CN210384938U - Platelet-rich plasma collecting and separating container - Google Patents

Platelet-rich plasma collecting and separating container Download PDF

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Publication number
CN210384938U
CN210384938U CN201920810831.3U CN201920810831U CN210384938U CN 210384938 U CN210384938 U CN 210384938U CN 201920810831 U CN201920810831 U CN 201920810831U CN 210384938 U CN210384938 U CN 210384938U
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tube
test tube
separator tube
plug
platelet
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朱德新
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Abstract

The utility model relates to a blood collection separation technical field is a separating vessel is gathered to rich platelet plasma particularly, including test tube and plug, its characterized in that: the intraductal separator tube that is equipped with of test, separator tube lining in the inside of test tube, for round bottom hollow container, divide upper and middle and lower three-section, the upper end opening, the semicircular seal that goes to the bottom, the upper and lower lumen diameter of separator tube is the same, and the middle part lumen is thinner than upper and lower lumen, and the round bottom below the separator tube is laminated with the bottom of test tube, and the upper end opening is in the lower part of plug, with the plug laminating, the outer wall of separator tube and the inner wall laminating of test tube, compared with prior art, the utility model, its advantage lies: can go on gathering and separating in same container, the operation process is more convenient, and the while reduces the pollution chance to obtain the rich platelet of better quality, the design of density separation ball and density separation gel can guarantee when follow-up absorption rich platelet, can not absorb the red blood cell layer, guarantees the pureness of rich platelet plasma.

