WO2013141436A1

WO2013141436A1 - Blood centrifugation container

Info

Publication number: WO2013141436A1
Application number: PCT/KR2012/002920
Authority: WO
Inventors: 맹성호; 김창용
Original assignee: (주)세원메디텍
Priority date: 2012-03-19
Filing date: 2012-04-17
Publication date: 2013-09-26
Also published as: KR101162750B1

Abstract

The present invention relates to a blood centrifugation container. The invention comprises: an upper case of which the upper end is sealed by means of a sealing cap and the lower end opens downwards via a link-through port; a lower case which is joined to the upper case and of which the lower end is open; a rising-and-falling cap which is fitted in a way that allows position adjustment in the lower case, and which adjusts the volume inside the lower case via position adjustment relative to the lower case; a first check valve which is fitted to the rising-and-falling cap and allows collected blood to pass through so as to be received in the lower case; an opening-and-closing part which opens or blocks the link-through port of the lower case; a second check valve for sucking out blood plasma held in the upper case; and a suction tube which is provided in the inner space of the upper case, is fitted at the upper end thereof to the second check valve, and extends downwards in the length direction. The blood centrifugation container of the present invention, which is configured as described above, allows blood to be injected and suctioned even without using a syringe needle and thus the invention can be used with greater peace of mind since the risk of infection is commensurately eliminated, and the pressure of the sealed inner space can be maintained at atmospheric pressure such that blood injection and suction takes place smoothly.

Description

Blood Centrifuge Vessel

The present invention relates to a container for blood centrifugation.

Plasma is a neutral, pale yellow liquid component from which blood cells are separated from blood, and contains water, platelets, growth factors, proteins, sugars, lipids, inorganic salts, and metabolites in addition to water. It keeps ions constant.

In particular, so-called platelet-rich plasma (hereinafter referred to as PRP) obtained by centrifugation of the plasma acts to reconstruct damaged parts of blood vessels, thereby concentrating it and improving skin wrinkles or skin or ligaments. Or for the purpose of strengthening and regenerating muscles. For example, arthritis, back pain, or injections into damaged areas such as shoulders or knee ligaments may be used to regenerate damaged areas.

In particular, since the PRP therapy uses its own blood, there are no side effects and the therapeutic effect is also fast. The blood centrifuge container for separating the PRP from the blood is used a lot.

1 is a cross-sectional view schematically showing a conventional blood centrifuge container.

As shown in the drawing, the conventional blood centrifuge container 11 has a cylindrical shape, the first storing part 13 having an upper end blocked by a closing cap 15 and an opening hole 21 formed at a lower end thereof. ), A second storage part 23 which is screwed to the lower end of the first storage part 13 and has a flow path opening and closing part 25 therein, and a water level for sealing the lower end of the second storage part 23. It consists of an adjusting unit (19).

A rubber member 17 is provided at the center of the sealing cap 15. The rubber member 17 is a rubber stopper for sealing the first storage part 13 and passes the injection needle C as shown in FIG. 2D. Conventional centrifuge container 11 can not insert the needle (C) without the rubber member (17).

In addition, the flow path opening and closing part 25 is supported by an inner circumferential surface of the second storage part 23 and provided with a cradle 25c for providing a flow path 25b therebetween, and a blocking plug 25a fixed to the cradle 25c. It is composed. The blocking plug 25a moves toward the opening hole 21 to block the opening hole 21 as the second housing part 23 moves toward the first storage part 13.

The water level control unit 19, the lifting cap (19a) for screwing the position adjustable to the second housing 23, and the inner side of the lifting cap (19a) of the second storage unit (23) It consists of a piston 19b inserted into the inside and a rubber member 17 located at the center of the piston 19b.

The piston 19b is moved up and down inside the second storage unit 23 by moving the lifting cap 19a up and down by turning the lifting cap 19a to adjust the internal volume of the second storage unit 23. do. In addition, the rubber member 17 serves to pass the injection needle (C) as shown in Figure 2a.

