CN106076448A - A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma - Google Patents

A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma Download PDF

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Publication number
CN106076448A
CN106076448A CN201610597543.5A CN201610597543A CN106076448A CN 106076448 A CN106076448 A CN 106076448A CN 201610597543 A CN201610597543 A CN 201610597543A CN 106076448 A CN106076448 A CN 106076448A
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nipple
centrifugal
piston
long handle
type medical
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曹世奇
刘宁
陈宜
周晔
钱齐荣
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Shanghai Kai Rong Medical Technology Co Ltd
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Shanghai Kai Rong Medical Technology Co Ltd
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Priority to CN201610597543.5A priority Critical patent/CN106076448A/en
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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01DSEPARATION
    • B01D17/00Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02Separation of non-miscible liquids
    • B01D17/0217Separation of non-miscible liquids by centrifugal force
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/508Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
    • B01L3/5082Test tubes per se
    • B01L3/50825Closing or opening means, corks, bungs

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Thermal Sciences (AREA)
  • External Artificial Organs (AREA)

Abstract

The invention provides a kind of piston-type medical centrifugal, including centrifugal body and the piston being located at described centrifugal tubular body;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, the other end detachably connected nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post is detachably connected with long handle;Could be included for the aseptic package bag of all parts after depositing sterilization treatment.Present invention also offers the preparation method of a kind of platelet rich plasma, it is prepared by piston-type medical centrifugal described above.Piston-type medical centrifugal provided by the present invention can improve the convenience that PRP makes, the sample contamination occurred in operation can be avoided completely, can further improve platelet collection rate, reduce the doping of the impurity such as erythrocyte, leukocyte, it is possible to collect different types of PRP according to different occupation modes.

