CN101726582A - Filtering method of procoagulant type test tube sample - Google Patents
Filtering method of procoagulant type test tube sample Download PDFInfo
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- CN101726582A CN101726582A CN200910216146A CN200910216146A CN101726582A CN 101726582 A CN101726582 A CN 101726582A CN 200910216146 A CN200910216146 A CN 200910216146A CN 200910216146 A CN200910216146 A CN 200910216146A CN 101726582 A CN101726582 A CN 101726582A
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Abstract
The invention provides a method for filtering educts after a sample in a procoagulant type test tube is subjected to a centrifugal action. The technical scheme is realized by comprising the following steps of: a. collecting blood into the procoagulant type test tube by using a vacuum blood taking technology, wherein the procoagulant type test tube is internally provided with a filter screen; and b. enabling the procoagulant type test tube to be subjected to the centrifugal action so that a solution in the procoagulant type test tube forms a zone belt with different levels, wherein a blood corpuscle layer and a blood serum layer are positioned in the procoagulant type test tube from bottom to top, and the filter screen sinks on the blood corpuscle layer along a tube wall under the action of centrifugal force. The invention can effectively filter out floating fiber protein filaments and separation gel educts in the blood serum obtained after centrifugation, reduces the pollution of the fiber protein filaments and the separation gel educts on a sampling probe and a colorimetric cuvette, and ensures the detection result of a blood sample to be more accurate.
Description
Technical field:
The invention provides a kind of interior sample of procoagulant type test tube that makes after centrifugation, can filter the wherein method of precipitate.
Background technology:
At present, the procoagulant type test tube on the market comprises short pipe, common tube with fixed attention and separates sebific duct, three kinds of test tube purposes unanimities.Short coagulating in the pipe is added with set accelerator, accelerates blood preparation and solidifies; Do not have set accelerator in the common tube, blood preparation solidifies slower relatively; Separate sebific duct and be added with set accelerator and separation gel simultaneously, fast separation of serum.
Procoagulant type test tube is after blood drawing is centrifugal, and more or less all floating in the serum have its main cause of precipitate of fiber protein yarn and separation gel to have:
1. as adopt short pipe or the common tube of coagulating to draw blood centrifugally, inside pipe wall is when carrying out surface treatment, because individual difference, indivedual test tubes can glue blood, centrifugal after, fiber protein yarn can not precipitate down fully attached to tube wall, so have fiber protein yarn in the serum;
2. as adopting the separation sebific duct to draw blood centrifugal, separation gel composition wherein is the polyolefins macromolecule resin, poly-halogenated hydrocarbons macromolecular compound and mineral compound filler, because separation gel is a mixed system, different materials density wherein is all different, in processing mixed process, as mix inequality and promptly have precipitate and float in the serum, its precipitate is mainly micromolecule micelle and oil.
Procoagulant type test tube is through after centrifugal, and test tube places on the full automatic biochemical apparatus, and the sampling probe on the full automatic biochemical apparatus can put in automatically and draw serum in the heparin tube, in the cuvette that reinjects, adds reagent and carries out colorimetric.As fiber protein yarn and micromolecule micelle are arranged in the serum, sampling probe can stop up (the sampling probe internal diameter has only a few tenths of a mm) when sampling, can not inhale serum as stopping up sampling probe fully, and the result is zero.As half obstruction, sampling amount is not enough, and serum and ratio of reagents can change, so cause the result inaccurate; In the serum if any oil, sampling probe is with oily inspiration cuvette, oil can be adsorbed on the cuvette, and its cuvette transmittance of long-time back can reduce, and full automatic biochemical apparatus is by the light measurement solution absorbency with certain wavelength, calculate the result, descend as transmittance, can influence absorbance measurement, thereby influence testing result, cause the blood preparation testing result inaccurate, influence the diagnosis of doctor disease.
Short coagulate blood preparation in pipe and the common tube after centrifugal, haemocyte and serum is not owing to there is the obstruct of separation gel, behind the placement certain hour, red blood cell can dissolve, cause blood preparation haemolysis, when sample is rechecked, can influence testing result, be unfavorable for the long-time preservation of sample.
Summary of the invention:
In view of above present situation, the invention provides a kind of filtering method of procoagulant type test tube sample, solved the problem that above-mentioned classic method exists.