Description

Platelet-rich plasma collecting and separating container
Technical Field
The utility model relates to a blood collection separation technical field is a separating vessel is gathered to rich platelet plasma particularly.
Background
The platelet-rich plasma (PRP) concept was first proposed in 1993 years by Hood, and found to contain abundant platelets, the number of which is more than 3 times higher than that in whole blood.
Rich platelet separator who appears in the market at present generally will gather and separate into a plurality of devices with the separation, at first need gather blood injection separator tube with the syringe, obtain rich platelet plasma after centrifugation many times, operation process is comparatively loaded down with trivial details, and need multiple device, although used special vacuum test tube to gather rich platelet simultaneously, but the extraction of rich platelet, present open type operation, the sample of gathering is contaminated very easily, activate the platelet easily, finally lead to the curative effect not good and cause the adverse reaction because of the pollution, influence biological treatment effect.
China discloses an invention patent: collection containers for mononuclear cells and platelet rich plasma, patent No.: 201410015216.5, the patent comprises an outer tube and an inner tube, a support member is arranged in the outer tube, the inner tube is arranged in the outer tube through the support member, the inner wall of the outer tube is closely contacted with the outer wall of the inner tube, but the outer tube is not locked, and the inner tube can be easily pulled out. However, the patent is only a device for the collection step, and other devices are still selected in the separation step.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to solve the not enough of prior art, provide an album and collect and separate as an organic whole, can guarantee the collection separation vessel's of rich platelet quality technical scheme.
In order to realize above-mentioned purpose, design a separation vessel is gathered to rich platelet plasma, including test tube 2 and plug 1, its characterized in that: the inside separator tube 3 that is equipped with of test tube 2, inside separator tube 3 lined in test tube 2, be round bottom hollow container, divide upper and middle and lower three-section, the upper end opening, the semicircular seal of going to the bottom, the upper and lower lumen diameter of separator tube 3 is the same, and the middle part lumen is thinner than upper and lower lumen, and the round bottom below the separator tube 3 laminates with the bottom of test tube 2, and the upper end opening is in the lower part of plug 1, laminates with plug 1, and the outer wall of separator tube 3 laminates with the inner wall of test tube 2.
The platelet-rich plasma collecting and separating container is characterized in that: the upper portion of the rubber plug 1 is provided with a puncture hole, the lower portion of the rubber plug is connected with the opening portion of the test tube 2, and the lower portion of the rubber plug 3 is in interference fit with the opening portion of the test tube 2.
Design a separating vessel is gathered to rich platelet plasma, includes test tube 2 and plug 1, its characterized in that: the separation tube 3 is arranged in the test tube 2, the separation tube 3 is lined inside the test tube 2 and is a round bottom hollow container, the separation tube is divided into an upper section, a middle section and a lower section, the upper end is open, the lower bottom is in semicircular seal, the diameters of the upper tube cavity and the lower tube cavity of the separation tube 3 are the same, the middle tube cavity is thinner than the upper tube cavity and the lower tube cavity, the round bottom below the separation tube 3 is attached to the bottom of the test tube 2, the upper end is open at the lower part of the rubber plug 1 and is attached to the rubber plug 1, the outer wall of the separation tube 3 is attached to the inner wall of the test tube 2, the separation tube 3 is also internally provided with the density separation ball 6, the diameter of the density separation ball 6 is larger than the middle tube cavity of the separation tube 3 and.
The platelet-rich plasma collecting and separating container is characterized in that: the density separation sphere 6 is an elastic hollow sphere.
Compared with the prior art, the utility model, its advantage lies in:
1. can carry out collection and separation in same container, the operation process is more convenient, reduces the pollution chance simultaneously to obtain the better rich platelet of quality.
2. The design of the density separation ball and the density separation gel can ensure that the red blood cell layer can not be sucked when the platelet rich plasma is subsequently sucked, and ensure the purity of the platelet rich plasma.
3. The platelet rich can be concentrated in the middle thin tube only by one-time centrifugation, and the platelet rich can be conveniently collected.
Drawings
FIG. 1 is a schematic structural view of a solution of the present invention;
FIG. 2 is a diagram of a state of use of the first embodiment of the present invention;
FIG. 3 is a schematic structural view of a second embodiment of the present invention;
FIG. 4 is a diagram of a second usage state of the solution of the present invention;
in the figure: 1. the rubber plug 2, the test tube 3, the separating tube 4, the additive 5, the density separation gel 6, the density separation sphere 7, the platelet poor plasma 8, the platelet rich plasma 9 and the red blood cells.
Detailed Description
The technical scheme is further explained by combining the attached drawings.
The first embodiment is as follows: see fig. 1 and 3
Assembling a platelet rich collection separation container: firstly, the separation tube 3 is arranged in the test tube 2, the outer wall of the separation tube 3 is tightly attached to the inner wall of the test tube 2, then the density separation gel 5 is filled at the bottom of the test tube 2, the additive 4 is added according to the requirement, the rubber plug 1 is covered, the rubber plug 1 is provided with a ventilation gap, and the rubber plug 1 is compressed after the vacuum negative pressure is preset by using a vacuum negative pressure device according to the requirement.
Rubber plug 1: by the butyl rubber halide compression molding who possesses sealed function, there is the puncture hole on the upper portion of plug 1, and the lower part links to each other with test tube 2's opening, 1 lower part of plug and 2 opening interference fit of test tube to guarantee the leakproofness of whole container.
Test tube 2: the test tube is formed by injection molding of glass or high polymer transparent materials (such as polyethylene terephthalate (PET), polybutylene terephthalate (PBT) or Polycarbonate (PC) and the like) with a gas sealing function, an opening at the upper part of the test tube 2 is connected with the rubber plug 1, and the bottom of the test tube 2 is sealed to be semicircular.
