CN105214343A - A kind of extraction suit of platelet rich plasma - Google Patents
A kind of extraction suit of platelet rich plasma Download PDFInfo
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- CN105214343A CN105214343A CN201510701168.XA CN201510701168A CN105214343A CN 105214343 A CN105214343 A CN 105214343A CN 201510701168 A CN201510701168 A CN 201510701168A CN 105214343 A CN105214343 A CN 105214343A
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Abstract
<b> the invention discloses a kind of extraction suit of platelet rich plasma, comprise the first syringe, second syringe, push rod and protective cap, the leading section of the first syringe offers blood sampling hole, protective cap is removably set on the leading section of the first syringe, second syringe can be plugged in the rearward end of the first syringe along the longitudinal direction slidably, the leading section of the second syringe is arranged with first piston, first piston seals setting mutually with the inwall of the first syringe, the rearward end that push rod is plugged in the second syringe can be slided along the inner wall sealing of the second syringe, extract suit and also comprise the flexible pipe running through first piston axial vane surface, for being communicated with the inner chamber of the first syringe and the second syringe.Adopt the suit that extracts of the present invention from blood sampling, centrifugal to obtaining plateletrich whole process all completing under an airtight environment, effectively stop the possibility by bacterium or virus contamination, and this leaching process is convenient and easy, the rich platelet purity obtained is high, and the equipment cost adopted is low.</b>
Description
Technical field
the present invention relates to technical field of medical instruments, particularly a kind of extraction suit of platelet rich plasma.
Background technology
at present, the recovery of blood and separation process are generally all based on blood taking needle, syringe, centrifuge tube, a series of medical appliance such as entry needle, use program complexity, the medical disposable material related to is also many, and in operating process, effectively can not avoid the pollution again of the external environments such as air, inevitable impact is caused on the degree of purity of blood, even in the process stored, also there is certain haemolysis, blood coagulation, the phenomenons such as the free hemoglobin inflammatory mediator containing variable concentrations in blood and cell-stimulating product, also may be mixed with the impurity particles such as microtissue chip and micro-aggressiveness such as lipochondrion simultaneously, various bad reaction may be caused, thus certain applications are limited.Equipment component unavoidably remains red blood cell component, does not more have the function removing the impurity such as leucocyte, have impact on coagulation function, needs to supplement blood platelet in addition, and operation inconvenience, equipment is costly.
at present, have also appeared following several extraction element, as patent (application number: 201320801217.3) disclose a kind of high concentration platelet rich plasma extraction element, this device is made up of liquid flowing tube, pipette, adapter sleeve, make blood in centrifugal separation processes, avoid contaminated possibility by this device, but inevitably there occurs with outside air when venous blood is moved in pipette from liquid flowing tube and contact, there is contaminated possibility.Same, patent of invention (application number is 201310539497.X) discloses a kind of blood rich platelet separating and extracting process and device, the extraction element that this patent adopts, although ensure that centrifugal process is in an airtight space, but, when adopting this extraction suit to carry out separation and Extraction, still need first to use injector for medical purpose venous blood samples, venous blood in syringe is injected into after in this separating extraction device and carries out centrifugally operated again, in the process of transfer venous blood, still inevitably come in contact with outside air, there is contaminated possibility equally.It can thus be appreciated that existing extraction element, from blood sampling, centrifugal to extraction, still can not thoroughly completely cut off in whole process and the contact of external environment, inevitably pollution is to a certain degree caused to blood.
Summary of the invention
the object of this invention is to provide a kind of extraction suit of platelet rich plasma, this extraction suit cost is low, easy to use, overall process no bacteria pollution, efficiently solve medical separating blood device step used in the prior art complicated, can not effectively Control pollution and the high problem of cost.
for achieving the above object, the technical solution used in the present invention is: a kind of extraction suit of platelet rich plasma, described extraction suit comprises the first syringe, second syringe, push rod and protective cap, the leading section of described first syringe offers blood sampling hole, described protective cap is removably set on the leading section of described first syringe, described second syringe can be plugged in the rearward end of described first syringe along the longitudinal direction slidably, the leading section of described second syringe is arranged with first piston, described first piston seals setting mutually with the inwall of described first syringe, the rearward end that described push rod is plugged in described second syringe can be slided along the inner wall sealing of described second syringe, described extraction suit also comprises the flexible pipe running through described first piston axial vane surface, described flexible pipe is for being communicated with the inner chamber of described first syringe and described second syringe.