Technical scheme of the present invention realizes by following content:
A kind of filtering method of procoagulant type test tube sample is characterized in that: its steps in sequence is
A. utilize vacuum blood drawing technique with blood collecting in procoagulant type test tube, described procoagulant type test tube inside is provided with filter screen.
B. make procoagulant type test tube be subjected to action of centrifugal force, the solution in the procoagulant type test tube forms district's band of different levels, is respectively haemocyte layer, serum layer in the procoagulant type test tube from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Described procoagulant type test tube is the short pipe that coagulates, short coagulating in the pipe has set accelerator, described filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than the diameter of haemocyte, and serum is liquid, and centrifugal back serum can pass through the screen pack aperture, and haemocyte can not pass through filter screen, the filter screen diameter is greater than the procoagulant type test tube internal diameter, and the centrifugal force size is RCF1800-2600g, and the centrifugal time is 10-12 minute.
Described procoagulant type test tube is a common tube, described filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than the diameter of haemocyte, serum is liquid, centrifugal back serum can pass through the screen pack aperture, and haemocyte can not pass through filter screen, and the filter screen diameter is greater than the procoagulant type test tube internal diameter, the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
Described procoagulant type test tube is for separating sebific duct, separate in the sebific duct separation gel is arranged, described filter screen is the unidirectional filter screen of macromolecule, and the filter screen mesh aperture is less than the diameter of haemocyte, and less than the diameter of micelle in the separation gel, serum is liquid, centrifugal back serum can pass through the screen pack aperture, and the micelle of haemocyte and separation gel can not pass through filter screen, and the filter screen diameter is greater than the procoagulant type test tube internal diameter, the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
In the described separation sebific duct set accelerator is arranged.
Advantage of the present invention:
1, uses the inventive method can effectively filter out floating fiber protein yarn and separation gel precipitate in the serum that obtains after centrifugal, reduce its pollution, make the blood preparation testing result more accurate sampling probe and cuvette.
2, as adopting short pipe or the common tube of coagulating, haemocyte after centrifugal and serum is not owing to there is the obstruct of separation gel, but between haemocyte and serum filter screen arranged, and red blood cell can be dissolved in the serum, causes haemolysis, helps long-time preservation.
Description of drawings:
Fig. 1 is common tube blood sampling-centrifugation step synoptic diagram;
Fig. 2 is the short blood sampling tube-centrifugation step synoptic diagram that coagulates;
Fig. 3 is separation gel blood sampling tube-centrifugation step synoptic diagram;
1, procoagulant type test tube 2, filter screen 3, blood 4, serum layer 5, haemocyte layer 6, separation glue-line 7, set accelerator
Embodiment:
A kind of filtering method of procoagulant type test tube sample, its steps in sequence be a. utilize vacuum blood drawing technique with blood collecting in procoagulant type test tube, described procoagulant type test tube inside is provided with filter screen.B. make procoagulant type test tube be subjected to action of centrifugal force, the solution in the procoagulant type test tube forms district's band of different levels, is respectively haemocyte layer, serum layer in the procoagulant type test tube from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Procoagulant type test tube is the short pipe that coagulates, short coagulating in the pipe has set accelerator, described filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than the diameter of haemocyte, and serum is liquid, and centrifugal back serum layer can pass through the screen pack aperture, and haemocyte can not pass through filter screen, the filter screen diameter is greater than the procoagulant type test tube internal diameter, and the centrifugal force size is RCF1800-2600g, and the centrifugal time is 10-12 minute.
Procoagulant type test tube is a common tube, described filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than the diameter of haemocyte, serum is liquid, centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen, and the filter screen diameter is greater than the procoagulant type test tube internal diameter, the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
Procoagulant type test tube is for separating sebific duct, separate in the sebific duct separation glue-line is arranged, described filter screen is the unidirectional filter screen of macromolecule, the filter screen mesh aperture is less than the diameter of haemocyte, and diameter less than micelle in the separation gel, serum is liquid, centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen with the micelle that separates glue-line, separate glue-line and be positioned on the haemocyte layer, below the filter screen, the filter screen diameter is greater than the procoagulant type test tube internal diameter, the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
Separate in the sebific duct set accelerator is arranged.
