CN214439191U

CN214439191U - Device for preparing injection type autologous i-PRF

Info

Publication number: CN214439191U
Application number: CN202022755624.8U
Authority: CN
Inventors: 邓欣欣; 李罗浩; 李鉴墨; 严炎刘星; 佟真
Original assignee: Kangxi Biomedical Shenzhen Co ltd
Current assignee: Kangxi Biomedical Shenzhen Co ltd
Priority date: 2020-11-24
Filing date: 2020-11-24
Publication date: 2021-10-22
Anticipated expiration: 2030-11-24

Abstract

The utility model relates to a device for preparing injection type autologous i-PRF, which comprises a centrifuge tube with a barrel-shaped structure which can be placed in a centrifuge, wherein the mouth of the centrifuge tube is hermetically connected with a protective cover which can lead an injection needle to pass through; an axial opening is axially formed in the side wall of the centrifugal tube, and a pierceable membrane which is used for sealing the axial opening and can be pierced is hermetically connected to the axial opening; the side wall of the centrifugal tube is also provided with a sliding block which slides in the axial opening in a sliding way, and the sliding block is provided with a through hole corresponding to the axial opening. The utility model has the advantages of simple structure, convenient operation, reduced blood pollution's probability, made things convenient for the preparation of liquid i-PRF to draw.

Description

Device for preparing injection type autologous i-PRF

Technical Field

The utility model relates to the field of biomedicine, in particular to a device for preparing injection type autologous i-PRF.

Background

Autologous Platelet Rich Plasma (PRP) was first proposed in the 70 s of the 20 th century and began to be widely used in the 80 s of the 20 th century. To continue the advantages of PRP and to avoid the above disadvantages, due to the addition of an anticoagulant component that can cause immunological rejection and is not easy to handle, Choukroun, a second generation platelet concentrate prepared in france in 2001, called Platelet Rich Fibrin (PRF), is a fibrin clot rich in high concentration of platelets obtained by one centrifugation of autologous venous blood without the addition of anticoagulant. From the ultrastructure, it is found that the difference of the conformation of the fibrin network structure is the main structural feature for distinguishing the two structures, and the difference also has obvious difference in density and type. The density of fibrin is determined by the amount of fibrinogen that is the starting material, and the type of fibrin depends on the amount of thrombin and the rate of polymerization. In the preparation process of the traditional PRP, unpolymerized fibrin is dissolved in PPP and is directly discarded, so that the content of fibrinogen is greatly reduced when the subsequent thrombin is added for enzymatic coagulation, the density of a polymerized fibrin network structure is far lower than that of a physiological blood clot, the polymerization speed of fibrinogen is far higher than that of a physiological reaction due to the action of an exogenous additive and the high thrombin concentration, and the formed fibrin network is formed by polymerizing four molecular fibrinogen, is stiff and lacks elasticity, and is not beneficial to net cell factors and promoting cell migration. Therefore, the maturation of the fibrin network of PRF is better than that of PRP and closer to the physiological state. Meanwhile, the release modes of the cell factors of the PRF and the traditional PRP product are different, the PRP product activates the blood platelets in the PRP instantly due to the participation of the exogenous additive, and the cell factors released after the activation also have the cell factors with the function of accelerating the activation of the blood platelets, so the release of the cell factors is concentrated near the point of adding the exogenous additive, namely the early stage of the healing period after the use appears, the release at the later stage is less, and the release of the cell factors is not balanced and lasting. In the clinical application fields of cartilage repair, tendon repair, bone tissue repair and the like, the sustained release of the growth factors can more effectively promote the repair of tissues due to the long treatment and rehabilitation period, and the cell factors can be relatively and persistently released due to the structural advantage of PRF, so that the requirements of different clinical applications are met to a greater extent.

In recent years, there have been many studies demonstrating that PRF can promote soft tissue repair and regeneration, and PRF has also begun to be used as a supplement to tissue regeneration. However, the current devices are not conducive to the preparation and extraction of liquid PRF, the PRF is mainly in the form of fiber clumps, the PRF in this form is not conducive to the binding with other biomaterials, which limits its clinical use and also presents the problem of blood contamination during the preparation process.

SUMMERY OF THE UTILITY MODEL

Aiming at the existing defects, the utility model provides a device for preparing injection type autologous i-PRF.

