CN103505910A - Method for preparing platelet rich plasma through one-step centrifugation method - Google Patents
Method for preparing platelet rich plasma through one-step centrifugation method Download PDFInfo
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Abstract
The invention belongs to the technical field of preparation of platelet rich plasma and in particular discloses a method for preparing PRP (Platelet Rich Plasma) through a one-step centrifugation method. According to the method, only a high-speed automatic centrifuge is utilized, the one-step centrifugation method is adopted, only one-step liquor relief (which is used for absorbing plasma on a boundary layer) is required in the whole process, and the purposes of convenience, rapidness and low pollution risk are achieved. Moreover, according to the platelet rich plasma prepared by the method, a mean value of platelet cell concentrations reaches 1029.875*10<9>/L, the mean corpuscular hemoglobin in the PRP is 20.26 g/L, and the clinical requirements can be well met.
Description
Technical field
The present invention relates to the preparation method of platelet rich plasma, particularly, relate to a kind of method that a centrifugal process is prepared platelet rich plasma.
Background technology
Platelet rich plasma (platelet-rich plasma, PRP) be the whole blood by centrifugal human or animal self obtain containing the hematoblastic blood plasma of high-volume fractional.After the eighties in last century, the people such as DAVID R. KNIGHTON found that platelet cell can promote that blood vessel hyperplasia, collagen are synthetic, people are just devoted to platelet rich plasma (PRP) to be applied to clinical, thirst for solving the wound repair problem of the low organ-tissue of repair ability.But due to the difficulty of PRP preparation at that time, limited its popularization clinically.
Through the research of decades, several comparatively suitable platelet rich plasma preparation methods have been explored: manual method (centrifugal process, secondary centrifuging method and three centrifugal process) and equipment preparation method.Wherein, the recovery rate of the PRP of secondary centrifuging method is the highest, also the widest in clinical application.Utilize secondary centrifuging legal system as follows for the principle of PRP: (1) venous blood samples liquid is also injected into containing anti-coagulants in vitro; (2) the 1st times centrifugal can be divided into blood 3 layers, and the part of lowermost end is the red blood cell that accounts for blood total volume fraction 55%; Head portion is the platelet poor plasma (platelet-poor plasma, PPP) that accounts for total volume fraction 40%, is mainly the plasma fractions such as fibrinogen; Intermediate layer is for only accounting for the platelet concentrate (platelet concentrate, PC) of total volume fraction 5%, the yellow clothes layer being commonly called as; (3) with pipettor, draw the part red blood cell of PPP and PC and next-door neighbour PC, and be injected into another not containing in the test tube of anti-coagulants; (2) again it is centrifugal and blood plasma is divided into 3 layers with certain speed and time, lowermost end is a small amount of remaining red blood cell, and top is the PPP that accounts for total volume fraction 80%, is the blood platelet of enrichment between two-layer; (5) with pipettor, extract most PPP, and leave and take enough serum and hold the blood platelet that is suspended in enrichment wherein, obtain PRP.In addition, blood platelet in PRP is more fragile and easily activation in vitro, too high centrifugal speed can make platelet membrane break to reduce its biologically active, and lower centrifugal speed can make biologically active pdgf in preparation process, remain on floor level, and when therefore centrifugal, speed is unsuitable too fast.And, in the prepared PRP of different centrifugal number of times, centrifugal force and centrifugation time, the amount of hematoblastic volume fraction and various growth factors and active different.
Though can prepare the PRP that composition is comparatively single, concentration is up to standard at present, its technology of preparing is full maturity not yet, the secondary centrifuging method that most people is praised highly exists that complicated operation, process are very long, the risk of contamination of products.The PRP equipment that China Wei Gao company is about to listing exists the not high defect of PC in PRP, the PRP equipment that the Er U.S. has gone on the market, in its PRP, HC is unexposed, therefore may exist the high but defect that purity is not high of platelet cell concentration, and the PRP equipment of each listing, prices are rather stiff for it, is being subject to larger restriction aspect clinical practice popularization, especially in the application of developing country.At present, solving the method that PRP prepares problem is still worth further probing into, to obtain a kind of convenient and swift, purity and high, the cheap preparation method of concentration.
