CN111420813A - PRP centrifugal sleeve, PRP centrifugal machine and PRP preparation method - Google Patents
PRP centrifugal sleeve, PRP centrifugal machine and PRP preparation method Download PDFInfo
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- CN111420813A CN111420813A CN202010262345.XA CN202010262345A CN111420813A CN 111420813 A CN111420813 A CN 111420813A CN 202010262345 A CN202010262345 A CN 202010262345A CN 111420813 A CN111420813 A CN 111420813A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B15/00—Other accessories for centrifuges
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B04—CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
- B04B—CENTRIFUGES
- B04B5/00—Other centrifuges
- B04B5/04—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
- B04B5/0407—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
- B04B5/0414—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes
- B04B5/0421—Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles comprising test tubes pivotably mounted
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Abstract
The invention provides a PRP centrifugal sleeve, a PRP centrifugal machine and a PRP preparation method, relates to the technical field of PRP preparation, and solves the problems that the time for preparing PRP is long and infection risk exists in secondary centrifugation; the helical coil is spirally wound around the axis of the outer sleeve and is wound around the periphery of the blood collection tube arranged in the outer sleeve. A sleeve frame is fixed on a rotating shaft of a centrifuge main body of the PRP centrifuge, and an outer sleeve is arranged on the sleeve frame; the pulse current generator is arranged on the centrifuge body and is electrically connected with the spiral coil. According to the PRP preparation method, pulse alternating current is introduced into the spiral coil during centrifugation, so that the magnetic field with the direction changed by the spiral coil acts on the charged growth factors in the blood collection tube, the preparation time of the PRP can be shortened, and the trouble and pollution risk of secondary centrifugation operation are avoided.
Description
Technical Field
The invention relates to the technical field of PRP preparation, in particular to a PRP centrifugal sleeve for preparing PRP, a PRP centrifugal machine provided with the PRP centrifugal sleeve and a PRP preparation method using the PRP centrifugal machine.
Background
PRP (Platelet-rich plasma) is a plasma component obtained by centrifuging and extracting autologous blood and containing a large amount of platelets, which are significantly higher than the level of platelets in normal human plasma.
The two-time centrifugation generally first passes through a relatively low speed centrifugation (speed 100 × G, centrifugation 10min) to separate plasma and platelets from red blood cells and white blood cells, and then passes through a relatively high speed centrifugation (centrifugal force 400 × G, centrifugation 10min) to further concentrate PRP and separate platelet poor plasma components to obtain an optimal platelet concentration, Marx et al use 2 centrifugation speeds of 5600rpm, 2400rpm, average platelet concentration of 3.3G/L, Perez et al use 1 centrifugation speed of 723rpm for 10min, 2 centrifugation speed of 1445rpm for 10min, platelet concentration 3 times higher than the base concentration, G mez L A et al propose a single centrifugation speed of 1500rpm for 10min to obtain a baseline value with platelet concentration 1.6-4.9 times higher than the base concentration, while reducing the handling of the sample to reduce the risk of PRP contamination and platelet loss.
The current PRP needs secondary centrifugation to ensure effective concentration, takes a lot of time to wait for preparation, and has a high risk of secondary centrifugation infection.
The growth factors have positive charge and negative charge, and can be interfered by a magnetic field. For example, platelet derived growth factor (PDGF platelet derived growth factor) is a thermostable, highly positively charged protein consisting of a disulfide-bond containing dimer with a molecular weight of about 30000 daltons. For another example, Epidermal Growth Factor (EGF) is a thermostable polypeptide containing 53 amino acid residues and having a molecular weight of about 6000 daltons. aFGF is negatively charged in acidic environment. Therefore, how to introduce an electromagnetic field to act on charged growth factors in the preparation process of the PRP, and guiding the growth factors to be gathered to the white membrane layer by virtue of the pulsed electromagnetic field becomes a key for reducing the preparation time of the PRP and avoiding secondary centrifugal infection.
Disclosure of Invention
The invention aims to design a PRP centrifugal sleeve, a PRP centrifugal machine and a PRP preparation method, which can reduce the preparation time of PRP and avoid the trouble and pollution risk of secondary centrifugal operation.