Description

A kind of piston-type medical centrifugal and for preparing the preparation of platelet rich plasma Method
Technical field
The present invention relates to a kind of medical apparatus and instruments, particularly relate to a kind of piston-type medical centrifugal and for preparing Fu Xue little The preparation method of plate blood plasma.
Background technology
Platelet rich plasma (PRP) is isolatable from blood, containing transforming growth factor-β (transforming growth Factor, TGF), platelet-derived growth factor (platelet-derived growth factor, PDGF), para-insulin Somatomedin (insulin-like growth factor, IGF) and VEGF (vascular Endothelial growth factor, VEGF) etc. multiple somatomedin, in addition, possibly together with leukocyte and fiber egg In vain.Multiple studies have shown that, PRP is significant to soft tissue repair, it is possible to promotes regenerating bone or cartilage, slows down cartilage degeneration simultaneously. Therefore, PRP has become the hot issue in current repair of cartilage field.
It is true that for the different tissues in human body, they are the most different for the requirement of the PRP used by reparation, such as rich white Cell PRP is suitable for and most tissue, is but not suitable for using at intraarticular.Therefore, one can need to carry out according to difference The equipment that strictly prepared by PRP is this area in the urgent need to.And current, pass through ten remainders PRP of U.S. FDA certification Preparation equipment, except product prepare single, use dumb, extract the defects such as PRP mode is limited in addition to, there is also other various respectively The deficiency of sample, number of times is loaded down with trivial details, platelet collection rate is low including expensive, centrifugal.And the similar product sold on domestic market Product, there is also various limitation, if product design is that product is prepared single only in the product of wound;And owing to design is asked Topic, blood is drawn and injects all by same suction pipe, thus remaining the impurity such as too much erythrocyte, thus affecting PRP system Standby stability.
Summary of the invention
In order to solve the deficiencies in the prior art, the invention provides a kind of piston-type medical centrifugal and for preparing richness The preparation method of thrombocyte plasma, the operation of described piston-type medical centrifugal is more convenient, preparation kind is more, range More extensively, aseptic is higher.
Subject of the present invention is a kind of piston-type medical centrifugal, including centrifugal body be located at described centrifugal tubular body Piston;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, and the other end can Releasably connect nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post removably connects with long handle Connect.
Preferably, described piston-type medical centrifugal also includes the aseptic bag of all parts after depositing sterilization treatment Pack.It is further preferred that described aseptic package bag at least contains the two identical described piston-type medical centrifugals of set, from The assemblies such as heart body, piston, nipple, external post, long handle, nipple lid, conversion mouth, through strictly sterilization treatment, are placed in one. So, as members pollutes, the most do not affect normal use;By conversion mouth, can be by close for two identical centrifugal bodys Close connection, it is achieved internal liquid is pollution-free, the transfer of No leakage ground.
Further, described centrifuge tube system become 5-100mL in the range of the utensil of specifications at different levels, with master scale line. Different amounts of platelet rich plasma can be prepared according to different demands.
Further, the main part of described centrifugal body is to be arranged above cylinder and through knot that bottom is symmetrical reverse taper Structure.This structure is conducive to blood constitutent to be enriched with in bottom, and the liquid height making same volume is higher, from described centrifugal body During the invalid component of middle discharge, can be more accurate.
Further, the high elastic rubber post of described piston contacts with described centrifugal inboard wall of tube body, makes described piston in institute State centrifugal tubular body move up and down and play sealing function, so constant at piston position or when moving down, extraneous antibacterial Will not enter in the centrifuge tube body below piston;Described piston base is reverse taper, at the bottom of its reverse taper with described centrifugal body Portion tightly coincide, and so, when piston is depressed into bottom, blood constitutent farthest can be released described centrifugal body.
Further, described nipple is divided into inside and outside two-layer, and its outer layer has screw thread or embedded ring, with described nipple lid and institute The inside thread stating conversion mouth matches, and can form screw thread or embed connection;Its internal layer is standard syringe teat dimensions, its with All kinds of syringe needles, tube for transfusion connect;Wherein, the space of 0.5-1.5mm is left between described internal layer and described outer layer.So, nipple Lid is by nipple tight closure, and closes firmly, can bear the pressure in centrifugal process;Internal layer can connection standard syringe needle, tube for transfusion, Convenient platelet rich plasma preparation completed injects desired area, it is also possible to be directly connected to tube for transfusion, from extracting in the patient Blood;The space of inside and outside two interlayers allows tube for transfusion, the insertion of the first-class general purpose module of syringe needle.
Further, described external post is external connection screw post, and it is located at the center above described piston, so can be fine Ground realizes the effect removably connected;The bottom of described long handle is provided with nut, outside its internal whorl is with described external connection screw post Portion's screw thread matches, and the upper end of described long handle is the plane for push-and-pull, and the length of described long handle is more than described centrifugal body Length, so when centrifugal, long handle can be taken off, prevent unavailable component stress, affect centrifuge results, in the non-centrifugal period, By knob, long handle can be fixed on extraneous screw thread, push-and-pull piston, draw or get rid of the liquid in centrifuge tube body.
