CN210301873U

CN210301873U - Cell separation suit

Info

Publication number: CN210301873U
Application number: CN201920187879.3U
Authority: CN
Inventors: 马超; 伍少玲; 栗晓; 刘翠翠; 范胜诺
Original assignee: Guangzhou Celeway Technology Co Ltd
Current assignee: Guangzhou Celeway Technology Co Ltd
Priority date: 2019-02-02
Filing date: 2019-02-02
Publication date: 2020-04-14
Anticipated expiration: 2029-02-02

Abstract

The utility model discloses a cell separation suit, including the centrifuging tube, install piston in the centrifuging tube, drive the gliding handle of piston in the centrifuging tube, the bottom of centrifuging tube is connected with the three-way valve, is equipped with vertical first export and horizontally second export on the three-way valve, still includes blood taking needle and pjncture needle, first exit linkage pjncture needle, second exit linkage blood taking needle. This cell separation suit connects the blood taking needle earlier and extracts human blood in the centrifuging tube, then tears the blood taking needle open, the centrifuging tube is arranged in centrifuge and is centrifuged, lower floor's erythrocyte after the centrifugation passes through the three-way valve and discharges to obtain rich platelet plasma in the centrifuging tube, the puncture needle is connected directly to the rethread three-way valve, it is human to pour into the rich platelet plasma in the centrifuging tube into, whole separation suit is compared with the special suit of PRP, moreover, the steam generator is simple in structure, the cost of manufacture is low, and can guarantee separation and treatment, this utility model is used for the medical instrument field.

Description

Cell separation suit

Technical Field

The utility model relates to the field of medical appliances, in particular to a cell separation set.

Background

The clinical medical value of autologous cells is continuously discovered and applied, and is becoming a new trend for treating diseases in clinical medicine. The concentration of platelets in Platelet Rich Plasma (PRP) is 3-5 times the concentration of platelets in normal whole blood; because the collagen can provide a microenvironment rich in a large number of growth factors and other cytokines, and promote the proliferation of cells, thereby promoting the healing of bone, tendon, ligament and soft tissue, the collagen is currently applied to a plurality of subjects such as orthopedics, stomatology, plastic surgery, rehabilitation and the like. The PRP preparation method comprises manual making and PRP special set making, and the current PRP making set has the defects of high price, difficult acquisition of materials and the like; however, many manual manufacturing methods require multiple liquid transfer, or the tools do not meet medical standards, the preparation process is complicated, the time consumption is long, and the concentration and the quality of platelets cannot be guaranteed.

SUMMERY OF THE UTILITY MODEL

The technical problem to be solved by the utility model is to provide a cell separation suit with low manufacturing cost, which can effectively separate blood cells to obtain platelet-rich plasma.

The technical scheme adopted for solving the technical problems is as follows:

the utility model provides a cell separation suit, includes the centrifuging tube, installs piston, the drive piston gliding handle in the centrifuging tube, and the bottom of centrifuging tube is connected with the three-way valve, is equipped with vertical first export and horizontally second export on the three-way valve, still includes blood taking needle and pjncture needle, first exit linkage pjncture needle, second exit linkage blood taking needle.

Preferably, the handle is removably connected to the piston.

Preferably, the blood taking device further comprises a connecting hose, wherein the second outlet of the three-way valve is connected with the connecting hose, and the blood taking needle is connected through the connecting hose.

Preferably, a syringe is further included, and the connection hose is connectable to the syringe.

Preferably, the junction of three-way valve and centrifuging tube is equipped with the filter screen.

Preferably, the centrifugal tube separator further comprises an isolation sleeve capable of wrapping the centrifugal tube.

Preferably, the puncture needle comprises a first puncture needle for injection into the hip joint and a second puncture needle for injection into the shoulder or knee joint.

Preferably, the capacity of the centrifugal tube is 10-30 ml, and the outer wall of the centrifugal tube is provided with scale marks.

The utility model has the advantages that: this cell separation suit connects the blood taking needle earlier and extracts human blood in the centrifuging tube, then tears the blood taking needle open, and the centrifuging tube is arranged in centrifuge and is centrifuged, and lower floor's erythrocyte after centrifugation passes through the three-way valve and discharges to obtain rich platelet plasma in the centrifuging tube, rethread three-way valve lug connection pjncture needle, it is human to pour into the rich platelet plasma in the centrifuging tube into, and whole separation suit is compared with the special suit of PRP, simple structure, and the cost of manufacture is low, and can guarantee separation and treatment.

