CN103566629B - Method and device for separating and extracting platelet rich blood - Google Patents
Method and device for separating and extracting platelet rich blood Download PDFInfo
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- CN103566629B CN103566629B CN201310539497.XA CN201310539497A CN103566629B CN 103566629 B CN103566629 B CN 103566629B CN 201310539497 A CN201310539497 A CN 201310539497A CN 103566629 B CN103566629 B CN 103566629B
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Abstract
The invention discloses a method and a device for separating and extracting a platelet rich in blood. Venous blood is extracted by a medical injector, is injected into a device, and is separated by utilizing a single device, and the platelet cell is extracted for later use, wherein the device comprises a PRP (platelet rich plasma) storage chamber, a plasma storage chamber, a locking lever spinner and an erythrocyte storage chamber from top to bottom. The method and the device for separating and extracting the platelet rich in the blood overcome the defects that a traditional platelet-rich blood separating and extracting operation uses more tools, is not convenient to operate and easily causes pollution, are simple in structure and convenient to operate, can complete the whole separation process of blood in only one device, and have a remarkable effect.
Description
Technical field
The present invention relates to technical field of medical instruments, especially a kind of blood rich platelet separating and extracting process and device.
Background technology
Medically to use a whole set of a lot of device, test tube, syringe etc. in the process doing blood rich platelet separation and Extraction at present, also be operationally quite complicated, will through multiple injection, centrifugal, extraction, very inconvenient, and easily cause pollution, therefore this kind of Method and kit for all will improve, and makes the simplification of whole operating procedure, pollution-freeization.
Summary of the invention
The technical problem to be solved in the present invention is: in order to solve existing blood rich platelet separation and Extraction operation Problems existing in above-mentioned background technology, provide a kind of blood rich platelet separating and extracting process and device of improvement.
The technical solution adopted in the present invention is: a kind of blood rich platelet separating and extracting process, has separating extraction device, and the structure of this separating extraction device is:
Comprise PRP locker room from top to bottom, blood plasma locker room, PRP/ red blood cell securing rod swing handle and red blood cell locker room, described blood plasma locker room is threaded with PRP locker room and PRP/ red blood cell securing rod swing handle respectively, and threaded connection place is provided with silica gel sealing ring, PRP/ red blood cell securing rod swing handle and the fixed seal connection of red blood cell locker room, junction is communicated with by blood plasma entrance hole, the serum entrance hole of a taper is also provided with between PRP locker room and blood plasma locker room, a PRP securing rod is also provided with in described blood plasma locker room, this PRP securing rod is fixedly connected with blood plasma locker room and follows it and moves, and this PRP securing rod runs through the space between serum entrance hole and blood plasma entrance hole from top to bottom successively, the two ends caliber size of described PRP securing rod coincide with the aperture size of described serum entrance hole and blood plasma entrance hole respectively, the space up and down of isolation corresponding entrance hole can be closed when PRP securing rod is entered in corresponding entrance hole, described PRP locker room and red blood cell locker room have all been threaded connection lid, threaded connection place is sealed by silica gel sealing ring,
In the lid of described PRP locker room, sealing is provided with silica gel plug and is coated with protection foil film on silica gel plug;
The sidewall of described blood plasma locker room is provided with two symmetrical holes, one is blood hand-hole, another is filter ventilation hole, described blood hand-hole is injected with a plug by double-colored pair of material, filter ventilation hole is pressed with a ventilative resistance mycoderm by silica gel pressure ring, and is provided with a dismountable Capsule.
