CN103405818B - Prepare the devices and methods therefor of Platelet-rich plasm - Google Patents
Prepare the devices and methods therefor of Platelet-rich plasm Download PDFInfo
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- CN103405818B CN103405818B CN201310385474.8A CN201310385474A CN103405818B CN 103405818 B CN103405818 B CN 103405818B CN 201310385474 A CN201310385474 A CN 201310385474A CN 103405818 B CN103405818 B CN 103405818B
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Abstract
nullThe present invention relates to the devices and methods therefor preparing Platelet-rich plasm，This device includes: piston、Recessed mistress manages、Convex mistress manages、Piston bush、Interior room is managed、Push-pull bar、Syringe needle and enclosing cover，Convex mistress's pipe is built with piston bush，Piston bush moves along convex mistress's inside pipe wall，Manage built with interior room at piston bush，Piston bush is managed by threadeding with interior room，At interior room pipe built with push-pull bar，Push-pull bar moves along interior room inside pipe wall,The inner chamber of convex mistress's pipe is led in the one end having this centre bore of centre bore at piston bush center，The other end leads to the inner chamber of interior room pipe，The front end of convex mistress's pipe is a boss outwardly，Central authorities at this boss have a pin hole，Syringe needle it is inserted with in pin hole，Or on boss, it is cased with enclosing cover，Or manage equipped with recessed mistress at boss，At recessed mistress's pipe built with piston，This piston moves along recessed mistress's inside pipe wall，The front center place of recessed mistress's pipe has blood receiving hole，One end of blood receiving hole communicates with recessed mistress's pipe，The other end communicates with pin hole.
Description
Technical field
The present invention relates to a kind of device of mixed layer isolating plasma layer and platelet and blood plasma from blood and preparation method thereof.
Background technology
Injured or postoperative each recovery process is the challenge to physical ability regeneration.Platelet plays an important role in regenerative process.Platelet can promote the solidification of blood and the healing of wound, also has important effect in tissue regeneration simultaneously, because they can produce messenger substances, and namely so-called somatomedin.These somatomedin absorb stem cell and reserve cell, and induce differentiation.By rising in value, new organization is the formation of.
In stem cell and reserve cell, somatomedin act as " Magnet ".If the ability of " Magnet " is more strong, then be absorbed by more stem cell and reserve cell, thus reducing healing time.
As can be seen here, from blood, isolate platelet most important, but it is complicated to isolate hematoblastic device at present from blood, and to add activator (such as solidifyingization enzyme) in blood and activate or the hematoblastic release of catalysis.
Summary of the invention
It is an object of the invention to overcome the defect of prior art, and provide the simple syringe of a kind of use and the centrifuge just can the device that isolate plasma layer and platelet and blood plasma mixed layer from blood and preparation method thereof.
In order to complete the purpose of the present invention, the present invention by the following technical solutions:
nullA kind of device preparing Platelet-rich plasm of the present invention，It includes: piston、Recessed mistress manages、Convex mistress manages、Piston bush、Interior room is managed、Push-pull bar、Syringe needle and enclosing cover，Wherein: convex mistress's pipe is built with piston bush，Piston bush moves along convex mistress's inside pipe wall，Manage built with interior room at piston bush，Piston bush is managed by threadeding with interior room，At interior room pipe built with push-pull bar，Push-pull bar moves along interior room inside pipe wall,The inner chamber of convex mistress's pipe is led in the one end having this centre bore of centre bore at piston bush center，The other end leads to the inner chamber of interior room pipe，The front end of convex mistress's pipe is a boss outwardly，Central authorities at this boss have a pin hole，Syringe needle it is inserted with in pin hole，Or on boss, it is cased with enclosing cover，Or manage equipped with recessed mistress at boss，At recessed mistress's pipe built with piston，This piston moves along recessed mistress's inside pipe wall，The front center place of recessed mistress's pipe has blood receiving hole，One end of blood receiving hole communicates with recessed mistress's pipe，The other end communicates with pin hole；
The device preparing Platelet-rich plasm of the present invention, wherein: the front of described convex mistress's pipe is cone outwardly, recessed mistress's pipe front is cambered inwards cone, and recessed mistress pipe intercouples with convex mistress's pipe, and the shape of the front end of piston bush is identical with the inner chamber front end geometry of convex mistress's pipe；
The device preparing Platelet-rich plasm of the present invention, wherein: described piston bush is made up of sleeve pipe and nose cone, nose cone, in the front end of sleeve pipe, has centre bore in cone headed central authorities, and interior room pipe is contained in sleeve pipe, and the front end of interior room pipe is connected with nose cone by screw thread；
The device preparing Platelet-rich plasm of the present invention, wherein: described sleeve pipe and nose cone are connected as a single entity；
The device preparing Platelet-rich plasm of the present invention, wherein: described enclosing cover passes through thread set on boss；
The device preparing Platelet-rich plasm of the present invention, wherein: described recessed mistress's pipe by thread set on boss；
The device preparing Platelet-rich plasm of the present invention, wherein: the volume of described recessed mistress's pipe is 50ml to 200ml.
