SUMMERY OF THE UTILITY MODEL
The embodiment of the application provides a blood separation utensil can make operating personnel conveniently extract rich platelet plasma, has reduced the acquisition degree of difficulty of rich platelet plasma.
The blood separation apparatus provided by the embodiment of the application comprises a centrifugal cylinder, a first cover body and a second cover body;
the first end of the centrifugal cylinder is provided with a first opening, the first cover body is in threaded connection with the first end of the centrifugal cylinder, and the first cover body closes the first opening;
the centrifugal cylinder is provided with a first blocking part, the first blocking part is close to the first opening, and a first accommodating cavity is formed among the first blocking part, the side wall of the centrifugal cylinder and the first cover body;
the second end of the centrifugal cylinder is provided with a second opening, the second cover body is in threaded connection with the second end of the centrifugal cylinder, and the second cover body closes the second opening;
the centrifugal cylinder is provided with a second blocking part, the second blocking part is close to the second opening, and a second accommodating cavity is formed among the second blocking part, the side wall of the centrifugal cylinder and the second cover body;
a centrifugal cavity is formed between the first blocking part and the second blocking part as well as between the first blocking part and the second blocking part and between the first blocking part and the centrifugal cylinder;
the first blocking part is provided with a first flow through hole which communicates the first accommodating cavity with the centrifugal cavity; a first plug for opening and closing the first flow through hole is arranged on one side, facing the first accommodating cavity, of the first cover body;
the second blocking part is provided with a second flow through hole which communicates the second accommodating cavity with the centrifugal cavity; and a second plug for opening and closing the second flow through hole is arranged on one side of the second cover body facing the second containing cavity.
The blood separation utensil that this application embodiment provided can completely cut off red blood cell, anemia platelet plasma and rich platelet plasma in the blood in the space of difference, makes operating personnel can conveniently draw rich platelet plasma, guarantees not to draw other compositions in the blood to the acquisition degree of difficulty of rich platelet plasma has been reduced.
In a possible implementation manner, the blood separation apparatus provided in this embodiment of the present application, the first blocking portion includes a partition board disposed in the centrifuge cylinder, and the first flow through hole is a through hole disposed on the partition board.
In a possible implementation manner, in the blood separation apparatus provided in this embodiment of the present application, a surface of the partition board facing the centrifugal cavity is a slope surface, a center of the slope surface is inclined toward the first accommodating cavity, and the first flow through hole is located at a center of the slope surface.
The slope helps platelet rich plasma to flow into the first flow hole and into the first containing cavity through the first flow hole.
In a possible implementation manner, in the blood separation device provided in this embodiment of the present application, a protruding portion is disposed in the centrifugal chamber, the protruding portion is disposed on an inner wall surface of the centrifugal cylinder, and the protruding portion is close to the partition plate.
The bulge can reduce the volume that the centrifugal cavity is close to first holding chamber department, makes the rich platelet plasma who remains in the centrifugal cavity still less, reduces the waste of rich platelet plasma.
In a possible implementation manner, the blood separation apparatus provided in this embodiment of the present application, the second blocking portion includes a neck portion formed by a side wall of the centrifuge bowl shrinking toward the inside of the centrifuge bowl, and the second flow through hole is defined by an inner wall surface of the neck portion.
In a possible implementation manner, the blood separation device provided in this embodiment of the present application, a sidewall of the centrifugal cylinder has an infusion hole communicating with the centrifugal cavity, and a first sealing plug is disposed in the infusion hole.
The transfusion hole is used for inputting blood to be centrifugally separated into the centrifugal cavity, and the first sealing plug can seal the transfusion hole so as to isolate the centrifugal cavity from the external environment.
And a liquid taking hole is formed in the bottom wall of the first cover body, and a second sealing plug is arranged in the liquid taking hole.
Get the liquid hole and be used for extracting the rich platelet plasma in the first chamber that holds, the liquid hole is got in the second sealing plug can seal to isolated first chamber and external environment of holding.
