TWM483797U - Device for separating blood components - Google Patents

Description

Blood separation device

The present invention relates to a blood separation device, and more particularly to a device for stratifying blood in a centrifuge based method for separating and collecting a specific plasma layer.

The blood is rich in a variety of ingredients and the functions of the different ingredients vary. Traditionally, the peripheral blood obtained directly by blood sampling is "whole blood", which contains blood cells, serum, plasma and a large number of biochemical molecules, and different components have different medical uses. The separation of whole blood is a very common practice in today's medical practice. Among them, the centrifugation method is a very common separation method; by centrifugation, the blood components are layered by the difference in specific gravity, thereby obtaining the desired blood components.

In general, after obtaining the blood of the patient and performing anticoagulant treatment, the blood can be stratified by its specific gravity by centrifugation, and the upper layer of plasma and the lower layer of red blood cell sediment can be clearly observed, wherein the upper layer of plasma is roughly On the other hand, it is based on the small amount of platelet-poor plasma (PPP) and the plate-rich plasma (PRP). There is a special plasma layer called "buffy coat" between the upper plasma and the red blood cell sediment, which is still a part of the platelet-rich plasma in a broad sense. However, the buffy coat is compared. The volume of the buffy coat in the upper layer of plasma or the lower layer of red blood cells is relatively small, accounting for only about 1% of the total blood volume, and thus exhibits a thin layer in the stratified blood.

Among the above blood components, the medical value of the plasma fraction has been gradually discovered and paid attention to in recent years. Specifically, autologous platelet-rich plasma (for the sake of brevity, the following is abbreviated by the PRP description) and the buffy coat are widely used for medical purposes, and have a medical effect and high safety. Many advantages. In particular, the buffy coat is enriched with high concentrations of platelets, lymphocytes, growth factors, and a small amount of stem cells.

It is known that platelets in PRP can secrete abundant and diverse growth factors after appropriate stimulation, and corresponding to initiate biochemical mechanisms, triggering various physiological repair mechanisms such as proliferation, movement, differentiation and collagen synthesis; on the other hand, buffy brown The layer also contains a small amount of precious adult stem cells, hematopoietic stem cells and mesenchymal stem cells, and in the above-mentioned platelet and growth factor environment, stem cells can also participate in and promote tissue repair after activation. The research and application of PRP has become an important field of regenerative medicine. PRP can promote tissue repair function has been widely studied and practically applied to clinical medicine, which is mainly used to promote wound healing and tissue repair, such as: dental treatment, orthopedic treatment, ophthalmic treatment, arthritis treatment, plastic surgery, beauty Medical and ophthalmic treatments have deep application value.

At present, the method adopted for PRP is still mainly based on centrifugal tube extraction. However, the proportion of PRP in the overall blood volume is relatively small due to the stratified centrifugation of blood. Especially according to the general blood volume (10 to 20 mL of whole blood), only a thin layer of buffy coat can be obtained after centrifugation, which makes it difficult for the operator to distinguish and separate the buffy coat layer from the plasma layer and the red blood cell layer; In addition, the thin layer of buffy coat is between the plasma layer and the red blood cell layer, and it is necessary to take tools to extract it in the operation, which significantly interferes with the blood stratification effect and the operation efficiency is poor; Blood buffy brown layer. In addition to the problem of inconvenient operation, the extraction of PRP or buffy coat by centrifugation involves a number of complicated separation procedures, in particular, in order to separate the stratified blood, multiple pipetting procedures are required, which also leads to contamination concerns and infection risks. Security issues.

Therefore, some manufacturers currently propose improvements to the aforementioned related issues. For example, Chinese Patent No. CN202478263U and CN201469752U propose a very similar improvement method. Both methods adopt a conventional syringe as a blood drawing device, remove the injection needle after taking blood, and use a container with a piston. And sleeved to the opening of the syringe (ie, the original injection needle socket), and then the assembled syringe is centrifuged together with the container sleeved thereon, and the syringe is placed on the top and the container is placed under the centrifuge; Therefore, the plasma after centrifugation is collected by discharging the blood layer at the bottom downward into the container to lower the PRP to the opening of the syringe, and then installing the injection needle to directly inject the PRP back into the blood-derived patient.

However, in terms of actual operation, there are still many defects in the devices described in the above two patents to be improved. Specifically, according to the design disclosed in the above two patents, although the existing syringe can be used as the blood drawing and injection device, the syringe and the attached container are connected in a socket manner, and both are long tubular members. Therefore, the overall device is still in a very unstable state after assembly, especially the possibility of loosening the socket; further, it is limited by the principle of device design and centrifugal separation of blood, and the device must be centrifuged. The syringe and the container are assembled so that the two members are collectively centrifuged. Therefore, the structure of the device is unstable as described above, and the centrifugal force is inevitably subjected to a strong force, which is more likely to cause loosening, and once the device is loosely structured It is more likely to cause serious safety problems such as blood leakage and pollution. In addition, since the use of the foregoing device for obtaining PRP, in order not to interfere with the effect of stratification of the centrifuged blood, it is necessary to inject a hematosphere having a specific gravity larger than PRP downward, and to keep the PRP at the proximal exit of the syringe by artificial control; The operation of discharging the liquid downward is affected by gravity, and the operation of the disturbance is not easy, and the PRP may be accidentally discharged to reduce the extraction effect; further, if the thin layer of the buffy coat is separated and extracted, the above The lack will be more significant.

