CN103405818A - Device and method for preparing platelet-rich plasma - Google Patents
Device and method for preparing platelet-rich plasma Download PDFInfo
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- CN103405818A CN103405818A CN2013103854748A CN201310385474A CN103405818A CN 103405818 A CN103405818 A CN 103405818A CN 2013103854748 A CN2013103854748 A CN 2013103854748A CN 201310385474 A CN201310385474 A CN 201310385474A CN 103405818 A CN103405818 A CN 103405818A
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Abstract
The invention relates to a device and method for preparing platelet-rich plasma. The device comprises a piston, a concave outer chamber tube, a convex outer chamber tube, a piston sleeve, an inner chamber tube, a push-pull rod, a needle and an outer cover. The piston sleeve is arranged in the convex outer chamber tube, the piston sleeve moves along the inner wall of the convex outer chamber tube, the inner chamber tube is arranged in the piston sleeve, the piston sleeve is in threaded connection with the inner chamber tube, the push-pull rod is arranged in the inner chamber tube, the push-pull rod moves along the inner wall of the inner chamber tube, a central hole is formed in the center of the piston sleeve, one end of the central hole leads to the inner cavity of the convex outer chamber tube, the other end of the central hole leads to the inner cavity of the inner chamber tube, a boss protruding outwards is arranged at the front end of the convex outer chamber tube, a needle hole is formed in the center of the boss, the needle is inserted in the needle hole, or the boss is sleeved with the outer cover. Or the boss is provided with the concave outer chamber tube, the piston is arranged in the concave outer chamber tube, the piston moves along the inner wall of the concave outer chamber tube, a blood inlet is formed in the center of the front end of the concave outer chamber tube, one end of the blood inlet is communicated with the concave outer chamber tube, and the other end of the blood inlet is communicated with the needle hole.
Description
Technical field
The present invention relates to a kind of device from the mixed layer of isolating plasma layer and platelet and blood plasma blood and preparation method thereof.
Background technology
Injured or postoperative each recovery process is the challenge to physical ability regeneration.Platelet plays an important role in regenerative process.Platelet can promote the healing of solidifying of blood and wound, simultaneously important effect is also arranged in tissue regeneration, because they can produce courier's material, and so-called somatomedin namely.These somatomedin absorb stem cell and reserve cell, and induce differentiation.By the increment new organization, just formed.
In stem cell and reserve cell, somatomedin is serving as " Magnet ".If the ability of " Magnet " is stronger, will absorb more stem cell and reserve cell so, thus the reduction healing time.
As can be seen here, most important from blood, isolating platelet, but complicated from blood, isolating hematoblastic device at present, and will in blood, add activator (as solidifyingization enzyme) and activate or the hematoblastic release of catalysis.
Summary of the invention
The object of the invention is to overcome the defect of prior art, and provide the simple syringe of a kind of use and the centrifuge just can be from device of isolating plasma layer and platelet and blood plasma mixed layer blood and preparation method thereof.
