo
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Abstract
The method of rapid extraction rich platelet high-concentration blood plasma and a special equipment, belong to rich platelet high-concentration blood plasma extracting method and special equipment technical field.Comprise: 1) by blood from patient's withdraw to injecting the bloodletting tube of anticoagulant; 2) with moving slurry pipe, blood being moved on in centrifuge tube again, adding agglutinant, putting centrifuge tube into centrifuge, with the centrifugal 4-6 minute of rotating speed 2500-3500rpm; 3) then supernatant and platelet rich plasma layer is extracted, with the centrifugal 8-12 minute of rotating speed 1200-1800rpm, cumulative volume in centrifuge tube 3/4? the supernatant of-4/5 is removed, and remaining is rich platelet high-concentration blood plasma.The method of above-mentioned a kind of rapid extraction rich platelet high-concentration blood plasma and special equipment, it is simple to operate, and extracting method yield is high, and obtained rich platelet high-concentration blood plasma quality is good, significantly improves therapeutic effect; And this special equipment structure is simple, easy to use.
Description
Technical field
The invention belongs to rich platelet high-concentration blood plasma (PRP) extracting method and special equipment technical field, be specially a kind of method and special equipment of rapid extraction rich platelet high-concentration blood plasma.
Background technology
Rich platelet high-concentration blood plasma (PlateletRichPlasma, PRP) is the thrombocyte plasma containing high concentration obtained by centrifugal blood.The platelet of human body is under high concentration state, protein (the fibrin with cell adhesion function can be produced, fibronectin & vitronectin), Chinese: fibronectin, FN, and when pH value drops to 6.5-6.7 from 7.0-7.2, add suitable calcium chloride, platelet can be promoted to secrete in a large number have to promote 9 kinds of somatomedin of wound, organization healing and cell regeneration, so PRP is also called " being rich in somatomedin blood plasma ".PRP has the effect of rapid hemostasis, pain relieving, accelerating wound healing, can alleviate the formation of scar after the operation by high degree.In addition, because PRP extracts from patient's self-blood, after Extraction and enrichment process, can be used for the treatment of relevant disease, thus abundance, draw materials conveniently, preparation method is simple and can allow body absorption.Self PRP uses and the problem of virus disseminating and immunologic rejection can be avoided to occur.Therefore from middle nineteen nineties in last century, be widely used in various surgical operation, operation on heart and plastic operation, be also widely used in medical cosmetology aspect at present.So the extraction quality of PRP, it is the key factor affecting its function and effect.
At present, PRP preparation method obviously simplifies, and under household condition namely can prepare in operating room, but must keep aseptic in centrifugal process, and is separated under the high concentration not having thrombocytolysis or destruction, and PRP result prepared by different PRP instrument is different.Some PRP instrument can not produce sufficient amount, the concentrated platelet of Promotive union, this can explain many about using the research that PRP is invalid.Real PRP should be autologous, because foreign body blood platelet does not have vigor, and can not secrete biological activity somatomedin, and its cell membrane is also antigen, and body can be stimulated to produce antibody.PRP preparation method is roughly divided into two classes.One class, by manual operations, utilizes the simple instruments such as centrifuge to prepare under common blood bank conditions, be easy to carry out, but operating procedure is comparatively loaded down with trivial details.Another kind method is use special blood separation instrument to be automatically separated preparation PRP, this method is simply rapid, the blood constituent be left can be fed back in patient body in time, but this easily activates platelet after separation, make the Quality Down of PRP, finally cause therapeutic effect not good.
Summary of the invention
For the above-mentioned problems in the prior art, the object of the invention is to design provides a kind of method of rapid extraction rich platelet high-concentration blood plasma simple to operate and the technical scheme of special equipment, adopt this extracting method yield high, the quality of rich platelet high-concentration blood plasma is good, significantly improves therapeutic effect.