On the other hand, U.S. Patent Application Publication No. 20100025342A1 proposes a blood separation device which is also based on the principle of a conventional syringe, which employs two syringes, one of which is a first syringe for mounting an injection needle and extracting Whole blood, after the whole blood is obtained, the injection needle is replaced by a cap to seal the opening, and then the injection opening is arranged downward, a weak centrifugal procedure is applied to initially separate the blood, and then, with the injection needle The second syringe is placed in the conduit reserved by the push handle of the first syringe, and the majority of the initially separated plasma is aspirated (ie, the initial liquid is obtained by preliminary centrifugation); then the injection needle is replaced by another cap, and the second syringe is replaced. The opening is sealed, followed by strong centrifugation to facilitate more precise stratification of the plasma and to vent a specific blood layer down (ie, in the direction of its gravity) to obtain a PPR or buffy coat.

However, in view of the technical solution disclosed in the above U.S. Patent Application, the blood separation device design principle also uses a conventional syringe, and at least two syringes must be used for the purpose. Therefore, in terms of practical operation, in order to accurately insert the second syringe into the first syringe and achieve the goal of stably absorbing stratified blood, the size and configuration of the two syringes must be additionally designed so that the two syringes can Stable assembly, so the two syringes must not be directly achieved with the existing specifications of the syringe, and a single blood separation requires the use of two syringes, significantly increasing production costs. Furthermore, in terms of function, although the device may improve the blood separation effect, the operation involves a two-stage centrifugation and pipetting step, so that the problem of the complicated process is still not solved; and the syringe is sleeved during the separation process. The steps may also lead to concerns about operational pollution. In addition, the fin design, which is limited by the end of the conventional syringe sleeve, limits the placement of the device during centrifugation (the presence of the flap prevents the conventional syringe from being placed in a conventional centrifuge tube) and must be used downwards. The injection method obtains the PRP, and the cap used for the sealing is in the direction of the applied force during centrifugation, and the cap is closed to the opening of the syringe in response to the strong centrifugal force, and the force must rely on the precise configuration design to derive the production cost. Improve the shortcomings. Moreover, in order not to interfere with the effect of stratification of the centrifuged blood, the device still has the influence of gravity as described above, so that it is difficult to disturb the stratified blood during operation, erroneously discharge PRP, and reduce the extraction effect. Effective recovery of the buffy coat.

In summary, the current techniques for separating PRP still have operational inconveniences, such as improper operation, and are more likely to cause serious problems such as sample contamination. In order to solve the above problems and deficiencies, it is an object of skill in the art to provide an apparatus that is easy to operate and that facilitates efficient acquisition of PRP in a simplified manner.

In view of the above-mentioned lack of traditional technology and the need, one of the aims of the present invention is to provide a blood separation device with simplified structure, convenient operation and high precision, which can easily perform blood extraction, centrifugation and extraction of specific blood stratification by the same device. In addition to simplifying complicated procedures, it also reduces the risk of contamination of blood samples and improves the safety of operators.

In addition, another object of the present invention is to provide a blood separation device which significantly improves the separation procedure for separating platelet-rich plasma and improves the efficiency and precision of its separation.

According to the above object, the present invention provides a blood separation device comprising: a sleeve having a first end and a second end in an axial direction, the first end forming a first opening and the second end forming a second end a second opening; an inner rod assembly is coaxially disposed in the sleeve in a sleeve manner to reciprocate the inner rod assembly in the axial direction in the sleeve; wherein the inner rod assembly further comprises a rod body and a rod The tank body, the rod body and the groove system are screwed so that the rod body can be detached from the tank body after being connected to the tank body; a top cover is disposed coaxially with the sleeve at the first end to be sleeved in the first opening, The bottom cover is closed, and a bottom cover is disposed coaxially with the sleeve at the second end to be sleeved to the second opening, thereby closing the second opening; wherein the bottom cover further has a central through hole for the rod body to The axial direction passes through the central through hole and the second opening and is connected to the groove body.

According to the blood separation device described above, the sleeve is tapered at a proximal end to form a body, and the body portion is further connected to a needle or an infusion line.

According to the foregoing blood separation device, wherein the bottom cover is sleeved to the second end by a screw connection;

According to the blood separation device described above, the peripheral edge of the center through hole is in contact with the rod body, so that the center through hole supports the rod body.

According to the blood separation device described above, the sleeve has a sleeve inner wall and an outer wall; the groove body has a groove side inner wall, a groove side outer wall and a groove bottom; the groove side outer wall is in contact with the inner wall, thereby making the groove The body sealing sleeve; the inner wall of the groove side and the bottom of the groove together form an accommodating space.

