CN109332007A - A kind of plasma separating unit and plasma separation method - Google Patents

A kind of plasma separating unit and plasma separation method Download PDF

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Publication number
CN109332007A
CN109332007A CN201811202512.0A CN201811202512A CN109332007A CN 109332007 A CN109332007 A CN 109332007A CN 201811202512 A CN201811202512 A CN 201811202512A CN 109332007 A CN109332007 A CN 109332007A
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CN
China
Prior art keywords
straight tube
piston
bar
plasma
shaped bar
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Granted
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CN201811202512.0A
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Chinese (zh)
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CN109332007B (en
Inventor
程晔
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Jiangsu Cancer Hospital
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Jiangsu Cancer Hospital
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Priority to CN201811202512.0A priority Critical patent/CN109332007B/en
Publication of CN109332007A publication Critical patent/CN109332007A/en
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Publication of CN109332007B publication Critical patent/CN109332007B/en
Expired - Fee Related legal-status Critical Current
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B5/00Other centrifuges
    • B04B5/04Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers
    • B04B5/0407Radial chamber apparatus for separating predominantly liquid mixtures, e.g. butyrometers for liquids contained in receptacles
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B7/00Elements of centrifuges
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B04CENTRIFUGAL APPARATUS OR MACHINES FOR CARRYING-OUT PHYSICAL OR CHEMICAL PROCESSES
    • B04BCENTRIFUGES
    • B04B9/00Drives specially designed for centrifuges; Arrangement or disposition of transmission gearing; Suspending or balancing rotary bowls
    • B04B9/08Arrangement or disposition of transmission gearing ; Couplings; Brakes

Abstract

The present invention relates to a kind of plasma separating unit and plasma separation method, device specifically includes fixing seat, the first straight tube, the second straight tube, observation piston, first piston, telescopic rod, the first U-shaped bar, the second U-shaped bar, nut set and screw rod;Second straight tube is placed in fixing seat;Observation piston is longitudinally slidably arranged in the second straight tube, and observation piston is connected with second piston bar made of transparent material;Second piston bar and the axle center perforation for observing piston are provided with capillary channel;The end of first straight tube is provided with connector and the first straight tube is docked via the connector with the end that second piston bar is located at outside the second straight tube;Telescopic rod is longitudinally disposed at the side of fixing seat, the first U-shaped bar horizontal connection in telescopic rod end and be fastened in the horizontal U-lag of the first U-shaped bar;Screw rod is rotationally longitudinally disposed at the other side of fixing seat, and screw rod is drivingly connected via nut set, the second U-shaped bar and the second straight tube;It is precisely controllable that the invention enables plasma separations.

Description

A kind of plasma separating unit and plasma separation method
Technical field
The present invention relates to a kind of plasma separating unit and plasma separation methods.
Background technique
Existing blood plasma separation test generally use centrifugal device by anticoagulation centrifugation separation after, manual operation Pasteur Suction pipe carries out layering absorption to the blood after layering, and the separation process is for the more demanding of operator, specifically, needing It wants operator's eye and the cooperation of hand height that could separate successfully, middle layer or even red blood cell is otherwise easy to cause to be mixed into blood plasma.
Summary of the invention
Present invention aims at solving the above problems, a kind of plasma separating unit and plasma separation method, tool are provided Body is realized by the following technical scheme:
A kind of plasma separating unit, including it is fixing seat, the first straight tube, the second straight tube, observation piston, first piston, flexible Bar, the first U-shaped bar, the second U-shaped bar, nut set and screw rod;Second straight tube is placed in the fixing seat, and second The end of straight tube, which is provided with, takes orgnaizer of illegal blood donation;The observation piston is longitudinally slidably arranged in the second straight tube, observation piston connection There is second piston bar made of transparent material, described second piston bar one end is pierced by the second straight tube;Second piston bar and observation The axle center perforation of piston is provided with capillary channel;The end of first straight tube be provided with connector and the first straight tube via The connector is docked with the end that second piston bar is located at outside the second straight tube, so that the capillary channel and described the Connection inside one straight tube;The first piston is longitudinally slidably arranged in the first straight tube, and the first piston is connected with It is pierced by the First piston bar of first straight tube;The telescopic rod is longitudinally disposed at the side of the fixing seat, the first U For the horizontal connection of shape bar in the end of the telescopic rod, the First piston bar is fastened in the horizontal U-lag of the first U-shaped bar It is interior;The screw rod is rotationally longitudinally disposed at the other side of the fixing seat, and the nut external threading is connected to the spiral shell Bar, in the nut set, the outer wall of first straight tube is provided with first annular slot, described for the second U-shaped bar horizontal connection Second U-shaped bar is fastened in the first annular slot;First straight tube, the internal diameter of the second straight tube are equal, in second straight tube Have the whole blood that plasma layer, middle layer and red blood cell layer are formed by centrifugation layering, when rotating the screw rod, the nut While set drives first straight tube and observation piston downlink to push plasma layer, formed inside the first straight tube corresponding negative It presses, is advanced into the first straight tube on blood plasma, until middle layer enters in the capillary channel in observation piston and second piston bar So that the color in second piston bar axle center changes, stop operating screw rod, then blood plasma fully enters the first straight tube, middle layer And a small amount of red blood cell is located in capillary channel.