In order to complete purpose of the present invention, the present invention by the following technical solutions:
A kind of device that is rich in platelet blood plasma for preparing of the present invention, it comprises: piston, recessed mistress's pipe, protruding mistress's pipe, piston bush, the inner room pipe, push-pull bar, syringe needle and enclosing cover, wherein: in protruding mistress manages, piston bush is housed, piston bush moves along protruding mistress's inside pipe wall, the inner room pipe is housed in piston bush, piston bush with the inner room pipe by being threaded, in the inner room pipe, push-pull bar is housed, push-pull bar moves along the inner room inside pipe wall, at the piston bush center, there is an end of this centre bore of centre bore to lead to the inner chamber of protruding mistress's pipe, the other end leads to the inner chamber of inner room pipe, the front end of protruding mistress's pipe is a boss of outwards giving prominence to, central authorities at this boss have a pin hole, in pin hole, be inserted with syringe needle, perhaps cover has enclosing cover on boss, perhaps at boss, recessed mistress's pipe is housed, in recessed mistress manages, piston is housed, this piston moves along recessed mistress's inside pipe wall, the front end centre of recessed mistress's pipe has blood receiving hole, one end of blood receiving hole communicates with recessed mistress's pipe, the other end communicates with pin hole,
The device of platelet blood plasma is rich in preparation of the present invention, wherein: the place ahead of described protruding mistress's pipe is outwards outstanding taper shape, it is cambered inwards taper shape that recessed mistress manages the place ahead, recessed mistress's pipe intercouples with protruding mistress's pipe, and the shape of the front end of piston bush is identical with the inner chamber front end shape of protruding mistress's pipe;
The device of platelet blood plasma is rich in preparation of the present invention, wherein: described piston bush is comprised of sleeve pipe and nose cone, and nose cone, at the front end of sleeve pipe, has centre bore in cone headed central authorities, the inner room pipe is contained in sleeve pipe, and the front end of inner room pipe is connected with nose cone by screw thread;
The device of platelet blood plasma is rich in preparation of the present invention, and wherein: described sleeve pipe and nose cone are connected as a single entity;
The device of platelet blood plasma is rich in preparation of the present invention, wherein: described enclosing cover by thread set on boss;
The device of platelet blood plasma is rich in preparation of the present invention, wherein: described recessed mistress's pipe by thread set on boss;
The device of platelet blood plasma is rich in preparation of the present invention, and wherein: the volume of described recessed mistress's pipe is 50ml to 200ml.
With device of the present invention, prepare the method that is rich in platelet blood plasma, comprise the following steps:
(A) piston bush is contained in protruding mistress manages, the inner room pipe is contained in piston bush, and push-pull bar is contained in the inner room pipe, and syringe needle is inserted on the pin hole of protruding mistress's pipe, pulls out piston bush and inner room pipe, by blood sampling, is flowed in the inner chamber of protruding mistress's pipe;
(B) syringe needle is extracted from protruding mistress's pipe, enclosing cover on the boss upper cover of protruding mistress's pipe, then it is shaken up, enclosing cover straight down, protruding mistress's pipe, piston bush, inner room pipe and push-pull bar are put into to the centrifugally operated for the first time that centrifuge carried out 5 minutes to 10 minutes together, and the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins;
(C) by said apparatus from centrifuge, vertically taking out, blood divides mixed layer and the plasma layer of protometrocyte layer, platelet and hemocyte from bottom to up in protruding mistress's tube cavity, enclosing cover is pulled out, the recessed mistress's pipe of access and piston on boss, piston is moved down along recessed mistress's pipe, by after the recessed mistress's pipe of undermost hemocyte layer suction in protruding mistress's tube cavity, recessed mistress's pipe to be extracted from protruding mistress's pipe, the hemocyte layer is stored in the inner chamber of recessed mistress's pipe;
(D) enclosing cover is covered again on the boss of protruding mistress's pipe, then it is shaken up, enclosing cover straight down, protruding mistress's pipe, piston bush, inner room pipe and push-pull bar are put into to the centrifugally operated for the second time that centrifuge carried out 5 minutes to 10 minutes together, and the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins;
(E) by said apparatus from centrifuge, vertically taking out, blood divides mixed layer and the plasma layer of thromboblast and hemocyte from bottom to up in the inner chamber of protruding mistress's pipe, push-pull bar is moved up along the inner room pipe, by after the plasma layer suction inner room pipe of the superiors in protruding mistress's tube cavity, then the inner room pipe is extracted from protruding mistress's pipe; Plasma layer is stored in the inner room tube cavity; The mixed layer of platelet and hemocyte is stored in recessed mistress's tube cavity, has realized platelet and the mixed layer of hemocyte and separating of plasma layer.
Method of the present invention, wherein: the rotating speed of described centrifuge is 1500 rev/mins.
The platelet blood plasma that is rich in from concrete conditions in the establishment of a specific crime in this invention can straightforward manipulation, acquisition inside hospital operating room or office treatment.In addition, only need to use double-chamber syringe (or the first injection emitter directly directly is connected with second syringe), and, without using a large amount of syringes, so just exempted necessity of extra injection device and storage container, reduce the danger of polluting.The main advantage that is rich in platelet blood plasma method that is obtained from concrete conditions in the establishment of a specific crime is exactly that double syringe can shift to less syringe from larger syringe, and without using several syringes.In addition, in the blood of extraction, do not contain any activator (as solidifyingization enzyme) and activate or the hematoblastic release reaction of catalysis, namely before centrifugal, among without add any can be from activator or the material of platelet h substance.The risk of polluting also reduces greatly, and the complexity of process also weakens.