The method preparing Platelet-rich plasm by assembly of the invention, comprises the following steps:
(A) being contained in by piston bush in convex mistress manages, interior room pipe is contained in piston bush, and push-pull bar is contained in the pipe of interior room, and syringe needle is inserted on the pin hole of convex mistress's pipe, pulls out piston bush and interior room pipe, is flowed into by blood sampling in the inner chamber of convex mistress's pipe；
(B) syringe needle is extracted from convex mistress's pipe, the boss of convex mistress's pipe covers enclosing cover, then shaken up, enclosing cover is straight down, convex mistress's pipe, piston bush, interior room pipe are put into the first time centrifugally operated carried out in centrifuge 5 minutes to 10 minutes together with push-pull bar, and the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min；
(C) said apparatus is vertically taken out from centrifuge, blood is divided into mixed layer and the plasma layer of hemocyte layer, platelet and hemocyte from bottom to up in convex mistress's tube cavity, enclosing cover is pulled out, boss accesses recessed mistress pipe and piston, piston is moved down along recessed mistress's pipe, after the recessed mistress's pipe of hemocyte layer suction undermost in convex mistress's tube cavity, being extracted by recessed mistress's pipe from convex mistress's pipe, hemocyte layer is stored in the inner chamber of recessed mistress's pipe；
(D) enclosing cover is covered again on the boss of convex mistress's pipe, then shaken up, enclosing cover is straight down, convex mistress's pipe, piston bush, interior room pipe are put into the second time centrifugally operated carried out in centrifuge 5 minutes to 10 minutes together with push-pull bar, and the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min；
(E) said apparatus is vertically taken out from centrifuge, blood is divided into mixed layer and the plasma layer of platelet and hemocyte from bottom to up in the inner chamber of convex mistress's pipe, push-pull bar is moved up along interior room pipe, after room pipe in the plasma layer suction of the superiors in convex mistress's tube cavity, interior room pipe is extracted from convex mistress's pipe；Plasma layer is stored in the tube cavity of interior room；The mixed layer of platelet and hemocyte is stored in recessed mistress's tube cavity, it is achieved that platelet and the mixed layer of hemocyte and separating of plasma layer.
The method of the present invention, wherein: the rotating speed of described centrifuge is 1500 revs/min.
The Platelet-rich plasm of the autologous conditions in this invention can directly operation, acquisition inside hospital operating room or office treatment.Additionally, double-chamber syringe (or first syringe is directly joined directly together) only need to be used with second syringe, without using substantial amounts of syringe, so just eliminating necessity of extra injection device and storage container, reduce the danger polluted.The main advantage obtaining the Platelet-rich plasm method of autologous conditions is exactly that double syringe can shift from bigger syringe to less syringe, without using several syringes.Additionally, the blood extracted activates or the hematoblastic release reaction of catalysis without any activator (such as solidifyingization enzyme), namely before centrifugal, among without add any can from the activator of intra platelet free calcium material or material.The risk polluted is greatly reduced, and the complexity of process also weakens.
Accompanying drawing explanation
Fig. 1 is under blood sampling state, the device preparing Platelet-rich plasm of the present invention；
Fig. 2 is under first time apocarpy and second time are centrifugal, the device preparing Platelet-rich plasm of the present invention；
Fig. 3 is after first time is centrifugal, extracts the original state of the device preparing Platelet-rich plasm of the present invention of hemocyte；
Fig. 4 is after first time is centrifugal, extracts the end-state of the device preparing Platelet-rich plasm of the present invention of hemocyte；
Fig. 5 is after second time is centrifugal, extracts the device preparing Platelet-rich plasm of the present invention of blood plasma.
In Fig. 1 to Fig. 5, label 1 is piston；Label 2 is recessed mistress pipe；Label 3 is enclosing cover；Label 4 is convex mistress pipe；Label 5 is piston bush；Label 6 is interior room pipe；Label 7 is push-pull bar；Label 8 is syringe needle；Hole centered by label 9；Label 10 is sleeve pipe；Label 11 is nose cone；Label 12 is pin hole；Label 13 is boss；Label 14 is blood receiving hole.