2A to 2D are diagrams for explaining a method of using the centrifugal container 11 shown in FIG. 1, and FIGS. 3A to 3C are various problems of the centrifugal container 11 used as described above. Figure is for explaining the problem of one of the drawings.

As shown in Figure 2a, in order to extract the PRP from the blood (B), first, the injection needle (C) of the syringe (A) with the blood collected through the water level control unit 19 side rubber member (17) Inject into the container (11). If all the blood (B) is injected into the container 11, the needle (C) is removed, and the container 11 is mounted on a centrifuge (not shown) to proceed with centrifugation. After the centrifugation, plasma P and red blood cells R are separated up and down as shown in FIG. 2B.

If the plasma (P) is separated from the red blood cells (R) in this way, by adjusting the position of the water level control unit 19, after raising the interface between the plasma (P) and red blood cells (R) to the opening hole 21, 2 The opening 23 is closed to block the opening 21 by the blocking plug 25a. (Fig. 2C)

Subsequently, when the container 11 is mounted in the centrifuge again as it is, the centrifuge separates the plasma P into platelet-poor plasma (PPP) and platelet deficient plasma (PPP) as shown in FIG. 2D. .

The PRP is heavier than the PPP and sinks to the lower portion of the first storage unit 13. Accordingly, in order to collect the PRP, the needle C of the syringe A is passed through the rubber member 17 of the sealing cap 15 to reach the lower portion of the first storage part 13 via the PPP layer. It should be pulled up by force.

By the way, the conventional blood centrifuge container 11 is configured as described above, inconvenient to use the needle (C) to inject blood or to extract the PRP, and in particular the fatal disadvantage by using the needle (C) Has That is, as illustrated in FIGS. 3A to 3C, the rubber debris 17a may be separated from the rubber member 17 during the process of puncturing the injection needle C through the rubber member 17. .

That is, as shown in FIG. 3B, the rubber member 17 moves in the direction of the arrow a with respect to the tip portion of the needle C while the needle C is passed through the rubber member 17. Some tissue of the member 17 may be torn and separated in the direction of the arrow b. As shown in FIG. 3C, the rubber member, which begins to tear as described above, is completely removed from the rubber member 17 at the moment when the tip portion of the injection needle C passes completely through the rubber member 17, thereby becoming rubber fragments 17a.

When the needle is inserted into the container 11, the rubber debris 17a may enter the inside of the container 11. In particular, when the size of the rubber debris (17a) is fine, it is difficult to find it is mixed in the blood or PRP, if the fine rubber debris (17a) is mixed in the finally obtained PRP, rubber debris when injecting PRP into the subcutaneous Since it is injected with PRP, serious side effects occur at the site.

Also, as shown in FIG. 2D, in order for the needle C to reach the PRP layer, the needle C must pass through the PPP layer. In this way, since the needle C must pass through the PPP layer, the PPP is transferred to the PRP layer in a state of being stuck on the needle, and the purity of the PRP is reduced by that amount.

Furthermore, even when the needle C is taken out to the outside after the suction of the PRP is completed, the tip portion of the needle must pass through the PPP layer, so that the PPP may penetrate into the needle during the extraction of the needle. Due to this problem, the needle C removed from the container 11 may be replaced with a new needle which is not used.

In addition, since the injection needle (C) must be used as described above, there is a high risk of infection. For example, in order to separate and analyze blood of an AIDS patient, an AIDS virus is transmitted to an operator if a needle is accidentally stuck in the process of adding blood into the container 11.

In addition, in the centrifugal container 11 having the above structure, since the first storage part 13 and the second storage part 23 are completely enclosed, and the atmospheric pressure does not reach, the infusion of blood and the inhalation of PRP are not smooth. According to the model, there is a container for centrifugation in which an air passage is formed, but the air passage serves as a passage for bacteria.

