CN215611676U

CN215611676U - Cellular-type blood cell centrifuging tube

Info

Publication number: CN215611676U
Application number: CN202121356567.4U
Authority: CN
Inventors: 李勇; 谢海涛; 薛卫巍; 苏道庆
Original assignee: Dongguan Zailijian Biotechnology Co ltd
Current assignee: Dongguan Zailijian Biotechnology Co ltd
Priority date: 2021-06-17
Filing date: 2021-06-17
Publication date: 2022-01-25
Anticipated expiration: 2031-06-17

Abstract

The utility model discloses a separated blood cell centrifuge tube, which comprises a tube body with a tube cavity and a tube cover; the tube cover is used for sealing and covering the opening end of the tube cavity; the tube cavity is also internally provided with a transverse partition plate made of elastic materials, and the transverse partition plate divides the tube cavity into an upper tube cavity and a lower tube cavity; the transverse partition plate is provided with a convex nozzle pointing to the upper pipe cavity, the convex nozzle is provided with a micropore, and the micropore enables the upper pipe cavity and the lower pipe cavity to form one-way conduction from top to bottom; the transverse partition plate made of elastic materials enables the micropores on the convex mouth to be opened under the action of centrifugal force, the red blood cell layer enters the lower tube cavity, the micropores are closed after the centrifugal force is removed, and then the plasma layer and the red blood cell layer are effectively isolated.

Description

Cellular-type blood cell centrifuging tube

Technical Field

The utility model relates to a blood cell separation preparation device, in particular to a separated blood cell centrifuge tube.

Background

Centrifugation of blood components is a commonly used procedure in medical testing and in human cell separation. The blood is centrifuged, generally by injecting the blood into a centrifuge tube, rotating the centrifuge tube at a high speed in a centrifuge to stratify the blood components, and then extracting the required stratified fluid. In the blood cell layer extraction experiment, the supernatant, i.e., the plasma layer (including cells and platelets), after the centrifugation treatment is taken, and the lower layer, i.e., the red blood cell layer, is removed.

The upper liquid is taken after the separation of the existing centrifugal tube, and the mode of extracting by an injector and obliquely pouring out by the centrifugal tube is generally adopted. The former method is complicated to operate; in the latter mode, the lower layer liquid, namely the red blood cells, is easy to pour out in the pouring process, so that red blood cell impurities are mixed in the upper layer liquid.

SUMMERY OF THE UTILITY MODEL

Based on the above problems, the present invention provides a separation type blood cell centrifuge tube, which can remove red blood cell layer and retain plasma layer by simple operation after blood is centrifuged.

The technical scheme of the utility model is as follows:

a separated blood cell centrifuge tube comprises a tube body and a tube cover, wherein the tube body is provided with an open tube cavity; a transverse partition plate made of elastic materials is arranged in the tube cavity and divides the tube cavity into an upper tube cavity and a lower tube cavity; the transverse partition plate is provided with a convex nozzle pointing to the upper tube cavity, and micropores are formed in the convex nozzle and are used for realizing one-way conduction between the upper tube cavity and the lower tube cavity from top to bottom.

In one embodiment, in the cellular blood cell centrifuge tube, a flow guide cavity is arranged on the side wall of the tube body, a flow guide through is formed between the flow guide cavity and the tube cavity of the tube body, and the depth of the flow guide cavity is the same as that of the tube cavity; the pipe cover simultaneously covers the opening end of the pipe cavity and the opening end of the flow guide cavity in a sealing manner;

in one embodiment, in the cellular-type blood cell centrifuge tube, a plurality of flow guide holes are formed in the lower side wall of the tube body, and the tube cavity is communicated with the flow guide cavity through the flow guide holes.

In one embodiment, in the cellular-type blood cell centrifuge tube, the transverse partition plate made of the elastic material is a silica gel transverse partition plate or a rubber transverse partition plate.