preferably, described extraction suit also comprises the 3rd syringe, the rearward end that described push rod is plugged in described 3rd syringe can be slided along the inner wall sealing of described 3rd syringe, described 3rd syringe can be plugged in the rearward end of described second syringe along the longitudinal direction slidably, the leading section of described 3rd syringe is arranged with the second piston sealing setting with the inwall of described second syringe mutually, described second piston has the inner chamber of hollow, described extraction suit also comprises the filter for removing white blood cell be located in described second piston cavity, the axial forward end face of described second piston offers the piston hole communicated with himself inner chamber.
further preferably, the filter membrane for leukocyte depletion is provided with in described filter for removing white blood cell.
still more preferably, described filter membrane is laminated by the hydrophilic melt-blown fine resin fabric nonwoven cloth of multilayer tool after modification, and wherein, the fiber string diameter of described non-woven fabrics is 6.25-25 μm, and non-woven fabrics aperture is 15-60 μm.
further preferably, described filter for removing white blood cell is threaded mutually with described 3rd syringe.
preferably, described extraction suit also comprises the threeway syringe needle be threaded mutually with described blood sampling hole, one end of described threeway syringe needle be provided with for described blood sampling hole be threaded mutually pullover, all the other two ends are respectively equipped with blood taking needle and entry needle.
preferably, described extraction suit also comprises the locking mechanism locked for both be located between described first syringe and described second syringe, described locking mechanism is used for preventing described extraction to be sleeved in centrifugal process, and described second syringe and described first syringe are separated.
further preferably, described locking mechanism comprises the stop part of the band draw-in groove be located in described first back end of syringe portion, the lug boss be located in described second syringe outer peripheral sidewall, described lug boss matches with the size of described draw-in groove, when described boss is on the length bearing of trend of described draw-in groove, described locking mechanism unlocks, and described second syringe can along wall slip in described first syringe; When described lug boss departs from the length bearing of trend of described draw-in groove, described locking mechanism locking, described second syringe is plugged in the rearward end of described first syringe still.
preferably, the leading section of described push rod is also arranged with the 3rd piston sealing setting with described second syringe inwall mutually, described push rod is slided by the inner wall sealing of described 3rd piston along described second syringe.
preferably, the outer peripheral sidewall in described blood sampling hole offers external screw thread, the madial wall of described protective cap offers the internal thread with described blood sampling hole phase threaded engagement.
due to the utilization of technique scheme, the present invention compared with prior art has following advantages: the extraction suit of platelet rich plasma of the present invention, its structure is simple, by piston push pull with realize by the erythrocyte in blood, blood plasma is separated, the part stayed is platelet rich plasma, from blood sampling, centrifugal to extracting, whole operating process is all complete in airtight extraction suit, effectively completely cut off and contacted with external environments such as air, avoid bacterium and the pollution of virus to blood, reduce the impact on blood platelet result of use, enhance hematoblastic service efficiency, and alleviate the financial burden of patient, and by increase syringe and filter for removing white blood cell, also can eliminate leucocyte by obtaining, blood platelet after the impurity such as some microtissue chips and micro-polymers, thus be conducive to improving hematoblastic storage quality, avoid the complication that leucocyte brings.
Accompanying drawing explanation
the structural representation that the extraction that accompanying drawing 1 is the platelet rich plasma described in embodiment 1 is set with;
the sectional view that the extraction that accompanying drawing 2 is the platelet rich plasma described in embodiment 1 is set with;
the structural representation that the extraction that accompanying drawing 3 is the platelet rich plasma described in embodiment 2 is set with;
the sectional view that the extraction that accompanying drawing 4 is the platelet rich plasma described in embodiment 2 is set with;
accompanying drawing 5 is the structural representation of threeway syringe needle in the extraction suit of platelet rich plasma of the present invention;
accompanying drawing 6 is the structural representation of stop part on the first syringe in the extraction suit of platelet rich plasma of the present invention;
wherein: 11, the first syringe; 110, blood sampling hole; 111, stop part; 112, draw-in groove; 12, the second syringe; 121, lug boss; 13, the 3rd syringe; 14, push rod; 15, protective cap; 16, threeway syringe needle; 160, pullover; 161, blood taking needle; 162, entry needle; 163, blood sampling valve; 164, introduction valve;
21, first piston; 22, the second piston; 23, the 3rd piston; 24, filter for removing white blood cell; 25, flexible pipe.