A kind of filtering method of procoagulant type test tube sample, its steps in sequence are that a. utilizes vacuum blood drawing technique that blood collecting is coagulated in the pipe in short, urge to manage inside with fixed attention and are provided with filter screen.B. make the short pipe that coagulates be subjected to action of centrifugal force, the short solution that coagulates in the pipe pipe forms district's band of different levels, and short coagulating in the pipe is respectively haemocyte layer, serum layer from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Short coagulating in the pipe has set accelerator, filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, and serum is liquid, and centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen, the filter screen diameter is greater than the short bore that coagulates, and the centrifugal force size is RCF 1800g, and the centrifugal time is 12 minutes.Set accelerator attached to the tube wall and the pipe end, does not influence the specimen examination result in centrifugal back.
A kind of filtering method of procoagulant type test tube sample, its steps in sequence are that a. utilizes vacuum blood drawing technique that blood collecting is coagulated in the pipe in short, urge to manage inside with fixed attention and are provided with filter screen.B. make the short pipe that coagulates be subjected to action of centrifugal force, the short solution that coagulates in the pipe pipe forms district's band of different levels, and short coagulating in the pipe is respectively haemocyte layer, serum layer from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Short coagulating in the pipe has set accelerator, filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, and serum is liquid, and centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen, the filter screen diameter is greater than the short bore that coagulates, and the centrifugal force size is RCF 2600g, and the centrifugal time is 10 minutes.Set accelerator attached to the tube wall and the pipe end, does not influence the specimen examination result in centrifugal back.
A kind of filtering method of procoagulant type test tube sample, its steps in sequence be a. utilize vacuum blood drawing technique with blood collecting in common tube, common tube inside is provided with filter screen.B. make common tube be subjected to action of centrifugal force, the solution in the common tube forms district's band of different levels, is respectively haemocyte layer, serum layer in the common tube from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Filter screen is the unidirectional filter screen of macromolecule in the common tube, the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, serum is liquid, centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen, and the filter screen diameter is greater than the short bore that coagulates, the centrifugal force size is RCF 2000g, and the centrifugal time is 11 minutes.
Embodiment 4
A kind of filtering method of procoagulant type test tube sample, its steps in sequence be a. utilize vacuum blood drawing technique with blood collecting in common tube, common tube inside is provided with filter screen.B. make common tube be subjected to action of centrifugal force, the solution in the common tube forms district's band of different levels, is respectively haemocyte layer, serum layer in the common tube from the bottom to top, and wherein filter screen sinks down on the haemocyte layer at action of centrifugal force lower edge tube wall.
Filter screen is the unidirectional filter screen of macromolecule in the common tube, the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, serum is liquid, centrifugal back serum layer can pass through the screen pack aperture, and the haemocyte layer can not pass through filter screen, and the filter screen diameter is greater than the short bore that coagulates, the centrifugal force size is RCF2400g, and the centrifugal time is 12 minutes.
A kind of filtering method of procoagulant type test tube sample, its steps in sequence be a. utilize vacuum blood drawing technique with blood collecting in separating sebific duct, separate sebific duct inside and be provided with filter screen.B. make the separation sebific duct be subjected to action of centrifugal force, the district's band that separates the solution formation different levels in the sebific duct pipe, separate being respectively haemocyte layer, separation glue-line in the sebific duct from the bottom to top, the serum layer, wherein filter screen sinks down at action of centrifugal force lower edge tube wall and separates on the glue-line.
Separating in the sebific duct has the separation glue-line, and filter screen is the unidirectional filter screen of macromolecule, and the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, and less than the diameter of micelle in the separation gel, serum is liquid, and centrifugal back serum layer can pass through the filter screen mesh, and the haemocyte layer can not pass through filter screen with the micelle that separates glue-line, separate glue-line and be positioned on the haemocyte layer, under the filter screen, the filter screen diameter is greater than the separation gel bore, the centrifugal force size is RCF 1800g, and the centrifugal time is 12 minutes.
A kind of filtering method of procoagulant type test tube sample, its steps in sequence be a. utilize vacuum blood drawing technique with blood collecting in separating sebific duct, separate sebific duct inside and be provided with filter screen.B. make the separation sebific duct be subjected to action of centrifugal force, the district's band that separates the solution formation different levels in the sebific duct pipe, separate being respectively haemocyte layer, separation glue-line, serum layer in the sebific duct from the bottom to top, wherein filter screen sinks down at action of centrifugal force lower edge tube wall and separates on the glue-line.