After the cellular blood cell centrifuge tube provided by the utility model is filled with blood for centrifugation, the convex mouth of the transverse partition plate made of elastic material in the centrifuge tube is turned over and recessed under the action of centrifugal force, and red blood cells, granulocytes and the like in the blood enter the lower tube cavity of the tube cavity in the centrifuge tube through the micropores on the recessed convex mouth; the upper tube cavity of the centrifugal tube is a plasma layer, and the lower tube cavity of the centrifugal tube is a red blood cell layer; because the transverse partition plate has certain elasticity, the convex mouth can realize that the upturned bulge points to the upper tube cavity and the sunken bulge points to the lower tube cavity under the action of certain external force, and then the plasma layer and the red blood cell layer are effectively isolated.

Drawings

FIG. 1 is a schematic view of a cellular-type blood cell centrifuge tube according to an embodiment of the present invention;

FIG. 2 is a schematic view of a cellular-type blood cell centrifuge tube according to another embodiment of the present invention;

FIG. 3 is a schematic structural diagram of a partitioned blood cell centrifuge tube before blood centrifugation;

FIG. 4 is a schematic structural diagram of a partitioned blood cell centrifuge tube after centrifugation of blood;

FIG. 5 is a schematic view of a transverse partition plate before centrifugation of the divided blood cell centrifuge tube A in FIG. 1;

FIG. 6 is a schematic diagram of the structure of the transverse partition plate after centrifugation of the divided blood cell centrifuge tube A in FIG. 1.

Detailed Description

The preferred embodiments of the present invention will be described in further detail with reference to the accompanying drawings.

As shown in fig. 1, the present invention provides a cellular centrifuge tube 100, which comprises a tube body 120 and a tube cover 110; the tube 120 is provided with an open lumen 101. The outer shape of the tube body 120 is formed by combining an upper cylinder 1202 and a lower cone 1201. The tube body 120 and the tube cover 110 are generally made of medical PVC (polyvinyl chloride), PC (polycarbonate), glass, or the like.

As shown in fig. 1, 5 and 6, in the above-mentioned cellular centrifuge tube 100, after the blood 130 is centrifuged, in order to effectively distinguish and isolate the upper plasma layer 132 (including plasma, monocyte, leukocyte, platelet and density solution, etc.) and the lower red blood cell layer 131 (including red blood cell and granulocyte density solution, etc.), it is necessary to provide a transverse partition plate 122 in the lumen 101, the transverse partition plate 122 divides the lumen 101 into an upper lumen 104 and a lower lumen 103, the transverse partition plate 122 is provided with a convex mouth 1221 pointing to the upper lumen 104, and the convex mouth 1221 is provided with micropores 1222. Since the transverse partition 122 is made of a material with a certain elastic restoring force, such as silica gel, rubber, etc. When blood 130 is filled in the upper lumen 104 of the centrifuge tube, the convex mouth 1221 of the transverse partition 122 is turned upside down and downwards and is directed towards the lower lumen 103 under the action of centrifugal force, red blood cells, granulocytes and the like in the blood 130 pass through the micropores 1222 on the concave convex mouth 1221, the diameter of the micropores 1222 is substantially similar to the particle diameter of the red blood cells in the blood, so that the red blood cells can pass through, and the micropores 1222 can enable components in the liquid red blood cell layer to overcome the surface tension formed by the liquid red blood cell layer on the micropores 1222 and enter the lower lumen 103 under the action of centrifugal force; the upper tube cavity 103 in the centrifugal tube is a plasma layer 132, and the lower tube cavity 103 in the centrifugal tube is a red blood cell layer 131; the micropores 1221 form a one-way conduction channel from top to bottom between the upper tube cavity 104 and the lower tube cavity 103, that is, after the blood 130 is centrifuged, the plasma layer 132 in the upper tube cavity 104 cannot flow to the lower tube cavity 103 through the micropores 1222 on the transverse partition 122 by its own gravity; thus, the plasma layer 132 is cleanly retained in the upper lumen 104 when the lower red blood cell layer 131 is separated and removed. Because the transverse partition 122 has certain elasticity, the convex mouth 1221 can realize that the upward-turning convex is directed to the upper tube cavity and the downward-turning convex is directed to the lower tube cavity under the action of certain external force, such as centrifugal force; after the centrifugal force is removed, the lateral barrier 122 seals the micro holes 1222 by its elastic restoring force. Therefore, when the upper tube cavity plasma layer component is poured out or taken out, the lower tube cavity red blood cell layer component can be prevented from entering the plasma layer, and the plasma layer and the red blood cell layer are effectively isolated; therefore, the separation effect of the blood cells is greatly improved, and the operation is simply realized by directly pouring out the red blood cell layer.