The invention is realized by the following technical scheme:
the invention provides a PRP centrifugal sleeve, which comprises an outer sleeve and a spiral coil;
the outer sleeve is used for being connected with a rotating shaft of the centrifuge, and the spiral coil and the blood collection tube are arranged in the outer sleeve;
the spiral coil is arranged on the inner side of the outer sleeve, and two connecting ends of the spiral coil are used for being connected with a power supply outside the outer sleeve;
the spiral coil winds around the axis of the outer sleeve in a spiral mode, and the spiral coil is used for being wound on the periphery of a blood collection tube arranged in the outer sleeve in a winding mode.
When the structure is adopted, the PRP centrifugal sleeve with the spiral coil is fixedly arranged in the PRP centrifugal machine when in use, current such as pulse alternating current is introduced into the spiral coil during centrifugation, the spiral coil generates a magnetic field with constantly changing direction to act on the charged growth factors, the charged growth factors are promoted to gather towards a white film layer, the PRP preparation speed is promoted, the PRP with sufficient concentration can be obtained through single centrifugation, the PRP with high concentration is quickly obtained, and the preparation time and the infection risk are reduced.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the PRP centrifugal sleeve further comprises an inner sleeve for inserting a blood collection tube;
the inner sleeve is inserted into the inner side of the outer sleeve, and the spiral coil is wound on the periphery of the inner sleeve and the periphery of the blood sampling tube inserted into the inner sleeve.
When the structure is arranged, the spiral coil is arranged between the outer sleeve and the inner sleeve due to the arrangement of the inner sleeve, so that the collision of the inserted blood sampling tube on the spiral coil can be avoided, or the excessive deformation of the spiral coil in the centrifugal process can be avoided.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the PRP centrifugal sleeve further comprises a sleeve cover, and the sleeve cover covers a sleeve opening of the inner sleeve.
When the structure is adopted, the sleeve cover can better fix and protect the blood sampling tube in the sleeve.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the cover barrel mouth of outer sleeve is provided with the external screw thread, the cover barrel lid is provided with the internal thread, the cover barrel lid with outer sleeve threaded connection.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the outer sleeve is fixedly connected or detachably connected with the inner sleeve.
When the structure is adopted, the outer sleeve and the inner sleeve are detachably connected, so that the spiral coil in the outer sleeve and the inner sleeve can be conveniently replaced or maintained.
The invention also provides a PRP centrifugal machine, which comprises a centrifugal machine main body and the PRP centrifugal sleeve;
the centrifuge body comprises a sleeve frame and a pulse current generator; the sleeve frame is fixed on a rotating shaft of the centrifuge body, and the outer sleeve is mounted on the sleeve frame; the pulse current generator is installed in the centrifuge body, and the pulse current generator is electrically connected with the two connecting ends of the spiral coil through electric wires.
When the structure is adopted, pulse alternating current is introduced into the spiral coil during centrifugation, the spiral coil generates a magnetic field with constantly changing direction to act on the charged growth factors, the charged growth factors are promoted to be gathered to the white membrane layer, the preparation speed of PRP is promoted, PRP with enough concentration can be obtained through single centrifugation, the PRP with high concentration can be obtained quickly, and the preparation time and the infection risk are reduced.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the sleeve frame comprises an upper connecting arm and a lower connecting arm arranged below the upper connecting arm; the upper connecting arm is connected with the part, close to the pipe orifice, of the outer sleeve, and the lower connecting arm is connected with the part, close to the pipe tail, of the outer sleeve.
When the structure is adopted, the upper and lower double-layer connecting arms can be connected with the outer sleeve more stably.
In order to further better implement the invention, the following arrangement structure is particularly adopted: the last linking arm of telescopic frame is at least two, all go up the linking arm and wind the contained angle such as pivot of centrifuge main part is arranged, the linking arm is unanimous and corresponds the setting from top to bottom with the quantity of last linking arm down, and is every right go up the linking arm with one is connected to lower linking arm the outer sleeve.
When the structure is arranged, a plurality of blood sampling tubes can be centrifuged at the same time.
In order to further better implement the invention, the following arrangement structure is particularly adopted: each upper connecting arm is provided with an upper electric wire, and two ends of each upper electric wire are respectively and electrically connected with one connecting end of the pulse current generator and one connecting end of the spiral coil; and the lower connecting arm is provided with a lower electric wire, and two ends of the lower electric wire are respectively and electrically connected with the pulse current generator and the other connecting end of the spiral coil.
When adopting above-mentioned structure that sets up, top electric wire and below electric wire can be by the outrigger through arranging at last linking arm and linking arm down, are connected with two links of spiral coil simultaneously, can make the interior circuit layout of centrifuge body more regular and reduce the influence of rotating part to the electric wire.