The utility model provides a technical scheme that its technical problem adopted is: the device for preparing the injection type autologous i-PRF comprises a centrifuge tube with a barrel-shaped structure which can be placed in a centrifuge for centrifugation, wherein the tube opening of the centrifuge tube is hermetically connected with a protective cover through which an injection needle can pass; an axial opening is axially formed in the side wall of the centrifugal tube, and a pierceable membrane which is used for sealing the axial opening and can be pierced is hermetically connected to the axial opening; still the smooth slider that is equipped with in the internal-sliding of axial opening on the lateral wall of centrifuging tube, be provided with the through-hole that corresponds to axial opening on the slider.

Preferably, the protective cover is a cover with an insertion through hole in the center, and the diameter of the insertion through hole is matched with the pipe diameter of the injection needle.

Preferably, the protective cap is a protective membrane that can be pierced by an injection needle.

Preferably, the protective film and the pierceable film are integrally formed and hermetically connected to the side wall of the centrifuge tube.

Preferably, the pierceable membrane is hermetically connected to the end of the axial opening and is flush with the inner wall of the centrifuge tube, and the sliding block is arranged in the axial opening in a sliding manner and clings to the pierceable membrane.

Preferably, the slide block is further provided with an injection hole communicated with the through hole, and a multi-way valve is mounted in the through hole.

Preferably, the slider can be detachably connected with a hollow puncture piece which can be inserted into the through hole, and the puncture piece is hermetically connected with a collection container which can be communicated with the hollow part and can absorb liquid in the centrifugal tube.

The beneficial effects of the utility model reside in that:

1, the utility model has simple structure and convenient use, reduces the risk of blood contacting bacteria in use, and reduces the probability of blood pollution;

2, in the preparation process, except that the process of membrane puncture may bring in external bacteria, other processes blood does not contact bacteria;

3, the utility model can simply, quickly and effectively prepare the liquid i-PRF, thereby expanding the range of clinical application and solving the problem that the PRF prepared by the traditional device is in the form of fiber gel.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is an enlarged schematic structural diagram of a in fig. 1 according to an embodiment of the present invention;

FIG. 3 is a schematic view of the combination of a piercing member and a collection container according to an embodiment of the present invention;

part names and serial numbers in the figure: 1-centrifuge tube 10-axial opening 11-pierceable membrane 2-protective cover 20-insertion through hole 3-slide block 30-through hole 31-injection hole 4-piercing element 5-collection container.

Detailed Description

To clearly illustrate the objects, technical solutions and advantages of the embodiments of the present invention, the present invention will be further described in conjunction with the embodiments, which will be described in detail and fully hereinafter. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention. Furthermore, directional terms as referred to in the present disclosure, such as "upper", "lower", "front", "rear", "left", "right", "inner", "outer", etc., refer only to the direction of the attached drawings, and directional terms used are intended to better and more clearly illustrate and understand the present invention rather than to indicate or imply the orientation the present invention must have, and therefore should not be construed as limiting the present invention.

