CN106582909A - Centrifuge tube and method for preparing platelet-rich plasma by using same - Google Patents
Centrifuge tube and method for preparing platelet-rich plasma by using same Download PDFInfo
- Publication number
- CN106582909A CN106582909A CN201611256706.XA CN201611256706A CN106582909A CN 106582909 A CN106582909 A CN 106582909A CN 201611256706 A CN201611256706 A CN 201611256706A CN 106582909 A CN106582909 A CN 106582909A
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- China
- Prior art keywords
- centrifuge tube
- centrifuge
- rich plasma
- platelet
- tube
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L9/00—Supporting devices; Holding devices
- B01L9/06—Test-tube stands; Test-tube holders
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Centrifugal Separators (AREA)
- External Artificial Organs (AREA)
Abstract
The invention relates to a medical apparatus design, in particular to a centrifuge tube and a method for preparing platelet-rich plasma. The invention discloses a method for achieving aseptic preparation of the platelet-rich plasma (PRP) by designing a centrifuge tube structure and adopting heat-sealing machine separation. The centrifuge tube provided by the invention is formed by a thin-wall hose with a sealed bottom, at least part of the centrifuge tube has a slender structure, the centrifuge tube is has a fixer suitable for a centrifuge, and the inner surface of the fixer matches the outer surface of the slender structure of the centrifuge tube. The top of the centrifuge tube is provided with a tube cover, the inner diameter of the tube cover matches the outer diameter of the centrifuge tube, the outer wall of the tube cover is provided with antiskid stripes along the axial direction, and the tube body of the centrifuge tube is provided with measurement scales. The invention also provides the method for preparing the platelet-rich plasma based on the centrifuge tube. The platelet-rich plasma provided by the invention has the characteristics of being simple in preparation method, pollution-free in a preparation process, high in purity of the platelet-rich plasma (PRP), easy in storage, convenient in use, and the like.
Description
Technical field
The present invention relates to a kind of Design for Medical Device, more particularly to a kind of centrifuge tube and its prepare the side of platelet rich plasma
Method.
Background technology
Platelet rich plasma (PRP) contains substantial amounts of growth factor, such as platelet derived growth factor (PDGF), conversion life
The long factor-β (TGF-β), insulin-like growth factor (IGF), EGF (EGF), VEGF
(VEGF) etc., these growth factors are mainly secreted by after platelet activation, can promote cell and matrix regeneration, promotion organization
Repair, rapid hemostasis, pain relieving, accelerating wound healing dramatically mitigate the formation of scar after the operation, therefore are widely used in
Various surgical operations, openheart surgery and plastic operation, are also widely used in medical cosmetology aspect.But, existing rich platelet
Blood plasma (PRP) is prepared and is primarily present following defect:(1) platelet rich plasma (PRP) concentration is low, the rich platelet blood separated
Slurry (PRP) is pale red, and the inside is mixed with red blood cell;(2) release agent is remained, separation agent is used when blood platelet is extracted, there is residual
Stay;(3) extraction process and preparation method are complicated, are to obtain higher concentration platelet rich plasma (PRP) and need to repeatedly implement separation to carry
Take, Centrifugal tube structure is complicated, easily cause platelet rich plasma (PRP) pollution.
The content of the invention
The present invention proposes to be constructed by design centrifuge tube, is separated using heat-sealing machine, realizes platelet rich plasma (PRP)
Prepare.The present invention has preparation method simple, and preparation process is pollution-free, and platelet rich plasma (PRP) purity is high, easily storage, makes
Use convenient advantage.
The present invention is realized using following technical scheme:
Described centrifuge tube is made up of the thin walled hose of sealed bottom, and the centrifuge tube is at least part of for elongated knot
Structure, described centrifuge tube has the fixator suitable for centrifuge, and fixator inner surface is mutually fitted with centrifuge tube slim-lined construction outer surface
Should.
Described centrifuge tube is in elongated tubular product, and platelet rich plasma (PRP) is in section in elongated tubular after blood sample centrifugation layering
Shape is distributed.
Described centrifuge tube is in dumbbell shaped, and platelet rich plasma (PRP) is located at thin in the middle of dumbbell shaped after blood sample centrifugation layering
Long position.
Lid is arranged at described centrifuge tube top, and lid internal diameter is adapted with centrifuge tube external diameter, and lid outer wall is provided with vertically
Anti-slip veins.
Described centrifuge tube pipe shaft has metering scale, convenient metering.