Summary of the invention
The technical problem to be solved in the present invention overcomes current traditional-handwork legal system for platelet rich plasma concentration or purity is not high and manual secondary centrifuging method preparation process is complicated, adopt the high defect of listing device suite preparation cost, for providing a kind of, clinical research can prepare all technology of high platelet rich plasma of concentration and purity, its preparation process is easy, and cost is low.
Object of the present invention is achieved by the following technical programs:
An a kind of centrifugal process is prepared the method for platelet rich plasma, comprise the steps: the whole blood that is mixed with anti-coagulants with the centrifugal force of 1400g centrifugal 15 minutes, after centrifugal end, whole blood will be divided into 3 layers, upper strata is platelet poor plasma layer, middle level is that platelet rich plasma Ceng， lower floor is hemoglobin layer, then evenly draws middle level blood plasma and obtains platelet rich plasma.
Preferably, described anti-coagulants is heparin or hirudin.More preferably, described anti-coagulants is heparin.
Preferably, the addition of described heparin in whole blood is that 0.2:10 determines according to the volume ratio of heparin and whole blood, the heparin that described heparin is 12500 units/2mL.
Preferably, the concrete preparation method of described platelet rich plasma is: by being mixed with 1.0mL concentration, be that the 50mL whole blood of 12500 units/2mL heparin carries out centrifugal 15min minute with 1400g centrifugal force; After centrifugal end, it is that platelet poor plasma Ceng， middle level is that platelet rich plasma Ceng， lower floor is that hemoglobin Ceng，Yu middle level is evenly drawn 4mL blood plasma and obtained platelet rich plasma that whole blood will be divided into 3 Ceng， upper stratas.
The present invention only utilizes a High-Speed Automatic centrifuge, adopts centrifugal process one time, and whole process only needs once to move liquid (drawing boundary layer blood plasma), to reach the object that cost is low, easy to prepare fast, pollution risk is low.Through the research (the results are shown in subordinate list and figure) of Guangdong People's Hospital's orthopaedics, in the PRP that patent of the present invention prepares, its average platelet concentration reaches 1029.875 * 10
9/ L(is 3.8 times of whole blood PC, standard deviation is 259.92, standard is mistaken for 91.89,95% credibility interval is (812.58,1247.17)), NCHC is only for 20.26g/L(standard deviation is 15.82, standard is mistaken for 5.59,95% credibility interval is (7.03,33.49)), this just reaches purity and the high object of concentration.The PRP that patent system of the present invention is standby, only utilizes a supercentrifuge, and preparation consumes naturally few compared with adopting the device suite preparation cost of listing, therefore has advantages of cheap.
The method of the invention is better than a traditional centrifugal process (Anitua method): 1999 Nian， Spain Eduardo doctors Anitua, invented Anitua method.He gathered patient's 10 to 20mL venous blood at that time, add in a plurality of centrifuge tubes of 5mL, with 160g centrifugal force centrifugal 6 minutes, then abandon or adopt upper strata (platelet poor plasma layer) 1ml, draw residue upper plasma to the 1～2mm of lower floor's (red blood cell layer), the venous blood of 5mL approximately can obtain the PRP of 1.2mL so again.He analyzed (n=10) at that time, in the upper plasma discarding, platelet cell quantity is only less than whole hematoblastic 15%, still have more blood platelet remain in upper strata and discarded as seen, show that Anitua method takes centrifugation time and centrifugal force not enough so that most of blood platelet falls to middle level.The centrifugation time of the technology of the present invention and centrifugal force, all compared with Anitua fado, can make the blood platelet that is stranded in upper strata be settled down to middle level.
Compare with secondary centrifuging method (Landesberg method): during the standby PRP of secondary centrifuging legal system, first centrifugal employing low-speed centrifugal, then gets blood plasma more than 3mm under interface, this is inevitable remaining a large amount of hemoglobin that have wherein.And after high speed centrifugation for the second time, draw whole lower floors blood plasma, and being PRP, this has wherein comprised first centrifugal rear residual hemoglobin.In the PRP preparing with Landesberg method, hemoglobin content is high.In addition, secondary centrifuging method needs many through once moving liquid, and preparation process is complicated, and pollution risk is high.