Detailed description of the invention
below in conjunction with drawings and the specific embodiments, the present invention will be further described in detail.
embodiment 1
shown in Fig. 1, Fig. 2, a kind of extraction suit of platelet rich plasma, this extraction suit comprises the first syringe 11, second syringe 12, push rod 14 and protective cap 15.
the leading section of this first syringe 11 offers blood sampling hole 110, protective cap 15 is removably set on the leading section of the first syringe 11.In order to prevent in centrifugal process; there is protective cap 15 and depart from the first syringe 11; cause blood sampling hole 110 in atmosphere exposed; cause the phenomenon of the pollution of venous blood; in this example; the outer peripheral sidewall in this blood sampling hole 110 offers external screw thread, the inwall of protective cap 15 offers the internal thread with this blood sampling hole 110 phase threaded engagement.
this second syringe 12 can be plugged in the rearward end of the first syringe 11 along the longitudinal direction slidably, the leading section of the second syringe 12 is arranged with first piston 21, this first piston 21 seals setting mutually with the inwall of the first syringe 11, here, in order to improve the fastness of connection, the leading section of this second syringe 12 offers the externally threaded interface of band, this first piston 21 is threaded mutually with the interface on the leading section of the second syringe 12.The rearward end that this push rod 14 is plugged in the second syringe 12 can be slided along the inner wall sealing of the second syringe 12.Here, the leading section of this push rod 14 is also arranged with the 3rd piston 23,3rd piston 23 seals setting mutually with the inwall of the second syringe 12, by the 3rd piston 23, this push rod 14 slides along the inner wall sealing of the second syringe 12, certainly, in order to the fixing connected, the 3rd piston 23 can adopt the mode be threaded to be connected on the leading section of this push rod 14.
this extraction suit also comprises the flexible pipe 25 running through first piston 21 axial vane surface, and this flexible pipe 25 is for being communicated with the inner chamber of the first syringe 11 and the second syringe 12.
this extraction suit also comprises the threeway syringe needle 16 be threaded mutually with blood sampling hole 110, one end of this threeway syringe needle 16 be provided with for blood sampling hole 110 be threaded mutually pullover 160, all the other two ends are respectively equipped with blood taking needle 161 and entry needle 162, this threeway syringe needle 16 also comprise be located at blood taking needle 161 one end for control the blood sampling valve 163 of blood sampling situation, entry needle 162 one end for controlling the introduction valve 164(of ejection situation as shown in Figure 5).
adopt the concrete steps of said extracted suit extraction platelet rich plasma as follows:
1) blood collection
threeway syringe needle 16 and extraction suit adopt oxirane to carry out sterilizing by 1.1, are then connected on the blood sampling hole 110 of extraction suit by threeway syringe needle 16 by pullover 160;
1.2 select puncture vessel to take a blood sample, blood vessel should select the high-visible vein not easily slided of ancon, as median basilic vein, cephalic vein, basilic vein etc., during blood sampling, first blood sampling site is carried out disinfection, then confirm that the blood sampling valve 163 on threeway syringe needle 16 is opened, introduction valve 164 cuts out, pull second syringe 12, carry out extraction blood to extracting in suit by blood taking needle 161, the barrel of the first syringe 11 is carved with volume markings, by observing scale to determine the blood flow volume extracted;
after 1.3 blood samplings, confirm that the blood sampling valve 163 on threeway syringe needle 16 cuts out, introduction valve 164 is opened, the blood in the anti-coagulants being extracted aequum by entry needle 162 is set with extraction mixes;
1.4 back out threeway syringe needle 16, are set in by protective cap 15 on blood sampling hole 110, complete the collection of blood sample;
2) centrifugation
extraction suit is vertically placed in centrifuge by 2.1, and wherein, blood sampling hole 110 is downward, and the physiological saline installing corresponding weight with balance pipe additional balances, heavily centrifugation;
2.2 is centrifugal to blood layering, takes out and extract suit, take off protective cap 15, extraction suit is promoted the second syringe 12 vertically downward gently, drives first piston 21 to move down, the red blood cell being positioned at lower floor in the first syringe 11 is extruded, abandon it, leave middle blood platelet and the blood plasma on upper strata;
2.3 put protective cap 15 again, extraction suit is placed in centrifuge that to carry out second time centrifugal, after centrifugal, take out extraction element, take off protective cap 15, extraction suit is promoted the second syringe 12 vertically downward gently, drives first piston 21 to move down, remaining red blood cell in the first syringe 11 is extruded;
3) extraction is detached
after removing red blood cell, vertically pull push rod 14 gently, the 3rd piston 23 is driven to move up, by the flexible pipe 25 running through first piston 21 axial vane surface, the blood platelet being positioned at the first syringe 11 lower floor is sucked the second syringe 12, obtain the platelet rich plasma needed for us, and the blood plasma stayed in the first syringe 11, pushed away by promoting the second syringe 12 gently.