The present invention has been created to solve the above problems, it is possible to inject and inhale blood without using a needle, so that there is no risk of infection can be used with confidence, and can maintain the pressure in the sealed inner space at atmospheric pressure The purpose of the present invention is to provide a container for blood centrifugation that allows for smooth infusion and inhalation of blood.

In order to achieve the above object, the blood centrifugal container of the present invention is centrifugally rotated by a centrifugal separator in a state in which the blood to be centrifuged is accommodated therein, thereby separating and accommodating the internal blood into parts other than plasma and plasma. As; An upper case is closed by an airtight cap and a lower end is opened downwardly through a communication hole, and an upper case accommodating the separated plasma therein; A lower case coupled to the lower end of the upper case so as to be adjustable in position with respect to the upper case and whose lower end is opened; A lifting cap mounted on the lower end of the lower case to adjust a position of the lower case through position adjustment with respect to the lower case; A first check valve mounted on the elevating cap and opened by an external force and passing the collected blood into the lower case; An opening / closing part for opening or blocking a communication port of the upper case by following the position adjustment of the lower case with respect to the upper case; As to draw out the plasma contained in the upper case to the outside, one or more provided in the closure cap, the second check valve is opened by an external force, and installed in the inner space of the upper case, the upper end It characterized in that it comprises a suction tube is mounted to the second check valve and extends in the longitudinal direction downward.

In addition, the suction tube and the second check valve is formed in one set and a plurality are provided, any one of the suction tube of the plurality of suction tube extending relatively longer than the other suction tube, the lower end of the communication port It is characterized by reaching.

In addition, the closure cap, characterized in that the connection portion is coupled to the suction means for extracting the plasma from the upper case through the second check valve to the outside.

In addition, the second check valve; Located in the lower portion of the sealing cap and opened through the through-hole to the upper cap, the valve receiving portion for communicating with the upper end of the suction tube in the lower sealing cap, the valve receiving portion is installed to be liftable inside the, And an elevating member that is pushed by the draft means to open the through hole when the draft means is coupled to the connecting portion, and elastic means for supporting the elevating member upward to allow the elevating member to block the through hole. .

In addition, the lifting cap; A through passage is formed to open the inner space of the lower case downward, wherein the first check valve; An elevating member which is installed to the elevating passage, and is lifted by the blood injecting means to open the passage by fixing the blood injecting means used to inject the collected blood to the lower case side; It characterized in that it comprises an elastic means for elastically supporting the elevating member to cause the elevating member to block the passage when the external force is removed.

In addition, the closure cap; When the blood is injected or drawn out, it is characterized in that the pressure control unit for adjusting the pressure in the container to the atmospheric pressure is further provided.

In addition, the pressure control unit; Ring-type filter holder is installed through the sealing cap and opened up and down, and is mounted to the inside of the filter holder, characterized in that it comprises an antibacterial filter for passing air in the thickness direction.

In addition, the upper portion of the sealing cap, characterized in that the protective cap for blocking the contamination of the connection portion is detachably mounted.

The blood centrifugal container of the present invention as described above can be used safely and safely without injecting blood without using a needle, so it can be used safely without any concern about infection. It can maintain blood infusion and inhalation smoothly.

2A, 2B, 2C, 2D, and FIG. 3 are diagrams for explaining the problem of the centrifugal container shown in FIG.

Figure 4 is a perspective view of a blood centrifuge container according to an embodiment of the present invention.

FIG. 5 is a cutaway exploded perspective view illustrating the sealing cap and the first and second suction tubes supported therein as shown in FIG. 4 separately.

6 and 7 are diagrams for explaining the principle of plasma aspiration in the blood centrifuge container according to an embodiment of the present invention.

8 is a cross-sectional view showing the overall internal structure of the blood centrifuge container according to an embodiment of the present invention.

FIG. 9 is a perspective view illustrating the lifting member shown in FIG. 8 separately.