Detailed description of the invention
As shown in Figures 1 to 5, a kind of device preparing Platelet-rich plasm of the present invention includes: piston 1, recessed mistress's pipe 2, convex mistress's pipe 4, piston bush 5, interior room pipe 6, push-pull bar 7, syringe needle 8 and enclosing cover 3, the volume of recessed mistress's pipe 2 is 50ml to 200ml.
nullConvex mistress's pipe 4 is built with piston bush 5，Piston bush 5 moves along convex mistress's pipe 4 inwall，At piston bush 5 built with interior room pipe 6，Piston bush 5 with interior room pipe 6 by threadeding，At interior room pipe 6 built with push-pull bar 7，Push-pull bar 7 moves along interior room pipe 6 inwall,A centre bore 9 is had at the center of piston bush 5，The inner chamber of convex mistress's pipe 4 is led in one end of this centre bore 9，The other end leads to the inner chamber of interior room pipe 6，The front end of convex mistress's pipe 4 is a boss 13 outwardly，Central authorities at this boss 13 have a pin hole 12，As shown in Figure 1，Syringe needle 8 it is inserted with in pin hole 12，Or as shown in Figure 2，Enclosing cover 3 passes through thread set on boss 13，Or as shown in Figure 3 and Figure 4，Recessed mistress's pipe 2 passes through thread set on boss 13，At recessed mistress's pipe 2 built with piston 1，This piston 1 moves along recessed mistress's pipe 2 inwall，The front center place of recessed mistress's pipe 2 has blood receiving hole 14，One end of blood receiving hole 14 communicates with recessed mistress's pipe 2，The other end communicates with pin hole 12.The front of convex mistress's pipe 4 is cone outwardly, and recessed mistress's pipe 2 front is cambered inwards cone, and recessed mistress's pipe 2 intercouples with convex mistress's pipe 4, and the shape of the front end of piston bush 5 is identical with the inner chamber front end geometry of convex mistress's pipe 4.Piston bush 5 is made up of sleeve pipe 10 and nose cone 11, and nose cone 11 is in the front end of sleeve pipe 10, and the central authorities at nose cone 11 have centre bore 9, and interior room pipe 6 is contained in sleeve pipe 10, and the front end of interior room pipe 6 is connected with nose cone 11 by screw thread.Sleeve pipe 10 and nose cone 11 are connected as a single entity.
The method preparing Platelet-rich plasm by apparatus of the present invention, comprises the following steps:
(A) as it is shown in figure 1, be contained in by piston bush 5 in convex mistress's pipe 4, interior room pipe 6 is contained in piston bush 5, push-pull bar 7 is contained in interior room pipe 6, syringe needle 8 is inserted on the pin hole 12 of convex mistress's pipe 4, pulls out piston bush 5 and interior room pipe 6, is flowed into by blood sampling in the inner chamber of convex mistress's pipe 4；
(B) as shown in Figure 2, syringe needle 8 is extracted from convex mistress's pipe 4, the boss 13 of convex mistress's pipe 4 covers enclosing cover 3, then shaken up, enclosing cover 3 is straight down, convex mistress's pipe 4, piston bush 5, interior room pipe 6 are put in centrifuge the first time centrifugally operated carried out 5 minutes to 10 minutes together with push-pull bar 7, and the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min, it is desirable to be 1500 revs/min；
(C) as shown in Figure 3 and Figure 4, said apparatus is vertically taken out from centrifuge, blood intracavity in convex mistress's pipe 4 is divided into mixed layer and the plasma layer of hemocyte layer, platelet and hemocyte from bottom to up, enclosing cover 3 is pulled out, boss 13 accesses recessed mistress's pipe 2 and piston 1, piston 1 is moved down along recessed mistress's pipe 2, after the recessed mistress's pipe 2 of hemocyte layer suction undermost in convex mistress's pipe 4 inner chamber, being extracted from convex mistress's pipe 4 by recessed mistress's pipe 2, hemocyte layer is stored in the inner chamber of recessed mistress's pipe 2；
(D) as shown in Figure 2, enclosing cover 3 is covered again on the boss 13 of convex mistress's pipe 4, then shaken up, enclosing cover 3 is straight down, convex mistress's pipe 4, piston bush 5, interior room pipe 6 are put in centrifuge the second time centrifugally operated carried out 5 minutes to 10 minutes together with push-pull bar 7, the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min, it is desirable to be 1500 revs/min；
(E) as shown in Figure 5, said apparatus is vertically taken out from centrifuge, blood is divided into mixed layer and the plasma layer of platelet and hemocyte from bottom to up in the inner chamber of convex mistress's pipe 4, push-pull bar 7 is moved up along interior room pipe 6, after room pipe 6 in the plasma layer suction of the superiors in convex mistress's pipe 4 inner chamber, interior room pipe 6 is extracted from convex mistress's pipe 4；Plasma layer is stored in pipe 6 inner chamber of interior room；The mixed layer of platelet and hemocyte is stored in recessed mistress's pipe 2 inner chamber, it is achieved that platelet and the mixed layer of hemocyte and separating of plasma layer.