Separate the glue-line of separation and set accelerator are arranged in the sebific duct, filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen is less than 5 microns, the diameter of haemocyte is generally more than 7 microns, and diameter less than micelle in the separation gel, serum is liquid, centrifugal back serum layer can pass through the screen pack mesh, and the haemocyte layer can not pass through filter screen with the micelle that separates glue-line, separate glue-line and be positioned on the haemocyte layer, under the filter screen, the filter screen diameter is greater than the separation gel bore, the centrifugal force size is RCF 2600g, and the centrifugal time is 10 minutes.Set accelerator in the separation sebific duct attached to the tube wall and the pipe end, does not influence the specimen examination result in centrifugal back.
Claims (5)
1. filtering method of procoagulant type test tube sample, it is characterized in that: its steps in sequence is
A. utilize vacuum blood drawing technique that blood (3) is collected in the procoagulant type test tube (1), described procoagulant type test tube (1) inside is provided with filter screen (2).
B. make procoagulant type test tube (1) be subjected to action of centrifugal force, solution in the procoagulant type test tube forms district's band of different levels, be respectively haemocyte layer (5), serum layer (4) in the procoagulant type test tube from the bottom to top, wherein filter screen (2) sinks down on the haemocyte layer (5) at action of centrifugal force lower edge tube wall.
2. filtering method of procoagulant type test tube sample according to claim 1, it is characterized in that: described procoagulant type test tube (1) is the short pipe that coagulates, short coagulating in the pipe has set accelerator (7), described filter screen (2) is the unidirectional filter screen of macromolecule, the aperture of filter screen (2) is less than the diameter of haemocyte, serum is liquid, centrifugal back serum layer (4) can be by the screen pack aperture, and haemocyte layer (5) can not pass through filter screen, the filter screen diameter is greater than the procoagulant type test tube internal diameter, the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
3. filtering method of procoagulant type test tube sample according to claim 1, it is characterized in that: described procoagulant type test tube (1) is common tube, described filter screen is the unidirectional filter screen of macromolecule, the aperture of filter screen (2) is less than the diameter of haemocyte, and serum is liquid, and centrifugal back serum layer (4) can be by the screen pack aperture, and haemocyte layer (5) can not pass through filter screen, filter screen (2) diameter is greater than the procoagulant type test tube internal diameter, and the centrifugal force size is RCF1800-2600g, and the centrifugal time is 10-12 minute.
4. filtering method of procoagulant type test tube sample according to claim 1, it is characterized in that: described procoagulant type test tube (1) is for separating sebific duct, separate in the sebific duct separation glue-line (6) is arranged, described filter screen (2) is the unidirectional filter screen of macromolecule, the filter screen mesh aperture is less than the diameter of haemocyte, and less than the diameter that separates micelle in the glue-line (6), serum is liquid, centrifugal back serum layer (4) can be by the screen pack aperture, and haemocyte layer (5) can not pass through filter screen with the micelle that separates glue-line (6), the filter screen diameter is greater than the procoagulant type test tube internal diameter, and the centrifugal force size is RCF 1800-2600g, and the centrifugal time is 10-12 minute.
5. filtering method of procoagulant type test tube sample according to claim 4 is characterized in that: set accelerator (7) is arranged in the described separation sebific duct.
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| CN200910216146A CN101726582A (en) | 2009-11-05 | 2009-11-05 | Filtering method of procoagulant type test tube sample |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669281A (en) * | 2017-11-02 | 2018-02-09 | 犹银俊 | Animal blood serum gathers test tube and serum separator |
| CN109283325A (en) * | 2018-05-04 | 2019-01-29 | 遵义医学院附属医院 | Detect fiber filament blood, grumeleuse oligemia and chylemia processing method and utensil |
| CN113005177A (en) * | 2021-03-24 | 2021-06-22 | 杭州倍强医药科技有限公司 | Blood sample obtaining method, blood sample and application |
-
2009
- 2009-11-05 CN CN200910216146A patent/CN101726582A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107669281A (en) * | 2017-11-02 | 2018-02-09 | 犹银俊 | Animal blood serum gathers test tube and serum separator |
| CN109283325A (en) * | 2018-05-04 | 2019-01-29 | 遵义医学院附属医院 | Detect fiber filament blood, grumeleuse oligemia and chylemia processing method and utensil |
| CN113005177A (en) * | 2021-03-24 | 2021-06-22 | 杭州倍强医药科技有限公司 | Blood sample obtaining method, blood sample and application |
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Application publication date: 20100609 |