The accompanying drawing explanation
Fig. 1 is under the blood sampling state, and the device of platelet blood plasma is rich in preparation of the present invention;
Fig. 2 be apocarpy for the first time and centrifugal for the second time under, the device of platelet blood plasma is rich in preparation of the present invention;
Fig. 3 is after centrifugal for the first time, and the original state of the device of platelet blood plasma is rich in the preparation of the present invention of extracting hemocyte;
Fig. 4 is after centrifugal for the first time, and the end-state of the device of platelet blood plasma is rich in the preparation of the present invention of extracting hemocyte;
Fig. 5 is after centrifugal for the second time, and the device of platelet blood plasma is rich in the preparation of the present invention of extracting blood plasma.
In Fig. 1 to Fig. 5, label 1 is piston; Label 2 is recessed mistress's pipe; Label 3 is for being coated with enclosing cover; Label 4 is protruding mistress's pipe; Label 5 is piston bush; Label 6 is the inner room pipe; Label 7 is push-pull bar; Label 8 is syringe needle; Hole centered by label 9; Label 10 is sleeve pipe; Label 11 is nose cone; Label 12 is pin hole; Label 13 is boss; Label 14 is blood receiving hole.
The specific embodiment
As shown in Figures 1 to 5, a kind of device that is rich in platelet blood plasma for preparing of the present invention comprises: piston 1, recessed mistress manage 2, protruding mistress manages 4, piston bush 5, inner room pipe 6, push-pull bar 7, syringe needle 8 and enclosing cover 9, and it is 50ml to 200ml that recessed mistress manages 2 volume.
Protruding mistress manages in 4 is equipped with piston bush 5, piston bush 5 is managed 4 inwalls along protruding mistress and is moved, inner room pipe 6 is housed in piston bush 5, piston bush 5 with inner room pipe 6 by being threaded, push-pull bar 7 is housed in inner room pipe 6, push-pull bar 7 moves along inner room pipe 6 inwalls, one centre bore 9 is arranged at the center of piston bush 5, one end of this centre bore 9 leads to protruding mistress manages 4 inner chamber, the other end leads to the inner chamber of inner room pipe 6, it is an outside outstanding boss 13 that protruding mistress manages 4 front end, central authorities at this boss 13 have a pin hole 12, as shown in Figure 1, in pin hole 12, be inserted with syringe needle 8, perhaps as shown in Figure 2, enclosing cover 3 by thread set on boss 13, perhaps as shown in Figure 3 and Figure 4, recessed mistress manage 2 by thread set on boss 13, recessed mistress, manage in 2 piston 1 is housed, this piston 1 is managed 2 inwalls along recessed mistress and is moved, recessed mistress manages 2 front end centre and has blood receiving hole 14, one end of blood receiving hole 14 is managed 2 with recessed mistress and is communicated, the other end communicates with pin hole 12.It is outwards outstanding taper shape that protruding mistress manages the place ahead of 4, and it is cambered inwards taper shapes that recessed mistress manages 2 the place aheads, and recessed mistress manages 2 and with protruding mistress, manages 4 and intercouple, and it is identical that the shape of the front end of piston bush 5 and protruding mistress manage 4 inner chamber front end shape.Piston bush 5 is comprised of sleeve pipe 10 and nose cone 11, and nose cone 11 is at the front end of sleeve pipe 10, has centre bore 9 in the central authorities of nose cone 11, and inner room pipe 6 is contained in sleeve pipe 10, and the front end of inner room pipe 6 is connected with nose cone 11 by screw thread.Sleeve pipe 10 and nose cone 11 are connected as a single entity.