4) install and use
when platelet rich plasma uses, screwed out by the second syringe 12 from the first syringe 11, removal first piston 21, the interface of the second syringe 12 leading section installs entry needle 162 or spraying nozzle can use wound.
here, in order to prevent in centrifugal process, the first syringe 11 and the second syringe 12 are separated.In the present embodiment, this extraction suit also comprises the locking mechanism locked for both be located between the first syringe 11 and the second syringe 12.This locking mechanism comprises the stop part 111 of the band draw-in groove 112 be located in the first syringe 11 rearward end, the lug boss 121 be located in the second syringe 12 outer peripheral sidewall, as shown in Figure 6, this lug boss 121 matches with the size of draw-in groove 112, when on the length bearing of trend that this lug boss 121 is positioned at draw-in groove 112, this locking mechanism unlocks, and the second syringe 12 can along wall slip in the first syringe 11; When lug boss 121 departs from the length bearing of trend of draw-in groove 112, locking mechanism locks, second syringe 12 is plugged in the rearward end of the first syringe 11 still, draw-in groove 112 is aimed at for the ease of lug boss 121, be marked with ON index line at the lateral wall subscript of this first syringe 11, the length bearing of trend of this index line is consistent with the length bearing of trend of this draw-in groove 112.Concrete, when centrifugally operated need be carried out, then rotate the second syringe 12, make the lug boss 121 in the second syringe 12 outer peripheral sidewall depart from ON index line on the first syringe 11 lateral wall, locking mechanism is locked; When after centrifugal end, when need carry out draw operation to the second syringe 12, then rotate the second syringe 12, make the lug boss 121 in the second syringe 12 outer peripheral sidewall aim at ON index line.
embodiment 2
the difference part of the present embodiment and embodiment 1 is, adds the 3rd syringe 13 and filter for removing white blood cell 24 in the present embodiment, thus can further by Impurity removals such as the leucocyte in blood platelet, some microtissue chips and micro-polymers.
in the present embodiment, shown in Fig. 3, Fig. 4, this extraction suit also comprises the 3rd syringe 13, and the rearward end that push rod 14 is plugged in the 3rd syringe 13 can be slided along the inner wall sealing of the 3rd syringe 13, and the 3rd syringe 13 can be plugged in the rearward end of the second syringe 12 along the longitudinal direction slidably.Here, same, the leading section of push rod 14 is arranged with the 3rd piston the 23, three piston 23 and seals setting mutually with the inwall of the 3rd syringe 13.The leading section of the 3rd syringe 13 is arranged with the second piston 22 sealing setting with the inwall of the second syringe 12 mutually, this the second piston 22 has the inner chamber of hollow, this extraction suit also comprises the filter for removing white blood cell 24 be located in this second piston 22 inner chamber, and the axial forward end face of this second piston 22 offers the piston hole communicated with himself inner chamber.In order to ensure the fastness connected, this filter for removing white blood cell 24, second piston 22 is threaded mutually with the 3rd syringe 13 respectively.Here, the leading section of the 3rd syringe 13 is also provided with the externally threaded interface of band, this filter for removing white blood cell 24 is threaded mutually with this interface.
in the present embodiment, the filter membrane for leukocyte depletion is provided with in this filter for removing white blood cell 24, this filter membrane is laminated by the hydrophilic melt-blown fine resin fabric nonwoven cloth of multilayer tool after modification, wherein, the fiber string diameter of the non-woven fabrics of every layer is 6.25-25 μm, and non-woven fabrics aperture is 15-60 μm.Here, PBT or PET resin fiber can be selected to prepare above-mentioned non-woven fabrics.Adopt the filter membrane that above-mentioned material is made, its filtering accuracy is high.Blood platelet after this filter membrane, is the platelet rich plasma eliminating the impurity such as leucocyte, some microtissue chips and micro-polymers.
the extraction of the present embodiment is sleeved on extracts in plateletrich process, on the basis of embodiment 1, after eliminating red blood cell, pull the 3rd syringe 13, the blood platelet in the first syringe 11 is made to be pumped in the second syringe 12 by flexible pipe 25, then promote the second syringe 12 unnecessary blood plasma is extruded, then pull push rod 14, blood platelet in second syringe 12 is pumped in the 3rd syringe 13 after the second piston 22, filter for removing white blood cell 24, now, the blood platelet in the 3rd syringe 13 is the platelet rich plasma eliminating the impurity such as leucocyte.During use, the 3rd syringe 13 is screwed out in the second syringe 12, screws out the second piston 22 and filter for removing white blood cell 24, the leading section interface of the 3rd syringe 13 installs entry needle 162 or spraying nozzle can use wound.