Concrete steps are:
A, use injector for medical purpose venous blood samples, this device is upright, and opened by the Capsule in filter ventilation hole, and pierce through silica gel plug from blood hand-hole and inject venous blood in blood plasma locker room, blood flows into red blood cell locker room by blood plasma locker room;
B, the ACD that simultaneously gets ?A anti-coagulants, anti-coagulants and blood ratios 1:10, injected by blood hand-hole, closes filter ventilation hole;
C, this device is put in centrifuge, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, the lid of red blood cell locker room is regulated to be regulated above the closed place of blood plasma entrance hole by top serum, in order to ensure that red blood cell does not infiltrate serum, stay the serum liquid level of 2mm ?3mm height in the below at the closed place of blood plasma entrance hole, rotation PRP/ red blood cell securing rod swing handle makes PRP securing rod enter blood plasma entrance hole and makes its closed isolation;
D, this device to be inverted, the serum layer isolated is entered PRP locker room by the hollow hole of blood plasma locker room;
E, this device is put on centrifuge centrifugal, blood will layering, bottom is platelet cell, top is impurity layer, regulate the lid of PRP locker room, on the serum entrance hole impurity layer on top being adjusted to PRP locker room, in order to ensure that free from admixture is referred and synthesized, simultaneously by 2mm ?the platelet cell layer of 3mm liquid level be also modulated on serum entrance hole, then PRP securing rod is adjusted to taper hole and closes and cut off;
F, tear the protection foil film of the tops of PRP locker room 1 off, injector for medical purpose is thrust silica gel plug to PRP locker room, extract platelet rich plasma for subsequent use.
The device that above-mentioned blood rich platelet separating and extracting process is used: comprise PRP locker room from top to bottom, blood plasma locker room, PRP/ red blood cell securing rod swing handle and red blood cell locker room, described blood plasma locker room is threaded with PRP locker room and PRP/ red blood cell securing rod swing handle respectively, and threaded connection place is provided with silica gel sealing ring, PRP/ red blood cell securing rod swing handle and the fixed seal connection of red blood cell locker room, junction is communicated with by blood plasma entrance hole, the serum entrance hole of a taper is also provided with between PRP locker room and blood plasma locker room, a PRP securing rod is also provided with in described blood plasma locker room, this PRP securing rod is fixedly connected with blood plasma locker room and follows it and moves, and this PRP securing rod runs through the space between serum entrance hole and blood plasma entrance hole from top to bottom successively, the two ends caliber size of described PRP securing rod coincide with the aperture size of described serum entrance hole and blood plasma entrance hole respectively, the space up and down of isolation corresponding entrance hole can be closed when PRP securing rod is entered in corresponding entrance hole, described PRP locker room and red blood cell locker room have all been threaded connection lid, threaded connection place is sealed by silica gel sealing ring,
In the lid of described PRP locker room, sealing is provided with silica gel plug and is coated with protection foil film on silica gel plug;
The sidewall of described blood plasma locker room is provided with two symmetrical holes, one is blood hand-hole, another is filter ventilation hole, described blood hand-hole is injected with a plug by double-colored pair of material, filter ventilation hole is pressed with a ventilative resistance mycoderm by silica gel pressure ring, and is provided with a dismountable Capsule.
The invention has the beneficial effects as follows: a kind of blood rich platelet separating and extracting process of the present invention and device overcome many, the inconvenient operation of use instrument that the operation of conventional blood rich platelet separation and Extraction exists, the defect easily causing pollution, structure is simple, easy to operate, only need can complete the whole separation process of blood in a device, there is significant progress.
Accompanying drawing explanation
Below in conjunction with drawings and Examples, the present invention is further described.
Fig. 1 is the structural representation of the preferred embodiments of the present invention;
Tu Zhong 1.PRP locker room, 1-1. serum entrance hole, 2. blood plasma locker room, 2-1.PRP securing rod, 3.PRP/ red blood cell securing rod swing handle, 3-1. blood plasma entrance hole, 4. red blood cell locker room, 5. silica gel sealing ring, 6. silica gel plug, 7. blood hand-hole, 8. filter ventilation hole.
Detailed description of the invention
The present invention is further detailed explanation with preferred embodiment by reference to the accompanying drawings now.These accompanying drawings are the schematic diagram of simplification, only basic structure of the present invention are described in a schematic way, and therefore it only shows the formation relevant with the present invention.
