CN206706092U - A kind of centrifuge tube - Google Patents
A kind of centrifuge tube Download PDFInfo
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- CN206706092U CN206706092U CN201720514623.XU CN201720514623U CN206706092U CN 206706092 U CN206706092 U CN 206706092U CN 201720514623 U CN201720514623 U CN 201720514623U CN 206706092 U CN206706092 U CN 206706092U
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- centrifuge tube
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Abstract
It the utility model is related to a kind of centrifuge tube, particularly a kind of centrifuge tube for the separation of human peripheral blood composition.The centrifuge tube includes lid, elastic sealed body, centrifuge tube body, upper chamber, middle chamber, lower chambers and lower chambers bottom surface.The utility model is good to different cell layered effects, is easy to extract cell, and simple in construction, is more convenient for manufacturing.
Description
Technical field
The utility model is on a kind of centrifuge tube, particularly a kind of centrifuge tube for human peripheral blood.
Background technology
In biology, science of heredity and medical science, most basic operation during the separation of material.At present, in medical domain,
Research to stem cell is increasingly gradually deep.In order to obtain specific cell, often cell is separated.For example obtain adult
Main three kinds of methods of stem cell：Adult stem cell is obtained by marrow, adult stem cell is obtained and by outer by Cord blood
All blood obtains adult stem cell.In these methods, each composition of blood is separated by centrifugation first（Red blood cell is white thin
Born of the same parents, blood platelet and blood plasma）, then separate stem cell（Monocyte）.Conventional operating process is to remove centrifuge tube lid, is added
Enter the peripheral blood extracted, cover centrifuge tube lid, centrifuge tube is then placed in a centrifuge centrifugation.Generally, red blood cell is in blood
Proportion in liquid all the components is maximum, therefore is often centrifuging bottom of the tube after centrifuging, and red blood cell accounts for people's whole blood 47% or so.Position
What it is in red blood cell upper strata is leucocyte and blood platelet, and it accounts for 1% of whole blood or so, and wherein also includes monocyte（Peripheral blood
Stem cell）.Blood plasma and fibrin etc. are located at leucocyte and hematoblastic top.The centrifuge tube centrifuged is taken out from centrifuge
Afterwards, plug need to be again turned on, and is layered according to each material composition because of different specific weight come target needed for drawing.
In such operating process, exist open and close plug the step of, can make the foreign matter in air enter from
In heart pipe, there is a possibility that to pollute sample.Bored in addition, conventional centrifuge tube is upper middle part for a cylinder, bottom for class
Shape.Using this centrifuge tube, if the target after centrifugation is to be deposited in bottom, more convenient taking-up.If but required target be located at from
In the middle part of heart pipe or higher position, the larger diameter of centrifuge tube will be unfavorable for being precisely separated required target.
In consideration of it, it is necessary to provide a kind of centrifuge tube, to solve the above problems.
The content of the invention
The purpose of this utility model is to provide a kind of centrifuge tube, can conveniently and efficiently separate, extract in blood respectively into
Point.
To realize above-mentioned target, the utility model provides a kind of centrifuge tube, and the centrifuge tube includes lid, elastic sealed body,
Centrifuge tube body, upper chamber, middle chamber, lower chambers and lower chambers bottom surface.Wherein, the elastic sealed body is by rubber, silicones,
PVC, polyethylene, one or more materials are made in polypropylene.The lower chambers bottom surface is flexible, by rubber, silicones,
PVC, polyethylene, one or more materials are made in polypropylene.The upper chamber possesses the diameter same with lower chambers, in described
The half of a diameter of upper chamber of chamber.The lower chambers account for centrifuge tube cumulative volume about 40%, and the middle chamber accounts for centrifuge tube totality
Product about 10%.
The utility model has the advantages that.
Peripheral blood has been extracted in syringe, elastic sealed body can be inserted directly into and enter centrifuge tube, peripheral blood is discharged, then take out
Go out syringe.Whole process centrifuge tube internal objects do not contact extraneous air, can effectively avoid extraneous air to sample in centrifuge tube
This potential pollution.