By apparatus of the present invention, prepare the method that is rich in platelet blood plasma, comprise the following steps:
(A) as shown in Figure 1, piston bush 5 is contained in to protruding mistress and manages in 4, inner room pipe 6 is contained in piston bush 5, push-pull bar 7 is contained in inner room pipe 6, syringe needle 8 is inserted in protruding mistress and manages on 4 pin hole 12, pulls out piston bush 5 and inner room pipe 6, by blood sampling, is flowed into protruding mistress and manages in 4 inner chamber;
(B) as shown in Figure 2, syringe needle 8 is managed to 4 from protruding mistress to be extracted, protruding mistress, manage enclosing cover 3 on 4 boss 13 upper covers, then it is shaken up, enclosing cover 3 straight down, by protruding mistress manage 4, piston bush 5, inner room pipe 6 and push-pull bar 7 put into the centrifugally operated for the first time that centrifuge carried out 5 minutes to 10 minutes together, the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins, is preferably 1500 rev/mins;
(C) as shown in Figure 3 and Figure 4, by said apparatus from centrifuge, vertically taking out, blood manages protruding mistress mixed layer and the plasma layer that 4 interior intracavity divide protometrocyte layer, platelet and hemocyte from bottom to up, enclosing cover 3 is pulled out, the recessed mistress of access manages 2 and piston 1 on boss 13, piston 1 is managed to 2 along recessed mistress to be moved down, protruding mistress is managed in 4 inner chambers after the recessed mistress of undermost hemocyte layer suction manages 2, recessed mistress is managed to 2 and from protruding mistress, manage 4 and extract, the hemocyte layer is stored in recessed mistress and manages in 2 inner chamber;
(D) as shown in Figure 2, enclosing cover 3 is covered again on protruding mistress manages 4 boss 13, then it is shaken up, enclosing cover 3 straight down, by protruding mistress manage 4, piston bush 5, inner room pipe 6 and push-pull bar 7 put into the centrifugally operated for the second time that centrifuge carried out 5 minutes to 10 minutes together, the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins, is preferably 1500 rev/mins;
(E) as shown in Figure 5, by said apparatus from centrifuge, vertically taking out, blood divides mixed layer and the plasma layer of thromboblast and hemocyte from bottom to up in protruding mistress manages 4 inner chamber, push-pull bar 7 is moved up along inner room pipe 6, after protruding mistress being managed to the plasma layer suction inner room pipe 6 of the superiors in 4 inner chambers, then inner room pipe 6 is managed to 4 from protruding mistress and extract; Plasma layer is stored in inner room pipe 6 inner chambers; The mixed layer of platelet and hemocyte is stored in recessed mistress and manages in 2 inner chambers, has realized platelet and the mixed layer of hemocyte and separating of plasma layer.
Above description is explanation of the invention, is not that limited range of the present invention is referring to claim, in the situation that without prejudice to spirit of the present invention, the present invention can do the modification of appropriate format to the restriction of invention.
Claims (9)
1. one kind prepares the device that is rich in platelet blood plasma, it comprises: piston (1), recessed mistress's pipe (2), protruding mistress's pipe (4), piston bush (5), inner room pipe (6), push-pull bar (7), syringe needle (8) and enclosing cover (9), it is characterized in that: in protruding mistress's pipe (4), piston bush (5) is housed, piston bush (5) is managed (4) inwall along protruding mistress and is moved, inner room pipe (6) is housed in piston bush (5), piston bush (5) with inner room pipe (6) by being threaded, push-pull bar (7) is housed in inner room pipe (6), push-pull bar (7) moves along inner room pipe (6) inwall, at the center of piston bush (5), a centre bore (9) is arranged, one end of this centre bore (9) leads to the inner chamber of protruding mistress's pipe (4), the other end leads to the inner chamber of inner room pipe (6), the front end of protruding mistress's pipe (4) is a boss (13) of outwards giving prominence to, central authorities at this boss (13) have a pin hole (12), in pin hole (12), be inserted with syringe needle (8), perhaps at the upper cover of boss (13), enclosing cover (3) is arranged, recessed mistress's pipe (2) perhaps is housed on boss (13), in recessed mistress's pipe (2), piston (1) is housed, this piston (1) is managed (2) inwall along recessed mistress and is moved, the front end centre of recessed mistress's pipe (2) has blood receiving hole (14), one end of blood receiving hole (14) is managed (2) with recessed mistress and is communicated, the other end communicates with pin hole (12).