According to the blood separation device described above, the material of the tank body and the sleeve is selected from the group consisting of polyethylene, polypropylene, polycarbonate, silicone, polyurethane, polymethyl methacrylate, and a mixture of the foregoing materials.

10‧‧‧ Inner rod assembly
11‧‧‧ rod body
13‧‧‧
20‧‧‧Top cover
30‧‧‧ sleeve
40‧‧‧ bottom cover
100‧‧‧ Blood separation device
110‧‧‧First threaded part
112‧‧‧Handheld Department
114‧‧‧Support
116‧‧‧blocking parts
130‧‧‧ accommodating space
132‧‧‧ side wall
134‧‧‧Second thread
136‧‧‧ bottom of the trough
210‧‧‧拴塞部
220‧‧‧First Guide
240‧‧‧Second guidance
260‧‧‧ guiding bevel
320‧‧‧ body
340‧‧‧Sleeve wall
360‧‧‧ Third thread
410‧‧‧Center through hole
420‧‧‧Four threaded part
1120‧‧‧Pressing parts
1140‧‧‧ edges
1320‧‧‧ ribbed notch
1322‧‧‧Slot side inner wall
1324‧‧‧Slot side outer wall
1326‧‧‧First seal ring
3001‧‧‧ first end
3002‧‧‧ second end
3100‧‧‧ first opening
3200‧‧‧ second opening
3402‧‧‧ sleeve inner wall
3404‧‧‧Sleeve outer wall
4102‧‧‧ Track
1320a‧‧‧Smooth notch
1320b‧‧‧ inner chute
1320c‧‧‧ concave notch
4102a‧‧ Track
4102b‧‧‧ Track
4102c‧‧‧ Track
4104c‧‧‧Second seal ring
410a‧‧‧Center through hole
410b‧‧‧ center through hole
410c‧‧‧ center through hole
501‧‧‧ needle
502‧‧‧Infusion line
B‧‧‧ blood
C‧‧‧ center axis
L1‧‧‧ platelet plasma layer
L2‧‧‧ platelet rich plasma layer
L3‧‧‧Blood brown layer
L4‧‧‧ red blood cell layer
S1, S2, S3, S4‧‧‧ steps
X‧‧‧ radial direction
Y‧‧‧ axial direction
Z‧‧‧Gravity direction

Figure 1 is a perspective view of a blood separation device according to the present invention.
Figure 2 is a perspective view of a blood separation device according to the present invention.
Figure 3 is a schematic exploded view of the blood separation device according to the present invention.
Figure 4 is a schematic cross-sectional view of a blood separation device according to the present invention.
Figure 5 is a schematic cross-sectional view of a top cover in accordance with one embodiment of the blood separation device of the present invention.
Figure 6A is a schematic cross-sectional view of a trough body according to a first embodiment of the blood separation device of the present invention.
Figure 6B is a schematic cross-sectional view of a trough body according to a second embodiment of the blood separation device of the present invention.
Figure 6C is a schematic cross-sectional view of a trough body according to a third embodiment of the blood separation device of the present invention.
Figure 6D is a schematic cross-sectional view of a trough body according to a fourth embodiment of the blood separation device of the present invention.
Fig. 7A is a schematic cross-sectional view showing a tank body according to a fifth embodiment of the blood separating apparatus of the present invention.
Fig. 7B is a schematic cross-sectional view showing a tank body according to a sixth embodiment of the blood separating apparatus of the present invention.
Fig. 7C is a schematic cross-sectional view showing a tank body according to a seventh embodiment of the blood separating apparatus of the present invention.
Figure 7D is a schematic cross-sectional view of a trough body according to an eighth embodiment of the blood separation device of the present invention.
Figure 8 is a schematic flow diagram of a method for separating platelet-rich plasma according to the blood separation device of the present invention.

In order to make the purpose, technical features and advantages of the present invention more understandable to those skilled in the relevant technical field, and to implement the present invention, the technical features and implementation manners of the present creation are specifically explained in conjunction with the drawings, and The preferred embodiment is further illustrated. The drawings in the following texts are intended to be illustrative of the features of the present invention and are not required to be completely drawn according to the actual situation; and the description of the present embodiment relates to the technical content well known to those skilled in the art. I will not repeat them here.

First of all, the blood separation device of this creation is mainly used to separate the buffy coat in platelet-rich plasma or platelet-rich plasma. However, based on the common knowledge in the art, and at the same time according to the listed figures and the following description, the application of the present invention is not limited to platelet-rich plasma, and it is reasonable to push various blood components which can be stratified by centrifugation. Or organize the specimen, and for the sake of brief explanation, the following is referred to as "blood separation device".

