CN210384934U - Preparation facilities of rich platelet plasma - Google Patents

Abstract

The utility model provides a preparation device of platelet-rich plasma, which comprises a liquid transfer component and a centrifugal component; the liquid transferring part is used for injecting or extracting liquid, and the centrifugal part is used for centrifuging the liquid to prepare the platelet-rich plasma; wherein, centrifugation part includes at least one centrifuging tube, the centrifuging tube include the body with move about set up in cursory in the body, cursory with the body pastes and can follow in the wall the axis direction of body slides it is equipped with the passageway to float in, the passageway includes first port and second port, first port with the entry of centrifuging tube is passed through the hose and is linked together. The device of an embodiment of the utility model has a simple structure and can be used for preparing platelet-rich plasma.

Description

Preparation facilities of rich platelet plasma

Technical Field

The utility model relates to a preparation of plasma, in particular to a preparation device of platelet-rich plasma.

Background

Platelet Rich Plasma (PRP) is obtained by centrifuging autologous venous whole blood, and contains high concentration of platelets, and the concentrated platelets can release derivative growth factor (PDGF), transforming growth factor- β (TGF- β), insulin-like growth factor (IGF), Epidermal Growth Factor (EGF), Vascular Endothelial Growth Factor (VEGF), Fibroblast Growth Factor (FGF) and the like in an activated state, and the growth factors have the effects of controlling inflammation, promoting cell and tissue regeneration and reducing the formation of postoperative scars.

In addition, due to abundant sources, convenient material acquisition and simple preparation method, the PRP is widely applied to operations of orthopedics, cardiac surgery, cosmetic plastic, sports medicine and the like. However, existing imported or domestic PRP preparation kits typically consist of a multi-chamber threaded structure, which often results in difficult handling and precise access to the isolated PRP due to poor visibility during PRP preparation, and, in addition, such kits are prone to inadvertent platelet aggregation.

SUMMERY OF THE UTILITY MODEL

The utility model has a main purpose of providing a preparation device of platelet-rich plasma, which comprises a liquid transferring component and a centrifugal component; the liquid transferring part is used for injecting or extracting liquid, and the centrifugal part is used for centrifuging the liquid to prepare the platelet-rich plasma; wherein, centrifugation part includes at least one centrifuging tube, the centrifuging tube include the body with move about set up in cursory in the body, cursory with the body pastes and can follow in the wall the axis direction of body slides it is equipped with the passageway to float in, the passageway includes first port and second port, first port with the entry of centrifuging tube is passed through the hose and is linked together.

According to the utility model discloses an embodiment, the cursory is a cylinder, the passageway is followed the direction of height of cylinder is seted up, the passageway is round platform shape, the height of passageway equals the height of cylinder, first port is located the first bottom surface of cylinder, the second port is located the second bottom surface of cylinder, the diameter of first port is less than the diameter of second port.

According to the utility model discloses an embodiment, the lateral wall of passageway with the contained angle of second bottom surface is 30 ~ 60 degrees.

According to the utility model discloses an embodiment, the lateral wall of passageway with the contained angle of second bottom surface is 45 degrees.

According to the utility model discloses an embodiment, the centrifuging tube including set up respectively in the first sealed lid and the sealed lid of second at body both ends.

According to the utility model discloses an embodiment, the entry is seted up in first sealed lid the air vent has still been seted up on the first sealed lid.

According to an embodiment of the present invention, the vent hole is provided with a sterile filter membrane.

According to an embodiment of the present invention, a mark for marking a liquid interface position is provided on the side wall of the pipe body.

According to the utility model discloses an embodiment, the device includes the centrifuge tube cover, the centrifuge tube cover includes sleeve pipe, cover pipe lid and two elastomers, the sleeve pipe is used for holding the centrifuging tube, during the use the centrifuging tube holds in the sleeve pipe and is located between two elastomers.

According to the utility model discloses an embodiment, sleeve pipe and casing cover interference fit, the elastomer with the casing cover is connected.

The device of an embodiment of the utility model has a simple structure and can be used for preparing platelet-rich plasma.

Drawings

The various objects, features and advantages of the present invention will become more apparent from the following detailed description of the preferred embodiments of the invention, when considered in conjunction with the accompanying drawings. The drawings are merely exemplary of the invention and are not necessarily drawn to scale. In the drawings, like reference characters designate the same or similar parts throughout the different views. Wherein:

fig. 1 is a schematic structural diagram of a centrifuge tube according to an embodiment of the present invention;

fig. 2 is a schematic structural view of a centrifuge tube in a centrifuge tube sleeve according to an embodiment of the present invention.

Detailed Description

Exemplary embodiments that embody features and advantages of the present invention will be described in detail in the following description. It is to be understood that the invention is capable of other and different embodiments and its several details are capable of modification without departing from the scope of the invention, and that the description and drawings are to be regarded as illustrative in nature and not as restrictive.

The utility model provides a preparation device of platelet-rich plasma, which comprises a liquid transfer component and a centrifugal component; the liquid transferring part is used for injecting or extracting liquid, and the centrifugal part is used for centrifuging the liquid to prepare platelet-rich plasma; the centrifugation component may include a centrifuge, a centrifuge tube, or the like.