As shown in fig. 2 to 4, in another embodiment, in the cellular centrifuge tube 100, a diversion cavity 102 is formed in a sidewall of the tube body 121, and a diversion communication, including an air flow communication and a liquid flow communication, is formed between the diversion cavity 102 and the tube cavity 101 of the tube body 120. A plurality of flow guiding holes 1231 are formed in the lower sidewall of the tube body 120, that is, the lower cone 1021, and the flow guiding holes 1231 connect the tube cavity 101 and the flow guiding cavity 102 in a penetrating manner. The diversion cavity 102 is a cavity formed by the diversion shell 121 and the outer wall of the tube body 120, and the volume of the diversion cavity 102 is about 30% -50% of the volume of the tube cavity 101, which is beneficial to separating and removing the lower layer red blood cell layer 131 after the blood 130 in the cellular blood cell centrifuge tube 100 is centrifuged. The diversion hole 1231 is disposed in the lower cone 1201 of the tube 121 and located at the joint of the lumen 101 and the diversion cavity 102.

Preferably, the diameter of the flow guide hole 1231 is 5-10 micrometers; thus, during the blood centrifugation process, the red blood cells can pass through the blood centrifugation process.

Preferably, the depth of the diversion cavity 102 is the same as the depth of the lumen 101, that is, the open end 1021 of the diversion cavity 102 is flush with the open end 1011 of the lumen 101; thus, the cap 110 can fit over both the open end 1011 of the sealed cap lumen 101 and the open end 1021 of the diversion lumen 102.

Taking the cellular-type blood cell centrifuge tube 100 shown in fig. 2 to 4 as an example, the usage flow is as follows:

1. firstly, injecting a lymph separation liquid and/or an anticoagulant into a lower tube cavity 103 of a tube body 120 of a centrifuge tube 100 through an opening end 1011 of a tube cavity 101, and discharging air in the lower tube cavity 103 through a diversion cavity 102 and an opening end 1021 of the diversion cavity 102 in sequence;

2. then, injecting the blood 130 into the upper tube cavity 104 of the tube body 120 of the centrifuge tube 100 through the open end 1011 of the tube cavity 101; as shown in fig. 3;

3. after the tube cap 110 is sealed and covered, the centrifuge tube 100 is placed in a centrifuge for centrifugation according to the conventional operation of blood cell centrifugation; at this time, the blood 130 is centrifuged into an upper plasma layer (including monocytes, platelets, leukocytes, etc.) 132 and a lower red blood cell layer 131; as shown in fig. 4;

4. finally, the upper plasma layer (including monocytes, platelets, leukocytes, etc.) 132 is decanted or removed for use, and the lower red blood cell layer is removed.

It should be understood that the above description is illustrative of the preferred embodiment of the present invention and is not to be construed as limiting the scope of the utility model, which is defined by the appended claims.

Claims

1. A separated blood cell centrifugal tube is characterized by comprising a tube body and a tube cover, wherein the tube body is provided with an open tube cavity, and the tube cover is used for sealing and covering the open end of the tube cavity; a transverse partition plate made of elastic materials is arranged in the tube cavity and divides the tube cavity into an upper tube cavity and a lower tube cavity; the transverse partition plate is provided with a convex nozzle pointing to the upper tube cavity, and micropores are formed in the convex nozzle and are used for realizing one-way conduction between the upper tube cavity and the lower tube cavity from top to bottom.

2. The cellular-type blood cell centrifuge tube of claim 1, wherein the transverse partition made of elastic material is a silica gel transverse partition or a rubber transverse partition.