The plasma separating unit, further design is, the side of the fixing seat is horizontally disposed sliding rail, Sliding rack is slidably provided on the sliding rail, the screw rod is rotationally disposed longitudinally on sliding rack;Sliding rack direction When fixing seat is mobile, the first annular slot of second U-shaped bar clamping the first straight tube outer wall, sliding rack is far from fixing seat movement When, the U-lag of the second U-shaped bar is detached from the first annular slot.
The plasma separating unit, further design is, driving motor is provided on the sliding rack and is subtracted Fast transmission mechanism, the driving motor are connect via reduction gearing mechanism with the screw drive.
The plasma separating unit, further design is, nut set side is connected with guide sleeve, and Sliding rack is vertically arranged with guide rod in the side of the screw rod, and the guide sleeve is set on the guide rod.
The plasma separating unit, further design is, the reduction gearing mechanism is one group of transmission gear.
The plasma separating unit, further design is that the outer wall of the second piston bar is connected with the second ring Shape slot, is symmetrically arranged with luminous tube and light-sensitive element in second annular groove, in the light-sensitive element and second piston bar Capillary channel it is opposite;The light-sensitive element is connect with a controller signals, the control unit of the controller and the driving motor Signal connection;When driving motor starts, luminous tube, the light that the light-sensitive element detection comes from the transmission of capillary channel direction are opened Line, in optical signal mutation, controller, which issues instruction, drives driving motor to stop operation.
The plasma separating unit, further design is that the luminous tube, light-sensitive element are respectively arranged at one In the shell of a sector, the internal diameter of two fan-shaped shells is equal with the outer diameter of the second piston bar, and two fan-shaped It is interference fitted between shell and the second annular groove;The side of light-sensitive element towards second piston bar offers and capillary channel phase Pair loophole.
The plasma separating unit, further design is that the outer wall of second straight tube is arranged with straight barrel type Locking set, the upper inside wall for locking set are provided with the circular groove of the second annular groove lower wall described in clamping, and its underpart inner wall is provided with pressure There is the rubber layer of higher stiction together in the second straight tube outer wall, the locking set is made of the docking of two and half straight tubes.
The plasma separating unit, further design is, the connector of first straight tube passes through screw thread and institute State the end connection of second piston bar.