The invention also provides a PRP preparation method, which uses the PRP centrifugal machine to prepare concentrated PRP and separate platelet poor plasma components, and comprises the following steps:
s1: collecting venous blood in a blood collection tube under aseptic conditions;
s2: placing the blood collection tube with the collected venous blood into a PRP centrifugal sleeve;
s3: setting centrifugal force and centrifugal time;
s4: and (3) introducing pulse alternating current into the spiral coil of the PRP centrifugal sleeve when the centrifugation is started, so that the magnetic field with the direction changed by the spiral coil acts on the charged growth factors in the blood collection tube, and the charged growth factors are promoted to be gathered towards the leucoderma layer to quickly obtain the PRP with high concentration.
When the method is adopted, during centrifugation, pulse alternating current is introduced into the spiral coil, the spiral coil generates a magnetic field with constantly changing direction to act on the charged growth factors, the charged growth factors are promoted to gather to the white membrane layer, so that the preparation speed of the PRP is promoted, the PRP with enough concentration can be obtained through single centrifugation, the PRP with high concentration can be quickly obtained, and the preparation time and the infection risk are reduced.
Further, the invention is better realized by the following steps: the centrifugal force is set to be 100-1500xg, the centrifugal time is set to be 5-10min, the output current voltage of the pulse current generator is 220V, and the frequency is 100-100 kHz.
The invention has the following advantages and beneficial effects:
in the invention, the PRP centrifugal sleeve with the spiral coil is arranged in a PRP centrifugal machine for fixing when in use, and the PRP centrifugal sleeve can also be arranged after the existing centrifugal machine is improved. During centrifugation, current, such as pulse alternating current, is introduced into the spiral coil, the spiral coil generates a magnetic field with constantly changing direction to act on the charged growth factors, the charged growth factors are promoted to gather towards the white membrane layer, so that the preparation speed of the PRP is promoted, the PRP with enough concentration can be obtained through single centrifugation, the PRP with high concentration can be obtained quickly, and the preparation time and the infection risk are reduced.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural view of a PRP centrifuge sleeve;
FIG. 2 is a schematic diagram of a PRP centrifuge;
FIG. 3 is a schematic view of a sleeve mount configuration;
FIG. 4 is a schematic diagram of the electrical connection of the spiral coil to the pulse current generator;
labeled as:
1. an outer sleeve; 2. a helical coil; 3. an inner sleeve; 4. a sleeve cover; 5. a centrifuge body; 6. a sleeve frame; 61. an upper connecting arm; 62. a lower connecting arm; 7. a pulse current generator; 81. an upper wire; 82. and a lower wire.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
Example 1:
the PRP centrifugal sleeve can reduce the preparation time of PRP and avoid the trouble and pollution risk of secondary centrifugal operation, and is particularly arranged into the following structures as shown in figures 1, 2, 3 and 4:
basically, the PRP centrifuge sleeve comprises an outer sleeve 1 and a helical coil 2. The top of the outer sleeve 1 is provided with an opening for accommodating the spiral coil 2 and the blood sampling tube, the bottom of the outer sleeve 1 is closed, the outer sleeve 1 can be directly or indirectly connected with a rotating shaft of the centrifuge, and the space in the outer sleeve 1 is used for arranging the spiral coil 2 and the blood sampling tube. The spiral coil 2 is arranged on the inner side of the outer sleeve 1, the spiral coil 2 is formed by spirally winding a conducting wire around the axis of the outer sleeve 1, the spiral coil 2 is used for being wound on the periphery of a blood sampling tube arranged in the outer sleeve 1, and two connecting ends of the spiral coil 2 extend out of a through hole formed in the surface of the outer sleeve 1 and are used for being connected with a power supply outside the outer sleeve 1.
The PRP centrifugal sleeve can be provided with a sleeve cap 4, and the sleeve cap 4 can be connected with the outer sleeve 1 by pressing or screwing to cover the inner spiral coil 2 and the blood sampling tube.
When the structure is adopted, the sleeve cover 4 can better fix and protect the blood sampling tube in the sleeve.