In a possible implementation manner, in the blood separation device provided in this embodiment of the present application, a sealing ring is disposed between the first cover and the centrifuge tube and between the second cover and the centrifuge tube.
The sealing washer can improve the gas tightness between first lid and the centrifuge bowl and between second lid and the centrifuge bowl, prevents that external impurity from entering into first chamber and the second of holding and holds the intracavity.
In a possible implementation manner, the blood separation apparatus provided by the embodiment of the present application, an end of the first stopper facing the first through-flow hole has a first conical frustum;
one end, facing the second flow through hole, of the second plug is provided with a second tapered platform.
When the first circulation hole and the second circulation hole are closed, the head parts of the first plug and the second plug can be partially inserted into the first circulation hole and the second circulation hole, so that the first circulation hole and the second circulation hole can be tightly closed.
In a possible implementation manner, the blood separation apparatus provided in the embodiments of the present application, the centrifugal cylinder includes a first cylinder and a second cylinder, and one end of the first cylinder is connected with one end of the second cylinder in a threaded manner;
the first barrel is provided with a first opening at one end far away from the second barrel, and the second barrel is provided with a second opening at one end far away from the first barrel.
The centrifugal cylinder consists of a first cylinder body and a second cylinder body, and the mold opening production of the centrifugal cylinder can be facilitated. The first barrel and the second barrel are connected through threads, and the centrifugal barrel is convenient to assemble.
In a possible implementation manner, the blood separation device provided in the embodiment of the present application has a sealing ring disposed between the first cylinder and the second cylinder.
The sealing washer can improve the gas tightness between first barrel and the second barrel, prevents that external impurity from getting into the centrifugal cavity.
Detailed Description
In the related art, blood is centrifuged and then separated into a red blood cell layer, a Platelet Rich Plasma (PRP) layer, and a Platelet Poor Plasma (PPP) layer according to specific gravities of respective components in the blood. Wherein, the specific gravity of the red blood cells is the maximum, and the red blood cells sink to the bottommost part of the test tube to form a red blood cell layer; the specific gravity of the platelet-rich plasma is the second, and the platelet-rich plasma layer is formed on the red blood cell layer; the platelet poor plasma, which has the lowest specific gravity, will lie above the platelet rich plasma layer, forming a platelet poor plasma layer. Thus, the platelet rich plasma layer is located between the red blood cell layer and the platelet poor plasma layer. When extracting platelet-rich plasma, an operator needs to make the needle of the syringe needle tube penetrate through the platelet-poor plasma layer to reach the platelet-rich plasma layer for extraction. However, since the platelet-rich plasma layer is relatively thin, it is difficult to accurately insert the needle into the platelet-rich plasma layer for extraction, so that the platelet-rich plasma is difficult to obtain.
In view of the above, an embodiment of the present disclosure provides a blood separation apparatus, which includes a centrifuge tube, a first cover and a second cover. Can isolate red blood cell, anemia platelet plasma and rich platelet plasma in the blood in the space of difference, make operating personnel can conveniently extract rich platelet plasma, guarantee can not extract other compositions in the blood to the acquisition degree of difficulty of rich platelet plasma has been reduced.
Embodiments of the present application will be described in detail below with reference to the accompanying drawings.
Example one
FIG. 1 is a schematic structural view of a blood separation device according to an embodiment of the present application; FIG. 2 is an exploded view of a blood separation device according to an embodiment of the present application; FIG. 3 is a schematic cross-sectional view of a blood separation device according to an embodiment of the present application; fig. 4a to 4g are views showing a state of use of the blood separation instrument according to the embodiment of the present application.
Referring to fig. 1-3, the present application provides a blood separation apparatus, which includes a centrifuge bowl 10, a first cover 20 and a second cover 30, wherein:
the centrifuge tube 10 is a hollow tube, and two opposite ends of the centrifuge tube 10 are respectively provided with an opening communicating with the interior of the centrifuge tube 10, namely a first opening 111 located at a first end of the centrifuge tube 10 and a second opening 121 located at a second end of the centrifuge tube 10. The outer side wall of the centrifuge tube 10 near the first end and the second end thereof is provided with an external thread for connecting with the first cover body 20 and the second cover body 30, respectively.