Described fixator includes being fixed with the male of the convex key of socket and is fixed with the female of interface groove, the convex key of socket with
Interface groove match.
Present disclosure also including the preparation method of described platelet rich plasma (PRP), is comprised the following steps:
Step A, blood sample is inserted quantitative in the centrifuge tube for fill anticoagulant, covered and enclosed;
Step B, centrifuge tube is put into after special fixator, uses centrifuge;
Step C, after sample centrifugation layering, is separated thrombocyte plasma part heat seal in centrifuge tube using heat-sealing machine.
The invention has the beneficial effects as follows:
(1) centrifuge tube of the present invention some be slim-lined construction, improve platelet rich plasma extracting concentration.
(2) centrifuge tube of the present invention is thin walled hose, can pass through the aseptic separation of heat-sealing machine.
(2) it is of the present invention to be easy to storage, convenient use Jing after aseptic heat seal.
Description of the drawings
Fig. 1 is a kind of centrifuge tube example structure figure;
Fig. 2 is a kind of centrifuge tube example structure figure;
Fig. 3 is a kind of centrifuge tube example structure figure;
Fig. 4 is a kind of centrifugation tube fixer embodiment front view;
Fig. 5 is a kind of centrifugation tube fixer embodiment front view;
A kind of centrifugation tube fixer embodiment top views of Fig. 6;
In figure:Centrifuge tube 1;Lid 2;Centrifugation tube fixer 3;The convex key 4 of socket;Interface groove 5.
Specific embodiment
Below in conjunction with the accompanying drawings the invention will be further described.
Embodiment 1
As shown in figure 1, centrifuge tube 1 is made up of the thin walled hose of sealed bottom, centrifuge tube 1 is in elongated tubular construction, is centrifuged
Pipe 1 has the fixator 3 suitable for centrifuge, and the inner surface of fixator 3 is adapted with the slim-lined construction outer surface of centrifuge tube 1, fixed
Device 3 includes being fixed with the male of the convex key 4 of socket and is fixed with the female of interface groove 5, the convex key 4 of socket and the phase of interface groove 5
Match somebody with somebody.Lid 2 is arranged at the top of centrifuge tube 1, and the internal diameter of lid 2 is adapted with the external diameter of centrifuge tube 1, and the outer wall of lid 2 is provided with vertically anti-slip veins.
The pipe shaft of centrifuge tube 1 has metering scale.
Embodiment 2
As shown in Fig. 2 centrifuge tube 1 is made up of the thin walled hose of sealed bottom, centrifuge tube 1 is in thin dumbbell structure, is centrifuged
The dumbbell shaped bottom of pipe 1 is circular arc bottom, and centrifuge tube 1 has the fixator 3 suitable for centrifuge.The inner surface of fixator 3 and centrifuge tube 1
Slim-lined construction outer surface is adapted, and fixator 3 includes being fixed with the male of the convex key 4 of socket and is fixed with the female of interface groove 5,
The convex key 4 of socket matches with interface groove 5.Lid 2 is arranged at the top of centrifuge tube 1, and the internal diameter of lid 2 is adapted with the external diameter of centrifuge tube 1, pipe
The outer wall of lid 2 is provided with vertically anti-slip veins.The pipe shaft of centrifuge tube 1 has metering scale.
Embodiment 3
As shown in figure 3, the dumbbell shaped bottom of centrifuge tube 1 is conical bottom.
Embodiment 4
The preparation method of platelet rich plasma (PRP), comprises the following steps:
Step A, blood sample is inserted quantitative in the centrifuge tube 1 for fill anticoagulant, covered and enclosed;
Step B, centrifuge tube 1 is put into after special fixator, uses centrifuge;
Step C, after sample centrifugation layering, is separated thrombocyte plasma part heat seal in centrifuge tube using heat-sealing machine.
Certainly, the above is only presently preferred embodiments of the present invention, it is impossible to be considered as the enforcement for limiting to the present invention
Example scope.The present invention is also not limited to the example above, essential scope of the those skilled in the art in the present invention
Interior made impartial change and improvement etc., all should belong in the patent covering scope of the present invention.
Claims (7)
1. a kind of preparation facilities of centrifuge tube, it is characterised in that:Centrifuge tube (1) is made up of the thin walled hose of sealed bottom, described
Centrifuge tube (1) is at least part of for slim-lined construction, and described centrifuge tube (1) has the fixator (3) suitable for centrifuge, fixed
Device (3) inner surface is adapted with centrifuge tube slim-lined construction outer surface.