Separating tube 3: the tube is arranged inside the test tube 2 and is a round bottom hollow container which is divided into an upper section, a middle section and a lower section, the upper end is opened, and the lower bottom is sealed in a semicircular shape. The upper and lower lumen diameter of separator tube 3 is the same, and the middle part lumen is than about the lumen thin, and the bottom laminating of round bottom and test tube 2 below the separator tube 3, upper end opening in the lower part of plug 1, with the laminating of plug 1, the outer wall of separator tube 3 and the inner wall laminating of test tube 2, the optional transparent macromolecular material of separator tube 3, such as polyethylene, polystyrene, polypropylene etc..
Additive 4: the anticoagulant is prepared from liquid or solid anticoagulant, such as sodium citrate, ACD anticoagulant (citric acid, sodium citrate, glucose), heparin, dipotassium ethylenediamine tetraacetate, etc., and preferably contains sodium citrate when collecting platelet rich.
Density separation gel 5: hydrophobic organogel with a certain viscosity and thixotropic activity, having a density between red blood cells and platelets, preferably between 1.050 and 1.080.
Density separation sphere 6: the hollow round ball with certain elasticity has the density between red blood cells and blood platelets, the diameter of the hollow round ball is larger than the middle lumen of the separation tube 3 and smaller than the diameter of the lower part of the separation tube 3, and the density separation round ball 6 can move freely in the lower lumen. The density separation ball 6 is made of high molecular elastic material and is in a hollow structure through injection molding, so that the density can be adjusted according to the size of the hollow structure.
Example two: see FIG. 2
The blood is fully mixed after being collected, and the plasma is separated by a horizontal centrifuge (centrifugal force 1700-1900g, 15-20 minutes), the lowest part of the separation tube 3 is erythrocyte 9 with the highest density (density about 1.093), the lower part of the separation tube is at the lower part of the separation tube, the upper part of the separation tube 3 is platelet-poor plasma 7 with the lowest density (density about 1.02), the thin tube in the middle of the separation tube 3 is platelet-rich plasma 8 with medium density (density about 1.04), and the density separation gel 5 (density about 1.05-1.08) is under the neck part at the joint of the lower part and the middle part of the separation tube 3. The platelet-rich plasma 8 is arranged above the density separation gel 5, and the red blood cells 9 are arranged below the density separation gel 5, so that the subsequent collection of the platelet-rich plasma 8 is facilitated due to the blocking effect of the density separation gel 5.
Internal volume setting of the container: the interior of the container is divided into three sections, the upper section and the lower section are thicker, the middle section is slender, the lower section is mainly a red blood cell layer after blood collection and centrifugation, and according to the specific volume of red blood cells of a human body, the normal male is 0.40-0.50, and the female is 0.35-0.45. The volume of the red blood cell layer can be set to 0.40 as the number of the intermediate portions, and the volume of each portion can be set according to the target blood collection amount when the internal volume of the separation container is set based on this number.
The proportion of the additive to the blood volume is as follows: the main component of the additive is anticoagulant, the anticoagulant is provided in a liquid form to be beneficial to uniform mixing after blood sampling, and the ratio of the additive to the blood volume is recommended to be 1: 20-1: 9, too small volume of the additive is not beneficial to the uniform mixing of blood, too much additive can cause blood dilution, and is not beneficial to the collection of platelets.
Matching the use amount of the density separation gel with the diameter of the separation tube: after centrifugation, the density separation gel should be able to completely block the cross section of the separation tube, the height of the density separation gel is preferably 5-10 mm to ensure complete separation of the red blood cell layer and the platelet rich layer, and the required weight of the density separation gel is calculated according to the diameter of the lower section of the separation tube.
Blood collection and platelet rich plasma separation: the blood sampling device is characterized in that a vein blood taking needle is used, one end of the vein blood taking needle is connected with a vein, after puncture, a tube plug puncture needle is inserted into a rubber plug puncture hole of a platelet-rich plasma collecting separator, blood can be automatically sucked into the tube under the action of negative pressure in the tube, and the blood is fully sampled according to preset vacuum negative pressure capacity and then uniformly mixed with a specimen. Centrifuge using a horizontal centrifuge, 1700g centrifugal force for 20 minutes. After centrifugation, the lower part of the separation tube 3 is packed with red blood cells 9, the density separation gel 5 is located above the red blood cells 9, the middle tubule is packed with platelet-rich plasma 8, and the upper part is packed with platelet-poor plasma 7. According to the use requirement, firstly, a syringe with an elongated injection needle tube is used for sucking the platelet-poor plasma 7 at the upper part of the separation tube 3, the platelet-rich plasma 8 in the middle thin tube is reserved, and then the syringe with the elongated injection needle tube is used for sucking the platelet-rich plasma 8 in the middle of the tube body.
Example three: see fig. 4
Fully mixing the blood after collection, separating the plasma by a horizontal centrifuge (centrifugal force of 700-1900g for 15-20 minutes), wherein the lowest part of the separation tube 3 is red blood cells 9 with the highest density (the density is about 1.093), the lower part of the separation tube 3 is arranged at the upper part of the separation tube 3 is platelet-poor plasma 7 with the lowest density (the density is about 1.02), the thin tube in the middle of the separation tube 3 is platelet-rich plasma 8 with the medium density (the density is about 1.04), the density separation round balls 6 (the density is about 1.05-1.08) are arranged at the neck part at the joint of the lower part and the middle part of the separation tube 3, due to the effect of centrifugal force, the density separation round ball 6 is clamped at the upper neck part at the lower part of the separation tube 3, platelet-rich plasma 8 is arranged above the density separation round ball 6, red blood cells 9 are arranged below the density separation round ball 6, and the subsequent platelet-rich plasma collection is facilitated due to the blocking effect of the density separation round ball 6.