In one embodiment, the centrifuge component comprises at least one centrifuge tube, for example two centrifuge tubes, which may be a straight barrel type single chamber centrifuge tube.

In one embodiment, the pipetting means may be a syringe.

In one embodiment, as shown in fig. 1, the centrifugal tube 10 includes a tube 11, the tube 11 is a cylindrical body, such as a cylinder, and an upper cap 12 (a first sealing cover) and a lower cap 13 (a second sealing cover) are respectively disposed at two ends of the tube 11 to enable the tube 11 to be in a sealing state.

In one embodiment, the upper cap 12 and the lower cap 13 are interference fit with the tube 11, or connected by gluing, ultrasonic welding, or the like.

In another embodiment, the upper cap 12, the lower cap 13 and the tube 11 may be integrally formed by an injection molding process or an extrusion molding process.

In one embodiment, an inlet 121 is formed on the upper cap 12, the inlet 121 is used to provide an interface for the pipetting device, and a cover can be disposed on the inlet to close the inlet.

In one embodiment, the upper cap 12 is provided with a vent hole 122, a sterile filter membrane can be disposed in the vent hole 122 to prevent a contamination source from entering the tube 11, and a hole cover is disposed on the vent hole 122 and can be opened during centrifugation.

In one embodiment, the tube 11 is transparent or translucent (made of transparent material) so as to observe the state of the liquid in the tube 11. The sidewall of the tube 11 may be provided with a mark 15 for identifying the interface position of the centrifuged liquid in the tube 11 or the portion of the liquid that should be extracted, and the mark 15 may be provided in different colors and shapes, and may be one or more mark lines, such as mark lines b1, a, b2 shown in fig. 1.

In one embodiment, a float 14 is provided in the tube 11, the float 14 communicates with the inlet of the centrifugal tube 10, and the liquid transfer member can inject the raw material liquid into the tube 11 through the float 14.

In one embodiment, the float 14 is disposed on the tube wall of the tube 11 and can slide up and down (along the axial direction of the tube 11) along the tube wall of the tube 11, the float 14 can divide the interior of the tube 11 into an upper cavity and a lower cavity, and the liquid to be centrifuged is located in the lower cavity.

In one embodiment, the float 14 is shaped to match the shape of the tube 11. For example, the tube 11 is a circular tube, and the float 14 is a cylinder with a diameter equal to or slightly smaller than the inner diameter of the tube 11, so that it can be tightly attached to the inner wall of the tube 11 and can be slidably disposed in the tube 11. Because the buoy 14 is tightly attached to the pipe wall, in the process of sliding up and down along the pipe wall, liquid below the buoy 14 can be prevented from entering the cavity above the buoy 14 to the greatest extent.

In one embodiment, a passage 141 is formed in the float 14, and the passage 141 is connected to the inlet 121 of the upper cap 12.

In one embodiment, the channel 141 is in the shape of a truncated cone with a bottom opening larger than a top opening, and the channel 141 is connected to the inlet 121 through its top opening, preferably, the channel 141 is connected to the inlet through a hose.

In one embodiment, the float 14 is a cylinder; the channel 141 is in a truncated cone shape and is opened along the height direction of the cylinder, and the height of the channel 141 is the same as the height of the cylinder, that is, the upper port of the channel 141 is located on the upper bottom surface of the cylinder, the lower port is located on the lower bottom surface of the cylinder, the diameter of the upper port is smaller than that of the lower port, and preferably, the diameter of the lower port of the channel 141 is equal to the inner diameter of the cross section of the cylinder.

The utility model relates to an embodiment's device, owing to adopt the cursory structure of taking the cone angle, and the centrifugation pipe wall is pressed close to the one end of cursory 14 interior passageways, can steadily acquire the PRP who separates accurately.

In one embodiment, the disturbance of the liquid below the float 14 can be reduced by drawing liquid from the cavity below the float 14 by placing a pipetting member, such as a syringe, in communication with the inlet 121 and injecting liquid into the cavity below the float 14 or drawing liquid from the cavity below the float 14 through the hose and channel 141.

In one embodiment, as shown in fig. 2, a centrifuge tube housing can be disposed outside the centrifuge tube 10, the centrifuge tube housing comprises a sleeve 21, two sleeve covers 22 and two elastic bodies 23, the sleeve 21 has a hollow space for accommodating the centrifuge tube 10. An elastic body 23 is arranged inside the sleeve 21, the centrifuge tube 10 can be arranged in the centrifuge sleeve before centrifugation, the centrifuge tube is fixed by pressing the sleeve cover 22, and the centrifuge tube 10 is positioned between the two elastic bodies 23. In the centrifugation and operation processes, the elastic body 23 can reduce the disturbance of deceleration inertia and other external forces to the liquid in the centrifugal tube 10, and the stability of the liquid layering interface after centrifugation is kept.

In one embodiment, the two elastic bodies 23 are not connected to the sleeve 21 and the sleeve cover 22, and when the centrifuge tube 10 is used, the two elastic bodies 23 are sandwiched between the two bottom surfaces of the centrifuge tube 10 and the sleeve 21 under the pressure of the centrifuge tube 10.