10 to 12 are diagrams for explaining the process of obtaining the PRP using the blood centrifuge container according to an embodiment of the present invention.

Hereinafter, one embodiment according to the present invention will be described in detail with reference to the accompanying drawings.

4 is a perspective view of a blood centrifuge container 31 according to an embodiment of the present invention.

As shown, the blood centrifuge container 31 according to the present embodiment, the upper case 33 and the upper case 33 having a cylindrical shape and the sealing cap 35 is fixed to the upper end, Screwed to the lower end of the lower case 47 that can adjust the relative position with respect to the upper case 33 through the axial rotation, screwed to the lower end of the lower case 47 and three-way valve 51 Lifting cap 49 leading to the lower case 47 by passing the blood through, and the protective cap 37 is fitted to the upper portion of the sealing cap 35 to protect the connection portion 35b and the pressure regulator 39 It includes. The upper case 33 and the lower case 47 is made of translucent acrylic or glass so as to check the state of the inside from the outside.

The upper case 33 and the lower case 47 communicate with each other through a communication port (33c in FIG. 8) formed at the lower end of the upper case 33.

First, the upper case 33 is an upper and lower open type tube having male threads (33f and 33e in FIG. 8) formed at upper and lower ends thereof, and screwed to the sealing cap 35 and the lower case 47. The sealing cap 35 is coupled to the male screw part 33f of the upper case to seal the inner space of the upper case 33.

In addition, the lower end of the upper case 33 is provided with a communication port (33c of FIG. 8) and the close contact portion (33g). This will be described with reference to FIG. 8.

The lower case 47 is a cylindrical member having a female screw portion 47e formed on an inner circumferential surface of an upper end portion thereof and a male screw portion 47c formed on a lower end portion thereof, and having the passage opening and closing portion 25 therein. The flow path opening and closing part 25 serves to open and close the communication port 33c.

The three-way valve 51, which is a component of the blood injection means used to inject blood into the lower case 47, collects the blood passing through the blood collection needle 53 and the tube 53a, It is for supplying into the centrifugal container 31 through the lifting cap 49.

The three-way valve 51 has a first port 51c to which the syringe cylinder 55 is coupled, a second port 51d connected to a first check valve (49r of FIG. 8) to be described later, and the tube. A body 51a having a third port (not shown) to which the 53a is coupled, and is installed at the side of the body 51a to connect the first port 51c and the third port, or a first port. The switching lever 51b connects the 51c and the second port 51d. Reference numeral 51e denotes a port fixing nut for fixing the second port 51d to the first check valve 49r.

Referring to the method of injecting blood into the container 31 using the three-way valve 51, first, the blood collection needle 53 is inserted into the blood vessel in the human body, and the first port 51 and the third Communicate the port. Thereafter, the syringe cylinder 55 is fixed to the first port 51, and then a piston (not shown) of the syringe is pulled out to provide a vacuum in the body 51a and the tube 53a to draw blood from the blood vessel. Serve

If the required amount of blood is collected inside the syringe cylinder 55 through the above process, the switching lever 51b is turned to block the third port, and the first port 51c and the second port 51d communicate with each other. Let's do it. In this state, when the blood in the syringe cylinder 55 is pushed into the first port 51c, the blood is injected into the container 31 through the second port 51d and the lifting cap 49.

The blood is supplied to the inside of the centrifugal container 31 without using an injection needle. (The blood collection needle 53 is not an injection needle that is plugged into the container 31.)

On the other hand, the sealing cap 35 is provided with a connecting portion 35b and the pressure adjusting portion 39. The connecting portion 35b is a cylindrical portion having a constant diameter, and the syringe cylinder 55 is inserted and fixed therein as shown in FIG. 6. The type of the connection part 35b may vary depending on the type of the syringe cylinder 55.

The lower part of each said connection part 35b is equipped with the 2nd check valve (45 of FIG. 6), respectively. The second check valve 45 is normally closed and is opened when the syringe cylinder 55 is coupled to the first connecting portion 35b.

