CN106076448A - A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma - Google Patents
A kind of piston-type medical centrifugal and for preparing the preparation method of platelet rich plasma Download PDFInfo
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- CN106076448A CN106076448A CN201610597543.5A CN201610597543A CN106076448A CN 106076448 A CN106076448 A CN 106076448A CN 201610597543 A CN201610597543 A CN 201610597543A CN 106076448 A CN106076448 A CN 106076448A
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- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 19
- 210000000265 leukocyte Anatomy 0.000 claims abstract description 19
- 230000001954 sterilising effect Effects 0.000 claims abstract description 5
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- 210000002381 plasma Anatomy 0.000 claims description 11
- 238000000605 extraction Methods 0.000 claims description 8
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- 238000007789 sealing Methods 0.000 claims description 4
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Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
- B01L3/50825—Closing or opening means, corks, bungs
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- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- External Artificial Organs (AREA)
Abstract
The invention provides a kind of piston-type medical centrifugal, including centrifugal body and the piston being located at described centrifugal tubular body;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, the other end detachably connected nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post is detachably connected with long handle;Could be included for the aseptic package bag of all parts after depositing sterilization treatment.Present invention also offers the preparation method of a kind of platelet rich plasma, it is prepared by piston-type medical centrifugal described above.Piston-type medical centrifugal provided by the present invention can improve the convenience that PRP makes, the sample contamination occurred in operation can be avoided completely, can further improve platelet collection rate, reduce the doping of the impurity such as erythrocyte, leukocyte, it is possible to collect different types of PRP according to different occupation modes.
Description
Technical field
The present invention relates to a kind of medical apparatus and instruments, particularly relate to a kind of piston-type medical centrifugal and for preparing Fu Xue little
The preparation method of plate blood plasma.
Background technology
Platelet rich plasma (PRP) is isolatable from blood, containing transforming growth factor-β (transforming growth
Factor, TGF), platelet-derived growth factor (platelet-derived growth factor, PDGF), para-insulin
Somatomedin (insulin-like growth factor, IGF) and VEGF (vascular
Endothelial growth factor, VEGF) etc. multiple somatomedin, in addition, possibly together with leukocyte and fiber egg
In vain.Multiple studies have shown that, PRP is significant to soft tissue repair, it is possible to promotes regenerating bone or cartilage, slows down cartilage degeneration simultaneously.
Therefore, PRP has become the hot issue in current repair of cartilage field.
It is true that for the different tissues in human body, they are the most different for the requirement of the PRP used by reparation, such as rich white
Cell PRP is suitable for and most tissue, is but not suitable for using at intraarticular.Therefore, one can need to carry out according to difference
The equipment that strictly prepared by PRP is this area in the urgent need to.And current, pass through ten remainders PRP of U.S. FDA certification
Preparation equipment, except product prepare single, use dumb, extract the defects such as PRP mode is limited in addition to, there is also other various respectively
The deficiency of sample, number of times is loaded down with trivial details, platelet collection rate is low including expensive, centrifugal.And the similar product sold on domestic market
Product, there is also various limitation, if product design is that product is prepared single only in the product of wound;And owing to design is asked
Topic, blood is drawn and injects all by same suction pipe, thus remaining the impurity such as too much erythrocyte, thus affecting PRP system
Standby stability.
Summary of the invention
In order to solve the deficiencies in the prior art, the invention provides a kind of piston-type medical centrifugal and for preparing richness
The preparation method of thrombocyte plasma, the operation of described piston-type medical centrifugal is more convenient, preparation kind is more, range
More extensively, aseptic is higher.
Subject of the present invention is a kind of piston-type medical centrifugal, including centrifugal body be located at described centrifugal tubular body
Piston;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, and the other end can
Releasably connect nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post removably connects with long handle
Connect.
Preferably, described piston-type medical centrifugal also includes the aseptic bag of all parts after depositing sterilization treatment
Pack.It is further preferred that described aseptic package bag at least contains the two identical described piston-type medical centrifugals of set, from
The assemblies such as heart body, piston, nipple, external post, long handle, nipple lid, conversion mouth, through strictly sterilization treatment, are placed in one.