Claims (4)

1. The utility model provides a separating vessel is gathered to rich platelet plasma, includes test tube (2) and plug (1), its characterized in that: be equipped with separator tube (3) in test tube (2), inside separator tube (3) line in test tube (2), be round bottom hollow container, divide upper and middle and lower three-section, the upper end opening, lower bottom semicircular seal, the upper and lower lumen diameter of separator tube (3) is the same, the middle part lumen is thinner than upper and lower lumen, round bottom below separator tube (3) is laminated with the bottom of test tube (2), the upper end opening is in the lower part of plug (1), laminate with plug (1), the outer wall of separator tube (3) and the inner wall laminating of test tube (2).
2. A platelet rich plasma collection and separation container as recited in claim 1 wherein: the upper portion of the rubber plug (1) is provided with a puncture hole, the lower portion of the rubber plug is connected with the opening portion of the test tube (2), and the lower portion of the rubber plug (1) is in interference fit with the opening portion of the test tube (2).
3. The utility model provides a separating vessel is gathered to rich platelet plasma, includes test tube (2) and plug (1), its characterized in that: be equipped with separator tube (3) in test tube (2), inside separator tube (3) line in test tube (2), be round bottom hollow container, divide into upper, middle and lower three-section, the upper end opening, lower bottom semicircular seal, the upper and lower lumen diameter of separator tube (3) is the same, and the middle part lumen is thinner than upper and lower lumen, and the round bottom below separator tube (3) is laminated with the bottom of test tube, and the upper end opening is in the lower part of plug (1), laminates with plug (1), and the outer wall of separator tube (3) and the inner wall laminating of test tube (2), still be equipped with density separation ball (6) in separator tube (3), density separation ball (6) diameter is greater than separator tube (3) middle part lumen, is less than separator tube (3) lower part lumen, can freely move in separator tube (3) lower part lumen, and the density of density separation ball (6) is between erythrocyte and platelet.
4. A platelet rich plasma collection and separation container as recited in claim 3 wherein: the density separation round ball (6) is a hollow round ball with elasticity.
CN201920810831.3U 2019-05-31 2019-05-31 Platelet-rich plasma collecting and separating container Active CN210384938U (en)

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CN201920810831.3U CN210384938U (en) 2019-05-31 2019-05-31 Platelet-rich plasma collecting and separating container

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Application Number Priority Date Filing Date Title
CN201920810831.3U CN210384938U (en) 2019-05-31 2019-05-31 Platelet-rich plasma collecting and separating container

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110064230A (en) * 2019-05-31 2019-07-30 朱德新 A kind of platelet rich plasma acquisition separation vessel

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110064230A (en) * 2019-05-31 2019-07-30 朱德新 A kind of platelet rich plasma acquisition separation vessel

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