Drawings

The present invention will be further explained with reference to the accompanying drawings:

fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a schematic structural diagram of all devices encapsulated in the sealing bag according to the embodiment of the present invention.

Detailed Description

Referring to fig. 1 and 2, the utility model relates to a cell separation suit, including centrifuging tube 1, install piston 2 in centrifuging tube 1, drive piston 2 gliding handle 3 in centrifuging tube 1, centrifuging tube 1's bottom is connected with three-way valve 4, is equipped with vertical first export 41 and horizontally second export 42 on three-way valve 4, still includes blood taking needle 5 and pjncture needle 6, and pjncture needle 6 is connected to first export 41, and blood taking needle 5 is connected to second export 42.

This cell separation suit, mainly used separation blood cell is in order to prepare rich platelet plasma, connects blood taking needle 5 earlier and draws human blood in centrifuging tube 1, then tears blood taking needle 5 open, and centrifuging tube 1 is arranged in the centrifuge and is centrifuged, and the lower floor's erythrocyte after the centrifugation passes through three-way valve 4 and discharges to obtain rich platelet plasma in centrifuging tube 1, 4 lug connection pjncture needles 6 of rethread three-way valve, it is human to pour into the rich platelet plasma in centrifuging tube 1. Compared with the PRP special suit, the whole separation suit has simple structure and low manufacturing cost, and can ensure the separation and treatment effects.

Preferably, the handle 3 is detachably connected to the piston 2, in particular, a screw connection can be used. The handle 3 drives the piston 2 to slide in the centrifuge tube 1, so that the blood drawing of the centrifuge tube 1 and the injection of platelet-rich plasma in the centrifuge tube 1 into a human body can be realized. During centrifugation, tear handle 3 open to reduce centrifuge tube 1's whole height dimension, save space, arrange centrifuge tube 1 in centrifuge's centrifugal sleeve again, be convenient for placing and operating in the centrifuge. Alternatively, the handle 3 is divided into an upper section and a lower section, the upper section and the lower section are detachably connected, and a threaded connection or a bayonet connection can be adopted.

Preferably, the blood collection device further comprises a connection hose 7, the second outlet 42 of the three-way valve 4 is connected with the connection hose 7, and the blood collection needle 5 is connected through the connection hose 7. The blood taking needle 5 is connected with the connecting hose 7 to facilitate blood taking because the hose of the blood taking needle 5 is short.

Preferably, a syringe 8 is further included, and the connection hose 7 can be connected with the syringe 8. After the blood sampling is completed, the blood taking needle 5 is detached, then after the centrifugation is completed, the second outlet 42 of the three-way valve 4 is connected with the injector 8 through the connecting hose 7, and the injector 8 is used for extracting lower layer red blood cells in the centrifugal tube 1 after the centrifugation, so that the lower layer red blood cells are separated out.

Preferably, a filter screen 9 is arranged at the joint of the three-way valve 4 and the centrifuge tube 1, specifically, the pore size of the filter screen 9 is 0.22 μm, which is used for filtering bacteria and ensuring sterility.

Preferably, the centrifugal tube centrifuge device further comprises an isolation sleeve 12 capable of wrapping the centrifugal tube 1, and the isolation sleeve 12 is sleeved on the centrifugal tube 1 when the centrifugal tube 1 is placed in the centrifuge for centrifugation, so that the centrifugal tube 1 is prevented from being polluted during each centrifugation.

Preferably, the puncture needle 6 comprises a first puncture needle for hip joint injection and a second puncture needle for shoulder joint or knee joint injection. When the injection is injected into hip joints of a human body, a first puncture needle is connected, and the specification of the first puncture needle is 0.7mm in diameter and 90mm in length; when the puncture needle is injected into shoulder joints or knee joints of a human body, the puncture needle is connected with a second puncture needle, and the specification of the second puncture needle is 0.7mm in diameter and 38-50 mm in length.

Preferably, the capacity of the centrifugal tube 1 is 10-30 ml, and the outer wall of the centrifugal tube 1 is provided with scale marks 11, so that an operator can accurately master the volume of blood extraction and lower layer red blood cell separation and removal in the process of preparing platelet-rich plasma, and the accuracy of the preparation process is improved.