Compare with existing platelet rich plasma preparation method, Preparation equipment, the present invention has following beneficial effect:
(1) centrifugal process of the preparation method of platelet rich plasma of the present invention and tradition is compared, concentration and the purity of the PRP preparing are higher, compare convenient, fast with secondary centrifuging method, greatly reduce operating time and pollution risk that manual method is prepared platelet rich plasma.15 minutes consuming time of the technical method of patent of the present invention, and the Landesberg method of praising highly the most in secondary centrifuging method is consuming time more, need to be for twice 10 minutes centrifugation times, add the operating time that the absorption of extra 1 time moves liquid, and, secondary is drawn and is moved the complexity that liquid process has not only increased operation, has more seriously increased opportunities for contamination.The technical method of patent of the present invention only need 1 time centrifugal, avoided drawing for the second time the process of moving liquid, operate more easyly, the more important thing is and greatly reduce the directly duration of exposure of blood plasma.
(2) in the platelet rich plasma that prepared by the technical method of patent of the present invention, platelet cell concentration average reaches 1029.875 * 10
9/ L.It is 1204.8333 * 10 that Er Ben research unit utilizes platelet cell concentration in the PRP that Landesberg method prepares
9, both relatively do not have notable difference; But on the other hand, utilizing hemoglobin content in the PRP that the technical method of patent of the present invention prepares is 20.26g/L, and in the PRP that Landesberg method prepares, hemoglobin content is 59.533g/L, and both have compared notable difference (P=0.023).And platelet cell concentration average is 819.47 * 10 in PRP prepared by the preparation system of China Wei Gao company
9(Li Ming, Zhang Changqing, Yuan Ting etc.: the evaluation studies [J] of platelet rich plasma preparation suit. Chinese Reconstructive surgery magazine .2011,25 (1) 112-116), the PC that therefore preparation is originally issued in utilization is obviously compared with the height of China's listing device suite.
(3) patented technology method of the present invention will be carried out by Yu Wo institute orthopaedics, and the whole therapeutic process of patient of tentatively drafting approximately spends 3500 yuan.And the PRP equipment of listing, only be to prepare PRP just to need 300～400 dollars, the suit price of GenesisCS device systems of also not calculating the other fees ，Ru U.S. of patient treatment process is 1550 dollars, and the suit price that China Wei Gao company is about to the PRP device systems of listing is 8000 yuan.Therefore, with the technical method of patent of the present invention, prepare PRP, with respect to other device systems, more economical.
figure of description
Fig. 1. the average of platelet count in the PRP that 17 kinds of centrifugal treating methods prepare.
Fig. 2. platelet count result in the PRP of 17 groups and whole blood.
Fig. 3. PLT and HGB average in the PRP that 15-1400 method of the present invention and Landesberg method obtain.
The specific embodiment
Below in conjunction with the drawings and specific embodiments, further illustrate the present invention.The test method of using in embodiment if no special instructions, is conventional method; The material using, reagent etc. if no special instructions, are reagent and the material that can obtain from commercial channels.
Because the platelet rich plasma preparing (PRP) finally will be with being back to human body, so the overall process of preparation PRP needs strictly aseptic.
Embodiment 1
S1. materials and methods
Key instrument equipment and reagent: eppendorf 5702 centrifuges, centrifuge tube, EDTA heparin tube, syringe, liquaemin, Automatic Blood Cell Analyzer.
S2. experimental subjects: first filter out patient according to following 6:
1, intend accepting voluntarily the patient of platelet rich plasma injection for curing.
2, Venous Blood blood cell analysis result three ties up in normal range (NR): platelet count is 100～300 * 10
9between/L, hemoglobin women is between 135～150g/L, and the male sex is between 140～155g/L, and leukocyte count is 4～10 * 10
12between/L.
3, erythrocyte sedimentation rate (ESR), c reactive protein are in normal range (NR).
4, whole body and treatment are local without infecting shower.
5, base case is good, and ahyperlipoidemia, diabetes etc. affect the outer disease of huge hematological system to blood constituent.
6, patient's informed consent.
