Platelet-rich plasma centrifuge tube and extraction method thereof
Technical Field
The invention relates to the field of medical instruments, in particular to a platelet-rich plasma centrifuge tube and an extraction method thereof.
Background
Platelet Rich Plasma (PRP) can be prepared in an operating room under ordinary conditions, but the PRP can be prepared in an operating room under the condition that the sterility is maintained in the centrifugation process, the PRP needs to be separated under high concentration without platelet dissolution or damage, different PRP extraction devices have different prepared PRP results, a whole set of a plurality of devices, test tubes, syringes and the like are used in the separation and extraction process of the platelet rich plasma in medical treatment at present, the operation is quite complicated, and the platelet rich plasma is inconvenient and easy to cause pollution after being injected, centrifuged and extracted for a plurality of times, so that the method and the tool are improved, and the whole operation step is simplified and pollution-free.
Disclosure of Invention
Aiming at the defects of the prior art, the method aims to solve the problem that the existing platelet-rich plasma separation and extraction is too complicated.
In order to achieve the purpose, the invention adopts the following technical scheme:
a platelet-rich plasma centrifuge tube comprises a serum area, a platelet-rich plasma area, a white blood cell area and a red blood cell area from top to bottom in sequence;
the platelet-rich plasma area is connected with the leukocyte area through an upper through hole;
the white blood cell area is connected with the red blood cell area through a lower through hole;
the side wall of the leucocyte area is provided with an extraction hole;
the white blood cell area is provided with a three-way T-shaped switch corresponding to the upper through hole, the lower through hole and the extraction hole.
As preferred scheme, platelet rich plasma centrifuging tube formula structure as an organic whole, the bottom in serum district includes inverted circular truncated cone shape structure, platelet rich plasma district is the cylinder structure, the leucocyte district is the cylinder structure, the top in erythrocyte district includes circular truncated cone shape structure.
Preferably, the top of the serum area is provided with a tube cover.
Preferably, the tube cover is provided with an injection hole and a vent hole.
Preferably, an injection hole cover is arranged on the injection hole, and a vent hole cover is arranged on the vent hole.
Preferably, the injection hole is arranged in the central area of the tube cover, and a breathable antibacterial film is arranged in the ventilation hole.
Preferably, the bottom of the red blood cell area is provided with a rotary piston.
Preferably, a three-way T-shaped switch valve is arranged on the side wall corresponding to the three-way T-shaped switch and the leukocyte area.
In order to achieve the purpose, the invention also adopts the following technical scheme:
a method for extracting platelet rich plasma comprises the following steps:
rotating the three-way T-shaped switch valve to conduct the upper through hole and the lower through hole and seal the extraction hole;
opening the injection hole cover and the vent hole cover;
injecting the plasma into a centrifuge tube from an injection hole, and then closing the injection hole cover and the vent hole cover;
placing the centrifuge tube filled with the blood plasma into a centrifuge for centrifugation;
after the plasma is layered, opening a vent cover;
connecting the injector with the upper extraction hole, rotating the three-way T-shaped switch valve to enable the upper through hole and the extraction hole to be communicated, sealing the lower through hole, opening the vent cover, and extracting platelet-rich plasma by using the injector.
Preferably, after the plasma is layered and the vent cover is opened, the method comprises the following steps:
rotating the rotary piston to adjust the upper liquid level of the white blood cells to a white blood cell area;
if the white blood cells need to be extracted, the upper liquid level of the white blood cells is pushed to be higher than the extraction holes;
if the extraction of the white blood cells is not needed, the upper liquid level of the white blood cells is pushed to be lower than the extraction hole.
The platelet-rich plasma centrifuge tube has the beneficial effects that:
1) compared with the prior art, the platelet-rich plasma centrifuge tube provided by the invention sequentially comprises a serum area, a platelet-rich plasma area, a white blood cell area and a red blood cell area from top to bottom; the platelet-rich plasma area is connected with the leukocyte area through an upper through hole; the white blood cell area is connected with the red blood cell area through a lower through hole; the side wall of the leucocyte area is provided with an extraction hole; the leucocyte area is provided with a three-way T-shaped switch corresponding to the upper through hole, the lower through hole and the extraction hole, and the three-way T-shaped switch can enable the upper through hole, the lower through hole and the extraction hole to be in the following conduction or closed states: 1. the upper through hole and the lower through hole are communicated, and the extraction hole is closed; 2. the upper through hole is communicated with the extraction hole, and the lower through hole is sealed; 3. the upper through hole, the lower through hole and the extraction hole are all communicated; 4. go up through-hole, lower through-hole, draw the hole totally closed, make like this as required go up through-hole, lower through-hole, draw the hole and realize above switch on or the closed condition, make the extraction of rich platelet plasma centrifuging tube simpler, also difficult messenger's plasma receives the pollution.