to sum up, the extraction suit of platelet rich plasma of the present invention, from blood sampling, all complete in closed system in the centrifugal whole process to obtaining platelet rich plasma, efficiently avoid in prior art, the pollution problem that may run into when extracting platelet rich plasma, can by the plasma removing on the red blood cell of lower floor after layering in blood and upper strata by pull syringe, obtain platelet rich plasma, and by increasing filter for removing white blood cell 24, can by other some Impurity removals such as leucocyte in platelet rich plasma, obtain eliminating leukocytic platelet rich plasma, efficiently avoid the complication that in use leucocyte brings.
above-described embodiment is only for illustrating technical conceive of the present invention and feature; its object is to person skilled in the art can be understood content of the present invention and be implemented; can not limit the scope of the invention with this; all equivalences done according to Spirit Essence of the present invention change or modify, and all should be encompassed in protection scope of the present invention.
Claims (10)
1. the extraction suit of a platelet rich plasma, it is characterized in that: described extraction suit comprises the first syringe, second syringe, push rod and protective cap, the leading section of described first syringe offers blood sampling hole, described protective cap is removably set on the leading section of described first syringe, described second syringe can be plugged in the rearward end of described first syringe along the longitudinal direction slidably, the leading section of described second syringe is arranged with first piston, described first piston seals setting mutually with the inwall of described first syringe, the rearward end that described push rod is plugged in described second syringe can be slided along the inner wall sealing of described second syringe, described extraction suit also comprises the flexible pipe running through described first piston axial vane surface, described flexible pipe is for being communicated with the inner chamber of described first syringe and described second syringe.
2. the extraction suit of platelet rich plasma according to claim 1, it is characterized in that: described extraction suit also comprises the 3rd syringe, the rearward end that described push rod is plugged in described 3rd syringe can be slided along the inner wall sealing of described 3rd syringe, described 3rd syringe can be plugged in the rearward end of described second syringe along the longitudinal direction slidably, the leading section of described 3rd syringe is arranged with the second piston sealing setting with the inwall of described second syringe mutually, described second piston has the inner chamber of hollow, described extraction suit also comprises the filter for removing white blood cell be located in described second piston cavity, the axial forward end face of described second piston offers the piston hole communicated with himself inner chamber.
3. the extraction suit of platelet rich plasma according to claim 2, is characterized in that: be provided with the filter membrane for leukocyte depletion in described filter for removing white blood cell.
4. the extraction suit of platelet rich plasma according to claim 3, it is characterized in that: described filter membrane is laminated by the hydrophilic melt-blown fine resin fabric nonwoven cloth of multilayer tool after modification, wherein, the fiber string diameter of described non-woven fabrics is 6.25-25 μm, and non-woven fabrics aperture is 15-60 μm.
5. the extraction suit of platelet rich plasma according to claim 2, is characterized in that: described filter for removing white blood cell is threaded mutually with described 3rd syringe.
6. the extraction suit of platelet rich plasma according to claim 1, it is characterized in that: described extraction suit also comprises the threeway syringe needle be threaded mutually with described blood sampling hole, one end of described threeway syringe needle be provided with for described blood sampling hole be threaded mutually pullover, all the other two ends are respectively equipped with blood taking needle and entry needle.
7. according to the extraction suit of the arbitrary described platelet rich plasma of claim 1 to 6, it is characterized in that: described extraction suit also comprises the locking mechanism locked for both be located between described first syringe and described second syringe, described locking mechanism is used for preventing described extraction to be sleeved in centrifugal process, and described second syringe and described first syringe are separated.
8. the extraction suit of platelet rich plasma according to claim 7, it is characterized in that: described locking mechanism comprises the stop part of the band draw-in groove be located in described first back end of syringe portion, the lug boss be located in described second syringe outer peripheral sidewall, described lug boss matches with the size of described draw-in groove, when described boss is on the length bearing of trend of described draw-in groove, described locking mechanism unlocks, and described second syringe can along wall slip in described first syringe; When described lug boss departs from the length bearing of trend of described draw-in groove, described locking mechanism locking, described second syringe is plugged in the rearward end of described first syringe still.
9. the extraction suit of platelet rich plasma according to claim 1, it is characterized in that: the leading section of described push rod is also arranged with the 3rd piston sealing setting with described second syringe inwall mutually, described push rod is slided by the inner wall sealing of described 3rd piston along described second syringe.
10. the extraction suit of platelet rich plasma according to claim 1, is characterized in that: the outer peripheral sidewall in described blood sampling hole offers external screw thread, the madial wall of described protective cap offers the internal thread with described blood sampling hole phase threaded engagement.
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