The PRP centrifugal sleeve with the spiral coil 2 is installed in a PRP centrifugal machine for fixing when in use, generally speaking, the existing centrifugal machine can be improved according to the PRP centrifugal sleeve, for example, a pulse current generator 7 is arranged in or outside the existing centrifugal machine, a conductive slip ring is installed in the centrifugal machine, an electric wire connected with a wiring port of the pulse current generator 7 is continuously connected with a section of electric wire in the centrifugal machine through the conductive slip ring, the continuously connected electric wire is arranged in a rotating shaft and penetrates out of the rotating shaft to be electrically connected with two connecting ends of the spiral coil, and during centrifugation, the spiral coil and the continuously connected electric wire arranged in the rotating shaft rotate along with the rotating shaft. Or a pulse current generator 7 which can rotate along with the rotating shaft can be arranged at the rotating shaft, the pulse current generator 7 is provided with a self-contained power supply or is powered by the power supply of the centrifugal machine, and the pulse current generator 7 is directly connected with the two connecting ends of the spiral coil 2 through a connecting terminal or indirectly electrically connected by utilizing the electromagnetic induction principle. During centrifugation, current is introduced into the spiral coil 2, for example, pulse alternating current output by the pulse current generator 7, the spiral coil 2 generates a magnetic field with constantly changing direction to act on charged growth factors in the blood collection tube, so that the charged growth factors are gathered to the white membrane layer, and the preparation speed of the PRP is accelerated.
Example 2:
the embodiment is further optimized on the basis of the above embodiment, and in order to further better implement the invention, the following arrangement structure is particularly adopted:
this PRP centrifugation sleeve still includes an inner skleeve 3, and inner skleeve 3's bottom is sealed, and inner skleeve 3's top has the opening, and the space of inner skleeve 3's inside is used for cartridge to go into the heparin tube, and the heparin tube can be fixed by inner skleeve 3 after packing into inner skleeve 3. The inner sleeve 3 is inserted into the inner side of the outer sleeve 1, the outer sleeve 1 and the inner sleeve 3 can be fixedly connected and are an integrally formed structural member, and can also be detachably connected and are a combined structural member formed by two independent parts. When the outer sleeve 1 and the inner sleeve 3 are combined structural members which are detachably connected, the spiral coil 2 in the outer sleeve and the inner sleeve can be conveniently replaced or maintained. The inner sleeve 3 and the outer sleeve 1 can be selected according to the actual situation. When the inner sleeve 3 is inserted into the outer sleeve 1, the spiral coil 2 is wound on the periphery of the inner sleeve 3, the axis of the spiral coil 2 is overlapped with the axes of the outer sleeve 1 and the inner sleeve 3, when the blood sampling tube is inserted into the inner sleeve 3, the spiral coil 2 is wound on the periphery of the blood sampling tube inserted into the inner sleeve 3, and the axis of the spiral coil 2 is overlapped with the axis of the blood sampling tube. The arrangement of the inner sleeve 3 enables the spiral coil 2 to be positioned between the outer sleeve 1 and the inner sleeve 3, so that the collision of the inserted blood sampling tube to the spiral coil 2 can be avoided, or the excessive deformation of the spiral coil 2 in the centrifugal process can be avoided.
The PRP centrifuge sleeve in this embodiment may also be provided with a sleeve cover 4. The sleeve mouth department of outer sleeve 1 forms the throat, and the outer wall department of throat is provided with the external screw thread, and the inboard of sleeve lid 4 is provided with the internal thread, and sleeve lid 4 and outer sleeve 1 threaded connection, during the connection, the sleeve mouth that inner sleeve 3 was established to sleeve lid 4 lid is used for including sealing the blood sampling tube in the inner sleeve 3, and sleeve lid 4 can be fixed better like this and protect the interior blood sampling tube of sleeve.
Example 3:
the present embodiment further provides a PRP centrifuge based on the above embodiment, which can reduce the preparation time of PRP, and avoid the trouble and pollution risk of secondary centrifugation operation, and particularly adopts the following arrangement structure:
the PRP centrifuge comprises a centrifuge body 5 and the PRP centrifuge sleeve of example 2.
The centrifuge main body 5 is internally provided with a pulse current generator 7, and a sleeve frame 6 is fixedly connected with the rotating shaft of the centrifuge main body 5. The sleeve frame 6 is fixed with a rotating shaft of the centrifuge main body 5 through a central shaft sleeve, the shaft sleeve of the sleeve frame 6 is provided with a connecting arm used for connecting the outer sleeve 1, and the outer sleeve 1 is installed at the connecting arm of the sleeve frame 6 to realize fixation.