Further, described mouth of changing is into hollow structure, and its one end is nut structure, the internal whorl of described nut structure Match with the screw thread outside described nipple;Its other end is needle hub size pipeline, screw thread or syringe needle, is used for and syringe Nipple, tube for transfusion tail end airtight connection.So can realize the multifunction of the present invention, can be complete with another by described conversion mouth Exactly the same described centrifugal body connects, it is possible to connect with other syringes or similar assembly.
The another one theme of the present invention is the preparation method of a kind of platelet rich plasma, by piston-type described above Medical centrifugal is prepared.
Preferably, at least overlap any one piston-type medical centrifugal described above by two to be prepared.
Wherein in an embodiment, described preparation method uses the rich leukocyte PRP of secondary centrifuging method preparation, including following Step:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long Handle, is expelled directly out the red blood cell layer of bottom from nipple or is collected into asepsis injector by conversion mouth, in order to being fed back to In the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged, Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will Below blood intermediate layer (rich in leukocyte, platelet) in centrifugal body and plasma layer, component is injected in syringe, is made The standby rich leukocyte PRP completed.
In one embodiment, described preparation method uses secondary centrifuging method to prepare lean leukocyte PRP, including following step Rapid:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long Handle, is expelled directly out the red blood cell layer of bottom and intermediate layer (rich in leukocyte) from nipple or is collected into nothing by conversion mouth In bacterium syringe, in order to be fed back in the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged, Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will Below blood intermediate layer (rich in platelet) in centrifugal body and plasma layer, component is injected in syringe, obtains having prepared Lean leukocyte PRP.
In one embodiment, centrifuging of described preparation method prepares lean leukocyte PRP, comprises the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long Handle, is expelled directly out the red blood cell layer of bottom and intermediate layer (rich in leukocyte) from nipple or is collected into nothing by conversion mouth In bacterium syringe, in order to be fed back in the patient;
Step 3: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will Below plasma layer in centrifugal body, component is injected in syringe, obtains the lean leukocyte PRP prepared.
It should be explicitly made clear at this point and above-mentioned the various of technical solution of the present invention are limited further, can be the most reasonable Choose and combine, thus meet reality application.
Compared with prior art, the invention has the beneficial effects as follows: 1, to be suitable for preparing lean leukocyte PRP white with richness for the present invention The multiple blood plasma such as cell PRP are centrifuged goods;2, the present invention is by preparing multiple PRP, and the indication of its PRP is more extensive, is suitable for In multiple diseases such as cartilage defect, osteoarthritis, wound surface, ligament injury, tendon injuries;3, the present invention use reasonably sealing, Aseptic design, use process is omnidistance without foreign substance is inserted the process of centrifuge tube, and the aseptic preparing blood product is more preferable;4、 When the present invention discharges erythrocyte with leukocyte, can at utmost reach to discharge effect, be visible by naked eyes residual, therefore can be at utmost Stop erythrocyte and enter final PRP, reduce interference, improve make efficiency;5, the present invention can be by the erythrocyte in preparation process, white The Fraction collection such as cell, blood plasma, is fed back in the patient, reduces patient and loses blood burden.
Accompanying drawing explanation
Fig. 1 is the structural representation of an embodiment of piston-type medical centrifugal of the present invention.
Detailed description of the invention
As it is shown in figure 1, the invention provides a kind of piston-type medical centrifugal, including centrifugal body 1 and described in being located at from Piston 2 within heart body 1;Wherein, described centrifugal its bottom is provided with nipple 3, and one end of described nipple 3 connects described centrifugal Body 1, the other end detachably connected nipple lid 4 and/or conversion mouth 5;Described piston 2 is arranged over external post 6, described external Post 6 is detachably connected with long handle 7.
In one embodiment, described piston-type medical centrifugal also includes all parts after depositing sterilization treatment Aseptic package bag.It is further preferred that it is medical at least to contain the two identical described piston-types of set in described aseptic package bag Centrifuge tube, is centrifuged the assemblies such as body 1, piston 2, nipple 3, external post 6, long handle 7, nipple lid 4, conversion mouth 5 and goes out through strictly Bacterium processes, and is placed in one.So, as members pollutes, the most do not affect normal use;By conversion mouth, can be by two Identical centrifugal body airtight connection, it is achieved internal liquid is pollution-free, the transfer of No leakage ground.
In an embodiment, described centrifugal body 1 make 5-100mL in the range of the utensil of specifications at different levels, with master scale Line.Different amounts of platelet rich plasma can be prepared according to different demands.
In an embodiment, the main part of described centrifugal body 1 is to be arranged above cylinder and bottom is passing through of symmetrical reverse taper Logical structure.This structure is conducive to blood constitutent to be enriched with in bottom, and the liquid height making same volume is higher, from described centrifugal When body discharges invalid component, can be more accurate.