The embodiment of the utility model provides a, as shown in fig. 1-3, a device for preparing injection type autologous i-PRF, including can place centrifugal barrel-shaped structure's centrifuging tube 1 in centrifuge, and the one end of centrifuging tube 1 is open-ended other end and is closed, and the centre is hollow and is used for holding the holding cavity of the autologous blood of gathering, the mouth of pipe sealing connection of centrifuging tube 1 has the visor 2 that enables the syringe needle to pass, and mouth of pipe sealing connection visor 2 makes centrifuging tube 1 can not spill blood in the centrifugation, has also avoided the contact of blood and air, and in order to enable blood to be injected into centrifuging tube 1, visor 2 just needs to be passed by the syringe needle, in practical application, just uses syringe and syringe needle earlier to carry out autologous blood sampling, gathers blood to the syringe in, later removes the blood taking needle, changes into aseptic blood taking needle and is connected with the syringe, then the injection needle passes through the protective cover 2, and the collected non-anticoagulation venous blood is injected into the centrifuge tube 1 along the inner wall of the centrifuge tube 1, the collection amount of the blood is collected according to different conditions, and the collection amount is preferably 0-10 ml. At this time, for the protective cover 2, one mode is that the protective cover 2 is a cover with an insertion through hole 20 in the center, and the aperture of the insertion through hole 20 is matched with the tube diameter of the injection needle, so that the protective cover 2 is hermetically connected to the tube opening of the centrifuge tube 1, when blood is injected into the centrifuge tube 1, the injection needle is inserted into the insertion through hole 20 arranged in the center of the protective cover 2, and the head of the injection needle extends into the centrifuge tube 1, so that the blood can be conveniently injected into the centrifuge tube 1, and meanwhile, because the aperture of the insertion through hole 20 is matched with the tube diameter of the injection needle, the aperture is relatively small, and the blood centrifugation cannot be affected; the other mode is that the protective cover 2 is a protective film which can be pierced by the injection needle, the protective film is a rubber composite film, and the protective film can be hermetically connected with the opening of the centrifuge tube 1 in a thermoplastic packaging mode during production, so that the structure is simplified, the use is more facilitated, and the injection needle can be pierced through the protective film during use; meanwhile, the two modes can be combined for use, when the centrifugal tube is combined for use, a protective film is connected to the centrifugal tube 1 in a sealing mode, and then a cover with an insertion through hole 20 is installed on the protective film; the side wall of the centrifugal tube 1 is provided with an axial opening 10 along the axial direction, namely, the axial opening 10 extends from the tube opening of the centrifugal tube to the tube bottom, the axial opening 10 is hermetically connected with a pierceable membrane 11 which is used for sealing the axial opening 10 and can be pierced, the axial opening 10 is sealed by the pierceable membrane 11, so that the centrifugal tube 1 is still a tube body with a hollow barrel-shaped structure inside and can be used for containing blood, the pierceable membrane can be pierced to facilitate extraction of PRF after blood layering, the pierceable membrane 11 can also be a rubber composite membrane and is hermetically connected to the centrifugal tube 1 in a thermoplastic encapsulation mode, and under the condition that the protective cover 2 is a protective membrane, the protective membrane and the pierceable membrane 11 can be integrally formed and hermetically connected to the side wall of the centrifugal tube 1, so that the structure of the product is further simplified, and the cost can be better reduced; the side wall of the centrifuge tube 1 is further provided with a sliding block 3 which slides in the axial opening 10 in a sliding manner, the sliding block 3 is provided with a through hole 30 corresponding to the axial opening 10, the sliding block 3 can be positioned at different heights of the centrifuge tube 1 due to the sliding in the axial opening 10, the sliding is realized through a sliding rail and a sliding groove which are matched with each other, the sliding rail and the sliding groove are correspondingly arranged on the centrifuge tube 1 and the sliding block 3, the centrifugal tube 1 is provided with the sliding groove, the sliding rail is arranged on the sliding block 3, the reverse is realized, the sliding rail is arranged on the centrifuge tube 1, and the sliding groove is arranged on the sliding block 3; the through hole 30 corresponds to the axial opening 10, facilitating the piercing of the pierceable membrane 11 in use. The device has simple structure and convenient use, can effectively reduce the risk of blood contacting bacteria in use, and also reduces the probability of blood pollution.

Further improvement, but puncture membrane 11 sealing connection is held flat at axial opening 10's tip and with centrifuge tube 1's inner wall mutually, can puncture membrane 11 and be set up the one side that is located centrifuge tube 1 inner wall at axial opening 10, and be flat just means can not have the seam between the inner wall of puncture membrane 11 and centrifuge tube 1 with centrifuge tube 1 inner wall is lasting, just can not influence the centrifugation to blood yet, separation PRF layer and red blood cell layer that can be better, slider 3 is hugged closely and can be punctured membrane 11 and establish in axial opening 10 cunning, just makes the part that is used for the puncture reduce the chance that contacts with external environment at the in-process that punctures puncture membrane 11, has reduced the contaminated probability of blood.

In a further improvement, as shown in fig. 1 and 2, the slider 3 is further provided with an injection hole 31 communicated with the through hole 30, and a multi-way valve (not shown) is installed in the through hole 30, so that other biomaterials, such as collagen, hyaluronic acid, etc., can be injected through the injection hole 31, and then the collected PRF and other biomaterials can be conveniently mixed together by controlling the multi-way valve.