The pulse current generator 7 is installed inside the centrifuge body 5. Can be equipped with two places in the centrifuge main part 5 and lead electrical slip ring, pulse current generator 7 is directly supplied power by centrifuge main part 5, and pulse current generator 7 is connected with two places and leads electrical slip ring through the electric wire, leads electrical slip ring and passes through binding post or utilize the electromagnetic induction principle to realize the electricity and connect with two links of helical coil 2 through the electric wire. The rotating shaft is a hollow shaft, and an electric wire connected with the conductive slip ring penetrates through the rotating shaft and then penetrates out of the rotating shaft to be connected with the spiral coil 2.
Preferably, for better fixing of the PRP centrifugal sleeve, the sleeve holder 6 comprises an upper connecting arm 61 and a lower connecting arm 62 arranged below the upper connecting arm 61; the upper connecting arm 61 and the lower connecting arm 62 are fixedly connected with the shaft sleeve, the upper connecting arm 61 is connected with the part, close to the pipe orifice, of the outer sleeve 1, the lower connecting arm 62 is connected with the part, close to the pipe tail, of the outer sleeve 1, and the upper connecting arm and the lower connecting arm can be connected with the outer sleeve 1 more firmly. Simultaneously, in order to carry out the centrifugation to a plurality of heparin tubes in the time of can, the last connecting arm 61 of telescopic frame 6 is at least two, for example four, and all last connecting arms 61 arrange into the cross connecting arm structure around the equal contained angles of the pivot of centrifuge main part 5, and lower connecting arm 62 corresponds the setting from top to bottom with the quantity unanimity of last connecting arm 61, and every outer sleeve 1 of connecting to last connecting arm 61 and lower connecting arm 62. Further, an upper electric wire 81 may be fixedly provided at each upper connecting arm 61, one end of the upper electric wire 81 is connected to one conductive slip ring to be electrically connected to the pulse current generator 7, and the other end of the upper electric wire 81 is electrically connected to one connection end of the spiral coil 2; each lower connecting arm 62 is fixedly provided with a lower electric wire 82, one end of the lower electric wire 82 is connected with another conductive slip ring and is electrically connected with the pulse current generator 7, the other end of the lower electric wire 82 is electrically connected with another connecting end of the spiral coil 2, the upper electric wire 81 and the lower electric wire 82 can be stably supported by arranging the upper connecting arm 61 and the lower connecting arm 62 and are simultaneously connected with the two connecting ends of the spiral coil 2, the circuit layout in the centrifuge body 5 can be more regular and the influence of the rotating part on the electric wires can be reduced.
When adopting above-mentioned structure that sets up, when the centrifugation, to spiral coil 2 lets in pulse alternating current, spiral coil 2 then produces the constantly changing magnetic field of direction and produces the effect to electrified growth factor, impels electrified growth factor to the white membrane layer gathering to promote PRP preparation speed, accessible single centrifugation obtains the PRP of sufficient concentration, thereby obtains the PRP of high concentration fast, has reduced preparation time and infection risk.
Example 4:
the embodiment further provides a PRP preparation method based on the above embodiment, which can reduce the preparation time of PRP and avoid the trouble and pollution risk of secondary centrifugation operation, and particularly adopts the following arrangement structure:
this PRP preparation method can be used to prepare concentrated PRP and separate platelet poor plasma fractions using the PRP centrifuge of example 3, comprising the steps of:
step S1: collecting venous blood in a blood collection tube under aseptic conditions;
step S2 is performed after step S1 is completed: placing the blood collection tube after collecting venous blood into an inner sleeve 3 of a PRP centrifugal sleeve for fixing, then covering a sleeve cover 4, and then covering an upper cover of a centrifuge;
after the step S2 is completed, step S3 is performed, in which the centrifugal force and the centrifugal time are set on the centrifuge, if the pulse current generator 7 can set the frequency, the centrifugal force is set to 1500-1500 × g, the centrifugal time is set to 5-10min, the output current voltage of the pulse current generator 7 is 220V, and the frequency is set to 100-100 kHz.
Step S4 is performed after step S3 is completed: when the centrifugation starts, the centrifuge leads pulse alternating current to the spiral coil 2 of the PRP centrifugation sleeve through the pulse current generator 7, so that the spiral coil generates a magnetic field with a direction changing to act on the charged growth factors in the blood collection tube, the charged growth factors are promoted to gather towards a leucoderma layer so as to concentrate PRP and separate platelet plasma components, the optimal platelet concentration is obtained, and the PRP with high concentration is rapidly obtained.