In an embodiment, the high elastic rubber post of described piston 2 and described centrifugal body 1 contact internal walls, make described piston 2 Move up and down inside described centrifugal body 1 and play sealing function, so constant at piston position or when moving down, extraneous Antibacterial will not enter in the centrifuge tube body below piston;Described piston base is reverse taper, itself and the back taper of described centrifugal body Tightly it coincide bottom body, so, when piston is depressed into bottom, farthest blood constitutent can be released described centrifuge tube Body.
In an embodiment, described nipple 3 is divided into inside and outside two-layer, and its outer layer has screw thread or embedded ring, with described nipple lid 4 And the inside thread of described conversion mouth 5 matches, screw thread or embed can be formed and connect;Its internal layer is standard syringe nipple chi Very little, it is connected with all kinds of syringe needles, tube for transfusion;Wherein, the space of 0.5-1.5mm is left between described internal layer and described outer layer.This Sample, nipple lid is by nipple tight closure, and closes firmly, can bear the pressure in centrifugal process;Internal layer can connection standard syringe needle, Tube for transfusion, convenient platelet rich plasma preparation completed injects desired area, it is also possible to be directly connected to tube for transfusion, from patient's body Interior extraction blood;The space of inside and outside two interlayers allows tube for transfusion, the insertion of the first-class general purpose module of syringe needle.
In an embodiment, described external post 6 is external connection screw post, and it is located at the center above described piston 2, the most permissible Realize the effect removably connected well;The bottom of described long handle 7 is provided with nut, its internal whorl and described external connection screw post External screw-thread match, the upper end of described long handle 7 is the plane for push-and-pull, and the length of described long handle 7 more than described from The length of heart body 1, so when centrifugal, can take off long handle, prevent unavailable component stress, affect centrifuge results, non-from The heart period, by knob, long handle can be fixed on extraneous screw thread, push-and-pull piston, draw or get rid of the liquid in centrifuge tube body.
In an embodiment, described mouth 5 of changing is into hollow structure, and its one end is nut structure, the inside of described nut structure Screw thread outside screw thread and described nipple 3 matches;Its other end is needle hub size pipeline, screw thread or syringe needle, for Syringe nipple, tube for transfusion tail end airtight connection.So can realize the multifunction of the present invention, can by described conversion mouth, with Another identical described centrifugal body connects, it is possible to connect with other syringes or similar assembly.
Present invention also offers the preparation method of a kind of platelet rich plasma, medical centrifugal by piston-type described above Pipe is prepared.
In one embodiment, at least by any one piston-type medical centrifugal system that two sets are described above Standby.
Wherein in an embodiment, described preparation method uses the rich leukocyte PRP of secondary centrifuging method preparation, including following Step:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple, then will Long handle rotates from external post and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away Dynamic long handle 7, is expelled directly out the red blood cell layer of bottom from nipple 3 or is collected into asepsis injector by conversion mouth 5, in order to It is fed back in the patient;
Step 3: use nipple lid 4 closed rotary nipple 3, rotates long handle 7 from external post 6 and takes off, and recentrifuge is centrifugal complete After, long handle 7 is connected to external post 6, opens nipple lid 4, promote long handle 7, discharge remaining a small amount of red blood cell layer from nipple 3;
Step 4: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle 7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtains the Fu Baixi prepared Born of the same parents PRP.
In one embodiment, described preparation method uses secondary centrifuging method to prepare lean leukocyte PRP, including following step Rapid:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple 3, then will Long handle 7 rotates from external post 6 and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away Dynamic long handle 7, is expelled directly out red blood cell layer and the intermediate layer of bottom from nipple 3 or is collected into aseptic note by conversion mouth 5 In emitter, in order to be fed back in the patient;
Step 3: use nipple lid 4 closed rotary nipple 3, rotates long handle 7 from external post 6 and takes off, and recentrifuge is centrifugal complete After, long handle 7 is connected to external post 6, opens nipple 4, promote long handle 7, discharge remaining a small amount of red blood cell layer from nipple 3;
Step 4: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle 7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtain having prepared is lean white thin Born of the same parents PRP.
In one embodiment, centrifuging of described preparation method prepares lean leukocyte PRP, comprises the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple 3, then will Long handle 7 rotates from external post 6 and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away Dynamic long handle 7, is expelled directly out red blood cell layer and the intermediate layer of bottom from nipple 3 or is collected into aseptic note by conversion mouth 5 In emitter, in order to be fed back in the patient;
Step 3: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle 7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtain having prepared is lean white thin Born of the same parents PRP.
Piston-type medical centrifugal provided by the present invention can improve the convenience that PRP makes, it is possible to avoids completely behaviour The sample contamination occurred during work, can further improve platelet collection rate, reduces mixing of the impurity such as erythrocyte, leukocyte Miscellaneous, it is possible to collect different types of PRP according to different occupation modes.
Being described in detail the specific embodiment of the present invention above, but it is intended only as example, the present invention does not limit It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and Substitute the most all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and Amendment, all should contain within the scope of the invention.