In a further improvement, the slide block 3 can be detachably connected with a puncture piece 4 which can be inserted into the through hole 30 and is hollow inside, as shown in fig. 3, the puncture piece 4 is hermetically connected with a collection container 5 which can be communicated with the hollow part and can suck the liquid in the centrifuge tube 1; therefore, after blood is centrifuged by the centrifuge tube 1, the liquid PRF separated in the centrifuge tube 1 can conveniently penetrate through the through hole 30 to pierce the pierceable membrane through the piercing element 4, so that the liquid PRF can flow out through the hollow part inside the piercing element 4, the piercing element 4 can be a conventional piercing needle or a piercing structure with a piercing head part, the tail part is provided with a connecting structure connected with the collecting container 5, such as a clamping structure, at the moment, the collecting container 5 can be a cylindrical structure, one end of the collecting container is connected and communicated with the piercing element 4, the other end of the collecting container is provided with a sliding part which slides in the piercing element 4, such as an injector which is conventionally used, the piercing element 4 can also be a piercing structure with a piercing head part and a tail part, the collecting container 5 is internally vacuum and has a container which can be pierced by the piercing element 4, and the piercing element 4 can pierce the blood due to the pressure difference between the inside and the outside of the collecting container 5, the centrifuged PRF in the centrifuge tube 1 can be sucked into the collection container 5.

When the device is used for preparing the i-PRF, firstly, a blood taking needle is selected to be connected with an injector, blood is collected into the injector, then the blood taking needle is removed and replaced by a sterile injection needle to be connected with the injector, and finally the blood is injected into a centrifuge tube through the injection needle passing through a protective cover on the centrifuge tube; then placing the centrifuge tube into a centrifuge, balancing, then carrying out low-speed centrifugation to divide the blood into a PRF layer and a red blood cell layer, after injecting the blood into the centrifuge tube, immediately placing the centrifuge tube into the centrifuge for low-speed centrifugation, wherein the low-speed centrifugation is carried out under the conditions that the room temperature is 20-25 ℃, the centrifugal force is 40-60g, and the centrifugation time is not more than 5min, taking out the centrifuge tube after the centrifugation is finished, sliding a slide block to enable a through hole on the slide block to be aligned with the junction of the PRF layer and the red blood cell layer or the position 1-1.5mm below the junction, enabling any height position of the centrifuge tube stopped by the slide block to conveniently puncture a pierceable membrane by using a puncture needle to suck the PRF, extracting the PRF layer to a collection container, and finally shaking up the PRF in the collection container to obtain i-PRF, wherein the volume of the extracted i-PRF is 1/10-1/2 of the blood collection volume, the obtained i-PRF can be used as a cartilage repair material and is prepared at any time.

It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are considered to be within the scope of the invention as defined by the following claims.

Claims

1. An apparatus for preparing an injectable autologous i-PRF, characterized in that: the centrifuge tube comprises a centrifuge tube with a barrel-shaped structure which can be placed in a centrifuge for centrifugation, and a tube opening of the centrifuge tube is hermetically connected with a protective cover through which an injection needle can pass; an axial opening is axially formed in the side wall of the centrifugal tube, and a pierceable membrane which is used for sealing the axial opening and can be pierced is hermetically connected to the axial opening; still the smooth slider that is equipped with in the internal-sliding of axial opening on the lateral wall of centrifuging tube, be provided with the through-hole that corresponds to axial opening on the slider.

2. The apparatus for preparing injectable autologous i-PRF according to claim 1, wherein the protective cap is a cap having an insertion through hole at the center, and the diameter of the insertion through hole is matched with the diameter of the injection needle.

3. The apparatus for preparing an injectable autologous i-PRF according to claim 1, wherein said protective cap is a protective film that can be punctured by an injection needle.

4. The apparatus for preparing an injectable autologous i-PRF according to claim 3, wherein the protective membrane and the pierceable membrane are integrally formed and hermetically connected to the side wall of the centrifuge tube.

5. The apparatus for preparing an injectable autologous i-PRF according to claim 1, wherein the pierceable membrane is sealingly attached to the end of the axial opening and is flush with the inner wall of the centrifuge tube, and the sliding block is slidably disposed in the axial opening along the pierceable membrane.

6. The device for preparing the injection-type autologous i-PRF according to claim 1, wherein the slide block is further provided with an injection hole communicated with the through hole, and a multi-way valve is arranged in the through hole.

7. The device for preparing the injection-type autologous i-PRF according to claim 1, wherein the slider is further detachably connected with a hollow puncturing member which can be inserted into the through hole, and the puncturing member is hermetically connected with a collecting container which can be communicated with the hollow part and can suck the liquid in the centrifugal tube.