2. the device of platelet blood plasma is rich in preparation as claimed in claim 1, it is characterized in that: the place ahead of described protruding mistress's pipe (4) is outwards outstanding taper shape, recessed mistress's pipe (2) the place ahead is cambered inwards taper shape, recessed mistress's pipe (2) intercouples with protruding mistress's pipe (4), and the shape of the front end of piston bush (5) is identical with the inner chamber front end shape that protruding mistress manages (4).
3. the device of platelet blood plasma is rich in preparation as claimed in claim 2, it is characterized in that: described piston bush (5) is comprised of sleeve pipe (10) and nose cone (11), nose cone (11) is at the front end of sleeve pipe (10), central authorities at nose cone (11) have centre bore (9), inner room pipe (6) is contained in sleeve pipe (10), and the front end of inner room pipe (6) is connected with nose cone (11) by screw thread.
4. the device of platelet blood plasma is rich in preparation as claimed in claim 3, it is characterized in that: described sleeve pipe (10) and nose cone (11) are connected as a single entity.
5. the device of platelet blood plasma is rich in preparation as claimed in claim 1, it is characterized in that: described enclosing cover (3) by thread set on boss (13).
6. the device of platelet blood plasma is rich in preparation as described as claim 1 or 5, it is characterized in that: described recessed mistress pipe (2) by thread set on boss (13).
7. the device of platelet blood plasma is rich in preparation as claimed in claim 6, it is characterized in that: the volume of described recessed mistress's pipe (2) is 50ml to 200ml.
8. with the arbitrary device of claim 1 to 7, prepare the method that is rich in platelet blood plasma, comprise the following steps:
(A) piston bush (5) is contained in protruding mistress's pipe (4), inner room pipe (6) is contained in piston bush (5), push-pull bar (7) is contained in inner room pipe (6), syringe needle (8) is inserted on the pin hole (12) of protruding mistress's pipe (4), pull out piston bush (5) and inner room pipe (6), by blood sampling, flowed in the inner chamber of protruding mistress's pipe (4);
(B) syringe needle (8) being managed to (4) from protruding mistress extracts, enclosing cover (3) on boss (13) upper cover of protruding mistress's pipe (4), then it is shaken up, enclosing cover (3) straight down, protruding mistress's pipe (4), piston bush (5), inner room pipe (6) and push-pull bar (7) are put into to the centrifugally operated for the first time that centrifuge carried out 5 minutes to 10 minutes together, and the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins;
(C) by said apparatus from centrifuge, vertically taking out, blood intracavity in protruding mistress manages (4) divides mixed layer and the plasma layer of protometrocyte layer, platelet and hemocyte from bottom to up, enclosing cover (3) is pulled out, at the recessed mistress's pipe of the upper access of boss (13) (2) and piston (1), piston (1) is managed to (2) along recessed mistress to be moved down, by after the recessed mistress's pipe of undermost hemocyte layer suction (2) in protruding mistress's pipe (4) inner chamber, recessed mistress's pipe (2) is managed to (4) from protruding mistress and extract, the hemocyte layer is stored in the inner chamber of recessed mistress's pipe (2);
(D) enclosing cover (3) is covered again on the boss (13) of protruding mistress's pipe (4), then it is shaken up, enclosing cover (3) straight down, protruding mistress's pipe (4), piston bush (5), inner room pipe (6) and push-pull bar (7) are put into to the centrifugally operated for the second time that centrifuge carried out 5 minutes to 10 minutes together, and the rotating speed of centrifuge is 1000 rev/mins to 2000 rev/mins;
(E) by said apparatus from centrifuge, vertically taking out, blood divides mixed layer and the plasma layer of thromboblast and hemocyte from bottom to up in protruding mistress manages the inner chamber of (4), push-pull bar (7) is moved up along inner room pipe (6), by after the plasma layer suction inner room pipe (6) of the superiors in protruding mistress's pipe (4) inner chamber, then inner room pipe (6) is managed to (4) from protruding mistress and extract; Plasma layer is stored in inner room pipe (6) inner chamber; The mixed layer of platelet and hemocyte is stored in recessed mistress's pipe (2) inner chamber, has realized platelet and the mixed layer of hemocyte and separating of plasma layer.