The embodiment of a kind of blood rich platelet separating and extracting process of the present invention as shown in Figure 1 and device,
The concrete steps of described method are:
A, use 50ml injector for medical purpose venous blood samples, this device is upright, and opened by the Capsule in filter ventilation hole 8, and pierce through silica gel plug injection venous blood in blood plasma locker room 2 from blood hand-hole 7, blood flows into red blood cell locker room 4 by blood plasma locker room 2;
B, get simultaneously 5ml ACD ?A anti-coagulants (anti-coagulants and blood ratios 1:10), injected by blood hand-hole 7, close filter ventilation hole 8;
C, this device to be put on centrifuge with the centrifugal force 10min of 200g, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, regulate the lid of red blood cell locker room 4 top serum is regulated blood plasma entrance hole 3 ?1 closed place above, in order to ensure that red blood cell does not infiltrate serum, stay serum liquid level about 3mm height in the below at the closed place of blood plasma entrance hole 3 ?1, rotate PRP/ red blood cell securing rod swing handle 3 and make PRP securing rod 2 ?1 enter blood plasma entrance hole 3 ?1 to make it close isolation;
D, this device to be inverted, the serum layer isolated is entered PRP locker room 1 by the hollow hole of blood plasma locker room 2;
E, this device to be put on centrifuge with the centrifugal force 10min of 200g, blood will layering, bottom is platelet cell, top is impurity layer, regulate the lid of PRP locker room 1, the impurity layer on top is adjusted to PRP locker room 1 serum entrance hole 1 ?on 1, in order to ensure that free from admixture is referred and synthesized, the platelet cell layer of 2mm liquid level is also modulated to simultaneously serum entrance hole 1 ?on 1, then by PRP securing rod 2 ?1 be adjusted to taper hole and close and cut off;
F, tear the protection foil film of the tops of PRP locker room 1 off, injector for medical purpose is thrust silica gel plug 6 to PRP locker room 1, extract platelet rich plasma for subsequent use.
The device that above-mentioned blood rich platelet separating and extracting process is used: comprise PRP locker room 1 from top to bottom, blood plasma locker room 2, PRP/ red blood cell securing rod swing handle 3 and red blood cell locker room 4, described blood plasma locker room 2 is threaded with PRP locker room 1 and PRP/ red blood cell securing rod swing handle 3 respectively, and threaded connection place is provided with silica gel sealing ring 5, PRP/ red blood cell securing rod swing handle 3 and red blood cell locker room 4 fixed seal connection, junction is communicated with by blood plasma entrance hole 3-1, the serum entrance hole 1-1 of a taper is also provided with between PRP locker room 1 and blood plasma locker room 2, in described blood plasma locker room, 2 are also provided with a PRP securing rod 2-1, this PRP securing rod 2-1 is fixedly connected with blood plasma locker room 2 and follows it and moves, and this PRP securing rod 2-1 runs through the space between serum entrance hole 1-1 and blood plasma entrance hole 3-1 from top to bottom successively, the two ends caliber size of described PRP securing rod 2-1 coincide with the aperture size of described serum entrance hole 1-1 and blood plasma entrance hole 3-1 respectively, the space up and down of isolation corresponding entrance hole can be closed when PRP securing rod 2-1 is entered in corresponding entrance hole, described PRP locker room 1 and red blood cell locker room 4 have all been threaded connection lid, threaded connection place is sealed by silica gel sealing ring.
Further, the interior sealing of the lid of PRP locker room 1 of the present invention is provided with silica gel plug 6 and is coated with protection foil film on silica gel plug 6.
Further, the sidewall of blood plasma locker room 2 of the present invention is provided with two symmetrical holes, one is blood hand-hole 7, another is filter ventilation hole 8, described blood hand-hole 7 is injected with a plug by double-colored pair of material, filter ventilation hole 8 is pressed with a ventilative resistance mycoderm by silica gel pressure ring, and is provided with a dismountable Capsule.
The just the specific embodiment of the present invention described in above description, various illustrating is not construed as limiting flesh and blood of the present invention, person of an ordinary skill in the technical field after having read description can to before described detailed description of the invention make an amendment or be out of shape, and do not deviate from essence of an invention and scope.