So, as members pollutes, the most do not affect normal use;By conversion mouth, can be by close for two identical centrifugal bodys
Close connection, it is achieved internal liquid is pollution-free, the transfer of No leakage ground.
Further, described centrifuge tube system become 5-100mL in the range of the utensil of specifications at different levels, with master scale line.
Different amounts of platelet rich plasma can be prepared according to different demands.
Further, the main part of described centrifugal body is to be arranged above cylinder and through knot that bottom is symmetrical reverse taper
Structure.This structure is conducive to blood constitutent to be enriched with in bottom, and the liquid height making same volume is higher, from described centrifugal body
During the invalid component of middle discharge, can be more accurate.
Further, the high elastic rubber post of described piston contacts with described centrifugal inboard wall of tube body, makes described piston in institute
State centrifugal tubular body move up and down and play sealing function, so constant at piston position or when moving down, extraneous antibacterial
Will not enter in the centrifuge tube body below piston;Described piston base is reverse taper, at the bottom of its reverse taper with described centrifugal body
Portion tightly coincide, and so, when piston is depressed into bottom, blood constitutent farthest can be released described centrifugal body.
Further, described nipple is divided into inside and outside two-layer, and its outer layer has screw thread or embedded ring, with described nipple lid and institute
The inside thread stating conversion mouth matches, and can form screw thread or embed connection;Its internal layer is standard syringe teat dimensions, its with
All kinds of syringe needles, tube for transfusion connect;Wherein, the space of 0.5-1.5mm is left between described internal layer and described outer layer.So, nipple
Lid is by nipple tight closure, and closes firmly, can bear the pressure in centrifugal process;Internal layer can connection standard syringe needle, tube for transfusion,
Convenient platelet rich plasma preparation completed injects desired area, it is also possible to be directly connected to tube for transfusion, from extracting in the patient
Blood;The space of inside and outside two interlayers allows tube for transfusion, the insertion of the first-class general purpose module of syringe needle.
Further, described external post is external connection screw post, and it is located at the center above described piston, so can be fine
Ground realizes the effect removably connected;The bottom of described long handle is provided with nut, outside its internal whorl is with described external connection screw post
Portion's screw thread matches, and the upper end of described long handle is the plane for push-and-pull, and the length of described long handle is more than described centrifugal body
Length, so when centrifugal, long handle can be taken off, prevent unavailable component stress, affect centrifuge results, in the non-centrifugal period,
By knob, long handle can be fixed on extraneous screw thread, push-and-pull piston, draw or get rid of the liquid in centrifuge tube body.
Further, described mouth of changing is into hollow structure, and its one end is nut structure, the internal whorl of described nut structure
Match with the screw thread outside described nipple;Its other end is needle hub size pipeline, screw thread or syringe needle, is used for and syringe
Nipple, tube for transfusion tail end airtight connection.So can realize the multifunction of the present invention, can be complete with another by described conversion mouth
Exactly the same described centrifugal body connects, it is possible to connect with other syringes or similar assembly.
The another one theme of the present invention is the preparation method of a kind of platelet rich plasma, by piston-type described above
Medical centrifugal is prepared.
Preferably, at least overlap any one piston-type medical centrifugal described above by two to be prepared.
Wherein in an embodiment, described preparation method uses the rich leukocyte PRP of secondary centrifuging method preparation, including following
Step:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle
To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from
Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long
Handle, is expelled directly out the red blood cell layer of bottom from nipple or is collected into asepsis injector by conversion mouth, in order to being fed back to
In the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged,
Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will
Below blood intermediate layer (rich in leukocyte, platelet) in centrifugal body and plasma layer, component is injected in syringe, is made
The standby rich leukocyte PRP completed.
In one embodiment, described preparation method uses secondary centrifuging method to prepare lean leukocyte PRP, including following step
Rapid:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle
To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from
Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long
Handle, is expelled directly out the red blood cell layer of bottom and intermediate layer (rich in leukocyte) from nipple or is collected into nothing by conversion mouth
In bacterium syringe, in order to be fed back in the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged,
Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will
Below blood intermediate layer (rich in platelet) in centrifugal body and plasma layer, component is injected in syringe, obtains having prepared
Lean leukocyte PRP.