A kind of plasma separation method, the process employs a kind of plasma separating unit, the plasma separating unit includes solid Reservation, the first straight tube, the second straight tube, observation piston, first piston, telescopic rod, the first U-shaped bar, the second U-shaped bar, nut set with And screw rod;Second straight tube is placed in the fixing seat, and the end of the second straight tube, which is provided with, takes orgnaizer of illegal blood donation;The observation piston Longitudinally slidably it is arranged in the second straight tube, observation piston is connected with second piston bar made of transparent material, and described second Piston rod one end is pierced by the second straight tube;Second piston bar and the axle center perforation for observing piston are provided with capillary channel;Described The end of one straight tube is provided with connector and the first straight tube is located at outside the second straight tube via the connector and second piston bar End docking so that the capillary channel is connected to first straight tube inside;The first piston can be slided longitudinally It is arranged in the first straight tube dynamicly, the first piston is connected with the First piston bar for being pierced by first straight tube;It is described flexible Bar is longitudinally disposed at the side of the fixing seat, and the first U-shaped bar horizontal connection is in the end of the telescopic rod, and described One piston rod is fastened in the horizontal U-lag of the first U-shaped bar;The screw rod is rotationally longitudinally disposed at the fixation The other side of seat, the nut external threading are connected to the screw rod, and the 2nd U shape bar horizontal connection is in the nut set, institute The outer wall for stating the first straight tube is provided with first annular slot, and the second U-shaped bar is fastened in the first annular slot;Described first Straight tube, the internal diameter of the second straight tube are equal;
It is anticoagulant to specifically comprise the following steps: that 1, the twitch second piston bar and observation piston are taken using the second straight tube Blood;2, make whole blood after the first straight tube, first piston, observation piston and the second straight tube being integrally put into centrifugal device rotation Centrifugation layering forms plasma layer, middle layer and red blood cell layer;3, the second straight tube is put into fixing seat, and makes first to live Stopper rod enters in the U-lag of the first U-shaped bar, adjusts telescopic rod height so that First piston bar clamping and the first U-shaped bar to It is unable to downlink, and rotates screw rod and makes the second U-shaped bar contour with the first annular slot and be held in the second U-shaped bar In first annular slot;4, the screw rod is rotated, the nut set drives first straight tube and observation piston downlink to push blood While pulp layer, corresponding negative pressure is formed inside the first straight tube, is advanced into the first straight tube on blood plasma, until middle layer enters In capillary channel in observation piston and second piston bar the color in second piston bar axle center is changed, stops turning Dynamic screw rod;5, telescopic rod height is adjusted, so that the U-lag of First piston bar and the first U-shaped bar disengages, then by the first straight tube It is removed from the end of second piston bar, is blood plasma in the first straight tube, middle layer or even a small amount of red blood cell are located at capillary channel Interior, remaining red blood cell is located in the second straight tube.
The beneficial effects of the present invention are:
By the way that transparent second piston bar is arranged between the first straight tube and the second straight tube and utilizes screw rod and nut set The first straight tube and observation piston is adjusted in the screw mechanism of formation, so that in the first straight tube in plasma separation Negative pressure it is precisely controllable, and by the fluid column color of the capillary channel in observation second piston bar rather than plasma layer and intermediate Liquid level position between layer makes separation critical point more obvious, so that will not be contaminated with middle layer in the blood plasma of the first straight tube Or even red blood cell;During first straight tube and observation piston downlink while make the pressure increase in the second straight tube, the Corresponding negative pressure is formed in one straight tube, so that liquid level held stationary, stratification state will not be destroyed;Utilize luminous tube, photosensitive member Part can replace human eye and be monitored to the color of the fluid column in capillary channel, further improve operation precision.
Detailed description of the invention
Fig. 1 is the overall structure diagram of the embodiment of the present invention.
Fig. 2 is the second U-shaped bar, nut set and guide sleeve structure schematic diagram.
Fig. 3 is luminous tube, light-sensitive element structural relation schematic diagram.
Fig. 4 is lock sleeve schematic diagram of internal structure.
Fig. 5 is lock sleeve external structure schematic diagram.
Fig. 6 is latch segment side structure schematic diagram on the outside of lock sleeve edge.
Fig. 7 is the U-shaped card structure schematic diagram connecting with latch segment.
Fig. 8 is partial structural diagram at A in Fig. 1.
Fig. 9 is status diagram when accommodating anticoagulation in the second straight tube.
Figure 10 is that the second straight tube is located at embodiment status diagram when in fixing seat after blood is layered.
Figure 11 is that blood plasma enters embodiment status diagram after the first straight tube.