Referring to Figures 1 and 2, a perspective view of a blood separation device 100 according to the present invention, and a third embodiment, is an exploded view of the blood separation device 100 according to the present invention. Since the blood separation device 100 of the present invention has a substantially elongated cylindrical shape in appearance, the entire blood separation device 100 has an imaginary central axis C. In order to facilitate the description of the present invention, the spatial direction of the blood separation device 100 is first defined according to the direction of the central axis C, and is defined as the "axial direction" Y along the longitudinal direction of the central axis C; and perpendicular to the central axis C. The cross-sectional direction is defined as "radial direction" X. The "coaxial setting" described in the following description of each component setting method refers to the central axis symmetry point of each component as an axis, and is sequentially arranged along the axial direction of the central axis C, which can be used by those skilled in the art. It is easy to understand the means of setting up, so it will not be detailed.

The blood separation device 100 according to the present invention comprises a sleeve 30, an inner rod assembly 10, a top cover 20, and a bottom cover 40. The sleeve 30 has a first end 3001 and a second end 3002 (see FIG. 3) along the axial direction Y. The first end 3001 forms a first opening 3100, and the second end 3002 forms a second opening. 3200. The first opening 3100 is an opening for the blood to enter and exit the blood separation device 100. After the blood separation device 100 is assembled, the second opening 3200 is maintained in a sealed state by other members, as will be described later.

The sleeve 30 is substantially hollow cylindrical shape, and is close to the first end 3001. The diameter of the cylinder is gradually reduced to a body portion 320, so that the seat portion 320 can be further connected with a needle or other infusion line for self-patients. The body draws blood into the sleeve 30, or the sample contained within the sleeve 30 (e.g., stratified blood) is output by a needle or other infusion line for subsequent medical use. Specifically, the blood separation device of the present invention can be applied to commercially available needles, butterfly needles, infusion sets, or other medical devices that can be coupled to the body portion 320. In addition, the creation of the sleeve 30 is not particularly limited, but for the purpose of blood separation, observing the stratification of the blood helps to achieve a better separation effect, so the material of the sleeve 30 needs to have an ideal transparency. Therefore, the operator can easily observe and distinguish the state of the blood sample in the sleeve, such as the volume, the liquid level and the blood stratification position. Therefore, the material of the sleeve 30 of the present invention is selected from the group consisting of polyethylene and high density poly. A group consisting of ethylene, polypropylene, polycarbonate, silicone, polyurethane, polymethyl methacrylate, and a mixture of the foregoing materials is a preferred embodiment.

The inner rod assembly 10 is coaxially disposed in the sleeve 30 in a sleeved manner. However, the inner rod assembly 10 and the sleeve 30 are not fixed, so that the inner rod assembly 10 can be axially oriented in the sleeve 30. Reciprocating motion; in other words, the inner rod assembly 10 is sleeved in a hollow duct in the sleeve 30, and can be moved in the axial direction Y toward the first end 3001 or the second end 3002 by applying an external force. motion. In addition, the inner rod assembly 10 further includes a rod body 11 and a groove body 13. The rod body 11 and the groove body 13 are screwed so that the rod body 11 can be detached from the tank body 13 after being connected to the tank body 13. That is, the inner rod assembly 10 is composed of the rod body 11 and the trough body 13, and the two are firmly connected integrally by a screw connection, that is, the inner rod assembly 10, wherein the rod body 11 serves as a force applying portion for reciprocating motion. The tank body 13 has the function of a piston, which can push the blood sample in the sleeve 30; in addition, the tank body 13 also functions to close the second opening 3200, achieving a liquid sealing effect, and separating the blood in use. The blood sample of the device 100 is sealed in the sleeve 30, does not leak from the second opening 3200, and does not leak even if subjected to a strong external force during centrifugation.

According to a preferred embodiment of the present invention, the rod body 11 and the groove body 13 are preferably threadedly connected, because the threaded connection is a widely used detachable fixed connection means, and the related manufacturing technology is mature and easy to select, and has a simple structure. The characteristics of the stable connection are beneficial to strengthening the structural strength of the inner rod assembly 10 as a whole, and corresponding to the stability of the inner rod assembly 10 when moving in the sleeve 30, and reducing the disturbance; in addition, during the separation process, there is a need for disassembly Because of the easy-to-disassemble nature of the threaded connection, the disassembly action does not easily affect the stability of the whole blood separation device 100, especially for the stratified blood, which is not easy to cause disturbance, and thus is beneficial to the overall effect of blood separation.

On the other hand, please refer to Figures 3 and 4; Figure 4 is a schematic cross-sectional view of the blood separation device 100 according to the present invention. The sleeve 30 has a sleeve wall 340. The sleeve wall 340 further has a sleeve inner wall 3402 and a sleeve outer wall 3404. The side wall 132 of the groove body 13 has a groove side inner wall 1322 and a groove side outer wall 1324. a groove bottom 136; the groove side outer wall 1324 is in contact with the sleeve inner wall 3402 to sufficiently generate a frictional force, so that the groove body 13 seals the sleeve 30, that is, the sleeve 30 is near the second end 3002 by the groove The setting of the body 13 is in a state of being closed.