In particular, the first suction tube 41 and the second suction tube 43 are fixed to the check valves 45, respectively. The first and

second suction tubes

41 and 43 extend downward as a pipe-shaped member having a constant diameter, so that the first suction tube 41 reaches the communication port 33c. The second suction tube 43 has a relatively shorter length than the first suction tube 41.

In addition, the pressure adjusting unit 39 is for adjusting the internal pressure of the upper case 33 and the lower case 35 to atmospheric pressure, and has an antibacterial filter (39b of FIG. 6) through which air can pass. The structure of the pressure regulator 39 will be described later with reference to FIG. 6.

In addition, the upper teburi portion of the sealing cap 35 is formed with a groove (35a). The groove 35a is a portion into which the lower end of the protective cap 37 is fitted. In addition, an internal thread 35n is formed on the inner circumferential surface of the sealing cap 35 to be coupled to the upper case 33.

FIG. 5 is a cutaway exploded perspective view separately illustrating the sealing cap 35 and the first and

second suction tubes

41 and 43 supported therefrom.

As shown in the drawing, two connecting portions 35b are formed side by side at the top of the sealing cap 35, and second check valves 45 are provided at the lower portions of the connecting portions 35b, respectively. The second check valves positioned below the respective connecting portions 35b have the same structure.

The second check valve 45 has a cylindrical shape and is upwardly opened to the connecting portion 35b through the through hole 35g, and has a through passage 35e and a coupling portion 35m formed at the lower end thereof. In the state supported by the part 35d, the elevating member 45a which can be lifted up and down in the valve accommodating part 35d, and the spring support 35f formed in the valve accommodating part 35d. And a spring 45b for upwardly supporting the elevating member 45a.

The elevating member 45a extends upwardly into the internal space of the connecting portion 35b through the through hole 35g, and a pressing portion 45e having a slit 45d formed at an upper end thereof, and the pressing portion 45e. It consists of a disk portion 45c integrally formed at the bottom of the) and having a predetermined thickness and diameter, and a spring support portion 45f which is positioned below the disk portion 45c and is inserted into the spring 45d.

The disk portion 45c is positioned below the through hole 35g and is elastically pressed upward by the action of the spring 45b to block the through hole 35g. The outer circumferential surface of the pressing portion 45e is spaced apart from the inner circumferential surface of the through hole 35g. Therefore, when the disk portion 45c descends and opens from the through hole 35g, the sealing cap 35 is opened up and down.

On the other hand, the first suction tube 41 is a holder (41a) is fixed to the coupling portion (35m) of the lower end of the valve receiving portion (35d), the upper end of the hollow coupled to the holder (41a) It consists of a tube body 41b. The tube body 41b communicates with the inside of the valve accommodation portion 35d through the holder 41a, extends in the longitudinal direction, and reaches a lower end thereof through the communication port 33c. The tube body 41b of FIG. 5 is bent in the middle so that the lower end of the tube body 41b can be as close as possible to the communication port 33c.

The second suction tube 43 is composed of a holder 43a coupled to the lower end of the connecting portion 35b, and a tube body 43b having an upper end fixed to the holder 43a and extending vertically downward. . The second suction tube 43 has the same configuration as the first suction tube 41, but the length of the tube body 43b is shorter than that of the tube body 41b of the first suction tube 41. The second suction tube 43 is also a suction means used to pull out the PPP or PRP contained in the upper case 33 to the outside.

Referring to the figure, the lifting member 45a accommodated in the valve accommodation portion 35d is elastically supported upward by the action of the spring 45b to block the through hole 35g. Able to know. In other words, the disk portion 45c is in close contact with the lower portion of the through hole 35g to block the through hole 35g.