S3. experimental technique
S31. group technology: first 17 kinds of centrifugal treating methods are numbered (1 to No. 17), then allow volunteer select a number, according to the corresponding centrifugal treating method of number, enter group, as volunteer 1 has selected number 1, be dispensed to 1 group, its corresponding centrifugal treating method is the centrifugal treating method of numbering 1.17 kinds of centrifugal treating methods are as follows: through above-mentioned conditional filtering, go out to test after volunteer, with heparin moistening syringe in patient's ulnar vein, gather blood 50mL, inject the processing scheme of centrifuge tube Nei，An Qi place group and carry out centrifugal treating:
17 kinds of centrifugal treating methods of table 1
In 1～12 group and 14～17 groups, the addition of heparin is that 0.2:10 determines according to the volume ratio of heparin and whole blood, and the addition of heparin is that 0.25:10 determines according to the volume ratio of heparin and whole blood in 13 groups.The concentration of described heparin is 12500 units/2mL.
S32. centrifugal method and check:
Whole blood routine inspection: gather 2mL blood with EDTA heparin tube in patient's ulnar vein, do whole blood conventional analysis.
With 1 of the moistening 60mL syringe of the heparin of using in advance 1.0mL12500 unit/2mL, in patient's ulnar vein, gather 50mL blood, after shaking up gently, inject 50mL centrifuge tube.With after an other centrifuge tube trim, according to the grouping scheme originally designing, carry out centrifugal.After centrifugal end, with minute hand Tou Yu middle level, evenly draw 4mL blood plasma (traditional Landesberg method is to draw 4mL blood plasma in bottom), inject EDTA heparin tube, do whole blood conventional analysis.
S4. statistical method: 3 times of comparisons of platelet count (PLT) and its whole blood platelet count in the platelet rich plasma preparing with centrifugal process of the present invention, adopt paired t-test; At above-mentioned comparing difference, there is the comparison between the group of statistical significance, adopt single factor scheme analysis or non-parametric test; After above twice check, draw the group that blood platelet is the highest, by itself and traditional Landesberg method comparison, relatively employing one-way analysis of variance or the non-parametric test of PLT and HGB in both PRP.
S5. result
S51. the platelet count in the platelet rich plasma that 17 kinds of centrifugal treating methods prepare, its descriptive statistics sees the following form: in the sample size of various centrifugal methods, the platelet rich plasma preparing, the standard deviation of blood platelet average, average, standard error ,95% credibility interval, maximum and minimizing statistic see the following form 2.
Platelet count statistics in the PRP that 17 kinds of centrifugal treating methods of table 2 prepare
S52. the hematoblastic histogram in the platelet rich plasma that various centrifugal methods prepare in blood platelet and its whole blood is shown in Fig. 1.Interpretation of result by upper table 2 and Fig. 1 is known: in 17 groups an of centrifugal process, the group that platelet rich plasma average is the highest is 15min-1400g group, and its average is 1029.875 * 10
9/ L, standard deviation is 259.916, it is (812.5796,1247.1704) that standard is mistaken for 91.894,95% confidential intervals.In the situation that increasing heparin consumption, the blood platelet average in 15min-1400g group platelet rich plasma is 726.55, shows to increase heparin and can cause that the blood platelet in platelet rich plasma declines.Under the centrifugation time condition of definite 15 minutes, best centrifugal force is 1400g, increases or reduces centrifugal force and all cause blood platelet in platelet rich plasma to decline.
S53. in whole blood and platelet rich plasma, the comparison (SPSS17.0, paired t-test) of platelet count the results are shown in Table 3 and Fig. 2 in 17 groups.
Interpretation of result by table 3 and Fig. 2 is known: in the platelet rich plasma preparing, platelet count is that in whole blood, 3 times of above groups of platelet count have 6 minutes 200g groups, 6 minutes 1000g groups, 6 minutes 1400g groups, 6 minutes 1800g groups, 10 minutes 1900g groups, 15 minutes 1200g groups, 15 minutes 1300g groups, 15 minutes 1400g groups, 15 minutes 3000g and increase heparin consumption group.Wherein in blood platelet blood plasma in platelet count and whole blood 3 times of platelet count relatively to have the group of statistical significance be 15 minutes 1400g groups, P < 0.01.Therefore, can think, while preparing PRP with a centrifugal process, adopt 1400g centrifugal force to carry out just preparing high-quality PRP in centrifugal 15 minutes, this is the technical method of patent of the present invention.