The first lid body 20 and the second lid body 30 are common lid bodies constituted by a bottom wall and a side wall, and both inner side wall surfaces of the first lid body 20 and the second lid body 30 have internal threads. The internal thread of the first cover 20 is connected with the external thread at the first end of the centrifuge tube 10 to close the first opening 111. Likewise, the internal threads of the second cover 30 are connected with the external threads at the second end of the centrifuge bowl 10 to close the second opening 121.
The centrifuge tube 10 has a first blocking portion 112 at a portion near the first opening 111, a first accommodating cavity 20a is formed between the first blocking portion 112 and the side wall of the centrifuge tube 10 and the first cover 20, and the first accommodating cavity 20a is used for accommodating a specific certain component in the centrifuged blood, such as platelet-rich plasma.
The centrifugal cylinder 10 has a second blocking portion 122 at a portion close to the second opening 121, a second containing cavity 30a is formed between the second blocking portion 122 and the side wall of the centrifugal cylinder 10 and the second cover 30, and the second containing cavity 30a is used for containing a specific certain component, such as red blood cells, in the blood after centrifugation.
The first blocking portion 112, the second blocking portion 122 and the sidewall of the centrifuge bowl 10 enclose the space inside the centrifuge bowl 10 to form a centrifuge chamber 10a, and the centrifuge chamber 10a is an operation space for blood centrifugal separation.
The first blocking portion 112 has a first flow hole 113 for communicating the first accommodating chamber 20a with the centrifugal chamber 10a, and the first flow hole 113 is used for introducing the component in the centrifugal chamber 10a into the first accommodating chamber 20 a. A first plug 21 is disposed on a side of the first cover 20 facing the first accommodating cavity 20a, one end of the first plug 21 is connected to the bottom wall of the first cover 20, and the other end of the first plug 21 faces the first flow hole 113. By rotating the first cover 20, the first cover 20 will rotate along the external thread of the centrifuge bowl 10, so as to adjust the axial position of the first cover on the centrifuge bowl 10 and adjust the distance between the first plug 21 and the first through-flow hole 113, so as to open and close the first through-flow hole 113.
For example, when the first plug 21 abuts against the first flow through hole 113, the first plug 21 blocks the first flow through hole 113, the first flow through hole 113 is closed, the first accommodating cavity 20a and the centrifugal cavity 10a are in a blocking state, and at this time, the component in the centrifugal cavity 10a cannot flow into the first accommodating cavity 20 a; when the first plug 21 is separated from the first through hole 113, a gap is formed between the first plug 21 and the first through hole 113, the first through hole 113 is opened, the first accommodating chamber 20a is in a state of being communicated with the centrifugal chamber 10a, and the components in the centrifugal chamber 10a can flow into the first accommodating chamber 20 a.
The second blocking portion 122 has a second flow hole 123 for communicating the second accommodating chamber 30a with the centrifugal chamber 10a, and the second flow hole 123 is used for introducing the component in the centrifugal chamber 10a into the second accommodating chamber 30 a. A second plug 31 is disposed on a side of the second cover 30 facing the second accommodating cavity 30a, one end of the second plug 31 is connected to the bottom wall of the second cover 30, and the other end of the second plug 31 faces the second through-flow hole 123. By rotating the second cover 30, the second cover 30 will rotate along the external thread of the centrifuge tube 10, so as to adjust the axial position of the second cover on the centrifuge tube 10 and adjust the distance between the second plug 31 and the second flow hole 123, so as to open and close the second flow hole 123.