9. method as claimed in claim 8, it is characterized in that: the rotating speed of described centrifuge is 1500 rev/mins.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385474.8A CN103405818B (en) | 2013-08-28 | 2013-08-28 | Prepare the devices and methods therefor of Platelet-rich plasm |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310385474.8A CN103405818B (en) | 2013-08-28 | 2013-08-28 | Prepare the devices and methods therefor of Platelet-rich plasm |
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| CN103405818A true CN103405818A (en) | 2013-11-27 |
| CN103405818B CN103405818B (en) | 2016-07-06 |
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| CN201310385474.8A Active CN103405818B (en) | 2013-08-28 | 2013-08-28 | Prepare the devices and methods therefor of Platelet-rich plasm |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198229A (en) * | 2014-09-03 | 2014-12-10 | 中国科学院植物研究所 | Gas sampling injector |
| CN105596014A (en) * | 2016-01-22 | 2016-05-25 | 黄鹭强 | Double-cannula centrifugal injector |
| CN108904902A (en) * | 2018-09-14 | 2018-11-30 | 威海凯尔医疗科技有限公司 | Double syringe autologous plasma collection device and the method for collecting blood plasma |
| CN108940613A (en) * | 2018-07-17 | 2018-12-07 | 深圳市北科融汇生命科技有限公司 | Blood sample separates centrifuge and its separation method |
| CN109332007A (en) * | 2018-10-16 | 2019-02-15 | 江苏省肿瘤医院 | A kind of plasma separating unit and plasma separation method |
| CN111974475A (en) * | 2020-08-20 | 2020-11-24 | 绵阳晶凯圣生物科技有限公司 | Blood component separator |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201469752U (en) * | 2009-08-31 | 2010-05-19 | 金哲 | A device for extracting blood, separating and injecting serum, |
| CN102078644A (en) * | 2011-03-08 | 2011-06-01 | 吉林大学 | Simple, convenient and efficient device and method for extracting platelet-rich plasma |
| CN203458633U (en) * | 2013-08-28 | 2014-03-05 | 朱悦 | Device for preparing platelet rich plasma |
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2013
- 2013-08-28 CN CN201310385474.8A patent/CN103405818B/en active Active
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198229A (en) * | 2014-09-03 | 2014-12-10 | 中国科学院植物研究所 | Gas sampling injector |
| CN105596014A (en) * | 2016-01-22 | 2016-05-25 | 黄鹭强 | Double-cannula centrifugal injector |
| CN105596014B (en) * | 2016-01-22 | 2018-05-25 | 黄鹭强 | A kind of double-jacket tube centrifugation injection device |
| CN108940613A (en) * | 2018-07-17 | 2018-12-07 | 深圳市北科融汇生命科技有限公司 | Blood sample separates centrifuge and its separation method |
| CN108904902A (en) * | 2018-09-14 | 2018-11-30 | 威海凯尔医疗科技有限公司 | Double syringe autologous plasma collection device and the method for collecting blood plasma |
| CN109332007A (en) * | 2018-10-16 | 2019-02-15 | 江苏省肿瘤医院 | A kind of plasma separating unit and plasma separation method |
| CN109332007B (en) * | 2018-10-16 | 2020-12-29 | 江苏省肿瘤医院 | Plasma separation device and plasma separation method |
| CN111974475A (en) * | 2020-08-20 | 2020-11-24 | 绵阳晶凯圣生物科技有限公司 | Blood component separator |
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| CN103405818B (en) | 2016-07-06 |
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Effective date of registration: 20210113 Address after: 110000 Room 502, 18 Sanhao Street, Heping District, Shenyang City, Liaoning Province Patentee after: Shenyang kailitai Technology Co.,Ltd. Address before: No.42-1 Haikou street, Heping District, Shenyang, Liaoning 110000 Patentee before: Zhu Yue Patentee before: Ma Jinghong |
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