Above description is explanation of the invention, is not the restriction to invention, and limited range of the present invention is referring to claim, and when without prejudice to the spirit of the present invention, the present invention can do the amendment of appropriate format.
Claims (7)
- null1. the device preparing Platelet-rich plasm，It includes: piston (1)、Recessed mistress manages (2)、Convex mistress manages (4)、Piston bush (5)、Interior room pipe (6)、Push-pull bar (7)、Syringe needle (8) and enclosing cover (3)，It is characterized in that: convex mistress pipe (4) is built with piston bush (5)，Piston bush (5) is managed (4) inwall along convex mistress and is moved，(6) are managed built with interior room at piston bush (5)，Piston bush (5) manages (6) by threadeding with interior room，(6) are managed built with push-pull bar (7) in interior room，Push-pull bar (7) is managed (6) inwall and is moved along interior room,A centre bore (9) is had at the center of piston bush (5)，The inner chamber of convex mistress pipe (4) is led in one end of this centre bore (9)，The other end leads to the inner chamber of interior room pipe (6)，The front end of convex mistress pipe (4) is a boss (13) outwardly，Central authorities at this boss (13) have a pin hole (12)，Syringe needle (8) it is inserted with in pin hole (12)，Or on boss (13), it is cased with enclosing cover (3)，Or on boss (13), manage (2) equipped with recessed mistress，(2) are managed built with piston (1) recessed mistress，This piston (1) is managed (2) inwall along recessed mistress and is moved，The front center place of recessed mistress pipe (2) has blood receiving hole (14)，One end of blood receiving hole (14) is managed (2) and is communicated with recessed mistress，The other end communicates with pin hole (12)，The front of convex mistress pipe (4) is cone outwardly，Recessed mistress pipe (2) front is cambered inwards cone，Recessed mistress pipe (2) intercouples with convex mistress pipe (4)，The inner chamber front end geometry that the shape of the front end of piston bush (5) manages (4) with convex mistress is identical，Piston bush (5) is made up of sleeve pipe (10) and nose cone (11)，Nose cone (11) is in the front end of sleeve pipe (10)，Central authorities at nose cone (11) have centre bore (9)，Interior room pipe (6) is contained in sleeve pipe (10)，The front end of interior room pipe (6) is connected with nose cone (11) by screw thread.
- 2. prepare the device of Platelet-rich plasm as claimed in claim 1, it is characterised in that: described sleeve pipe (10) and nose cone (11) are connected as a single entity.
- 3. prepare the device of Platelet-rich plasm as claimed in claim 2, it is characterised in that: described enclosing cover (3) by thread set on boss (13).
- 4. prepare the device of Platelet-rich plasm as claimed in claim 3, it is characterised in that: described recessed mistress pipe (2) by thread set on boss (13).
- 5. prepare the device of Platelet-rich plasm as claimed in claim 4, it is characterised in that: the volume of described recessed mistress pipe (2) is 50ml to 200ml.