2. the preparation facilities of centrifuge tube according to claim 1, it is characterised in that:Centrifuge tube (1) is in elongated tubular product.
3. the preparation facilities of centrifuge tube according to claim 1, it is characterised in that:Centrifuge tube (1) is in dumbbell shaped.
4. the preparation facilities of centrifuge tube according to claim 1, it is characterised in that:Lid (2) is arranged at centrifuge tube (1) top,
Lid (2) internal diameter is adapted with centrifuge tube (1) external diameter, and lid (2) outer wall is provided with vertically anti-slip veins.
5. the preparation facilities of centrifuge tube according to claim 1, it is characterised in that:Centrifuge tube (1) pipe shaft has metering scale.
6. the preparation facilities of centrifuge tube according to claim 1, it is characterised in that:Fixator (3) is including being fixed with socket
The male of convex key (4) and the female for being fixed with interface groove (5), the convex key of socket (4) matches with interface groove (5).
7. a kind of method that platelet rich plasma is prepared based on centrifuge tube, it is characterised in that comprise the following steps:
Step A, blood sample is inserted quantitative in the centrifuge tube (1) for fill anticoagulant;
Step B, centrifuge tube (1) is put into after special fixator, uses centrifuge;
Step C, after sample centrifugation layering, is separated thrombocyte plasma part heat seal in centrifuge tube (1) using heat-sealing machine.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201611256706.XA CN106582909A (en) | 2016-12-30 | 2016-12-30 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
Applications Claiming Priority (1)
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CN201611256706.XA CN106582909A (en) | 2016-12-30 | 2016-12-30 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
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CN106582909A true CN106582909A (en) | 2017-04-26 |
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CN201611256706.XA Pending CN106582909A (en) | 2016-12-30 | 2016-12-30 | Centrifuge tube and method for preparing platelet-rich plasma by using same |
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Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
CN113766938A (en) * | 2019-03-06 | 2021-12-07 | PPlus护肤有限公司 | Device for storing platelet-rich plasma |
Citations (5)
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---|---|---|---|---|
KR101194044B1 (en) * | 2011-12-29 | 2012-10-24 | (주) 알메디카 | Single unit kit for separating blood and concentrating prp and method for extracting prp using thereof |
CN102844121A (en) * | 2009-12-19 | 2012-12-26 | 全玟墉 | Centrifuge tube |
CN205042500U (en) * | 2015-09-10 | 2016-02-24 | 上海逍鹏生物科技有限公司 | Cell centrifuging tube |
CN105368706A (en) * | 2015-12-11 | 2016-03-02 | 何守贵 | High-volume mononuclear cell separating bottle |
CN206424979U (en) * | 2016-12-30 | 2017-08-22 | 山东中保康医疗器具有限公司 | Centrifuge tube |
-
2016
- 2016-12-30 CN CN201611256706.XA patent/CN106582909A/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102844121A (en) * | 2009-12-19 | 2012-12-26 | 全玟墉 | Centrifuge tube |
KR101194044B1 (en) * | 2011-12-29 | 2012-10-24 | (주) 알메디카 | Single unit kit for separating blood and concentrating prp and method for extracting prp using thereof |
CN205042500U (en) * | 2015-09-10 | 2016-02-24 | 上海逍鹏生物科技有限公司 | Cell centrifuging tube |
CN105368706A (en) * | 2015-12-11 | 2016-03-02 | 何守贵 | High-volume mononuclear cell separating bottle |
CN206424979U (en) * | 2016-12-30 | 2017-08-22 | 山东中保康医疗器具有限公司 | Centrifuge tube |
Non-Patent Citations (3)
Title |
---|
李天星主编: "《现代临床医学免疫学检验技术》", 30 September 2014, 军事医学科学出版社 * |
杨安钢等: "《生物化学与分子生物学实验技术》", 28 February 2001, 高等教育出版社 * |
胡丽华主编: "《临床输血学检验实验指导 第2版》", 31 August 2015, 中国医药科技出版社 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106979881A (en) * | 2017-05-18 | 2017-07-25 | 桂林医学院 | A kind of centrifuge tube prepared for cell block |
CN113766938A (en) * | 2019-03-06 | 2021-12-07 | PPlus护肤有限公司 | Device for storing platelet-rich plasma |
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Application publication date: 20170426 |
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