In this state, when the spout portion 55a of the syringe cylinder 55 is press-fitted into the connecting portion 35b to move the pressed portion 45e downward, the disc portion 45c is opened from the through hole 35g. . That is, the inner space of the upper case 33 is opened to the top.

In particular, the spout portion 55a is brought into close contact with the inner circumferential surface of the connecting portion 35b as its outer circumferential surface tapers and enters the inside of the connecting portion 35b. In fact, the size of the spout portion 55a is designed so that the spout portion 55a can be in close contact with the inner circumferential surface of the connection portion 35b when the spout portion 55a is press-fitted into the connection portion 35b.

In any case, if the through hole 35g is opened by the spout portion 55a entering the connection portion 35b, the piston (not shown) inside the syringe cylinder 55 is pulled up and the syringe cylinder 55 is opened. Create a vacuum inside. When a negative pressure is applied to the syringe cylinder in this way, the negative pressure acts on the inside of the upper case 33, so that the PRP around the lower end of the tube body 41b rises up the tube body 41b and the second check valve 45. Then, it is transferred to the syringe cylinder 55 via the connecting portion 35b and the slit 45d. PRP is aspirated without inserting the needle into the upper case (33).

On the other hand, during the extraction process of the PRP, the pressure control unit 39 guides the outside air into the upper case 33 to maintain the pressure inside the upper case 33 to atmospheric pressure.

The pressure regulating portion 39 is in the form of a cylinder or a ring is fixed to the closure cap 35 and the filter holder (39a) to provide an internal passage, and installed inside the filter holder (39a) filter It is composed of an antibacterial filter (39b) for blocking the passage of the holder (39a), and a fixing ring (39c) for fixing the antibacterial filter (39b) to the filter holder (39a). The antimicrobial filter 39b maintains a flat unfolded state by the fixing ring 39c and passes air in the thickness direction thereof. Since the antimicrobial filter 39b has an antimicrobial treatment, bacteria do not pass through.

Referring to the drawings, the male screw portion 33e of the upper case 33 and the female screw portion 47e of the lower case 47 are screwed together, whereby the lower case 47 is coupled to the upper case 33. It can be seen. In particular, since the upper case 33 and the lower case 47 are screwed, for example, when the lower case 47 is axially rotated with respect to the upper case 33, the lower case 47 for the upper case 33 The relative position of can be adjusted. That is, the stopper 25a provided in the lower case 47 can be moved forward and backward with respect to the communication port 33c.

The blocking plug 25a is made of silicon and is supported by a plurality of holders 25c extending in the radial direction. A flow path 25b is formed around the blocking plug 25a.

In addition, a close contact portion 33g is formed at the lower end of the upper case 33. The close contact portion 33g has a seal ring between the outer circumferential surface and the inner circumferential surface of the lower case 47 as a portion in close contact with the inner circumferential surface.

On the other hand, the lifting cap 49, the coupling part 49a for screwing to the lower end of the lower case 47, the coupling part 49a is integrally housed in the lower case 47 A piston portion 49b having a through passage 49c in the central shaft portion is provided. The outer circumferential surface of the piston 49b is in close contact with the inner circumferential surface of the lower case 47 so that blood does not leak therebetween.

When the lifting cap 49 is axially rotated with respect to the lower case 47, the piston 49b slides while being in close contact with the inner circumferential surface of the lower case 47 and changes the internal volume of the lower case 47. Let's do it.

In addition, the first check valve 49r is provided in the through passage 49c so as to be openable and openable. The first check valve 49r is opened when the port fixing nut 51e of the blood injection means is filled in the adapter 49m, and is automatically shut off when the port fixing nut 51e is removed.

The first check valve 49r is embedded in the through passage 49c and fixed to the adapter 49m having a locking protrusion 49n formed on an outer circumferential surface of the lower end thereof, and capable of lifting up and down the upper portion of the adapter 49m. A lifting member 49f provided, and a spring 49d for elastically pressing the lifting member 49f toward the adapter 49m side. The spring 49d elastically supports the elevating member 49f in a state where the upper end thereof is caught by a spring support jaw 49e formed on the inner circumferential surface of the through passage 49c.