Claims (10)

1. a piston-type medical centrifugal, it is characterised in that include centrifugal body and be located at the work of described centrifugal tubular body Plug;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, and the other end is detachable Ground connects nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post is detachably connected with long handle.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described piston-type medical centrifugal also includes The aseptic package bag of all parts after depositing sterilization treatment.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that on the main part of described centrifugal body is Side is communicating structure that cylinder and bottom are symmetrical reverse taper.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that the high elastic rubber post of described piston and institute State the contact of centrifugal inboard wall of tube body, make described piston move up and down at described centrifugal tubular body and play sealing function;Described work Being reverse taper bottom plug, it tightly coincide bottom the reverse taper of described centrifugal body.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described nipple is divided into inside and outside two-layer, outside it Layer has screw thread or embedded ring, matches with the inside thread of described nipple lid and described conversion mouth, can form screw thread or embedding Connect;Its internal layer is standard syringe teat dimensions, and it is connected with all kinds of syringe needles, tube for transfusion;Wherein, described internal layer and described outside The space of 0.5-1.5mm is left between Ceng.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described external post is external connection screw post, its It is located at the center above described piston;The bottom of described long handle is provided with nut, outside its internal whorl is with described external connection screw post Portion's screw thread matches, and the upper end of described long handle is the plane for push-and-pull, and the length of described long handle is more than described centrifugal body Length.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described mouth of changing into hollow structure, one End is for nut structure, and the screw thread outside the internal whorl of described nut structure and described nipple matches;Its other end is syringe needle Sleeve dimensions pipeline, screw thread or syringe needle, be used for and syringe nipple, tube for transfusion tail end airtight connection.
8. the preparation method of a platelet rich plasma, it is characterised in that by as described in any one in claim 1-7 Piston-type medical centrifugal is prepared.
The preparation method of platelet rich plasma the most according to claim 8, it is characterised in that at least by two sets such as right In requirement 1-7, the piston-type medical centrifugal described in any one is prepared.
The preparation method of platelet rich plasma the most according to claim 8, it is characterised in that comprise the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long Handle, is expelled directly out the red blood cell layer of bottom from nipple or is collected into asepsis injector by conversion mouth, in order to being fed back to In the patient;Or
Blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long handle, from Nipple is expelled directly out red blood cell layer and the intermediate layer of bottom or is collected in asepsis injector by conversion mouth, in order to return Transport in the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged, Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will Below blood intermediate layer in centrifugal body and plasma layer, component is injected in syringe, obtains the rich leukocyte PRP prepared Or lean leukocyte PRP.
CN201610597543.5A 2016-07-27 2016-07-27 A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma Pending CN106076448A (en)

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Cited By (8)

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CN106423321A (en) * 2016-12-07 2017-02-22 李众利 Centrifugal tube
CN106669875A (en) * 2017-02-13 2017-05-17 深圳市邦沃科技有限公司 Platelet-rich plasma centrifuge tube and extraction method thereof
CN109207355A (en) * 2018-10-15 2019-01-15 中国医学科学院整形外科医院 A kind of centrifuge tube
CN109439528A (en) * 2018-12-14 2019-03-08 中国人民解放军陆军特色医学中心 A kind of leucocyte sorting enriching apparatus and method
CN109865603A (en) * 2019-04-09 2019-06-11 广州医科大学附属第三医院 Integrated regeneration factor separation and concentration preparation facilities
CN110832061A (en) * 2017-10-06 2020-02-21 史蒂芬.何 Device and method for collecting and separating cells
CN113976335A (en) * 2021-10-29 2022-01-28 丁政 Centrifugal system for preparing leukocyte-removed platelet-rich plasma and preparation method thereof
CN114405069A (en) * 2022-01-28 2022-04-29 广东汉鼎医疗科技有限公司 PRP extraction device and implementation method thereof

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Application publication date: 20161109