The method of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that comprising the following steps:
1) with bloodletting tube by blood from patient's withdraw to bloodletting tube, first inject anticoagulant in bloodletting tube, the volume of anticoagulant and whole blood is 1:8-10; Described anticoagulant is made up of 10% sodium citrate and 90%EDTA;
2) with moving slurry pipe, blood is moved on in centrifuge tube from bloodletting tube, then add agglutinant at centrifuge tube, make it form gel, the volume of agglutinant and whole blood is 1:8-9, and described agglutinant is made up of 10% calcium chloride and 90% thrombin again; Put centrifuge tube into centrifuge again, with the centrifugal 4-6 minute of rotating speed 2500-3500rpm, blood is divided into 3 layers, bottom is the maximum erythrocyte of sedimentation coefficient, the superiors are supernatant and platelet-poor plasma, and both have skim by intersection, and this thin layer is platelet rich plasma layer;
3) then supernatant and platelet rich plasma layer is extracted, avoid extracting erythrocyte as far as possible, change centrifugal force recentrifuge, with the centrifugal 8-12 minute of rotating speed 1200-1800rpm, blood is divided into 2 layers, and upper strata is supernatant, and lower floor is platelet rich plasma layer, the supernatant of cumulative volume 3/4-4/5 in centrifuge tube is removed, and remaining is rich platelet high-concentration blood plasma.
The method of described a kind of rapid extraction rich platelet high-concentration blood plasma, it is characterized in that: the rich platelet high-concentration blood plasma of acquisition is concentrated further, recentrifuge is separated, rotating speed is with the centrifugal 10-15 minute of 1000-1400rpm, the upper strata of cumulative volume 1/4-1/5 in centrifuge tube is removed, and remaining is concentrated rich platelet high-concentration blood plasma.
The method of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that step 2) in: the rotating speed 2800-3000rpm of centrifuge, centrifugation time is 5 minutes.
The method of described a kind of rapid extraction rich platelet high-concentration blood plasma, it is characterized in that in step 3): the rotating speed 1400-1600rpm of centrifuge, centrifugation time is 9-10 minute.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that comprising centrifuge tube, moves slurry pipe, bloodletting tube;
Described centrifuge tube comprises body, and body top is equipped with seal cover, inlet porting on seal cover, and body middle and upper part arranges thin neck;
The described slurry pipe that moves is made up of sleeve pipe, push rod cooperation, and push rod front end is equipped with sealing ring, and push rod is sealed by sealing ring and internal surface of sleeve pipe and is slidably matched, and sleeve pipe front end arranges interface;
Described bloodletting tube main body is made up of a hollow sleeve, and hollow sleeve arranges the handle of protrusion topmost, handle is arranged two or three connectors;
During use, move the slurry interface of pipe and be connected with the connector of bloodletting tube, the blood in bloodletting tube is extracted into and moves in slurry pipe, and then be connected moving the slurry interface of pipe with the entrance of centrifuge tube, promote push rod and blood is moved on in centrifuge tube.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that: the thin neck outer wall of body middle and upper part is equipped with safe wing.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that: on the outer wall of body bottom, socket arranges protective sleeve.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that: body wall body middle and lower part arranges control reference line, and wall body top arranges overall control line.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that: described sleeve pipe tube wall arranges graduation mark.
The special equipment of described a kind of rapid extraction rich platelet high-concentration blood plasma, is characterized in that: described hollow sleeve surface arranges scale mark.
Described EDTA is ethylenediaminetetraacetic acid, is a kind of important chelating agent.EDTA has many uses, and can be used as the bleach-fixing liquid of color sensitive material washing processing, dyeing assistant, fiber treatment auxiliary agent, cosmetics additive, blood anticoagulant, detergent, stabilizing agent, synthetic rubber polymerization initiator, and EDTA is the representative substances of chelating agen.Energy and alkali metal, rare earth element and transition metal etc. form stable water soluble complex.
10% described sodium citrate refers to containing 10g sodium citrate in 100mL solution, and 90%EDTA refers to containing 90gEDTA in 100mL solution, and 10% calcium chloride refers to containing 10g calcium chloride in 100mL solution, and 90% thrombin refers in 100mL solution containing 90g thrombin.