In addition, because middle chamber diameter is only the half of upper and lower chamber, positioned at middle chamber into layering under equal volume,
There to be bigger height, be easy to extraction positioned at the composition of middle chamber.
Brief description of the drawings
Fig. 1 is the profile of centrifuge tube of the present utility model.
Fig. 2 is the schematic diagram of embodiment 1 of the present utility model.
In figure, 1, centrifuge tube lid；2nd, elastic sealed body；3rd, upper chamber；4th, centrifuge tube body；5th, middle chamber；6th, cavity of resorption
Room；7th, lower chambers bottom surface.
Embodiment
Below by way of specific embodiment, the utility model is expanded on further.
A kind of centrifuge tube, the centrifuge tube include lid 1, elastic sealed body 2, centrifuge tube body 4, upper chamber 3, middle chamber 5,
Lower chambers 6 and lower chambers bottom surface 7.Wherein, the elastic sealed body 2 is by rubber, silicones, PVC, polyethylene, one in polypropylene
Kind or multiple material are made.The lower chambers bottom surface 7 is flexible, by rubber, silicones, and PVC, polyethylene, one in polypropylene
Kind or multiple material are made.The upper chamber 3 possesses the diameter same with lower chambers 6, a diameter of upper chamber of the middle chamber 5
3 half.The lower chambers 6 account for centrifuge tube cumulative volume about 40%, and the middle chamber 5 accounts for centrifuge tube cumulative volume about 10%.
Embodiment 1：Appropriate Percoll solution is drawn using syringe, insertion elastic sealed body 2 enters in centrifuge tube,
Discharge Percoll solution.Then the syringe insertion elastic sealed body 2 for having extracted peripheral blood is entered in centrifuge tube, injection is outer
All blood, extract syringe out, centrifuged in centrifuge is told.After centrifugation, mixed liquor will appear from being layered.Such as Fig. 2, distinguish from bottom to top
It is red blood cell and granulocyte part, the part rich in lymphocyte, part and dead cell part rich in monocyte.
Claims (7)
1. a kind of centrifuge tube, it is characterised in that the centrifuge tube includes lid（1）, elastic sealed body（2）, centrifuge tube body（4）,
Upper chamber（3）, middle chamber（5）, lower chambers（6）With lower chambers bottom surface（7）.
2. centrifuge tube according to claim 1, it is characterised in that：The elastic sealed body（2）By rubber, silicones,
PVC, polyethylene, one or more materials are made in polypropylene.
3. centrifuge tube according to claim 1, it is characterised in that：The lower chambers bottom surface（7）It is flexible, by rubber,
Silicones, PVC, polyethylene, one or more materials are made in polypropylene.
4. centrifuge tube according to claim 1, it is characterised in that：The middle chamber（5）A diameter of upper chamber（3）One
Half.
5. centrifuge tube according to claim 1, it is characterised in that：The upper chamber（3）Possess and lower chambers（6）Same
Diameter.
6. centrifuge tube according to claim 1, it is characterised in that：The lower chambers（6）Account for centrifuge tube cumulative volume about 40%.
7. centrifuge tube according to claim 1, it is characterised in that：The middle chamber（5）Account for centrifuge tube cumulative volume about 10%.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720514623.XU CN206706092U (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720514623.XU CN206706092U (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206706092U true CN206706092U (en) | 2017-12-05 |
Family
ID=60465883
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720514623.XU Active CN206706092U (en) | 2017-05-10 | 2017-05-10 | A kind of centrifuge tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206706092U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057899A (en) * | 2019-04-14 | 2019-07-26 | 北京工业大学 | The system and method for anaerobic ammonium oxidation sludge extracellular polymeric molecular structure characterization |
-
2017
- 2017-05-10 CN CN201720514623.XU patent/CN206706092U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110057899A (en) * | 2019-04-14 | 2019-07-26 | 北京工业大学 | The system and method for anaerobic ammonium oxidation sludge extracellular polymeric molecular structure characterization |
| CN110057899B (en) * | 2019-04-14 | 2022-01-28 | 北京工业大学 | System and method for characterizing molecular structure of extracellular polymer of anaerobic ammonia oxidation sludge |
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