The adapter 49m is a hollow tube that provides a passage 49p for passing blood therein, and the inner diameter of the passage 49p narrows toward the spring 49d. Further, the locking projection 49n is a protrusion that is caught by a female thread formed on the inner circumferential surface of the port fixing nut 51e.

Therefore, when the port fixing nut 51e is engaged with the adapter 49m while the second port 51d is inserted into the passage 49p, the tip end of the second port 51e is inside the adapter 49m. Further, the lifting member 49f is pushed upwards, and is embedded in the upper end portion of the passage 49p and fixed. The first check valve 49r is opened.

FIG. 9 is a perspective view separately showing the elevating member 49f shown in FIG. 8.

As shown in the drawing, the elevating member 49f has a constant diameter and thickness and extends downwardly integrally with the disk portion 49g for sealing the upper end of the adapter 49m and integrally with the lower portion of the disk portion 49g. But it is made of a tapered rod portion (49k) becomes thinner toward the bottom.

The taper rod part 49k is made thinner toward the lower part to separate the taper rod part 49k from the inner peripheral surface of the adapter 49m. In this manner, the tapered rod portion 49k should be spaced apart from the inner circumferential surface of the adapter 49m so that the flow passage of blood is secured when the disk portion 49g is pushed upward.

In particular, the lower end portion of the tapered rod portion 49k is straight, longer than the diameter of the second port 51d, and is supported by the upper end portion of the second port 51d without entering the second port 51d.

10 to 12 are diagrams for explaining the process of obtaining a PRP using the blood centrifuge container 31 according to an embodiment of the present invention.

First, the injection plug 25a is spaced apart from the communication port 33c so that the

internal spaces

33b and 47b of the upper case 33 and the lower case 47 communicate with each other. The three-way valve 51 of the means is filled in the adapter 49m. As the three-way valve 51 is filled in the adapter 49m, the first check valve 49r is automatically opened.

If the three-way valve 51 is fixed to the adapter 49m, the syringe cylinder 55 is fixed to the first port 51c, the blood collection needle 53 is inserted into the blood vessel, and then the syringe cylinder 55 The internal piston (not shown) is pulled out to collect blood in the syringe cylinder 55. At this time, the first port 51c and the second port 51d should be blocked.

When a sufficient amount of blood is collected in the syringe cylinder 55, the switching lever (51b in FIG. 4) is rotated to connect the first and

second ports

51c and 51d to the inside of the syringe cylinder 55. Blood is injected into the centrifugal separation container 31 through the second port 51d and the first check valve 49r. The injected blood fills the inner space 47b of the lower case 47 and then goes up to the inner space 33b of the upper case 33 through the communication port 33c.

While the blood fills the centrifugal container 31, the air inside the container 31 escapes through the antibacterial filter 39b of the pressure regulating unit 39, so that resistance due to internal pressure is generated. I never do that.

If the blood B is filled in the centrifuge container 31 as shown in Fig. 11, the three-way valve 51 is removed from the adapter 49m, and then the container 31 is mounted in the centrifuge. Centrifuge is performed.

Through the centrifugation, if blood is separated into plasma P and red blood cells R (as shown in FIG. 2B), the elevating cap 49 is rotated so that the interface between the plasma and red blood cells is communicated with the communication port 33c. ), The relative position of the lower case 47 with respect to the upper case 33 is also adjusted to block the communication port 33c with the blocking plug 25a.

Subsequently, the centrifugal container 31 in the above state is once again subjected to a centrifuge to carry out the second centrifugation.

When the second centrifugation is performed, the plasma inside the upper case 33 is separated into PPP and PRP. Since PRP is heavier than PPP, it accumulates at the bottom of the upper case 33.