In one embodiment, centrifuging of described preparation method prepares lean leukocyte PRP, comprises the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle
To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from
Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long
Handle, is expelled directly out the red blood cell layer of bottom and intermediate layer (rich in leukocyte) from nipple or is collected into nothing by conversion mouth
In bacterium syringe, in order to be fed back in the patient;
Step 3: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will
Below plasma layer in centrifugal body, component is injected in syringe, obtains the lean leukocyte PRP prepared.
It should be explicitly made clear at this point and above-mentioned the various of technical solution of the present invention are limited further, can be the most reasonable
Choose and combine, thus meet reality application.
Compared with prior art, the invention has the beneficial effects as follows: 1, to be suitable for preparing lean leukocyte PRP white with richness for the present invention
The multiple blood plasma such as cell PRP are centrifuged goods;2, the present invention is by preparing multiple PRP, and the indication of its PRP is more extensive, is suitable for
In multiple diseases such as cartilage defect, osteoarthritis, wound surface, ligament injury, tendon injuries;3, the present invention use reasonably sealing,
Aseptic design, use process is omnidistance without foreign substance is inserted the process of centrifuge tube, and the aseptic preparing blood product is more preferable;4、
When the present invention discharges erythrocyte with leukocyte, can at utmost reach to discharge effect, be visible by naked eyes residual, therefore can be at utmost
Stop erythrocyte and enter final PRP, reduce interference, improve make efficiency;5, the present invention can be by the erythrocyte in preparation process, white
The Fraction collection such as cell, blood plasma, is fed back in the patient, reduces patient and loses blood burden.
Accompanying drawing explanation
Fig. 1 is the structural representation of an embodiment of piston-type medical centrifugal of the present invention.
Detailed description of the invention
As it is shown in figure 1, the invention provides a kind of piston-type medical centrifugal, including centrifugal body 1 and described in being located at from
Piston 2 within heart body 1;Wherein, described centrifugal its bottom is provided with nipple 3, and one end of described nipple 3 connects described centrifugal
Body 1, the other end detachably connected nipple lid 4 and/or conversion mouth 5;Described piston 2 is arranged over external post 6, described external
Post 6 is detachably connected with long handle 7.
In one embodiment, described piston-type medical centrifugal also includes all parts after depositing sterilization treatment
Aseptic package bag.It is further preferred that it is medical at least to contain the two identical described piston-types of set in described aseptic package bag
Centrifuge tube, is centrifuged the assemblies such as body 1, piston 2, nipple 3, external post 6, long handle 7, nipple lid 4, conversion mouth 5 and goes out through strictly
Bacterium processes, and is placed in one.So, as members pollutes, the most do not affect normal use;By conversion mouth, can be by two
Identical centrifugal body airtight connection, it is achieved internal liquid is pollution-free, the transfer of No leakage ground.
In an embodiment, described centrifugal body 1 make 5-100mL in the range of the utensil of specifications at different levels, with master scale
Line.Different amounts of platelet rich plasma can be prepared according to different demands.
In an embodiment, the main part of described centrifugal body 1 is to be arranged above cylinder and bottom is passing through of symmetrical reverse taper
Logical structure.This structure is conducive to blood constitutent to be enriched with in bottom, and the liquid height making same volume is higher, from described centrifugal
When body discharges invalid component, can be more accurate.
In an embodiment, the high elastic rubber post of described piston 2 and described centrifugal body 1 contact internal walls, make described piston 2
Move up and down inside described centrifugal body 1 and play sealing function, so constant at piston position or when moving down, extraneous
Antibacterial will not enter in the centrifuge tube body below piston;Described piston base is reverse taper, itself and the back taper of described centrifugal body
Tightly it coincide bottom body, so, when piston is depressed into bottom, farthest blood constitutent can be released described centrifuge tube
Body.
In an embodiment, described nipple 3 is divided into inside and outside two-layer, and its outer layer has screw thread or embedded ring, with described nipple lid 4
And the inside thread of described conversion mouth 5 matches, screw thread or embed can be formed and connect;Its internal layer is standard syringe nipple chi
Very little, it is connected with all kinds of syringe needles, tube for transfusion;Wherein, the space of 0.5-1.5mm is left between described internal layer and described outer layer.This
Sample, nipple lid is by nipple tight closure, and closes firmly, can bear the pressure in centrifugal process;Internal layer can connection standard syringe needle,
Tube for transfusion, convenient platelet rich plasma preparation completed injects desired area, it is also possible to be directly connected to tube for transfusion, from patient's body
Interior extraction blood;The space of inside and outside two interlayers allows tube for transfusion, the insertion of the first-class general purpose module of syringe needle.