However, it should be noted that even if the groove body 13 achieves the sealing effect of the sleeve 30, the groove body 13 can be reciprocally moved in the sleeve 30 by external force. In addition, the groove side inner wall 1322 and the groove bottom portion 136 together form an accommodating space 130. On the one hand, the accommodating space 130 can expand the space of the blood sample body of the sleeve 30, thereby increasing the utilization efficiency of the accommodating space 130; In the aspect, a sterile anticoagulant can be pre-placed therein, and the manufacturer can prescribe the anticoagulant in the accommodating space 130 according to the volume of the predetermined blood sample, and then deliver it directly to the user. The blood drawing operation saves the user the procedure of preparing and measuring the anticoagulant before taking blood, and reduces the risk of operational contamination. The type of the anticoagulant is not limited, but it is common knowledge in the art that the type of anticoagulant has different choices depending on the purpose of blood separation. For example, trisodium citrate is commonly used. Extract the anticoagulant used in PRP.

As can be seen from the above description, the tank body 13 has the effect of sealing the sleeve 30 in the blood separation device 100 (similar to the function of a piston provided by a general syringe), the function of pushing the specimen, and pre-accommodating the anticoagulant. The function of the material is selected from the principle of having sufficient elasticity and mechanical strength. Therefore, the material of the groove 13 is selected from the group consisting of polyethylene, polypropylene, polycarbonate, silicone, and polyurethane. A group of polymethyl methacrylate and a mixture of the foregoing materials is a preferred embodiment. According to a preferred embodiment of the present invention, high density polyethylene (High Density Polyethylene (HDPE) is more desirable, and has the following advantages: HDPE material is a thermoplastic plastic material, easy to process and suitable for manufacturing various injection and blow molding. And extrusion molding products, which are easy to manufacture and have low impact on the environment; in terms of mechanical properties, HDPE is tough, has good elasticity and softness; in addition, HDPE has high biocompatibility, abrasion resistance and lubrication. It is very suitable for the blood separation device 100 of the present invention to achieve the various effects exerted by the above-mentioned tank body 13.

According to the function of the above-mentioned creation tank 13, the configuration can be adjusted according to the needs of the user. The first to fourth embodiments are listed below, and the 6A to 6D drawings are used to more fully explain the groove 13 of the present invention. Embodiments, but the tank of the creation includes but is not limited to the following aspects.

First Embodiment: Referring to FIG. 6A, the groove side inner wall 1322 and the groove bottom 136 of the groove body 13 form an accommodation space 130, and a second thread portion 134 is formed in the axial direction Y by the groove bottom portion 136 for the rod body. 11 locks. The groove side outer wall 1324 of the groove body 13 protrudes outward at the open end, so that the open end of the groove body 13 forms a rib groove 1320, which can increase the frictional force between the groove side outer wall 1324 and the sleeve inner wall 3402. This design can improve the sealing of the groove 13 to the sleeve 30, and can also enhance the stability of the groove 13 when reciprocatingly sliding in the sleeve 30. In order to facilitate the succinct description of the embodiment of the present invention, the blood separation device of the present invention will be described below with reference to the aspect of the tank body 13 of the first embodiment.

Second Embodiment: Referring to FIG. 6B, the groove side outer wall 1324 of the groove body 13a is kept smooth at the open end, so that the open end of the groove body 13 forms a smooth notch 1320a, and the rest of the configuration is substantially the same as the foregoing first embodiment. Therefore, it will not be repeated. The configuration of the smooth notch 1320a is very simple to manufacture. Therefore, when the elasticity and roughness of the material of the selected groove body 13 are moderate, the desired friction force can be achieved and the functional requirements of the groove body are met, so the smooth groove is obtained. The 1320a is designed to reduce manufacturing costs.

Third Embodiment: Referring to FIG. 6C, the groove-side outer wall 1324 of the groove body 13b is inclined toward the accommodating space 130 at the open end, so that the open end of the groove body 13 forms an inner oblique groove 1320b, and the remaining configurations are substantially the same. In the foregoing first embodiment, it will not be described again. The configuration of the inner oblique slot 1320b is also a simple manufacturing aspect, and the inner oblique groove 1320b is designed to improve the stability of the movement of the blood sample when the movement is carried out after the blood is contained, so as to reduce the generation. The possibility of disturbance, especially after the blood sample is centrifugally layered, when the user pushes and pulls the inner rod assembly 10 for extraction of a specific blood layer, the aforementioned effect of reducing the disturbance can make the extraction more precise and help to improve the extraction effect.

Fourth Embodiment: Referring to FIG. 6D, the groove side outer wall 1324 of the groove body 13c forms a notch at the open end, so that the open end of the groove body 13 forms a concave notch 1320c, and the remaining configuration is substantially the same as the first The embodiment is not described again. In order to achieve the aforementioned effect of the groove body 13c, a first sealing ring 1326 is added to the concave groove 1320c so as to be adapted to be coupled to the concave groove 1320c for enhancing the friction force and the corresponding effect. (Sealed, stable sliding). When the material of the selected tank body 13c is a general plastic, the physical properties of the tank 13c can not be immediately generated, which is especially suitable for the design of the first sealing ring 1326 of the present embodiment, in response to the tank body 13c. Functional requirements.