In the above state, while the spout of the syringe cylinder 55 is fixed to the connection portion 35b of the first suction tube 41, a vacuum force is formed in the syringe cylinder 55, and PRP is sucked out through the tube body 41b. do.

When the PRP is aspirated, external air enters the inside of the upper case 33 through the antibacterial filter 39b of the pressure regulating unit 39, and maintains the pressure of the upper case 33 at atmospheric pressure.

For reference, the PPP of the boundary layer portion between the PPP and the PRP is clearly smaller in platelets than the PRP, but still has a certain amount of platelets, so if the amount of separated PRP is expected to be insufficient (before drawing the PRP) Through the second suction tube 43, the lower end of the PPP, that is, the PPP of the boundary layer portion with the PRP may be sucked out for some time.

As described above, the blood centrifugal container 31 of the present invention includes the first and second check valves, the first and

second suction tubes

41 and 43, and the like. It can be sucked out.

As mentioned above, although this invention was demonstrated in detail through the specific Example, this invention is not limited to the said Example, A various deformation | transformation is possible for a person with ordinary knowledge within the scope of the technical idea of this invention.

Claims

Centrifugal rotation by means of a centrifugal separator in a state in which the blood to be centrifuged is accommodated therein, thereby separating and accommodating the blood therein into parts other than plasma and plasma;

An upper case is closed by an airtight cap and a lower end is opened downwardly through a communication hole, and an upper case accommodating the separated plasma therein;

A lower case coupled to the lower end of the upper case so as to be adjustable in position with respect to the upper case and whose lower end is opened;

A lifting cap mounted on the lower end of the lower case to adjust a position of the lower case through position adjustment with respect to the lower case;

A first check valve mounted on the elevating cap and opened by an external force and passing the collected blood into the lower case;

An opening / closing part for opening or blocking a communication port of the upper case by following the position adjustment of the lower case with respect to the upper case;

As to suck out the plasma contained in the upper case to the outside,

At least one sealing cap is provided, the second check valve is opened by an external force and the suction tube is installed in the inner space of the upper case, the upper end is mounted to the second check valve and extend in the longitudinal direction downward Blood vessel for centrifugation, characterized in that it comprises.
The method of claim 1,

The suction tube and the second check valve form a set and a plurality are provided,

The suction tube of any one of the plurality of suction tube is relatively longer than the other suction tube, the lower end reaches the communication port, characterized in that the container for blood centrifugation.
The method of claim 1,

The closed cap, the blood centrifuge container, characterized in that the connection portion is coupled to the suction means for drawing out the plasma from the upper case through the second check valve.
The method of claim 3,

The second check valve is;

Located in the lower portion of the sealing cap and opened through the through hole to the upper cap, the valve receiving portion for communicating with the upper end of the suction tube in the lower cap;

An elevating member installed in the valve accommodation portion so as to be lifted and lowered by the suction means when the suction means is coupled to the connecting portion to open the through hole;

And an elastic means for supporting the elevating member upwardly to cause the elevating member to block the through hole.
The method of claim 1,

The lifting cap is;

Through passages for opening the inner space of the lower case to the bottom is formed,

The first check valve is;

A lifting member which is installed to the lifting passage so as to be lifted and pushed by the blood injection means to open the passage by fixing the blood injection means used to inject the collected blood to the lower case side;

And elastic means for elastically supporting the elevating member to cause the elevating member to block the passage when the external force is removed.
The method according to any one of claims 1 to 5,

The closure cap;

When the blood is injected or drawn out, the blood centrifuge container further comprises a pressure adjusting unit for adjusting the pressure in the container to the atmospheric pressure.
The method of claim 6,

The pressure control unit;

A ring filter holder installed through the sealing cap and opened up and down;

And a antimicrobial filter mounted in the filter holder and passing air in the thickness direction thereof.
The method of claim 3,

The upper portion of the closed cap, the blood centrifuge container, characterized in that the protective cap for detaching the connection portion is detachably mounted.