In an embodiment, described external post 6 is external connection screw post, and it is located at the center above described piston 2, the most permissible
Realize the effect removably connected well;The bottom of described long handle 7 is provided with nut, its internal whorl and described external connection screw post
External screw-thread match, the upper end of described long handle 7 is the plane for push-and-pull, and the length of described long handle 7 more than described from
The length of heart body 1, so when centrifugal, can take off long handle, prevent unavailable component stress, affect centrifuge results, non-from
The heart period, by knob, long handle can be fixed on extraneous screw thread, push-and-pull piston, draw or get rid of the liquid in centrifuge tube body.
In an embodiment, described mouth 5 of changing is into hollow structure, and its one end is nut structure, the inside of described nut structure
Screw thread outside screw thread and described nipple 3 matches;Its other end is needle hub size pipeline, screw thread or syringe needle, for
Syringe nipple, tube for transfusion tail end airtight connection.So can realize the multifunction of the present invention, can by described conversion mouth, with
Another identical described centrifugal body connects, it is possible to connect with other syringes or similar assembly.
Present invention also offers the preparation method of a kind of platelet rich plasma, medical centrifugal by piston-type described above
Pipe is prepared.
In one embodiment, at least by any one piston-type medical centrifugal system that two sets are described above
Standby.
Wherein in an embodiment, described preparation method uses the rich leukocyte PRP of secondary centrifuging method preparation, including following
Step:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even
It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple, then will
Long handle rotates from external post and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away
Dynamic long handle 7, is expelled directly out the red blood cell layer of bottom from nipple 3 or is collected into asepsis injector by conversion mouth 5, in order to
It is fed back in the patient;
Step 3: use nipple lid 4 closed rotary nipple 3, rotates long handle 7 from external post 6 and takes off, and recentrifuge is centrifugal complete
After, long handle 7 is connected to external post 6, opens nipple lid 4, promote long handle 7, discharge remaining a small amount of red blood cell layer from nipple 3;
Step 4: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle
7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtains the Fu Baixi prepared
Born of the same parents PRP.
In one embodiment, described preparation method uses secondary centrifuging method to prepare lean leukocyte PRP, including following step
Rapid:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even
It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple 3, then will
Long handle 7 rotates from external post 6 and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away
Dynamic long handle 7, is expelled directly out red blood cell layer and the intermediate layer of bottom from nipple 3 or is collected into aseptic note by conversion mouth 5
In emitter, in order to be fed back in the patient;
Step 3: use nipple lid 4 closed rotary nipple 3, rotates long handle 7 from external post 6 and takes off, and recentrifuge is centrifugal complete
After, long handle 7 is connected to external post 6, opens nipple 4, promote long handle 7, discharge remaining a small amount of red blood cell layer from nipple 3;
Step 4: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle
7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtain having prepared is lean white thin
Born of the same parents PRP.
In one embodiment, centrifuging of described preparation method prepares lean leukocyte PRP, comprises the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple 3 internal layer, by long handle 7 even
It is connected to external post 6, pulls back long handle 7, extract blood samples of patients, after extraction, use nipple lid 4 to close nipple 3, then will
Long handle 7 rotates from external post 6 and takes off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, long handle 7 is connected to external post 6, opens nipple lid 4, push away
Dynamic long handle 7, is expelled directly out red blood cell layer and the intermediate layer of bottom from nipple 3 or is collected into aseptic note by conversion mouth 5
In emitter, in order to be fed back in the patient;
Step 3: after discharge, rotates conversion mouth 5 and is fixed to nipple 3, is connected to syringe change mouth 5, promotes long handle
7, component below the blood intermediate layer in centrifugal body 1 and plasma layer is injected in syringe, obtain having prepared is lean white thin
Born of the same parents PRP.