The rod body 11 is screwed to the groove body 13 and has a detachable property. Referring to FIGS. 3 and 4, when the rod body 11 is disposed, the first thread portion 110, the support portion 114, the blocking member 116, and the hand portion 112 are sequentially formed from the first end 3001 toward the second end 3002 in the axial direction Y. For the first threaded portion 110, the groove body 13 forms a second threaded portion 134 corresponding to the first threaded portion 110, and the first threaded portion 110 and the second threaded portion 134 are screwed to each other, so that the rod body 11 and the groove body 13 are The lock can be achieved, and the two can be loosened by reverse operation to achieve the purpose of unloading, so that the rod body 11 can be easily removed from the tank body 13 and taken out of the sleeve 30 for further use. The locking principle of the threaded connection can be easily understood by those skilled in the art and will not be described again.

The support portion 114 supports the structural strength of the rod body 11, and after the rod body 11 is coupled to the tank body 13, the effect of supporting the integral inner rod assembly 10 can also be achieved. The present invention does not limit the type of the rod body 11, but it is required to provide the structural strength of the integral rod body 11 and the inner rod assembly 10, so that it can be constituted by a rod body of a simple configuration (for example, a cylinder, a hollow rod, a prism); or According to a preferred embodiment of the present invention, the plurality of edges 1140 (see FIGS. 1, 2, and 3) may be used to form the support portion 114, so that the support portion 114 stably drives the inner rod assembly 10 when subjected to a force. The sleeve 30 is reciprocated and the arrangement of the lightweight blood separation device 100 is achieved while saving the manufacturing material.

The blocking member 116 is generally disc-shaped to limit the distance that the rod 11 is advanced toward the first end 3001. The blocking member 116 has a blocking effect when the inner rod assembly 10 reaches a predetermined advancement amplitude, so that the inner rod assembly 10 advances. It can conform to the length of the sleeve 30, avoiding excessive pushing of the inner rod assembly 10 and affecting the operation effect.

The hand-held portion 112 is used for the hand grip of the user during operation, and the user can apply the force to the hand-held portion 112 to achieve the effect of driving the inner rod assembly 11 to reciprocate. To facilitate operation, the hand-held portion 112 may further form a pressing member 1120 at its end, the configuration of which includes but does not occur on the fins, the discs, and the ribs, so that the user can easily apply the inner rod assembly 10. The specific function of the pressing member 1120 is to expand the area that the finger can be pressed when the inner rod assembly 11 is pushed, and to apply the biasing force to the finger when the inner rod assembly 11 is pulled back.

Referring to FIG. 5, the top cover 20 of one embodiment of the present invention is disposed coaxially with the sleeve 30 at the first end 3001 to be sleeved to the first opening 3100, thereby closing the first opening 3100. The top cover 20 is used to close the first opening 3100 to prevent the blood sample from being exposed or leaked when the blood separation device 10 is filled with the blood sample. Especially during centrifugation, the top cover 20 ensures that the blood sample has no external leakage. To achieve this effect, the present invention provides a capping 20 with a sealing effect according to the configuration of the first opening 3100 of the sleeve 30, which has a plug portion 210, a first guiding portion 220, a second guiding portion 240, and a guiding slope 260. The first guiding portion 220 and the second guiding portion 240 are juxtaposed to guide the first opening 3100 to travel inside the top cover 20 to achieve closure. Referring to FIG. 4, when the top cover 20 is installed, the seat portion 320 of the sleeve 30 can enter the top cover 20 along the guiding slope 260, and is guided by the first guiding portion 220 and the second guiding portion 240. The plug portion 210 is inserted into the first opening 3100, and after being inserted into the plug portion 210, the first guiding portion 220 further produces the effect of holding the first end 3001, increasing the strength of the external force, and enhancing the sealing effect.

Referring to Figures 3 and 4, in accordance with an embodiment of the present invention, the bottom cover 40 is disposed coaxially with the sleeve 30 at the second end 3002 to fit over the second opening 3200, thereby closing the second opening 3200. The socket of the bottom cover 40 and the sleeve 30 is screwed to the fourth threaded portion 420 of the bottom cover 40 by the third threaded portion 360 formed by the sleeve 30 at the second end 3002 to achieve the locking effect. . The bottom cover 40 further has a central through hole 410 for connecting the rod body 11 in the axial direction Y through the center through hole 410 and the second opening 3200 to the groove body 13. The central through hole 410 of the bottom cover 40 further forms a rail 4102 inwardly (see Fig. 7A) for guiding and holding the rod body 11.