Claims (2)
1. a blood rich platelet separating and extracting process, is characterized in that: have separating extraction device, and the structure of this separating extraction device is:
Comprise PRP locker room (1) from top to bottom, blood plasma locker room (2), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4), described blood plasma locker room (2) is threaded with PRP locker room (1) and PRP/ red blood cell securing rod swing handle (3) respectively, and threaded connection place is provided with silica gel sealing ring (5), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4) fixed seal connection, junction is communicated with by blood plasma entrance hole (3-1), the serum entrance hole (1-1) of a taper is also provided with between PRP locker room (1) and blood plasma locker room (2), in described blood plasma locker room, (2) are also provided with a PRP securing rod (2-1), this PRP securing rod (2-1) is fixedly connected with blood plasma locker room (2) and follows it and moves, and this PRP securing rod (2-1) runs through the space between serum entrance hole (1-1) and blood plasma entrance hole (3-1) from top to bottom successively, the two ends caliber size of described PRP securing rod (2-1) coincide with the aperture size of described serum entrance hole (1-1) and blood plasma entrance hole (3-1) respectively, the space up and down of isolation corresponding entrance hole can be closed when PRP securing rod (2-1) is entered in corresponding entrance hole, described PRP locker room (1) and red blood cell locker room (4) have all been threaded connection lid, threaded connection place is sealed by silica gel sealing ring,
In the lid of described PRP locker room (1), sealing is provided with silica gel plug (6) and is coated with protection foil film on silica gel plug (6);
The sidewall of described blood plasma locker room (2) is provided with two symmetrical holes; one is blood hand-hole (7); another is filter ventilation hole (8); described blood hand-hole (7) is injected with a plug by double-colored pair of material; filter ventilation hole (8) is pressed with a ventilative resistance mycoderm by silica gel pressure ring, and is provided with a dismountable Capsule;
Concrete steps are:
A, use injector for medical purpose venous blood samples, this device is upright, the Capsule of filter ventilation hole (8) is opened, and piercing through silica gel plug injection venous blood in blood plasma locker room (2) from blood hand-hole (7), blood flows into red blood cell locker room (4) by blood plasma locker room (2);
B, the ACD that simultaneously gets ?A anti-coagulants, anti-coagulants and blood ratios 1:10, injected by blood hand-hole (7), closes filter ventilation hole (8);
C, this device is put in centrifuge, blood will layering, lower floor's liquid level is red blood cell, top liquid level is serum, the lid of red blood cell locker room (4) is regulated to regulate above the closed place of blood plasma entrance hole (3 ?1) by top serum, in order to ensure that red blood cell does not infiltrate serum, stay the serum liquid level of 2mm ?3mm height in the below at the closed place of blood plasma entrance hole (3 ?1), rotation PRP/ red blood cell securing rod swing handle (3) makes PRP securing rod (2 ?1) enter blood plasma entrance hole (3 ?1) and makes its closed isolation;
D, this device to be inverted, the serum layer isolated is entered PRP locker room (1) by the hollow hole of blood plasma locker room (2);
E, this device is put on centrifuge centrifugal, blood will layering, bottom is platelet cell, top is impurity layer, regulate the lid of PRP locker room (1), on the serum entrance hole (1 ?the 1) impurity layer on top being adjusted to PRP locker room (1), in order to ensure that free from admixture is referred and synthesized, simultaneously by 2mm ?the platelet cell layer of 3mm liquid level be also modulated on serum entrance hole (1 ?1), then PRP securing rod (2 ?1) is adjusted to taper hole and closes and cut off;
F, tear the protection foil film of the tops of PRP locker room 1 off, injector for medical purpose is thrust silica gel plug (6) to PRP locker room (1), extract platelet rich plasma for subsequent use.