In one embodiment, two elastomers 23 are attached to each of the two cannula caps 22.

The utility model relates to an embodiment's device through adopting pipe-in-pipe structure and setting up damping part, can reduce acceleration and atress when centrifugal start and brake to avoid the platelet to produce and condense.

In one embodiment, the raw material solution for centrifugation may be a mixture of whole blood and an anticoagulant.

Hereinafter, a platelet-rich plasma production apparatus and a method of using the same according to an embodiment of the present invention will be described in detail.

(1) Adding 10 parts by volume of ACD-A anticoagulant into a disposable sterile syringe, pushing and pulling a syringe push rod to enable the anticoagulant to be hung on the inner wall of a syringe sleeve, extracting 90 parts by volume of venous blood of a recipient, and mixing the venous blood with the anticoagulant uniformly.

(2) Opening the inlet cover and the hole cover of the centrifuge tube 10, connecting the lower end of the syringe with the channel 141 through the inlet 121 of the upper cap 12, and injecting the venous blood containing the anticoagulant into the centrifuge tube 10 with the float 14 through the channel 141; after that, the centrifuge tube 10 is inserted into the sleeve 21 between the two elastic bodies 23, and the sleeve cover 22 is pressed to fix the centrifuge tube 10.

(3) And (3) placing another same centrifugal tube into a low-speed centrifuge together after balancing for primary centrifugation, wherein the specific centrifugation conditions are as follows: the raw material liquid in the centrifuge tube was separated into two layers, i.e., a yellow transparent layer (upper layer) and a red turbid layer (lower layer) by centrifugation at 605 × g for 10 minutes under centrifugal force, and the height ratio of the yellow transparent layer to the red turbid layer was about 55: 45, and the interface between the two layers of liquid was located substantially at mark a in the centrifuge tube wall mark 15.

(4) Extract and abandon the upper two-thirds of yellow stratum lucidum with disposable aseptic syringe, then extract the lower third of yellow stratum lucidum and the upper third of red turbid layer and mix the back, pour into the mixed liquid in the second centrifuging tube to place this centrifuging tube in the centrifuging tube cover, carry out the secondary centrifugation, specific centrifugation condition is: centrifuging for 8 minutes by 823 Xg centrifugal force, separating the mixed solution in the centrifuge tube into three layers from top to bottom, namely a PPP (platelet poor plasma) layer, a PRP (platelet rich plasma) layer, a leukocyte layer and a red blood cell layer, extracting by using a disposable sterile syringe, discarding most of the PPP layer, reserving about 2 mm of the PPP layer at the lower part (namely the PPP layer which is adjacent to the upper part of the PRP layer and the leukocyte layer), extracting the PPP layer of about 2 mm to the red blood cell layer of about 1 mm below the leukocyte layer, and uniformly mixing to obtain the platelet rich plasma.

Unless otherwise defined, all terms used in the present invention have the meanings commonly understood by those skilled in the art.

The described embodiments are for illustrative purposes only and are not intended to limit the scope of the present invention, and various other substitutions, changes and modifications may be made by those skilled in the art within the scope of the present invention.

Claims (10)

1. A device for preparing platelet rich plasma, comprising:

a pipetting member for injecting or extracting a liquid; and

a centrifugation section for subjecting a liquid to a centrifugation process to prepare the platelet-rich plasma;

wherein, centrifugation part includes at least one centrifuging tube, the centrifuging tube include the body with move about set up in cursory in the body, cursory with the body pastes and can follow in the wall the axis direction of body slides it is equipped with the passageway to float in, the passageway includes first port and second port, first port with the entry of centrifuging tube is passed through the hose and is linked together.

2. The device of claim 1, wherein the float is a cylinder, the channel is formed along the height direction of the cylinder, the channel is in a truncated cone shape, the height of the channel is equal to the height of the cylinder, the first port is located on a first bottom surface of the cylinder, the second port is located on a second bottom surface of the cylinder, and the diameter of the first port is smaller than that of the second port.

3. The device of claim 2, wherein the side wall of the channel forms an angle of 30-60 degrees with the second bottom surface.

4. The device of claim 2, wherein the side wall of the channel is angled at 45 degrees from the second bottom surface.

5. The apparatus of claim 1, wherein the centrifuge tube comprises a first sealing cap and a second sealing cap disposed at two ends of the tube body, respectively.

6. The apparatus of claim 5, wherein the inlet is disposed in the first sealing cap, and a vent is disposed in the first sealing cap.

7. The device of claim 6, wherein a sterile filter is disposed over the vent.

8. The device of claim 1, wherein indicia for identifying the location of the liquid interface is provided on the sidewall of the tube.

9. An apparatus according to claim 1, comprising a centrifuge tube housing comprising a sleeve for receiving the centrifuge tube, a sleeve cap and two resilient bodies, the centrifuge tube being received within the sleeve and located between the two resilient bodies in use.

10. The device of claim 9, wherein the cannula has an interference fit with a cannula cover, the elastomer being coupled to the cannula cover.

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