Piston-type medical centrifugal provided by the present invention can improve the convenience that PRP makes, it is possible to avoids completely behaviour
The sample contamination occurred during work, can further improve platelet collection rate, reduces mixing of the impurity such as erythrocyte, leukocyte
Miscellaneous, it is possible to collect different types of PRP according to different occupation modes.
Being described in detail the specific embodiment of the present invention above, but it is intended only as example, the present invention does not limit
It is formed on particular embodiments described above.To those skilled in the art, any equivalent modifications that the present invention is carried out and
Substitute the most all among scope of the invention.Therefore, the impartial conversion made without departing from the spirit and scope of the invention and
Amendment, all should contain within the scope of the invention.
Claims (10)
1. a piston-type medical centrifugal, it is characterised in that include centrifugal body and be located at the work of described centrifugal tubular body
Plug;Wherein, described centrifugal its bottom is provided with nipple, and one end of described nipple connects described centrifugal body, and the other end is detachable
Ground connects nipple lid and/or conversion mouth;Described piston is arranged over external post, and described external post is detachably connected with long handle.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described piston-type medical centrifugal also includes
The aseptic package bag of all parts after depositing sterilization treatment.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that on the main part of described centrifugal body is
Side is communicating structure that cylinder and bottom are symmetrical reverse taper.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that the high elastic rubber post of described piston and institute
State the contact of centrifugal inboard wall of tube body, make described piston move up and down at described centrifugal tubular body and play sealing function;Described work
Being reverse taper bottom plug, it tightly coincide bottom the reverse taper of described centrifugal body.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described nipple is divided into inside and outside two-layer, outside it
Layer has screw thread or embedded ring, matches with the inside thread of described nipple lid and described conversion mouth, can form screw thread or embedding
Connect;Its internal layer is standard syringe teat dimensions, and it is connected with all kinds of syringe needles, tube for transfusion;Wherein, described internal layer and described outside
The space of 0.5-1.5mm is left between Ceng.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described external post is external connection screw post, its
It is located at the center above described piston;The bottom of described long handle is provided with nut, outside its internal whorl is with described external connection screw post
Portion's screw thread matches, and the upper end of described long handle is the plane for push-and-pull, and the length of described long handle is more than described centrifugal body
Length.
Piston-type medical centrifugal the most according to claim 1, it is characterised in that described mouth of changing into hollow structure, one
End is for nut structure, and the screw thread outside the internal whorl of described nut structure and described nipple matches;Its other end is syringe needle
Sleeve dimensions pipeline, screw thread or syringe needle, be used for and syringe nipple, tube for transfusion tail end airtight connection.
8. the preparation method of a platelet rich plasma, it is characterised in that by as described in any one in claim 1-7
Piston-type medical centrifugal is prepared.
The preparation method of platelet rich plasma the most according to claim 8, it is characterised in that at least by two sets such as right
In requirement 1-7, the piston-type medical centrifugal described in any one is prepared.
The preparation method of platelet rich plasma the most according to claim 8, it is characterised in that comprise the following steps:
Step 1: the injector end of the tube for transfusion equipment communicated with patient vessel is connected to nipple internal layer, connects long handle
To external post, pull back long handle, extract blood samples of patients, after extraction, use nipple lid to close nipple, then by long handle from
Rotate on external post and take off;
Step 2: blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long
Handle, is expelled directly out the red blood cell layer of bottom from nipple or is collected into asepsis injector by conversion mouth, in order to being fed back to
In the patient;Or
Blood samples of patients is layered after carrying out being centrifuged for the first time, and long handle is connected to external post, opens nipple lid, promotes long handle, from
Nipple is expelled directly out red blood cell layer and the intermediate layer of bottom or is collected in asepsis injector by conversion mouth, in order to return
Transport in the patient;
Step 3: use nipple lid closed rotary nipple, rotates long handle from external post and takes off, recentrifuge, after being centrifuged,
Long handle is connected to external post, opens nipple lid, promote long handle, discharge remaining a small amount of red blood cell layer from nipple;
Step 4: after discharge, rotates conversion mouth and is fixed to nipple, is connected to syringe change mouth, promotes long handle, will
Below blood intermediate layer in centrifugal body and plasma layer, component is injected in syringe, obtains the rich leukocyte PRP prepared
Or lean leukocyte PRP.
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Application publication date: 20161109 |