2) Compared with the prior art, the platelet-rich plasma centrifuge tube of the platelet-rich plasma centrifuge tube has an integrated structure, the bottom of the serum area comprises an inverted circular truncated cone-shaped structure, the platelet-rich plasma area is a cylindrical structure, the white blood cell area is a cylindrical structure, the top of the red blood cell area comprises a circular truncated cone-shaped structure, the platelet-rich plasma centrifuge tube sequentially comprises the serum area, the platelet-rich plasma area, the white blood cell area and the red blood cell area from top to bottom according to the sequence that the densities of serum, platelet-rich plasma, white blood cells and red blood cells after the whole plasma is separated by a centrifuge are from small to large, and the serum area, the platelet-rich plasma area, the white blood cell area and the red blood cells are divided into different areas according to the volume ratio of the serum, the platelet-rich plasma, the white blood cells and the red blood cells after the whole plasma is separated by the centrifuge, especially, the volume ratio of white blood cells to the whole plasma is relatively small, and the white blood cell area is designed into a long and thin cylindrical structure.
3) Compared with the prior art, the tube cover is arranged at the top of the serum area of the platelet-rich plasma centrifuge tube, the tube cover is provided with the injection hole and the vent hole, the injection hole is provided with the injection hole cover, and the vent hole is provided with the vent hole cover, so that the platelet-rich plasma centrifuge tube is in a closed state, and plasma is not easily polluted.
4) Compared with the prior art, the vent hole of the platelet-rich plasma centrifuge tube is internally provided with the breathable antibacterial film, so that plasma is not easily polluted.
5) Compared with the prior art, the rotating piston is arranged at the bottom of the red blood cell area of the platelet-rich plasma centrifuge tube, and the liquid level of separated and layered plasma can uniformly rise or fall through the rotating piston.
6) Compared with the prior art, the side wall of the leucocyte area of the platelet-rich plasma centrifuge tube is provided with the three-way T-shaped switch valve, so that the three-way T-shaped switch is convenient to use.
7) Compared with the prior art, the injection hole and the extraction hole of the platelet-rich plasma centrifuge tube are respectively arranged at two different positions, so that plasma is not easily polluted.
8) Compared with the prior art, the platelet rich plasma centrifuge tube has the advantages that the three-way T-shaped switch, the injection hole cover and the vent hole cover are used, so that the platelet rich plasma centrifuge tube is more sealed, sample pollution caused in the operation process is avoided, and the stability and the safety of platelet rich plasma manufacturing are further improved.
9) Compared with the prior art, the platelet rich plasma centrifuge tube has the advantages that the bottom of the serum area comprises the inverted frustum-shaped structure, the top of the red blood cell area comprises the truncated cone-shaped structure, the two layers of gradient tube diameters are designed, the platelet rich plasma centrifuge tube is of the integrated structure, and the stability and the safety of platelet rich plasma production are further improved.
10) Compared with the prior art, the extraction method of the platelet rich plasma can rotate the rotary piston to enable the upper liquid level of the white blood cells to descend or ascend when the upper liquid level of the white blood cells is higher or lower.
Drawings
Fig. 1 is a schematic diagram of an overall structure of a platelet-rich plasma centrifuge tube according to an embodiment of the present invention.
Fig. 2 is a schematic structural view of a three-way T-switch, an upper through hole, a lower through hole and an extraction hole of a platelet-rich plasma centrifuge tube according to an embodiment of the present invention.
Fig. 3 is a flowchart of a method for extracting platelet rich plasma according to an embodiment of the present invention.
Fig. 4 is another flow chart of a method for extracting platelet rich plasma according to an embodiment of the present invention.
Description of reference numerals:
1. serum area, 2, platelet rich plasma area, 3, leukocyte area, 4, erythrocyte area, 5, upper through hole, 6, lower through hole, 7, extraction hole, 8, three-way T-shaped switch, 9, tube cover, 10, injection hole, 11, vent hole, 12, injection hole cover, 13, vent hole cover, 14, rotary piston, 15 and three-way T-shaped switch valve.
Detailed Description
The invention is further described with reference to the following figures and specific embodiments.