- 6. the method preparing Platelet-rich plasm with claim 1 to 5 any device, comprises the following steps:(A) piston bush (5) is contained in convex mistress pipe (4), interior room pipe (6) is contained in piston bush (5), push-pull bar (7) is contained in interior room pipe (6), syringe needle (8) is inserted on the pin hole (12) of convex mistress pipe (4), pull out piston bush (5) and interior room pipe (6), flowed into by blood sampling in the inner chamber of convex mistress pipe (4)；(B) syringe needle (8) is extracted from convex mistress pipe (4), the boss (13) that convex mistress manages (4) covers enclosing cover (3), then shaken up, enclosing cover (3) is straight down, convex mistress managing (4), piston bush (5), interior room pipe (6) and push-pull bar (7) and puts into the first time centrifugally operated carried out in centrifuge 5 minutes to 10 minutes together, the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min；(C) said apparatus is vertically taken out from centrifuge, blood is managed intracavity in (4) convex mistress and is divided into hemocyte layer from bottom to up, the mixed layer of platelet and hemocyte and plasma layer, enclosing cover (3) is pulled out, at boss (13), the upper recessed mistress of access manages (2) and piston (1), along recessed mistress, piston (1) is managed (2) move down, after recessed mistress's pipe (2) of undermost hemocyte layer suction in convex mistress pipe (4) inner chamber, recessed mistress is managed (2) extract from convex mistress pipe (4), hemocyte layer is stored in the inner chamber of recessed mistress pipe (2)；(D) enclosing cover (3) is covered again on the boss (13) that convex mistress manages (4), then shaken up, enclosing cover (3) is straight down, convex mistress managing (4), piston bush (5), interior room pipe (6) and push-pull bar (7) and puts into the second time centrifugally operated carried out in centrifuge 5 minutes to 10 minutes together, the rotating speed of centrifuge is 1000 revs/min to 2000 revs/min；(E) said apparatus is vertically taken out from centrifuge, blood manages the mixed layer and plasma layer that are divided into platelet and hemocyte in the inner chamber of (4) from bottom to up convex mistress, push-pull bar (7) is managed (6) along interior room move up, behind room pipe (6) in the plasma layer suction of the superiors in convex mistress pipe (4) inner chamber, interior room pipe (6) is extracted from convex mistress pipe (4)；Plasma layer is stored in pipe (6) inner chamber of interior room；The mixed layer of platelet and hemocyte is stored in recessed mistress pipe (2) inner chamber, it is achieved that platelet and the mixed layer of hemocyte and separating of plasma layer.
- 7. method as claimed in claim 6, it is characterised in that: the rotating speed of described centrifuge is 1500 revs/min.
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| CN201310385474.8A CN103405818B (en) | 2013-08-28 | 2013-08-28 | Prepare the devices and methods therefor of Platelet-rich plasm |
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| CN201310385474.8A CN103405818B (en) | 2013-08-28 | 2013-08-28 | Prepare the devices and methods therefor of Platelet-rich plasm |
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| CN103405818B true CN103405818B (en) | 2016-07-06 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104198229B (en) * | 2014-09-03 | 2016-06-29 | 中国科学院植物研究所 | A kind of gas sampling syringe |
| CN105596014B (en) * | 2016-01-22 | 2018-05-25 | 黄鹭强 | A kind of double-jacket tube centrifugation injection device |
| CN108940613B (en) * | 2018-07-17 | 2020-01-17 | 深圳市北科融汇生命科技有限公司 | Blood sample separation centrifuge and separation method thereof |
| CN108904902A (en) * | 2018-09-14 | 2018-11-30 | 威海凯尔医疗科技有限公司 | Double syringe autologous plasma collection device and the method for collecting blood plasma |
| CN109332007B (en) * | 2018-10-16 | 2020-12-29 | 江苏省肿瘤医院 | Plasma separation device and plasma separation method |
| CN111974475B (en) * | 2020-08-20 | 2021-09-21 | 四川行之智汇知识产权运营有限公司 | Blood component separator |
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| CN201469752U (en) * | 2009-08-31 | 2010-05-19 | 金哲 | A device for extracting blood, separating and injecting serum, |
| CN102078644A (en) * | 2011-03-08 | 2011-06-01 | 吉林大学 | Simple, convenient and efficient device and method for extracting platelet-rich plasma |
| CN203458633U (en) * | 2013-08-28 | 2014-03-05 | 朱悦 | Device for preparing platelet rich plasma |
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| CN201469752U (en) * | 2009-08-31 | 2010-05-19 | 金哲 | A device for extracting blood, separating and injecting serum, |
| CN102078644A (en) * | 2011-03-08 | 2011-06-01 | 吉林大学 | Simple, convenient and efficient device and method for extracting platelet-rich plasma |
| CN203458633U (en) * | 2013-08-28 | 2014-03-05 | 朱悦 | Device for preparing platelet rich plasma |
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Effective date of registration: 20210113 Address after: 110000 Room 502, 18 Sanhao Street, Heping District, Shenyang City, Liaoning Province Patentee after: Shenyang kailitai Technology Co.,Ltd. Address before: No.42-1 Haikou street, Heping District, Shenyang, Liaoning 110000 Patentee before: Zhu Yue Patentee before: Ma Jinghong |
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