2. a blood rich platelet separating extraction device, it is characterized in that: comprise PRP locker room (1) from top to bottom, blood plasma locker room (2), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4), described blood plasma locker room (2) is threaded with PRP locker room (1) and PRP/ red blood cell securing rod swing handle (3) respectively, and threaded connection place is provided with silica gel sealing ring (5), PRP/ red blood cell securing rod swing handle (3) and red blood cell locker room (4) fixed seal connection, junction is communicated with by blood plasma entrance hole (3-1), the serum entrance hole (1-1) of a taper is also provided with between PRP locker room (1) and blood plasma locker room (2), in described blood plasma locker room, (2) are also provided with a PRP securing rod (2-1), this PRP securing rod (2-1) is fixedly connected with blood plasma locker room (2) and follows it and moves, and this PRP securing rod (2-1) runs through the space between serum entrance hole (1-1) and blood plasma entrance hole (3-1) from top to bottom successively, the two ends caliber size of described PRP securing rod (2-1) coincide with the aperture size of described serum entrance hole (1-1) and blood plasma entrance hole (3-1) respectively, the space up and down of isolation corresponding entrance hole can be closed when PRP securing rod (2-1) is entered in corresponding entrance hole, described PRP locker room (1) and red blood cell locker room (4) have all been threaded connection lid, threaded connection place is sealed by silica gel sealing ring,
In the lid of described PRP locker room (1), sealing is provided with silica gel plug (6) and is coated with protection foil film on silica gel plug (6);
The sidewall of described blood plasma locker room (2) is provided with two symmetrical holes, one is blood hand-hole (7), another is filter ventilation hole (8), described blood hand-hole (7) is injected with a plug by double-colored pair of material, filter ventilation hole (8) is pressed with a ventilative resistance mycoderm by silica gel pressure ring, and is provided with a dismountable Capsule.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310539497.XA CN103566629B (en) | 2013-11-04 | 2013-11-04 | Method and device for separating and extracting platelet rich blood |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310539497.XA CN103566629B (en) | 2013-11-04 | 2013-11-04 | Method and device for separating and extracting platelet rich blood |
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| CN103566629A CN103566629A (en) | 2014-02-12 |
| CN103566629B true CN103566629B (en) | 2015-05-06 |
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| CN201310539497.XA Expired - Fee Related CN103566629B (en) | 2013-11-04 | 2013-11-04 | Method and device for separating and extracting platelet rich blood |
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Families Citing this family (14)
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| WO2016195126A1 (en) * | 2015-06-01 | 2016-12-08 | 이은혜 | Blood separation and extraction apparatus for extracting platelet-rich growth factor |
| CN105148569B (en) * | 2015-07-02 | 2017-03-01 | 周建明 | A kind of integration autologous platelet rich gel preparation suit |
| CN106582908B (en) * | 2016-11-30 | 2019-09-13 | 深圳市达科为生物工程有限公司 | A kind of platelet rich plasma fast separation device and method |
| KR101979382B1 (en) * | 2018-11-30 | 2019-05-17 | (주)레보메드 | Kit for Separating and Concentrating a Body Fluid Cell |
| CN110252525A (en) * | 2019-04-22 | 2019-09-20 | 福州比华利美容医院有限公司 | Blood ingredient separator tool |
| CN110639712A (en) * | 2019-10-11 | 2020-01-03 | 武汉济源高科技有限公司 | Autologous platelet-rich plasma separation preparation device |
| CN112058174B (en) * | 2020-09-24 | 2022-05-13 | 北京大学第三医院（北京大学第三临床医学院） | Platelet-rich plasma (PRP) spray gun device |
| CN114452684B (en) * | 2020-11-10 | 2024-10-11 | 菲尔德森(江苏)生物技术有限公司 | Platelet-rich plasma extraction and separation set and extraction method |
| CN113546450B (en) * | 2021-07-27 | 2022-06-28 | 伯格曼(江苏)医疗科技有限公司 | Multipurpose platelet and plasma preparation device |
| CN114939345A (en) * | 2022-06-21 | 2022-08-26 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and operation method thereof |
| CN114870436B (en) * | 2022-06-21 | 2024-08-06 | 浙江保尔曼生物科技有限公司 | Platelet-rich plasma preparation device and preparation method thereof |
| CN115475416B (en) * | 2022-09-13 | 2024-05-31 | 山东同齐医学科技有限公司 | Platelet-rich plasma preparation mechanism |
| CN115445683B (en) * | 2022-09-24 | 2023-08-22 | 北京市美嘉亿盛医疗器械股份有限公司 | PRP test tube |
| CN117126716B (en) * | 2023-09-06 | 2024-04-05 | 浏阳市三力医用科技发展有限公司 | Disposable special centrifuge tube for platelet-rich plasma and accessory thereof |
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| JP5105925B2 (en) * | 2007-03-26 | 2012-12-26 | 京セラメディカル株式会社 | Centrifugal device |
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| CN101528243A (en) * | 2006-10-27 | 2009-09-09 | 尼普洛株式会社 | Platelet-rich plasma separator and platelet-rich plasma separation method |
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