The method of above-mentioned a kind of rapid extraction rich platelet high-concentration blood plasma and special equipment, it is simple to operate, extracting method yield is high, containing the leukocyte of higher concentration in obtained rich platelet high-concentration blood plasma, high degree reduces infection probability, so doctor just can not carry out beauty treatment injection for curing to patient in operating room, use safety, convenience, therapeutic effect is remarkable; And this special equipment structure is simple, easy to use, just can produce the rich platelet high-concentration blood plasma being rich in high concentration platelet and somatomedin in doctor's office or in operating room in one hour, solve other method fabrication cycle long, easily infect, PC is low, do not reach maximum effectiveness and the hardware facility made and place is subject to the shortcomings such as strict restriction.
Accompanying drawing explanation
Fig. 1 is the structural representation of the centrifuge tube related in the present invention;
Fig. 2 is the structural representation moving slurry pipe related in the present invention;
Fig. 2 is the structural representation of the bloodletting tube related in the present invention;
Fig. 4 is that the using state of the special equipment related in the present invention is with reference to figure mono-;
Fig. 5 is that the using state of the special equipment related in the present invention is with reference to figure bis-;
In figure: 1-centrifuge tube, 2-move slurry pipe, 3-bloodletting tube, 101-entrance, 102-seal cover, 103-overall control line, 104-body, the thin neck of 105-, 106-control reference line, 107-safe wing, 108-protective sleeve, 201-push rod, 202-sleeve pipe, 203-graduation mark, 204-interface, 301-connector, 302-handle, 303-scale mark, 304-hollow sleeve.
Detailed description of the invention
Below in conjunction with Figure of description, the utility model is described in further detail.
As shown in the figure, the method for this rapid extraction rich platelet high-concentration blood plasma, comprises the following steps:
1) use bloodletting tube 3 by blood from patient's withdraw to bloodletting tube 3, first inject anticoagulant in bloodletting tube 3, the volume of anticoagulant and whole blood is 1:8-10; Described anticoagulant is made up of 10% sodium citrate and 90%EDTA;
2) with moving slurry pipe 2, blood being moved on in centrifuge tube 1 from bloodletting tube 3 again, then adding agglutinant at centrifuge tube 1, making it form gel, cementation can be played, somatomedin is limited in coagulation block simultaneously and not easily runs off, be conducive to it and play a role, and be easily shaped; The volume of agglutinant and whole blood is 1:8-9, and described agglutinant is made up of 10% calcium chloride and 90% thrombin; Put centrifuge tube 1 into centrifuge again, with the centrifugal 4-6 minute of rotating speed 3000rpm, blood is divided into 3 layers, bottom is the maximum erythrocyte of sedimentation coefficient, the superiors are supernatant and platelet-poor plasma (platelet-poorplasma, ppp), and both intersections have skim (buffycoat), general naked eyes are not easily found out, this thin layer is platelet rich plasma layer;
3) then supernatant and platelet rich plasma layer is extracted, avoid extracting erythrocyte as far as possible, change centrifugal force recentrifuge, with the centrifugal 8-12 minute of rotating speed 1200-1800rpm, blood is divided into 2 layers, and upper strata is supernatant, and lower floor is platelet rich plasma layer, the supernatant of cumulative volume 3/4-4/5 in centrifuge tube 1 is removed, and remaining is rich platelet high-concentration blood plasma.
In said method step 2) in rotating speed 2500rpm, 2600rpm, 2800rpm, 3200rpm or 3500rpm of centrifuge, 1200rpm, 1400rpm, 1600rpm or 1800rpm in step 3), other step is the same, also can obtain beneficial effect of the present invention.
Go for concentrated rich platelet high-concentration blood plasma, the rich platelet high-concentration blood plasma of acquisition can be concentrated further, recentrifuge is separated, rotating speed is with the centrifugal 10-15 minute of 1000-1400rpm, the upper strata of cumulative volume 1/4-1/5 in centrifuge tube 1 is removed, remaining is concentrated rich platelet high-concentration blood plasma, and PC can be made to be increased to 16 times of whole blood further.