The comparative result of platelet count in 17 groups of interior whole bloods of table 3 and platelet rich plasma
S55. utilize traditional standby PRP of secondary centrifuging method-Landesberg legal system that in the PRP that the method for the invention (15min-1400g centrifugal force) prepares, the average Yu Ben research unit of PLT and hemoglobin content adopts to compare, the results are shown in following table 4 and table 5.
Table 4
Table 5
From upper table 4 and table 5, once during centrifugal preparation PRP, in the PRP that the technical method of patent of the present invention of take prepares, platelet count is 1029.875 * 10
9/ L, with 1204.833 * 10 of Landesberg method
9/ L compares, and adopts the t check of two independent samples, t=-0.919, and P=0.382, difference does not have statistical significance, can think that the technical method of patent of the present invention can be prepared and the hematoblastic PRP of the same high concentration of secondary centrifuging method Landesberg method.
S56. adopt technical scheme of the present invention (15 minutes-1400g centrifugal force) and Landesberg legal system for the erythrocyte in PRP (HGB) comparative result see the following form 6, table 7 and Fig. 3.
Table 6
Table 7
Known by table 6 and table 7: in the PRP that the technical scheme of this patent invention and Landesberg method prepare, HGB average is respectively 20.2625g/L and 59.533/L, both relatively, difference has statistical significance, can think that content of hemoglobin is low compared with Landesberg method in the PRP preparing with patent of the present invention.(two independent sample t checks, t=-4.092, P=0.003)
As shown in Figure 3, while preparing PRP with a centrifugal process, the technical method of patent of the present invention (15min-1400g) is a kind of best preparation method.It is compared with Landesberg method, in the PRP preparing, platelet count does not have difference, but in the PRP that the technical scheme of patent of the present invention of take prepares, hemoglobin content is but low than Landesberg method (HGB is 59.533g/L), therefore can think, the technical scheme of patent of the present invention can be prepared into draws an analogy the more PRP of high-quality of Landesberg method.
Claims (5)
- One kind once centrifugal process prepare the method for platelet rich plasma, it is characterized in that, by the whole blood that is mixed with anti-coagulants with the centrifugal force of 1400g centrifugal 15 minutes, after centrifugal end, it is that platelet poor plasma Ceng， middle level is platelet rich plasma layer that whole blood will be divided into 3 Ceng， upper stratas, lower floor is hemoglobin layer, then evenly draws middle level blood plasma and obtains platelet rich plasma.
- 2. prepare according to claim 1 the method for platelet rich plasma, it is characterized in that, described anti-coagulants is heparin or hirudin.
- 3. prepare according to claim 1 the method for platelet rich plasma, it is characterized in that, described anti-coagulants is heparin.
- 4. prepare according to claim 3 the method for platelet rich plasma, it is characterized in that, the addition of described heparin in whole blood is that 0.2:10 determines according to the volume ratio of heparin and whole blood, the heparin that described heparin is 12500 units/2mL.
- 5. prepare according to claim 1 the method for platelet rich plasma, it is characterized in that, the concrete preparation method of described platelet rich plasma is: by being mixed with 1.0mL concentration, be that the 50mL whole blood of 12500 units/2mL heparin carries out centrifugal 15min minute with 1400g centrifugal force; After centrifugal end, it is that platelet poor plasma Ceng， middle level is that platelet rich plasma Ceng， lower floor is that hemoglobin Ceng，Yu middle level is evenly drawn 4mL blood plasma and obtained platelet rich plasma that whole blood will be divided into 3 Ceng， upper stratas.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105498000A (en) * | 2016-01-20 | 2016-04-20 | 廊坊市中心血站 | Method-plasma quality control chart for ensuring that blood quality meets blood transfusion effect |