For example, when the second stopper 31 abuts against the second flow hole 123, the second stopper 31 blocks the second flow hole 123, the second flow hole 123 is closed, the second accommodating chamber 30a and the centrifugal chamber 10a are in a blocking state, and at this time, the component in the centrifugal chamber 10a cannot flow into the second accommodating chamber 30 a; when the second stopper 31 is separated from the second flow through hole 123, a gap is formed between the second stopper 31 and the second flow through hole 123, the second flow through hole 123 is opened, the second accommodating chamber 30a is in a state of being communicated with the centrifugal chamber 10a, and the component in the centrifugal chamber 10a can flow into the second accommodating chamber 30 a.
In the following, the method of using the blood centrifuge apparatus according to the embodiment of the present application will be described with reference to fig. 4a to 4g, and in the specific operation, the centrifugation of blood includes two separation operations, i.e., primary centrifugation and secondary centrifugation.
When the primary centrifugal separation is performed, the first cover 20 and the second cover 30 are respectively rotated to make the first plug 21 and the second plug 31 respectively plug the first flow hole 113 and the second flow hole 123, so as to ensure that the first accommodating cavity 20a and the second accommodating cavity 30a are both in a blocking state with the centrifugal cavity 10a (see fig. 4 a). Then injecting blood to be centrifugally separated into the centrifugal cavity 10a, and placing the first end of the blood separation device into a centrifugal separator to carry out centrifugal separation with the first end facing upwards, so that red blood cells with the maximum specific gravity in the blood are settled at the bottom of the centrifugal cavity 10a to form a red blood cell layer A; the plasma containing platelets in the blood is allowed to sit on the red blood cell layer a, forming a plasma layer B (see fig. 4B). Then, the second cover 30 is rotated until a gap is formed between the second plug 31 and the second through hole 123, so that the second through hole 123 is opened, and the erythrocyte layer a deposited on the bottom of the centrifugal cavity 10a flows into the second accommodating cavity 30a through the second through hole 123 (see fig. 4 c). After the erythrocyte layer a is completely discharged from the centrifugal cavity 10a to the second accommodating cavity 30a, the second cover 30 is rotated until the second plug 31 blocks the second flow through hole 123, so that the second flow through hole 123 is closed to prevent the plasma layer B from flowing into the second accommodating cavity 30 a. After the primary centrifugation, only the platelet-containing plasma layer B remains in the chamber 10a (see FIG. 4 d).
In order to obtain platelet-rich plasma having a high platelet concentration, after the primary centrifugation is completed, the blood separation instrument is turned over with its second end facing upward and placed in a centrifuge for centrifugation, and the plasma layer B is centrifuged into a platelet-rich plasma layer B1 located at the bottom of the centrifugal chamber 10a and a platelet-poor plasma layer B2 located above the platelet-rich plasma layer B1 (see fig. 4 e). Then, the first cover 20 is rotated until there is a gap between the first stopper 21 and the first flow hole 113, so that the first flow hole 113 is opened, and the platelet-rich plasma layer B1 deposited on the bottom of the centrifugal chamber 10a flows into the first containing chamber 20a through the first flow hole 113 (see fig. 4 f). After the platelet-rich plasma layer B1 is completely discharged from the centrifugal chamber 10a to the first accommodating chamber 20a, the first cover 20 is rotated until the second plug 31 blocks the first flow hole 113, so that the first flow hole is closed to prevent the platelet-rich plasma layer B2 from flowing into the first accommodating chamber 20 a. After the second centrifugation, the platelet-poor plasma layer B2 remains in the centrifugal chamber 10a, and the platelet-rich plasma layer B1 remains in the first holding chamber 20a (see fig. 4 g). At this time, the platelet rich plasma in the first containing cavity 20a can be conveniently extracted, and other components in the blood are not extracted, so that the difficulty in obtaining the platelet rich plasma is reduced.
The blood separation utensil of this application embodiment can isolate red blood cell, anemia platelet plasma and rich platelet plasma in the blood in the space of difference, makes operating personnel can conveniently extract rich platelet plasma, guarantees not to extract other compositions in the blood to the acquisition degree of difficulty of rich platelet plasma has been reduced.