The special equipment of above-mentioned rapid extraction rich platelet high-concentration blood plasma, comprises centrifuge tube 1, moves slurry pipe 2, bloodletting tube 3.Described centrifuge tube 1 comprises body 104, and body 104 top is equipped with seal cover 102, inlet porting 101 on seal cover 102, and body 104 middle and upper part arranges thin neck 105, after adopting thin neck 105, can extract buffy coat more accurately; Thin neck 105 outer wall of body 104 middle and upper part is equipped with safe wing 107, enhances the intensity of thin neck 105, greatly reduce the probability that thin neck 105 is destroyed.On the outer wall of body 104 bottom, socket arranges the soft protective sleeve of one deck 108, and protective sleeve 108 plays dual function that is anti-skidding and protection body 104.Body 104 wall body middle and lower part arranges and controls reference line 106, and wall body top arranges overall control line 103, PRP after the separation in each stage, reasonably can control according to consumption.Described centrifuge tube is typically provided to 15ml, be provided with the overall control line 103 of 15ml topmost, the total blood volume that centrifuge tube is 15ml can be controlled accurately 103 times at overall control line, upper end arranges seal cover 102, ensure that aseptic effect, covering out an entrance 101, allow the insertion moving slurry pipe 2 front end, this centrifuge tube 1 is applicable to various centrifuge, strong adaptability.
The described slurry pipe 2 that moves is made up of sleeve pipe 202, push rod 201 cooperation, push rod 201 front end is equipped with sealing ring, push rod 201 is slidably matched by sealing ring and sleeve pipe 202 inner wall sealing, like this when pulling or promoting push rod 201, utilize air pressure difference, blood is released in sleeve pipe 202 or is drawn into sleeve pipe 202.Sleeve pipe 202 front end arranges interface 204.Described sleeve pipe 202 tube wall arranges graduation mark 203, knows the blood or total plasma volume that extract or release easily.
Described bloodletting tube 3 main body is made up of a hollow sleeve 304, and hollow sleeve 304 can hold the blood extracted from patient, and hollow sleeve 304 arranges the handle 302 of protrusion topmost, handle 302 is arranged two or three connectors 301.Described hollow sleeve 304 surface arranges scale mark 303, can total amount on demand from the corresponding blood of patient's withdraw.
During use, move the slurry interface 204 of pipe 2 and be connected with the connector 301 of bloodletting tube 3, the blood in bloodletting tube 3 is extracted into and moves in slurry pipe 2, and then be connected moving the slurry interface 204 of pipe 2 with the entrance 101 of centrifuge tube 1, promote push rod 201 and blood is moved on in centrifuge tube 1.
Claims (4)
1. a method for rapid extraction rich platelet high-concentration blood plasma, is characterized in that comprising the following steps:
1) delivered to by the blood extracted in bloodletting tube (3), bloodletting tube first injects anticoagulant in (3), and the volume of anticoagulant and whole blood is 1:8-10; Described anticoagulant is made up of 10% sodium citrate and 90%EDTA;
2) with moving slurry pipe (2), blood is moved on in centrifuge tube (1) from bloodletting tube (3) again, then agglutinant is added at centrifuge tube (1), make it form gel, the volume of agglutinant and whole blood is 1:8-9, and described agglutinant is made up of 10% calcium chloride and 90% thrombin; Put centrifuge tube (1) into centrifuge again, with the centrifugal 4-6 minute of rotating speed 2500-3500rpm, blood is divided into 3 layers, bottom is the maximum erythrocyte of sedimentation coefficient, the superiors are supernatant and platelet-poor plasma, and both have skim by intersection, and this thin layer is platelet rich plasma layer;
3) then supernatant and platelet rich plasma layer is extracted, avoid extracting erythrocyte as far as possible, change centrifugal force recentrifuge, with the centrifugal 8-12 minute of rotating speed 1200-1800rpm, blood is divided into 2 layers, and upper strata is supernatant, and lower floor is platelet rich plasma layer, the supernatant of cumulative volume 3/4-4/5 in centrifuge tube (1) is removed, and remaining is rich platelet high-concentration blood plasma;
4) the rich platelet high-concentration blood plasma of acquisition is concentrated further, recentrifuge is separated, rotating speed is with the centrifugal 10-15 minute of 1000-1400rpm, and the upper strata of cumulative volume 1/4-1/5 in centrifuge tube (1) is removed, and remaining is concentrated rich platelet high-concentration blood plasma.