| CN106669875A (en) * | 2017-02-13 | 2017-05-17 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| CN107075478A (en) * | 2014-07-23 | 2017-08-18 | 塞鲁斯公司 | The method for preparing platelet product |
| CN107617236A (en) * | 2017-09-28 | 2018-01-23 | 安徽信灵检验医学科技有限公司 | A kind of platelet rich liquid |
| CN108654141A (en) * | 2018-06-08 | 2018-10-16 | 宁波华科润生物科技有限公司 | A kind of platelet rich plasma preparation facilities |
| CN111420813A (en) * | 2020-04-06 | 2020-07-17 | 四川大学华西医院 | PRP centrifugal sleeve, PRP centrifugal machine and PRP preparation method |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020104808A1 (en) * | 2000-06-30 | 2002-08-08 | Lou Blasetti | Method and apparatus for producing platelet rich plasma and/or platelet concentrate |
| US20030094425A1 (en) * | 2001-11-16 | 2003-05-22 | Robert Brandt | Blood processing system and composition |
| US20120183519A1 (en) * | 2011-01-13 | 2012-07-19 | Biomet Biologics, Llc | Treatment of erectile dysfunction using platelet-rich plasma |
| CN102755770A (en) * | 2012-07-30 | 2012-10-31 | 博雅干细胞科技有限公司 | Extraction method of platelet rich plasma (PRP) and extracted PRP |
| CN102940912A (en) * | 2012-10-16 | 2013-02-27 | 丁志伟 | Platelet-rich plasma and bone powder collecting and separating device in operation |
| CN103071191A (en) * | 2013-02-04 | 2013-05-01 | 成都清科生物科技有限公司 | Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation |
-
2013
- 2013-10-15 CN CN201310480780.XA patent/CN103505910A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020104808A1 (en) * | 2000-06-30 | 2002-08-08 | Lou Blasetti | Method and apparatus for producing platelet rich plasma and/or platelet concentrate |
| US20030094425A1 (en) * | 2001-11-16 | 2003-05-22 | Robert Brandt | Blood processing system and composition |
| US20120183519A1 (en) * | 2011-01-13 | 2012-07-19 | Biomet Biologics, Llc | Treatment of erectile dysfunction using platelet-rich plasma |
| CN102755770A (en) * | 2012-07-30 | 2012-10-31 | 博雅干细胞科技有限公司 | Extraction method of platelet rich plasma (PRP) and extracted PRP |
| CN102940912A (en) * | 2012-10-16 | 2013-02-27 | 丁志伟 | Platelet-rich plasma and bone powder collecting and separating device in operation |
| CN103071191A (en) * | 2013-02-04 | 2013-05-01 | 成都清科生物科技有限公司 | Preparation method of autologous platelet-factor-rich plasma (PFRP) preparation |
Cited By (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107075478A (en) * | 2014-07-23 | 2017-08-18 | 塞鲁斯公司 | The method for preparing platelet product |
| US10842818B2 (en) | 2014-07-23 | 2020-11-24 | Cerus Corporation | Methods for preparing platelet products |
| CN107075478B (en) * | 2014-07-23 | 2021-11-09 | 塞鲁斯公司 | Method for preparing platelet product |
| CN105498000A (en) * | 2016-01-20 | 2016-04-20 | 廊坊市中心血站 | Method-plasma quality control chart for ensuring that blood quality meets blood transfusion effect |
| CN105498000B (en) * | 2016-01-20 | 2017-11-07 | 廊坊市中心血站 | It is a kind of to ensure the method blood plasma Quality Control figure that blood quality meets blood transfusion effect |
| CN106669875A (en) * | 2017-02-13 | 2017-05-17 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| CN106669875B (en) * | 2017-02-13 | 2022-07-01 | 深圳市邦沃科技有限公司 | Platelet-rich plasma centrifuge tube and extraction method thereof |
| CN107617236A (en) * | 2017-09-28 | 2018-01-23 | 安徽信灵检验医学科技有限公司 | A kind of platelet rich liquid |
| CN108654141A (en) * | 2018-06-08 | 2018-10-16 | 宁波华科润生物科技有限公司 | A kind of platelet rich plasma preparation facilities |
| CN108654141B (en) * | 2018-06-08 | 2020-08-14 | 宁波华科润生物科技有限公司 | Platelet-rich plasma preparation device |
| CN111420813A (en) * | 2020-04-06 | 2020-07-17 | 四川大学华西医院 | PRP centrifugal sleeve, PRP centrifugal machine and PRP preparation method |
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Effective date of registration: 20160425 Address after: 510665, D, building 101, Tai Sheng Industrial Zone, Hollywood Road, Guangzhou, Guangdong, Tianhe District Applicant after: Guangdong Will Industry Co., Ltd. Address before: 510080 Zhongshan Road, Guangdong, No. two, No. 106, Guangzhou Applicant before: Guangdong General Hospital |
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Application publication date: 20140115 |