More specifically, in order to enhance the blood separation effect, when the blood is subjected to centrifugal stratification, it is necessary to avoid disturbing the sample as much as possible in operation. The rod body 11 is substantially elongated. When the force is reciprocating, there may be instability and disturbing of the specimen. Therefore, by providing the rail 4102 to support the rod body 11, a stable integral inner rod is achieved. The effect of the assembly 10 and correspondingly enhances the separation quality. In other words, when the inner rod assembly 10 reciprocates in the axial direction Y in the sleeve 30, the central through hole 410 and the rail 4102 continuously support the rod body 11 to achieve the effects of positioning, stabilization, and guiding.

According to the function of the bottom cover 40, the material of the bottom cover 40 is not particularly limited, and includes, but not limited to, polyethylene, polypropylene, polycarbonate, silicone, polyurethane, polymethyl methacrylate, and the foregoing materials. mixture. In addition, in response to the demand for hand-held operation, a three-dimensional texture can be selectively formed on the outer wall of the bottom cover 40 to increase the friction force to achieve a labor-saving grip.

On the other hand, the bottom cover 40 also strengthens the structural strength of the blood separation device 100, so that the blood separation device 100 can be directly applied to centrifugation. Therefore, the bottom cover 40 is supplemented by the other components described above, so that the blood separation device 100 of the present invention can perform blood collection, centrifugation, and extraction in the same device, thereby simplifying the operation process and eliminating the cumbersome process required for replacing the container or pipetting. It also reduces the risk of contamination that may arise from complex processes. In addition, the blood separation device 100 according to the present invention simultaneously overcomes the directional limitation of the conventional blood separation technique based on a conventional syringe (the blood inlet and outlet of the syringe need to be placed downward to be centrifuged, so as to limit the operation direction when extracting a specific blood layer). And its derivatives are missing.

According to the function of the bottom cover of the present invention, the configuration can be adjusted according to the needs of the user. The following fifth to eighth embodiments are listed, together with the figures 7A to 7D, to fully explain the implementation of the bottom cover of the present invention. Way, but the bottom cover of this creation includes but is not limited to the following aspects.

Fifth Embodiment: Referring to FIG. 7A, the basic structure of the bottom cover 40 has been previously described, that is, the center opening 410 of the trough body extends into the cover body and the inner diameter is reduced to form a rail 4102, so that the inner diameter of the rail 4102 is approximately the same. The outer diameter of the support portion 114 of the rod body 11 is matched so that the rail 4102 can support the rod body 11. In use, during the operation of the blood separation device 100 of the present invention, there is a need to remove and install the rod body 11. Therefore, the inner diameter of the center port 410 is larger than the inner diameter of the rail 4102 to allow the rod body 11 to be easily centered. The port 410 enters or exits the sleeve 30, and the design of the track 4102 simultaneously achieves guiding and retaining the effect of the rod 11. The center port 410 and the track 4102 taken in this embodiment have an inverted T shape in cross-section appearance, and are easy to manufacture. In order to facilitate the simplification of the implementation of the present invention, this description is described in terms of the bottom cover 40.

Sixth Embodiment: Referring to Figure 7B, the inner diameter of the central through hole 410a of the bottom cover 40a is substantially equal to the inner diameter of the rail 4102a, providing a more easily manufactured bottom cover pattern, and the remaining configurations are substantially the same as the foregoing The fifth embodiment will not be described again.

Seventh Embodiment: Referring to FIG. 7C, the inner diameter of the central through hole 410b of the bottom cover 40b is larger than the inner diameter of the rail 4102b, and the edge of the central through hole 410b presents an inwardly concave slope so that the central opening 410b can be lifted. The effect of the rod body 11 is that after the rod body 11 enters the center port 410b, the rails 11 are guided by the inclined surface to enter or exit the rail 4102b, so that the rod body 11 can more easily enter or leave the sleeve 30 from the center port 410. The rest of the configuration is substantially the same as the foregoing fifth embodiment and will not be described again.

Eighth embodiment: Referring to FIG. 7D, the central through hole 410c of the bottom cover 40c and the rail 4102c are substantially identical in configuration to the fifth embodiment, and a second sealing ring 4104c may be added according to the use. The inner diameter of the central through hole 410c is adapted to be in contact with the rod body 11 at the periphery of the central through hole 410c for reinforcing the frictional force of the central through hole 410c with respect to the rod body 11 and the corresponding effect, so as to correspond to the bottom cover 40c. Functional requirements.

In view of the above-described blood separation device according to the present invention, the present invention further provides a simplified and reliable method for isolating platelet-rich plasma based on the blood separation device described above. Since the components, composition and functional effects of the blood separation device of the present invention have been described above, they will not be described in detail. However, the operation includes, but is not limited to, two main aspects: when the blood separation device is assembled, the tank body is pre-placed with an anticoagulant; and the other is used by the user to take anticoagulation before use. Blood agent.