2. the method for a kind of rapid extraction rich platelet high-concentration blood plasma as claimed in claim 1, is characterized in that step 2) in: the rotating speed 2800-3000rpm of centrifuge, centrifugation time is 5 minutes.
3. the method for a kind of rapid extraction rich platelet high-concentration blood plasma as claimed in claim 1, it is characterized in that in step 3): the rotating speed 1400-1600rpm of centrifuge, centrifugation time is 9-10 minute.
4. a special equipment for rapid extraction rich platelet high-concentration blood plasma method according to claim 1, is characterized in that comprising centrifuge tube (1), moves slurry pipe (2), bloodletting tube (3);
Described centrifuge tube (1) comprises body (104), body (104) top is equipped with seal cover (102), the upper inlet porting (101) of seal cover (102), body (104) middle and upper part arranges thin neck (105), thin neck (105) outer wall of body (104) middle and upper part is equipped with safe wing (107), on the outer wall of body (104) bottom, socket arranges protective sleeve (108), body (104) wall body middle and lower part arranges and controls reference line (106), and wall body top arranges overall control line (103);
Described slurry pipe (2) that moves is made up of sleeve pipe (202), push rod (201) cooperation, push rod (201) front end is equipped with sealing ring, push rod (201) is slidably matched by sealing ring and sleeve pipe (202) inner wall sealing, sleeve pipe (202) front end arranges interface (204), and described sleeve pipe (202) tube wall arranges graduation mark (203);
Described bloodletting tube (3) main body is made up of a hollow sleeve (304), hollow sleeve (304) arranges the handle (302) of protrusion topmost, handle (302) is arranged two or three connectors (301), described hollow sleeve (304) surface arranges scale mark (303);
During use, move the slurry pipe interface (204) of (2) to be connected with the connector (301) of bloodletting tube (3), blood in bloodletting tube (3) is extracted into and moves in slurry pipe (2), and then be connected moving the slurry pipe interface (204) of (2) with the entrance (101) of centrifuge tube (1), promote push rod (201) and blood is moved on in centrifuge tube (1).
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| Publication number | Priority date | Publication date | Assignee | Title |
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| JP5896571B2 (en) * | 2013-10-15 | 2016-03-30 | キム ホンＫｉｍ Ｈｏｎｇ | Apparatus and method for extracting highly concentrated plasma from whole blood |
| CN103566629B (en) * | 2013-11-04 | 2015-05-06 | 常州尚泰医疗科技有限公司 | Method and device for separating and extracting platelet rich blood |
| CN104307208B (en) * | 2014-09-28 | 2016-08-24 | 浙江中医药大学 | A kind of hematoblastic method of enriching and purifying |
| CN104856914B (en) * | 2015-04-09 | 2017-10-20 | 广州军区广州总医院 | The application of platelet-poor plasma in the treatment of skin |
| CN106076448A (en) * | 2016-07-27 | 2016-11-09 | 上海凯戎医疗科技有限公司 | A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma |
| CN106582908B (en) * | 2016-11-30 | 2019-09-13 | 深圳市达科为生物工程有限公司 | A kind of platelet rich plasma fast separation device and method |
| CN108744608A (en) * | 2018-06-19 | 2018-11-06 | 中国人民解放军陆军军医大学第附属医院 | Itself a kind of improved platelet rich plasma preparation method |
| CN110664735A (en) * | 2019-10-16 | 2020-01-10 | 内江市第一人民医院 | Platelet-rich gel and preparation method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2721094Y (en) * | 2004-07-21 | 2005-08-31 | 张长青 | Centrifugal tube for preparing blood cell enriched plasma |
| CN102847571A (en) * | 2012-09-21 | 2013-01-02 | 北京上惠好生物技术有限公司 | Device of medical injector type centrifuge tube and projecting jacket |
| CN203169690U (en) * | 2013-04-01 | 2013-09-04 | 浙江康慈医疗科技有限公司 | Special equipment for quickly extracting platelet-rich and high-concentration plasma |
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