Specific embodiment
Below in conjunction with Figure of description and embodiment, invention is further explained:
Plasma separating unit as shown in Figure 1 includes fixing seat 10, the first straight tube 1, the second straight tube 2, observation piston 3, the One piston 4, telescopic rod 5, the first U-shaped bar 6, the 2nd U shape bar 7, nut set 8 and screw rod 9;Second straight tube 2 is placed in fixing seat 10 It is interior, and the end of the second straight tube is provided with and takes orgnaizer of illegal blood donation;Observation piston is longitudinally slidably arranged in the second straight tube 2, sees It examines piston 3 and is connected with second piston bar 31 made of transparent material, 31 one end of second piston bar is pierced by the second straight tube 2;Second is living Stopper rod 31 and the axle center perforation for observing piston are provided with capillary channel 32;The end of first straight tube be provided with connector and First straight tube is docked via the connector with the end that second piston bar is located at outside the second straight tube, so that capillary channel 32 are connected to the first straight tube inside;First piston 4 is longitudinally slidably arranged in the first straight tube 1, and first piston 4 is connected with It is pierced by the First piston bar 41 of the first straight tube;Telescopic rod is longitudinally disposed at the side of fixing seat, 6 horizontal connection of the first U-shaped bar in The end of telescopic rod, First piston bar are fastened in the horizontal U-lag of the first U-shaped bar 6;Screw rod 9 is rotationally longitudinally disposed In the other side of fixing seat, nut set 8 is threadedly connected to screw rod, and 7 horizontal connection of the 2nd U shape bar is in nut set, the first straight tube 1 Outer wall be provided with first annular slot 11, the second U-shaped bar is fastened in first annular slot 11;First straight tube 1, the second straight tube 2 it is interior Diameter is equal, and the whole blood for forming plasma layer, middle layer and red blood cell layer by centrifugation layering is had in the second straight tube, rotates spiral shell When bar, while nut set drives the first straight tube and observation piston downlink to push plasma layer, formed inside the first straight tube corresponding Negative pressure, be advanced into the first straight tube on blood plasma, until to enter the capillary in observation piston and second piston bar logical for middle layer The color in second piston bar axle center is made to change in road, stop operating screw rod, then blood plasma fully enters the first straight tube, in Interbed and a small amount of red blood cell are located in capillary channel.
The side of fixing seat 10 is horizontally disposed sliding rail 101, and sliding rack 102, spiral shell are slidably provided on sliding rail 101 Bar is rotationally disposed longitudinally on sliding rack;When sliding rack is mobile towards fixing seat, outside 7 the first straight tube of clamping of the second U-shaped bar The first annular slot of wall, when sliding rack is mobile far from fixing seat, the U-lag of the second U-shaped bar 7 is detached from first annular slot 11.
Screw rod can be rotated using handle manually, which is not present high-frequency vibration, in workbench and the On the basis of two U-shaped bars eliminate most of vibration using vibration-absorptive material, screw rod also can use motor and be driven, it is specific and It says, is provided with driving motor 103 and reduction gearing mechanism 104 on sliding rack 102, driving motor is via reduction gearing mechanism It is connect with screw drive.Reduction gearing mechanism is one group of transmission gear.
As shown in connection with fig. 2,8 sides of nut set are connected with guide sleeve 81, and sliding rack is longitudinally disposed in the side of screw rod There is guide rod 82, guide sleeve is worn on the guide bar.
As shown in connection with fig. 3, the outer wall of second piston bar 31 is connected with the second annular groove 33, symmetrically sets in the second annular groove It is equipped with luminous tube 34 and light-sensitive element 35, light-sensitive element is opposite with the capillary channel in second piston bar;Light-sensitive element and one The control unit signal of controller signals connection, the controller and driving motor connects;When driving motor starts, luminous tube is opened, The light that light-sensitive element detection comes from the transmission of capillary channel direction, since the translucency of plasma layer is compared with middle layer and red blood cell Layer is more preferable, enters capillary in middle layer and red blood cell layer and is mutated with optical signal, controller issues instruction and drives driving motor It stops operation.
Luminous tube 34, light-sensitive element 35 are respectively arranged in a fan-shaped shell, the internal diameter of two fan-shaped shells with The outer diameter of second piston bar is equal, and is interference fitted between two fan-shaped shells and the second annular groove;Light-sensitive element direction The side of second piston bar offers the loophole opposite with capillary channel.
In conjunction with Fig. 4-Fig. 7, shown, the outer wall of the second straight tube 2 is arranged with the locking set 21 of straight barrel type, locking set 21 it is upper Portion's inner wall is provided with the circular groove 211 of clamping the second annular groove lower wall, and its underpart inner wall, which is provided with, is pressed on the second straight tube outer wall tool There is the rubber layer 212 of higher stiction, locking set is made of the docking of two and half straight tubes.On the outside of the edge of two and half straight tubes It is provided with latch segment 213, latch segment makes the docking of two and half straight tubes become locking set after fixing via U-shaped card 214.Locking set For when entering centrifugal device separation after the second straight tube takes anticoagulation by second piston bar, second piston or even the first straight tube Keep relatively fixed with the stack shell of the second straight tube, at this point, circular groove 211 is engaged the second annular groove, and rubber layer 212 squeezes second The outer wall of straight tube forms stiction;And after blood plasma enters the first straight tube, locking set can be once again secured in second In straight tube, so that second piston bar is fixed relative to the second straight tube, consequently facilitating the first straight tube is removed.
As shown in figure 8, the connector of the first straight tube is connected by the end of screw thread and second piston bar.