In addition, the first cover body 20 and the second cover body 30 can be rotated to isolate red blood cells, platelet poor plasma and platelet rich plasma in different spaces, and the operation mode is simple and easy to learn. Meanwhile, only one injection process and one extraction process are carried out in the operation process, so that the isolation between the blood and the external environment is ensured to a great extent, and the blood can be prevented from being polluted by external components.
In order to facilitate the operation, the centrifuge bowl 10, the first cover 20 and the second cover 30 of the blood separation device are made of transparent materials, so that the operator can observe the distribution and flow of blood components in each layer of the blood separation device conveniently. On the basis, corresponding scale marks can be arranged on the centrifugal cylinder 10, the first cover body 20 and the second cover body 30 to further facilitate the operation.
Referring to fig. 1-3, in the embodiment of the present application, a centrifugal cylinder 10 includes a first cylinder 11 and a second cylinder 12, and one end of the first cylinder 11 is screwed with one end of the second cylinder 12. The first opening 111 is arranged at one end of the first cylinder 11 far away from the second cylinder 12, and the second opening 121 is arranged at one end of the second cylinder 12 far away from the first cylinder 11. The centrifugal cylinder 10 is composed of a first cylinder body 11 and a second cylinder body 12, and can facilitate the mold opening production of the centrifugal cylinder 10. The first cylinder 11 and the second cylinder 12 are connected by screw threads, which also facilitates the assembly of the centrifuge cylinder 10.
In addition, a sealing ring (not shown) is arranged between the first cylinder 11 and the second cylinder 12, and the sealing ring can improve the air tightness between the first cylinder 11 and the second cylinder 12 and prevent external impurities from entering the centrifugal cavity 10 a.
FIG. 5 is a schematic cross-sectional view of a first cartridge of the blood separation device of an embodiment of the present application; FIG. 6 is a schematic structural view of a first sealing plug in the blood separation device according to the embodiment of the present application; fig. 7 is a schematic structural view of a second sealing plug in the blood separation device according to the embodiment of the present application.
Referring to fig. 2, 3 and 5, in the embodiment of the present application, the first blocking portion 112 includes a partition plate disposed in the centrifuge bowl 10, the partition plate is disposed on an inner side wall surface of the centrifuge bowl 10 to separate the centrifuge chamber 10a from the first accommodating chamber 20a, and the first flow through hole 113 is a through hole disposed on the partition plate.
In order to make the platelet-rich plasma flow into the first containing cavity 20a more easily, the side of the partition plate facing the centrifugal cavity 10a is a slope, the center of the slope is inclined toward the first containing cavity 20a, and the first flow through hole 113 is located at the center of the slope. This facilitates the platelet-rich plasma to flow into the first flow-through hole 113 and flow into the first containing chamber 20a through the first flow-through hole 113.
When the blood separation apparatus according to the embodiment of the present application performs the second centrifugal separation, in order to avoid the platelet-poor plasma from entering the first accommodating cavity 20a and causing the decrease of the platelet concentration in the first accommodating cavity 20a, a part of the platelet-rich plasma is usually retained in the centrifugal cavity 10a, which inevitably causes the waste of the platelet-rich plasma. Therefore, in order to reduce the waste of platelet-rich plasma, a protrusion 114 is further disposed in the centrifugal chamber 10a, the protrusion 114 is disposed on the inner wall surface of the centrifugal cylinder 10, and the protrusion 114 is close to the partition plate. In this way, the protrusion 114 can reduce the volume of the centrifugal chamber 10a near the first accommodating chamber 20a, so that the platelet-rich plasma remaining in the centrifugal chamber 10a is less, and the waste of the platelet-rich plasma is reduced.
Referring to fig. 2 and 3, in the embodiment of the present application, the second blocking portion 122 includes a neck portion formed by the side wall of the centrifuge bowl 10 shrinking toward the inside of the centrifuge bowl 10, and the second flow through hole 123 is defined by the inner wall surface of the neck portion. The side wall of the centrifuge tube 10 near the neck is contracted towards the neck, and forms a funnel shape at two ends near the neck, which can facilitate the collection of the red blood cells towards the second flow through hole 123 and the flow into the second containing cavity 30 a.