In the following, when the blood separation device is assembled, the tank body is preliminarily placed with an anticoagulant, and the method of separating the platelet-rich plasma includes the following steps:

Step S1: the top cover 20 is removed, and a needle 501 is mounted on the first end of the sleeve 30. The blood B of the patient is extracted by using the blood separation device equipped with the needle 501, so that the blood B enters the sleeve 30. That is, thoroughly mixed with the anticoagulant; then the needle 501 is removed, and the top cover 20 is sleeved to the first opening to close the first opening;

Step S2: The rod body 11 is removed, and the rod body 11 is removed from the tank body 13, so that the rod body 11 is disengaged from the center through hole of the bottom cover 40 and the top cover 20 is installed to seal the first opening.

Step S3: centrifuging the blood separation device with the second end toward the gravity direction Z to stratify the blood B. The blood B after the centrifugal layering has a plurality of layers, and the first end includes a small amount in the direction from the first end to the second end. Platelet plasma layer L1, platelet rich plasma layer L2, buffy coat layer L3, red blood cell layer L4.

Step S4: the rod body 11 is inserted into the 30 sleeve from the center through hole, and is screwed to the tank body 13 to be restored into a functional inner rod assembly; then, the push rod body 11 drives the inner rod assembly to The sleeve 30 reciprocates to drive the layer of blood to move in the sleeve to adjust the layered position, so that the layers are sequentially discharged from the first opening, and the user can directly discharge the layers. Aspirate the target layer (platelet plasma layer L2, buffy coat layer L3), or install a needle, an infusion line 502 or a suitable container at the first end of the sleeve 30 to receive the stratified blood layer to extract the layer The platelet-rich plasma layer L2, the buffy coat layer L3 or both are extracted simultaneously. According to a preferred embodiment of the present invention, the butterfly needle infusion line is directly used as the infusion line 502, so that the target layered blood can be directly injected back into the affected part of the patient. Not only easy to operate, but also reduce the risk of pollution. In addition, according to another embodiment of the present invention, the user may use an adapter having a bidirectional through hole, one end of which is attached to the sleeve 30, and the other end is connected to an appropriate infusion line or Appropriate containers (eg, sterile empty syringes) are used to securely engage the two and simultaneously provide a blood circulation conduit to stratify specific blood (eg, platelet plasma layer L2 or buffy coat L3) from the sleeve 30 is delivered to infusion line 502 or a suitable container.

According to another aspect, the user takes the anticoagulant before use, and the operation method is substantially the same as the above step, but the user must measure the appropriate anticoagulant before blood collection.

However, the above description is only a preferred embodiment of the present invention, and thus does not limit the scope of the patent of the present invention. Therefore, all the simple modifications and equivalent structural changes using the present specification and the schema should be the same. Included in the scope of this creation patent.

100‧‧‧ Blood separation device

10‧‧‧ Inner rod assembly

11‧‧‧ rod body

13‧‧‧

30‧‧‧ sleeve

40‧‧‧ bottom cover

130‧‧‧ accommodating space

320‧‧‧ body

X‧‧‧ radial direction

Y‧‧‧ axial direction

Z‧‧‧Gravity direction

C‧‧‧ center axis

Claims (7)

A blood separation device for separating platelet rich plasma, comprising:
a sleeve having a first end and a second end in an axial direction, the first end forming a first opening, the second end forming a second opening;
An inner rod assembly is coaxially disposed in the sleeve in a sleeve manner to reciprocate the inner rod assembly in the axial direction in the sleeve; wherein the inner rod assembly further comprises a rod body and a rod a groove body, the rod body is screwed to the groove system, so that the rod body can be arbitrarily removed from the groove body after being connected to the groove body;
a cover disposed coaxially with the sleeve at the first end to be sleeved in the first opening to close the first opening; and a bottom cover disposed coaxially with the sleeve at the second end The second opening is closed to cover the second opening; wherein the bottom cover further has a central through hole, the rod body passes through the central through hole and the second opening and the groove body along the axial direction connection. The blood separation device according to claim 1, wherein the sleeve is tapered toward the first end to form a body, and the body portion is further connected to a needle or an infusion line. The blood separation device of claim 1, wherein the bottom cover is threaded to the second end. The blood separation device according to claim 1, wherein the peripheral edge of the central through hole is in contact with the rod body such that the central through hole supports the rod body. The blood separation device according to claim 1, wherein the sleeve has a sleeve inner wall and a sleeve outer wall; the groove body has a groove side inner wall, a groove side outer wall and a groove bottom; The groove side outer wall is in contact with the inner wall of the sleeve, so that the groove body seals the sleeve; the groove side inner wall and the groove bottom together form an accommodation space. The blood separation device according to claim 1, wherein the material of the tank is selected from the group consisting of polyethylene, polypropylene, polycarbonate, silicone, polyurethane, polymethyl methacrylate, and a mixture of the foregoing materials. Group. The blood separation device according to claim 1, wherein the material of the sleeve is selected from the group consisting of polyethylene, high density polyethylene, polypropylene, polycarbonate, silicone, polyurethane, polymethyl methacrylate, and the foregoing A group of material mixtures.