A kind of plasma separation method specifically comprises the following steps: 1, as shown in figure 9, twitching second piston bar and observation Piston takes anticoagulation using the second straight tube;2, the first straight tube, first piston, observation piston and the second straight tube are integrally put into Whole blood centrifugation layering is made to form plasma layer, middle layer and red blood cell layer after centrifugal device rotation;3, as shown in Figure 1, by Two straight tubes are put into fixing seat, and First piston bar is entered in the U-lag of the first U-shaped bar, adjust telescopic rod height, make First piston bar clamping and the first U-shaped bar to be unable to downlink, and rotate screw rod make the second U-shaped bar with it is first annular Slot is contour and the 2nd U shape bar is held in first annular slot;4, screw rod as shown in Figure 10, is rotated, nut set drives the While one straight tube and observation piston downlink push plasma layer, corresponding negative pressure is formed inside the first straight tube, is advanced on blood plasma Enter in the first straight tube, until making second piston in the capillary channel that middle layer enters in observation piston and second piston bar The color in bar axle center changes, and stop operating screw rod;5, telescopic rod height as shown in figure 11, is adjusted, so that First piston bar It disengages with the U-lag of the first U-shaped bar, then removes the first straight tube from the end of second piston bar, be blood in the first straight tube Slurry, middle layer or even a small amount of red blood cell are located in capillary channel, remaining red blood cell is located in the second straight tube.

Claims (10)

1. a kind of plasma separating unit, it is characterised in that live including fixing seat, the first straight tube, the second straight tube, observation piston, first Plug, telescopic rod, the first U-shaped bar, the second U-shaped bar, nut set and screw rod;Second straight tube is placed in the fixing seat, and And second the end of straight tube be provided with and take orgnaizer of illegal blood donation;The observation piston is longitudinally slidably arranged in the second straight tube, and observation is lived Plug is connected with second piston bar made of transparent material, and described second piston bar one end is pierced by the second straight tube;Second piston bar with And the axle center perforation of observation piston is provided with capillary channel;The end of first straight tube is provided with connector and the first straight tube Docked via the connector with the end that second piston bar is located at outside the second straight tube so that the capillary channel with it is described Connection inside first straight tube;
The first piston is longitudinally slidably arranged in the first straight tube, and the first piston, which is connected with, is pierced by described first directly The First piston bar of cylinder;The telescopic rod is longitudinally disposed at the side of the fixing seat, and the first U-shaped bar horizontal connection is in institute The end of telescopic rod is stated, the First piston bar is fastened in the horizontal U-lag of the first U-shaped bar;The screw rod can be rotated Ground is longitudinally disposed at the other side of the fixing seat, and the nut external threading is connected to the screw rod, and the second U-shaped bar is horizontal It is connected to the nut set, the outer wall of first straight tube is provided with first annular slot, and the second U-shaped bar is fastened in described One annular groove;First straight tube, the internal diameter of the second straight tube are equal, have in second straight tube and form blood by centrifugation layering The whole blood of pulp layer, middle layer and red blood cell layer, when rotating the screw rod, the nut set drives first straight tube and sight While examining piston downlink pushing plasma layer, corresponding negative pressure is formed inside the first straight tube, is advanced into the first straight tube on blood plasma, Until sending out the color in second piston bar axle center in the capillary channel that middle layer enters in observation piston and second piston bar Changing, stop operating screw rod, then blood plasma fully enters the first straight tube, and middle layer and a small amount of red blood cell are located at capillary channel It is interior.
2. plasma separating unit according to claim 1, which is characterized in that the side of the fixing seat is horizontally disposed cunning Rail is slidably provided with sliding rack on the sliding rail, and the screw rod is rotationally disposed longitudinally on sliding rack;Sliding rack court When mobile to fixing seat, the first annular slot of second U-shaped bar clamping the first straight tube outer wall, sliding rack is far from fixing seat movement When, the U-lag of the second U-shaped bar is detached from the first annular slot.
3. plasma separating unit according to claim 2, which is characterized in that be provided on the sliding rack driving motor with And reduction gearing mechanism, the driving motor are connect via reduction gearing mechanism with the screw drive.
4. plasma separating unit according to claim 3, which is characterized in that nut set side is connected with guide sleeve, And sliding rack is vertically arranged with guide rod in the side of the screw rod, and the guide sleeve is set on the guide rod.
5. plasma separating unit according to claim 3, which is characterized in that the reduction gearing mechanism is one group of driving cog Wheel.