Referring to fig. 2 and 3, in the embodiment of the present application, the sidewall of the centrifuge bowl 10 has an infusion hole 115 communicating with the centrifuge chamber 10a, and the infusion hole 115 is used for infusing blood to be centrifuged into the centrifuge chamber 10 a. A first sealing plug 116 is arranged in the infusion hole 115, and the first sealing plug 116 can seal the infusion hole 115 to isolate the centrifugal cavity 10a from the external environment. The first sealing plug 116 is a soft plug made of elastic material, which facilitates the syringe needle to penetrate the first sealing plug 116 deep into the sealing cavity and input the blood to be centrifuged into the centrifugal cavity 10 a.
The bottom wall of the first cover 20 is provided with a liquid extraction hole 22, and the liquid extraction hole 22 is used for extracting platelet-rich plasma in the first accommodation cavity 20 a. The second sealing plug 23 is disposed in the liquid taking hole 22, and the second sealing plug 23 can seal the liquid taking hole 22 to isolate the first accommodating cavity 20a from the external environment. Wherein the second sealing plug 23 is a soft plug made of an elastic material, which facilitates the penetration of the syringe needle through the second sealing plug 23 into the first receiving chamber 20a for the extraction of platelet-rich plasma.
In the embodiment of the present application, sealing rings (not shown) are disposed between the first cover 20 and the centrifuge tube 10 and between the second cover 30 and the centrifuge tube 10. The sealing rings can improve the air tightness between the first cover body 20 and the centrifuge tube 10 and between the second cover body 30 and the centrifuge tube 10, and prevent external impurities from entering the first accommodating cavity 20a and the second accommodating cavity 30 a.
Referring to fig. 6 and 7, in the embodiment of the present invention, an end of the first plug 21 facing the first flow through hole 113 has a first tapered platform 211, and an end of the second plug 31 facing the second flow through hole 123 has a second tapered platform 311. When the first flow through hole 113 and the second flow through hole 123 are closed, the heads of the first plug 21 and the second plug 31 can be partially inserted into the first flow through hole 113 and the second flow through hole 123, so that the first flow through hole 113 and the second flow through hole 123 can be tightly closed.
In addition, the first plug 21 and the second plug 31 may be made of an elastic material, so that the heads of the first plug 21 and the second plug 31 can be elastically deformed when entering the first flow hole 113 and the second flow hole 123, and an outward elastic restoring force is formed, thereby further ensuring that the first flow hole 113 and the second flow hole 123 can be tightly closed.
In the description of the embodiments of the application, it is to be understood that the terms "mounted," "connected," and "connected" are to be construed broadly and may include, for example, a fixed connection, an indirect connection via intermediate media, a connection between two elements, or an interaction between two elements, unless expressly stated or limited otherwise. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations. The terms "upper", "lower", "front", "rear", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. In the description of the present invention, "a plurality" means two or more unless specifically stated otherwise.
The terms "first," "second," "third," "fourth," and the like in the description and in the claims of the present application and in the above-described drawings are used for distinguishing between similar elements and not necessarily for describing a particular sequential or chronological order. It is to be understood that the data so used is interchangeable under appropriate circumstances such that the embodiments of the application described herein are, for example, capable of operation in sequences other than those illustrated or otherwise described herein. Furthermore, the terms "comprises," "comprising," and "having," and any variations thereof, are intended to cover a non-exclusive inclusion, such that a process, method, system, article, or apparatus that comprises a list of steps or elements is not necessarily limited to those steps or elements expressly listed, but may include other steps or elements not expressly listed or inherent to such process, method, article, or apparatus.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the utility model has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may be modified, or some or all of the technical features may be equivalently replaced; and the modifications or the substitutions do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present invention.