During centrifugation, pulse alternating current is introduced into the spiral coil 2, the spiral coil 2 generates a magnetic field with constantly changing direction to act on the charged growth factors, the charged growth factors are promoted to gather towards a white membrane layer, the preparation speed of the PRP is promoted, the PRP with enough concentration can be obtained through single centrifugation, the PRP with high concentration can be obtained quickly, and the preparation time and the infection risk are reduced.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention.
Claims (10)
- A PRP centrifuge sleeve, comprising: comprises an outer sleeve (1) and a spiral coil (2);the outer sleeve (1) is used for being connected with a rotating shaft of a centrifuge, and the spiral coil (2) and the blood collection tube are arranged in the outer sleeve (1);the spiral coil (2) is arranged on the inner side of the outer sleeve (1), and two connecting ends of the spiral coil (2) are used for being connected with a power supply outside the outer sleeve (1);spiral coil (2) are around the axis spiral winding of outer sleeve (1), spiral coil (2) are used for twineing in the periphery of the heparin tube of packing into in outer sleeve (1).
- 2. The PRP centrifugal sleeve of claim 1, wherein: the blood collection tube is characterized by further comprising an inner sleeve (3), wherein the inner sleeve (3) is used for inserting a blood collection tube;the inner sleeve (3) is inserted into the inner side of the outer sleeve (1), and the spiral coil (2) is wound on the periphery of the inner sleeve (3) and the periphery of the blood sampling tube inserted into the inner sleeve (3).
- 3. The PRP centrifugal sleeve of claim 2, wherein: the sleeve opening of the inner sleeve (3) is covered by the sleeve cover (4).
- 4. The PRP centrifugal sleeve of claim 3, wherein: the sleeve opening of the outer sleeve (1) is provided with external threads, the sleeve cover (4) is provided with internal threads, and the sleeve cover (4) is in threaded connection with the outer sleeve (1).
- 5. The PRP centrifugal sleeve of any one of claims 2-4, wherein: the outer sleeve (1) and the inner sleeve (3) are fixedly connected or detachably connected.
- A PRP centrifuge, characterized by: comprising a centrifuge body (5) and a PRP centrifuge sleeve according to any of claims 1-5;the centrifuge body (5) comprises a sleeve frame (6) and a pulse current generator (7); the sleeve frame (6) is fixed on a rotating shaft of the centrifuge main body (5), and the outer sleeve (1) is installed on the sleeve frame (6); the pulse current generator (7) is installed on the centrifuge body (5), and the pulse current generator (7) is electrically connected with the two connecting ends of the spiral coil (2) through electric wires.
- 7. The PRP centrifuge of claim 6, wherein: the sleeve frame (6) comprises an upper connecting arm (61) and a lower connecting arm (62) arranged below the upper connecting arm (61); the upper connecting arm (61) is connected with the part, close to the pipe orifice, of the outer sleeve (1), and the lower connecting arm (62) is connected with the part, close to the pipe tail, of the outer sleeve (1).
- 8. The PRP centrifuge of claim 7, wherein: last linking arm (61) of telescopic frame (6) are at least two, all it winds to go up linking arm (61) included angles such as the pivot of centrifuge main part (5) arrange, linking arm (62) correspond the setting about and with the quantity unanimity of last linking arm (61) down, and are every right go up linking arm (61) with linking arm (62) are connected one down outer sleeve (1).
- 9. The PRP centrifuge of claim 7 or 8, wherein: each upper connecting arm (61) is provided with an upper electric wire (81), and two ends of each upper electric wire (81) are respectively and electrically connected with one connecting end of the pulse current generator (7) and the spiral coil (2); the lower connecting arm (62) is provided with a lower electric wire (82), and two ends of the lower electric wire (82) are respectively and electrically connected with the pulse current generator (7) and the other connecting end of the spiral coil (2).
- A process for preparing PRP using the PRP centrifuge of any one of claims 6 to 9 for preparing concentrated PRP and separating platelet poor plasma fractions, comprising the steps of:s1: collecting venous blood in a blood collection tube under aseptic conditions;s2: placing the blood collection tube with the collected venous blood into a PRP centrifugal sleeve;s3: setting centrifugal force and centrifugal time;s4: and (3) introducing pulse alternating current into the spiral coil of the PRP centrifugal sleeve when the centrifugation is started, so that the magnetic field with the direction changed by the spiral coil acts on the charged growth factors in the blood collection tube, and the charged growth factors are promoted to be gathered towards the leucoderma layer to quickly obtain the PRP with high concentration.
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