As shown in FIG. 2, all the devices of the present cell separation set are enclosed in a sealing bag 13.

The following specific steps for preparing platelet rich plasma by the cell separation kit are as follows:

(1) mixing anticoagulant with autologous blood of a patient according to a ratio of 1:9, connecting a connecting hose 7 with a second outlet 42 of a three-way valve 4, connecting the connecting hose 7 with a blood taking needle 5, pulling up a handle 3, drawing the blood into a centrifuge tube 1 through the blood taking needle 5 with a drawing amount of 10ml, and then removing the connecting hose 7 and the blood taking needle 5;

(2) removing the handle 3, sleeving the centrifugal tube 1 with the isolation sleeve 12, and then placing the centrifugal tube in a centrifuge to rotate at the rotating speed of 1200-1600 rpm for 8-12 min;

(3) taking out the centrifuge tube 1, installing the handle 3, connecting the second outlet 42 of the three-way valve 4 with the connecting hose 7, connecting the connecting hose 7 with the injector 8, pressing down the handle 3, and extracting the lower layer red blood cells in the centrifuge tube 1 by the injector 8;

(4) removing the handle 3, sleeving the centrifugal tube 1 with the isolation sleeve 12, placing the centrifugal tube in the centrifuge again for secondary centrifugation, and rotating at the rotating speed of 1800-2200 rpm for 8-12 min;

(5) taking out the centrifuge tube 1, installing the handle 3, connecting the second outlet 42 of the three-way valve 4 with the connecting hose 7, connecting the connecting hose 7 with the injector 8, pressing down the handle 3, pumping out the lower layer red blood cells in the centrifuge tube 1 again by the injector 8, and leaving 3-4 ml of platelet-rich plasma at the bottom of the centrifuge tube 1;

(6) the first outlet 41 of the three-way valve 4 is connected with the puncture needle 6 and directly injected into the joint part of the human body.

Except the centrifugation in the centrifuge, other steps are performed under the principles of super clean bench and strict aseptic operation, so that sample pollution in the operation process can be avoided to a great extent. The whole preparation process and injection can be carried out in the same centrifugal tube 1 without repeated transfer, thereby avoiding infection, ensuring the concentration of the blood platelet and facilitating the injection operation. Compared with the PRP special suit, the separating suit has simple structure and low cost, and can ensure the separating and treating effects.

In this document, the terms front, back, upper and lower are used to define the components in the drawings and the positions of the components relative to each other, and are used for clarity and convenience of the technical solution. It is to be understood that the use of the directional terms should not be taken to limit the scope of the claims.

The features of the embodiments and embodiments described herein above may be combined with each other without conflict.

Of course, the invention is not limited to the above-mentioned embodiments, and those skilled in the art can make equivalent modifications or substitutions without departing from the spirit of the invention, and these equivalent modifications or substitutions are included in the scope defined by the claims of the present application.

Claims

1. A cell separation kit, comprising: including centrifuging tube, install piston, the drive in the centrifuging tube the gliding handle of piston in the centrifuging tube, the bottom of centrifuging tube is connected with the three-way valve, be equipped with vertical first export and horizontally second export on the three-way valve, still include blood taking needle and pjncture needle, first exit linkage pjncture needle, second exit linkage blood taking needle.

2. The cell separation kit of claim 1, wherein: the handle is detachably connected with the piston.

3. The cell separation kit of claim 1, wherein: still include coupling hose, the second exit linkage of three-way valve coupling hose passes through coupling hose connects the blood taking needle.

4. The cell separation kit of claim 3, wherein: also comprises a syringe, and the connecting hose can be connected with the syringe.

5. The cell separation kit of claim 1, wherein: and a filter screen is arranged at the joint of the three-way valve and the centrifugal tube.

6. The cell separation kit of claim 1, wherein: still include the isolation sleeve that can wrap up the centrifuging tube.

7. The cell separation kit of claim 1, wherein: the puncture needle comprises a first puncture needle for injecting the hip joint and a second puncture needle for injecting the shoulder joint or the knee joint.

8. The cell separation kit of any one of claims 1 to 7, wherein: the capacity of the centrifugal tube is 10-30 ml, and the outer wall of the centrifugal tube is provided with scale marks.