6. plasma separating unit according to claim 3, which is characterized in that the outer wall of the second piston bar is connected with Second ring slot is symmetrically arranged with luminous tube and light-sensitive element, the light-sensitive element and second piston in second annular groove Capillary channel in bar is opposite;The light-sensitive element is connect with a controller signals, the control of the controller and the driving motor Signal connection in portion's processed;When driving motor starts, luminous tube is opened, what the light-sensitive element detection came from the transmission of capillary channel direction Light, in optical signal mutation, controller, which issues instruction, drives driving motor to stop operation.
7. plasma separating unit according to claim 6, which is characterized in that the luminous tube, light-sensitive element are respectively set In in a fan-shaped shell, the internal diameter of two fan-shaped shells is equal with the outer diameter of the second piston bar, and two fans It is interference fitted between the shell of shape and the second annular groove;The side of light-sensitive element towards second piston bar offers and capillary channel Opposite loophole.
8. plasma separating unit according to claim 6, which is characterized in that the outer wall of second straight tube is arranged with straight tube The locking set of type, the upper inside wall for locking set are provided with the circular groove of the second annular groove lower wall described in clamping, the setting of its underpart inner wall Have and be pressed on the rubber layer that the second straight tube outer wall has higher stiction, the locking set is by two and half straight tube docking groups At.
9. plasma separating unit according to claim 1, which is characterized in that the connector of first straight tube passes through screw thread It is connect with the end of the second piston bar.
10. a kind of plasma separation method, which is characterized in that the process employs a kind of plasma separating unit, the blood plasma separation Device includes fixing seat, the first straight tube, the second straight tube, observation piston, first piston, telescopic rod, the first U-shaped bar, the second U-shaped Bar, nut set and screw rod;Second straight tube is placed in the fixing seat, and the end of the second straight tube, which is provided with, takes orgnaizer of illegal blood donation;Institute It states observation piston to be longitudinally slidably arranged in the second straight tube, observation piston is connected with second piston made of transparent material Bar, described second piston bar one end are pierced by the second straight tube;Second piston bar and the axle center perforation for observing piston are provided with capillary Channel;The end of first straight tube is provided with connector and the first straight tube via the connector and second piston bar is located at the End docking outside two straight tubes, so that being connected to inside the capillary channel and first straight tube;The first piston Longitudinally slidably it is arranged in the first straight tube, the first piston is connected with the First piston bar for being pierced by first straight tube; The telescopic rod is longitudinally disposed at the side of the fixing seat, the first U-shaped bar horizontal connection in the end of the telescopic rod, The First piston bar is fastened in the horizontal U-lag of the first U-shaped bar;The screw rod is rotationally longitudinally disposed at described The other side of fixing seat, the nut external threading are connected to the screw rod, and the second U-shaped bar horizontal connection is in the nut Set, the outer wall of first straight tube are provided with first annular slot, and the second U-shaped bar is fastened in the first annular slot;It is described First straight tube, the internal diameter of the second straight tube are equal;
Specifically comprise the following steps: that 1, the twitch second piston bar and observation piston take anticoagulation using the second straight tube;2, First straight tube, first piston, observation piston and the second straight tube are integrally put into after centrifugal device rotation and make whole blood centrifugation point Layer forms plasma layer, middle layer and red blood cell layer;3, the second straight tube is put into fixing seat, and First piston bar is entered In the U-lag of first U-shaped bar, telescopic rod height is adjusted, so that First piston bar clamping is unable to downlink with the first U-shaped bar, And it rotates screw rod to make the second U-shaped bar contour with the first annular slot and the second U-shaped bar is held in first annular slot It is interior;4, the screw rod is rotated, while the nut set drives first straight tube and observation piston downlink to push plasma layer, Form corresponding negative pressure inside first straight tube, be advanced into the first straight tube on blood plasma, until middle layer enter observation piston and The color in second piston bar axle center is made to change in capillary channel in second piston bar, stop operating screw rod;5, it adjusts Telescopic rod height, so that the U-lag of First piston bar and the first U-shaped bar disengages, then by the first straight tube from second piston bar End is removed, and is blood plasma in the first straight tube, and middle layer or even a small amount of red blood cell are located in capillary channel, remaining red blood cell is located at In second straight tube.
CN201811202512.0A 2018-10-16 2018-10-16 Plasma separation device and plasma separation method Expired - Fee Related CN109332007B (en)

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