Referring to fig. 1 and 2, an embodiment of the present invention provides a platelet-rich plasma centrifuge tube, which sequentially includes, from top to bottom, a serum area 1, a platelet-rich plasma area 2, a white blood cell area 3, and a red blood cell area 4;
the platelet-rich plasma area 2 is connected with the leukocyte area 3 through an upper through hole 5;
the white blood cell area 3 is connected with the red blood cell area 4 through a lower through hole 6;
the side wall of the leucocyte area 3 is provided with an extraction hole 7;
the white blood cell area 3 is provided with a three-way T-shaped switch 8 corresponding to the upper through hole 5, the lower through hole 6 and the extraction hole 7.
Wherein, platelet rich plasma centrifuging tube formula structure as an organic whole, the bottom in serum district 1 includes inverted circular truncated cone shape structure, platelet rich plasma district 2 is the cylinder structure, leucocyte district 3 is the cylinder structure, the top in erythrocyte district 4 includes circular truncated cone shape structure.
Wherein, the top of the serum area 1 is provided with a tube cover 9.
Wherein, the tube cover 9 is provided with an injection hole 10 and a vent hole 11.
Wherein, an injection hole cover 12 is arranged on the injection hole 10, and a vent hole cover 13 is arranged on the vent hole 11.
Wherein, the injection hole 10 is arranged in the central area of the tube cover 9, and a breathable antibacterial film (not shown in the figure) is arranged in the vent hole 11.
Wherein, the bottom of the red blood cell zone 4 is provided with a rotary piston 14.
Wherein, a three-way T-shaped switch valve 15 is arranged on the side wall corresponding to the three-way T-shaped switch 8 and the leucocyte area 3.
Referring to fig. 3, an embodiment of the present invention provides a flow chart of a method for extracting platelet rich plasma, including the following steps:
s101, rotating a three-way T-shaped switch valve to enable an upper through hole and a lower through hole to be communicated, and sealing an extraction hole;
step S102, opening a injection hole cover and a vent hole cover;
step S103, injecting the plasma into a centrifuge tube from the injection hole, and then closing the injection hole cover and the vent hole cover;
step S104, putting the centrifugal tube filled with the plasma into a centrifugal machine for centrifugation, wherein the centrifugal machine works as a preferred scheme
The working time is 15 minutes, and the working speed of the centrifugal machine is 400 g;
step S105, opening a vent cover after the plasma is layered;
if the white blood cells need to be extracted, the height of the upper liquid level of the white blood cells is found to be higher than the extraction holes;
or if the white blood cells are not required to be extracted, finding that the height of the upper liquid level of the white blood cells is lower than the extraction holes;
the adjustment of the height of the upper liquid level of the white blood cells is not needed, and the next step is directly carried out.
And S106, connecting the injector with the upper extraction hole, then rotating the three-way T-shaped switch valve to enable the upper through hole and the extraction hole to be communicated, sealing the lower through hole, then opening the vent cover, and extracting the platelet-rich plasma by using the injector.
Referring to fig. 4, another flow chart of the method for extracting platelet-rich plasma according to the embodiment of the present invention includes the following steps:
step S201, rotating a three-way T-shaped switch valve to enable an upper through hole and a lower through hole to be communicated, and sealing an extraction hole;
step S202, opening a syringe cap and a vent cap;
step S203, injecting the plasma into a centrifuge tube from the injection hole, and then closing the injection hole cover and the vent hole cover;
step S204, putting the centrifuge tube filled with the plasma into a centrifuge for centrifugation, wherein the centrifuge works as a preferred scheme
The working time is 15 minutes, and the working speed of the centrifugal machine is 400 g;
step S205, after the plasma is layered, opening a vent cover;
if the white blood cells need to be extracted, the height of the upper liquid level of the white blood cells is found to be lower than the extraction holes;
or if the white blood cells do not need to be extracted, the height of the upper liquid level of the white blood cells is higher than the extraction holes;
the next step is to adjust the height of the upper liquid level of the white blood cells.
Step S206, rotating the rotary piston to adjust the upper liquid level of the white blood cells to a white blood cell area;
if the white blood cells need to be extracted, the upper liquid level of the white blood cells is pushed to be higher than the extraction holes;
if the white blood cells do not need to be extracted, the upper liquid level of the white blood cells is pushed to be lower than the extraction holes;
and step S207, connecting the injector with the upper extraction hole, then rotating the three-way T-shaped switch valve to enable the upper through hole to be communicated with the extraction hole, sealing the lower through hole, then opening the vent cover, and extracting the platelet-rich plasma by using the injector.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the technical scope of the present invention, so that any minor modifications, equivalent changes and modifications made to the above embodiment according to the technical spirit of the present invention are within the technical scope of the present invention.